Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| frequency of horizontal and vertical transmission for sarcocystis cruzi and neospora caninum in dairy cattle. | sarcocystis cruzi and neospora caninum infections in cattle are common throughout the world, and cause important economical losses. n. caninum can be transmitted horizontally by ingestion of oocysts or vertically from the infected dam to the fetus via the placenta. vertical transmission for s. cruzi is infrequent and horizontal transmission is considered the most important route of infection. the objectives of this study were to evaluate the frequency of horizontal and vertical transmission for ... | 2009 | 19070964 |
| effects of a transferring antibody against neospora caninum infection in a murine model. | we investigated the roles of a transferring antibody against neospora caninum infection based on a murine model using recombinant vaccinia virus carrying ncsrs2 gene. higher levels of anti-ncsrs2 antibody were detected in surviving offspring from vaccinated dams than controls while the transferring anti-ncsag1 antibody was detected in the surviving offspring from unvaccinated dams. after mating, the female mice with transferring antibody against n. caninum were challenged with the parasites in m ... | 2009 | 19056179 |
| factors affecting plasma prolactin concentrations throughout gestation in high producing dairy cows. | the aim of the present study was to investigate possible relationships between plasma concentrations of prolactin and the following factors throughout gestation in lactating dairy cows: photoperiod, season, milk production, neospora caninum-seropositivity, twin pregnancy, and plasma concentrations of pregnancy associated glycoproteins-1 (pag-1), progesterone and cortisol. pregnancy was diagnosed by trans-rectal ultrasonography on day 40 post-insemination and by palpation per rectum on days 90, 1 ... | 2009 | 19036554 |
| comparison of two pcr protocols for the detection of neospora caninum dna in rodents. | the objective of the present study was, due to increasing interest in the epidemiological role of small mammals as potential reservoir of neospora caninum, to compare two different pcr protocols for the diagnosis of n. caninum in rodents. we tested tissue samples from 50 house mice (mus musculus), 50 rats (rattus norvegicus) and 35 field mice (apodemus sylvaticus). two different pcr protocols based on primer pairs, np4-np7 and np6plus-np21plus, were used for diagnosis on these samples. while the ... | 2009 | 19036521 |
| first isolation of neospora caninum from the faeces of a dog from portugal. | we report the in vitro isolation of neospora caninum from the faeces of a naturally infected 8-year-old male stray boxer from portugal. vero cell cultures were infected using parasite stages obtained after oral inoculation of gamma-interferon knockout mice with 10(2) sporulated oocysts. the isolate was identified by microscopical examination, as well as histological, immunological and molecular methods including a dna-microsatellite-based typing technique, and was subsequently named nc-p1. the d ... | 2009 | 19036520 |
| looks can deceive: molecular identity of an intraerythrocytic apicomplexan parasite in australian gliders. | two yellow-bellied gliders (petaurus australis) had an intraerythrocytic parasite closely related to the cyst-forming coccidia (apicomplexa: sarcocystidae). the parasitaemia persisted for 3 months or more but was observed to clear within 3 years in captivity. the parasite appears not to significantly debilitate its infected host. traditionally, using morphological identification, the intraerythrocytic parasite would have been classified within the hepatozoon species typically found in red blood ... | 2009 | 19028015 |
| neospora caninum associated with epidemic abortions in dairy cattle: the first clinical neosporosis report in turkey. | neospora caninum, a protozoan parasite, has been considered as one of the most important etiological agents responsible for abortion in dairy cattle throughout the world since it was first identified in dogs in 1988. in this report, characteristics of neosporosis, detected in a dairy cow ranch having epidemic abortions as high as 18.4%, were described. blood samples were collected from 25 infertile or aborted dairy cattle, 6 calves born in 2006 and 40 heifers that were born in 2005 and raised in ... | 2009 | 19028014 |
| pigeons (columba livia) are a suitable experimental model for neospora caninum infection in birds. | neospora caninum infections in chickens have been recently described by epidemiological and experimental approaches, and these birds may be considered natural intermediate hosts of the parasite. it has been postulated that other bird species might perform this role in wildlife as well. to better understand the sylvatic life cycle of n. caninum, further studies are required. in that sense, this work aimed to observe infection kinetics in pigeons experimentally infected with n. caninum. experiment ... | 2009 | 19027237 |
| isolation and characterization of a bovine isolate of neospora caninum with low virulence. | neospora caninum tachyzoites were isolated from the brain of an asymptomatic naturally infected calf with precolostral-specific antibodies. the new isolate, named nc-spain 1h, was identified as a member of the n. caninum species based on its internal transcribed spacer 1 (its-1) sequence and was genetically characterized using microsatellite markers. multilocus analysis showed that nc-spain 1h was genetically different from other n. caninum isolates. we compared the in vitro tachyzoite yield and ... | 2009 | 19027235 |
