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distribution of xanthine oxidase and xanthine dehydrogenase specificity types among bacteria.a diverse collection of xanthine-metabolizing bacteria was examined for xanthine-, 1-methylxanthine-, and 3-methylxanthine-oxidizing activity. both particulate and soluble fractions of extracts from aerobically grown gram-negative bacteria exhibited oxidation of all three substrates; however, when facultative gram-negative bacteria were grown anaerobically, low particulate and 3-methylxanthine activities were detected. gram-positive and obligately anaerobic bacteria showed no particulate activit ...1977863854
affinity chromatography of antiserum to a gram negative organism.an immunoadsorbent method is described for the purification of antibody directed against gram negative bacteria. two anion exchange materials were compared for their ability to immobilize whole cells of veillonella alcalescens, a gram negative oral bacterium. purified antibody preparations were applied to the columns and subsequently eluted with various combinations of desorbing buffers. the quantity of recovered antibody was measured and its activity assayed, using a microagglutination techniqu ...1977864265
nitrate-reductase electron-transport cofactors in veillonella alcalescens.studies on the effects of inhibitors of the nitrate-reducing activity of veillonella alcalescens extracts suggest the participation of a naphthoquinone, a b-type cytochrome, and non-heme iron in electron transport to nitrate. a nitrate-reductase-deficient mutant displayed a longer doubling time and a decreased molar growth yield on nitrate media. this mutant was phenotypically restored by the addition of molybdate to the growth medium, giving evidence for the functioning of molybdenum in the nit ...1977922606
erythromycin and anaerobic infection.the theoretical basis of the use of erythromycin in peri-operative prophylaxis after bowel surgery will be discussed and the results of the use of erythromycin in such patients and the sensitivity to the antibiotic of isolates from human peritonitis described. this work will be related to results obtained in a rabbit experimental model for the study of peritonitis.1977601557
bacteremia after upper gastrointestinal endoscopy.during 24 months, 200 upper gastrointestinal endoscopies were performed on 193 patients. blood cultures were obtained before and five and 30 minutes after the procedure using thiol (50 ml) and trypticase soy broth (100 ml) media. the mean endoscopic time was 34 minutes. sixteen patients developed bacteremia (8%). twelve groups of microorganisms were detected in positive blood cultures: streptococcus (5 species), lactobacillus sp, veillonella alcalescens, staphylococcus aureus, staph epidermidis, ...1977404974
adherence of veillonella species mediated by extracellular glucosyltransferase from streptococcus salivarius.the effect of extracellular products from streptococcus salivarius on sucrose-dependent adherence to smooth surfaces by other oral bacteria was studied in vitro. strains of streptococcus mitis, streptococcus pyogenes, and veillonella parvula without innate ability to adhere to a steel wire were able to do so when incubated with sucrose and cell-free culture fluid from s. salivarius strains 9759, 25975, cnii, and mepi. these culture fluids synthesized more adherent material and water-insoluble gl ...1977591064
rabbit polymorphonuclear leukocyte migration in vitro in response to lipopolysaccharides from bacteroides, fusobacterium and veillonella.puriified lipopolysaccharides (lps) from strains of bacteroides, fusobacterium and veillonella incubated with guinea pig serum, were tested for chemotatic activity against rabbit polymorphonuclear leukocytes (pmns) in modified boyden chambers. comparisons were made to a salmonella lps (s. enteriditis s-795). submicrogram amounts of lps induced positive chemotaxis, and a typical dose-response relationship up to certain dose levels was observed. the difference in chemotactic activity between the v ...1977605803
rabbit polymorphonuclear leukocyte migration in vivo in response to lipopolysaccharides from bacteroides, fusobacterium and veillonella.subcutaneously implanted chambers in rabbits were used for testing the migration of polymorphonuclear leukocytes in response to injected lps isolated from strains of bacteroides, fusobacterium and veillonella. a salmonella lps was used as reference endotoxin. no differnece in chemotactic activity between the veillonella lps and lps from salmoneila was found. fusobacterium lps whoed insignificantly lower chemotactic capacity than the salmonella lps. the bacteroides lps were all significantly less ...1977605804
lethality for mice and chick embryos, pyrogenicity in rabbits and ability to gelate lysate from amoebocytes of limulus polyphemus by lipopolysaccharides from bacteroides, fusobacterium and veillonella.phenol-water extracted lipopolysaccharides (lps) from veillonella, fusobacterium nucleatum, bacteroides fragilis and bacteroides melaninogenicus were lethal for mice and 11-days-old chick embryos, pyrogenic in rabbits, and gelated limulus amoebocyte lysate. mouse lethality was considerably enhanced by actinomycin-d. in all test systems the endotoxin activity of veillonella and fusocbacterium lps was comparable to that of lps from salmonella enteritidis, which was included as a reference endotoxi ...1977564596
a semiselective medium for the isolation of veillonella species from the mouth. 1977845267
anaerobic ocular infections. 1977878140
pelvic abscess associated with repeated recovery of veillonella. 1977900203
