Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| veillonella rogosae sp. nov., an anaerobic, gram-negative coccus isolated from dental plaque. | strains of a novel anaerobic, gram-negative coccus were isolated from the supra-gingival plaque of children. independent strains from each of six subjects were shown, at a phenotypic level and based on 16s rrna gene sequencing, to be members of the genus veillonella. analysis revealed that the six strains shared 99.7 % similarity in their 16s rrna gene sequences and 99.0 % similarity in their rpob gene sequences. the six novel strains formed a distinct group and could be clearly separated from r ... | 2008 | 18319459 |
| molecular identification of bacteria on the tongue dorsum of subjects with and without halitosis. | compare the microbial profiles on the tongue dorsum in patients with halitosis and control subjects in a uk population using culture-independent techniques. | 2008 | 18336372 |
| salivary microbiota of hiv-positive children and its correlation with hiv status, oral diseases, and total secretory iga. | this study aimed to investigate the prevalence and levels of salivary microorganisms in hiv-positive children, and their correlation to hiv status, oral lesions, and salivary iga levels. | 2008 | 18384349 |
| bacterial profiles of root caries in elderly patients. | culture-based studies have shown that streptococcus mutans and lactobacilli are associated with root caries (rc). the purpose of the present study was to assess the bacterial diversity of rc in elderly patients by use of culture-independent molecular techniques and to determine the associations of specific bacterial species or bacterial communities with healthy and carious roots. plaque was collected from root surfaces of 10 control subjects with no rc and from 11 subjects with rc. the bacterial ... | 2008 | 18385433 |
| bleeding on probing differentially relates to bacterial profiles: the oral infections and vascular disease epidemiology study. | various bacterial species are differentially prevalent in periodontal health, gingivitis or periodontitis. we tested the independent associations between three bacterial groupings and gingival inflammation in an epidemiological study. | 2008 | 18400025 |
| effect of veillonella parvula on the antimicrobial resistance and gene expression of streptococcus mutans grown in a dual-species biofilm. | our previous studies showed that streptococcus mutans and veillonella parvula dual-species biofilms have a different acid production profile and a higher resistance to chlorhexidine than their single-species counterparts. the aim of the current study was to test whether the susceptibility of s. mutans grown in the presence of v. parvula is also decreased when it is exposed to various other antimicrobials. furthermore, the aim was to identify other changes in the physiology of s. mutans when v. p ... | 2008 | 18402603 |
| microbiological evaluation of primary endodontic infections in teeth with and without sinus tract. | to examine the microbiological status of primary endodontic infections in teeth with and without a sinus tract. | 2008 | 18422583 |
| diverse and novel oral bacterial species in blood following dental procedures. | we identified oral bacterial species in blood cultures following single-tooth extraction and tooth brushing. sequence analysis of 16s rrna genes identified 98 different bacterial species recovered from 151 bacteremic subjects. of interest, 48 of the isolates represented 19 novel species of prevotella, fusobacterium, streptococcus, actinomyces, capnocytophaga, selenomonas, and veillonella. | 2008 | 18434561 |
| a microbiological profile of symptomatic teeth with primary endodontic infections. | the aim of this study was to evaluate the composition of the microbiota of primary endodontic infections associated with symptomatic teeth. samples were collected by means of a #15 h-type file and 2 sterile paper points from 60 symptomatic (n = 30) or asymptomatic (n = 30) single-rooted teeth with necrotic pulp. the presence of 40 bacterial species was determined by the checkerboard dna-dna hybridization method. the species found in higher counts (x10(5)) in symptomatic cases were fusobacterium ... | 2008 | 18436031 |
| predominant bacteria recovered from a periodontitis site in a hamster model raised by silk-ligature with porphyromonas gingivalis infection. | we isolated oral bacteria that coexisted with porphyromonas gingivalis in a hamster periodontitis model. as predominant bacteria in the periodontitis site, collinsella-reltaed strains, eubacterium-reltaed strains, streptococcus suis-related strains, and veillonella parvula-reltaed strains were detected. in addition, actinomyces, bacteroides, and p. gingivalis were also isolated predominantly. the results suggest that the bacterial composition of the periodontitis site in hamsters is complex, as ... | 2008 | 18460794 |
| [monitoring of antibiotic resistance of gram negative anaerobes]. | using an agar reference method (norma m11-a5, national committee for clinical and laboratory standards) the minimal inhibitory concentrations of nine antibiotics were determined for 376 anaerobic strains. the following strains were investigated: 254 bacteroides fragilis group (including 143 b. fragilis), 122 other gram-negative anaerobes (bacteroides spp., prevotella, fusobacterium, porphyromonas, suterella, desulfomonas, veillonella). | 2008 | 18482812 |
| intestinal mucosa-associated microflora in ulcerative colitis patients before and after restorative proctocolectomy with an ileoanal pouch. | this study was designed to identify the mucosa-associated microflora in patients with severe ulcerative colitis before and after restorative proctocolectomy with ileoanal pouch construction in comparison with historic controls. | 2008 | 18483827 |
| bacteria of dental caries in primary and permanent teeth in children and young adults. | although streptococcus mutans has been implicated as a major etiological agent of dental caries, our cross-sectional preliminary study indicated that 10% of subjects with rampant caries in permanent teeth do not have detectable levels of s. mutans. our aims were to use molecular methods to detect all bacterial species associated with caries in primary and permanent teeth and to determine the bacterial profiles associated with different disease states. plaque was collected from 39 healthy control ... | 2008 | 18216213 |
| detection of anaerobic bacteria in high numbers in sputum from patients with cystic fibrosis. | pulmonary infection in cystic fibrosis (cf) is polymicrobial and it is possible that anaerobic bacteria, not detected by routine aerobic culture methods, reside within infected anaerobic airway mucus. | 2008 | 18263800 |