| spatial analysis of neospora caninum distribution in dairy cattle from sweden. | the national herd prevalence and spatial distribution of neospora caninum infected dairy herds in sweden were investigated. the study was based on a bulk milk survey comprising samples from 2,978 herds. test-positive herds were found in all parts of sweden and the overall prevalence of test-positive herds was 8.3% (95% confidence interval = 7.3-9.3%). the presence of spatial autocorrelation was tested using the moran's i test. possible clusters of test-positive herds were identified by applying ... | 2008 | 19021107 |
| neospora caninum, potential cause of abortions in dairy cows: the current serological follow-up in slovakia. | neosporosis is considered to be a contributing risk factor for abortions in dairy cows and other farm animals and has negative economic impact on their breeding. in respect of the rapid spread of neosporosis in herds throughout the world, our aim was to detect the prevalence of anti-neospora antibodies in cows post-abortion (pa) (pa group n=716) and in cows without any reproduction problems (control group n=247) on large breeding farms from eastern slovakia. the overall mean seropositivity in pa ... | 2009 | 19019551 |
| seroprevalence of neospora caninum antibodies in beef cattle in three southern states of mexico. | the aims of the present study were to examine the seroprevalence of neosporosis in beef herds from three southern states of mexico and determine the association with several risk factors. a cross-sectional serological survey for neospora caninum was carried out by sampling a total of 596 animals from 31 herds in chiapas, veracruz, and yucatan states and tested using an elisa assay (idexx) to detect anti-n. caninum antibodies. the overall prevalence was 11.6%, (95% ci: 0.93-0.14), however, the pr ... | 2009 | 19016341 |
| proteomes and transcriptomes of the apicomplexa--where's the message? | the apicomplexa have some of the most comprehensive and integrated proteome datasets of all pathogenic micro-organisms. coverage is currently at a level where these data can be used to help predict the potential biological function of proteins in these parasites, without having to defer to measurement of mrna levels. transcriptomic data for the apicomplexa (microarrays, expressed sequence tag (est) collections, serial analysis of gene expression (sage) and massively parallel signature sequencing ... | 2009 | 18996390 |
| molecular approaches to diversity of populations of apicomplexan parasites. | apicomplexan parasites include many parasites of importance either for livestock or as causative agents of human diseases. the importance of these parasites has been recognised by the european commission and resulted in support of the cost (cooperation in science and technology) action 857 'apicomplexan biology in the post-genomic era'. in this review we discuss the current understanding in 'biodiversity and population genetics' of the major apicomplexan parasites, namely the eimeria spp., crypt ... | 2009 | 18983997 |
| isolation and genetic characterization of neospora caninum from asymptomatic calves in spain. | neospora caninum is a cyst-forming parasite that causes abortion in cattle. despite this parasite's ubiquitous distribution and wide host range, the number of n. caninum isolates obtained to date is limited. in vitro isolation of the parasite is arduous and often unsuccessful. in addition, most isolates have been obtained from clinically affected hosts and therefore could be biased towards more virulent isolates. in this report, an improved isolation approach from transplacentally infected newbo ... | 2008 | 18980700 |
| studies on neospora caninum dna detection in the oocytes and embryos collected from infected cows. | neosporosis is a major cause of abortion in cattle over the world. one of the methods of preventing vertical transmission within the herd is to avoid breeding replacement heifers from infected dams. another procedure suggested and recommended by the international embryo transfer society (iets) is embryo transfer (et) from infected dams into uninfected recipients. oocytes and embryos taken from seropositive cows were examined for the presence of neospora caninum dna. a modified pcr protocol using ... | 2008 | 18977601 |
| natural breeding with bulls experimentally infected with neospora caninum failed to induce seroconversion in dams. | four bulls and 56 heifers seronegative to neospora caninum were used to determine the feasibility of venereal transmission in bovine neosporosis under natural conditions. bulls were experimentally infected with 10(8) live n. caninum tachyzoites. two of them with the nc-1 isolate and the other two with the nc-spain-7 isolate. after 13 months of initial infection, each bull was re-infected with the same isolate and dose. the experiments were carried out from march to september during 2006 and 2007 ... | 2009 | 18962878 |
| infection of primary canine duodenal epithelial cell cultures with neospora caninum. | according to current knowledge, sexual development of the apicomplexan parasite neospora caninum takes place in the canine intestine. however, to date there is no information on the interaction between the parasite and the canine intestinal epithelium, and, next to the clinical and in vivo research tools, an in vitro model comprised of canine intestinal cells infected with n. caninum would be very helpful for investigations at the cellular level. following the isolation of cells of neonatal cani ... | 2009 | 18821824 |
| usefulness of rncgra7- and rncsag4-based elisa tests for distinguishing primo-infection, recrudescence, and chronic bovine neosporosis. | bovine reproductive failure caused by the parasite neospora caninum is a major problem and is responsible for severe economic losses worldwide. currently, appropriate control measures depend on the predominant transmission route in a particular herd. therefore, the development of diagnostic tools capable of discriminating between primo-infection, recrudescence, re-infection, and chronic infection is a major challenge in the serodiagnosis of bovine neosporosis. here, two recombinant protein-based ... | 2008 | 18814972 |