dietary effects on the composition of fecal flora of rats.a long-term animal feeding experiment was conducted to compare the effect of meat and wayne laboratory chow diets on the composition of rat fecal flora. fecal bacteria were enumerated on selective media under both aerobic and anaerobic conditions. intrarectal administration of n-methyl-n' -nitro-n-nitro-soguanidine (mnng) affected the count only on the phenylethylalcohol and veillonella-neomycin agars, whereas a slightly higher number of anaerobes appeared in the feces of rats that were treated ...197716345222
reliable sampling method for analysis of the ecology of the human alimentary tract.an intubation method has been developed that allows removal of a sample of human intestinal fluid within a short period of time, that avoids contamination, and that minimizes exposure of the sample to air. preliminary results obtained with this method have shown that the stomach and duodenum are essentially sterile and that the bacterial population present in the remainder of the small intestine is similar to that described by previous workers except that veillonella species were encountered fre ...1978623454
faecal flora of south indian infants and young children in health and with acute gastroenteritis.the faecal flora of 29 healthy infants and young children was compared with that of 49 children of similar age and socio-ecomonic status with acute gastroenteritis. in the healthy children the most common organisms in the faeces were bifidobacteria, veillonellae, enterobacteria and enterodocci with anaerobes outnumbering aerobes. most members of the noraml faecal flora were present in the diarrhoeal stools, but anaerobes were signigicantly reduced in number and enterobacteria were significantly ...1978660639
effects of prostaglandin on the antigen- and mitogen-driven responses of peripheral blood lymphocytes from patients with adult and juvenile periodontitis. 1978699390
chemotypes of veillonella lipopolysaccharides.lipopolysaccharides (lps) were isolated by phenol-water extraction from 34 strains of veillonella, and examined by paper chromatography and colorimetric methods for the presence of neutral sugars, amino sugars and 2-keto-3-deoxy-octonate (kdo). several preparations were also examined for neutral sugars by gas liquid chromatography. the lps had in common glucosamine, galactosamine, l-glycero-d-manno-heptose glucose and kdo. most lps contained galactose, and a few rhamnose. d-glycero-d-manno-hepto ...1978665243
bacteriology of human experimental gingivitis: effect of plaque age.twenty-five subjects with previously excellent hygiene and healthy gingiva developed heavy plaque accumulations and bleeding or nonbleeding gingivitis about certain papilla after 21 days of no oral hygiene. gingival marginal plaque about a single papilla was collected at 0, 1, 2, and 3 weeks of no oral hygiene in each subject. the plaque was dispersed, serially diluted, and plated on mm10 sucrose agar in an oxygen-free atmosphere. from 50 to 100 colonies from a single high-dilution plate were ch ...1978711336
ticarcillin disodium in anaerobic infections.twenty-five patients were treated with ticarcillin disodium, 18 of whom had anaerobic infections that included pleuropulmonary infections (seven), mandibular osteomyelitis (four), perirectal abscess (two), sepsis, primary site unknown (one), liver abscess (one), pelvic abscess (one), decubitus ulcer (one), and synergistic gangrene (one). seven had no anaerobic infections. three had anaerobic septicemia. culture results included anaerobes: peptococci (ten), peptostreptococci (ten), bacteroides fr ...1978718311
[deletion pattern of enzymes in fermentation system and the relevent metabolism of carbon source in veillonella, a strict anaerobe indigenous to the mouth (author's transl)]. 1978295328
[nitrate reduction in human saliva. (4) reciprocal action of oral microorganisms on nitrate reduction by veillonella in vitro (author's transl)]. 1978389324
microflora of the cervix during normal labor and the puerperium.the normal flora of the cervix was studied in 50 healthy women in labor and on the third day postpartum. aerobic bacteria were the exclusvie isolates in 25% of these women, and 75% had anaerobic bacteria in addition to aerobes. anaerobes were always found in association with aerobes. the most frequent isolate of bacteroides was bacteroides melaninogenicus, found in 20% of the women during labor and 14% during the puerperium. bacteroides fragilis was distinctly absent during labor but was found i ...1978351071
symbiotic relationship of veillonella alcalescens and streptococcus mutans in dental plaque in gnotobiotic rats. 1978272950
microbiota of gingivitis in man.a study on the predominant cultivable microorganisms inhabiting gingival crevices affected with a chronic gingivitis was carried out using the roll tube culture technique. samples were obtained from nine individuals 25--42 years of age. gram-positive rods make up 29.1% of the isolates and included mainly actinomyces naeslundii, actinomyces israelii, and actinomyces viscosus. streptococcus mitis and streptococcus sanguis together made up 26.8% of the cultivable organisms. peptostreptococcus avera ...1978276916
[gas chromatography analysis of volatile monocarboxylic aliphatic acids (c2 to c6) of 15 strains of veillonella of oral origin].a qualitative and quantitative study has been carried out on 15 strains of the veillonella genus, isolated in the oral cavity. following two methods of preparation of samples for injection in a chromatograph gl, we have identified among the volatil monocarboxylic aliphatic acids from c2 to c6 the glucose fermentation products of these different strains. with the same methods, we studied in parallel these products from strains a.t.c.c. 10790-17444 (veillonella parvula) and 17445-17446 and 17447 ( ...1978279541
a microbiological study of old plaque in occlusal fissures of human teeth. 1978282009
