Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| vaccination of mice against experimental neospora caninum infection using ncsag1- and ncsrs2-based recombinant antigens and dna vaccines. | ncsag1 and ncsrs2, the two major immunodominant tachyzoite surface antigens of the apicomplexan parasite neospora caninum, were investigated for their potential as vaccine candidates in mice. recombinant recncsrs2 and recncsag1 were expressed in escherichia coli as poly-histidine-tagged fusion proteins. separate groups of mice were immunized with purified recncsag1, recncsrs2, or a combination of both, and were then challenged with n. caninum tachyzoites. subsequent experiments included intramus ... | 2003 | 12741509 |
| determination and correlation of anti-neospora caninum antibodies in dogs and cattle from mexico. | the aim of the present study was to determine and to compare through an indirect enzyme linked immunosorbent assay (elisa) test, the presence of anti-neospora caninum antibodies in city and farm dogs, as well as in farm cows, and the relationship among them. the correlation between anti-n. caninum antibodies in farm dogs and cattle was also assessed. the research was conducted in the dairy region of tizayuca, hidalgo, mexico. the frequency of anti-n. caninum antibodies was significantly higher i ... | 2003 | 12760481 |
| neospora caninum-infected cattle develop parasite-specific cd4+ cytotoxic t lymphocytes. | cattle infected with neospora caninum readily experience transplacental parasite transmission, presumably after maternal parasitemia, leading to abortion or birth of congenitally infected calves. cytotoxic t lymphocytes (ctl) are important mediators of protective immunity against toxoplasma gondii, an intracellular apicomplexan protozoan closely related to n. caninum. in this study, n. caninum-specific ctl expanded from peripheral blood mononuclear cells of two major histocompatibility complex-m ... | 2003 | 12761108 |
| the effect of the dam-calf relationship on serostatus to neospora caninum on 20 costa rican dairy farms. | an epidemiological study was conducted on 20 dairy herds previously diagnosed as seropositive for neospora caninum. the number of females per farm varies from 41 to 296. all females present on the farms were bled once in the period of july and august 2000. a total of 3002 females were bled. an indirect elisa was used to determine the serostatus of the animals. the analysis of the data was performed in four steps: (1) descriptive statistics about the serological status and general characteristics ... | 2003 | 12788252 |
| serological evidence of neospora caninum infections in beef bulls in six counties of the corrientes province, argentina. | the purpose of the present work was to identify neospora caninum infections in beef bulls belonging to 19 herds from six counties located in the corrientes province, argentina. the presence of antibodies to n. caninum was evaluated in 305 serum samples of bulls (bos taurus and bos indicus). age and breed were recorded. an indirect fluorescent antibody test was used to determine specific antibodies. the number of bulls with natural neospora-infection was 15 of 305 (4.9%). no association between s ... | 2003 | 12809751 |
| analysis of the sarcocystis neurona microneme protein snmic10: protein characteristics and expression during intracellular development. | sarcocystis neurona, an apicomplexan parasite, is the primary causative agent of equine protozoal myeloencephalitis. like other members of the apicomplexa, s. neurona zoites possess secretory organelles that contain proteins necessary for host cell invasion and intracellular survival. from a collection of s. neurona expressed sequence tags, we identified a sequence encoding a putative microneme protein based on similarity to toxoplasma gondii mic10 (tgmic10). pairwise sequence alignments of snmi ... | 2003 | 12814647 |
| electron microscopic study of in vitro cultures of hammondia heydorni (berlin 1996) tachyzoites after passages through dogs, mice, rats, guinea pigs and jirds. | this study describes the fine structure and reproduction of tachyzoites of hammondia heydorni (isolate berlin 1996) after in vitro cultivation of stages isolated from serial passages between dogs and various intermediate hosts such as mice, rats, guinea pigs, jirds, knock-out mice, goats and sheep. the tachyzoites are identical with respect to their ultrastructural features to the corresponding stages of the nc-1-strain of neospora caninum. this fact conforms with other already published data an ... | 2003 | 12838414 |
| prevalence of antibodies to neospora caninum in dogs from amazon, brazil. | neospora caninum is an important cause of abortion in dairy cattle worldwide. dogs are important in the epidemiology of this parasite because they are the only hosts known to excrete n. caninum oocysts. antibodies to n. caninum were assayed in serum samples from 157 dogs from monte negro, rondônia, amazon, brazil using the indirect fluorescent antibody test. antibodies to n. caninum were found in 13 (8.3%) of dogs in titers of 1:50 in 1, 1:100 in 2, 1:200 in 5, 1:800 in 1, 1:1600 in 2, and 1:320 ... | 2003 | 12860070 |
| antibodies to neospora caninum in european brown hare (lepus europaeus). | neospora caninum antibodies in european brown hares (lepus europaeus) were reported for the first time in sera collected from hares imported in italy from east europe. sera from 93 hares coming from hungary and 44 hares coming from slovakia were tested using a direct agglutination test (dat). seroprevalence was 8.0% at the 1:40 dilution and 1.5% at 1:320 dilution. seroprevalence in females and males and in different countries of origin did not differ significantly. our results indicate that euro ... | 2003 | 12860071 |
| characterization of an alpha tubulin gene sequence from neospora caninum and hammondia heydorni, and their comparison to homologous genes from apicomplexa. | the gene coding for a tubulin has been isolated by the polymerase chain reaction and sequenced from 2 isolates of neospora caninum (nc-liverpool and nc-sweb1). the data show that the gene, as in toxoplasma gondii, is single copy and contains 3 exons and 2 introns and is identical in sequence in the 2 isolates studied. comparison of the predicted protein sequence shows it to be identical to the a tubulin protein encoded by the t. gondii gene. the majority of the nucleotide substitutions that have ... | 2003 | 12866794 |
| neospora caninum antigens recognized by mouse igg at different stages of infection including recrudescence. | western blotting was performed to analyze neospora caninum tachyzoite antigens recognized by mouse igg at different stages of infection including recrudescence. at the early stage of infection, a 36-38 kda antigen was clearly recognized by the mouse antisera. after day 48 postinoculation, the signal of the 36-38 kda antigen gradually weakened. meanwhile, a 43 kda antigen was intensely and continuously recognized from 48 to 125 days postinoculation. this 43 kda antigen was clearly detectable with ... | 2003 | 12867739 |
| immunization of mice with plasmid dna coding for ncgra7 or ncshsp33 confers partial protection against vertical transmission of neospora caninum. | the purpose of the present study was to use direct plasmid deoxyribonucleic acid (dna) injection to identify specific antigens that confer protection against congenital transfer of neospora caninum. inbred balb/c mice were vaccinated before pregnancy with a recombinant plasmid containing sequences encoding n. caninum antigen ncgra7 or ncshsp33. the mice were challenged with n. caninum tachyzoites at 10-12 days of gestation. whereas 100% of pups born from dams immunized with control plasmid conta ... | 2003 | 12880248 |