| veillonella as a cause of chronic anaerobic pneumonitis. | anaerobes are not well recognized as a cause of chronic respiratory infections. a 44-year-old man was referred for evaluation of a progressive pulmonary disease of 7-month duration characterized by hemoptysis and fever. for these complaints, based on the radiological picture, he had already received antituberculous therapy without any relief. he was also subjected to bronchial artery embolization prior to referral. evaluation of the patient led to a diagnosis of chronic anaerobic pneumonitis. an ... | 2008 | 18547858 |
| influence of local tetracycline on the microbiota of alveolar osteitis in rats. | the aim of the present study was to evaluate the effects of local tetracycline on the occurrence of alveolar osteitis in rats, and on the microbiota associated to this infection. forty wistar rats were randomly assigned to 4 groups (n=10): i - the rats had the maxillary right incisor extracted and the alveolar wound did not receive any treatment; ii - adrenaline and ringer-pras were introduced into the alveolar wound; iii - the alveolar wound was irrigated with sterile saline; and iv - the alveo ... | 2008 | 18568225 |
| the predominant cultivable veillonella spp. of the tongue of healthy adults identified using rpob sequencing. | the predominant veillonella spp. were isolated from the dorsum surface of the tongues of 11 healthy adults and identified to species level using rpob sequencing because 16s ribosomal rna sequence analysis does not reliably differentiate between all members of this genus. in all, 253 isolates were identified and the mean proportion (+/- se) of veillonella spp. per sample was 16.2 (+/- 3.6) with a range of 3.0% to 36.3% of the total anaerobic colony count. the predominant species were veillonella ... | 2008 | 18582335 |
| periodontal status of patients with dentin dysplasia type i: report of three cases within a family. | dentin dysplasia type i (ddi) is a rare hereditary disturbance of dentin formation. it is characterized by clinically normal-appearing crowns; obliteration of pulp chambers; and short, blunted and malformed roots that are commonly associated with periodontal attachment loss (pal). in this context, we report three cases within a family with similar clinical and radiographic features of ddi but with differing microbiologic and periodontal conditions. | 2008 | 18597615 |
| necessary corrections to the approved lists of bacterial names according to rule 40d (formerly rule 46). opinion 86. | the judicial commission affirms that, according to rule 40d, formerly rule 46, of the bacteriological code, the authorship of a number of subspecies names included on the approved lists of bacterial names must be corrected. these names are acetobacter aceti subsp. aceti, acetobacter pasteurianus subsp. pasteurianus, bacteroides melaninogenicus subsp. melaninogenicus, campylobacter fetus subsp. fetus, mycobacterium chelonae subsp. chelonae, propionibacterium freudenreichii subsp. freudenreichii, ... | 2008 | 18676492 |
| biofilm microbial communities of denture stomatitis. | denture stomatitis is a common lesion that affects denture wearers. its multifactorial etiology seems to depend on a complex and poorly characterized biofilm. the purpose of this study was to assess the composition of the microbial biofilm obtained from complete denture wearers with and without denture stomatitis using culture-independent methods. | 2008 | 18793366 |
| characterization of a streptococcus sp.-veillonella sp. community micromanipulated from dental plaque. | streptococci and veillonellae occur in mixed-species colonies during formation of early dental plaque. one factor hypothesized to be important in assembly of these initial communities is coaggregation (cell-cell recognition by genetically distinct bacteria). intrageneric coaggregation of streptococci occurs when a lectin-like adhesin on one streptococcal species recognizes a receptor polysaccharide (rps) on the partner species. veillonellae also coaggregate with streptococci. these genera intera ... | 2008 | 18805978 |
| [influence of the fluoride releasing dental materials on the bacterial flora of dental plaque]. | the assessment of influence of silver-free, fluor releasing dental materials on dental plaque bacteria quantity. 17 patients were included into the study. 51 restorations were placed following manufacturers recommendations. following materials were used: conventional glassionomer ketac-molar espe, resin modified glassionomer fuji ii lc gc and fluor containing composite charisma heraeus kulzer class v restorations were placed in following teeth of upper and lower jaw: canines, first bicuspids, se ... | 2008 | 18819449 |
| mlpa diagnostics of complex microbial communities: relative quantification of bacterial species in oral biofilms. | a multitude of molecular methods are currently used for identification and characterization of oral biofilms or for community profiling. however, multiplex pcr techniques that are able to routinely identify several species in a single assay are not available. multiplex ligation-dependent probe amplification (mlpa) identifies up to 45 unique fragments in a single tube pcr. here we report a novel use of mlpa in the relative quantification of targeted microorganisms in a community of oral microbiot ... | 2008 | 18824042 |
| [antibiotic therapy in odontogenic infections in children and adolescents: pharmacokinetic/pharmacodynamic analysis]. | the objective of this study was to evaluate the efficacy of the most commonly used antimicrobial treatments in odontogenic infections in children and adolescents on the basis of pharmacokinetic/ pharmacodynamic (pk/pd) criteria. | 2008 | 19100192 |
| veillonella magna sp. nov., isolated from the jejunal mucosa of a healthy pig, and emended description of veillonella ratti. | a bacterium, designated strain lac18(t), was isolated in pure culture from the mucosal jejunum of a healthy pig, using a medium selective for anaerobic lactic acid bacteria and containing porcine gastric mucin as the main carbon and nitrogen source. cells of this strain were coccus-shaped, arranged singly or in pairs and were gram-stain-negative, oxidase-negative, non-spore-forming, anaerobic and microaerotolerant. an analysis based on 16s rrna gene sequences indicated that strain lac18(t) shoul ... | 2008 | 19060053 |
| commensal intestinal bacterial strains trigger ankylosing enthesopathy of the ankle in inbred b10.br (h-2(k)) male mice. | joint disease ankylosing enthesopathy (ankent) naturally occurs in inbred mice with c57bl/10 genetic background. ankent has many parallels to human ankylosing spondylitis (as) and represents an animal model for as. environmental conditions (i.e., microbial load of the organism) are among the risk factors for ankent, similar to as. the role of microflora in the development of ankent was investigated. ankent was tested in four experimental groups of germ-free mice associated with different numbers ... | 2008 | 18840492 |
