Publications

the predominant cultivable flora of sound and carious human root surfaces.	little detailed knowledge exists about the composition of the human root flora. therefore, the predominant cultivable flora (pcf) was determined for samples of: (1) plaque from sound root surfaces (srs) in eight subjects without root caries, (2) plaque from incipient root lesions (irl) in eight subjects with root caries, and (3) carious material from advanced root lesions (arl) in nine other subjects with root caries. generally, one root surface was sampled per subject, and organisms-358, 512, a ...	1994	7983259
evaluation of the antimicrobial effectiveness of citric acid and sodium hypochlorite on the anaerobic flora of the infected root canal.	this study was designed to evaluate the antimicrobial effectiveness of 25% citric acid, compared with 2.5% sodium hypochlorite, after time intervals of 5, 15, 30 and 60 min. anaerobic bacterial species were isolated from infected root canals and categorized according to gram stain as gram-positive cocci, gram-negative cocci, gram-positive rods and gram-negative rods. the experimental method used was similar to that proposed by the german society for hygiene and microbiology (dghm) with slight mo ...	1994	7995646
the bacteriology of obstructive pneumonitis. a prospective study using ultrasound-guided transthoracic needle aspiration.	obstructive pneumonitis, the opacity that develops distal to an obstructing endobronchial lesion or external compression, is actually a combination of atelectasis, bronchiectasis with mucus plugging, and true parenchymal inflammation. in the majority of cases, it is usually not possible to determine whether infection is present or not from the radiographic findings alone. the aim of this study was to evaluate the bacteriology of obstructive pneumonitis and the influence of this result on the tre ...	1994	8004324
chemostat flow cell system: an in vitro model for the evaluation of antiplaque agents.	we developed an experimental in vitro model of dental plaque to assess the potential efficacy of antiplaque agents. the model used a chemostat, which provided a continuous source of 5 species of oral bacteria grown in an artificial "saliva-like" medium. this mixture was pumped through six flow cells, each containing two types of surfaces on which plaque formed and was subsequently measured. formation of bacterial plaque on hydroxyapatite surfaces was assessed by measurement of the dna and protei ...	1994	7983262
relationship of oral microflora with oral health status in parkinson's disease.	parkinson's disease (pd) patients report an increased craving for sweets, which may have an effect on microflora. we compared patients of pd who crave sweets with pd patients who do not. age- and sex-matched control subjects were used, with 14 subjects in each group. a plaque sample was taken from tooth #18 with a curette and placed into rtf, homogenized, and plated onto selective and non-selective media. microflora were expressed as % cfu's of total anaerobes. statistical analysis was performed ...	1994	7716701
single polymerase chain reaction for the detection of tetracycline-resistant determinants tet k and tet l.	oligonucleotide primers were used in polymerase chain reaction assays to detect tetracycline-resistant determinants tet k and tet l. forty-three isolates representing 11 genera carrying tet k and/or tet l determinants gave appropriate pcr products. the pcr products hybridized with labelled tet k or tet l probes, and differentiated the tet k from the tet l pcr products. we confirmed that two haemophilus aphrophilus carried the tet k determinant and two veillonella parvula carried the tet l determ ...	1994	7877638
[therapeutic possibilities in recurrent infantile tonsillitis].	the present study compares two therapy protocols in 40 children with recurrent tonsillitis. twenty of them were randomly treated with penicillin, and the remaining 20 received penicillin + metronidazole. clinical and microbiological assessment of the results was made. the association of penicillin and metronidazole was most efficient, probably due to its activity against streptococus pyogenes and anaerobic betalactamase-producing bacterias. the high failure rate in the penicillin group states th ...	1994	7873233
the effects of a 0.3% triclosan-containing dentifrice on the microbial composition of supragingival plaque.	144 subjects completed a 6-month, double-blind study which examined the effects of a 0.3% triclosan/2% copolymer/0.243% sodium fluoride dentifrice on the microflora of supragingival dental plaque. the subjects were randomly assigned to use, in an oral hygiene program, either the triclosan/copolymer/fluoride test dentifrice or a control dentifrice. the latter had the same formulation as the test dentifrice except it did not contain triclosan. supragingival plaque was collected from the buccal and ...	1994	8034778
anaerobic flora of the conjunctival sac in patients with aids and with anophthalmia compared with normal eyes.	relatively few investigations of anaerobic bacteria as ocular flora have been conducted, and their results have been contradictory. the conjunctival sacs of 22 normal subjects and of 14 patients with acquired immunodeficiency syndrome, and 22 anophthalmic sockets were cultured for anaerobic bacteria thirty-four (77.3%) of the 44 eyes of normal subjects harbored anaerobic bacteria; propionibacterium acnes was present in 28 eyes (63.6%), lactobacillus species in 6 eyes (13.6%), and veillonella spe ...	1994	8079632
increased in vitro activity of ceftriaxone by addition of tazobactam against clinical isolates of anaerobes.	a total of 461 clinical strains of anaerobes were tested using a broth microdilution test to determine the activity of the combination of ceftriaxone and tazobactam and other antimicrobials against these isolates. ceftriaxone was combined with tazobactam in ratios of 1:1, 2:1, 4:1, and 8:1 and twofold dilutions of ceftriaxone in constant concentrations to tazobactam of 2, 4, 8, 16, and 32 micrograms/ml. against beta-lactamase-producing strains of the bacteroides fragilis group, b. capillosus, an ...	1994	7851086
the oral gram-negative anaerobic microflora in young children: longitudinal changes from edentulous to dentate mouth.	eruption of primary teeth has a great influence on the oral environment by providing suitable niches for bacterial colonization. the composition of oral gram-negative anaerobic microflora was investigated in 21 young children (mean age 32 months) with primary dentition. the bacterial findings of samples were compared with those of the same children collected at their edentulous infant period (mean age 3 months). during the primary period, 2 samples were collected from each child: a sample with d ...	1994	7936718