| a four year longitudinal sero-epidemiology study of neospora caninum in adult cattle from 114 cattle herds in south west england: associations with age, herd and dam-offspring pairs. | neosporosis caused by the protozoan parasite neospora caninum, is an economically important cause of abortion, stillbirth, low milk yield, reduced weight gain and premature culling in cattle. consequently, a seroepidemiological study of n. caninum antibodies was conducted in england with 29,782 samples of blood taken from 15,736 cattle from 114 herds visited on three occasions at yearly intervals. herds were categorised into lower (< 10%) and higher (> or = 10%) median herd seroprevalence. hiera ... | 2008 | 18793402 |
| evaluation of recombinant proteins of neospora caninum as vaccine candidates (in a mouse model). | abortion, resulting from infections by the parasite neospora caninum, is a major cause of economic loss to both the dairy and beef industries of cattle-producing countries of the world. vaccination as a means of preventing abortion and/or infection represents a viable control strategy; indeed a commercial vaccine is available in some countries, albeit of unknown efficacy. the commercial vaccine is based on inactivated tachyzoites of n. caninum but other approaches based on lysates and recombinan ... | 2008 | 18789996 |
| serological survey of neospora caninum infection among dogs in japan through species-specific elisa. | a seroepidemiological survey of neospora caninum infection among dogs in japan was conducted using species-specific enzyme-linked immunosorbent assay with recombinant surface antigen (nc-sag1t). among 1,206 dogs examined, 126 dogs (10.4%) from 30 prefectures from hokkaido to okinawa were positive to n. caninum infections, which were more frequently detected in females than males. siberian huskies showed the highest positive rate compared with the other breeds. dogs with pyometra and diabetes mel ... | 2008 | 18772569 |
| prevalence of toxoplasma gondii and neospora caninum infections in sheep from federal district, central region of brazil. | serum samples from 1028 sheep were collected from 32 herds within federal district, in the central region of brazil. the samples were examined by indirect fluorescent antibody test (ifat) using sera diluted 1:64 and 1:50 as cut-off values for the detection of antibodies against toxoplasma gondii and neospora caninum, respectively. the observed prevalence for t. gondii infection was 38.22% (26.81%<ci 0.95<49.62%), and the titers ranged from 64 to 65536. the observed prevalence for n. caninum infe ... | 2009 | 18726165 |
| occurrence of antibodies to neospora caninum and toxoplasma gondii in dogs from pernambuco, northeast brazil. | a serological survey was carried out to assess the occurrence of anti-neospora caninum antibodies in dogs from the state of pernambuco. a total of 625 serum samples of dogs (289 from paulista, 168 from amaraji and 168 from garanhuns) were tested by an immunofluorescence antibody assay for the detection of anti-n. caninum antibodies. a total of 177 (28.3%; ic 95%, 24.9-32.1) samples were positive. the seropositivity rates found in paulista, amaraji and garanhuns were 26% (ic 95%, 21-31.4), 26.2% ... | 2008 | 18723288 |
| seroepidemiological study of neospora caninum infection in dogs found in dairy farms and urban areas of aguascalientes, mexico. | the objective of this study was to describe the seroepidemiology of neospora caninum infection in dogs in close contact with dairy cattle and dogs from urban areas of aguascalientes, mexico, as well as to estimate the possible association between age, gender and size of these groups and seroprevalence. sera were obtained from samples of 152 dogs in dairy farms and 116 in the urban area, and were subjected to elisa test. general seroprevalence reached 32%, while the seroprevalence in farm dogs (4 ... | 2008 | 18722716 |
| neospora caninum: detection in wild rabbits and investigation of co-infection with toxoplasma gondii by pcr analysis. | neospora caninum is an important pathogen of cattle causing significant economic loss. there is much current interest in wild animal reservoirs for this parasite. the role of the rabbit in this is currently unknown. dna samples from the brains of wild rabbits (oryctolagus cuniculus) collected from the malham area of the yorkshire dales were investigated by species-specific pcr for the presence of n. caninum and toxoplasma gondii. we found prevalences of n. caninum of 10.5% (6/57) and t. gondii o ... | 2008 | 18703054 |
| risk factors associated with mycobacterium avium subspecies paratuberculosis seropositivity in canadian dairy cows and herds. | our objective was to determine the risk factors associated with the seroprevalence of mycobacterium avium subspecies paratuberculosis (map) in a large number of randomly selected canadian dairy herds, controlling for important confounding variables and co-infections with bovine leukemia virus (blv), bovine viral diarrhea virus (bvdv) and neospora caninum (nc). serum samples from 30 randomly selected cows, where available, in 315 herds from seven provinces were tested for antibodies against blv, ... | 2009 | 18692926 |
| the role of neospora caninum and toxoplasma gondii in spontaneous bovine abortion in argentina. | the aim of the present work was to evaluate the role of neospora caninum and toxoplasma gondii infections in spontaneous bovine abortions in argentina. based on histopathological results, 70 presumptive cases of apicomplexan protozoal abortion from a total of 666 cases of spontaneous bovine abortion submitted to the national institute of agrarian technology, balcarce, from 1999 to 2007 were included in this study. n. caninum infection was diagnosed by an indirect fluorescent antibody test (ifat) ... | 2008 | 18691819 |
| generation and infection of bovine pbmc-derived dendritic cells with neospora caninum. | 2008 | 18683066 | |