| [oral cavity microbiocenosis in healthy adolescents and patients with chronic gastroduodenitis]. | to determine characteristics of microbial associations and degree of oral cavity colonization by different biotopes in healthy adolescents and patients with chronic gastroduodenitis. | 2008 | 19186547 |
| microbial manufacture of chalcogenide-based nanoparticles via the reduction of selenite using veillonella atypica: an in situ exafs study. | the ability of metal-reducing bacteria to produce nanoparticles, and their precursors, can be harnessed for the biological manufacture of fluorescent, semiconducting nanomaterials. the anaerobic bacterium veillonella atypica can reduce selenium oxyanions to form nanospheres of elemental selenium. these selenium nanospheres are then further reduced by the bacterium to form reactive selenide which could be precipitated with a suitable metal cation to produce nanoscale chalcogenide precipitates, su ... | 2008 | 21825617 |
| distribution of selected cariogenic bacteria in five different intra-oral habitats in young children. | knowledge of the colonization patterns and composition of the oral microbiota can lead to a better understanding of disease initiation. | 2009 | 19207737 |
| does pregnancy have an impact on the subgingival microbiota? | we investigated clinical and subgingival microbiologic changes during pregnancy in 20 consecutive pregnant women > or =18 years not receiving dental care. | 2009 | 19228092 |
| gastric acid reduction leads to an alteration in lower intestinal microflora. | to clarify the alterations in lower intestinal microflora induced by gastric acid reduction, the dynamics of 12 major genera or groups of bacteria comprising the microflora in feces and colonic contents were examined by quantitative real-time pcr in proton pump inhibitor-treated rats and in asymptomatic human subjects with hypochlorhydria. in both rat and human experiments, most genera or groups of intestinal microflora (facultative and obligate anaerobes) proliferated by gastric acid reduction, ... | 2009 | 19248769 |
| molecular characterization of the stomach microbiota in patients with gastric cancer and in controls. | persistent infection of the gastric mucosa by helicobacter pylori can initiate an inflammatory cascade that progresses into atrophic gastritis, a condition associated with reduced capacity for secretion of gastric acid and an increased risk of developing gastric cancer. the role of h. pylori as an initiator of inflammation is evident but the mechanism for development into gastric cancer has not yet been proven. a reduced capacity for gastric acid secretion allows survival and proliferation of ot ... | 2009 | 19273648 |
| a review of dental implants and infection. | dental implants have become increasingly common for the management of tooth loss. despite their placement in a contaminated surgical field, success rates are relatively high. this article reviews dental implants and highlights factors leading to infection and potential implant failure. a literature search identified studies analysing the microbial composition of peri-implant infections. the microflora of dental peri-implantitis resembles that found in chronic periodontitis, featuring predominant ... | 2009 | 19329223 |
| characterization of the complex bacterial communities colonizing biliary stents reveals a host-dependent diversity. | this study provides a comprehensive survey of the spatial and temporal bacterial composition of biliary stent biofilms. the bacterial diversity, distribution and dynamics of 59 biliary and 4 pancreatic stent communities from 40 patients being treated at two different hospitals, which implant stents either simultaneously or consecutively, were characterized by single-strand conformation polymorphism (sscp) analysis. fifty-one phylotypes belonging to 5 bacterial phyla and 24 bacterial families wer ... | 2009 | 19360025 |
| diversity and site-specificity of the oral microflora in the elderly. | the purpose of the present study was to describe the bacterial diversity in the oral cavity of the elderly without root caries using bacterial microarrays, and to determine the site- and subject-specificity of bacterial colonization. samples were collected from the tongue dorsum, mucosa of the buccal fold, hard palate, supragingival plaque from sound root surfaces, and subgingival plaque from the same roots. a new 16 s rrna gene-based microarray method was used for the simultaneous detection of ... | 2009 | 19373498 |
| citric acid wastewater as electron donor for biological sulfate reduction. | citrate-containing wastewater is used as electron donor for sulfate reduction in a biological treatment plant for the removal of sulfate. the pathway of citrate conversion coupled to sulfate reduction and the microorganisms involved were investigated. citrate was not a direct electron donor for the sulfate-reducing bacteria. instead, citrate was fermented to mainly acetate and formate. these fermentation products served as electron donors for the sulfate-reducing bacteria. sulfate reduction acti ... | 2009 | 19399495 |
| multi-species biofilm formation on dental materials and an adhesive patch. | this laboratory study assessed the influence of surface roughness and contact time on the formation of a multi-species biofilm on dental materials (adhesive patch, composite, amalgam and enamel). | 2009 | 19408815 |
| [changes in oral microflora in patients with recurrent oral ulcers]. | to investigate the correlation between the changes in oral microflora and recurrent oral ulcers (rou). | 2009 | 19460727 |
| [oral cavity microbiocenose in healthy teenagers and patients with chronic gastritis and gastroduodenitis]. | microbial association distinctive features and colonization degree of different oral cavity biotope were established in healthy teenagers and patients with chronic gastritis and gastroduodenitis. there were studied mucous membrane surfaces of cheek, tongue, content of gingival crevice, oral fluid and dental plaque of 24 healthy persons of the age 12-17 years and 29 patients of the same age with gastritis and gastroduodenitis. on the mucous membranes of the ill teenagers there were found porphiro ... | 2009 | 19491778 |
| bacterial colonization of enamel in situ investigated using fluorescence in situ hybridization. | oral biofilms are one of the greatest challenges in dental research. the present study aimed to investigate initial bacterial colonization of enamel surfaces in situ using fluorescence in situ hybridization (fish) over a 12 h period. for this purpose, bovine enamel slabs were fixed on buccal sites of individual splints worn by six subjects for 2, 6 and 12 h to allow biofilm formation. specimens were processed for fish and evaluated with confocal laser-scanning microscopy, using probes for eubact ... | 2009 | 19528150 |