in vitro activity of meropenem compared with imipenem, metronidazole, ampicillin, and ampicillin/sulbactam against anaerobes.	the aim of the present study was to compare the in vitro activity of meropenem (ici 194660, cas 96036-03-2) with imipenem, metronidazole, clindamycin, ampicillin and ampicillin/sulbactam against a variety of anaerobic bacteria using an agar dilution method. 423 clinical isolates were tested belonging to 70 species of 15 anaerobic genera. they included bacteroides fragilis (n = 62), bacteroides thetaiotaomicron (n = 45), prevotella bivia (n = 11), fusobacterium nucleatum (n = 12), clostridium per ...	1994	7945523
increased activity of a new chlorofluoroquinolone, bay y 3118, compared with activities of ciprofloxacin, sparfloxacin, and other antimicrobial agents against anaerobic bacteria.	a total of 435 clinical isolates of anaerobes were tested with a broth microdilution method to determine the activity of bay y 3118 compared with those of other agents against anaerobic bacteria. all strains of bacteroides capillosus, prevotella spp., porphyromonas spp., fusobacterium spp., clostridium spp., eubacterium spp., peptostreptococcus spp., and veillonella parvula were susceptible (mics of < or = 2 micrograms/ml) to bay y 3118. against the 315 strains of the bacteroides fragilis group, ...	1994	7979307
effect of proteolytic enzymes on the lysis and growth of oral bacteria.	previous studies have shown increased levels of proteolytic enzymes in affected periodontal sites. the aim of the present investigation was to evaluate the effect of proteolytic environments on the lysis and growth of selected oral bacteria associated with either healthy or diseased periodontal sites. the effect of trypsin, chymotrypsin and proteinase k on cell lysis was determined following incubation with bacteria, whereas the effect of the same proteolytic enzymes on bacterial growth was test ...	1994	7478762
fermentation of radiolabelled substrates by batch cultures of caecal microflora maintained in a continuous-flow culture.	glucose- and lactose-based media containing either 14c-labelled glucose, galactose, lactose or lactic acid were inoculated with anaerobic cultures of chicken caecal bacteria maintained for 121 d in a continuous-flow (cf) culture. the culture was previously shown to reduce salmonella colonization in the caeca of chicks inoculated with the culture and the reduction was associated with increases in volatile fatty acids. the distributions of 14c were determined among the fermentation products, espec ...	1995	7615424
characterization of inhibitory effects of suspected periodontopathogens on osteogenesis in vitro.	by using an in vitro bone-forming culture system, the chick periosteal osteogenesis (cpo) model, the direct effects on osteogenesis of sonicated extracts derived from oral bacteria were examined. both extracts from bacterial species having strong associations with periodontal diseases (porphyromonas gingivalis, actinobacillus actinomycetemcomitans, and prevotella intermedia, hereinafter referred to as suspected periodontopathogens) and extracts from species not correlated with periodontal diseas ...	1995	7642257
detection of tet(m) and tet(o) using the polymerase chain reaction in bacteria isolated from patients with periodontal disease.	the polymerase chain reaction was used to examine 114 tetracycline-resistant anaerobic and facultative anaerobic bacterial isolates from patients with periodontal disease for the tet(m) and tet(o) genes. a 740-base-pair fragment of the tet(m) gene was amplified from 84 of 114 isolates, and a 519-base-pair fragment of the tet(o) gene was amplified from 13 streptococcal isolates. six of 7 tetracycline-resistant isolates of veillonella spp. and tetracycline-resistant isolates of eubacterium spp. (n ...	1995	7675524
synergism of lactate and succinate as metabolites utilized by veillonella to inhibit the growth of salmonella typhimurium and salmonella enteritidis in vitro.	the inhibition of salmonellae growth by a veillonella bacterium isolated from the cecal contents of adult chickens was examined. the veillonella isolate was grown on an agar medium supplemented with 175 mumol of lactate or succinate/ml. either 0, 100, 125, 150, or 175 mumol of succinate/ml was added to the lactate medium; either 0, 100, 125, 150, or 175 mumol of lactate/ml was added to the succinate medium; and the ph of all media was adjusted to 6.0. agar overlays of veillonella cultures grown ...	1995	7677651
detection and incidence of the tetracycline resistance determinant tet(m) in the microflora associated with adult periodontitis.	subgingival plaque samples were collected from 68 patients with adult periodontitis, enumerated on trypticase-soy blood agar plates, with and without tetracycline at 4 micrograms/ml, and incubated anaerobically for 5 days. each different colony morphotype was enumerated, and a representative colony was subcultured for identification and examined for the tetracycline resistance gene tet(m). both pcr amplification and dna hybridization, using a fragment of tet(m) from tn1545, were used to detect t ...	1995	7730961
head and neck space infections in infants and children.	a retrospective study was performed in 117 children with head and neck space infections treated at the children's hospital of pittsburgh from january 1986 through june 1992. peritonsillar space infections were the most common (49%), followed by retropharyngeal (22%), submandibular (14%), buccal (11%), parapharyngeal (2%), and canine (2%) space infections. the most common pathogens isolated (n = 78) were the aerobes beta-hemolytic streptococcus (18%) and staphylococcus aureus (18%), the anaerobes ...	1995	7870436
[nosocomial infections in present-day traumatological-orthopedic hospitals].	nosocomial infection remains a significant problem in modern traumatology and orthopedics. staphylococcus prevails in its etiological pattern. the activation of microorganisms such as acinetobacter is noted. in addition to aerobic microorganisms, anaerobic bacteria, nonsporulating ones, play an important etiological role. anaerobic infection is seedy in 30.4% of patients' blood samples. great emphasis is placed on microbial adhesiveness in the pathogenesis of an infectious process. the authors' ...	1995	7626997
predominant microflora of severe, moderate and minimal periodontal lesions in young adults with rapidly progressive periodontitis.	the purpose of this investigation was to study the microflora of severe, moderate and minimal periodontal lesions, in young adults with rapidly progressive periodontitis (rpp). subgingival plaque samples were taken from 142 periodontal lesions in 10 young adults aging 25 to 35 years. the examination of the subgingival microflora indicated that certain species, including porphyromonas gingivalis, bacteroides forsythus, fusobacterium nucleatum, actinobacillus actinomycetemcomitans, and campylobact ...	1995	7722848
meningitis caused by mixed anaerobic species complicating tethered cord syndrome.		1995	8527587