| hammondia heydorni from the arabian mountain gazelle and red fox in saudi arabia. | unsporulated oocysts were detected in the feces of an arabian red fox (vulpes vulpes arabica) between 6 and 8 days after it had been fed meat from arabian mountain gazelles (gazella gazella) known to contain sarcocysts. no oocysts were discovered in the feces of other experimental cubs, although sporocysts of sarcocystis spp. were passed subsequently by all cubs that were fed gazelle meat, including those fed with reem (g. subgutturosa marica). the oocysts sporulated in 3 days at room temperatur ... | 2003 | 12880254 |
| protracted shedding of oocysts of neospora caninum by a naturally infected foxhound. | feces from 15 dogs at 2 different foxhound kennels in the u.k. were examined microscopically for the presence of oocysts of neospora caninum. one sample containing approximately 400 candidate oocysts per gram was positive in a polymerase chain reaction (pcr) using n. caninum-specific primers. in a sample taken 4 mo later from the same hound. n. caninum oocysts were again detected visually and by pcr. this is the third reported case of a dog naturally excreting oocysts of n. caninum and suggests ... | 2003 | 12880273 |
| the pathogenesis of neosporosis in pregnant cattle: inoculation at mid-gestation. | to investigate the pathogenesis of bovine neosporosis, 14 pregnant cattle were each inoculated subcutaneously with either 10(7) or 5 x 10(8) neospora caninum (strain nc1) tachyzoites at 140 days' gestation. serial necropsies were then carried out over an 8-week period. in the placenta, neospora dna and histopathological changes were observed in samples taken 14 days post-inoculation (dpi), with focal necrosis of maternal caruncular septa and fetal placental villi, serum leakage, and a maternal a ... | 2003 | 12921725 |
| seroprevalence of neospora caninum in aborting dairy cattle in the czech republic. | a serological survey for antibodies against neospora caninum in aborting cattle was carried out in the czech republic. serum samples from 463 aborting dairy cows originated from 137 farms from different parts of the czech republic were tested for presence of n. caninum antibodies by use of an enzyme-linked immunosorbent assay (elisa) and an indirect fluorescent antibody test (ifat). antibodies (> or = 1:640) to n. caninum were found in 18 (3.9%) of 463 aborting cows. farm prevalence in aborting ... | 2003 | 12935739 |
| development and evaluation of a seminested pcr for detection and differentiation of babesia gibsoni (asian genotype) and b. canis dna in canine blood samples. | canine babesiosis has recently been recognized as an emerging infectious disease of dogs in north america. we sought to develop a seminested pcr to detect and differentiate babesia gibsoni (asian genotype), b. canis subsp. vogeli, b. canis subsp. canis, and b. canis subsp. rossi dna in canine blood samples. an outer primer pair was designed to amplify an approximately 340-bp fragment of the 18s rrna genes from b. gibsoni (asian genotype), b. canis subsp. vogeli, b. canis subsp. rossi, and b. can ... | 2003 | 12958243 |
| laboratory passage and characterization of an isolate of toxoplasma gondii from an ocular patient in korea. | toxoplasma gondii tachyzoites were isolated from the blood of an ocular patient, and have been successfully passaged in the laboratory, for over a year, by peritoneal inoculation in mice. the isolated parasite was designated the korean isolate-1 (ki-1) and its characteristics were compared with those of the rh strain, a wellknown virulent strain originating from a child who suffered from encephalitis. the morphology, pathogenicity, infectivity and cell culture characteristics of the ki-1 were si ... | 2003 | 12972728 |
| elisa detection of igg antibody against a recombinant major surface antigen (nc-p43) fragment of neospora caninum in bovine sera. | an elisa was established to measure bovine igg directed against the recombinant antigenic determinant of nc-p43, a major surface antigen of neospora caninum. in a previous study, two thirds of the c-terminal of the molecule was expressed as a 6 x his tagged protein (ncp43p) for elisa using 2/3 of the n-terminal of sag1 from toxoplasma gondii as a control (tgsag1a). among 852 cattle sera collected from stock farms scattered nation-wide, 103 sera (12.1%) were found to react with ncp43p positively, ... | 2003 | 12972732 |
| first demonstration of protective immunity against foetopathy in cattle with latent neospora caninum infection. | the parasite neospora caninum is an important cause of abortion in cattle world-wide. chronically infected dams transmit the parasite transplacentally and infected foetuses may be aborted or born chronically infected but clinically normal. chronically infected cows repeatedly transmit the parasite to foetuses in several pregnancies and some may abort more than once suggesting that the immune response in these cattle is compromised during pregnancy. to investigate the nature of the immune respons ... | 2003 | 13129528 |
| aetiology of bovine abortion in argentina. | necropsies were performed on 354 fetuses from dairy and beef herds submitted from 1994 to 2000 to the diagnostic laboratories at instituto nacional de tecnología agropecuaria, balcarce, argentina. samples from the fetuses were examined for pathogenic organisms and processed for microscopic examination. an aetiological diagnosis was made for 161 (45.5%) of the fetuses. no diagnosis was made for 193 (54.5%) fetuses. infectious agents were isolated from 122 (34.4%) of the fetuses, bacterial agents ... | 2003 | 14509450 |
| anti-protozoal efficacy of medicinal herb extracts against toxoplasma gondii and neospora caninum. | the purpose of this study was to determine whether alcohol extracts of herbs (sophora flavescens aiton, sinomenium acutum (thunb.) rehder and e.h. wilson, pulsatilla koreana (yabe ex nakai) nakai ex t. mori, ulmus macrocarpa hance and torilis japonica (houtt.) dc.) from south korea, possess in vitro anti-protozoal activity against cultures of toxoplasma gondii and neospora caninum. these herbs have been used as human anti-parasitics in asian countries for many years. alcohol extracts of these he ... | 2003 | 14519322 |
| dermatitis in a dog associated with an unidentified toxoplasma gondii-like parasite. | protozoal dermatitis was diagnosed in a 6-year-old female great dane dog from rio de janeiro, brazil. the dog died because of a chronic illness with an ehrlichia-like organism. numerous apicomplexan parasites were identified histologically in the section of dermal lesions. the protozoan reacted with toxoplasma gondii polyclonal rabbit serum but not with neospora caninum or sarcocystis neurona antibodies. ultrastructurally, the protozoa was not t. gondii because it had schizont-like structures wi ... | 2003 | 14519327 |
| cytokine gene expression in dams and foetuses after experimental neospora caninum infection of heifers at 110 days of gestation. | neospora caninum is a major cause of abortion in cattle. an essential role for th1 cytokines, such as ifn-gamma and il-12 in protective immunity against n. caninum in murine models has been indicated. however, little is known about immunity to neospora in pregnant cattle where a considerable level of immunomodulation may exist. in this study, the immune response of heifers infected early in the second trimester of pregnancy by intravenous inoculation of n. caninum tachyzoites was compared with i ... | 2003 | 14521581 |
