Publications

cultivable anaerobic microbiota of severe early childhood caries.	severe early childhood caries (ecc), while strongly associated with streptococcus mutans using selective detection (culture, pcr), has also been associated with a widely diverse microbiota using molecular cloning approaches. the aim of this study was to evaluate the microbiota of severe ecc using anaerobic culture. the microbial composition of dental plaque from 42 severe ecc children was compared with that of 40 caries-free children. bacterial samples were cultured anaerobically on blood and ac ...	2011	21289150
Early microbial succession in redeveloping dental biofilms in periodontal health and disease.	Teles FR, Teles RP, Uzel NG, Song XQ, Torresyap G, Socransky SS, Haffajee AD. Early microbial succession in redeveloping dental biofilms in periodontal health and disease. J Periodont Res 2011; doi: 10.1111/j.1600-0765.2011.01409.x. © 2011 John Wiley & Sons A/S Background and Objective: The development of dental biofilms after professional plaque removal is very rapid. However, it is not clear whether most bacterial species return at similar rates in periodontally healthy and periodontitis subj ...	2011	21895662
detection of nine microorganisms from the initial carious root lesions using a taqman-based real-time pcr.	oral diseases (2011) objective: the purpose of this study was to quantify nine selected cariogenic bacteria in plaque from sound root surfaces and initial carious root lesions using taqman pcr and to analyse a putative dependence on the kind of initial periodontal treatment. material and methods: fifty-four subjects with generalized chronic periodontitis were randomly allocated to one of the following initial periodontal therapies: full-mouth disinfection, full-mouth scaling and root planing o ...	2011	21605286
veillonella spondylodiscitis in a healthy 76-year-old lady.	purpose: to report a case of veillonella spondylodiscitis in a healthy 76-year-old lady. methods: a previously healthy 76-year-old lady presented with worsening axial back pain at the thoracolumbar junction, fever and loss of weight. examination revealed deep tenderness over the thoracolumbar junction with painful and restricted spinal movements. the lower limb motor power, sensation and reflexes were normal. results: radiographs of the lumbosacral spine showed evidence of spinal instability wit ...	2011	21674211
anaerobic bacteria colonizing the lower airways in lung cancer patients.	anaerobes comprise most of the endogenous oropharyngeal microflora, and can cause infections of airways in lung cancer patients who are at high risk for respiratory tract infections. the aim of this study was to determine the frequency and species diversity of anaerobes in specimens from the lower airways of lung cancer patients. sensitivity of the isolates to conventional antimicrobial agents used in anaerobe therapy was assessed. respiratory secretions obtained by bronchoscopy from 30 lung can ...	2011	21744326
pyrosequencing-based molecular monitoring of the intestinal bacterial colonization in preterm infants.	objectives:: to investigate the previously unexplored diversity of neonatal intestinal microbiota and monitor early intestinal colonization patterns in korean preterm infants using high-throughput pyrosequencing technology combined with 16s rdna- based molecular methods methods:: a total of 46,369 partial 16s rdna sequences obtained from 30 fecal samples serially taken from 10 very low birth weight preterm infants were analyzed. results:: a significant proportion of the molecular species (21.9%) ...	2011	21734604
genetic transformation of veillonella parvula.	veillonellae are one of the most prevalent and predominant microorganisms in both the supra- and subgingival plaques of the human oral cavity. veillonellae's mutualistic relationships with the early, middle, and late colonizers of the oral cavity make them an important component of oral biofilm ecology. unlike other ubiquitous early colonizers in the oral cavity, surprisingly little is known about veillonella biology due to our lack of ability to genetically transform this group of bacteria. the ...	2011	21707732
enteral tube feeding alters the oral indigenous microbiota in elderly adults.	enteral tube feeding is widely used to maintain nutrition for elderly adults with eating difficulties, but its long-term use alters the environment of the oral ecosystem. this study characterized the tongue microbiota of tube-fed elderly adults by analyzing the 16s rrna gene. the terminal restriction fragment length polymorphism (t-rflp) profiles of 44 tube-fed subjects were compared with those of 54 subjects fed orally (average age 86.4 -¦ 6.9 years). barcoded pyrosequencing data were also obta ...	2011	21821752
phenotypic identification of over 1000 isolates of anaerobic bacteria recovered between 1999 and 2008 in a major costa rican hospital.	because of limitations in infrastructure, the aetiology of infections caused by anaerobic bacteria is seldom determined in clinical laboratories of developing countries. this study reports on the identification of 1010 anaerobic bacterial isolates collected between 1999 and 2008 in a major costa rican hospital with the use of two commercial phenotypic systems (rapid 32a and api 20a). approximately 60% of the isolates were gram-positive and, among the 35 species of gram-positive bacteria found, t ...	2011	21722256
oral microbiome profiles: 16s rrna pyrosequencing and microarray assay comparison.	the human oral microbiome is potentially related to diverse health conditions and high-throughput technology provides the possibility of surveying microbial community structure at high resolution. we compared two oral microbiome survey methods: broad-based microbiome identification by 16s rrna gene sequencing and targeted characterization of microbes by custom dna microarray.	2011	21829515
