Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| mechanisms of apoptosis in murine fibroblasts by two intracellular protozoan parasites, toxoplasma gondii and neospora caninum. | studies to clarify the mechanisms of apoptosis in host cells, a31 (balb/3t3 clone a31 fibroblasts), caused by two intracellular protozoan parasites, toxoplasma gondii and neospora caninum, were carried out in an in vitro system. the viability of n. caninum-infected cells was significantly reduced following treatment with mouse interferon (ifn)-gamma. by contrast, mouse ifn-gamma treatment had no significant effect on the induction of apoptosis in t. gondii-infected cells. apoptosis of n. caninum ... | 2002 | 12164820 |
| characterization of an outbred pregnant mouse model of neospora caninum infection. | fetal loss and vertical transmission of neospora caninum were evaluated in outbred quackenbush (qs) mice with respect to dose of parasites, n. caninum isolate, and route of injection. mice were infected with nc-liverpool or nc-sweb1 at day 5 or 8 of pregnancy with doses of 10(4), 10(6), or 10(7) parasites, through either a subcutaneous or intraperitoneal injection. polymerase chain reaction was used to detect n. caninum in the brains of offspring, and enzyme-linked immunosorbent assay was used t ... | 2002 | 12197115 |
| cutaneous neosporosis during treatment of pemphigus foliaceus in a dog. | a 4-year-old, intact male rottweiler was presented with a 10-day history of papulonodular dermatitis. at the time of presentation, the dog was receiving prednisone and azathioprine to treat pemphigus foliaceus. cutaneous neosporosis was diagnosed by immunohistochemistry on skin biopsy specimens and a high serum antibody titer to neospora caninum by neospora agglutination test. electron microscopy examination of skin specimens further supported the diagnosis. clindamycin therapy, together with wi ... | 2002 | 12220024 |
| occurrence of neospora caninum antibodies in sera from dogs of the city of são paulo, brazil. | neospora caninum is an important cause of abortion in dairy cattle worldwide. dogs are important in the epidemiology of this parasite because they are the only hosts known to excrete n. caninum oocysts. in order to understand the prevalence of n. caninum in dogs, sera from 500 owned dogs and from over 600 feral street dogs from the city of são paulo, brazil were assayed for antibodies to n. caninum. sera were examined by the neospora agglutination test (nat) using mouse-derived tachyzoites. anti ... | 2002 | 12031819 |
| direct production losses and treatment costs from bovine viral diarrhoea virus, bovine leukosis virus, mycobacterium avium subspecies paratuberculosis, and neospora caninum. | our purpose was to determine direct production losses (milk loss, premature voluntary culling and reduced slaughter value, mortaliy loss, and abortion and reproductive loss) and treatmetn costs (veterinary services, medication cost, and extra farm labour cost) due to four infectious diseases in the maritime provinces of canada: bovine viral diarrhoea (bvd), enzootic bovine leukosis (ebl), johne's disease (jd), and neosporosis. we used a partial-budget model, and incorporated risk and sensitivity ... | 2002 | 12350317 |
| steroid hormones do not reactivate neospora caninum in ovariectomized mice. | the direct effects of three steroid hormones (progesterone, estradiol-17beta and corticosterone) on the growth of neospora caninum (n. caninum) tachyzoite were examined in vero cells. subsequently, ovariectomized balb/c mice infected with n. caninum were treated with physiological concentrations of the steroid hormones for 1 or 2 weeks. these hormones had no direct effect on the parasite growth in vitro. in the infected mice, there was no significant difference in the parasite distribution and h ... | 2002 | 12399600 |
| prevalence of antibodies to neospora caninum in korean native beef cattle. | a total of 438 sera from korean native beef cattle in 9 provinces were tested for neospora caninum antibodies using an immunofluorescent antibody test (ifat). eighteen (4.1%) cattle were positive by ifat. the titers ranged from 1:200 (10 animals), 1:400 (5 animals), 1:800 (2 animals) to 1:1,600 (1 animal). although the seroprevalence was slightly higher in chungnam (8.9%), this was not significantly different from those noted in kyunggi, kangwon, kyungbuk, kyungnam, and cheju provinces. sera obt ... | 2002 | 12419873 |
| management factors related to seroprevalences to bovine viral-diarrhoea virus, bovine-leukosis virus, mycobacterium avium subspecies paratuberculosis, and neospora caninum in dairy herds in the canadian maritimes. | bovine viral-diarrhoea (bvd), enzootic bovine leukosis (ebl), johne's disease (jd), and neosporosis lower on-farm productivity, reduce export competitiveness, and increase consumer concerns regarding safety. our purpose was to examine the relationship between 27 control practices and the estimated true seroprevalences for these four diseases for 2604 cattle in 90 dairy herds in the maritimes provinces of canada. overall, 37.8, 20.4, 3.4, and 19.2% of all sampled cattle were truly exposed to the ... | 2002 | 12324207 |
| susceptibility of psammomys obesus and meriones tristrami to tachyzoites of neospora caninum. | the susceptibility of psammomys obesus (sand rat) and meriones tristrami (tristram's jird) to neospora caninum was investigated by subcutaneous (s.c.) and intraperitoneal (i.p.) inoculation of 10-fold doses of culture-derived tachyzoites. groups of 5 animals were inoculated with doses of 10-10(7) parasites via each route of inoculation. all but 2 of the sand rats inoculated with doses of 10-10(4) parasites succumbed to the infection by 7-18 days postinfection. all jirds inoculated with 10(7) tac ... | 2002 | 12054004 |
| neospora caninum: a cause of immune-mediated failure of pregnancy? | resistance to many intracellular protozoan parasites is dependent on t helper cell 1 cytokine responses. this has important repercussions for pregnant females because strong t helper cell 1 cytokine responses are incompatible with successful pregnancy. thus, there are two possible consequences of infection with protozoans such as leishmania major, plasmodium falciparum and toxoplasma gondii during pregnancy: (1) pregnancy is compromised; or (2) resistance to the parasite is compromised. the apic ... | 2002 | 12377255 |
| neospora caninum: oocyst challenge of pregnant cows. | three pregnant cows were each orally challenged at 10 weeks of gestation with 600 sporulated oocysts of neospora caninum. the number of oocysts was limited by those available. in concurrent bioassays, one oocyst per os infected each of two gerbils. challenged cattle developed neospora-specific antibody, cell proliferation and gamma-interferon responses. n. caninum specific pcr demonstrated persisting infection in the brains of cows 4 months after calving. abortion was not induced and there was n ... | 2002 | 12383634 |
| neospora caninum serostatus and culling of holstein cattle. | to determine whether time until culling or risk of culling was associated with neospora caninum serostatus among holstein cattle in dairy herds in ontario. | 2002 | 12387388 |
