Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| structural and evolutionary adaptation of rhoptry kinases and pseudokinases, a family of coccidian virulence factors. | the widespread protozoan parasite toxoplasma gondii interferes with host cell functions by exporting the contents of a unique apical organelle, the rhoptry. among the mix of secreted proteins are an expanded, lineage-specific family of protein kinases termed rhoptry kinases (ropks), several of which have been shown to be key virulence factors, including the pseudokinase rop5. the extent and details of the diversification of this protein family are poorly understood. | 2013 | 23742205 |
| sag2a protein from toxoplasma gondii interacts with both innate and adaptive immune compartments of infected hosts. | toxoplasma gondii is an intracellular parasite that causes relevant clinical disease in humans and animals. several studies have been performed in order to understand the interactions between proteins of the parasite and host cells. sag2a is a 22 kda protein that is mainly found in the surface of tachyzoites. in the present work, our aim was to correlate the predicted three-dimensional structure of this protein with the immune system of infected hosts. | 2013 | 23735002 |
| first isolation of hammondia heydorni from dogs in china. | fecal samples of 945 dogs were examined microscopically in 2 refuge facilities in china from march 2010 to november 2011. in 8 dogs, oocysts, 9-14 μm in size, were found. their morphology was similar to those of hammondia heydorni and neospora caninum. sporulated hammondia/neospora-like oocysts were fed to 2 dogs, 2 gerbils, 2 guinea pigs, and 2 km mice; tissues from these inoculated animals were then fed to coccidia-free dogs to determine species susceptibility to these oocysts. oocysts were no ... | 2013 | 23731857 |
| gra 14, a novel dense granule protein from neospora caninum. | 2013 | 23722878 | |
| generation and immunity testing of a recombinant adenovirus expressing ncsrs2-ncgra7 fusion protein of bovine neospora caninum. | neospora caninum is the etiologic agent of bovine neosporosis, which affects the reproductive performance of cattle worldwide. the transmembrane protein, ncsrs2, and dense-granule protein, ncgra7, were identified as protective antigens based on their ability to induce significant protective immune responses in murine neosporosis models. in the current study, ncsrs2 and ncgra7 genes were spliced by overlap-extension pcr in a recombinant adenovirus termed ad5-ncsrs2-ncgra 7, expressing the ncsrs2- ... | 2013 | 23710096 |
| dose-dependent immunogenicity of a soluble neospora caninum tachyzoite-extract vaccine formulated with a soy lecithin/β-glucan adjuvant in cattle. | mice immunized with a soluble extract of neospora caninum tachyzoites (sncag) formulated with providean-avec, an aqueous soy-based adjuvant, are fully protected from n. caninum multiplication. here we evaluated the dose-dependent immunogenicity of this vaccine formulation in cattle. cattle (n=3 per group) were immunized with two applications (30 days apart) of formulations containing providean-avec and different payloads of sncag (100, 50 and 10 μg), that were five to fifty times lower than the ... | 2013 | 23692926 |
| comparison of use of vero cell line and suspension culture of murine macrophage to attenuation of virulence of neospora caninum. | in this study the tachyzoite yields of neospora caninum were compared in two cell lines: vero (african green monkey kidney) and suspension culture of murine macrophage (j774) cell lines. then, n. caninum were continuously passaged in these cell lines for 3 months and the effect of host cells on virulence of tachyzoites was assessed by broiler chicken embryonated eggs. inoculation was performed in the chorioallantoic (ca) liquid of the embryonated eggs with different dilutions (0.5 × 10(4), 1.0 × ... | 2013 | 23684321 |
| expression of neospora caninum ncsrs2 surface protein in pichia pastoris and its application for serodiagnosis of neospora infection. | neospora caninum is considerd a major cause of abortion in cattle worldwide. the antigenic domain of ncsrs2 in n. caninum is an important surface antigen present in the membrane of this parasite. in the present study, the pichia pastoris expression system proved to be a useful tool for the production of recombinant protein. the truncated ncsrs2 gene (by removal of the n-terminal hydrophobic sequence), was cloned in the vector ppiczalphab, and integrated on the genome of the methylotrophic yeast ... | 2013 | 23683365 |
| cloning, nucleotide sequencing and bioinformatics study of ncsrs2 gene, an immunogen from iranian isolate of neospora caninum. | neosporosis is caused by an obligate intracellular parasitic protozoa neospora caninum which infect variety of hosts. ncsrs2 is an immuno-dominant antigen of n. caninum which is considered as one of the most promising targets for a recombinant or dna vaccine against neosporosis. as no study has been carried out to identify the molecular structure of n. caninum in iran, as first step, we prepared a scheme to identify this gene in this parasite in iran. | 2013 | 23682269 |
| evaluation of cross immunity and histopathological findings in experimentally infected balb/c mice with neospora caninum and besnoitia caprae. | caprine besnoitiosis is an economically important disease of goats. neospora caninum, another coccidian parasite of worldwide distribution, infects several animal species and is a major cause of abortion in cattle. combined infections of n. caninum and besnoitia caprae can occur in geographical areas endemic for both species of parasite in goats. this experiment was conducted to investigate the possible cross-immunity between these two infections in experimentally infected balb/c mice. | 2013 | 23682267 |
| neospora caninum nc-6 argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams. | neospora caninum infection is a major cause of abortion in cattle. the objectives of this study were to genetically characterize the n. caninum nc-6 argentina isolate using a multilocus microsatellite analysis approach and to study its biological behavior by experimental inoculations into seronegative and seropositive pregnant cattle, evaluating the humoral and cellular immune response elicited and the occurrence of transplacental transmission and fetopathy. pregnant cows (65 days of gestation) ... | 2013 | 23666227 |
| parasiticidal activity of a novel synthetic peptide from the core α-helical region of nk-lysin. | nk-lysin is an anti-microbial peptide that plays a critical role in innate immunity against infectious pathogens through its selective membrane disruptive property. we previously expressed and purified a full-length chicken nk-lysin (cnkl) recombinant protein, and demonstrated its in vitro anti-parasitic activity against the apicomplexan protozoan, eimeria, the etiologic agent of avian coccidiosis. this study evaluated the in vitro and in vivo anti-parasitic properties of a synthetic peptide (cn ... | 2013 | 23664157 |
| bovine neosporosis: clinical and practical aspects. | neospora caninum is a protozoan parasite with a wide host range but with a preference for cattle and dogs. since the description of n. caninum as a new genus and species in 1988, bovine neosporosis has become a disease of international concern as it is among the main causes of abortion in cattle. at present there is no effective treatment or vaccine. this review focuses on the epidemiology of the disease and on prospects for its control in cattle. finally, based on the implications of clinical f ... | 2013 | 23659742 |