target region selection is a critical determinant of community fingerprints generated by 16s pyrosequencing.	pyrosequencing of 16s rrna genes allows for in-depth characterization of complex microbial communities. although it is known that primer selection can influence the profile of a community generated by sequencing, the extent and severity of this bias on deep-sequencing methodologies is not well elucidated. we tested the hypothesis that the hypervariable region targeted for sequencing and primer degeneracy play important roles in influencing the composition of 16s pyrotag communities. subgingival ...	2011	21738596
Microbial community analysis and identification of alternative host-specific fecal indicators in fecal and river water samples using pyrosequencing.	It is important to know the comprehensive microbial communities of fecal pollution sources and receiving water bodies for microbial source tracking. Pyrosequencing targeting the V1-V3 hypervariable regions of the 16S rRNA gene was used to investigate the characteristics of bacterial and Bacteroidales communities in major fecal sources and river waters. Diversity analysis indicated that cow feces had the highest diversities in the bacterial and Bacteroidales group followed by the pig sample, with ...	2011	21887641
Preliminary characterization of the oral microbiota of Chinese adults with and without gingivitis.	ABSTRACT: BACKGROUND: Microbial communities inhabiting human mouth are associated with oral health and disease. Previous studies have indicated the general prevalence of adult gingivitis in China to be high. The aim of this study was to characterize in depth the oral microbiota of Chinese adults with or without gingivitis, by defining the microbial phylogenetic diversity and community-structure using highly paralleled pyrosequencing. METHODS: Six non-smoking Chinese, three with and three withou ...	2011	22152152
Cross-sectional and longitudinal comparisons of the predominant fecal microbiota compositions of a group of pediatric patients with cystic fibrosis and their healthy siblings.	Although only poorly documented, it can be assumed that intensive antibiotic treatments of chronic lung infections in patients with cystic fibrosis (CF) also affect the diversity and metabolic functioning of the gastrointestinal microbiota and potentially lead to a state of dysbiosis. A better knowledge of the differences in gut microbiota composition and stability between patients with CF and healthy subjects could lead to optimization of current antibiotic therapies and/or development of add-o ...	2011	21926193
profiling of dental plaque microflora on root caries lesions and the protein-denaturing activity of these bacteria.	to profile plaque microflora on root-caries lesions, and to examine the protein-denaturing activity as a pilot study.	2011	22165457
pyrosequencing-based analysis of the mucosal microbiota in healthy individuals reveals ubiquitous bacterial groups and micro-heterogeneity.	this study used 16s rrna-based pyrosequencing to examine the microbial community that is closely associated with the colonic mucosa of five healthy individuals. spatial heterogeneity in microbiota was measured at right colon, left colon and rectum, and between biopsy duplicates spaced 1 cm apart. the data demonstrate that mucosal-associated microbiota is comprised of firmicutes (50.9% ± 21.3%), bacteroidetes (40.2% ± 23.8%) and proteobacteria (8.6%± 4.7%), and that interindividual differences we ...	2011	21966408
evidence of a robust resident bacteriophage population revealed through analysis of the human salivary virome.	viruses are the most abundant known infectious agents on the planet and are significant drivers of diversity in a variety of ecosystems. although there have been numerous studies of viral communities, few have focused on viruses within the indigenous human microbiota. we analyzed 2 267 695 virome reads from viral particles and compared them with 263 516 bacterial 16s rrna gene sequences from the saliva of five healthy human subjects over a 2- to 3-month period, in order to improve our understand ...	2011	22158393
the subgingival microbiota of papillon-lefèvre syndrome.	background: there is little information about the microbiological profiles of periodontal lesions in papillon-lefèvre syndrome (pls) and the significance of bacteria in the pathogenesis of periodontitis in these patients. this comprehensive analysis of the subgingival microbiota in pls patients used 16s rrna clonal analysis and the 16s rrna-based human oral microbe identification microarray (homim). methods: thirteen pls patients, from 7 unrelated families, volunteered for this microbiological s ...	2011	22141356
the distribution and frequency of oral veillonella spp. in the tongue biofilm of healthy young adults.	five species of oral veillonella, v. atypica, v. denticariosi, v. dispar, v. parvula, and v. rogosae, have been suggested to be early colonizers of dental biofilm and causes of opportunistic infections and oral malodor. however, the pathogenicity and the distribution of oral veillonella spp. have not been clarified. previously, oral veillonella spp. were identified by using 16s rdna sequence analysis. in addition, recently, veillonella isolates from human tongue biofilm were identified by rpob g ...	2011	21850474
Changes in Human Fecal Microbiota Due to Chemotherapy Analyzed by TaqMan-PCR, 454 Sequencing and PCR-DGGE Fingerprinting.	We investigated whether chemotherapy with the presence or absence of antibiotics against different kinds of cancer changed the gastrointestinal microbiota.	2011	22194876