| red foxes (vulpes vulpes) are a natural intermediate host of neospora caninum. | the present study was undertaken to determine if red foxes are natural intermediate and/or definitive host for neospora caninum and to study the importance of infection of n. caninum in this species in north-eastern spain. faecal samples and brain tissues were obtained from 122 foxes from 21 rural areas of catalonia. faeces collected were examined for parasite eggs and coccidian oocysts using sucrose flotation. for pcr-based diagnosis of n. caninum in brain tissues, the specific genomic nc5 regi ... | 2002 | 12163240 |
| confirmation of the prevention of vertical transmission of neospora caninum in cattle by the use of embryo transfer. | 2002 | 12224621 | |
| risk of abortion associated with neospora caninum during different lactations and evidence of congenital transmission in dairy cows. | to examine the relationship between exposure to neospora caninum and abortion in dairy cows during their first, second, third, and fourth or later lactations and to establish the main mode of transmission in female calves from birth until their first pregnancy was terminated by abortion or parturition. | 2002 | 12494973 |
| the influence of feeding and maintenance system on occurrence of toxoplasma gondii infections in dogs. | serum samples of 113 dogs visiting "outpatient clinics", 52 dogs kept in shelters and 35 animals from a military dog training centre were examined for toxoplasma gondii specific antibodies using a latex agglutination test. significant differences in seroprevalences were found between dogs from the training centre (8.6% of positive results) and the other populations examined (40.7% of positive seroreagents in animals visiting outpatient clinics and 44.2% in the group from shelters, respectively). ... | 2002 | 12512556 |
| neospora caninum ncsrs2 is a transmembrane protein that contains a glycosylphosphatidylinositol anchor in insect cells. | we investigated the terminal location of ncsrs2, a surface antigen of neospora caninum that has potential use for diagnosis, and demonstrated its importance as a vaccine component against neosporosis, in an insect-baculovirus expression system. to examine the role of the hydrophobic c-terminal tail in ncsrs2, four types of recombinant baculoviruses were constructed. immunoblotting and n-terminal amino acid analysis revealed cleavage of a 6 kda of the n-terminal signal peptide in the mature ncsrs ... | 2002 | 12423932 |
| neospora caninum serostatus and milk production of holstein cattle. | to determine whether neospora caninum serostatus was associated with milk production among holstein cattle in ontario. | 2002 | 12387387 |
| quantitative detection of neospora caninum in bovine aborted fetuses and experimentally infected mice by real-time pcr. | we report the development of a real-time pcr assay for the quantitative detection of neospora caninum in infected host tissues. the assay uses the double-stranded dna-binding dye sybr green i to continuously monitor product formation. oligonucleotide primers were designed to amplify a 76-bp dna fragment corresponding to the nc5 sequence of n. caninum. a similar method was developed to quantify the 28s rrna host gene in order to compare the parasite load of different samples and to correct for th ... | 2002 | 11923330 |
| in contrast to dogs, red foxes (vulpes vulpes) did not shed neospora caninum upon feeding of intermediate host tissues. | to clarify whether red foxes (vulpes vulpes) can be final hosts of neospora caninum, foxes and dogs were fed in parallel on tissues of a sheep and a goat experimentally infected with n. caninum. the faeces of at least two of five dogs contained n. caninum oocysts, as determined by bioassay. in the faeces of all six foxes fed in parallel, oocysts were detected that were larger in size (length 12.6 +/- 0.5 microm, width 11.8 +/- 0.4 microm) than the oocysts shed by the dogs. ribosomal rna sequence ... | 2002 | 11822736 |
| neospora caninum and hammondia heydorni are separate species/organisms. | neospora caninum and hammondia heydorni are two coccidian parasites with morphologically similar oocysts in canine feces. it was recently proposed that they are one species. in this paper, we review the biology and morphology of these parasites and present evidence that n. caninum and h. heydorni are separate species. | 2002 | 11832296 |
| neospora 2001. | infection with the protozoan parasite neospora caninum is emerging as a major cause of reproductive loss in cattle worldwide. recent advances in research with the ultimate goal of devising effective and sustainable control measures were discussed at neospora 2001. neospora 2001 was held, 13-15 september 2001, at the moredun research institute, edinburgh, uk. the international meeting covered many aspects of bovine neosporosis including diagnosis, epidemiology and immunology. | 2002 | 11850000 |
| neospora caninum and waddlia chondrophila strain 2032/99 in a septic stillborn calf. | characteristics of an intracellularly growing micro-organism isolated from an aborted bovine foetus are described. the organism replicated within cytoplasmic vacuoles, was resistant to penicillin and exhibited structural characteristics compatible with waddlia chondrophila. an elisa specific for chlamydia spp., immunofluorescence tests using antibodies directed against chlamydia spp. or simkania negevensis, and pcr using chlamydia-specific primers showed that the agent was distinct from chlamydi ... | 2002 | 11852195 |
| vaccine development against neospora caninum infection. | neospora caninum is a recognized protozoan parasite of a wide range of mammalian hosts, and was reported for the first time in 1988. the isolation of its oocysts in dog's faeces in 1998 led to its establishment as a parasitic species undergoing typical coccidian life cycle. infection with n. caninum causes paralysis and death in young livestock and companion animals, and is associated with abortions and stillbirth in cattle, and neurologic disease in calves. considering the economic and agricult ... | 2002 | 11853138 |
| neosporosis with cerebellar involvement in an adult dog. | a case of an adult dog with multifocal, progressive neurological signs caused by neospora caninum is reported. pathological studies showed cerebellar lesions due to the parasite, which was also present in other parts of the nervous system and muscle. cerebellar atrophy related to neospora infection has been rarely reported in veterinary medicine, and has been shown to affect ruminants and dogs. the cerebellar involvement and the age of the present dog make this case uncommon. | 2002 | 11873953 |
| point source exposure of cattle to neospora caninum consistent with periods of common housing and feeding and related to the introduction of a dog. | eight dairy herds with evidence of post-natal transmission of neospora caninum were used to test the hypothesis of a point source exposure by a retrospective analysis of the housing and feeding of infected age-groups. the first n. caninum-associated abortion or birth of n. caninum-seropositive offspring from the post-natally infected age-group was considered as the first indication of the infection. in seven of the eight dairy herds, a point source exposure to n. caninum of the infected age-grou ... | 2002 | 11900922 |