| toxoplasma gondii rhoptry discharge correlates with activation of the early growth response 2 host cell transcription factor. | toxoplasma gondii is a ubiquitous apicomplexan parasite that can cause severe disease in fetuses and immune-compromised patients. rhoptries, micronemes, and dense granules, which are secretory organelles unique to toxoplasma and other apicomplexan parasites, play critical roles in parasite growth and virulence. to understand how these organelles modulate infected host cells, we sought to identify host cell transcription factors triggered by their release. early growth response 2 (egr2) is a host ... | 2008 | 18678671 |
| toxoplasma rhoptries: unique secretory organelles and source of promising vaccine proteins for immunoprevention of toxoplasmosis. | toxoplasma gondii is an obligate intracellular protozoan parasite classified in the phylum apicomplexa, which includes numerous notable human and animal pathogens (plasmodium species, cryptosporidium species, neospora caninum, etc.). the invasive stages of apicomplexans are characterized by the presence of an apical complex composed of specialized cytoskeletal and secretory organelles, including rhoptries. rhoptries, unique apical secretory organelles shared exclusively by all apicomplexan paras ... | 2008 | 18670609 |
| seroprevalence of neospora caninum and toxoplasma gondii in black-tailed deer (odocoileus hemionus columbianus) and mule deer (odocoileus hemionus hemionus). | deer are considered important intermediate hosts for the coccidian parasites, toxoplasma gondii and neospora caninum. antibodies to n. caninum and t. gondii were determined in sera of 42 mule deer (odocoileus hemionus hemionus) and 43 black-tailed deer (odocoileus hemionus columbianus) from washington state, usa, using direct agglutination test with specific antigens. a titer of 1:25 was considered diagnostic for both parasites. n. caninum antibodies were found in 7 of 42 mule deer and 8 of 43 b ... | 2008 | 18650016 |
| sarcocystis neurona: molecular characterization of enolase domain i region and a comparison to other protozoa. | sarcocystis neurona causes protozoal myeloencephalitis and has the ability to infect a wide host range in contrast to other sarcocystis species. in the current study, five s. neurona isolates from a variety of sources, three sarcocystis falcatula, one sarcocystis dasypi/s. neurona-like isolate, and one besnoitia darlingi isolate were used to compare the enolase 2 gene segment containing the domain i region to previously sequenced enolase genes from neospora caninum, neospora hughesi, toxoplasma ... | 2008 | 18625501 |
| the differentiation of transcription between tachyzoites and bradyzoites of in vitro cultured neospora caninum. | interconversion between tachyzoites and bradyzoites of neospora caninum plays a pivotal role in transmission of the parasite. although significant efforts have been made toward understanding the mechanisms that trigger and control stage conversion of the parasite, little is known about this process. we used annealing control primer (acp)-based polymerase chain reaction (pcr) technique to characterize the differences in transcription between tachyzoites and bradyzoites of n. caninum. the in vitro ... | 2008 | 18618147 |
| canine and bovine neospora caninum control sera examined for cross-reactivity using neospora caninum and neospora hughesi indirect fluorescent antibody tests. | neospora caninum is a well known protozoan parasite of domestic and wild animals. neospora hughesi is a closely related protozoan with an unknown life cycle, host range, and infection prevalence. many serologic surveys of n. caninum have been performed without consideration of potential cross-reactions with n. hughesi, which could confound results. the aim of this study was to investigate whether postexposure sera from animals experimentally infected with n. caninum exhibit significant reactivit ... | 2009 | 18613752 |
| toxoplasma gondii and neospora caninum antibodies in dogs from grenada, west indies. | toxoplasma gondii and neospora caninum are structurally similar parasites with many common hosts. the prevalence of antibodies to t. gondii and n. caninum was determined in sera from dogs in grenada, west indies. using a modified agglutination test, antibodies to t. gondii were found in 52 (48.5%) of the 107 dogs, with titers of 1:25 in 17, 1:50 in 19, 1:100 in 7, 1:1,600 in 5, and 1:3,200 or higher in 4. seroprevalence increased with age from 2.2% in dogs <6 mo old to 18.9% in dogs older than 2 ... | 2008 | 18605807 |
| occurrence of neospora caninum antibodies in capybaras (hydrochaeris hydrochaeris) from são paulo state, brazil. | capybara (hydrochaeris hydrochaeris) is a large rodent distributed throughout tropical america. antibodies to neospora caninum in 213 feral capybaras from 11 counties of the state of são paulo, brazil, were assessed using the indirect immunofluorescent antibody test (titer >or=1:25) and found in 20 (9.4%), with titers of 1:25 in 4, 1:50 in 7, and 1:100 in 9 animals. this is the first report of occurrence of n. caninum antibodies in capybaras. | 2008 | 18605804 |
| [epidemiological and financial considerations for the control of neospora caninum on swiss dairy farms]. | neospora caninum is widely recognized as one of the most important abortifacients in cattle and causes substantial financial losses to bovine livestock production. this study aimed to calculate the losses caused by n. caninum on swiss dairy farms and to evaluate the efficacy and profitability of the control strategies culling, not breeding replacements and chemotherapy of calves on farm level. three different farm sizes with high, medium and low herd prevalences were defined. epidemiological and ... | 2008 | 18605018 |