| neospora caninum: infection induces high lysosomal activity. | neospora caninum is a protozoan that causes abortion in cattle and neuromuscular lesions in dogs, making it an important target of veterinary medicine. lysosomes are cellular organelles responsible for important biological functions as cellular defense mechanisms. the aim of this work was to evaluate the lysosomal stability of rat gliocytes infected in vitro with n. caninum. rat glial cultures were infected at a ratio of 1:1 (cell/parasite). the enzymatic activity of acid phosphatase (orthophosp ... | 2013 | 23648665 |
| a novel dense granule protein, gra22, is involved in regulating parasite egress in toxoplasma gondii. | the intracellular protozoan parasite toxoplasma gondii is capable of invading any nucleated cell and replicates within a parasitophorous vacuole (pv). this microenvironment is modified by secretory proteins from organelles named rhoptries and dense granules. in this report, we identify a novel dense granule protein, which we refer to as gra22. gra22 has no significant homology to any other known proteins. gra22 possesses a signal peptide at the n-terminal end which is responsible for dense granu ... | 2013 | 23623919 |
| fecal shedding of toxocara canis and other parasites in foxes and coyotes on prince edward island, canada. | knowledge of parasites shed by wild canids can assist in recognizing risk to human and domestic animal health. our aim was to estimate the prevalence of patent infections with toxocara canis and other parasites in foxes (vulpes vulpes) and coyotes (canis latrans) in prince edward island, canada. identification of parasite species was based on microscopic examination of feces, with the use of a sucrose fecal flotation method. sample collection was performed in winter on carcasses of 271 and 185 h ... | 2013 | 23568915 |
| congenital transmission of neospora caninum in white-tailed deer (odocoileus virginianus). | neosporosis is an important cause of bovine abortion worldwide. many aspects of transmission of neospora caninum in nature are unknown. the white-tailed deer (odocoileus virginianus) is considered one of the most important wildlife reservoirs of n. caninum in the usa. during the hunting seasons of 2008, 2009, and 2010, brains of 155 white-tailed deer fetuses were bioassayed in mice for protozoal isolation. viable n. caninum (ncwtdmn1, ncwtdmn2) was isolated from the brains of two fetuses by bioa ... | 2013 | 23566408 |
| diagnosis and isolation of toxoplasma gondii in horses from brazilian slaughterhouses. | this study aimed to investigate anti-toxoplasma gondii antibodies and to isolate the parasite from the brains of horses processed at slaughterhouses in brazil. we collected brain and blood samples from 398 horses of various ages, from six brazilian states. serum samples were evaluated by indirect fluorescent antibody test (ifat cut-off titre ≥ 1:64), and brains were submitted to mouse bioassay. among the 398 horses, positivity for t. gondii was identified in 46 (11.6%) by ifat and in 14 (3.5%) b ... | 2013 | 23538498 |
| antibody kinetics in goats and conceptuses naturally infected with neospora caninum. | neospora caninum is a protozoan which can cause abortions in caprines. however, information regarding the humoral immune response and the occurrence of reproductive disorders is scarce. this is the first study in which the kinetics of antibodies is studied in pregnant goats naturally infected by n. caninum, as well as their respective conceptuses. the subclasses of igg (igg1 and igg2) were also evaluated in pregnant goats. reproductive problems related to neosporosis (abortion and stillbirth) oc ... | 2013 | 23537945 |
| efficacy of decoquinate against sarcocystis neurona in cell cultures. | decoquinate is a quinolone anticoccidial approved for use in the prevention of intestinal coccidiosis in farm animals. this compound has good activity against toxoplasma gondii and neospora caninum in cell cultures. the drug acts on the parasites' mitochondria. the activity of decoquinate against developing merozoites of 2 isolates of sarcocystis neurona was examined in cell culture. merozoite production at 10 days was completely inhibited when decoquinate was used at 20 or 240 nm. the ic50 of d ... | 2013 | 23523012 |
| [seroprevalence of neospora caninum in dairy cattle farms in algeria's central northern region]. | as there has been little research into the epidemiological status of bovine neosporosis in algeria, the main aims of this study were to confirm the circulation of neospora caninum in the country and to determine its seroprevalence in dairy cattle in five provinces of algeria's central northern region by means of enzyme-linked immunosorbent assay and indirect immunofluorescence. a serological study of 186 dairy cattle declared brucellosis-free established an overall seroprevalence of 12.37%, conf ... | 2012 | 23520748 |
| display of neospora caninum surface protein related sequence 2 on rous sarcoma virus-derived gag protein virus-like particles. | virus-like particles (vlps) displaying antigen have been increasingly recognized as a potential vaccine in the livestock industry. in this study, neospora caninum surface protein related sequence (ncsrs)2 was displayed on the surface of rous sarcoma virus group-antigen protein (rsv-gag) vlps. two types of bombyx mori nucleopolyhedrovirus (bmnpv) bacmids, encoding rsv-gag and ncsrs2 genes, were co-injected into silkworm larvae to produce vlps-ncsrs2. at 7 days post-injection, vlps-ncsrs2 were col ... | 2013 | 23511652 |
| serologic survey of infectious diseases in populations of maned wolf (chrysocyon brachyurus) and crab-eating fox (cerdocyon thous) from aguas emendadas ecological station, brazil. | domestic dogs are reservoirs for many infectious diseases and may represent a potential source of infection for wild canid populations. a serologic investigation of antibodies to toxoplasma gondii, neospora caninum, brucella abortus, and leptospira spp. was conducted on three maned wolves (chrysocyon brachyurus) and seven crab-eating foxes (cerdocyon thous), all free-living, at the aguas emendadas ecological station (esecae), federal district, brazil, between february and october 2006. out of th ... | 2013 | 23505716 |
| mice congenitally infected with low-to-moderate virulence neospora caninum isolates exhibited clinical reactivation during the mating period without transmission to the next generation. | endogenous transplacental transmission (entt) is the major transmission route of neospora caninum in cattle. thus, the development of a standardised experimental model of entt is needed for more appropriate testing of parasite biology and control strategies. a recent study reported up to 40-50% entt rates in chronically infected dams with either high or low-to-moderate virulence isolates, although low fertility rates were observed in dams inoculated with the high virulence isolate. therefore, lo ... | 2013 | 23501806 |
| first report of seroprevalence of toxoplasma gondii and neospora caninum in dairy sheep from humid pampa, argentina. | the aim of this study was to describe the occurrence of antibodies to toxoplasma gondii and neospora caninum in dairy sheep from the humid pampa region, argentina. blood samples from 704 dairy sheep belonging to six flocks were collected. using a cut off titer of 1:50, an indirect fluorescence antibody test was used. antibodies to t. gondii or n. caninum were detected in 17.3 % (n = 122) and 3 % (n = 21), respectively. all the flocks had at least one seropositive animal to t. gondii but two of t ... | 2013 | 23494577 |
| development of a disperse dye immunoassay technique for detection of antibodies against neospora caninum in cattle. | in this study a disperse dye immunoassay method was standardized and evaluated for detection of antibodies against neospora caninum in cattle. sera from 150 cattle with a recent history of abortion were collected and tested by commercial elisa kit and a standardized in-house dye immunoassay system. the positivity rate for the sera used in this study was 34.6% for the disperse dye immunoassay (ddia) compared to 32% obtained by elisa kit. this study showed no significant difference between ddia an ... | 2013 | 23467930 |