| prevalence of antibodies to neospora caninum, sarcocystis neurona, and toxoplasma gondii in wild horses from central wyoming. | sarcocystis neurona, neospora caninum, n. hughesi, and toxoplasma gondii are 4 related coccidians considered to be associated with encephalomyelitis in horses. the source of infection for n. hughesi is unknown, whereas opossums, dogs, and cats are the definitive hosts for s. neurona, n. caninum, and t. gondii, respectively. seroprevalence of these coccidians in 276 wild horses from central wyoming outside the known range of the opossum (didelphis virginiana) was determined. antibodies to t. gond ... | 2003 | 14533680 |
| meningoencephalitis associated with an unidentified apicomplexan protozoan in a pacific harbor seal. | a pacific harbor seal (phoca vitulina richardsii) was found on the central california coast with neurologic signs and labored breathing, which were unresponsive to treatment. necropsy revealed a nonsuppurative necrotizing meningoencephalitis, a multilocular thymic cyst, and nonsuppurative cystitis and renal pyelitis. microscopic examination revealed protozoans in the brain, thymic cyst, and bladder mucosa. ultrastructurally, the protozoal tachyzoites were different from those of neospora caninum ... | 2003 | 14533706 |
| autofluorescence of toxoplasma gondii and related coccidian oocysts. | this is the first report of blue autofluorescence as a useful characteristic in the microscopic detection of toxoplasma gondii, hammondia hammondi, hammondia heydorni, neospora caninum, besnoitia darlingi, and sarcocystis neurona oocysts or sporocysts. this autofluorescence is of sufficient intensity and duration to allow identification of these oocysts from complex microscopic sample backgrounds. as with the autofluorescence of related coccidia, the oocysts glow pale blue when illuminated with ... | 2003 | 14533708 |
| seroprevalence of neospora caninum and toxoplasma gondii antibodies in the south american opossum (didelphis marsupialis) from the city of são paulo, brazil. | antibodies to neospora caninum and toxoplasma gondii were assayed in sera of 396 opossums (didelphis marsupialis) from the city of são paulo, brazil. antibodies to n. caninum were assayed using the indirect immunofluorescent antibody test (ifat). antibodies (ifat, approximately 1:25) to n. caninum were found in 84 opossums (d. marsupialis) in titers of 1:25 in 46, 1:50 in 20, 1:100 in 17, and 1:400 in 1. antibodies to t. gondii were assayed with the modified agglutination test (mat) and the ifat ... | 2003 | 14533710 |
| isolation of neospora caninum from a blind calf in paraná, southern brazil. | 2003 | 14533770 | |
| neospora caninum and toxoplasma gondii: a novel adhesion/invasion assay reveals distinct differences in tachyzoite-host cell interactions. | this paper describes an adhesion/invasion assay, based on combined pyrrolidine dithiocarbamate (pdtc) and antibody treatment of parasites followed by quantitative real-time pcr. this pdtc-pcr assay can be used to comparatively assess the participation of host cell- and parasite-associated components during host cell adhesion and entry by neospora caninum and toxoplasma gondii tachyzoites, respectively, and is potentially applicable to any other apicomplexan parasite. the assay allows to determin ... | 2003 | 14552862 |
| a role for balance of interferon-gamma and interleukin-4 production in protective immunity against neospora caninum infection. | a suitable balance in the production of th1/th2-type cytokines has a crucial role in the control of microbial infections. we investigated cytokine production patterns and effects during neospora caninum infection, based on two mouse models and an in vitro system. in the acute infection of n. caninum, balb/c-background ifn-gamma-deficient mice that were sensitive to the n. caninum infection showed high levels of il-10 production, whereas significant levels of interferon-gamma (ifn-gamma) and inte ... | 2003 | 14559161 |
| toxoplasma gondii, neospora caninum, sarcocystis neurona, and sarcocystis canis-like infections in marine mammals. | toxoplasma gondii, neospora caninum, sarcocystis neurona, and s. canis are related protozoans that can cause mortality in many species of domestic and wild animals. recently, t. gondii and s. neurona were recognized to cause encephalitis in marine mammals. as yet, there is no report of natural exposure of n. caninum in marine mammals. in the present study, antibodies to t. gondii and n. caninum were assayed in sera of several species of marine mammals. for t. gondii, sera were diluted 1:25, 1:50 ... | 2003 | 14580799 |
| seroprevalence of neospora caninum and toxoplasma gondii in captive and free-ranging nondomestic felids in the united states. | seven serum samples of 101 samples from nondomestic, captive and free-ranging felids from the united states were indirect fluorescent antibody positive for antibodies to neospora caninum, whereas 44 samples were positive for antibodies to t. gondii. although none of the captive animals displayed clinical signs of disease, nondomestic felids in the united states have been exposed to, and are likely infected with, n. caninum and t. gondii. this may have serious implications for zoological gardens ... | 2003 | 14582785 |
| humoral immune response to infectious agents in aborted bovine fetuses in argentina. | the purpose of this study was to characterize the exposure of bovine aborted fetuses from beef and dairy herds of the humid pampas of argentina to different infectious agents by the evaluation of fetal fluid antibodies. presence of fetal antibodies to bovine viral diarrhea virus genotype 1 (bvdv-1), bovine herpes virus type 1 (bhv-1), leptospira interrogans, brucella abortus, and neospora caninum was determined. of the 95 fetuses processed, 66 came from 49 beef herds and 29 from 12 dairy herds. ... | 2003 | 14587376 |
| detection of hammondia heydorni-like organisms and their differentiation from neospora caninum using random-amplified polymorphic dna-polymerase chain reaction. | neospora caninum and hammondia heydorni are morphologically and phylogenetically related coccidians that are found in dogs. new diagnostic genetic loci, based on random-amplified polymorphic dna-polymerase chain reaction (rapd-pcr), were developed to aid in the detection of h. heydorni-like parasites and to discriminate them from n. caninum and other related coccidians of dogs. on the basis of the data obtained from 5 random decamers, h. heydorni (manhattan-1) and n. caninum (nc1) were character ... | 2003 | 14627168 |
| reproductive losses due to neospora caninum in a beef herd in argentina. | reproductive losses in a beef herd of 857 heifers with a pregnancy rate of 86.3% are described. after pregnancy testing, 69 abortions were seen during a 3 month period. before calving season, three heifers had delivered pre-mature non-viable calves. serum samples from 58 of 69 aborted heifers were available for serological tests. in order to compare the seroprevalence in non-aborted vs. aborted heifers, 214 pregnant animals were bleed during the abortion storm. in addition, blood samples were co ... | 2003 | 14629003 |
| neospora caninum in sheep: a herd case report. | neospora caninum was detected by means of pcr in the brain of 4 out of 20 aborted fetuses in a flock of 117 sheep exhibiting a persistent abortion problem, and n. caninum tissue cysts were furthermore found in encephalitic lesions in one of the pcr-positive fetuses. toxoplasma gondii was detected as aborting agent in another 3 out of 20 fetuses. antibodies to n. caninum (by indirect fluorescence antibody test (ifat)) were found in 10.3% of 117 ewes and antibodies for t. gondii were found in 97.4 ... | 2003 | 14630429 |