comparison of periodontal disease in hiv seropositive subjects and controls (ii). microbiology, immunology and predictors of disease progression.	the aims of this study were to compare the prevalence of suspected periodontal pathogens in subgingival plaque from 29 hiv seropositive and 27 control subjects and to determine the association of these bacteria with periodontal destruction. subgingival plaque was collected from the mesiobuccal sites of all teeth, except 3rd molars. bacteria were identified and enumerated using non-isotopic whole chromosomal dna probes and a colony lift method. at baseline, hiv seropositive subjects had significa ...	1995	7560241
expression of the sodium ion pump methylmalonyl-coenzyme a-decarboxylase from veillonella parvula and of mutated enzyme specimens in escherichia coli.	the structural genes of the sodium ion pump methylmalonyl-coenzyme a (coa)-decarboxylase from veillonella parvula have recently been cloned on three overlapping plasmids (pjh1, pjh20, and pjh40) and sequenced. to synthesize the complete decarboxylase in escherichia coli, the genes were fused in the correct order (mmdadecb) on a single plasmid (pjh70). a dna region upstream of mmda apparently served as promoter in e. coli because expression of the mmd genes was not dependent on the correct orient ...	1995	7601825
a comparison of the microbial flora in carious dentine of clinically detectable and undetectable occlusal lesions.	it is not known whether the aetiology of occlusal hidden caries lesions (hcl) is identical to that of small visible lesions (svl). previous studies of the microflora of hcl suggest that relatively few species can be isolated. the aim of the present study was to compare the bacterial composition of dentine from 10 hcl and 17 svl in a population of children aged 8-18 years. the following bacteria were identified: actinomyces spp., mutans streptococci, streptococcus sanguis, streptococcus oralis, s ...	1995	7867050
a comparative study of glucose and galactose uptake in pure cultures of human oral bacteria, salivary sediment and dental plaque.	the ability to utilize glucose and the weaker sugar acidogen, galactose, was surveyed in salivary sediment, pooled dental plaque, and in pure cultures of the bacteria that numerically comprise most of the bacteria in these mixed microbial systems. except for a veillonella isolate, which showed no uptake of either sugar, glucose was utilized more rapidly than galactose by the 27 pure cultures tested and by both sediment and plaque. this sugar difference was also seen for two other measures of gly ...	1995	7487576
phylogenetic placement of dialister pneumosintes (formerly bacteroides pneumosintes) within the sporomusa subbranch of the clostridium subphylum of the gram-positive bacteria.	the nucleotide sequence of the 16s rrna gene of the type strain of dialister pneumosintes was determined. phylogenetic analysis revealed that this species belongs to the sporomusa branch of the clostridium subphylum of the gram-positive bacteria and should therefore be excluded from the family bacteroidaceae. within this branch, which encompasses several other gram-negative taxa, such as acidaminococcus, pectinatus, phascolarcobacterium, quinella, selenomonas, and zymophilus, dialister showed a ...	1995	7537075
bacteriology of skin and soft-tissue infections: comparison of infections in intravenous drug users and individuals with no history of intravenous drug use.	the bacteriology of cutaneous or subcutaneous abscesses (86 specimens) among intravenous drug users (ivdus) was compared with the bacteriology of abscesses (74 specimens) in patients with no history of intravenous drug use (non-ivdus). the ivdu abscesses yielded 173 aerobes and 131 anaerobes. staphylococcus aureus was the most common aerobe isolated (50% of specimens yielded this isolate), followed by "streptococcus milleri" (46%). the commonly encountered anaerobes were fusobacterium nucleatum ...	1995	7548575
studies into the microbial spectrum of apical periodontitis.	this study examined the variety of obligate and facultative anaerobic bacterial species recovered from cases of acute apical periodontitis. a total of 19 root canal samples and 24 periapical granuloma samples were taken from patients suffering pain and discomfort. bacteria were identified by applying the following techniques: culturing on various media, gram-staining and using commercially available biochemical test strips. in addition, prevotella intermedia and porphyromonas endodontalis were d ...	1995	8626206
treatment of extraoral sinus tracts from traumatized teeth with apical periodontitis.	when a draining lesion is encountered on the skin of the face, an endodontic origin should always be considered in differential diagnosis. non-surgical endodontic therapy, sometimes complimented by surgery, or extraction are the choices for the treatment of these cases. three cases of extraoral sinus tract on the chin caused by necrotic pulp of traumatized lower anterior teeth are presented. a paste consisting of calcium hydroxide and barium sulfate powder mixed with glycerin was used. usage of ...	1995	7641626
veillonella myositis in an immunocompromised patient.	infectious myositis is rather uncommon. when caused by anaerobic organisms, myositis is usually polymicrobial. trauma, ischemia, or a contiguous focus of infection is often an antecedent of myositis. we report a case of monomicrobial veillonella myositis in an immunocompromised patient. the infection responded to debridement and therapy with metronidazole.	1995	8527568
isolation and characterization of coaggregation-defective (cog-) mutants of streptococcus gordonii dl1 (challis).	streptococcus gordonii dl1 (challis) bears coaggregation-mediating surface adhesins which recognize galactoside-containing surface polysaccharides on streptococcus oralis 34, streptococcus oralis c104, and streptococcus sm pk509. fifty-nine spontaneously-occurring coaggregation-defective (cog-) mutants of s. gordonii dl1 unable to coaggregate with partner streptococci were isolated. six representative cog- mutants were characterized by their coaggregation properties with four actinomyces naeslun ...	1995	8519477
comparison of diagnostic tests for bacterial kidney disease in juvenile steelhead trout (oncorhynchus mykiss).	in order to accurately diagnose bacterial kidney disease caused by renibacterium salmoninarum in steelhead trout, kidney tissue from experimentally infected fish was evaluated using a commercially available enzyme-linked immunosorbent assay (elisa) test kit, fluorescent antibody (fa) testing, bacteriologic culture, and histopathology. seventy-five steelhead trout were randomly assigned to 1 of 4 groups and intraperitoneally inoculated with 0.15 ml saline (n = 20), 1 x 10(10) organisms/ml (n = 18 ...	1995	8580171