| neospora caninum surface antigen (p40) is a potential diagnostic marker for cattle neosporosis. | neospora caninum is an intracellular protozoan that infects domestic and wild canids as well as many warm-blooded animals as shown by the isolation of viable parasites. the effectiveness of diagnostic tests for detecting specific antibodies against n. caninum is hampered by potential cross-reaction with other coccidia. so, there is currently an urgent need for a sensitive and specific diagnostic assay for detecting n. caninum in animals. the n. caninum 40-kd surface antigen (p40), similar to ncs ... | 2013 | 23435920 |
| toxoplasmosis and neosporosis among beef cattle slaughtered for food in western thailand. | beef is a main type of meat consumed by thais. the prevalences of anti-toxoplasma gondii and anti-neospora caninum antibodies were investigated among beef cattle slaughtered for food in western thailand. a total of 389 blood samples obtained from beef cattle from 24 herds were collected at 3 slaughterhouses in 3 western provinces of thailand: kanchanaburi, ratchaburi and nakhon pathom. an indirect immunofluorescent antibody test (ifat) was performed using cut-off values of 1:128 for t. gondii an ... | 2012 | 23431814 |
| risk factors associated with seroprevalence of neospora caninum in dogs from urban and rural areas of milk and coffee production in minas gerais state, brazil. | this study was conducted to determine the seroprevalence of anti-neospora caninum antibodies and to investigate the risk factors related to seroprevalence in dogs from urban and rural areas with distinct economic activities (milk and coffee production) in minas gerais state, brazil. for this purpose, blood samples from 703 dogs were collected and questionnaires addressing epidemiological aspects were completed by dog-owners. the sera were analysed for anti-n. caninum antibodies by indirect fluor ... | 2013 | 23419686 |
| development of an immunochromatographic assay based on dense granule protein 7 for serological detection of toxoplasma gondii infection. | dense granule antigen proteins derived from toxoplasma gondii (tggras) are potential antigens for the development of diagnostic tools. tggra7 and tggra14 were detected in the peritoneal fluid of t. gondii-infected mice, suggesting that tggras may be highly antigenic proteins. here, tggra7 and tggra14 were evaluated as candidates for the development of a marker for a rapid diagnostic test. the specificity and sensitivity of purified recombinant proteins of tggra7 and tggra14 were compared in an i ... | 2013 | 23408523 |
| fatal toxoplasmosis in a vinaceous amazon parrot (amazona vinacea). | toxoplasmosis was diagnosed in a vinaceous amazon parrot based on histopathology and immunohistochemistry. the bird was prostrate on the bottom of the cage and died. necropsy revealed edema and congestion of the lungs, cloudy air sacs, and mild hepatomegaly. histopathology revealed severe pulmonary congestion and edema and interstitial mononuclear cell inflammation associated with many cysts containing bradyzoites of toxoplasma gondii scattered throughout. the heart had mild multifocal lymphocyt ... | 2012 | 23397856 |
| characterisation of full-length mitochondrial copies and partial nuclear copies (numts) of the cytochrome b and cytochrome c oxidase subunit i genes of toxoplasma gondii, neospora caninum, hammondia heydorni and hammondia triffittae (apicomplexa: sarcocystidae). | genomic dna was extracted from three oocyst isolates of hammondia triffittae from foxes and two oocyst isolates of hammondia heydorni from dogs, as well as from cell culture-derived tachyzoites of toxoplasma gondii (rh strain) and neospora caninum (nc-liverpool strain), and examined by pcr with primers targeting the cytochrome b (cytb) and the cytochrome c oxidase subunit i (cox1) genes in order to characterise both genes and, if possible, the remainder of the mitochondrial genome of these speci ... | 2013 | 23358734 |
| effects of neospora caninum infection on brain microvascular endothelial cells bioenergetics. | the brain is the most commonly affected organ during neospora caninum infection but the mechanisms utilized by this protozoan parasite for traversal of the blood-brain barrier (bbb) are not yet understood. herein, we investigate the cellular pathogenicity of n. caninum infection on bioenergetics of human brain microvascular endothelial cells (hbmecs), a fundamental component of the bbb. | 2013 | 23351811 |
| specific antibody responses against neospora caninum recombinant rncgra7, rncsag4, rncbsr4 and rncsrs9 proteins are correlated with virulence in mice. | the intraspecific diversity of neospora caninum is a determinant for in vivo parasite virulence and in vitro parasite behaviour. the relationship between isolate virulence and specific antibody responses against key parasite proteins has not been well characterized. the response kinetics and the differences in specific anti-rncgra7, -rncsag4, -rncbsr4 and -rncsrs9 antibody levels were analysed by recombinant protein-based elisa in groups of mice inoculated with 10 different n. caninum isolates t ... | 2013 | 23343779 |
| neospora caninum causes severe economic losses in cattle in the humid pampa region of argentina. | this work estimates the economic losses due to neospora abortions in the humid pampa region of argentina.the total dairy and beef cattle population at risk of abortion is 1,771,326 and 9,726,684 head, respectively. in dairy cattle, there was an 8 % risk of experiencing abortion due to a variety of causes, but 16.5 % of them were due to neospora caninum. the economic losses were estimated at us$1,415 (1,400-1,431) per abortion, which equates to a total loss of us$33,097,221 (15,622,600-119,349,69 ... | 2013 | 23325013 |
| development of two murine antibodies against neospora caninum using phage display technology and application on the detection of n. caninum. | neosporosis, caused by an intracellular parasite, neospora caninum, is an infectious disease primarily of cattle and dogs. it occurs worldwide and causes huge damages to dairy farms. in this study, we immunized mice with recombinant surface-associated protein 1 of n. caninum (rncsag1) and developed two novel monoclonal antibodies, a10 and h3, against ncsag1 using phage-display technology. both clones bound to purified rncsag1 and the half maximal inhibitory concentrations of a10 and h3 are 50 an ... | 2013 | 23308179 |
| proteins mediating the neospora caninum-host cell interaction as targets for vaccination. | neospora caninum is an apicomplexan parasite that is capable of infecting, a wide range of tissues. the fact that neospora represents an important abortion-causing parasite in cattle has transformed neosporosis research from an earlier, rather esoteric field, to a significant research topic, and considerable investments have been made in the last years to develop an efficacious vaccine or other means of intervention that would prevent infection and abortion due to n. caninum infection in cattle. ... | 2013 | 23276967 |
| antimicrobial effects of murine mesenchymal stromal cells directed against toxoplasma gondii and neospora caninum: role of immunity-related gtpases (irgs) and guanylate-binding proteins (gbps). | mesenchymal stromal cells (mscs) have a multilineage differentiation potential and provide immunosuppressive and antimicrobial functions. murine as well as human mscs restrict the proliferation of t cells. however, species-specific differences in the underlying molecular mechanisms have been described. here, we analyzed the antiparasitic effector mechanisms active in murine mscs. murine mscs, in contrast to human mscs, could not restrict the growth of a highly virulent strain of toxoplasma gondi ... | 2012 | 23269418 |