| the differential protein expression profiles and immunogenicity of tachyzoites and bradyzoites of in vitro cultured neospora caninum. | we report a study on the variations in the protein expression profiles of tachyzoites and bradyzoites of neospora caninum. the in vitro stage conversion of n. caninum-infected vero cells was induced by continuous treatment of infected cultures with 70 mum sodium nitroprusside (snp) for up to 9 days. the stage conversion indicated by the expression of the bradyzoite-specific antigen bag1 was analyzed by immunofluoresence assay. morphological changes between tachyzoites and bradyzoites and localiz ... | 2008 | 18597117 |
| influence of neospora caninum infection in balb/c mice during pregnancy in post-natal development. | the influence of neospora caninum infection during pregnancy on the post-natal period has been poorly investigated. in a previous study, we suggested that infection with n. caninum during pregnancy could affect the normal post-natal development of the offspring. for this reason, in the present work we evaluated the influence of n. caninum infection in pregnant balb/c mice at days 0, 7 and 14 of gestation (groups a, b and c, respectively) on the post-natal development of the offspring from birth ... | 2008 | 18586405 |
| serological survey of toxoplasma gondii in wild waterfowl in chukotka, kamchatka, russia and hokkaido, japan. | antibodies to toxoplasma gondii were assayed by elisa in 22 experimentally inoculated domestic ducks. in addition, a serological assay was carried out at obihiro, hokkaido, japan, in 2004 and 2005, on 221 wild ducks of 5 species: anas platyrhynchus (n = 111); a. poecilorhyncha (n = 27); a. acuta (n = 58); a. penelope (n = 16); and a. crecca (n = 9). assays were also conducted using sera from 197 wild geese of 2 species, i.e., anser albifrons (n = 162) and ans. fabalis (n = 35). birds were collec ... | 2008 | 18576786 |
| neospora caninum antibodies in wild carnivores from spain. | serum samples from 251 wild carnivores from different regions of spain were tested for antibodies to neospora caninum by the commercial competitive screening enzyme linked immunosorbent assay (c-elisa) and confirmed by neospora agglutination test (nat) and/or by indirect fluorescent antibody test (ifat). samples with antibodies detected by at least two serological tests were considered seropositive. antibodies to n. caninum were found in 3.2% of 95 red foxes (vulpes vulpes); in 21.4% of 28 wolve ... | 2008 | 18556128 |
| disease threats to the endangered iberian lynx (lynx pardinus). | the iberian lynx, (lynx pardinus), is the most endangered felid in the world. to determine whether sympatric carnivores are reservoirs of pathogens posing a disease risk for the lynx, evidence of exposure to 17 viral, bacterial and protozoan agents was investigated in 176 carnivores comprising 26 free-living lynx, 53 domestic cats, 28 dogs, 33 red foxes (vulpes vulpes), 24 egyptian mongooses (herpestes ichneumon), 10 common genets (genetta genetta) and 2 eurasian badgers (meles meles) in the are ... | 2009 | 18555712 |
| [occurrence of antibodies anti-neospora caninum in dogs of botucatu range micro region, state of são paulo, brazil]. | neosporosis is a parasitic disease caused by a protozoon neospora caninum, which is recognized as an important cause of bovine abortion and canine neuropathy. considering the isolation of n. caninum and the frequent serology in bovines of our region, the objectives of the present trial were to evaluate the occurrence of antibodies anti-n. caninum in dogs of the botucatu range micro region, in the state of são paulo, and its association with gender, age and origin (urban area--exclusively from th ... | 2008 | 18554432 |
| ncgra2 as a molecular target to assess the parasiticidal activity of toltrazuril against neospora caninum. | the treatment of neospora caninum infection in the bovine host is still at an experimental stage. in contrast to the in vivo situation, a wide range of compounds have been intensively investigated in cell-culture-based assays. tools to demonstrate efficacy of treatment have remained conventional including morphological and cell biological criteria. in this work, we present a molecular assay that allows the distinction between live and dead parasites. live parasites can be detected by measuring t ... | 2008 | 18549517 |
| frequency of detectable serum igg concentrations in precolostral calves. | to determine the prevalence of detectable serum igg concentrations in calves prior to ingestion of colostrum and to assess whether a detectable igg concentration was related to dam parity, calf birth weight, calf sex, season of calving, or infectious agents that can be transmitted transplacentally. | 2008 | 18518660 |
| lack of serologic evidence of neospora caninum in humans, england. | retrospective testing of 3,232 serum samples from the general population and 518 serum samples from a high-risk group showed no evidence of human exposure to neospora caninum in england. results were obtained by using immunofluorescence antibody testing and elisa to analyze frequency distribution. | 2008 | 18507920 |
| detection of neospora caninum in dog organs using real time pcr systems. | neospora caninum is a parasite responsible for paresis in dogs. the dog can harbour enkysted parasites in several organs. the detection of n. caninum was performed using 3 different real time pcr systems all amplifying the nc5 dna region. one system was based on sybrgreen, one on plexor technology and the last on taqman probe. comparison of the three methods indicated that the detection limit was 1 equivalent genome on pure dna but that this detection limit increased in the presence of foreign d ... | 2008 | 18502585 |
| seroprevalence of neospora caninum, toxoplasma gondii and sarcocystis sp. in llamas (lama glama) from jujuy, argentina. | llamas (lama glama) are south american camelids described as intermediate hosts of neospora caninum, toxoplasma gondii and sarcocystis aucheniae. due to the potential role of these protozoan infections as a cause of economic losses, the aim of this study was to determine the seroprevalence for t. gondii, n. caninum and sarcocystis sp. in llamas from argentina. serum samples from 308 llamas (>2 years old) were collected between 2005 and 2007. a total of 55 farms located in six departments of juju ... | 2008 | 18495344 |