| interspecies signaling between veillonella atypica and streptococcus gordonii requires the transcription factor ccpa. | streptococcus gordonii and veillonella atypica, two early-colonizing members of the dental plaque biofilm, participate in a relationship that results in increased transcription of the s. gordonii gene amyb, encoding an alpha-amylase. we show that the transcription factor ccpa is required for this interspecies interaction. | 2009 | 19542285 |
| aggregatibacter actinomycetemcomitans builds mutualistic biofilm communities with fusobacterium nucleatum and veillonella species in saliva. | human oral bacterial pathogens grow in attached multispecies biofilm communities. unattached cells are quickly removed by swallowing. therefore, surface attachment is essential for growth, and we investigated multispecies community interactions resulting in mutualistic growth on saliva as the sole nutritional source. we used two model systems, saliva-coated transferable solid-phase polystyrene pegs (peg biofilms) and flow cells with saliva-coated glass surfaces. fluorescent antibody staining and ... | 2009 | 19564387 |
| identification of oral species of the genus veillonella by polymerase chain reaction. | members of the genus veillonella cannot be reliably distinguished by their biochemical characteristics and phenotypic features. moreover, dna-dna hybridization and sequence analyses of the 16s ribosomal rna gene including random fragment length polymorphism analysis, are complex and time-consuming procedures that are not well-suited to identifying oral species of veillonella: veillonella atypica, veillonella denticariosi, veillonella dispar, veillonella parvula, and veillonella rogosae. | 2009 | 19572893 |
| granulocyte chemotactic protein 2 (gcp-2/cxcl6) complements interleukin-8 in periodontal disease. | mucosal inflammatory responses are orchestrated largely by pro-inflammatory chemokines. the chemokine granulocyte chemotactic protein 2 (cxcl6) is involved in neutrophil recruitment and migration. previous studies have shown that granulocyte chemotactic protein 2 is up-regulated during mucosal inflammation (e.g. in inflammatory bowel disease), similarly to the functionally and structurally related chemokine interleukin-8. nevertheless, unlike interleukin-8, a role of granulocyte chemotactic prot ... | 2009 | 18842116 |
| factors affecting human supragingival biofilm composition. ii. tooth position. | little is known regarding the factors that affect the microbial composition of supragingival biofilms. this study was designed to test the hypothesis that tooth location affects the microbial composition of supragingival plaque beyond the effect due to plaque mass as reflected by total dna probe count. | 2009 | 18973539 |
| factors affecting human supragingival biofilm composition. i. plaque mass. | little is known about the factors that affect the microbial composition of supragingival biofilms. this study was designed to examine the relationship between total dna probe counts of supragingival biofilm samples, clinical parameters and supragingival biofilm composition. | 2009 | 18973540 |
| tinidazole inhibitory and cidal activity against anaerobic periodontal pathogens. | the in vitro activity of tinidazole against anaerobic periodontal pathogens (25 prevotella buccae, 18 prevotella denticola, 10 prevotella intermedia, 6 prevotella melaninogenica, 5 prevotella oralis, 10 fusobacterium nucleatum and 8 veillonella spp.) was determined by agar dilution. mic(90) values (minimum inhibitory concentration for 90% of the organisms) were 8 microg/ml for veillonella spp., 4 microg/ml for p. intermedia, 2 microg/ml for p. buccae, 1 microg/ml for fusobacterium spp. and 0.5 m ... | 2009 | 19097755 |
| the ecological proportion of indigenous bacterial populations in saliva is correlated with oral health status. | to obtain deeper insights into the etiology of oral disease, an understanding of the composition of the surrounding bacterial environments that lead to health or disease is required, which is attracting increasing attention. in this study, the bacterial compositions in the saliva of 200 subjects aged 15-40 years were depicted as peak patterns by terminal restriction fragment length polymorphism (t-rflp) analysis of 16s rrna genes. the subjects were classified into three clusters by partitioning ... | 2009 | 18830275 |
| quantitative variations in the vaginal bacterial population associated with asymptomatic infections: a real-time polymerase chain reaction study. | the real-time polymerase chain reaction (pcr) quantification of several vaginal bacterial groups in healthy women and patients developing asymptomatic bacterial vaginosis (bv) and candidiasis (ca) was performed. statistical analysis revealed that the bv condition is characterised by a great variability among subjects and that it is associated with a significant increase of prevotella, atopobium, veillonella and gardnerella vaginalis, and a drop in lactobacillus. on the contrary, the vaginal micr ... | 2009 | 18762999 |
| assessment of intraradicular bacterial composition by terminal restriction fragment length polymorphism analysis. | the aim of the study was to assess the bacterial community structures associated with endodontic infections using terminal restriction fragment length polymorphism (t-rflp), and to investigate the correlation of whole community profiles with the manifestation of particular clinical features. | 2009 | 19702949 |
| comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray. | this study compared the subgingival microbiota of subjects with refractory periodontitis (rp) to those in subjects with treatable periodontitis (grs = good responders) or periodontal health (ph) using the human oral microbe identification microarray (homim). | 2009 | 19722792 |
| mutualistic biofilm communities develop with porphyromonas gingivalis and initial, early, and late colonizers of enamel. | porphyromonas gingivalis is present in dental plaque as early as 4 h after tooth cleaning, but it is also associated with periodontal disease, a late-developing event in the microbial successions that characterize daily plaque development. we report here that p. gingivalis atcc 33277 is remarkable in its ability to interact with a variety of initial, early, middle, and late colonizers growing solely on saliva. integration of p. gingivalis into multispecies communities was investigated by using t ... | 2009 | 19749049 |