| natural transmission routes of neospora caninum between farm dogs and cattle. | twelve dairy herds with evidence of post-natal infection with neospora caninum were compared with 21 control herds with no evidence of post-natal infection. on the former farms, dogs consumed placenta or licked uterine discharge in 75 and 67% of the farms, respectively, while on control farms these activities occurred in 38 and 24% of the farms, respectively. on all control farms and all but three post-natally infected farms the dogs were fed colostrum or milk. defecation of dogs on the feeding ... | 2002 | 11900923 |
| pattern of recognition of neospora caninum tachyzoite antigens by naturally infected pregnant cattle and aborted foetuses. | different aspects of neospora tachyzoite antigen recognition by neospora-infected heifers and cows and aborted foetuses were studied. the pattern of antigen recognition and the relationship between ifat titres and number of neospora antigens detected, were evaluated. in addition, the tachyzoite antigens involved in the humoral immune response developed against infection in normal cows and cows that aborted were also characterised throughout pregnancy. comparison of tachyzoite antigen recognition ... | 2002 | 12072210 |
| a method of probability diagnostic assignment that applies bayes theorem for use in serologic diagnostics, using an example of neospora caninum infection in cattle. | to develop a method of probability diagnostic assignment (pda) that uses continuous serologic measures and infection prevalence to estimate the probability of an animal being infected, using neospora caninum as an example. | 2002 | 11911564 |
| studies on serological cross-reaction of neospora caninum with toxoplasma gondii and hammondia heydorni. | the enzyme-linked immunosorbent assay (elisa) was used to examine cross-reactivity of neospora caninum with toxoplasma gondii and hammondia heydorni. anti-t. gondii mouse and cat sera cross-reacted with n. caninum soluble antigen (nla), but not with the recombinant surface antigen (ncsrs2). anti-h. heydorni dog sera showed no cross-reactivity with either the nla antigen or the ncsrs2. lack of cross-reactivity between anti-h. heydorni sera and n. caninum antigens, and the cross-reactivity of anti ... | 2002 | 11913555 |
| effect of neospora caninum on in vitro development of preimplantation stage bovine embryos and adherence to the zona pellucida. | 2002 | 11913589 | |
| necrotizing cerebellitis due to neospora caninum infection in an old dog. | a severe, necrotizing, non-suppurative inflammation of the cerebellum associated with neospora caninum infection was identified in a 14-year-old male labrador retriever. on presentation, clinical signs included mild depression and head tremor, marked ataxia of both thoracic and pelvic limbs, and abnormal postural reactions. in the central nervous system, inflammatory lesions were mainly restricted to the cerebellar leptomeninges and cerebellar cortex, which appeared necrotic and atrophic. protoz ... | 2002 | 11913826 |
| improved production of neospora caninum oocysts, cyclical oral transmission between dogs and cattle, and in vitro isolation from oocysts. | scarce information is available about neospora caninum oocysts and sporozoites, in part because only small numbers of oocysts have typically been produced by experimentally infected dogs. we hypothesized that i reason for low experimental production of oocysts is that dogs have been fed tissues from experimentally infected mice instead of tissues from cattle (which are natural intermediate hosts of n. caninum). in this study, 9 dogs were fed tissues from n. caninum-infected calves, and oocyst pr ... | 2002 | 12537111 |
| prevalence of antibodies to neospora caninum and sarcocystis neurona in sera of domestic cats from brazil. | parasite biology, epidemiology and systematics laboratory, animal and natural resources institute, agricultural research service, united states department of agriculture, building 1001, beltsville, maryland 20705-2350 antibodies to neospora caninum and sarcocystis neurona were determined in serum samples of 502 domestic cats from brazil using direct agglutination tests with the respective antigens. antibodies to s. neurona were not found in 1:50 dilution of any serum in the s. neurona agglutinat ... | 2002 | 12537122 |
| control options for neospora caninum infections in cattle--current state of knowledge. | this article reviews control options for neospora caninum infection and abortion in cattle, drawing on published literature and the authors' own research in this field. apart from the successful use of embryo transfer to prevent congenital infection in calves born to infected cows, there are currently no accepted control methods for the prevention of abortions in cattle. the epidemiological data at hand suggest that concomitant infections with bovine pestivirus increase the risk of abortion sign ... | 2002 | 16032217 |
| recent advances in understanding the epidemiology of neospora caninum in cattle. | introduction: neospora caninum is the most frequently diagnosed cause of cattle abortion in new zealand and is an important pathogen worldwide. the parasite has been found in aborted bovine foetuses and in puppies with neurological disease. recent developments have provided new insights into the epidemiology of the disease that are reviewed in this article. transmission: transplacental infection is of major importance in the spread of n. caninum overseas. most congenital infections result in the ... | 2001 | 16032161 |
| first confirmed case of neospora caninum-associated abortion outbreak in portugal. | in a dairy herd experiencing an abortion outbreak, 49% of the 119 cows were positive for neospora caninum antibodies and the remaining 51% were seronegative. using immunohistochemical analysis of foetal brain tissue, neospora cysts and bradyzoite clusters were identified in two of the 15 aborted foetuses submitted for testing. other agents that commonly cause abortions were not found. these results, coupled with neurological clinical symptoms in new-born calves, implicated n. caninum as the majo ... | 2001 | 11928926 |
| serodiagnosis of toxoplasma gondii infection in cats by enzyme-linked immunosorbent assay using recombinant sag1. | the gene encoding surface antigen 1 (sag1, p30) of toxoplasma gondii (t. gondii) was cloned into the plasmid pgex-4t-3 and subsequently expressed in escherichia coli (e. coli) as a glutathione-s-transferase (gst) fusion protein. the recombinant sag1 (rsag1) was refolded using 8m urea solution followed by dialysis and thereafter evaluated in an enzyme-linked immunosorbent assay (elisa) for serological diagnosis of toxoplasmosis. the test sera were adsorbed with gst to block non-specific reactivit ... | 2001 | 11705650 |
| prevalence of agglutinating antibodies to neospora caninum in raccoons, procyon lotor. | neospora caninum is an apicomplexan parasite that causes neonatal neuromuscular disease in dogs and abortions in cattle. dogs are the only proven definitive host. little is known about the prevalence of antibodies to this parasite in wildlife. sera from 99 raccoons (procyon lotor) were examined for agglutinating antibodies to n. caninum using the modified agglutination test employing formalin-fixed tachyzoites as antigen. raccoons originated in florida (n = 24, collected in 1996), new jersey (n ... | 2001 | 11695399 |