| serological status and abortion rate in progeny obtained by natural service or embryo transfer from neospora caninum-seropositive cows. | bovine neosporosis is an important disease in dairy herds in argentina. embryo transfer (et) technology offers the opportunity to minimize vertical transmission of neosporosis. the objective of this work was to compare the serological status and the incidence of abortion between heifers obtained via natural service and heifers obtained by et from neospora-infected cows. progeny from eight neospora-infected cows belonging to a commercial dairy farm where et was routinely performed were studied. b ... | 2003 | 14633201 |
| neospora caninum infection and congenital transmission: serological and parasitological study of cows up to the fourth gestation. | in this paper, a parasitological and serological study performed in three cows up to the fourth gestation is reported in order to clarify the extent of vertical propagation and, secondly, in which period of gestation the recrudescence of previous infection occurs. the cows selected for the study delivered healthy but congenitally infected calves in first pregnancy. the parasite was found, by biological tests in swiss mice, in all the placentas of the three cows examined, during the three subsequ ... | 2003 | 14633211 |
| regional distribution of bovine neospora caninum infection in the german state of rhineland-palatinate modelled by logistic regression. | to obtain a rapid overview over the distribution of bovine neospora caninum-infections in the german state of rhineland-palatinate, an elisa to determine specific bovine antibodies against a p38 surface antigen of n. caninum tachyzoites was modified to examine bulk milk samples from cattle herds. experimental bulk milk samples were used to demonstrate that the seroprevalence in a group of animals can be estimated with this elisa. a cut-off was selected for the specific detection of herds having ... | 2003 | 14636679 |
| detection of neospora caninum in semen of bulls. | in cattle, transplacental infection is the main route of neospora caninum transmission, but postnatal transmission by the oral uptake of sporozoite-containing oocysts shed by dogs may also be possible. other routes of horizontal transmission, such as the venereal route, have not been investigated. in this study, we evaluated the presence of n. caninum dna by a nested-pcr in fresh non-extended semen and frozen extended semen straws of five holstein-friesian bulls with naturally-acquired neosporos ... | 2003 | 14637032 |
| a long-term study of neospora caninum infection in a swedish dairy herd. | a longitudinal study was performed in a swedish dairy herd where neospora caninum had been isolated from a stillborn calf. starting in autumn 1994, blood samples from all female animals in the herd were collected once yearly until 1999. the sera were analysed for presence of igg1 antibodies to n. caninum by the iscom elisa, and by an avidity elisa to establish the timing of infection. in addition, data on reproductive performance were compiled. during the study the percentage of seropositive fem ... | 2003 | 14650545 |
| investigation of neospora sp. antibodies in aborted mares from normandy, france. | neospora caninum, an apicomplexan protozoan parasite, is recognized as a major cause of abortion in cattle while limited information is presently available on association between equine neospora infections and abortions. the aim of the present study was to document prevalence of antibodies against neospora sp. in aborted mares as a clue to the role of n. caninum in mare reproductive failure in normandy, france. using an agglutination test, the number of animals with elevated (>80) anti-neospora ... | 2003 | 14651869 |
| seroprevalence and associated risk factors of neosporosis in beef and dairy cattle in italy. | a cross-sectional serological survey for neospora caninum was carried out on beef and dairy cattle in southern and northern italy. a total of 111 herds and 1140 animals were tested using an elisa assay (chekit-neospora) to detect anti-n. caninum antibodies. management and individual data were collected and analysed both by linear and logistic multiple-regression models in order to find good predictors of the cattle seroprevalence and anti-n. caninum antibody level. at least one seropositive anim ... | 2003 | 14651870 |
| antibodies to neospora caninum in wild animals from kenya, east africa. | the prevalence of antibodies to neospora caninum was examined in six wild artiodactyla species, and in five wild carnivora species from kenya. blood sera (104 wild ungulates from marula estates (mes), and 31 wild carnivores from masai-mara reserve and from other wildlife areas in northern and southern kenya), were screened using a neospora agglutination test (nat), with a twofold dilution (1:40-1:320 titres). presence of nat antibodies to n. caninun is reported here for the first time in zebra ( ... | 2003 | 14651874 |
| use of a multiplex polymerase chain reaction assay in the antemortem diagnosis of toxoplasmosis and neosporosis in the central nervous system of cats and dogs. | to develop a multiplex polymerase chain reaction (pcr) assay for the detection of toxoplasma gondii and neospora caninum dna in canine and feline biological samples. sample population; biological samples from 7 cats with systemic (n = 4) or cns (3) toxoplasmosis, 6 dogs with neospora- or toxoplasma-associated encephalitis, and 11 animals with nonprotozoal disease. | 2003 | 14672429 |
| establishment of a two-dimensional electrophoresis map for neospora caninum tachyzoites by proteomics. | expressed proteins and antigens from neospora caninum tachyzoites were studied by two-dimensional gel electrophoresis and immunoblot analysis combined with matrix-assisted laser desorption/ionization-time of flight mass spectrometry. thirty-one spots corresponding to 20 different proteins were identified from n. caninum tachyzoites by peptide mass fingerprinting. six proteins were identified from a n. caninum database (ntpase, 14-3-3 protein homologue, ncmic1, ncdg1, ncgra1 and ncgra2), and 11 p ... | 2003 | 14673785 |
| risk of abortion associated with neospora caninum during different lactations and evidence of congenital transmission in dairy cows. | to examine the relationship between exposure to neospora caninum and abortion in dairy cows during their first, second, third, and fourth or later lactations and to establish the main mode of transmission in female calves from birth until their first pregnancy was terminated by abortion or parturition. | 2002 | 12494973 |
| the influence of feeding and maintenance system on occurrence of toxoplasma gondii infections in dogs. | serum samples of 113 dogs visiting "outpatient clinics", 52 dogs kept in shelters and 35 animals from a military dog training centre were examined for toxoplasma gondii specific antibodies using a latex agglutination test. significant differences in seroprevalences were found between dogs from the training centre (8.6% of positive results) and the other populations examined (40.7% of positive seroreagents in animals visiting outpatient clinics and 44.2% in the group from shelters, respectively). ... | 2002 | 12512556 |