| proximal small intestinal microbiota and identification of rod-shaped bacteria associated with childhood celiac disease. | alterations in the composition of the microbiota in the intestine may promote development of celiac disease (cd). using scanning electron microscopy (sem) we previously demonstrated that rod-shaped bacteria were present on the epithelium of proximal small intestine in children with cd but not in controls. in this study we characterize the microbiota of proximal small intestine in children with cd and controls and identify cd-associated rod-shaped bacteria. | 2009 | 19755974 |
| treatment of irritable bowel syndrome with probiotics. an etiopathogenic approach at last? | irritable bowel syndrome (ibs) is the most common functional digestive disorder, and may affect 11-20% of the adult population in industrialized countries. in accordance with rome iii criteria (2006) ibs involves abdominal pain and bowel habit disturbance, which are not explained by structural or biochemical abnormalities. several hypotheses attempt to account for the pathophysiology of ibs, but the etiology still remains uncertain or obscure, perhaps multifactorial. abnormalities in colonic mic ... | 2009 | 19785495 |
| receptor recognition of and immune intracellular pathways for veillonella parvula lipopolysaccharide. | veillonella parvula is an anaerobic gram-negative coccus that is part of the normal flora of the animal and human mouth and gastrointestinal and genitourinary tracts. oral v. parvula is involved in the development of early periodontal disease as well as different types of serious infections. present data on molecular mechanisms responsible for innate immune response against veillonella are very scanty. the aim of this study was to investigate the toll-like receptor (tlr) pathways responsible for ... | 2009 | 19828771 |
| investigating different mechanisms for biogenic selenite transformations: geobacter sulfurreducens, shewanella oneidensis and veillonella atypica. | the metal-reducing bacteria geobacter sulfurreducens, shewanella oneidensis and veillonella atypica, use different mechanisms to transform toxic, bioavailable sodium selenite to less toxic, non-mobile elemental selenium and then to selenide in anaerobic environments, offering the potential for in situ and ex situ bioremediation of contaminated soils, sediments, industrial effluents, and agricultural drainage waters. the products of these reductive transformations depend on both the organism invo ... | 2009 | 19950474 |
| effect of herbal, essential oil, and chlorhexidine mouthrinses on the composition of the subgingival microbiota and clinical periodontal parameters. | the purpose of the present investigation was to determine if antimicrobial mouthrinses with different formulations could affect the composition of the subgingival microbiota and clinical parameters of adjacent tissues in periodontal maintenance subjects. | 2009 | 20128317 |
| identification of clinically important anaerobic bacteria by an oligonucleotide array. | anaerobic bacteria can cause a wide variety of infections, and some of these infections can be serious. conventional identification methods based on biochemical tests are often lengthy and can produce inconclusive results. an oligonucleotide array based on the 16s-23s rrna intergenic spacer (its) sequences was developed to identify 28 species of anaerobic bacteria and veillonella. the method consisted of pcr amplification of the its regions with universal primers, followed by hybridization of th ... | 2010 | 20129959 |
| diversity of veillonella spp. from subgingival plaque by polyphasic approach. | in a biofilm such as the subgingival microflora, strain-specific properties or factors induced by the host may impart a survival advantage to some bacterial strains. periodontal disease has been associated with chronic obstructive pulmonary disease (copd) and we previously found high amounts of veillonella in the subgingival microflora of copd subjects. differentiation of veillonella is difficult. the aims of this study were to identify subgingival veillonella isolates by phenotypic, genetic typ ... | 2010 | 20132189 |
| central role of the early colonizer veillonella sp. in establishing multispecies biofilm communities with initial, middle, and late colonizers of enamel. | human dental biofilm communities comprise several species, which can interact cooperatively or competitively. bacterial interactions influence biofilm formation, metabolic changes, and physiological function of the community. lactic acid, a common metabolite of oral bacteria, was measured in the flow cell effluent of one-, two- and three-species communities growing on saliva as the sole nutritional source. we investigated single-species and multispecies colonization by using known initial, early ... | 2010 | 20154130 |
| the use of molecular techniques for bacterial detection in the analysis of gastric aspirates collected from infants on the first day of life. | prospective service evaluation of the utility of molecular methods to analyse neonatal gastric aspirate specimens in a single neonatal unit and associated maternity unit. 43 newborn infants investigated for sepsis with median gestational age of 39 weeks (range 31-41 weeks) and median birth weight 3050 grams (range 1250-4220 g). gastric aspirates routinely collected within 12h of birth were analysed using conventional and molecular methods for bacterial detection, bacterial dna load and sequencin ... | 2010 | 20223606 |
| microbial equol production attenuates colonic methanogenesis and sulphidogenesis in vitro. | hydrogen gas produced during colonic fermentation is excreted in breath and flatus, or removed by hydrogen-consuming bacteria such as methanogens and sulphate-reducing bacteria. however, recent research has shown that h2 is also consumed by equol-producing bacteria during the reduction of daidzein into equol. in this study, the interactions between methanogens, sulphate-reducing, and equol-producing bacteria were investigated under in vitro simulated intestinal conditions. in the presence of dai ... | 2010 | 20298796 |
| transfer of antibiotic resistance by transformation with edna within oral biofilms. | we demonstrate that live donor veillonella dispar cells can transfer the conjugative transposon tn916 to four different streptococcus spp. recipients in a multispecies oral consortium growing as a biofilm in a constant depth film fermentor. additionally, we demonstrate that purified v. dispar dna can transform streptococcus mitis to tetracycline resistance in this experimental system. these data show that transfer of conjugative transposon-encoded antibiotic resistance can occur by transformatio ... | 2010 | 20337719 |