microbiological assessment of an improved stannous fluoride dentifrice.	recently, a new stabilized stannous fluoride (snf2) dentifrice (ssf) has been developed. the aim of the present work was to examine the antimicrobial activity of snf2, and to assess the long-term microbial safety of this dentifrice in a series of in vitro and clinical evaluations. results of in vitro time-kill experiments with representative oral bacteria demonstrated that snf2 exerts broad antimicrobial activity against both gram-positives and gram-negatives and, in particular, has potent activ ...	1995	8593200
microbial alterations in supragingival dental plaque in response to a triclosan-containing dentifrice.	a total of 325 subjects were entered into a double-blind, stratified 2-treatment clinical study that examined the effects of a dentifrice containing 0.3% triclosan, 2% gantrez copolymer and 0.243% sodium fluoride on supragingival dental plaque and gingivitis. a subset of 159 subjects including 72 men and 87 women participated in the microbiological component of this study, which was designed to detect shifts in supragingival bacterial species in response to triclosan. subjects were divided into ...	1995	8602338
antibacterial activity of the metabolic by-products of a veillonella species and bacteroides fragilis.	a veillonella species and bacteroides fragilis were isolated from the cecal contents of adult chickens. when growth on an agar medium supplemented with 0.4% glucose and adjusted to ph 6.5, mixed cultures containing veillonella and b. fragilis inhibited the growth of salmonella typhimurium; salmonella enteritidis, escherichia coli 0157:h7 and pseudomonas aeruginosa. decreasing the glucose concentration of the agar decreased the inhibitory activity of the mixed culture. mixed cultures grown on aga ...	1995	16887516
resistance of veillonella to hydrosoluble pristinamycins: a possible outer membrane barrier effect.	an in vitro study on 25 veillonella strains showed a consistent clindamycin susceptibility with resistance to erythromycin and to the hydrosoluble pristinamycins, quinupristin and dalfopristin and its 30:70 combination, the synergistin rp59500. double erythromycin-clindamycin disk tests did not show any inducible resistance pattern. the addition of 10 or 50 mug/ml of polymyxin b nonapeptide, an outer membrane permeabilizing agent, consistently reduced quinupristin and dalfopristin mics in most s ...	1995	16887512
[a preliminary study on the relationship between streptococcus and neisseria,veillonella in an artificial mouth model]	the viable cell count of monobacterial plaque of streptococcus and mixed bacterial plaque of streptococcus and neisseria veillonella,also the demineralization caused by the plaques were observed.the assumption that neisseria and veillonella might reduce demineralization of enamel caused by streptococcus was confirmed.the relationship between streptococcus and neisseria,veillonella were preliminarily found out.	1996	15160005
elimination of bacteraemia after dental extraction: comparison of erythromycin and clindamycin for prophylaxis of infective endocarditis.	erythromycin and clindamycin are currently recommended for antibiotic prophylaxis of infective endocarditis in predisposed patients allergic to penicillin undergoing oral invasive procedures. thirty-eight healthy patients were randomized to receive either erythromycin (1 g) or clindamycin (0.6 g) orally 1.5 h prior to dental extraction. blood samples for microbiological investigation were collected before, during and 10 min after surgery and were processed by lysis filtration under anaerobic con ...	1996	8722544
veillonella infections in children.	from 1974 to 1994, 2,033 specimens from children were submitted for cultures for anaerobic bacteria. eighty-three veillonella spp. were recovered from 83 children (4%). most veillonella species were recovered from abscesses, aspiration pneumonias, burns, bites, and sinuses. the infections were polymicrobial in 79 (95%) patients, but in 4 (5%) patients, veillonella species were recovered in pure culture. the predisposing conditions associated with the recovery of these organisms were previous sur ...	1996	8727920
effects of sublethal exposure to an antiseptic mouthrinse on representative plaque bacteria.	although the mechanism responsible for the clinical antiplaque efficacy of oral antiseptics is generally considered to be primarily one of bactericidal activity, it has been suggested that oral antiseptics may have additional effects on bacteria exposed to sublethal levels. studies reported herein, investigated the effects of sublethal levels of an essential oil-containing antiseptic mouthrinse (listerine antiseptic, warner-lambert co., morris plains, nj) on selected activities of representative ...	1996	8783049
clinical, microbiological and immunological profile of healthy, gingivitis and putative active periodontal subjects.	thirteen periodontally healthy subjects were monitored clinically for 6-12 months. clinical measurements at 6-weekly intervals included duplicate pd measurements, presence of plaque, redness, and bleeding on probing. baseline measurements consisted of 2 visits 1 wk apart. microbial samples were taken from 11 of the subjects who had completed at least 8 months of monitoring. levels of serum antibodies to 12 periodontal species were determined from 10 subjects. standard deviations of replicate pd ...	1996	8814590
the establishment of a community of oral bacteria that controls the growth of candida albicans in a chemostat.	the purpose of this study was to establish and identify a community of oral bacteria that controls the growth of candida albicans in the chemostat. the chemostat was operated under glucose-limiting conditions at a dilution rate of 0.05 h-1 and inoculated with a yeast-free suspension of a tongue scraping. after a steady state had been reached, it was inoculated with c. albicans to establish the yeast and determine whether its growth could be contained. the steady-state community comprised the spe ...	1996	8941776
effect of an antiseptic mouthrinse on salivary microbiota.	to evaluate the effect of rinsing with original formulation listerine antiseptic (la) on the level of viable salivary bacteria for periods up to 1 hour.	1996	9002796
shear bond strength, microleakage and antimicrobial properties of aelitebond.	to evaluate the shear bond strength (sbs) and microleakage (ml) to dentin as well as the antimicrobial action against five strains of oral bacteria of aelitebond single primer restorative system.	1996	9002802
veillonella parvula bacteremia without an underlying source.	veillonella parvula is an anaerobic gram-negative coccus that is part of the normal human flora. it has rarely been identified as a pathogen in humans, and the most frequently reported infection caused by v. parvula is osteomyelitis. we report a case of bacteremia unrelated to a central venous catheter and without an underlying source of infection.	1996	8940482