| diagnostic survey of bovine abortion in korea: with special emphasis on neospora caninum. | causes of bovine abortion were surveyed in korea within a designated period from the cases submitted to the department of veterinary pathology, college of veterinary medicine, seoul national university. one hundred and eighty aborted fetuses and maternal sera were evaluated by necropsy, histopathology, bacteriology, virology, pcr, and serologic tests. the causes of abortion were identified in 108 (60%) cases, of which 38 (21.1%) were due to the infection with neospora caninum. none of the 38 cas ... | 2002 | 12520105 |
| application of real-time fluorescent pcr for quantitative assessment of neospora caninum infections in organotypic slice cultures of rat central nervous system tissue. | the previously described nc5-specific pcr test for the diagnosis of neospora caninum infections was used to develop a quantitative pcr assay which allows the determination of infection intensities within different experimental and diagnostic sample groups. the quantitative pcr was performed by using a dual fluorescent hybridization probe system and the lightcycler instrument for online detection of amplified dna. this assay was successfully applied for demonstrating the parasite proliferation ki ... | 2002 | 11773124 |
| in contrast to dogs, red foxes (vulpes vulpes) did not shed neospora caninum upon feeding of intermediate host tissues. | to clarify whether red foxes (vulpes vulpes) can be final hosts of neospora caninum, foxes and dogs were fed in parallel on tissues of a sheep and a goat experimentally infected with n. caninum. the faeces of at least two of five dogs contained n. caninum oocysts, as determined by bioassay. in the faeces of all six foxes fed in parallel, oocysts were detected that were larger in size (length 12.6 +/- 0.5 microm, width 11.8 +/- 0.4 microm) than the oocysts shed by the dogs. ribosomal rna sequence ... | 2002 | 11822736 |
| neospora caninum and hammondia heydorni are separate species/organisms. | neospora caninum and hammondia heydorni are two coccidian parasites with morphologically similar oocysts in canine feces. it was recently proposed that they are one species. in this paper, we review the biology and morphology of these parasites and present evidence that n. caninum and h. heydorni are separate species. | 2002 | 11832296 |
| neospora 2001. | infection with the protozoan parasite neospora caninum is emerging as a major cause of reproductive loss in cattle worldwide. recent advances in research with the ultimate goal of devising effective and sustainable control measures were discussed at neospora 2001. neospora 2001 was held, 13-15 september 2001, at the moredun research institute, edinburgh, uk. the international meeting covered many aspects of bovine neosporosis including diagnosis, epidemiology and immunology. | 2002 | 11850000 |
| neospora caninum and waddlia chondrophila strain 2032/99 in a septic stillborn calf. | characteristics of an intracellularly growing micro-organism isolated from an aborted bovine foetus are described. the organism replicated within cytoplasmic vacuoles, was resistant to penicillin and exhibited structural characteristics compatible with waddlia chondrophila. an elisa specific for chlamydia spp., immunofluorescence tests using antibodies directed against chlamydia spp. or simkania negevensis, and pcr using chlamydia-specific primers showed that the agent was distinct from chlamydi ... | 2002 | 11852195 |
| vaccine development against neospora caninum infection. | neospora caninum is a recognized protozoan parasite of a wide range of mammalian hosts, and was reported for the first time in 1988. the isolation of its oocysts in dog's faeces in 1998 led to its establishment as a parasitic species undergoing typical coccidian life cycle. infection with n. caninum causes paralysis and death in young livestock and companion animals, and is associated with abortions and stillbirth in cattle, and neurologic disease in calves. considering the economic and agricult ... | 2002 | 11853138 |
| neosporosis with cerebellar involvement in an adult dog. | a case of an adult dog with multifocal, progressive neurological signs caused by neospora caninum is reported. pathological studies showed cerebellar lesions due to the parasite, which was also present in other parts of the nervous system and muscle. cerebellar atrophy related to neospora infection has been rarely reported in veterinary medicine, and has been shown to affect ruminants and dogs. the cerebellar involvement and the age of the present dog make this case uncommon. | 2002 | 11873953 |
| point source exposure of cattle to neospora caninum consistent with periods of common housing and feeding and related to the introduction of a dog. | eight dairy herds with evidence of post-natal transmission of neospora caninum were used to test the hypothesis of a point source exposure by a retrospective analysis of the housing and feeding of infected age-groups. the first n. caninum-associated abortion or birth of n. caninum-seropositive offspring from the post-natally infected age-group was considered as the first indication of the infection. in seven of the eight dairy herds, a point source exposure to n. caninum of the infected age-grou ... | 2002 | 11900922 |
| natural transmission routes of neospora caninum between farm dogs and cattle. | twelve dairy herds with evidence of post-natal infection with neospora caninum were compared with 21 control herds with no evidence of post-natal infection. on the former farms, dogs consumed placenta or licked uterine discharge in 75 and 67% of the farms, respectively, while on control farms these activities occurred in 38 and 24% of the farms, respectively. on all control farms and all but three post-natally infected farms the dogs were fed colostrum or milk. defecation of dogs on the feeding ... | 2002 | 11900923 |
| a method of probability diagnostic assignment that applies bayes theorem for use in serologic diagnostics, using an example of neospora caninum infection in cattle. | to develop a method of probability diagnostic assignment (pda) that uses continuous serologic measures and infection prevalence to estimate the probability of an animal being infected, using neospora caninum as an example. | 2002 | 11911564 |
| studies on serological cross-reaction of neospora caninum with toxoplasma gondii and hammondia heydorni. | the enzyme-linked immunosorbent assay (elisa) was used to examine cross-reactivity of neospora caninum with toxoplasma gondii and hammondia heydorni. anti-t. gondii mouse and cat sera cross-reacted with n. caninum soluble antigen (nla), but not with the recombinant surface antigen (ncsrs2). anti-h. heydorni dog sera showed no cross-reactivity with either the nla antigen or the ncsrs2. lack of cross-reactivity between anti-h. heydorni sera and n. caninum antigens, and the cross-reactivity of anti ... | 2002 | 11913555 |
| effect of neospora caninum on in vitro development of preimplantation stage bovine embryos and adherence to the zona pellucida. | 2002 | 11913589 | |
| necrotizing cerebellitis due to neospora caninum infection in an old dog. | a severe, necrotizing, non-suppurative inflammation of the cerebellum associated with neospora caninum infection was identified in a 14-year-old male labrador retriever. on presentation, clinical signs included mild depression and head tremor, marked ataxia of both thoracic and pelvic limbs, and abnormal postural reactions. in the central nervous system, inflammatory lesions were mainly restricted to the cerebellar leptomeninges and cerebellar cortex, which appeared necrotic and atrophic. protoz ... | 2002 | 11913826 |