| the rhoptry proteome of eimeria tenella sporozoites. | proteins derived from the rhoptry secretory organelles are crucial for the invasion and survival of apicomplexan parasites within host cells. the rhoptries are club-shaped organelles that contain two distinct subpopulations of proteins that localise to separate compartments of the organelle. proteins from the neck region (rhoptry neck proteins, ron) are secreted early in invasion and a subset of these is critical for the formation and function of the moving junction between parasite and host mem ... | 2012 | 23262303 |
| effects of ifn-γ, tnf-α, il-10 and tgf-β on neospora caninum infection in rat glial cells. | neospora caninum causes abortion in cattle and neurological disorders in dogs. the immunological response to this parasite has been described as predominantly of the th1 type. however, infected primary glial cell cultures release il-10 and il-6 but not ifn-γ. this suggests a rather protective response of the glia to avoid inflammatory damage of the nervous tissue. in this study, we investigated the effects of pro-inflammatory cytokines in primary mixed cultures of rat astrocytes and microglia in ... | 2012 | 23262170 |
| seroprevalence of neospora caninum and associated risk factors in intensive or semi-intensively managed dairy and breeding cattle of ethiopia. | a cross-sectional study of neospora caninum infection was conducted in major milksheds of ethiopia. cattle (n=2334) from 273 farms were bled and the sera screened for antibodies against n. caninum using a commercial elisa kit. herd and individual animal level data were collected from farm records and a semi-structured questionnaire format. the overall animal level seroprevalence was 13.3%, while the prevalence at farm level was 39.6%. in urban and peri-urban smallholder dairy farms, the seroprev ... | 2013 | 23261087 |
| what is the global economic impact of neospora caninum in cattle - the billion dollar question. | neospora caninum is regarded as one of the most important infectious causes of abortions in cattle worldwide, yet the global economic impact of the infection has not been established. a systematic review of the economic impact of n. caninum infections/abortions was conducted, searching pubmed with the terms 'cattle' and 'neospora'. this yielded 769 publications and the abstracts were screened for economically relevant information (e.g. abortion prevalence and risk, serological prevalence). furth ... | 2013 | 23246675 |
| gene cloning and characterization of the protein encoded by the neospora caninum bradyzoite-specific antigen gene bag1. | neospora caninum is an apicomplexan parasite that causes repeated abortion and stillbirth in cattle. the aim of this study was to clone the gene encoding the n. caninum orthologue (ncbag1) of the toxoplasma gondii bradyzoite-specific protein tgbag1 and characterize its expression pattern in the parasite. isolation of the full-length 684-bp gene revealed that it shared 78.3% sequence similarity with tgbag1. ncbag1 encodes a predicted protein of 227 amino acids with 80.3% similarity to tgbag1. a p ... | 2013 | 23245337 |
| tissue distribution of neospora caninum in experimentally infected cattle. | histopathology and quantitative pcr (qpcr) were used to determine the tissue distribution of neospora caninum in calves at 80 days postinfection. our findings revealed that the most appropriate brain areas for researching n. caninum pathogenesis were the amygdala and hippocampus for qpcr and the corpus striatum and diencephalon for histopathology. | 2013 | 23239805 |
| comparative study of protective activities of neospora caninum bradyzoite antigens, ncbag1, ncbsr4, ncmag1, and ncsag4, in a mouse model of acute parasitic infection. | neospora caninum is an obligate intracellular protozoan parasite that causes severe neuromuscular diseases, repeated abortion, stillbirth, and congenital infection in livestock and companion animals. the development of an effective vaccine against neosporosis in cattle is an important issue due to the significant worldwide economic impact of this disease. we evaluated the immunogenicity of four bradyzoite antigens, ncbag1 (first described in this study), ncbsr4, ncmag1, and ncsag4, using an acut ... | 2013 | 23229317 |
| high rate of transplacental infection and transmission of neospora caninum following experimental challenge of cattle at day 210 of gestation. | in order to investigate the pathogenesis of neosporosis following a primary infection in late pregnancy, cattle were subcutaneously challenged with 5 × 108neospora caninum (nc1 isolate) tachyzoites at day 210 of gestation and serial necropsies were then carried out at 14, 28, 42 and 56 days post-infection (dpi). no abortions occurred and all the foetuses were viable at the time of euthanasia. there was a high rate of vertical transmission, as parasites were detected by immunohistochemical labell ... | 2012 | 23228067 |
| plasma concentrations of pregnancy-associated glycoproteins measured using anti-bovine pag-2 antibodies on day 120 of gestation predict abortion in dairy cows naturally infected with neospora caninum. | the present study sought to determine: (i) the effects of neospora caninum infection and twin pregnancy on plasma pregnancy-associated glycoprotein-2 (pag-2) concentrations throughout pregnancy and (ii) whether plasma pag-2 concentrations could predict abortion in n. caninum-infected cows. the study was performed on a commercial holstein-friesian dairy herd in northeastern spain and the final data included those recorded in 53 non-aborting and 19 aborting animals. blood samples were collected im ... | 2013 | 23228018 |
| factors associated with seroprevalence of neospora caninum in dairy cattle in southeastern brazil. | neosporosis is an infectious disease caused by neospora caninum, an obligate intracellular cyst-forming protozoan considered a major cause of miscarriage in dairy cattle in many parts of the world. this cross-sectional study evaluated the relationship between reproductive abnormalities and seropositivity to n. caninum in 1,204 dairy cows from 40 farms located in the state of minas gerais, southeastern brazil. producers were interviewed, and blood samples were collected to perform indirect immuno ... | 2013 | 23212838 |
| seroprevalence for neospora caninum in goats of minas gerais state, brazil. | this study was aimed at determining the prevalence of anti-neospora caninum antibodies in serum samples from 667 goats, collected from 90 herds in 90 municipalities in eight of the twelve mesoregions of minas gerais state. the presence of anti-n. caninum antibodies was determined by ifat with a cut-off value of 1:50. the true herd-level seroprevalence in the entire study area was 75.2% (ci 95%: 59.5-90.9%). the true individual-level seroprevalence in the entire study area was 10.7% (ci 95%: 8.3- ... | 2013 | 23200513 |
| library of apicomplexan metabolic pathways: a manually curated database for metabolic pathways of apicomplexan parasites. | the library of apicomplexan metabolic pathways (lamp, http://www.llamp.net) is a web database that provides near complete mapping from genes to the central metabolic functions for some of the prominent intracellular parasites of the phylum apicomplexa. this phylum includes the causative agents of malaria, toxoplasmosis and theileriosis-diseases with a huge economic and social impact. a number of apicomplexan genomes have been sequenced, but the accurate annotation of gene function remains challe ... | 2013 | 23193253 |