polymerase chain reaction-denaturing gradient gel electrophoresis analysis of bacterial community structure in the food, intestines, and feces of earthworms.	the bacterial communities in the food, intestines, and feces of earthworms were investigated by pcr-denaturing gradient gel electrophoresis (dgge). in this study, pcr-dgge was optimized by testing 6 universal primer sets for microbial 16s rrna in 6 pure culture strains of intestinal microbes in earthworms. one primer set effectively amplified 16s rrna from bacterial populations that were found in the food, intestines, and feces of earthworms. compared with the reference markers from the pure cul ...	2011	21887635
bacterial leakage along the implant-abutment interface: culture and dna checkerboard hybridization analyses.	objective: bacterial species have been found harboring the internal surface of dental implants as consequence of their failed connections. the aim of the present study was to compare the detection frequency of bacterial leakage from human saliva through the implant-abutment interface, under non-loading conditions, using either dna checkerboard or culture method. materials and methods: thirty dental implants with hexagonal platforms were connected to pre-machined abutments according to the manufa ...	2011	22092990
structure, viability and bacterial kinetics of an in vitro biofilm model using six bacteria from the subgingival microbiota.	there are few in vitro models available in the scientific literature for study of the structure, formation and development of the subgingival biofilm. the purpose of this study was to develop and validate an in vitro biofilm model, using representative selected bacteria from the subgingival microbiota.	2011	21261622
the effect of xylitol on the composition of the oral flora: a pilot study.	our aim was to investigate the effect of short-term xylitol consumption on the microbial composition of plaque and saliva.	2011	21311610
olsenella umbonata sp. nov., a microaerotolerant anaerobic lactic acid bacterium from the sheep rumen and pig jejunum, and emended descriptions of olsenella, olsenella uli and olsenella profusa.	strain a2 is an anaerobic, variably gram-stain-positive, non-spore-forming, small and irregularly rod-shaped bacterium from the ruminal fluid of a sheep that has been described informally as a representative of 'olsenella (basonym atopobium) oviles'. three phenotypically similar bacterial strains (lac15, lac16 and lac31(t)) were isolated in concert with veillonella magna lac18(t) from the mucosal jejunum of a pig. a phylogenetic analysis based on 16s rrna gene sequences revealed that strains a2, ...	2011	20435744
microbial shifts during dental biofilm re-development in the absence of oral hygiene in periodontal health and disease.	uzel ng, teles fr, teles rp, song xq, torresyap g, socransky ss, haffajee ad. microbial shifts during dental biofilm re-development in the absence of oral hygiene in periodontal health and disease. j clin peridontol 2011; doi: 10.1111/j.1600-051x.2011.01730.x. abstract: aim: to monitor microbial shifts during dental biofilm re-development. materials and methods: supra- and subgingival plaque samples were taken separately from 28 teeth in 38 healthy and 17 periodontitis subjects at baseline and i ...	2011	21488936
activity of garenoxacin against 536 unusual anaerobes including 128 recovered from acute pelvic infections.	garenoxacin, a des-(f)6-quinolone, was tested using the agar dilution method against 536 anaerobic bacteria, composed of 408 unusual strains from various sources and 128 pelvic isolates. only 33/408 (8%) unusual isolates and 6/128 (4.7%) pelvic isolates had garenoxacin mics ôëñ 4 ++g/ml, and 18 and 3 (4.4% and 2.3% of the respective totals) had mics ôëñ 8 ++g/ml. less susceptible unusual isolates included 7/15 veillonella sp. from various clinical sources, 6/14 fusobacterium varium from predomin ...	2011	21398076
clustering of subgingival microbial species in adolescents with periodontitis.	it has become increasingly recognized that groups of microorganisms interact within the subgingival plaque of adult subjects with periodontitis. it is much less clear, however, whether the consortia of microorganisms associated with periodontitis are different in early and more advanced cases of periodontitis. to investigate this point further, subgingival plaque was collected from six sites in 87 adolescents with periodontitis and 73 controls and the samples were analyzed for the detection of 1 ...	2011	21410554
molecular detection of in-vivo microbial contamination of metallic orthodontic brackets by checkerboard dna-dna hybridization.	knowing the microbiota that colonizes orthodontic appliances is important for planning strategies and implementing specific preventive measures during treatment. the purpose of this clinical trial was to evaluate in vivo the contamination of metallic orthodontic brackets with 40 dna probes for different bacterial species by using the checkerboard dna-dna hybridization (cddh) technique.	2012	22196182
microbial profile of patients with periodontitis compared with healthy subjects.	objective: to define and compare the microbiologic profile of subgingival plaque in german patients with periodontitis (including aggressive and advanced chronic periodontitis) and healthy subjects and to determine significant association between isolates and clinical status. aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, and tannerella forsythia are major periodontal pathogens, though it is recognized that other species may also contribute to the pathogenesis of periodontal di ...	2012	22257880