| cyclic transmission of neospora caninum: serological findings in dogs shedding oocysts. | in a previous paper we demonstrated that hammondia heydorni-like oocysts isolated in 1996 from a naturally infected dog could not be distinguished from the isolate neospora caninum nc-1. the isolate, designated as h. heydorni-berlin-1996, was cyclically transmitted using dogs as the final hosts. the present study provides information on the antibody responses of the dogs used for the cyclical transmission of this isolate. the majority of dogs that had shed oocysts showed no sero-conversion with ... | 2001 | 11688895 |
| comparative molecular investigation of nc5-pcr amplicons from neospora caninum nc-1 and hammondia heydorni-berlin-1996. | the clinical relevance of neospora caninum as a cyst-forming coccidian parasite is increasingly acknowledged within veterinary medicine, although the pathways of transmission are far from being solved. the parasite is well known for causing diaplacental infections in cows associated with abortion and/or severe damage of the fetus. in addition, it may cause neuromuscular disease in dogs, which thus apparently act as intermediate hosts as well as final hosts. in our previous studies, we have demon ... | 2001 | 11688897 |
| a swiss case-control study to assess neospora caninum-associated bovine abortions by pcr, histopathology and serology. | neospora caninum is one of the most frequent infectious organisms causing abortion in cattle worldwide. the present case-control study was designed to assess the importance of bovine neosporosis for causing abortion in swiss cattle and to identify selected risk factors. infection was primarily diagnosed by a n. caninum-specific pcr and serology, complemented with histopathology and immunohistochemistry. a total of 113 case and 113 corresponding control-farms were studied for 1.5 year. during thi ... | 2001 | 11705647 |
| neospora caninum in persistently infected, pregnant cows: spontaneous transplacental infection is associated with an acute increase in maternal antibody. | nine cows which were naturally and persistently infected with neospora caninum were housed and observed intensively throughout pregnancy. no recrudescence of a latent infection was detected by pcr tests on maternal blood but fetal infection, implying a recrudescence of maternal parasitosis, was associated with a marked increase in maternal antibody. the increase occurred in the second half of pregnancy in five cows which infected their calves, and before mid-pregnancy in one cow which aborted. t ... | 2001 | 11688746 |
| granulomatous encephalitis in a neurologically impaired goat kid associated with degeneration of neospora caninum tissue cysts. | congenital neospora caninum infection was diagnosed in a saanen goat from a farm in southern brazil. the kid was unable to nurse and had difficulty rising, ataxia, and opistothotonos. the neurologic signs became more severe 3 days after birth, when it was euthanized. no gross lesions were observed at necropsy. multifocal infiltrates primarily of mononuclear cells, nodular microgliosis, and perivascular cuffs of lymphocytes, plasma cells, and few neutrophils were observed in the brain, mostly in ... | 2001 | 11580064 |
| hammondia heydorni-like oocysts shed by a naturally infected dog and neospora caninum nc-1 cannot be distinguished. | this study describes transmission experiments using hammondia heydorni-like oocysts isolated in 1996 from a naturally infected dog. the isolate was designated as h. heydorni-berlin-1996. examination of sera from infected intermediate hosts showed immunoblot reactions that resembled patterns observed after neospora caninum nc-1 infection. furthermore, n. caninum dna could be demonstrated in tissue samples (e.g. heart, brain) of experimentally infected intermediate hosts and in oocyst preparations ... | 2001 | 11688886 |
| association of serologic status for neospora caninum and postweaning feed efficiency in beef steers. | to determine the effects of serologic status for neospora caninum on short-term weight gain, feed intake, and feed efficiency (feed intake/gain). | 2001 | 11697371 |
| immune responses in pregnant cattle and bovine fetuses following experimental infection with neospora caninum. | humoral and cell-mediated immune (cmi) responses [i.e. proliferative responses and gamma interferon (ifn-gamma) production], were elicited in five cows infected between 159 and 169 days of gestation by a combined intravenous-intramuscular inoculation of neospora caninum tachyzoites. analysis of antigen-specific immunoglobulin (igg) subclasses revealed a predominant igg2 response in two cows, a mixed igg1-igg2 response in two other cows and a predominant igg1 response in one cow. no correlation w ... | 2001 | 11688887 |
| prevention of vertical transmission of neospora caninum in balb/c mice by recombinant vaccinia virus carrying ncsrs2 gene. | neospora caninum infection is the major cause of bovine abortion. to develop a vaccine against n. caninum infection, recombinant vaccinia viruses carrying ncsrs2 and ncsag1 genes (vv/nc-p43 and vv/nc-p36, respectively) were constructed and were tested in a mouse model. vaccination of dams with vv/nc-p43 appeared to confer effective protection against vertical transmission to offspring, though that with vv/nc-p36 only provided partial protection. moreover, the vv/nc-p43 vaccination provoked cellu ... | 2001 | 11166895 |
| isolation of neospora caninum genes detected during a chronic murine infection. | in order to isolate genes coding for antigens of neospora caninum which are recognised by the host immune system during a chronic murine infection, a cdna library was immunoscreened with pooled sera from mice which survived three independent infections by n. caninum. two new genes from n. caninum were isolated and expressed in escherichia coli. the genes identified include one homologous to gra1 of toxoplasma gondii, plus another (ncp20) previously unknown in any taxon. both genes encode small p ... | 2001 | 11165273 |
| protective efficacy of vaccination by recombinant vaccinia virus against neospora caninum infection. | the recombinant vaccinia viruses expressing the surface protein of neospora caninum tachyzoite, ncsag1 or ncsrs2, were constructed. the vaccination with these recombinant viruses could protect effectively the parasite invasion in a mouse model system. the vaccine efficacy of ncsrs2 was higher than that of ncsag1. the present study indicated that a high level of igg1 ab production to parasite is important for clearance of parasite at the early stage of infection and that t cell response has a cru ... | 2001 | 11163660 |
| do cows protect fetuses from neospora caninum transmission? | 2001 | 11137733 | |
| neosporosis in mexican dairy herds: lesions and immunohistochemical detection of neospora caninum in fetuses. | of 211 aborted bovine fetuses collected from mexican dairy herds between january 1996 and march 1999, 73 showed microscopical lesions consistent with neosporosis. of these 73 fetuses, 58 (79%) showed lymphocytic myocarditis, 39 (53%) showed microgliosis and multifocal necrosis in the brain, 39 (53%) showed lymphocytic hepatitis, and 19 (26%) showed lymphocytic myositis. immunohistochemical examination of brain, myocardium and liver from 53 of the same 73 fetuses demonstrated neospora caninum ant ... | 2001 | 11437517 |