| on the efficacy and safety of vaccination with live tachyzoites of neospora caninum for prevention of neospora-associated fetal loss in cattle. | infection of cattle with neospora caninum may result in abortion or the birth of a congenitally infected calf. vaccination with live n. caninum protects against experimental infection of cattle and mice, and the naturally attenuated nc-nowra strain of n. caninum is of particular interest as a potential vaccine candidate. vaccination of heifers prior to breeding with live nc-nowra tachyzoites by either the subcutaneous or the intravenous route reduced the rate of abortion and the presence of the ... | 2013 | 23175289 |
| babesia divergens and neospora caninum apical membrane antigen 1 structures reveal selectivity and plasticity in apicomplexan parasite host cell invasion. | host cell invasion by the obligate intracellular apicomplexan parasites, including plasmodium (malaria) and toxoplasma (toxoplasmosis), requires a step-wise mechanism unique among known host-pathogen interactions. a key step is the formation of the moving junction (mj) complex, a circumferential constriction between the apical tip of the parasite and the host cell membrane that traverses in a posterior direction to enclose the parasite in a protective vacuole essential for intracellular survival ... | 2013 | 23169033 |
| novel tools for the diagnosis and differentiation of acute and chronic bovine besnoitiosis. | diagnosis of acute bovine besnoitiosis is a major diagnostic problem. we developed diagnostic tests to serologically diagnose and differentiate acute and chronic cases of bovine besnoitiosis using affinity purified antigens of besnoitia besnoiti tachyzoites in immunoblots and in both, a conventional elisa and an avidity elisa. sera of acutely and chronically infected cattle were investigated using these tests. acutely infected cattle initially recognised an antigen of 74 kda relative molecular m ... | 2013 | 23160022 |
| temporal trends in bulk milk antibodies to salmonella, neospora caninum, and leptospira interrogans serovar hardjo in irish dairy herds. | bulk milk samples were collected from 309 irish dairy herds at four time points during 2009 and tested for antibodies to salmonella spp., n. caninum, and l. hardjo, three abortifacient agents in irish dairy herds. of the 312 study herds, 49% vaccinated against salmonella and 76% vaccinated against l. hardjo. in unvaccinated herds, the overall prevalence of antibody positive herds was 49% for salmonella, 19% for n. caninum and 86% for l. hardjo. there was no association between both testing posit ... | 2013 | 23117041 |
| genetic mapping and coccidial parasites: past achievements and future prospects. | coccidial parasites including cryptosporidium parvum, cyclospora cayetanensis, neospora caninum, toxoplasma gondii and the eimeria species can cause severe disease of medical and veterinary importance. as many as one-third of the human population may carry t. gondii infection, and eimeria are thought to cost the global poultry production industry in excess of us$2 billion per annum. despite their significance, effective vaccines are scarce and have been confined to the veterinary field. as seque ... | 2012 | 23107923 |
| adjuvant and immunostimulatory effects of a d-galactose-binding lectin from synadenium carinatum latex (scll) in the mouse model of vaccination against neosporosis. | vaccination is an important control measure for neosporosis that is caused by a coccidian parasite, neospora caninum, leading to abortion and reproductive disorders in cattle and serious economic impacts worldwide. a d-galactose-binding lectin from synadenium carinatum latex (scll) was recently described by our group with potential immunostimulatory and adjuvant effects in the leishmaniasis model. in this study, we evaluated the adjuvant effect of scll in immunization of mice against neosporosis ... | 2012 | 23107170 |
| expression, purification and antigenicity of neospora caninum-antigens using silkworm larvae targeting for subunit vaccines. | infection of neospora caninum causes abortion in cattle, which has a serious worldwide impact on the economic performance of the dairy and beef industries. now, inexpensive and efficacious vaccines are required to protect cattle from neosporosis in livestock industry. in this study, n. caninum surface antigen 1 (sag1) and sag1-related sequence 2 (srs2) were expressed in hemolymph of silkworm larvae as a soluble form. expressed sag1 and srs2 clearly showed antigenicity against n. caninum-positive ... | 2013 | 23102762 |
| detection of neospora caninum in wild carnivorans in great britain. | samples of brain and other tissues were collected from 99 ferrets (mustela furo), 83 red foxes (vulpes vulpes), 70 european polecats (mustela putorius), 65 american mink (neovison vison), 64 eurasian badgers (meles meles) and 9 stoats (mustela erminea), from around great britain. dna was extracted from approximately 1g of tissue and tested by specific nested its1 pcr for neospora caninum. the results from the pcr demonstrated that neospora specific dna was detected in all species of wild carnivo ... | 2013 | 23102760 |
| [advances in the development of vaccines for bovine neosporosis]. | neosporosis, a disease caused by the obligate intracellular protozoan neospora caninum, produces abortions in cattle. the severe economic losses in cattle industry justify the need to develop control measures for preventing bovine abortion. apicomplexan parasitic resistance is associated with t helper 1 immune response mediated by cd4 cytotoxic t lymphocytes, the production of interferon-gamma, interleukin-12, tumor necrosis factor and immunoglobulin g2. the reduction of vertical transmission in ... | 2012 | 23102471 |
| quantification of vertical transmission of neospora caninum in dairy cows in minas gerais, brazil. | the objective of this cross-sectional study was to estimate the rate of vertical transmission and to investigate horizontal transmission of neospora caninum and occurrences of reproductive abnormalities in seropositive dairy cows on two farms in the state of minas gerais, brazil. the frequency of cows seropositive for n.caninum according to the indirect fluorescent antibody test (ifat) was 39.4% (93/236) for farm a and 31.4% (32/102) for the farm b (p > 0.05). the mean vertical transmission rate ... | 2012 | 23070443 |
| seroprevalence of antibodies to neospora caninum in dogs in the state of alagoas, brazil. | this study investigated occurrences of antibodies to anti-neospora caninum in dogs in the state of alagoas, brazil, and the risk factors associated with the infection. to investigate antibodies against n. caninum, an indirect immunofluorescence antibody test (ifat) was used. to identify risk factors associated with infection by n. caninum, univariate analyses on variables of interest were conducted. the study analyzed 237 samples from dogs of both sexes and different breeds and ages, both from r ... | 2012 | 23070441 |
| experimental infection with neospora caninum in pregnant bitches. | in this study, transplacental transmission of neospora caninum in bitches at different stages of pregnancy was evaluated. three bitches were inoculated in the 3rd week and three in the 6th week of gestation with 10(8) tachyzoites of n. caninum (nc-1 strain). all the infected bitches and at least one of their offspring presented anti-n. caninum antibodies according to the indirect fluorescent antibody test (ifat > 400). the pups and their mothers were sacrificed and tissues from the central nervo ... | 2012 | 23070432 |