| quantitative detection of neospora caninum in bovine aborted fetuses and experimentally infected mice by real-time pcr. | we report the development of a real-time pcr assay for the quantitative detection of neospora caninum in infected host tissues. the assay uses the double-stranded dna-binding dye sybr green i to continuously monitor product formation. oligonucleotide primers were designed to amplify a 76-bp dna fragment corresponding to the nc5 sequence of n. caninum. a similar method was developed to quantify the 28s rrna host gene in order to compare the parasite load of different samples and to correct for th ... | 2002 | 11923330 |
| neosporosis as a cause of abortion in dairy cattle in rio grande do sul, southern brazil. | forty-six aborted bovine fetuses submitted to the faculty of veterinary, department of clinical pathology, universidade federal do rio grande do sul, were examined histopathologically. non-suppurative inflammation was observed mainly in the brain and heart of 22 fetuses. brain lesions consisted primarily of mononuclear inflammatory cell infiltrates with occasional foci of necrosis. protozoa that reacted with neospora caninum antisera were seen in 18 of the 22 (81.8%) brain specimens from fetuses ... | 2002 | 11750112 |
| in vitro antiprotozoal effects of artemisinin on neospora caninum. | neospora caninum is an intracellular apicomplexan parasite that infects a wide range of mammals and has been associated with abortion in cattle worldwide. artemisinin is an effective antimalarial compound derived from a traditional chinese herbal remedy, qinghao or artemisia annua l. in the study reported, the cultured host cells (vero cells or mouse peritoneal macrophages) infected with n. caninum tachyzoites were incubated with alpha-mem (minimal essential medium) 10%hs supplemented with vario ... | 2002 | 11751000 |
| seroprevalence of antibodies against neospora caninum in diagnostic equine serum samples and their possible association with fetal loss. | a case-control study of the association between the presence of serum antibodies against neospora spp. and fetal loss was performed on serum samples submitted to a veterinary diagnostic laboratory in northwestern united states. control sera were randomly selected from those submitted from healthy horses for routine equine infectious anemia testing required for regulatory health certification. case sera were randomly selected from those submitted from aborting mares for diagnostic workup. based o ... | 2002 | 11934465 |
| neospora caninum is an invalid species name: an evaluation of facts and statements. | an evaluation of both the formal requirements of the international rules of zoological nomenclature and the scientific reasons for the description of new genera and species shows that the name neospora caninum is a nomen nudum. the only characteristic criteria for discriminating between the previously described species hammondia heydorni and the proposed new species n. caninum (i.e. the lack of a parasitophorous vacuole) has been shown to be wrong in many publications. furthermore, absolutely no ... | 2002 | 11936509 |
| factors associated with neospora caninum serostatus in cattle of 20 specialised costa rican dairy herds. | twenty-five specialised costa rican dairy farms (located in the poás area) were used to determine neosporosis seroprevalence and the association of seropositivity with environmental and management factors. the farms involved were selected intentionally and all of them use vampp 5.1 (veterinary automated management and production control programme) as management-information system. holstein-friesian, jersey and crosses between them were the most-frequent breeds in these herds. the number of femal ... | 2002 | 11937233 |
| infection of organotypic slice cultures from rat central nervous tissue with neospora caninum: an alternative approach to study host-parasite interactions. | neospora caninum is an apicomplexan parasite which has emerged as an important cause of bovine abortion worldwide. abortion is usually triggered by reactivation of dormant bradyzoites during pregnancy and subsequent congenital infection of the foetus, where the central nervous system appears to be most frequently affected. we here report on an organotypic tissue culture model for neospora infection which can be used to study certain aspects of the cerebral phase of neosporosis within the context ... | 2002 | 11943226 |
| companion animal parasitology: a clinical perspective. | in recent years there have been many changes to the ways that clinical veterinary science is conducted and nowhere is this more evident than in companion animal practice. veterinarians working with pet dogs and cats are facing new challenges associated with the emergence and re-emergence of parasitic diseases. some, such as neospora caninum, have been recently recognised; others like giardia and cryptosporidium have been reported with increasing frequency, in part as a result of laboratory tests ... | 2002 | 11943231 |
| diagnosis and seroepidemiology of neospora caninum-associated bovine abortion. | a round table was conducted at the viiith international coccidiosis conference on neospora diagnosis with particular emphasis on strategies to diagnose bovine abortion. the strength and weakness of different assays for neospora caninum infection and whether these methods have resulted in the overdiagnosis of neosporosis was discussed. it was evident that each diagnostic method, namely histology, immunohistochemistry, molecular detection and serological assays were, under certain circumstances, v ... | 2002 | 11943234 |
| use of a serum-free medium to produce in vitro neospora caninum and toxoplasma gondii tachyzoites on vero cells. | neospora caninum and toxoplasma gondii are cyst-forming coccidian parasites of human and veterinary clinical relevance. in vitro cultivation of the protozoans using vero cells is usually performed in order to produce antigenic materials. quantitative and qualitative comparisons of vero cells grown in rpmi medium supplemented either with foetal calf serum (fcs), horse serum (hs) or a specific serum-free additive (defcell) were performed. a serum-free cell culture system used to propagate n. canin ... | 2002 | 11944805 |
| sensitive and specific identification of neospora caninum infection of cattle based on detection of serum antibodies to recombinant ncp29. | neosporosis is an economically important disease of dairy cattle caused by the protozoan neospora caninum. diagnostic tests for neosporosis are complicated by the potential for cross-reaction of antibodies to antigens that are similar between n. caninum and closely related parasites toxoplasma gondii and sarcocystis cruzi. to provide a sensitive and specific assay for detecting antibodies to n. caninum in the serum of infected animals, we have investigated a recombinant form of the antigen known ... | 2002 | 11986268 |
| immunological relationship between neospora caninum and besnoitia besnoiti. | neospora caninum is a coccidian parasite identified as a major cause of abortion in cattle. a combined infection of n. caninum with another taxonomically related parasite of cattle, besnoitia besnoiti can occur in geographical areas endemic for both species. both infections are routinely diagnosed serologically, and incorrect diagnosis could occur if immunological cross-reactivity exists between the two parasites. to investigate the possible degree of cross-reactivity, we compared results obtain ... | 2002 | 11992709 |