| consumption of human milk oligosaccharides by gut-related microbes. | human milk contains large amounts of complex oligosaccharides that putatively modulate the intestinal microbiota of breast-fed infants by acting as decoy binding sites for pathogens and as prebiotics for enrichment of beneficial bacteria. several bifidobacterial species have been shown to grow well on human milk oligosaccharides. however, few data exist on other bacterial species. this work examined 16 bacterial strains belonging to 10 different genera for growth on human milk oligosaccharides. ... | 2010 | 20394371 |
| response of subgingival bacteria to smoking cessation. | it has been demonstrated that smoking cessation alters the subgingival microbial profile; however, the response of individual bacteria within this ecosystem has not been well studied. the aim of this investigation, therefore, was to longitudinally examine the effect of smoking cessation on the prevalence and levels of selected subgingival bacteria using molecular approaches for bacterial identification and enumeration. subgingival plaque was collected from 22 smokers at the baseline and 12 month ... | 2010 | 20410352 |
| intragenomic and intraspecific heterogeneity in rrs may surpass interspecific variability in a natural population of veillonella. | as well as intraspecific heterogeneity, intragenomic heterogeneity between 16s rrna gene copies has been described for a range of bacteria. due to the wide use of 16s rrna gene sequence analysis for taxonomy, identification and metagenomics, evaluating the extent of these heterogeneities in natural populations is an essential prerequisite. we investigated inter- and intragenomic 16s rrna gene heterogeneity of the variable region v3 in a population of 149 clinical isolates of veillonella spp. of ... | 2010 | 20413553 |
| altered intestinal microbiota in irritable bowel syndrome. | recent studies have suggested that alterations in the composition of the intestinal microbiota may play an important role in irritable bowel syndrome (ibs) symptoms. however, an association between the composition of the intestinal microbiota and ibs symptoms has not been clearly demonstrated. in the current issue of the journal, tana et al. suggest that altered intestinal microbiota contributes to the symptoms of ibs through increased levels of organic acids. in fecal samples, ibs patients had ... | 2010 | 20414959 |
| mechanical non-surgical treatment of peri-implantitis: a single-blinded randomized longitudinal clinical study. ii. microbiological results. | peri-implantitis is common in patients with dental implants. we performed a single-blinded longitudinal randomized study to assess the effects of mechanical debridement on the peri-implant microbiota in peri-implantitis lesions. | 2010 | 20507380 |
| analysis of oral microbiota in children with dental caries by pcr-dgge and barcoded pyrosequencing. | oral microbiota plays a vital role in maintaining the homeostasis of oral cavity. dental caries are among the most common oral diseases in children and pathogenic bacteria contribute to the development of the disease. however, the overall structure of bacterial communities in the oral cavity from children with dental caries has not been explored deeply heretofore. we used high-throughput barcoded pyrosequencing and pcr-denaturing gradient gel electrophoresis (dgge) to examine bacterial diversity ... | 2010 | 20614117 |
| high temporal and inter-individual variation detected in the human ileal microbiota. | the diversity and temporal stability of the predominant bacteria in the human ileum was studied with the use of ileal effluent samples of seven individuals with brooke ileostomies. the total number of bacteria within the ileal effluent was in the range of 10⁷ -10⁸ bacteria per gram (wet weight). the diversity of the bacteria in the ileal effluent showed marked differences compared with that in faecal samples from age-matched healthy adults. the ileal effluent had a higher relative abundance of s ... | 2010 | 20626454 |
| necrotizing myositis of the deltoid following intramuscular injection of anabolic steroid. | 2010 | 20638885 | |
| suppression of human immunodeficiency virus type 1 replication in macrophages by commensal bacteria preferentially stimulating toll-like receptor 4. | protection from primary human immunodeficiency virus type 1 (hiv-1) infection has not yet been accomplished by vaccines inducing hiv-1-specific acquired immunity. nevertheless, it has been reported that a small subgroup of women remain resistant to hiv-1 infection under natural conditions. if similar conditions can be induced in uninfected individuals, it will contribute the first line of protection against hiv-1 infection, and also improve the effects of anti-hiv-1 vaccines. we reasoned that in ... | 2010 | 20719993 |
| biofilm formation and composition on different implant materials in vivo. | biofilm formation was evaluated on the following titanium and zirconia implants in vivo: machined titanium (ti-m), modified titanium (tiunite), modified zirconia (ziunite), machined alumina-toughened zirconia (atz-m), sandblasted alumina-toughened zirconia (atz-s), and machined zirconia (tzp-a-m). bovine enamel slabs were used as controls. surface morphologies were examined by atomic force (afm) and scanning electron microscopy (sem). the surface wettability was also determined. twelve healthy v ... | 2010 | 20725954 |
| subgingival microbial profiles of smokers with periodontitis. | the subgingival microbiome is largely uncultivated, and therefore, cultivation-based and targeted molecular approaches have limited value in examining the effect of smoking on this community. we tested the hypothesis that the subgingival biofilm is compositionally different in current and never-smokers by using an open-ended molecular approach for bacterial identification. subgingival plaque from deep sites of current and never-smokers matched for disease was analyzed by 16s sequencing. smokers ... | 2010 | 20739702 |
| symbiotic bacteria induced necrotizing pancreatitis. | intestinal flora and anaerobes are frequently implicated in causing infectious necrotizing pancreatitis however bifidobacterium and veillonella have rarely been isolated as the causative agents. bifidobacterium and veillonella are commensal anaerobes which reside in gastrointestinal tract and help deconjugate bile acids. bifidobacterium is also frequently used in probiotics. | 2010 | 20818120 |
| the bacterial microbiota in the oral mucosa of rural amerindians. | the oral microbiota plays an important role in buccal health and in diseases such as periodontitis and meningitis. the study of the human oral bacteria has so far focused on subjects from western societies, while little is known about subjects from isolated communities. this work determined the composition of the oral mucosa microbiota from six amazon amerindians, and tested a sample preservation alternative to freezing. paired oral swabs were taken from six adults of guahibo ethnicity living in ... | 2010 | 20847007 |