| postnatal neosporosis in dairy cattle in northeast thailand. | a total of 83 dairy cows in loei province (muang) and nong bua lamphu (nbl) province, northeast thailand were sampled three times within 6 months in 1998 and their sera were examined for antibodies to neospora caninum at a dilution of 1:100 in the indirect fluorescent antibody test (ifat). in muang the seroprevalence of n. caninum was 37.5% (june), 60% (august), and 62.5% (november). in nbl the prevalence was 50% (august) and 70% (november). in both areas abortions were observed between 1 and 3 ... | 2001 | 11113552 |
| a previous infection with toxoplasma gondii does not protect against a challenge with neospora caninum in pregnant sheep. | sheep immunized with toxoplasma gondii (toxovax) prior to pregnancy were tested for their ability to withstand a challenge at 90 days gestation with 107 neospora caninum (nc1) tachyzoites. the antibody responses in sheep following immunization with t. gondii were specific for t. gondii whereas peripheral blood mononuclear cells responded to both t. gondii and n. caninum antigen in vitro. this suggested that there was induction of crossreactive immune recognition in the sheep, at least at the cel ... | 2001 | 11240903 |
| immunity to experimental neosporosis in pregnant sheep. | neospora caninum is an important cause of fetal loss in cattle but has also infrequently been shown to cause disease in sheep and goats. experimental infection of pregnant sheep with n. caninum causes clinical and pathological changes very similar to those of neosporosis in cattle. an experiment in sheep was undertaken to examine whether infection with n. caninum before pregnancy conferred immunity to subsequent challenge with the parasite during pregnancy. primary inoculation of nc1 tachyzoites ... | 2001 | 11240899 |
| molecular comparison of the dense granule proteins gra6 and gra7 of neospora hughesi and neospora caninum. | neospora hughesi is a recently described apicomplexan parasite that has been associated with several cases of equine protozoal myeloencephalitis. the biology of this new parasite is just beginning to be defined. towards this understanding, we report important differences between the nucleotide and deduced amino acid sequences of the dense granule proteins gra6 and gra7 of n. hughesi and neospora caninum. this information can be used to differentiate the two species and contribute to further unde ... | 2001 | 11226451 |
| serological analysis of calves experimentally infected with neospora caninum: a 1-year study. | a serological study was conducted with calves experimentally infected with the protozoan parasite neospora caninum. the animals were inoculated with either a low or high dose of n. caninum tachyzoites and temperature responses monitored daily for the first 2 weeks after inoculation. blood samples were collected before inoculation, and at regular intervals thereafter for 1 year. serological analysis was achieved using an indirect fluorescent antibody test (ifat), an enzyme-linked immunosorbent as ... | 2001 | 11182233 |
| characterization of the oregon isolate of neospora hughesi from a horse. | neospora hughesi was isolated in cell cultures inoculated with homogenate of spinal cord from a horse in oregon. tachyzoites of this oregon isolate of n. hughesi were maintained continuously by cell culture passage and tachyzoites were infective to immunosuppressed mice. gamma interferon gene knockout (ko) mice injected with tachyzoites developed fatal myocarditis and numerous tachyzoites were seen in lesions. gerbils (meriones unguiculatus) inoculated with tachyzoites developed antibodies (> or ... | 2001 | 11318565 |
| specific detection of neospora caninum oocysts in fecal samples from experimentally-infected dogs using the polymerase chain reaction. | neospora caninum oocysts, passed in the feces of a definitive host (dog), were isolated, and genomic dna was extracted. a polymerase cahin reaction (pcr) targeting the n. caninum-specific nc 5 genomic sequence was performed using the isolated dna. a synthesized competitor molecule containing part of the nc 5 sequence was included in the assay as a check against false-negative pcr results and to quantify n. caninum oocyst dna in fecal samples. a standard curve of the ratio of fluorescence intensi ... | 2001 | 11318571 |
| comparison of the growth inhibitory effects of canine ifn-alpha, -beta and -gamma on canine cells infected with neospora caninum tachyzoites. | the growth inhibitory effects of recombinant canine interferon alpha (ifn-alpha), beta (ifn-beta) and gamma (ifn-gamma) were examined on madin-darby canine kidney cells infected with neospora caninum tachyzoites. the parasite growth was inhibited by all ifns in a dose-dependent manner. ifn-gamma inhibited the parasite growth with greater efficacy than ifn-alpha or ifn-beta. moreover, the effect of ifns on n. caninum growth associated with the suppression of the host cell viability. the present s ... | 2001 | 11346181 |
| failure of dogs to shed oocysts after being fed bovine fetuses naturally infected by neospora caninum. | neospora caninum is a protozoan that causes abortion in cattle. the dog has recently been identified as a definitive host for n. caninum. to verify if bovine fetuses can infect dogs, nine 2-4-month-old dogs were fed bovine fetuses naturally infected by n. caninum. none of the dogs excreted oocysts, seroconverted, had clinical signs or lesions compatible with n. caninum infection. additional studies will be necessary to determine the natural mode of infection of dogs by n. caninum. | 2001 | 11358630 |
| neospora caninum infection associated with stillbirths in captive antelopes (tragelaphus imberbis). | neosporosis was diagnosed in full-term stillborn twin calves of captive antelopes (tragelaphus imberbis) in a german zoo. in both calves a multifocal non-suppurative encephalitis was present and infection with neospora (n.) caninum was confirmed by foetal serology and pcr. n. caninum-dna was detected in brain, heart, lung, liver and spleen of a third full-term stillborn calf. histopathological lesions were absent in this calf and antibodies against n. caninum were not detected in pleural fluid. ... | 2001 | 11358631 |
| prevalence of antibodies to neospora caninum and toxoplasma gondii in gray foxes (urocyon cinereoargenteus) from south carolina. | little is known about the epidemiology of neospora caninum in wild mammal populations. it has been suggested that a sylvatic cycle exists for n. caninum. dogs and potentially other canids are a definitive host for n. caninum. the present study was done to determine the prevalence of antibodies to n. caninum in a population of gray foxes (urocyon cinereoargenteus) from a nonagricultural setting in south carolina. we also determined the prevalence of antibodies to toxoplasma gondii in these animal ... | 2001 | 11358632 |