characterization of xanthine dehydrogenase from the anaerobic bacterium veillonella atypica and identification of a molybdopterin-cytosine-dinucleotide-containing molybdenum cofactor.	the molybdenum-containing iron-sulfur flavoprotein xanthine dehydrogenase from the anaerobic bacterium veillonella atypica has been purified approximately 800-fold with a yield of approximately 40% and a specific activity of approximately 70 micromol ferricyanide reduced x min(-1) x mg protein(-1) with xanthine as electron donor, which corresponds to approximately 30 micromol xanthine oxidized x min(-1) x mg protein(-1) with methylene blue as electron acceptor. the 129-kda enzyme was a non-coval ...	1996	8706691
[the effect of tinidazole in the treatment of adult periodontitis].	fifty-two adult periodontitis patients were treated by the tablets of tinidazole (tnz), and 23 patients treated by metronidazole (mnz) served as control group. the effective rate of tnz in adult periodontitis patients was 73.1%, which was significantly higher than that of the control group (43.5%). antimicrobial studies showed that the capability of tnz to kill the periodontal dominant anaerobic bacteria, especially b. gingivalis and b. melaninogenicus, was better than that of mnz. the minimal i ...	1996	9592262
the effect of chlorhexidine on defined, mixed culture oral biofilms grown in a novel model system.	in order to develop an improved method to evaluate antimicrobial agents for use in clinical dentistry, a constant-depth film fermenter (cdff) has been used to generate biofilms of fixed depth comprising nine species of bacteria commonly found in dental plaque in health and disease. these bacteria were grown together initially in a conventional chemostat which was used to inoculate the cdff over an 8 h period. medium was then supplied directly to the cdff and biofilms allowed to develop. the biof ...	1996	8760321
inhibition of salmonella typhimurium by the products of tartrate metabolism by a veillonella species.	the inhibition of the growth of salmonella typhimurium by a veillonella species grown on media supplemented with tartrate was examined. growth of salmonella typhimurium was not inhibited by the concentrations of products metabolized by veillonella cultures on media supplemented with 0 or 50 mmol l-1 of tartrate, but was inhibited on media supplemented with 100 or 150 mmol l-1 of tartrate. inhibition of salm. typhimurium was correlated with the increased production of acetate and propionate from ...	1996	8760327
purification and partial characterisation of the e14k mutant of flavodoxin from megasphaera elsdenii.		1996	8674684
adherence, accumulation, and cell division of a natural adherent bacterial population.	developing dental bacterial plaques formed in vivo on enamel surfaces were examined in specimens from 18 adult volunteers during the first day of plaque formation. an intraoral model placing enamel pieces onto teeth was used to study bacterial plaque populations developing naturally to various cell densities per square millimeter of surface area of the enamel (w. f. liljemark, c. g. bloomquist, c. l. bandt, b. l. philstrom, j. e. hinrichs, and l. f. wolff, oral microbiol. immunol. 8:5-15, 1993). ...	1996	8576054
three subtypes of the tet(m) gene identified in bacterial isolates from periodontal pockets.	the tet(m) genes were characterized from 84 isolates of 10 different bacterial species isolated from the periodontal pockets of 16 patients with periodontal disease. a 740 bp polymerase chain reaction product from the hypervariable region of the tet(m) structural gene was cleaved with the restriction enzymes alui and hinfi. three different restriction patterns were identified for each of the two enzymes. by dna sequencing, using a direct solid-phase automated sequencing method, the isolates coul ...	1996	9028254
crystallisation of the flavodoxin from megasphaera eldenii .		1996	8674647
incidence of anaerobes in ventilator-associated pneumonia with use of a protected specimen brush.	the role of anaerobic bacteria in ventilator-associated pneumonia (vap) has been little investigated. in this study we analyzed the incidence of anaerobes in patients with a first episode of bacteriologically documented vap (> 10(3)cfu/ml), using protected specimen brushes (psb). we particularly took care to preserve anaerobic conditions during transport and the microbiological procedure. two groups were considered: group a with anaerobic bacteria recovered from psb, with or without anaerobes, a ...	1996	8616556
anaerobic septic bursitis: case report and review.		1996	8722967
crystallisation of the flavodoxin from megasphaera eldenii.		1996	8674697
peripartum bacteremia with cdc group hb-5 (pasteurella bettyae).		1996	8783737
[anti-anaerobic activity of carbapenems].	the critical concentrations of sensitivity and resistance for meropenem versus anaerobic bacterias were analyzed. it is demonstrated the meropenem is a powerful inhibitor of the microorganisms of the bacteroides groups (mic90 < 1 mg/l), prevotella > porphyromonas, fusobacterium, veillonella, clostridium perfringens is inhibited at a mic < 0.06 mg/l. the mic of meropenem versus c. difficile is 2 mg/l. they are also highly susceptible to the propionibacterium, peptostreptococcus and peptococcus ty ...	1997	9410076
methylmalonyl-coa decarboxylase from propionigenium modestum--cloning and sequencing of the structural genes and purification of the enzyme complex.	methylmalonyl-coa decarboxylase catalyses the only energy-conserving step during succinate fermentation by propionigenium modestum: the decarboxylation of (s)-methylmalonyl-coa to propionyl-coa is coupled to the vectorial transport of na+ across the cytoplasmic membrane, thereby creating a sodium ion motive force that is used for atp synthesis. by taking advantage of the sequence similarity between the beta-subunits of other na+-transport decarboxylases, a portion of the p. modestum beta-subunit ...	1997	9428714
[a case of severe odontogenic infection with allergic skin reaction for beta-lactam antibiotics].	a 54-aged woman consulted with right buccal phlegmon caused by lower apical periodontitis. the primary chemotherapy using flomoxef induced allergic skin eruption. a decision was made for using clindamycin, levofloxacin and fosfomycin as chemotherapeutic agents. cultured organisms from the abscess revealed the mixed infection with streptococcus sanguis, veillonella sp., prevotella loescheii, wolinella spp. on 12th hospital day, her serum crp turned to negative. this case carried numerous implicat ...	1997	9209128
inhibition of listeria monocytogenes growth by veillonella cultured on tartrate medium.		1997	9310648
pcr-restriction fragment length polymorphism analysis of genes coding for 16s rrna in veillonella spp.	restriction fragment length polymorphism analysis of pcr-amplified 16s ribosomal dna (16s rdna pcr-rflp) was used to generate restriction profiles of the american type culture collection type strains of the genus veillonella, i.e., v. atypica, v. caviae, v. criceti, v. dispar, v. parvula, v. ratti, and v. rodentium. whole-cell protein profiles were obtained by sodium dodecyl sulfate-polyacrylamide gel electrophoresis for comparative purposes. the 16s rrna genes were amplified by pcr, and rflp an ...	1997	9336941