| association of symptoms with gastrointestinal microbiota in irritable bowel syndrome. | to investigate the correlations between self-reported symptoms of irritable bowel syndrome (ibs) and the gastrointestinal (gi) microbiota composition. | 2010 | 20857523 |
| clonal analysis of the microbiota of severe early childhood caries. | severe early childhood caries is a microbial infection that severely compromises the dentition of young children. the aim of this study was to characterize the microbiota of severe early childhood caries. | 2010 | 20861633 |
| brewer's yeast cell wall affects microbiota composition and decreases bacteroides fragilis populations in an anaerobic gut intestinal model. | brewer's yeast cell wall (byc) has been reported to have prebiotic activity that improves the microbiotal composition of the human gut. to understand the precise effect of byc on gut microbiota and its metabolism, we used a three-stage continuous-flow reactor system that mimicked the environment of the large intestine. the reactor system was able to maintain the bacterial community stably for a week. the bacteroides fragilis population decreased drastically after the addition of byc into this sy ... | 2010 | 20926339 |
| human oral, gut, and plaque microbiota in patients with atherosclerosis. | periodontal disease has been associated with atherosclerosis, suggesting that bacteria from the oral cavity may contribute to the development of atherosclerosis and cardiovascular disease. furthermore, the gut microbiota may affect obesity, which is associated with atherosclerosis. using qpcr, we show that bacterial dna was present in the atherosclerotic plaque and that the amount of dna correlated with the amount of leukocytes in the atherosclerotic plaque. to investigate the microbial composit ... | 2010 | 20937873 |
| effect of biofilm on the repair bond strengths of composites. | composite restorations degrade during wear, but it is unknown how wear affects the composite surface and influences composite-to-composite bonding in minimally invasive repair. here, it is hypothesized that in vitro exposure of composites to oral biofilm yields clinically relevant degradation of composite surfaces, and its influence on composite-to-composite bonding is determined. biofilms on composite surfaces in vitro increased their roughness and decreased filler particle exposure, except for ... | 2010 | 20940354 |
| starch-entrapped microspheres show a beneficial fermentation profile and decrease in potentially harmful bacteria during in vitro fermentation in faecal microbiota obtained from patients with inflammatory bowel disease. | the purpose of this research was to test the hypothesis that starch-entrapped microspheres would produce favourable fermentation profiles and microbial shifts during in vitro fermentation with the faecal microbiota from patients with inflammatory bowel disease (ibd). in vitro fermentation was carried out using a validated, dynamic, computer-controlled model of the human colon (toegepast natuurwetenschappelijk onderzoek gastro-intestinal model-2) after inoculation with pooled faeces from healthy ... | 2010 | 20021704 |
| in vitro killing activity of crevicular concentrations of tinidazole plus common oral antibiotics against high-density mixed inocula of periodontal pathogens in strict anaerobic conditions. | odontogenic infections are polymicrobial. this study explores the in vitro killing activity by concentrations similar to those found in crevicular fluid of tinidazole in combination with amoxicillin/clavulanic acid, clindamycin and levofloxacin against four groups of high-density mixed inocula of anaerobes (prevotella buccae, fusobacterium nucleatum, and veillonella spp.) and facultative (capnocytophaga spp. and streptococcus spp.) isolates of periodontal pathogens. | 2010 | 20059425 |
| complete genome sequence of veillonella parvula type strain (te3). | veillonella parvula (veillon and zuber 1898) prévot 1933 is the type species of the genus veillonella in the family veillonellaceae within the order clostridiales. the species v. parvula is of interest because it is frequently isolated from dental plaque in the human oral cavity and can cause opportunistic infections. the species is strictly anaerobic and grows as small cocci which usually occur in pairs. veillonellae are characterized by their unusual metabolism which is centered on the activit ... | 2010 | 21304678 |
| altered profiles of intestinal microbiota and organic acids may be the origin of symptoms in irritable bowel syndrome. | the profile of intestinal organic acids in irritable bowel syndrome (ibs) and its correlation with gastrointestinal (gi) symptoms are not clear. we hypothesized in this study that altered gi microbiota contribute to ibs symptoms through increased levels of organic acids. | 2010 | 19903265 |
| partitioning core and satellite taxa from within cystic fibrosis lung bacterial communities. | cystic fibrosis (cf) patients suffer from chronic bacterial lung infections that lead to death in the majority of cases. the need to maintain lung function in these patients means that characterising these infections is vital. increasingly, culture-independent analyses are expanding the number of bacterial species associated with cf respiratory samples; however, the potential significance of these species is not known. here, we applied ecological statistical tools to such culture-independent dat ... | 2010 | 21151003 |
| microbiota of dentinal caries as assessed by reverse-capture checkerboard analysis. | this study aimed to identify the microbiota of different layers of dentinal caries by using a culture-independent molecular biology approach. | 2010 | 21160186 |
| microbiological profiles in saliva and supragingival plaque from caries-active adolescents before and after a short-term daily intake of milk supplemented with probiotic bacteria - a pilot study. | the aim of the present pilot study was to investigate the microbial profile in saliva and supragingival plaque samples collected from caries-active adolescents before and after a daily short-term intake of milk supplemented with the probiotic bacteria. | 2010 | 21180676 |
| comparative evaluation of bactericidal potential of four root canal filling materials against microflora of infected non-vital primary teeth. | since complete debridement of the root canals of the primary teeth is not practically possible due to the highly variable root canal anatomy, success of the endodontic therapy depends partly on the use ofantibacterial irrigating agents and root canal filling materials. recent literature indicates that anaerobes comprise a majority of the bacteria in necrotic root canals ofprimary teeth. the study determined the antibacterial effectiveness of four root canal filling materials namely calcium hydro ... | 2010 | 21189760 |