| investigating the role of wild carnivores in the epidemiology of bovine neosporosis. | neospora caninum is a protozoan parasite, primarily associated with bovine abortion. the only definitive hosts discovered to date are carnivores. this study aimed to identify the role of mammalian carnivores in the epidemiology of bovine neosporosis. a sample bank of serum, fecal and brain samples was established: american mink (mustela vison), red foxes (vulpes vulpes), pine martens (martes martes), badgers (meles meles), stoats (mustela erminea), otters (lutra lutra) and feral ferrets (mustela ... | 2013 | 23068142 |
| pathogens of wild maned wolves (chrysocyon brachyurus) in brazil. | the maned wolf, chrysocyon brachyurus, is an endangered neotropical canid that survives at low population densities. diseases are a potential threat for its conservation but to date have been poorly studied. we performed clinical evaluations and investigated the presence of infectious diseases through serology and coprologic tests on maned wolves from galheiro natural private reserve, perdizes city, minas gerais state, southeastern brazil. fifteen wolves were captured between 2003 and 2008. we f ... | 2012 | 23060508 |
| low rates of neospora caninum infection reactivation during gestation are observed in both chronically and congenitally infected mice. | endogenous transplacental transmission (entt) of neospora caninum is the most common route of infection in cattle and occurs as a consequence of a reactivation of n. caninum infection that may lead to abortion or to the birth of congenitally infected calves. the reactivation of n. caninum infection was studied during the gestation of chronically infected dams and, for the first time, in their congenitally infected pups. balb/c mice were infected with nc-spain 7 (group 1) or nc-spain 3h (group 2) ... | 2013 | 23058003 |
| differential effects of intranasal vaccination with recombinant ncpdi in different mouse models of neospora caninum infection. | in this study, mice were vaccinated intranasally with recombinant n. caninum protein disulphide isomerase (ncpdi) emulsified in cholera toxin (ct) or cholera toxin subunit b (ctb) from vibrio cholerae. the effects of vaccination were assessed in the murine nonpregnant model and the foetal infection model, respectively. in the nonpregnant mice, previous results were confirmed, in that intranasal vaccination with recncpdi in ct was highly protective, and low cerebral parasite loads were noted upon ... | 2013 | 23046358 |
| reproductive disorders in relation to neospora caninum, brucella spp. and bovine viral diarrhoea virus serostatus in breeding and dairy farms of central and southern ethiopia. | abortion and stillbirth are important reproductive disorders in the dairy industry and are often caused by infectious agents. this study investigated whether bovine viral diarrhoea virus (bvdv), brucella spp., and neospora caninum are associated with abortion and/or stillbirth in dairy cattle in ethiopia. dairy cattle from 99 farms were categorized as cases (n=134) or controls (n=268) according to reproductive data. blood samples were screened for antibodies for these infectious agents. the over ... | 2013 | 23034138 |
| the core mouse response to infection by neospora caninum defined by gene set enrichment analyses. | in this study, the balb/c and qs mouse responses to infection by the parasite neospora caninum were investigated in order to identify host response mechanisms. investigation was done using gene set (enrichment) analyses of microarray data. gsea, manova, romer, subgse and sam-gs were used to study the contrasts neospora strain type, mouse type (balb/c and qs) and time post infection (6 hours post infection and 10 days post infection). the analyses show that the major signal in the core mouse resp ... | 2012 | 23012496 |
| frequency of infection by neospora caninum in wild rodents associated with dairy farms in aguascalientes, mexico. | the purpose of this study was to detect the presence of neospora caninum in tissues from wild rodents associated with dairy farms in aguascalientes, mexico. individuals from mus musculus, rattus novergicus and spermophilus variegatus were caught in dairy farms with history of positive status to n. caninum. a total of 33 rodents were caught from which samples were taken of brain, spinal cord, liver and heart and were tested with nested pcr and immunohistochemistry (ihc). results obtained with pcr ... | 2013 | 22989953 |
| a review of the infection, genetics, and evolution of neospora caninum: from the past to the present. | this paper is a review of current knowledge on neospora caninum in the context of other apicomplexan parasites and with an emphasis on: life cycle, disease, epidemiology, immunity, control and treatment, evolution, genomes, and biological databases and web resources. n. caninum is an obligate, intracellular, coccidian, protozoan parasite of the phylum apicomplexa. infection can cause the clinical disease neosporosis, which most notably is associated with abortion in cattle. these abortions are a ... | 2013 | 22985682 |
| the molecular basis for the distinct host and tissue tropisms of coccidian parasites. | the phylum apicomplexa is home to a variety of parasites of significant medical and economic relevance, including the coccidian species toxoplasma gondii, neospora caninum and eimeria tenella. in spite of their shared ancestry, the aforementioned coccidian species exhibit highly variable host and tissue tropisms; whilst t. gondii invades a broad spectrum of cell types and host organisms, e. tenella infection is restricted to the caecum in chicken. apicomplexans are obligatory intracellular paras ... | 2012 | 22982139 |
| seroprevalence of neospora caninum, paratuberculosis and q fever in cattle in belgium in 2009-2010. | 2012 | 22952130 | |
| granulomatous meningoencephalitis due to toxoplasma gondii in a black-headed night monkey (aotus nigriceps). | this report describes toxoplasmosis infection in a black-headed night monkey (aotus nigriceps) from the zoological park of the federal university of mato grosso, brazil. the animal was submitted alive to the veterinary hospital of the federal university of mato grosso presenting neurologic clinical signs. there were no improvements on the animal's clinical condition after treatment, so it was euthanized. at necropsy, the most significant gross lesions observed were hemorrhages diffusely scattere ... | 2011 | 22946380 |
| vaccination with the recombinant chimeric antigen recncmic3-1-r induces a non-protective th2-type immune response in the pregnant mouse model for n. caninum infection. | the major route of transmission of neospora caninum in cattle is transplacentally from an infected cow to its progeny. therefore, a vaccine should be able to prevent both the horizontal transmission from contaminated food or water and the vertical transmission. we have previously shown that a chimeric vaccine composed of predicted immunogenic epitopes of ncmic3, ncmic1 and ncrop2 (recncmic3-1-r) significantly reduced the cerebral infection in balb/c mice. in this study, mice were first vaccinate ... | 2012 | 22940381 |
| neospora caninum infection in dogs from southern romania: coproparasitological study and serological follow-up. | neospora caninum is an important cause of bovine abortion worldwide for which dogs are the definitive host. the present study was aimed at investigating the exposure to n. caninum infection based on lifestyle categories of dogs from southern romania. for this purpose, randomly selected rural and urban dogs were examined for fecal n. caninum -like oocysts and were serologically tested for the presence of anti- n. caninum igg antibodies. of the 386 dog fecal samples, n. caninum -like oocysts were ... | 2013 | 22931541 |