a reliable test for differentiation and presumptive identification of certain clinically significant anaerobes.a modification of the slide catalase test was evaluated to determine its reliability for the presumptive identification of certain anaerobic gram-negative and gram-positive organisms isolated from clinical specimens. a total of 650 fresh isolates was evaluated. growth from a trypticase blood agar plate or schaedler's agar with laked blood was transferred to a slide and a drop of 15% h2o2 with tween 80 added. veillonella alcalescens, proprionibacterium species, peptococcus species, and bacteroide ...1978339709
[transfer and metabolism of nitrate, with special reference to recent problems on human body and the oral flora (author's transl)]. 1978396320
[in vitro activity of ornidazole against anaerobic organisms. differences seen in certain strains in relation to the partial pressure of oxygen. (author's transl)]. 1978209402
purification and characterization of acetate kinase from veillonella alcalescens atcc 17748. 197830411
magnetic coupling of the molybdenum and iron-sulphur centres in xanthine oxidase and xanthine dehydrogenases.magnetic interaction between molybdenum and one of the iron-sulphur centres in milk xanthine oxidase lowe, lynden-bell & bray (1972) biochem. j. 130, 239-249 was studied further, with particular reference to the newly discovered mo(v) e.p.r.(electron-paramagnetic-resonance) signal, resting ii lowe, barber, pawlik & bray (1976) biochem. j. 155, 81-85. e.p.r. measurements at 35ghz near to 4.2k showed that the interaction has the same sign at all molybdenum orientations and is ferromagnetic. the pr ...197825647
8-hydroxyquinolines with the potential for long-term anticaries activity: design, synthesis, and in vitro evaluation.8-hydroxyquinolyl benzoate and 8-hydroxyquinolyl para-(fluorosulfonyl)benzoate have been synthesized and evaluated in vitro as antimicrobial and antiplaque agents. both compounds inhibited the growth of cultures of the following genera: streptococcus, staphylococcus, actinomyces, lactobacillus, veillonella, and candida. minimum inhibitory concentrations ranged from 0.98 to 62 microgram/ml. extracted human teeth pretreated with 1% solutions of either compound and rinsed with distilled water exhib ...197898543
the relationship between caries index and stimulation of lymphocytes by streptococcus mutans in mothers and their neonates. 1978283738
oxygen metabolism by the anaerobic bacterium veillonella alcalescens. 197831846
properties of membrane adenosine triphosphatase of the obligately anaerobic bacterium veillonella alcalescens.some properties of membrane atpase activity in veillonella alcalescens were examined. mg2+ is required for the activity of the enzyme, and ca2+ also activates the enzyme to some degree. of the nucleotide triphosphates, gtp and itp were hydrolyzed to a lesser extent than atp. the apparent km for atp hydrolysis was 0.25 to 0.63 mm. adp inhibited the enzyme and the kinetic data of its inhibition showed that the presence of adp resulted in positive cooperativity. the enzyme activity was strongly inh ...1978149119
the presence of antibody-coated anaerobic bacteria in asymptomatic bacteriuria during pregnancy.quantitative anaerobic culture of urine samples obtained from 593 pregnant women by suprapubic bladder aspiration was performed to establish the involvement of anaerobic bacteria in asymptomatic urinary tract infections. the fluorescent antibody (fa) test was applied to the sediments of bladder aspirates to determine the site of infection. anaerobic bacteriuria (greater than or equal to 10(4) microorganisms/ml of urine) was found in 34 patients, of whom five were fa-positive. these anaerobes wer ...1979118996
endotoxins of anaerobic gram-negative rods.endotoxic lipopolysaccharides (lps) isolated from fusobacterium are similar to those of salmonella with respect to chemical composition, o-antigenic specificity and endotoxic activity. bacteroides lps are highly atypical with respect to the chemical composition, and their endotoxic activity is low. o-antigenic specificity is present. b. fragilis lps is chemotactic for polymorphonuclear leukocytes in vitro and in vivo. the chemotactic activity is brought about by activation of the alternative com ...197988759
the role of bacteroides melaninogenicus and other anaerobes in periodontal infections.recent taxonomic and anatomical studies of dental plaque associated with periodontal health and disease have demonstrated that differences in the microbial populations in plaque may be responsible for the initiation and progression of disease. the consistent isolation of large numbers of anaerobic and capnophilic bacteria from the depths of periodontal lesions has suggested an important role for these organisms. bacteria that have been isolated include capnocytophaga (bacteroides ochraceus), oth ...197944923
bacteriological study on the saliva of healthy adults. 1979106144
human complement activation by lipopolysaccharides from bacteroides oralis, fusobacterium nucleatum, and veillonella parvula.the properties of different lipopolysaccharide (lps) preparations to induce c3 conversion in human serum was studied by means of crossed immunoelectrophoresis. c3 conversion by the alternative pathway was evaluated after calcium depletion, and lipid a-dependent activation was measured by means of inhibition with polymyxin b sulfate. lps from bacteroides oralis converted co mainly via the alternative pathway, whereas lps from fusobacterium nucleatum and veillonella parvula const pronounced lipid ...1979121108
[study on the mechanism of nitrite production in the saliva]. 1979297704
the microbial composition of human incisor tooth plaque. 1979299146
survival of crevicular microorganisms in an artificial culture medium. 197943361