| reproductive performance of a cow-calf herd following a neospora caninum-associated abortion epidemic. | this study examines the long-term impact of a neospora caninum-associated abortion outbreak in a large cow-calf herd in northern alberta. blood samples were collected 4 times from all bred females and heifer calves born during the spring before the outbreak: (1) at the time of the outbreak, (2) the following spring, (3) the subsequent fall, and, finally, (4) the second spring after the outbreak. the samples were analyzed using a commercially available enzyme-linked immunosorbent assay for n. can ... | 2001 | 11360856 |
| dogs shed neospora caninum oocysts after ingestion of naturally infected bovine placenta but not after ingestion of colostrum spiked with neospora caninum tachyzoites. | an experiment was carried out to determine whether bovine colostrum or placenta could be a source of infection of neospora caninum for dogs. for this purpose, two dogs were fed bovine colostrum to which culture-derived n. caninum tachyzoites were added and two other dogs were fed placental cotyledonary tissue from n. caninum seropositive cows. one dog served as a negative control during the start of the experiment but this control dog was fed cotyledonary tissue later on. none of the dogs did pr ... | 2001 | 11403764 |
| myosin diversity in apicomplexa. | a polymerase chain reaction (pcr) screen was used to examine the diversity of myosins in 7 apicomplexan parasites: toxoplasma gondii, plasmodium falciparum, neospora caninum, eimeria tenella, sarcocystis muris, babesia bovis, and cryptosporidium parvum. using degenerate pcr primers compatible with the majority of known myosin classes, putative myosin sequences were obtained from all of these species. all of the sequences obtained showed greatest similarity to previously identified apicomplexan m ... | 2001 | 11318578 |
| experimental studies on the transmission of neospora caninum between cattle. | three studies were conducted to investigate the transmission of neospora caninum between cattle by the oral route. in the first study, six calves were dosed with 10(7)n caninum tachyzoites (nc livb1) in colostrum and/or milk replacer on four occasions. in the second study, two calves and two cows were fed placental tissues from n caninum -infected cows, and, in the third study, seven uninfected calves were fostered onto n caninum -infected dams. in the first study, all six calves developed antib ... | 2001 | 11356096 |
| seroprevalence of neospora caninum in female water buffaloes (bubalus bubalis) from the southeastern region of brazil. | antibodies to neospora caninum were assayed in sera of 222 female water buffaloes from ribeira valley of são paulo state, brazil, using an indirect fluorescent antibody test (ifat) and neospora agglutination test (nat). ifat antibodies were found in 64% of buffaloes with titers of 1:25 (42 buffaloes), 1:50 (53 buffaloes), 1:100 (31 buffaloes), 1:200 (10 buffaloes), 1:400 (3 buffaloes), or > or =1:800 (3 buffaloes). nat antibodies were found in 53% of buffaloes; in titers of 1:40 in 52 buffaloes, ... | 2001 | 11511420 |
| tgm2ap participates in toxoplasma gondii invasion of host cells and is tightly associated with the adhesive protein tgmic2. | like other members of the medically important phylum apicomplexa, toxoplasma gondii is an obligate intracellular parasite that secretes several classes of proteins involved in the active invasion of target host cells. proteins in apical secretory organelles known as micronemes have been strongly implicated in parasite attachment to host cells. tgmic2 is a microneme protein with multiple adhesive domains that bind target cells and is mobilized onto the parasite surface during parasite attachment. ... | 2001 | 11532123 |
| isolation and characterization of two parasitic protozoa from a pacific harbor seal (phoca vitulina richardsi) with meningoencephalomyelitis. | two species of protozoans were isolated from a harbor seal with fatal meninogoencephalitis. serologic reactivity was detected to both sarcocystis neurona and toxoplasma gondii. parasites associated with brain inflammation and necrosis reacted only with immunohistochemical stains utilizing polyclonal antisera raised against sarcocystis neurona. however, 2 distinct parasites were observed in cell cultures derived from the seal's brain tissue. these parasites were separated by mouse passage and lim ... | 2001 | 11534647 |
| evaluation of the embryo transfer procedure proposed by the international embryo transfer society as a method of controlling vertical transmission of neospora caninum in cattle. | to evaluate efficacy of embryo transfer into seronegative recipients, using the procedure proposed by the international embryo transfer society (iets), for preventing vertical transmission of neospora caninum in cattle. | 2001 | 11394835 |
| bovine abortion associated with neospora caninum in hungary. | the authors report the first case of bovine neosporosis in hungary based on investigations made on an aborted fetus. the diagnostic methods included traditional as well as molecular techniques. this record extends further the geographic range of the disease. | 2001 | 11402646 |
| detection of igg antibodies to neospora caninum and toxoplasma gondii in dogs examined in a veterinary hospital from brazil. | a total of 163 dogs with neuromuscular, respiratory and/or gastrointestinal disorders, was admitted at the veterinary hospital, federal university of uberlândia, brazil, and submitted to serology for toxoplasma gondii and neospora caninum. assays for t. gondii included indirect haemagglutination (iha), indirect fluorescent antibody (ifat-tg), immunoenzymatic (elisa), and immunoblotting (ib-tg). assays for n. caninum included ifat-nc and immunoprecipitation (ip-nc). based on concordant results by ... | 2001 | 11423182 |
| in the absence of endogenous gamma interferon, mice acutely infected with neospora caninum succumb to a lethal immune response characterized by inactivation of peritoneal macrophages. | following infection with neospora caninum, balb/c mice were shown to be resistant to an acute infection but developed a latent chronic infection. however, balb/c background gamma interferon (ifn-gamma)-deficient mice were sensitive to the acute infection. since the immune response in ifn-gamma-deficient mice is scantly known, we examined the function of macrophages, major histocompatibility complex (mhc) class ii expression, t-cell responses, and serum cytokine levels in the mice. all ifn-gamma- ... | 2001 | 11427432 |
| immunohistochemical and ultrastructural evidence for neospora caninum tissue cysts in skeletal muscles of naturally infected dogs and cattle. | protozoan stages were detected in the skeletal muscles of four dogs suffering from neosporosis and two neonatal calves with confirmed neospora caninum- infection which could be immunohistochemically labelled by an antiserum against the bradyzoite-specific antigen bag-5. in one calf, a tissue cyst was labelled by an antiserum against the n. caninum isolate nc-1. ultrastructurally, a 0.3-1 microm-thick cyst wall surrounded the labelled parasites. the cysts were located within myofibres and contain ... | 2001 | 11429181 |
| prevalence of neospora caninum antibodies in wild ruminants from the italian alps. | 2001 | 11442237 | |