| identification of a neospora caninum microneme protein (ncmic1) which interacts with sulfated host cell surface glycosaminoglycans. | the invasive stages of apicomplexan parasites enter their host cells through mechanisms which are largely conserved throughout the phylum. host cell invasion is divided into two distinct events, namely, adhesion onto the host cell surface and the actual host cell entry process. the former is mediated largely through microneme proteins which are secreted at the onset of establishing contact with the host cell surface. many of the microneme proteins identified so far contain adhesive domains. we h ... | 2002 | 12011014 |
| epidemiological investigations of abortions due to neospora caninum on swiss dairy farms. | abortions apparently due to neospora caninum in two swiss dairy herds were investigated by means of a pcr and elisa, and other potential causes were eliminated. in addition, a case-control study of 24 case herds and 24 control herds indicated that n caninum-associated abortions were more likely to occur in herds with antibodies to coxiella burnetii (with an odds ratio [or] of 3.38 with a 95 per cent confidence interval [ci] of 1.82 to 6.22), whereas the likelihood was less in herds with antibodi ... | 2002 | 12019533 |
| trigeminal neuropathy in dogs: a retrospective study of 29 cases (1991-2000). | the medical records of 29 dogs unable to close their mouths due to flaccid paralysis or paresis of the muscles innervated by the mandibular branch of the trigeminal nerve, were reviewed. idiopathic trigeminal neuropathy was diagnosed in 26 dogs based on complete resolution of clinical signs and lack of any long-term neurological disease. of these dogs, golden retrievers were overrepresented. no age, sex, or seasonal predispositions were identified. trigeminal sensory innervation deficits were ob ... | 2002 | 12022413 |
| serological survey of neospora caninum infection in dairy cattle herds in aguascalientes, mexico. | a serological survey for antibody activity to neospora caninum was carried out in aguascalientes, a state in the central part of mexico. one-hundred and eighty-seven serum samples from 13 dairy herds were tested by the elisa test. the herd prevalence was 100% and the overall prevalence was 59% (n=110). seventy-six of 97 seropositive cows had previous records of abortion. there was a statistically significant difference between the groups (p<0.05). however, the odds ratio was 1.4, suggesting an a ... | 2002 | 12031815 |
| occurrence of neospora caninum antibodies in sera from dogs of the city of são paulo, brazil. | neospora caninum is an important cause of abortion in dairy cattle worldwide. dogs are important in the epidemiology of this parasite because they are the only hosts known to excrete n. caninum oocysts. in order to understand the prevalence of n. caninum in dogs, sera from 500 owned dogs and from over 600 feral street dogs from the city of são paulo, brazil were assayed for antibodies to n. caninum. sera were examined by the neospora agglutination test (nat) using mouse-derived tachyzoites. anti ... | 2002 | 12031819 |
| hepatic sarcocystosis in a striped dolphin (stenella coeruleoalba) from the spanish mediterranean coast. | fatal hepatic sarcocystosis was diagnosed in a striped dolphin (stenella coeruleoalba) from the northeastern spanish mediterranean coast based on pathologic findings and the microscopic and ultrastructural characteristics of the intralesional parasite. main gross lesions were icterus, subcutaneous hemorrhages, and hepatic congestion. the most prominent microscopic lesions consisted of severe acute multifocal to coalescing necrotizing hepatitis with cholestasis and intralesional protozoa. there w ... | 2002 | 12053971 |
| immune factors influencing the course of infection with neospora caninum in the murine host. | this paper investigates the role of specific immune factors on the course of infection in genetic knockout (gko) mice infected with 3 different strains of neospora caninum. survival time and parasite persistence were examined in interferon-gamma (ifn-gamma), tumor necrosis factor receptor-2 (tnfr2), interleukin 10 (il-10), beta 2 microglobulin (beta2m), and inducible nitric oxide synthase (inos2) gko or wild-type (wt) mice following infection with either pathogenic (nc-1 or nc-2) or attenuated ( ... | 2002 | 12053997 |
| susceptibility of psammomys obesus and meriones tristrami to tachyzoites of neospora caninum. | the susceptibility of psammomys obesus (sand rat) and meriones tristrami (tristram's jird) to neospora caninum was investigated by subcutaneous (s.c.) and intraperitoneal (i.p.) inoculation of 10-fold doses of culture-derived tachyzoites. groups of 5 animals were inoculated with doses of 10-10(7) parasites via each route of inoculation. all but 2 of the sand rats inoculated with doses of 10-10(4) parasites succumbed to the infection by 7-18 days postinfection. all jirds inoculated with 10(7) tac ... | 2002 | 12054004 |
| prevalence of neospora caninum antibodies in dogs from dairy cattle farms in parana, brazil. | serum samples from 134 dogs from 22 cattle dairy farms in the northern region of parana state, brazil, were tested for antibodies to neospora caninum in an indirect fluorescent antibody test. antibodies (> or = 1:50) to n. caninum were found in 29 (21.6%) of the 134 dogs, and seropositive dogs were found on 14 (63.6%) of the 22 dairy cattle farrms. the antibody titers of dogs were 1:50 (3 dogs), 1:100 (7 dogs), 1:200 (7 dogs), 1:400 (6 dogs), and > or = 1:800 (6 dogs). the low prevalence (9%) in ... | 2002 | 12054023 |
| prevalence of antibodies to neospora caninum in white-tailed deer, odocoileus virginianus, from the southeastern united states. | serum samples from 305 white-tailed deer (odocoileus virginianus) from 14 states in the southeastern united states were examined for antibodies to neospora caninum using a direct agglutination test. positive agglutination titers were found in 145 (48%) of the white-tailed deer examined: 21 (7%) had titers of 1:25, 92 (30%) had titers of 1:50, and 32 (10%) had titers of > or = 1:500. these findings that antibodies to n. caninum are common in white-tailed deer support the concept that a sylvatic c ... | 2002 | 12054027 |
| vero cell surface proteoglycan interaction with the microneme protein ncmic(3) mediates adhesion of neospora caninum tachyzoites to host cells unlike that in toxoplasma gondii. | neospora caninum and toxoplasma gondii are characterised by a very low host cell specificity, thus they are able to infect a wide range of different cells in vivo and in vitro. infection of the host cell by tachyzoites is a process which is preceded by adhesion onto the host cell surface. the receptors on the host cell surface which would allow n. caninum to establish a physical interaction have not been investigated so far. here we report the role of host cell surface proteoglycans as receptors ... | 2002 | 12062488 |
| a longitudinal study of neospora caninum infection on a dairy farm in new zealand. | a 600-cow new zealand dairy herd experienced an abortion storm in 1997 and was monitored (blood sampling at about 3-month intervals) from may 1997 until january 1999. abortion risk reached 9% in 1997 and was highest in heifers at 19%. the abortion risk decreased in 1998 to 3.2% (still somewhat higher than during the years prior to the outbreak). the serological reaction pattern for neospora caninum showed an association with abortion risk only around the time of the 1997 outbreak when seropositi ... | 2002 | 12062516 |