acetate kinase from veillonella alcalescens. purification and physical properties.acetate kinase (atp:phosphotransferase e.c.2.7.2.1) has been purified to a high state of purity from veillonella alcalescens. the native enzyme had a molecular weight of 88,000, as determined by sephadex g-150 gel filtration. the molecular weight of the monomeric enzyme, estimated from sodium dodecyl sulfate-polyacrylamide gel electrophoresis, was 42,000. the enzyme was determined to be a homodimer from the amino acid composition and the results of trypsin digestion and cyanogen bromide cleavage ...1979216674
acetate kinase from veillonella alcalescens. regulation by succinate and substrates.the kinetic properties of acetate kinase from veillonella alcalescens were investigated. in the presence of high concentrations of nucleotide both forward and reverse reactions were observed. in the presence of succinate the degree of cooperativity between subunits of the homodimer decreased, i.e. the hill coefficient, n, decreased from 2.5 to 1.4 for acetyl phosphate in the presence of succinate. at low substrate concentrations hyperbolic kinetic data were observed with succinate. we have propo ...1979221506
comparison of three methods of susceptibility testing of bacteroidaceae, peptococcaceae and other anaerobes to doxycycline.the in vitro activity of doxycycline against 147 strains of gram-positive and gram-negative anaerobes was determined by broth dilution, agar dilution and agar diffusion tests. the strains were isolated from clinical specimens in 1977. saccharolytic bacteroides strains (39 b. fragilis, 6 b. thetaiotaomicron, 4 b. vulgatus) showed broth dilution mics of less than or equal to 0.0625-4.0 microgram/ml after 5 h incubation and mics of less than or equal to 16 microgram/ml after 15 h incubation at 37 d ...1979543353
bacteria beneath composite restorations--a culturing and histobacteriological study.the occurrence, viability and identification of the microbial flora under composite fillings using an anaerobic technique were studied. class v cavities were prepared on clinically healthy buccal surfaces of 7 contralateral pairs of premolars in children 11--15 years of age. after preparation, rubber dam was applied and one cavity in each pair of teeth was washed with water blasted dry with air and filled with adaptic. the other cavity was washed with a cavity cleaner (tubulicid) and a cavity li ...1979393051
experimental gingivitis in odu plaque-susceptible rats. iii. toxic activity of the rat dental plaque.the toxicity tests of the dental plaque from odu plaque-susceptible rats showed strong lethal effect on mice, and abscess forming effect on guinea-pigs. bacterial cells isolated from the rat dental plaque also showed strong toxicity on both animals and capillary permeable activity on rabbits. among these bacterial cells, corynebacterium showed the strongest toxic effects on these animals. these facts suggested an important role of the dental plaque on initiation and development of gingivitis and ...1979289757
acute appendicitis and bacteroides fragilis.bacteria belonging to the bacteroides fragilis group (b. fragilis, b. ovatus, b. vulgatus, b. distasonis, b. thetaiotaomicron and b. uniformis) were cultured in quantities of less than or equal to 10(5) c.f.u./ml from the excised appendix from 30 patients. twenty-two patients (group i) had an acute purulent appendicitis (three with perforation), four were classified as slightly inflamed cases (group 2) and four had a normal appendix (group 3). the b. fragilis group of bacteria dominated among th ...1979379991
the effect of nalidixic acid on microaerophilic and anaerobic bacteria. special study of clostridia.study of the sensitivity of 141 strains of anaerobic and microaerophilic bacteria to nalidixic acid shows that few bacteria are inhibited by low concentrations (veillonella, eikenella, most of the clostridia). nalidixic acid appears to be bactericidal with respect to clostridium perfringens, and its point of attack in the dna is probably different from that of metronidazole.1979232341
subgingival microflora of periodontal patients on tetracycline therapy.samples of subgingival plaque were collected from periodontal patients receiving two different tetracycline treatment regimens following conventional periodontal therapy. four patients had received an oral dose of 1000 mg/day for 2 weeks and nine had received 1000 mg/day for 1 week followed by 250 mg/day for extended time periods. the latter regimen is similar so that commonly prescribed for the chronic skin disease, acne vulgaris. taxonomic characterization of plaque isolates indicated that the ...1979294454
maternal t-lymphocyte response and gingivitis in pregnancy.the exacerbation of a chronic gingivitis during pregnancy was confirmed in a group of 24 pregnant patients who were observed at the 14th and 30th weeks of pregnancy and postpartum. this exacerbation occurred despite a fall in the amount of plaque accumulation and confirmed that during pregnancy some factor in addition to plaque is involved in the increased inflammation. using in vitro methods, it was shown that during pregnancy there is a depression of maternal t-cell responsiveness and it is su ...1979312316
periodontal therapy in humans. i. microbiological and clinical effects of a single course of periodontal scaling and root planing, and of adjunctive tetracycline therapy.the present results showed that maarked and long-lasting changes in the subgingival microflora associated with periodontal disease could be achieved by a single course of periodontal treatment. immediately following therapy, the total number of subgingival organisms decreased 10- to 100-fold and the proportions of cultivable gram negative organisms and anaerobic organisms generally decreased 3- to 4-fold or more. after treatment, most periodontal pockets were populated by a scant microflora pre ...1979385821