| vaccination of mice with recombinant ncrop2 antigen reduces mortality and cerebral infection in mice infected with neospora caninum tachyzoites. | rhoptry antigens are involved in a variety of cellular functions related to host cell invasion, formation of the parasitophorous vacuole and parasite-host cell interplay. the cdna sequence of one of these antigens, ncrop2 was identified from neospora caninum expressed sequence tags (ests), amplified by reverse transcription-pcr, expressed in escherichia coli as a (his)(6)-tagged recombinant protein (recncrop2) and purified over ni(2+)-affinity chromatography. both recncrop2 and antibodies direct ... | 2008 | 18495126 |
| encephalitis in aborted bovine fetuses associated with bovine herpesvirus 1 infection. | brain tissue from 12 aborted bovine fetuses submitted to the wisconsin veterinary diagnostic laboratory revealed histologic lesions that consisted of glial nodules and variable degrees of mononuclear inflammation, microhemorrhage, neuronal necrosis, and cerebral cortical cavitation. a diagnosis of bovine herpesvirus 1 (bhv-1) abortion had been made in all of these cases through multiple testing modalities. brain tissue from 8 of the 12 fetuses was immunohistochemically stained with a monoclonal ... | 2008 | 18460615 |
| evolutionary origin of the protozoan parasites histone-like proteins (hu). | the histone-like proteins (hu) belong to a family of dna architectural proteins that stabilize nucleoprotein complexes. we found a putative hu protein (tgglmhmm_3045) in toxoplasma gondii genome that was homologous to the bacterial hu protein. this putative sequence was located in the scaffold tgg_995361 of the chromosome 10. the sequence included the prokaryotic bacterial histone-like domain, kfgslglrrrgervarnprt (id number ps00045). hu protein sequences were also found in plasmodium falciparum ... | 2008 | 18430986 |
| the development and evaluation of a nested pcr assay for detection of neospora caninum and hammondia heydorni in feral mouse tissues. | the development of a novel nested polymerase chain reaction is described and used for detecting the presence of neospora caninum and hammondia heydorni dna in dna extracted from feral rodent tissues. a unique strategy was used for design of an assay that could be adapted for detecting dna from more than one member of toxoplasmatinae simultaneously with a minimal number of additional steps. the level of sensitivity described for this assay is comparable to real time-pcr and other nested pcr assay ... | 2008 | 18420378 |
| seroprevalence of toxoplasma gondii and neospora caninum infection of cats in hungary. | blood samples were collected from 330 cats in hungary in order to evaluate their seroconversion to toxoplasma gondii and neospora caninum using the indirect fluorescent antibody test (ifat). the overall prevalence of toxoplasmosis was 47.6%, the prevalence being 22.4% among urban, 50% among suburban and 61.3% among rural animals. significantly more cats had high ifat titres (1:640 to 1:5120) in the countryside. female cats were more frequently infected with t. gondii than males (53.3% vs. 39.3%) ... | 2008 | 18401958 |
| models for experimental infection of dogs fed with tissue from fetuses and neonatal cattle naturally infected with neospora caninum. | three models were designed to investigate the development and enteroepithelial phase of neospora caninum in dogs, and to induce oocyst production by the parasite. in the first model, three dogs were fed raw fetal bovine tissue on two occasions. the bovine fetal tissue had been stored at 4 degrees c for 14 days and 16 days, respectively, and had tested positive for neosporosis using histopathology and immunohistochemistry. in the second model, nine dogs from the beginning of the experiment until ... | 2008 | 18395346 |
| seroprevalence of neospora caninum antibodies in dogs in india. | neospora caninum is one of most important causes of abortion in cattle worldwide, and dogs are an important risk factor for n. caninum infection in cattle. antibodies to n. caninum were determined in 184 (126 rural, 58 urban) dogs from the punjab state, india, using commercial monoclonal antibody-based competitive elisa and found in 16.8% of the animals. the prevalence of n. caninum antibodies was significantly higher in rural dogs (21.4%, 27 of 126) than city dogs (6.9%, 4 of 58). to our knowle ... | 2008 | 18372658 |
| host cells participate in the in vitro effects of novel diamidine analogues against tachyzoites of the intracellular apicomplexan parasites neospora caninum and toxoplasma gondii. | the in vitro effects of 19 dicationic diamidine derivatives against the proliferative tachyzoite stages of the apicomplexan parasites neospora caninum and toxoplasma gondii were investigated. four compounds (db811, db786, db750, and db766) with similar structural properties exhibited profound inhibition of tachyzoite proliferation. the lowest 50% inhibitory concentrations were found for db786 (0.21 microm against neospora and 0.22 microm against toxoplasma) and db750 (0.23 microm against neospor ... | 2008 | 18362190 |
| upregulation of cytokines is detected in the placentas of cattle infected with neospora caninum and is more marked early in gestation when fetal death is observed. | the protozoan parasite neospora caninum causes fetal death after experimental infection of pregnant cattle in early gestation, but the fetus survives a similar infection in late gestation. an increase in th1-type cytokines in the placenta in response to the presence of the parasite has been implicated as a contributory factor to fetal death due to immune-mediated pathological alterations. we measured, using real-time reverse transcription-pcr and enzyme-linked immunosorbent assay, the levels of ... | 2008 | 18362132 |