| immunisation of mice against neosporosis. | in the present study a murine encephalitis model was used to investigate if protection against neosporosis could be achieved by immunisation. groups of 10 mice were immunised with a sublethal dose of live neospora caninum tachyzoites, n. caninum antigens incorporated into iscoms, n. caninum lysate mixed with quil a, or n. caninum lysate in pbs. control mice were given quil a only. challenge infection with 2.5x10(6) n. caninum tachyzoites resulted in clinical symptoms that remained until the end ... | 2002 | 12062558 |
| pattern of recognition of neospora caninum tachyzoite antigens by naturally infected pregnant cattle and aborted foetuses. | different aspects of neospora tachyzoite antigen recognition by neospora-infected heifers and cows and aborted foetuses were studied. the pattern of antigen recognition and the relationship between ifat titres and number of neospora antigens detected, were evaluated. in addition, the tachyzoite antigens involved in the humoral immune response developed against infection in normal cows and cows that aborted were also characterised throughout pregnancy. comparison of tachyzoite antigen recognition ... | 2002 | 12072210 |
| redescription of neospora caninum and its differentiation from related coccidia. | neospora caninum is a protozoan parasite of animals, which before 1984 was misidentified as toxoplasma gondii. infection by this parasite is a major cause of abortion in cattle and causes paralysis in dogs. since the original description of n. caninum in 1988, considerable progress has been made in the understanding of its life cycle, biology, genetics and diagnosis. in this article, the authors redescribe the parasite, distinguish it from related coccidia, and provide accession numbers to its t ... | 2002 | 12076623 |
| p38-avidity-elisa: examination of herds experiencing epidemic or endemic neospora caninum-associated bovine abortion. | an enzyme-linked immunosorbent assay (elisa) was established to measure the avidity of bovine igg directed against p38, a surface antigen (ncsrs2) of neospora caninum tachyzoites. in the sera of dams intravenously infected with n. caninum nc-1, the p38-specific avidity increased from initially below 40% up to avidity indices between 50 and 80% after days 23 and 91 p.i. the p38-avidity-elisa was used to examine various herds that had experienced endemic and epidemic n. caninum-associated bovine a ... | 2002 | 12079735 |
| serological survey of neospora caninum and leishmania infantum co-infection in dogs. | a seroprevalence survey and risk analysis of neospora caninum and leishmania infantum was conducted in dogs from an area of the campania region of southern italy, in order to investigate the co-infection of these two protozoa. blood samples were collected from 1058 asymptomatic dogs over a 18 months period. serum samples were tested for antibodies to n. caninum and to l. infantum using the indirect fluorescent antibody test. epidemiological data (breed, age, sex, and utilization) were collected ... | 2002 | 12079736 |
| neosporosis in a white rhinoceros (ceratotherium simum) calf. | a 16-day-old white rhinoceros calf died suddenly while in excellent condition and showing no obvious previous clinical signs. it was the 9th calf of a mature female kept free-ranging with 11 other rhinoceros as well as various other game species on a 2000 hectare game breeding centre adjacent to the town of lichtenburg and outlying cattle farmlands in the northern province. at post mortem examination, death was ascribed to heart failure. there was marked multifocal to coalescing subacute parasit ... | 2002 | 12088073 |
| coprodiagnosis of hammondia heydorni in dogs by pcr based amplification of its 1 rrna: differentiation from morphologically indistinguishable oocysts of neospora caninum. | hammondia heydorni is thought to be a non-pathogenic coccidian parasite of dogs that is closely related to neospora caninum, an important parasite of cattle and dogs. oocysts of these two species are morphologically indistinguishable from each other. a population of 2240 dogs in the czech republic was screened for the presence of h. heydorni/n. caninum oocysts and five (0.22%), represented by five of 3135 faecal samples (0.16%), were positive. the internal transcribed spacer 1 region of the rrna ... | 2002 | 12093189 |
| responses of adipose and muscle lipoprotein lipase to chronic infection and subsequent acute lipopolysaccharide challenge. | infection of male swiss webster mice with toxoplasma gondii or neospora caninum leads to long-term alterations in energy balance. following an initial 20 to 30% weight loss in all t. gondii-infected mice, half of the animals regain most of the lost weight (gainers), whereas the others maintain their low body weight (nongainers). infection with n. caninum does not elicit weight loss. lipoprotein lipase (lpl), the enzyme responsible for plasma triglyceride (tg) clearance and partitioning among tis ... | 2002 | 12093671 |
| an analysis of the performance characteristics of serological tests for the diagnosis of neospora caninum infection in cattle. | to analyse the performance characteristics (sensitivity/specificity) of two enzyme-linked immunosorbent assays (elisa) against the indirect fluorescent antibody test (ifat). | 2002 | 12127250 |
| red foxes (vulpes vulpes) are a natural intermediate host of neospora caninum. | the present study was undertaken to determine if red foxes are natural intermediate and/or definitive host for neospora caninum and to study the importance of infection of n. caninum in this species in north-eastern spain. faecal samples and brain tissues were obtained from 122 foxes from 21 rural areas of catalonia. faeces collected were examined for parasite eggs and coccidian oocysts using sucrose flotation. for pcr-based diagnosis of n. caninum in brain tissues, the specific genomic nc5 regi ... | 2002 | 12163240 |
| seroepidemiology of beef and dairy herds and fetal study of neospora caninum in argentina. | the purpose of the present work was to study the epidemiology of neospora caninum in beef and dairy herds in the humid pampas of argentina. the seroprevalence of n. caninum was evaluated in 2414 serum samples of cows from beef and dairy farms. an indirect fluorescent antibody test (ifat) was used to determine specific antibodies. the sera was screened at a dilution >or=1:200 and >or=1:600 in cows with reproductive disease antecedents and without them, respectively. cows without history of reprod ... | 2002 | 12163242 |
| mechanisms of apoptosis in murine fibroblasts by two intracellular protozoan parasites, toxoplasma gondii and neospora caninum. | studies to clarify the mechanisms of apoptosis in host cells, a31 (balb/3t3 clone a31 fibroblasts), caused by two intracellular protozoan parasites, toxoplasma gondii and neospora caninum, were carried out in an in vitro system. the viability of n. caninum-infected cells was significantly reduced following treatment with mouse interferon (ifn)-gamma. by contrast, mouse ifn-gamma treatment had no significant effect on the induction of apoptosis in t. gondii-infected cells. apoptosis of n. caninum ... | 2002 | 12164820 |
| characterization of an outbred pregnant mouse model of neospora caninum infection. | fetal loss and vertical transmission of neospora caninum were evaluated in outbred quackenbush (qs) mice with respect to dose of parasites, n. caninum isolate, and route of injection. mice were infected with nc-liverpool or nc-sweb1 at day 5 or 8 of pregnancy with doses of 10(4), 10(6), or 10(7) parasites, through either a subcutaneous or intraperitoneal injection. polymerase chain reaction was used to detect n. caninum in the brains of offspring, and enzyme-linked immunosorbent assay was used t ... | 2002 | 12197115 |