post-caesarean septicaemia in kandang kerbau hospital, singapore, 1993-1995.	we reviewed all documented cases of septicaemia following caesarean deliveries in kandang kerbau hospital between 1st january 1993 to 31st december 1995. there were 22 cases of septicaemia among 8201 caesarean births, and hence the incidence is 2.7:1000. there were 45,412 deliveries, and the overall caesarean section rate was 18.1%. among the 22 documented cases of septicaemia which came under this study, the most common clinical conditions found were endomyometritis (7 cases), urinary tract inf ...	1997	10968108
early echo-guided percutaneous aspiration of peripheral lung abscesses in children: report of two cases.	a 9 year-old boy and a 7 year-old girl suffered from fever and chest pains before they were brought to chang gung children's hospital. chest radiographs and computed tomography scans showed lung abscesses with pleural attachment, and using echo-guidance, percutaneous aspirations were done which confirmed the growth of fusobacterium sp, and veillonella parvula respectively, both of which are susceptible to penicillin g. the fever and chest pains subsided within 24 hours after the procedure. we su ...	1997	9151468
[a study of anaerobic infection in maxillofacial region].	to analyse the anaerobic infection of maxillofacial surgery and estimate the efficacy of antianaerobic therapy, 45 patients were divided into two groups, tinidazole group and metronidazole group. bacterial culture was positive before treating in all cases. there were bacillus melaniogenicus, veillonella, peptococcus and peptostreptococus, etc. there was excellent efficacy in the treatment of maxillofacial anaerobic infection by tinidazole intravenously. after treatment, the result of bacterial e ...	1997	9868121
variations in the predominant cultivable microflora of dental plaque at defined subsites on approximal tooth surfaces in children.	the distribution and composition of the resident microflora were determined in approximal gingival margin plaque from 21 premolars extracted from schoolchildren (mean age 12.0 +/- 1.8 yr). indigo carmine (5% w v) was used to visualize plaque to facilitate sampling. about 1 mm2 of plaque was removed from sites away from (a), to the side of (s), and below (b) the contact area. plaque samples were dispersed, serially diluted, and cultured on selective and non-selective agar media. an average of sev ...	1997	9134122
prosthetic valve endocarditis due to veillonella dispar: successful medical treatment following penicillin desensitization.		1997	9142818
penicillin resistance in veillonella.		1997	9145906
identification of a putative histidine base and of a non-protein nitrogen ligand in the active site of fe-hydrogenases by one-dimensional and two-dimensional electron spin-echo envelope-modulation spectroscopy.	the active h-cluster of the fe-hydrogenases from megasphaera elsdenii and desulfovibrio vulgaris (strain hildenborough) has been investigated with one- and two-dimensional pulsed epr spectroscopy. in both complexes the coordination of a nitrogen-containing ligand was found. the unusual quadrupole interaction parameters (d. vulgaris: quadrupole coupling constant, k = 1.20 mhz, asymmetry parameter eta = 0.32, m. elsdenii: k = 1.23 mhz, eta = 0.25) indicate a non-protein type of nitrogen and are co ...	1997	9346288
evaluation of the bbl crystal anaerobe identification system.	the bbl crystal anaerobe (anr) identification system was evaluated, and the results were compared with those from conventional anaerobic methods. we tested 322 clinically significant anaerobic bacteria according to the manufacturer's instructions. the system identified correctly 286 of 322 (88.8%) of the anaerobic bacteria tested. of these, 263 of 322 (81.7%) were identified correctly on initial testing and 49 were identified correctly only to the genus level; on repeat testing, 23 of 49 (46.9%) ...	1997	9399517
detection and quantification of poultry probiotic bacteria in mixed culture using monoclonal antibodies in an enzyme-linked immunosorbent assay.	murine monoclonal antibodies were used in an enzyme-linked immunosorbent assay (elisa) for the detection and quantification of selected probiotic bacteria present in a continuous-flow competitive exclusion culture known to be effective at reducing chicken cecal and crop colonization by salmonella typhimurium. veillonella, enterococcus avium and s. typhimurium were grown anaerobically in batch culture of viande levure broth in pure culture and mixed culture. the mixed cultures produced significan ...	1997	9506283
a comparison of susceptibility results of the bacteroides fragilis group and other anaerobes by traditional mic results and statistical methods.	antimicrobial susceptibility is compared by mic values reflecting activity by weight and/or percentage of strains resistant. this study establishes these parameters in vitro in the traditional fashion and extends analysis statistically for various groups of anaerobic bacteria. mode mic, mic50, mic90 values and percentage of strains resistant indicated comparable activity for most of the beta-lactams. geometric and arithmetic mics suggested ceftizoxime to be the most active beta-lactam. statistic ...	1997	9096180
differentiation of veillonella atypica, veillonella dispar and veillonella parvula using restricted fragment-length polymorphism analysis of 16s rdna amplified by polymerase chain reaction.	veillonella atypica, veillonella dispar and veillonella parvula cannot be reliably distinguished by conventional phenotypic tests, including the api zym test. in this study, restricted fragment-length polymorphism (rflp) analysis of 16s rdna amplified by polymerase chain reaction (pcr) was used to generate restriction profiles of the type strains of v. atypica, v. dispar and v. parvula and 20 veillonella strains isolated from oral sites. 16s rrna gene sequences from isolated genomic dna samples ...	1997	9573809
a microbiological study of pre- and postoperative apicoectomy sites.	there is little information on the microbiology of periapical lesions, and no data on the residual microbial flora in the periapex, if any, after apicoectomy procedures. hence, 64 patients treated by apicoectomy procedures were prospectively studied to assess the bacterial flora in the periapex and to evaluate the residual bacteria in postoperative apicoectomy sites. of the 64 lesions studied, 14 (22%) were sterile and 50 (78%) yielded bacteria preoperatively. bacteria could be recovered from 28 ...	1997	9552822