| effects of age and breed on the prevalence of neospora caninum in commercial dairy cattle from pakistan. | neospora caninum is a major cause of bovine abortion worldwide. a serological survey was carried out to determine the seroprevalence of exposure to n. caninum in dairy cattle based on age and breed from punjab and sindh provinces, pakistan. serum samples from 641 animals from 12 herds from punjab (n = 7) and sindh (n = 5) provinces were tested for antibodies against n. caninum using a commercially available competitive enzyme-linked immunosorbent assay. positive reactions to n. caninum were seen ... | 2013 | 22924907 |
| evaluation of the protection conferred by a naturally attenuated neospora caninum isolate against congenital and cerebral neosporosis in mice. | the parasite neospora caninum is an important abortifacient agent in cattle worldwide. at present, the development of an effective and safe vaccine against bovine neosporosis is of great relevance. recently, a new isolate of n. caninum (nc-spain 1 h) which was obtained from the brain of an asymptomatic congenitally infected calf, exhibited non-virulent behaviour in mouse and bovine infection models. the aim of this study was to determine the safety and efficacy of nc-spain 1 h when used as a vac ... | 2012 | 22913428 |
| toxoplasmosis in tammar wallabies (macropus eugenii) in the budapest zoo and botanical garden (2006-2010). | smaller macropodid species (commonly referred to as wallabies) are extremely susceptible to toxoplasmosis: in most cases, infection with toxoplasma gondii leads to death within a short time. between june 2006 and july 2010, t. gondii was detected by immunohistochemical examination in six tammar wallabies (macropus eugenii) that died in the budapest zoo and botanical garden; in another four specimens histopathology revealed t. gondii-like organisms (which could not be differentiated from neospora ... | 2012 | 22903081 |
| rabbit antibodies against toxoplasma hsp20 are able to reduce parasite invasion and gliding motility in toxoplasma gondii and parasite invasion in neospora caninum. | toxoplasma gondii hsp20 is a pellicle-associated functional chaperone whose biological role is still unknown. hsp20 is present in different apicomplexan parasites, showing a high degree of conservation across the phylum, with neospora caninum hsp20 presenting an 82% identity to that of t. gondii. hence rabbit anti-t. gondii hsp20 serum was able to recognize the n. caninum counterpart. interestingly, both n. caninum and t. gondii hsp20 localized to the inner membrane complex and to the plasma mem ... | 2012 | 22902747 |
| prevalence and risk factors associated with neospora caninum infection in dairy herds in jordan. | a cross-sectional study was carried out to determine the seroprevalence and to identify risk factors associated with neospora caninum infection in 62 dairy herds (n = 671 cows) in jordan between january and june 2007. information regarding herd management was recorded through personal interviews with farmers. antibodies against n. caninum were detected using an indirect elisa test. chi-square analysis and multivariable logistic regression model were used to identify risk factors associated with ... | 2013 | 22869339 |
| label-free molecular analysis of live neospora caninum tachyzoites in host cells by selective scanning raman micro-spectroscopy. | a selective scanning method was used to measure spatially resolved raman spectra of live neospora caninum tachyzoites colonizing human brain microvascular-endothelial cells. the technique allowed the detection of nucleic acids, lipids and proteins linked to the parasites and their cellular micro-environment at ∼10× shorter acquisition time compared to raster scanning. | 2012 | 22842554 |
| occurrence of anti-neospora caninum antibodies in dogs in rural areas in minas gerais, brazil. | the aim of this study was to determine the frequency of anti-neospora caninum antibodies and risk factors associated with seropositivity in 240 dogs from rural areas of the lavras, belo horizonte and nanuque regions, state of minas gerais, brazil. the indirect fluorescence antibody test (ifat) with a cutoff of 50 was used to assess the frequency of seropositive dogs. the risk factor analysis was performed using the chi-square test (χ2) and multiple logistic regression. the frequency of seroposit ... | 2013 | 22832759 |
| occurrence of anti-toxoplasma gondii and anti-neospora caninum antibodies in cats with outdoor access in são luís, maranhão, brazil. | the present study aimed to investigate the frequency of anti-toxoplasma gondii and anti-neospora caninum antibodies in cats with outdoor access in são luís, maranhão, brazil. the presence of igg anti-t. gondii and anti-n. caninum antibodies was tested using the indirect immunofluorescent antibody test (ifat). igg anti-t. gondii and anti-n. caninum antibodies were detected in 101 (50.5%) and 54 (27%) sampled cats, respectively. the titers of anti-t. gondii antibodies ranged from 40 (cut-off) to 2 ... | 2013 | 22832749 |
| neospora caninum and leishmania infantum co-infection in domestic dogs (canis familiaris) in meshkin-shahr district, northwestern iran. | mediterranean visceral leishmaniasis (mvl) is an infectious disease that affects both human and animals. domestic dogs (canis familiaris) are principal reservoir hosts of mvl caused by leishmania infantum. dogs are definitive hosts for neospora caninum and a risk factor for infecting intermediate hosts. the immunosuppression caused by visceral leishmaniasis (vl) can promote the occurrence of co-infections with other agents such as neosporosis. this study aimed to determine the frequency of co-in ... | 2011 | 22808419 |
| production, characterization and applications for toxoplasma gondii-specific polyclonal chicken egg yolk immunoglobulins. | toxoplasma gondii may cause abortions, ocular and neurological disorders in warm-blood hosts. immunized mammals are a wide source of hyperimmune sera used in different approaches, including diagnosis and the study of host-parasite interactions. unfortunately, mammalian antibodies present limitations for its production, such as the necessity for animal bleeding, low yield, interference with rheumatoid factor, complement activation and affinity to fc mammalian receptors. igy antibodies avoid those ... | 2012 | 22808150 |
| seroprevalence of toxoplasma gondii and neospora caninum in dogs from korea. | toxoplasma gondii and neospora caninum are closely related protozoan parasites, they share many common hosts, and can cause neurological diseases in dogs. dogs can have close contacts with humans and livestock and therefore they can act as reservoirs of these parasites. the aim of this study was to survey the seroprevalence of antibodies against t. gondii and n. caninum and their co-infection rate in dogs in korea. in total, sera from 553 domestic dogs were collected from different breeds, sexes ... | 2012 | 22807008 |
| serological evidence of besnoitia spp. infection in canadian wild ruminants and strong cross-reaction between besnoitia besnoiti and besnoitia tarandi. | bovine besnoitiosis, caused by besnoitia besnoiti, is considered to be emergent in europe and responsible for severe economic losses due to the chronic and debilitating course of the disease but has not been reported in north america. besnoitia tarandi is a related species and it has been reported in reindeer and caribou from different locations of the arctic pole, including north america. diagnosis of clinical besnoitiosis is largely based on the recognition of dermal grossly visible tissue cys ... | 2012 | 22795672 |