the prevalence of anaerobic bacteria in suprapubic bladder aspirates obtained from pregnant women.the incidence of asymptomatic bacteriuria due to aerobic, facultative anaerobic and strictly anaerobic bacteria during pregnancy was determined in 185 patients admitted for complications of pregnancy, using suprapubic bladder aspiration. anaerobic bacterial counts of 103 per ml urine or higher were detected in 6.4 per cent of the specimens. the incidence of anaerobic bacteriuria seems comparable to that of bacteriuria due to aerobic and facultative anaerobic micro organisms. in our group of pati ...1979388942
a modified tube method for the cultivation and enumeration of anaerobic bacteria.a new type of tube (the lee tube) has been developed for use in the cultivation and enumeration of obligate anaerobes. the lee tube is a double-walled, screw-capped tube which allows the formation of a thin cylinder of agar medium between the two walls. anaerobiosis is achieved through deoxygenation of the deep cylinder of agar during sterilization, a minimum of head space, and use of a reducing agent to absorb oxygen introduced during the inoculation procedure. for several species of clostridiu ...1979396020
effect of intestinal bacteria on incidence of liver tumors in gnotobiotic c3h/he male mice.the effect of intestinal microflora on liver tumorigenesis was studied in gnotobiotic c3h/he male mice monoassociated, diassociated, or polyassociated with the following strains of intestinal bacteria: escherichia coli, streptococcus faecalis, bifidobacterium adolescentis, bifidobacterium infantis, clostridium indolis, c. paraputrificum, c. perfringens, c. innocuum, c. nexile, c. ramosum, c. clostridiiforme, bacteroides multiacidus, bacteroides fragilis, veillonella alcalescens, v. parvula, and ...1979292809
[difference in nitrate reductase activity between veillonella alcalescens and veillonella parcula (author's transl)]. 1979297483
in vitro activity of sodium fusidate against anaerobic bacteria.the microtiter broth dilution method was employed to determine the in vitro susceptibility of 525 recent clinical isolates of anaerobic bacteria to sodium fusidate. the minimal inhibitory concentrations of sodium fusidate ranged from </=0.06 to 1.0 mug/ml for 155 strains of anaerobic gram-positive rods and 130 strains of anaerobic gram-positive cocci. minimal inhibitory concentrations ranging from </=0.06 to 32 mug/ml were observed for 240 strains of anaerobic gram-negative rods. among the latte ...1979485124
recovery of anaerobic bacteria from pediatric patients. a one-year experience.during 1975, 115 speciments from the soft tissues, body fluids, and purulent sites in 105 pediatric ward and nursery patients were submitted to the clinical laboratory for anaerobic study, and 75% of the cultures were positive. in most instance specimens were collected in anaerobic transport tubes containing peptone yeast extract and glucose. in the laboratory, samples were processed by the anaerobic holding jar method. nineteen different anaerobic organisms were isolated; the average number of ...1979495592
chemotaxis or migration inhibition of rabbit peritoneal polymorphonuclear leukocytes caused by chemoattractants at various concentrations.purified lipopolysaccharide (lps) from veillonella incubated in normal rabbit serum was tested for chemotactic activity on rabbit polymorphonuclear leukocytes (pmns) in modified boyden chambers. in doses above those giving optimal response (over-optimal dose), a decrease of the pmn migration activity was found. this decrease also correlated well with an increase in the migration inhibition of the pmns as demonstrated with the capillary tube assay. the pmn chemotactic factor isolated from lps-ind ...1979525354
the effects of azathioprine and levamisole on lymphocyte stimulation.azathioprine at 10 micrograms per ml caused approximately a 50% inhibition of phytohaemagglutinin (pha), purified protein derivative (ppd) or veillonella induced in vitro lymphocyte stimulation. this suppression was significantly reduced in lymphocyte cultures from patients undergoing an eight week course of levamisole therapy. azathioprine-induced suppression was also reversed when culturing lymphocytes in the presence of levamisole. on the other hand, levamisole in vitro failed to reverse the ...1979527269
synthesis of alpha-ketoglutarate by reductive carboxylation of succinate in veillonella, selenomonas, and bacteriodes species.evidence for reductive carboxylation of succinate to synthesize alpha-ketoglutarate was sought in anaerobic heterotrophs from the rumen and from other anaerobic habitats. cultures were grown in media containing unlabeled energy substrates plus [14c]succinate, and synthesis of cellular glutamate with a much higher specific activity than that of cellular asparate was taken as evidence for alpha-ketoglutarate synthase activity. our results indicate alpha-ketoglutarate synthase functions in selenomo ...1979533772
anaerobic isolates in chronic suppurative otitis media. 1979538681
inhibitory activity of streptococcus mitis against oral bacteria.the antagonistic properties of three strains of streptococcus mitis were investigated. they were found to inhibit a wide range of oral bacteria; gram-positive and gram-negative, facultative and anaerobic species being susceptible. the s. mitis strains were shown to be producing hydrogen peroxide, this being partially responsible for the aerobic inhibitory activity. a second inhibitory factor(s) was also produced, aerobically and anaerobically, although this could not be isolated. a limited chara ...1979551241