comparison of batch culture growth and fermentation of a poultry veillonella isolate and selected veillonella species grown in a defined medium.	the objective of this study was to develop a defined medium for quantitating nutritional requirements and fermentation products of a poultry cecal isolate of veillonella and to compare these parameters with representative veillonella species. the poultry isolate is one of 29 organisms from a continuous-flow culture that has been shown to be effective against salmonella colonization in broilers. when the veillonella species were grown in anaerobic batch culture, propionate and acetate were the on ...	1997	16887614
production and characterization of monoclonal antibodies to a veillonella species from a continuous-flow culture system of chicken cecal bacteria.	administering native intestinal flora to newly hatched chicks protects against cecal salmonella colonization, and is known as competitive exclusion. continuous-flow culture systems have been used to maintain defined competitive exclusion cultures. we have recently demonstrated that such a stable continuous-flow culture, cf3, contains 29 bacterial strains representing ten genera. broiler chicks treated with cf3 are protected against salmonella colonization of the ceca. such protection is correlat ...	1997	16887559
peptidases of the rumen bacterium, prevotella ruminicola.	prevotella (formerly bacteroides) ruminicola is a numerous rumen bacterium which plays a significant role in the metabolism of proteins and peptides in the rumen. measurement of the hydrolysis of synthetic aminopeptidase substrates by sonicated extracts and whole cells of different species of rumen bacteria indicated that p. ruminicola had the greatest range and specific activity of dipeptidyl peptidases among the species tested. streptococcus bovis hydrolysed some dipeptidyl peptidase substrate ...	1997	16887560
in vitro activity of levofloxacin against a selected group of anaerobic bacteria isolated from skin and soft tissue infections.	the in vitro activity of levofloxacin was compared to the activities of ofloxacin, ciprofloxacin, ampicillin-sulbactam (2:1), cefoxitin, and metronidazole for a selected group of anaerobes (n = 175) isolated from skin and soft tissue infections by using the national committee for clinical laboratory standards-approved wadsworth method. ampicillin-sulbactam and cefoxitin inhibited 99% of the strains of this select group, levofloxacin and ofloxacin inhibited 73 and 50%, respectively, at 2 microg/m ...	1998	9559829
comparative anti-anaerobic activity of men 10700, a penem antibiotic.	the in vitro activity of men 10700, a new penem, has been compared with that of metronidazole, clindamycin, ciprofloxacin, co-amoxiclav, imipenem and three third generation cephalosporins against 120 strains of anaerobes. the organisms tested comprised clostridium perfringens, clostridium difficile, bacteroides fragilis and speciated members of the genera fusobacterium, veillonella and peptostreptococcus. men 10700 showed activity similar to that of imipenem, and was more potent than metronidazo ...	1998	9916909
the microflora associated with human oral carcinomas.	both local and systemic infections may complicate the morbidity of patients with oral malignant neoplasms, particularly those presenting intraorally. this study investigated the microbial contents of the biofilms present on the surfaces of oral squamous cell carcinomas. biofilm samples were obtained from the central surface of the lesions in 21 patients (20 male, 1 female) aged 52.8 (+/- 8.2) years, and from contiguous healthy mucosa, before any antibiotic therapy or any tumour treatment. all le ...	1998	9813727
subgingival microbiota of shallow periodontal pockets in individuals after head and neck irradiation.	this study aimed at investigating the subgingival plaque microorganisms of shallow pockets (< or = 5 mm) in subjects who previously received irradiation in the head and neck region for treatment of nasopharyngeal carcinoma. direct microscopy and anaerobic culture were used. subgingival paper point samples were taken from 6 tooth-sites (one/sextant) per subject for direct microscopy (n = 108). another set of paper points was taken from the deepest of the previously selected sites (one per subject ...	1998	9573815
antimicrobial activity of magnolol and honokiol against periodontopathic microorganisms.	magnolol (1) and honokiol (2), main compounds from the stem bark of magnolia obovata thunb., were evaluated for an antimicrobial activity against periodontopathic microorganisms, porphyromonas gingivalis, prevotella gingivalis, actinobacillus actinomycetemcomitans, capnocytophaga gingivalis, and veillonella disper, and a cytotoxicity against human gingival fibroblasts and epithelial cells. our results indicate that magnolol and honokiol, although less potent than chlorhexidine, show a significan ...	1998	9619121
two fatal cases of veillonella bacteremia.		1998	9512189
the effect of herbal extracts in an experimental mouthrinse on established plaque and gingivitis.	the purpose of the present study was to establish in vitro the inhibiting effect of a herbal extract mixture on a selected number of micro-organisms and to test in vivo the effect of a mouthwash containing 6.3 mg/ml herbal extract mixture on plaque and gingivitis as compared to a minus active control mouthrinse. the herbal extract was a mixture of: juniperus communis (juniper), urtica dioca (nettle), achillaea millefolium (yarrow); 1:1:1. in the study, in-vitro, the effect of pure herbal extract ...	1998	9650877
semiquantitative bacteriology of closed odontogenic abscesses.	pus samples from twenty-three dentoalveolar abscesses were collected by needle aspiration and examined by direct inoculation technique using 6 different aerobic and anaerobic agar plates. for aerobic culture, sheep blood agar and chocolate agar (kyokuto pharmaceutical co., tokyo, japan) were used. for anaerobic culture, four different media, 1) brucella hk agar with hemolyzed rabbit blood and defibrillated sheep blood, 2) paramomycin-vancomycin brucella hk agar with hemolyzed rabbit blood, 3) ph ...	1998	9667143
study of root caries in an artificial mouth.	the object of this project was to develop a laboratory model system to study root and enamel caries. model plaque biofilms containing consortia of four (streptococcus mutans, streptococcus sobrinus, actinomyces naeslundii, and lactobacillus rhamnosus) or six (s mutans, s sobrinus, s mitis, a naeslundii, l rhamnosus, and veillonella dispar) species of caries pathogens were developed in a artificial mouth. caries lesions similar to those found in vivo were produced on enamel and dentine blocks and ...	1998	9676475
analysis of ph-driven disruption of oral microbial communities in vitro.	previously, a mixed culture chemostat system was used to demonstrate that the ph generated from carbohydrate metabolism, rather than carbohydrate availability per se, was responsible for the shifts observed in the oral microflora which are associated with high carbohydrate diets and the development of dental caries. the aim of this study was to determine more accurately the microbially generated ph at which such shifts occurred. nine oral bacteria were grown in three independent chemostats, and ...	1998	9745120