| effects of re-infection with neospora caninum in bulls on parasite detection in semen and blood and immunological responses. | three bulls with experimentally induced primary infection with neospora caninum were re-infected intravenously with 10(8) live n. caninum tachyzoites of the nc-1 isolate at 300 days post-infection to investigate the presence of n. caninum in semen and blood, and the associated immune responses. in parallel, three bulls with experimentally induced primary infection with n. caninum and three non-infected bulls were also monitored. re-infected and infected bulls showed an intermittent presence of n ... | 2008 | 18336895 |
| parasitic infections in dairy cattle around hanoi, northern vietnam. | in northern vietnam, dairy cattle are mainly managed in small-scale farms, where animals are kept confined and feeding occurs by cut and carry methods. in the present study the occurrence of parasitic infections was examined in five provinces around hanoi. a total of 201 farms were visited, and 334 stool and 239 blood samples were collected from calves younger than 3 months, animals between 3 and 24 months and adult cows. furthermore, 254 milk samples were collected from lactating animals. copro ... | 2008 | 18328629 |
| seroprevalence of neospora caninum antibodies in cattle and water buffaloes in india. | neospora caninum is now recognized as a major cause of abortion in cattle worldwide, but there is no report of n. caninum infection in cattle in india. serum samples from 427 dairy cattle and 32 dairy water buffaloes from 7 organized dairy farms located in punjab, india, were tested for n. caninum antibodies using a commercial monoclonal antibody-based competitive enzyme-linked immunosorbent assay (elisa). antibodies to n. caninum were found in 35 of 427 cattle from 6 of the 7 farms; 9.6% of cow ... | 2007 | 18314683 |
| bovine immune response to inoculation with neospora caninum surface antigen srs2 lipopeptides mimics immune response to infection with live parasites. | infection of cattle with neospora caninum protozoa, the causative agent of bovine protozoal abortion, results in robust cellular and humoral immune responses, particularly cd4(+) t-lymphocyte activation and gamma interferon (ifn-gamma) secretion. in the present study, n. caninum srs2 (ncsrs2) t-lymphocyte-epitope-bearing subunits were incorporated into dna and peptide preparations to assess cd4(+) cell proliferation and ifn-gamma t-lymphocyte-secretion immune responses in cattle with predetermin ... | 2008 | 18305105 |
| transplacental toxoplasmosis in a wild southern sea otter (enhydra lutris nereis). | in september 2004, a neonatal sea otter pup was found alive on the beach in northern monterey bay, ca. efforts to locate the mother were unsuccessful. due to a poor prognosis for successful rehabilitation, the pup was euthanized. postmortem examination revealed emaciation, systemic lymphadenopathy and a malformation of the left cerebral temporal lobe. on histopathology, free tachyzoites and tissue cysts compatible with toxoplasma gondii were observed in the brain, heart, thymus, liver, lymph nod ... | 2008 | 18304737 |
| seroprevalence of neospora caninum in dogs in south-western poland. | the prevalence of anti-neospora caninum antibodies was determined in serum samples from 110 dogs in south-western poland, using the indirect fluorescent antibody test (ifat). antibodies to n. caninum were found in 18 dogs (16.36%), of which 7 were also positive to toxoplasma gondii. the titres of anti-n. caninum antibodies varied from 1:50 to 1:200. | 2008 | 18291587 |
| high seroprevalence of neospora caninum in the red fox (vulpes vulpes) in the pyrenees (ne spain). | antibodies to neospora caninum were determined in the red fox (vulpes vulpes) in the pyrenees, north-eastern spain. sera from 53 red foxes (29 male and 24 female) were tested using a neospora agglutination test (nat). seroprevalence at dilutions of 1/40, 1/80 and 1/160 was 69.8%, 47.2% and 7.5%, respectively. sex differences were significant only at a dilution of 1:40. the high seroprevalence observed in red fox suggests that this species is highly exposed to n. caninum in this area. | 2008 | 18280664 |
| natural postnatal neospora caninum infection in cattle can persist and lead to endogenous transplacental infection. | a serological follow-up study of 3.5 years duration was done of a dairy herd that had experienced a mass seroconversion to neospora caninum following a point source exposure shortly before the 17th of january 2000. a total of 913 blood samples of 244 animals at seven sampling dates were used to investigate the seroprevalence dynamics in the herd. most postnatally infected cattle remained seropositive during the period of investigation but 11 animals became seronegative after 6-27 months indicati ... | 2008 | 18280662 |
| a survey of abortifacient infectious agents in livestock in luzon, the philippines, with emphasis on the situation in a cattle herd with abortion problems. | in the philippines, insufficient consideration has been given to the implementation of systematic control measures against major abortifacient infectious agents in livestock. to elucidate the epidemiology of abortifacient infectious agents in livestock, the prevalence of four abortifacient agents was assessed. initially, a total of 96 cattle including 17 cows with history of abortion were examined in a herd in luzon at the request of the farm owner. six (35.3%) of the 17 aborting cows were found ... | 2008 | 18243149 |
| seroepidemiology of neospora caninum and toxoplasma gondii infection in yaks (bos grunniens) in qinghai, china. | neospora caninum is a protozoan parasite and is closely related to toxoplasma gondii, but they are antigenically different. n. caninum and t. gondii infection in a variety of animals such as cattle, dogs, and cats has been reported, but there is little information on the infection of these parasites in domestic yaks. seroprevalence of antibodies to t. gondii and n. caninum in yaks (bos grunniens) from eight regions of qinghai, china were investigated by the indirect agglutination test (iat) and ... | 2008 | 18242860 |