factors affecting adhesion of bacterial to a tooth in vitro.the initial adhesion of oral bacteria to a tooth in vitro was examined. the organisms were grown in broth with and without sucrose, and suspensions made either in broth or a modified ringer's solution. the tooth used was either dry or coated with natural or synthetic saliva. adhesion was determined by counting organisms removed from the tooth surface by simple washing or by sonication. it was found that the firmest bonding occurred when a dry tooth was immersed in a suspension of bacteria in rin ...1979551240
nitrite reduction in veillonella alcalescens.nitrite reduction was examined in veillonella alcalescens c-1, and obligate anaerobe with an atp-yielding nitrate-reducing system. hydrogen donors for nitrite reduction included hydrosulfite, hydrogen gas, and pyruvate, but not pyridine nucleotides, in the presnece or absence of flavins. pyruvate-linked nitrite reduction was not inhibited by 4,4,4-trifluoro-1-(2-thienyl) 1,3-butanedione, dicoumarol, or 2-heptyl-4-hydroxy-quinoline-n-oxide. the noninvolvement of membrane-bound factors was support ...1979422515
skin and soft tissue polymicrobial infections from intravenous abuse of drugs.skin and soft tissue infections were studied in 21 seriously ill narcotic addicts who had been admitted to hospital. subcutaneous abscesses were present in 14 patients; cellulitis was noted in 3, pyomyositis in 2 and necrotizing fasciitis in 2. in four patients there was septicemia. infections in 14 patients (66.6 percent) were associated with anaerobic bacteria, which were the exclusive isolates in 6 patients. in seven patients (33.3 percent) isolates were exclusively aerobic bacteria and in ei ...1979425502
fatal acute bacterial myocarditis after dentoalveolar abscess.a 19 year old woman presented with chest pain after a dental extraction for a dentoalveolar abscess. electrocardiographic and serum isoenzyme changes were consistent with acute anterior myocardial infarction. at autopsy bacteria were demonstrated, within the myocardium in the absence of a myocardial abscess or endocarditis. this case illustrates the occurrence of isolated acute bacterial myocarditis after a dental extraction.1979443182
[short-term oral chemoprophylaxis before intestine surgery. quantitative determination of bacteria and fungi in stool specimens (author's transl)].the methods of quantitative analysis of aerobe and anaerobe microbes and fungi stool specimens are described. the results of the studies in health people are compared to the results in patients undergoing surgical treatment of intestinal tract. a group of these patients received neomycin and bacitracin orally as short-time chemoprophylaxis to diminish possible woundinfection and/or sepsis. after oral medication germs as bifidobacterium, bacteroides and clostridium (not cl. perfringens) are reduc ...1979452770
surgical treatment of infected right atrial myxoma. 1979453988
[infections by anaerobic sporeless bacteria]. 1979551585
occurence of veillonella and neisseria in the niches of the human oral cavity.samples collected from the dental surface, gingival sulcus, dorsum of the tongue and from the cheek of ten adults (18 to 32 years) were investigated to identify the veillonella and neisseria species present by applying the taxonomic criteria suggested in the latest edition of bergey's manual. the various niches contain both v. parvula and v. alcalescens, but with the predominance of strains which do not fit precisely the described catalase and putrescine requirements. eighty-nine out of 100 isol ...1979461857
[first steps in the bacterial colonization of the digestive tract of neonates (author's transl)].meconium samples obtained from 14 children aged 18 to 51 h were subjected to a quantitative differential analysis. the meconium of one of the children (47 h old) was sterile; in those of the 11 infants aged less than 47 h the dominant flora was composed of either enterobacteria, mainly escherichia coli sensitive to usual antibiotics, or of streptococci. the genus acinetobacter constituted the dominant flora in one case only. staphylococcus, corynebacterium, clostridium (c. perfringens), bacteroi ...1979475219
changes in lactate-producing and lactate-utilizing bacteria in relation to ph in the rumen of sheep during stepwise adaptation to a high-concentrate diet.changes in the numbers and types of lactate-producing and lactate-utilizing bacteria in the rumen of sheep were followed during stepwise adaptation from a low- to a high-concentrate diet. the mean numbers of bacteria increased after each change in diet when increasing amounts of maize grain were substituted for maize stover. a surge in number of amylolytic bacteria always preceded an increase in lactate-utilizing bacteria, and with the final diet containing 71% grain and molasses the two groups ...197916345431
inhibition of nitrate reduction in some rumen bacteria by tungstate.tungstate prevented the formation of active nitrate reductase in growing rumen bacteria capable of nitrate reduction, but did not directly inhibit the enzyme activity of all strains tested.19807190809
[reciprocal in vitro actions of streptococcus mutans, actinomyces and veillonella: a simplified model for carbohydrate metabolism in plaque].different acids can be found in vitro, if glucose is metabolized in plaque or by different combinations or oral bacteria: 1. streptococcus mutans and actinomyces do not show interactions concerning metabolism and formed acids. 2. streptococcus mutans and veillonella after short incubation time produce lactate; after long time of incubation acetic and propionic acid are main products and lactic acid diminishes. 3. actinomyces and veillonella react similar to streptococcus mutans and veillonella. ...19806935027