| cutaneous neosporosis in two adult dogs on chronic immunosuppressive therapy. | antemortem diagnosis of generalized ulcerative and pyogranulomatous dermatitis with numerous intralesional tachyzoites was made from skin biopsy specimens from 2 adult dogs on chronic immunosuppressive therapy. a 9-year-old italian greyhound was on long-term corticosteroid therapy for the treatment of a lupus-like systemic autoimmune disorder, and a 7-year-old labrador retriever had received several months of chemotherapy for lymphosarcoma. the tachyzoites were identified as neospora caninum by ... | 2001 | 11482605 |
| first isolation of neospora caninum from the feces of a naturally infected dog. | neospora caninum is a major cause of abortion in cattle worldwide. cattle become infected with n. caninum by ingesting oocysts from the environment or transplacentally from dam to fetus. experimentally, dogs can act as definitive hosts, but dogs excrete few oocysts after ingesting tissue cysts. a natural definitive host was unknown until now. in the present study, n. caninum was isolated from the feces of a dog. gerbils (meriones unguiculatus) fed feces from the dog developed antibodies to n. ca ... | 2001 | 11426726 |
| genetic and biological diversity among isolates of neospora caninum. | neospora caninum is a protozoan parasite that causes bovine abortion. the epidemiology of n. caninum is poorly understood and little is known about the genetic diversity of the parasite, or whether individual isolates differ in virulence. such diversity may, among other factors, underlie the range of pathologies seen in cattle. in this study we analysed biological and genetic variation in 6 isolates of n. caninum originating from canine and bovine hosts by measurement of growth rate in vitro, we ... | 2001 | 11467779 |
| interferon-gamma-induced apoptosis in host cells infected with neospora caninum. | interferon-gamma (ifn-gamma) has a crucial role for host defence against parasite infection. it is not clear, however, how ifn-gamma affects the parasite-infected host cells. the effect of ifn-gamma on neospora caninum-infected cells was investigated in murine fibroblasts and canine kidney cells in vitro. in the presence of ifn-gamma, the viability of the infected host cell was decreased and apoptotic cell death occurred, as analysed by dna stainings with propidium iodide and a terminal deoxy-nu ... | 2001 | 11467780 |
| oocyst excretion in dogs fed mouse brains containing tissue cysts of a cloned line of neospora caninum. | neospora caninum is an apicomplexan parasite that causes neonatal neuromuscular disease in dogs and abortions in cattle. bovine neosporosis is a major production problem worldwide. the parasite is transmitted to cattle via oocysts excreted by dogs or by transplacental transmission. dogs are the only proven definitive host for n. caninum. one of 3 dogs fed mouse brains containing tissue cysts of a wild-type n. caninum strain ck0160sc3b (cko) excreted oocysts in its feces. two of 3 dogs fed mouse ... | 2001 | 11534658 |
| naturally-occurring neospora caninum infection in an adult sheep and her twin fetuses. | neospora caninum tissue cysts were found in the brains of surgically delivered twin fetuses at 119 days of gestation. in the brains of both fetuses, there was an inflammatory reaction involving perivascular cuffings of mononuclear cells, glial nodules. the dam of these fetuses died because of metritis. histopathological examination of the ewe revealed n. caninum tissue cysts and focal gliosis with mononuclear cell cuffings. a n. caninum-specific dna fragment was detected in a brain homogenate of ... | 2001 | 11318580 |
| prevalence of neospora caninum infection in dogs from beef-cattle farms, dairy farms, and from urban areas of argentina. | prevalence of anti-neospora caninum antibodies was determined in sera of 320 dogs from argentina using the indirect fluorescent antibody test (ifat). antibodies to n. caninum were found in 121 of 320 (37.8%) sera with titers of 1:50 (21 dogs), 1:100 (23 dogs), 1:200 (23 dogs), 1:400 (17 dogs), 1:800 (23 dogs), and > or = 1:1.600 (14 dogs). the seropositivity (ifat, > or = 1:50) was higher in dogs from dairy (48% of 125) and beef (54.2% of 35) farms than in dogs from urban areas (26.2% of 160). p ... | 2001 | 11534656 |
| association between exposure to neospora caninum and milk production in dairy cows. | to determine association between exposure to neospora caninum and milk production in dairy cows. | 2001 | 11549092 |
| leishmania infantum and neospora caninum simultaneous skin infection in a young dog in italy. | leishmania infantum, the agent of canine leishmaniasis in mediterranean countries, and neospora caninum, a recently recognized protozoal pathogen in dogs, were diagnosed in a 9-month-old argentine dogo dog. both skin lesions and neurological signs were present. histopathology of cutaneous lesions revealed a suppurative, diffuse dermatitis with numerous intracellular protozoa. serology was positive for both l. infantum (1:640) and n. caninum (1:800). double-label immunohistochemical staining of s ... | 2001 | 11705654 |
| bovine neosporosis: immunoblot improves foetal serology. | the purpose of the study is the comparative evaluation of the immunofluorescent antibody test (ifat) and an immunoblot (ib) test for the examination of foetal fluids for specific antibodies against neospora caninum. peritoneal and pleural fluids as well as abomasal contents were analysed. the results of the serological examinations were compared to those obtained by histological, immunohistochemical, and pcr analysis of foetal tissues as well as to the results of maternal serological examination ... | 2001 | 11731071 |
| isolation of neospora caninum from the brain of a naturally infected dog, and production of encysted bradyzoites in gerbils. | neospora caninum was isolated from the brain of an adult dog in brazil. cerebral tissue from the dog was inoculated into mongolian gerbils. gerbils were euthanized 3-4 months later and bradyzoite-containing tissue cysts were observed in their brains. n. caninum (designated nc-bahia) was isolated in cell culture after inoculation with tissue cysts from the gerbils. the identity of the parasite was confirmed by immunohistochemical examination and polymerase chain reaction (pcr). gerbils may be a u ... | 2001 | 11587828 |
| seroprevalences of toxoplasma gondii and neospora caninum in swedish red foxes (vulpes vulpes). | the prevalences of antibodies to the protozoan parasites toxoplasma gondii and neospora caninum were investigated by the direct agglutination test (dat) and elisa, respectively, in 221 red foxes (vulpes vulpes) from different parts of sweden. a total of 84 (38%) of the analysed sera had antibodies to t. gondii, but none of the foxes had antibodies to n. caninum. the results indicate that t. gondii infection is fairly common in swedish red foxes and that the infection is present in most parts of ... | 2001 | 11705663 |
| neospora caninum microneme protein ncmic3: secretion, subcellular localization, and functional involvement in host cell interaction. | in apicomplexan parasites, host cell adhesion and subsequent invasion involve the sequential release of molecules originating from secretory organelles named micronemes, rhoptries, and dense granules. microneme proteins have been shown to be released at the onset of the initial contact between the parasite and the host cell and thus mediate and establish the physical interaction between the parasite and the host cell surface. this interaction most likely involves adhesive domains found within th ... | 2001 | 11553593 |