| characterization of the inflammatory response in the uteri of cows infected naturally by neospora caninum. | bovine neosporosis manifests during gestation, when the fetus may be vulnerable to pathogens. experimental studies of neospora caninum-infected cattle have been performed, but little is known about the in-utero inflammatory response. the aim of this study was to characterize the inflammatory response in the uteri of pregnant and non-pregnant cows infected naturally with n. caninum. four groups of seven animals were studied. two groups consisted of n. caninum-seropositive pregnant and non-pregnan ... | 2013 | 22784781 |
| performance of a commercial serum elisa for the detection of antibodies to neospora caninum in whole and skim milk samples. | control of neospora caninum infection in cattle depends on specific, ante-mortem detection of infected animals and limiting their use as breeding stock or by culling. the objective of the present study was to determine appropriate cut-off values and diagnostic performance of a milk elisa test using whole and skim milk in a commercial serum elisa test (idexx neospora ab). serum and milk samples were obtained from a total of 475 lactating cows from two herds with and two herds without a previous h ... | 2012 | 22770889 |
| detection and molecular analysis of toxoplasma gondii and neospora caninum from dogs with neurological disorders. | toxoplasma gondii and neospora caninum are related apicomplexa parasites responsible for systemic diseases in many species of animals, including dogs. | 2012 | 22760137 |
| neospora caninum in estonian dairy herds in relation to herd size, reproduction parameters, bovine virus diarrhoea virus, and bovine herpes virus 1. | cows infected with the tissue parasite neospora caninum (nc) are more likely to abort or give birth to calves with neurological disorders. the known infection routes are transplacentally and by consumption of oocysts shed by the definitive host, the dog. it has been hypothesised, that dormant stages of persistent nc infection may be reactivated by immunosuppression mechanisms such as pathogenic invasions as bovine herpes virus 1 (bhv1) and bovine virus diarrhoea virus (bvdv). the study was set t ... | 2012 | 22721941 |
| seroprevalence of toxoplasma gondii and neospora caninum infection in dairy cows in subtropical southern china. | toxoplasma gondii and neospora caninum are closely related protozoan parasites which cause lowered production and increased abortion in dairy cows. the aim of the present study was to determine the seroprevalence of t. gondii and n. caninum infection in dairy cows in the guangxi zhuang autonomous region (gzar), subtropical southern china. in total, 875 serum samples were collected from the tail veins of dairy cows in 6 main dairy cow-rearing districts of 4 administrative cities in gzar. the samp ... | 2012 | 22717118 |
| multivariate model for the assessment of risk of fetal loss in goat herds. | the observational study was carried out in a population of polish breeding goats in 2007 to determine the prevalence of fetal loss and identify risk factors contributing to its occurrence. the multivariate model allowing to predict the risk of the occurrence of fetal loss in a herd in a study population was developed. data on the occurrence of fetal loss, as well as of 28 hypothesized risk factors were collected from goat owners using standardized questionnaire during face-to-face reviews on far ... | 2012 | 22708360 |
| molecular frequency and isolation of cyst-forming coccidia from free ranging chickens in bahia state, brazil. | the toxoplasmatinae parasites toxoplasma gondii, neospora caninum and hammondia spp. have carnivores as definitive hosts that shed the parasite oocysts in their feces. birds that feed directly from the soil, such as chickens, are exposed to infection and may serve as indicators of the presence of the parasite in the environment and as a source of infection for other animals. the aims of this study were to determine the frequency of infection by these parasites in free ranging chickens, to test w ... | 2012 | 22673105 |
| adult-onset nemaline myopathy in a dog presenting with persistent atrial standstill and primary hypothyroidism. | a nine-year-old neutered female mixed breed dog presented for evaluation following a five-day history of lethargy, inappetence, weakness, abdominal distension and generalised muscle atrophy. persistent vatrial standstill with a junctional rhythm was identified on electrocardiogram. echocardiogram identified moderate dilation of all cardiac chambers and mild thickening of the mitral and tricuspid valves. serology was negative for neospora caninum and toxoplasma gondii. permanent pacemaker implant ... | 2012 | 22647214 |
| prevalence of neospora caninum antibodies in dairy cattle and water buffaloes and associated abortions in the plateau of southern peninsular india. | a seroprevalence study of bovine neosporosis was conducted among 1,927 dairy cattle and 341 water buffaloes from karnataka and andhra pradesh states in plateau of southern peninsular india by employing competitive enzyme-linked immunosorbent assay. overall, 12.61 and 9.97 % sera samples were found positive for the presence of neospora caninum antibody, respectively, among cattle and water buffaloes. out of 1,927 sera samples from cattle, 912 and 1,015 samples were collected from unorganized and ... | 2013 | 22644733 |
| low efficacy of ncgra7, ncsag4, ncbsr4 and ncsrs9 formulated in poly-ε-caprolactone against neospora caninum infection in mice. | the protective efficacy of vaccination with neospora caninum recombinant antigens was evaluated in balb/c pregnant and non-pregnant mouse models of neosporosis. a major immunodominant dense granule protein (ncgra7) and three bradyzoite-specific surface antigens (ncsag4, ncbsr4 and ncsrs9) were expressed in escherichia coli and encapsulated within poly-ε-caprolactone (pcl) nanoparticles for the first time. good efficiencies of entrapment (greater than 50%) were obtained for all encapsulated prote ... | 2012 | 22640942 |
| serosurvey of antibodies against toxoplasma gondii and neospora caninum in white-tailed deer from northern mexico. | the objective of this study was to determine the seroprevalence of toxoplasma gondii and neospora caninum in white-tailed deer from northern mexico. sera from 532 white-tailed deer (odocoileus virginianus) from three northern states of mexico were assayed for antibodies to t. gondii by elisa and western blot. from these samples, 368 were available to test for n. caninum antibodies by elisa. the overall prevalence for t. gondii antibodies was 13.9% (74/532; ci(95) 11-17) and for n. caninum 8.4% ( ... | 2012 | 22633992 |
| the neospora caninum-spain 7 isolate induces placental damage, fetal death and abortion in cattle when inoculated in early gestation. | the nc-spain 7 isolate of neospora caninum, which was newly obtained from an asymptomatic congenitally infected calf, demonstrated a similar virulence as nc-1 strain in mouse models. the aim of this study was to characterize the pathogenesis of nc-spain 7 isolate in cattle after experimental infection at 65 days of gestation. for this purpose, thirteen pregnant heifers were divided into three groups as follows: group a: 7 heifers inoculated with 1 × 10(8) tachyzoites of nc-spain 7 isolate; group ... | 2012 | 22621962 |
| a study of some infectious causes of reproductive disorders in cattle owned by resource-poor farmers in gauteng province, south africa. | two hundred and thirty-nine cattle from gauteng province in south africa were tested for various pathogens causing reproductive diseases includingbovine viral diarrhoea/mucosal disease (bvd/md) virus, infectious bovine rhinotracheitis/infectious pustular vulvovaginitis (ibr/ipv) virus, neospora caninum and brucella abortus usingvarious tests. for bvd/md virus, 49.37% tested positive, 74.47% for ibr/ipv virus, 8.96% for neospora caninum and 3.8% for brucella abortus. the result for brucella abort ... | 2011 | 22616434 |