surface vs core-tonsillar aerobic and anaerobic flora in recurrent tonsillitis.specimens from both the surface and the core of tonsils from 23 children with recurrent tonsillitis were cultured for aerobic and anaerobic microorganisms. mixed aerobic and anaerobic flora were obtained from all patients. the predominant anaerobic isolates were bacteroides sp, fusobacterium nucleatum, anaerobic gram-positive cocci, and eubacterium sp. the predominant aerobic isolates were alpha-hemolytic streptococci, staphylococcus aureus, beta-hemolytic streptococci, and haemophilus sp. beta- ...19807411827
complications of sinusitis in children.eight patients (6 to 15 years of age) who had periorbital cellulitis and other complications of sinusitis were studied. both ethmoid and maxillary sinusitis were present in four patients, frontal sinusitis in two, and ethmoid sinusitis and pansinusitis in one patient each. subdural empyema occurred in four patients, in one case accompanied by cerebritis and brain abscess and in another by meningitis. periorbital abscess was present in two children who had ethmoiditis. alveolar abscess in the upp ...19807432842
adherence of streptococcus salivarius hb and hb-7 to oral surfaces and saliva-coated hydroxyapatite.we compared the binding of streptococcus salivarius hb and the mutant hb-7 to oral surfaces in vivo. mutant hb-7 does not aggregate with saliva nor does it bind to buccal epithelium, but it does retain its ability to coaggregate with veillonella and fusobacterium. at 1 h after inoculation into the oral cavity of six volunteers, significantly more s. salivarius hb than hb-7 cells were found adhering to the buccal mucosa (p < 0.05) and to a cleaned tooth surface (p < 0.01); there was no significan ...19807439971
molybdate and sulfide inhibit h2 and increase formate production from glucose by ruminococcus albus.h2 production from glucose by ruminococcus albus was almost completely inhibited by 10(-5) m molybdate only when sulfide was present in the growth medium. inhibition was accompanied by a significant increase in the production of formate. extracts of molybdate-sulfide-grown cells did not contain hydrogenase activity. active enzyme in extracts of uninhibited cells was not inhibited by the molybdate-sulfide-containing growth medium. the results indicate that a complex formed from molybdate and sulf ...19807369826
do anaerobes cause urinary tract infection? 19806101823
determination of the specificity of plasma cells for bacterial antigens in situ.we developed a method for determining the specificity of plasma cells for bacterial antigens in situ. the method consists of applying rhodamine-labeled whole bacteria to frozen sections of tissue in which inflammation had been induced by injection of the same bacterial strain. after appropriate washings, the tissue sections were briefly treated with a fluorescein-labeled antiserum to endogenous immunoglobulins to detect plasma cells. plasma cells which could recognize bacterial somatic antigens ...19806155341
comparison of moxalactam (ly127935) and cefotaxime against anaerobic bacteria.the in vitro activities of moxalactam (ly127935 [6059s]) and cefotaxime were compared with those of cefoxitin, cefamandole, cefuroxime, carbenicillin, and penicillin by agar dilution susceptibility testing of a variety of anaerobic bacteria. moxalactam proved to be the most active agent tested against bacteroides fragilis and other species of the b. fragilis group. moxalactam and cefotaxime showed activity similar to the other drugs against the remaining species of bacteroides, fusobacterium, ac ...19806249198
acetate kinase in the genus veillonella: effect of succinate, serological cross-reactivity, and separation by electrophoresis.acetate kinases from the genus veillonella were divided into two types: a succinate-stimulated enzyme and a succinate-independent enzyme. three strains, v. parvula atcc 17743 (antigenic group ii), v. parvula atcc 17744 (v), and v. parvula atcc 10790 (vi), contained the succinate-stimulated enzyme. among four types strains of v. alcalescens, three strains, atcc 17747 (i), atcc 17746 (iii), and atcc 17748 (vii), contained the succinate-independent enzyme, whereas only one strain, atcc 17745 (iv), ...19806154045
[the new medium for extensive survey of bacterial fibrinolytic activity (author's transl)]. 19806940631
prolonged primary incubation in the isolation of anaerobic bacteria from clinical specimens.the value of prolonged primary anaerobic incubation for the isolation of anaerobic bacteria from clinical material was assessed by comparison of two incubation systems. continuous incubation of primary anaerobic plates for 48 h was superior to conventional methods in that more anaerobes were isolated. the results indicate that in some cases prolonged incubation may be the only method of achieving the correct bacteriological diagnosis, particularly when relatively oxygen-sensitive strains are inv ...19806770094
endotoxins of gram-negative anaerobic bacteria.the author reviews the data available on endotoxins of fusobacterium, sphaerophorus, leptotrichia, and veillonella species, and reports his own findings with lipopolysaccharides extracted from bacteroides fragilis strains by the trichloroacetic acid method. the latter extracts showed a very low toxicity for mice after i.v. injection of dosages of less than or equal to 500 micrograms and a slight leucopenic effect in mice after intraperitoneal injection of 50 micrograms.19807005091
bone resorption stimulated by lipopolysaccharides from bacteroides, fusobacterium and veillonella, and by the lipid a and the polysaccharide part of fusobacterium lipopolysaccharide.lipopolysaccharides (lps) isolated from oral strains of veillonella, fusobacterium and bacteroides stimulated the release of 45ca from prelabeled fetal rat bones in culture. there was a typical dose-response relationship between the quantities of released 45ca and lps used for stimulation. bacteroides-lps proved to be the less active inducer of 45ca release. lps had no stimulating effect on the release of 45ca from devitalized bone. the stimulated 45ca release was paralleled by an increase in th ...19807017892