| determination of antigenic domain in gst fused major surface protein (nc-p43) of neospora caninum. | the antigenic domain of the major surface protein (nc-p43) of neospora caninum was examined by polymerase chain reaction of its gene fragments and recombinant expression as gst fusion proteins. the fragments of nc-p43 were as follow: a total open reading frame (ofr), t; ofr without signal sequence and c-terminal hydrophobic sequence, s; n-terminal 2/3 parts of s, a; c-terminal 2/3 parts, p; n-terminal 1/3 part, x; middle 1/3 part, y; and c-terminal 1/3 part, z, respectively. the dna fragments we ... | 2001 | 11590914 |
| protection against vertical transmission in bovine neosporosis. | in this study we were interested to determine whether infection of cattle prior to pregnancy would afford any protection to the foetus if the dams were challenged with neospora caninum at mid-gestation. the experiment comprised four groups of cattle: group 1, uninfected controls; group 2, inoculated with n. caninum tachyzoites 6 weeks prior to mating and then challenged with n. caninum at mid-gestation; group 3, naive cattle challenged with n. caninum at mid-gestation and group 4 were infected w ... | 2001 | 11595240 |
| validation of a commercially available monoclonal antibody-based competitive-inhibition enzyme-linked immunosorbent assay for detection of serum antibodies to neospora caninum in cattle. | a previously described monoclonal antibody (mab)-based competitive-inhibition enzyme-linked immunosorbent assay (celisa) was modified to optimize performance, and the assay was validated in various defined cattle populations for detection of serum antibody to neospora caninum, a major cause of bovine abortion. modifications to the celisa included capturing native n. caninum antigen with a parasite-specific mab (mab 5b6-25) and directly conjugating the competitor mab (mab 4a4-2), with both mabs b ... | 2001 | 11682497 |
| protease activity and host cell binding of the 42-kda rhoptry protein from toxoplasma gondii after secretion. | three proteases were identified in the excretory/secretory proteins (esp) from toxoplasma gondii by the gelatin acrylamide gel electrophoresis (gage), of which the molecular masses were 80, 70, and 42 kda. one of the proteases with 42 kda was reactive to a monoclonal antibody (mab), tg786 clone, which was localized in the rhoptry of t. gondii by immunohistochemistry. the protease was maximally active at the ph range between 7.5 and 8.5, and was sensitive to inhibition by tpck and egta. the gelat ... | 2001 | 11563841 |
| characterization of neospora caninum surface protein ncsrs2 based on baculovirus expression system and its application for serodiagnosis of neospora infection. | the baculovirus expression system has proved to be a useful tool for the production of recombinant proteins. here we have characterized the neospora caninum surface protein ncsrs2 produced by two types of the recombinant virus and also have developed an enzyme-linked immunosorbent assay (elisa) using recombinant ncsrs2 for the serologic diagnosis of neospora infection. western blot analysis showed two major protein bands that were detectable in insect cells infected with each recombinant baculov ... | 2001 | 11682519 |
| ranch-management factors associated with antibody seropositivity for neospora caninum in consignments of beef calves in texas, usa. | a study was conducted with a 1998 retained-ownership population of texas (usa) beef calves to determine the ranch-management practices associated with calf seroprevalence to neospora caninum. management practices of 76 texas ranches that consigned 760 calves to a retained-ownership feedlot program were reviewed from a mailed questionnaire. ninety-nine of 760 (13%; 95% ci, 9.4%, 17.7%) calves were positive to n. caninum and 59% of the ranches consigned at least one positive calf. in the logistic ... | 2001 | 11566378 |
| epidemiological pattern and risk factors associated with bovine viral-diarrhoea virus (bvdv) infection in a non-vaccinated dairy-cattle population from the asturias region of spain. | a survey of bovine viral-diarrhoea virus (bvdv) infection was carried out in a non-vaccinated cattle population from the asturias region of spain in 1997 to assess seroprevalence and identify risk factors associated with infection. twenty-eight herds were included; 529 cows were bled. information regarding the herd and each animal sampled were recorded through a personal interview with the farmer. the true prevalence was estimated to be 21%. according to the antibody-age profiles and the herd-ma ... | 2001 | 11566379 |
| seroprevalence study of bovine neosporosis in mexico. | an indirect enzyme-linked immunosorbent assay was used to obtain epidemiologic information on bovine neosporosis in dairy herds of the mexican central plateau. sera were collected from 1,003 cows from 50 dairy herds. forty-three herds (group a) had been experiencing a high abortion rate. the abortion rates for the remaining 7 herds (group b) were within normal limits for mexico. five-hundred sixty-one (56%) of the 1,003 sera were positive. the seroprevalence of neospora caninum antibodies was 72 ... | 2001 | 11580063 |
| neosporosis in bovine dairy herds from the west of france: detection of neospora caninum dna in aborted fetuses, seroepidemiology of n. caninum in cattle and dogs. | neospora caninum is considered one of the major causes of abortion in cattle in most parts of the world. in this study, the role of n. caninum was investigated in groups of aborted cattle and dairy herds from the west of france. good correlation was found between parasite dna detection in fetuses and serologic statuses of dams. in groups with documented abortion status and no antibodies to other pathogens, 17-45% of aborted animals were seropositive for n. caninum, and significant relationship b ... | 2001 | 11731070 |
| isolation of neospora caninum from the brain of a pregnant sheep. | neospora caninum was isolated from the brain of a naturally infected pregnant sheep by inoculation of immunodeficient mice with a homogenate of the brain tissue. the ewe showed no clinical signs. tachyzoites were observed in the tissues of the nu/nu mice injected with the brain tissue homogenate and the diagnosis was confirmed by immunohistochemical staining with anti-n. caninum antibodies and by detecting n. caninum-specific dna by polymerase chain reaction. | 2001 | 11780846 |
| serological diagnosis of neosporosis in a herd of dairy cattle in southern brazil. | neospora caninum-specific antibodies were detected in 60 of 172 (34.8%) dairy cattle by enzyme-linked immunosorbent assay (elisa) in a herd from parana state, brazil. the seropositive animals included 47 of 126 (37.3%) adult cows, 7 of 29 (24%) heifers (1-2 yr), 4 of 15 (27%) heifers (5 mo-1 yr), and 2 precolostral samples. data collected over a 9-yr follow-up period revealed that the proportion of pregnancies ending in abortion was 20% (31/154) among elisa seropositive cows and 8% (15/193) amon ... | 2001 | 11780849 |