| change in diet and oral hygiene over an 8-week period: effects on oral health and oral biofilm. | the aim of the study was to monitor changes in oral health and oral biofilm composition in vivo during an experiment simulating prehistoric lifestyle and diet and poor oral hygiene. thirteen subjects lived for a period of 8 weeks under neolithic conditions. the following clinical parameters were recorded before and after the project: gingival and plaque index (löe and silness, acta odontol scand 21:533, 1963; silness and löe, acta odontol scand 22:121-135, 1964), probing pocket depth, and bleedi ... | 2010 | 19626350 |
| bacteriological aspects implicated in abdominal surgical emergencies. | the purpose of the present study was to establish the microbial etiology of abdominal surgical emergencies as well as the relationship between the bacterial etiology and the virulence factors produced by the respective isolated strains. 110 bacterial strains were isolated from 100 randomized clinical cases, operated during 2009-2010 in the first surgical clinic of the university hospital of bucharest. the clinical cases (sex ratio 52 m/48f aged between 22-85 years old) were classified into three ... | 2010 | 21351699 |
| the role of bacteria in oral cancer. | despite the widening interest in the possible association between bacteria and different stages of cancer development, our knowledge in its relation to oral cancers remains inadequate. the aim of this review article is to derive a better understanding on the role of various micro-organisms in the etiogenesis of oral cancers through all the available data on the pubmed. different bacteria have been proposed to induce carcinogenesis either through induction of chronic inflammation or by interferen ... | 2010 | 21584217 |
| detection of nine microorganisms from the initial carious root lesions using a taqman-based real-time pcr. | oral diseases (2011) objective: the purpose of this study was to quantify nine selected cariogenic bacteria in plaque from sound root surfaces and initial carious root lesions using taqman pcr and to analyse a putative dependence on the kind of initial periodontal treatment. material and methods: fifty-four subjects with generalized chronic periodontitis were randomly allocated to one of the following initial periodontal therapies: full-mouth disinfection, full-mouth scaling and root planing o ... | 2011 | 21605286 |
| veillonella spondylodiscitis in a healthy 76-year-old lady. | purpose: to report a case of veillonella spondylodiscitis in a healthy 76-year-old lady. methods: a previously healthy 76-year-old lady presented with worsening axial back pain at the thoracolumbar junction, fever and loss of weight. examination revealed deep tenderness over the thoracolumbar junction with painful and restricted spinal movements. the lower limb motor power, sensation and reflexes were normal. results: radiographs of the lumbosacral spine showed evidence of spinal instability wit ... | 2011 | 21674211 |
| anaerobic bacteria colonizing the lower airways in lung cancer patients. | anaerobes comprise most of the endogenous oropharyngeal microflora, and can cause infections of airways in lung cancer patients who are at high risk for respiratory tract infections. the aim of this study was to determine the frequency and species diversity of anaerobes in specimens from the lower airways of lung cancer patients. sensitivity of the isolates to conventional antimicrobial agents used in anaerobe therapy was assessed. respiratory secretions obtained by bronchoscopy from 30 lung can ... | 2011 | 21744326 |
| pyrosequencing-based molecular monitoring of the intestinal bacterial colonization in preterm infants. | objectives:: to investigate the previously unexplored diversity of neonatal intestinal microbiota and monitor early intestinal colonization patterns in korean preterm infants using high-throughput pyrosequencing technology combined with 16s rdna- based molecular methods methods:: a total of 46,369 partial 16s rdna sequences obtained from 30 fecal samples serially taken from 10 very low birth weight preterm infants were analyzed. results:: a significant proportion of the molecular species (21.9%) ... | 2011 | 21734604 |
| oral microbiome profiles: 16s rrna pyrosequencing and microarray assay comparison. | the human oral microbiome is potentially related to diverse health conditions and high-throughput technology provides the possibility of surveying microbial community structure at high resolution. we compared two oral microbiome survey methods: broad-based microbiome identification by 16s rrna gene sequencing and targeted characterization of microbes by custom dna microarray. | 2011 | 21829515 |
| genetic transformation of veillonella parvula. | veillonellae are one of the most prevalent and predominant microorganisms in both the supra- and subgingival plaques of the human oral cavity. veillonellae's mutualistic relationships with the early, middle, and late colonizers of the oral cavity make them an important component of oral biofilm ecology. unlike other ubiquitous early colonizers in the oral cavity, surprisingly little is known about veillonella biology due to our lack of ability to genetically transform this group of bacteria. the ... | 2011 | 21707732 |
| enteral tube feeding alters the oral indigenous microbiota in elderly adults. | enteral tube feeding is widely used to maintain nutrition for elderly adults with eating difficulties, but its long-term use alters the environment of the oral ecosystem. this study characterized the tongue microbiota of tube-fed elderly adults by analyzing the 16s rrna gene. the terminal restriction fragment length polymorphism (t-rflp) profiles of 44 tube-fed subjects were compared with those of 54 subjects fed orally (average age 86.4 -¦ 6.9 years). barcoded pyrosequencing data were also obta ... | 2011 | 21821752 |
| phenotypic identification of over 1000 isolates of anaerobic bacteria recovered between 1999 and 2008 in a major costa rican hospital. | because of limitations in infrastructure, the aetiology of infections caused by anaerobic bacteria is seldom determined in clinical laboratories of developing countries. this study reports on the identification of 1010 anaerobic bacterial isolates collected between 1999 and 2008 in a major costa rican hospital with the use of two commercial phenotypic systems (rapid 32a and api 20a). approximately 60% of the isolates were gram-positive and, among the 35 species of gram-positive bacteria found, t ... | 2011 | 21722256 |