comparison of the predominant cultivable microflora in the dental plaque of macaca mulatta (rhesus) and macaca fascicularis (cynomolgus).the predominant cultivable bacteria in the supra-gingival plaque of old world monkeys were similar to those in human plaque. a significant interanimal species difference was observed in the absolute and relative concentration of specific microbial populations. streptococcus mutans serotype c was found primarily in rhesus plaque and serotype e in cynomolgus plaque.19806932418
[saprophytic and opportunistic non spore-forming anaerobic microflora of the vagina (author's transl)].a microbiological survey has been carried out on 179 healthy, child-bearing aged, non-pregnant women, with the aim to evaluate the incidence of anaerobic non-spore forming bacteria in the normal vaginal flora. this group of bacteria has been isolated in 50.3% of women, with a clear prevalence of "anaerobic streptococci " and bacteroides, followed by fusobacterium and veillonella. no propionibacterium, eubacterium or bifidobacterium have been isolated. according to many authors the non-spore form ...19807018415
[the distribution of obligate anaerobic bacteria in oral cavity (author's transl)]. 19806939343
acid formation by mixed cultures of cariogenic strains of streptococcus mutans and veillonella alcalescens. 19806940522
rapid presumptive identification of anaerobes in blood cultures by gas-liquid chromatography.production of volatile and nonvolatile metabolic acids in blood culture broths by aerobic, facultative anaerobic, and obligate anaerobic bacteria was analyzed by gas-liquid chromatography. anaerobic blood culture isolates were presumptively identified by the qualitative analysis of volatile fatty acids. isolates, with a characteristic gram stain reaction and cellular morphology, were identified by the following acid patterns: bacteriodes fragilis group with acetic and propionic acids; fusobacter ...19807381002
characterization of the adherence properties of streptococcus salivarius.the adherence and aggregation properties of 46 human oral streptococcus salivarius isolates were examined. a total of 41% of the isolates aggregated with whole human saliva, 50% aggregated with human erythrocytes, and 85% adhered to human buccal epithelial cells. strains that aggregated with saliva and erythrocytes usually reacted with streptococcus group k typing serum whereas the non-hemagglutinating strains did not. k+ strains also adhered more strongly to human buccal epithelial cells than k ...19807216420
selective antibacterial properties of lysozyme for oral microorganisms.the antibacterial properties of lysozyme were investigated with oral microorganisms representing the seven serotypes (a through g) of streptococcus mutans, veillonella alcalescens, and the virulent (v) and avirulent (av) strains of actinomyces viscosus t14. growth of bacteria in defined medium was monitored spectrophotometrically after the addition of various amounts (25 mug to 5 mg/ml) of enzyme. no growth inhibition of v. alcalescens was observed. inhibition of a. viscosus t14(v) and a. viscos ...19807216430
ct demonstration of septic thrombosis of the inferior vena cava. 19817217450
bacteria and gallstones. etiological significance.two hundred consecutive gallstone cases have been subjected to bacteriological study employing improved anaerobic culture techniques. in addition to clostridia species, species of anaerobes such as bacteroides fragilis, peptococcus, veillonella, and eubacterium are found to be present, some of which possessed beta-glucuronidase activity. this finding has a certain bearing on the etiology of bile pigment calcium stones. in addition to escherichia coli, bacteroides and clostridium often found in t ...19817238247
identification of a streptococcus salivarius cell wall component mediating coaggregation with veillonella alcalescens v1.cell walls of streptococcus salivarius hb aggregated veillonella alcalescens v1, but cell walls of the mutant s. salivarius hb-v5 did not. we found no correlation between the presence of fimbriae on streptococcal walls and the ability to aggregate veillonella strains. treatment of the walls with lysozyme solubilized a fraction which possessed veillonella-aggregating activity. solubilized cell wall preparations of strain hb contained three major (glyco)proteins as determined by sodium dodecyl sul ...19817251145
aerobic and anaerobic bacteria in tonsils of children with recurrent tonsillitis.tonsils were obtained from 50 children suffering from recurrent tonsillitis. patients' ages ranged from 2.5 to 17 years (mean 6 years); 29 were males and 21 females. the tonsils were sectioned in half after heat searing of the surface and the core material was cultured for aerobic and anaerobic microorganisms. mixed aerobic and anaerobic flora was obtained in all patients, yielding an average of 7.8 isolates (4.1 anaerobes and 3.7 aerobes) per specimen. there were 207 anaerobes isolated. the pre ...19817271131
role of interbacterial adherence in colonization of the oral cavities of gnotobiotic rats infected with streptococcus mutans and veillonella alcalescens.the role of interbacterial adherence in the colonization of the rate oral cavity was investigated with aggregating and nonaggregating strains of veillonella alcalescens and streptococcus mutans. v. alcalescens v-1 and s. mutans m-7 rapidly formed large stable aggregates when mixed in vitro. aggregates could be reduced in size by sonication, but they could not be completely dispersed, indicating that bonding between the organisms was strong. v. alcalescens v-1 did not coaggregate with s. mutans c ...19817275312
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