[in vitro activity of 10 antibiotics including pristinamycin and its two components (rp 12536 and 27404) against strict anaerobes].	the activities of the pristinamycin and its two components (rp 12536 and rp 27404) were investigated using the reference agar dilution method m11a3, on 175 anaerobic strains in comparison with that of erythromycin, clindamycin, metronidazole, amoxycillin either alone or combined with clavulanic acid, piperacillin, cefoxitin, cefotetan and cefotaxime. beta-lactamase production was detected for all the 55 b. fragilis group strains and 8/12 prevotella and 2/18 fusobacterium strains, respectively. o ...	1998	9769928
development of an anaerobic bacterial leakage model.	the majority of bacteria associated with infections of endodontic origin are strict anaerobes. the purpose of this study was to develop an endodontic microleakage model using strict anaerobic bacteria in a two-chamber system. nine species of anaerobic bacteria were tested for viability and detection by either turbidity or color change of the broth. a survey of ph chromogenic substrates revealed that bromcresol purple (ph 5.2 = yellow, ph 6.8 = purple) could be used as a chromogenic indicator to ...	1998	9641124
influence of the maternal vaginal microbiota on the oral microbiota of the newborn.	the present study was conducted on 50 expectant mothers and the newborns, with the aim of observing if any, the relationship, acquisition and survival of the maternal vaginal microbiota to the oral microflora after birth. the oral cavity was sterile in 6% of the newborns. the differences between the counts of s. aureus, klebsiella, lactobacillus, ps. aeruginosa and veillonella sp. in the mother and in the child were of statistical significance. no statistically significant difference was observe ...	1998	9796502
microbial complexes in subgingival plaque.	it has been recognized for some time that bacterial species exist in complexes in subgingival plaque. the purpose of the present investigation was to attempt to define such communities using data from large numbers of plaque samples and different clustering and ordination techniques. subgingival plaque samples were taken from the mesial aspect of each tooth in 185 subjects (mean age 51 +/- 16 years) with (n = 160) or without (n = 25) periodontitis. the presence and levels of 40 subgingival taxa ...	1998	9495612
infective endocarditis caused by veillonella of dental origin.	the purpose of this investigation was to examine whether veillonella could cause transitory bacteriemia and endocarditis in both pure and mixed cultures when the port of entrance for infection was made in rats' incisors. incisors of 54 male zgr: whistar conventional rats were inoculated with pure culture of veillonella (18 animals) and with mixed culture of s. mutans and veillonella (18 animals). remaining 18 incisors (the control group) were treated with saline solution. the animals were sacrif ...	1998	9951138
[a bacteriology analysis from infected root canals of human deciduous teeth]	objective: to provide a basis for therapy and prevention, the flora of infected root canals of human deciduous teeth was analyzed.methods: by immediate-enzyme method, the bacteria compositions of 22 cases with infected root canals of deciduous teeth, including 8 cases with acute periapical inflammation and 14 with chronic ones, were examined. results: among 240 strains bactera, 200 strains were obligate anaerobes, belonging to the genera peptostreptococcus, bacteriodes, veillonella, eubacterrum, ...	1998	15071650
effect of the addition of fumarate on methane production by ruminal microorganisms in vitro.	the effect of fumarate used as a feed additive on the reduction of methanogenesis in the rumen was evaluated by in vitro experiments. the addition of fumarate to the culture of mixed ruminal microorganisms that were fermenting hay powder and concentrate reduced methane production. most fumarate was metabolized to propionate, and a slight increase was noted in other volatile fatty acids. fumarate was utilized by mixed bacteria but not by mixed protozoa. fibrobacter succinogenes, selenomonas rumin ...	1999	10212465
the effects of mouth rinses and dentifrice-containing magnesium monoperoxyphthalate (mmpp) on oral microflora, plaque reduction, and mucosa.	the effects of a magnesium monoperoxyphthalate (mmpp) mouth-rinse, with or without sodium lauryl sulphate (sls), and an mmpp dentifrice, on salivary counts of bacterial flora and yeasts, and on supragingival plaque scores were investigated in 131 healthy oral candida carriers over a 9 week double blind study. there were no changes in the salivary counts of bacteria studied (anaerobes, streptococci, fusobacteria, actinomyces, viellonella) in the test or placebo groups. a significant increase in s ...	1999	10223394
the influence of specific foods and oral hygiene on the microflora of fissures and smooth surfaces of molar teeth: a 5-day study.	a group of 20 students, harbouring >10(4) mutans streptococci per millilitre of saliva, was enrolled into the study. models for sampling, reproducibly, the dental plaque present in specific sites (fissure and smooth surface) on the dentition were developed and validated. withdrawal of normal oral hygiene procedures for only 1 day resulted in approximately 10-fold increases in the number of micro-organisms recovered from both sites. the effect of supplementing the subjects' diets with particular ...	1999	10460958
phylogenetic characterization of centipeda periodontii, selenomonas sputigena and selenomonas species by 16s rrna gene sequence analysis.	the nearly complete 16s rrna gene sequences for oral gram-negative anaerobic motile bacteria, centipeda periodontii, selenomonas sputigena and selenomonas species (formerly s. sputigena type strain), were determined in order to unveil their relationship to other oral motile bacteria. to determine the phylogenetic characterization of these bacteria, their 16s rrna gene sequences were obtained and compared with those from the ribosomal sequence databases previously reported. the 16s rrna gene sequ ...	1999	10464949
donor-to-recipient transmission of bacteria as an unusual cause of mediastinitis in a heart transplant recipient.	we present a 54-year-old male heart transplant recipient who developed mediastinitis caused by klebsiella oxytoca and veillonella species. culture of the donor's bronchus also grew k. oxytoca and a veillonella species. pulsed-field gel electrophoresis revealed that the k. oxytoca isolates had identical banding patterns. this case illustrates transmission of pathogenic bacteria via a contaminated organ.	1999	10064219