| seroprevalence and risk factors associated with neospora caninum infection in different dog populations in spain. | in this study, neospora caninum seroprevalence and some associated risk factors were investigated in four different dog populations in spain. n. caninum seropositivity was significantly higher in farm dogs (51%, 51/100) (p<0.001) and lower in household dogs (2.9%, 3/102) (p<0.0001). the seroprevalence in hunting (23%, 23/100) and stray (24.5%, 23/94) dogs was moderate, and no significant differences were observed between these two populations (p>0.05). a significantly higher number of dogs showe ... | 2008 | 18241992 |
| serosurvey of ex situ giant pandas (ailuropoda melanoleuca) and red pandas (ailurus fulgens) in china with implications for species conservation. | conservation strategies for the giant panda (ailuropoda melanoleuca) include the development of a self-sustaining ex situ population. this study examined the potential significance of infectious pathogens in giant pandas ex situ. serologic antibody titers against canine distemper virus (cdv), canine parvovirus (cpv), canine adenovirus (cav), canine coronavirus (ccv), canine herpesvirus, canine parainfluenza virus (cpiv), toxoplasma gondii, neospora caninum, and leptospira interrogans were measur ... | 2007 | 18229861 |
| occurrence of toxoplasma gondii and hammondia hammondi oocysts in the faeces of cats from germany and other european countries. | faecal samples of 24,106 cats from germany and other european countries were examined microscopically in a veterinary laboratory in germany between october 2004 and november 2006 to estimate the prevalence of animals shedding toxoplasma gondii or hammondia hammondi oocysts. oocysts of 9-15 microm size with a morphology similar to that of h. hammondi and t. gondii were found in 74 samples (0.31%). a total of 54 samples were further characterised to achieve a species diagnosis and to determine the ... | 2008 | 18226453 |
| bayesian kriging of seroprevalence to mycobacterium avium subspecies paratuberculosis and neospora caninum in alberta beef and dairy cattle. | identifying spatial patterns of risk is important in the study of diseases with ecologic causes. furthermore, relatively complex hierarchical modeling is required to determine how factors that are organized across levels interact, such as how an ecologic cause interacts with farm management and with animal characteristics. the objective of this study was to map the risk for mycobacterium avium subspecies paratuberculosis (map - the causative agent of johne's disease) and neospora caninum (nc - t ... | 2007 | 18189052 |
| development of a rapid immunochromatographic test for simultaneous serodiagnosis of bovine babesioses caused by babesia bovis and babesia bigemina. | with the objective of developing a simpler diagnostic alternative, a rapid immunochromatographic test (boiict) was constructed for the simultaneous detection of babesia bovis- and babesia bigemina-specific antibodies using b. bovis recombinant merozoite surface antigen-2c and b. bigemina recombinant rhoptry-associated protein-1. the boiict selectively detected specific antibodies to b. bovis and b. bigemina. all sera from cattle infected with other protozoan parasites (i.e., cryptosporidium parv ... | 2008 | 18187794 |
| loss of infectivity of neospora caninum oocysts maintained for a prolonged time. | the purpose of this study was to investigate whether sporulated neospora caninum oocysts, which had been stored for 46 mo in a 2% sulfuric acid solution at 4 degrees c, remain morphologically viable and infective to gerbils (meriones unguiculatus). six gerbils were orally inoculated with doses of 400 or 1,200 oocysts. two mo after inoculation, the animals did not show any clinical signs, had no histological lesions, and were seronegative for n. caninum at 1: 50 in an immunofluorescent antibody t ... | 2007 | 18165712 |
| prevalence of neospora caninum and toxoplasma gondii antibodies in wild ruminants from the countryside or captivity in the czech republic. | in the czech republic, sera from 720 wild ruminants were examined for antibodies to neospora caninum by screening competitive-inhibition enzyme-linked immunosorbent assay and confirmed by indirect fluorescence antibody test (ifat); the same sera were also examined for antibodies to toxoplasma gondii by ifat. neospora caninum antibodies were found in 14% (11 positive/79 tested) roe deer (capreolus capreolus), 14% (2/14) sika deer (cervus nippon), 6% (24/ 377) red deer (cervus elaphus), 1% (2/143) ... | 2007 | 18163361 |
| vaccination with microneme protein ncmic4 increases mortality in mice inoculated with neospora caninum. | ncmic4 is a neospora caninum microneme protein that has been isolated and purified on the basis of its unique lactose-binding properties. we have shown that this protein binds to galactosyl residues of lactose; antibodies directed against ncmic4 inhibit host cell interactions in vitro, thus making it a vaccine candidate. because of this feature, ncmic4 was first purified on a larger scale in its native, functionally active form using lactose-agarose affinity chromatography. second, ncmic4 was ex ... | 2007 | 18163338 |
| neospora caninum and toxoplasma gondii antibodies in dogs from durango city, mexico. | toxoplasma gondii and neospora caninum are structurally similar parasites, with many hosts in common. the prevalence of antibodies to t. gondii and n. caninum was determined in sera from dogs from durango city, mexico. using a modified agglutination test, antibodies to t. gondii were found in 52 (51.5%) of the 101 dogs with titers of 1:25 in 27, 1:50 in 11, 1:100 in 5, 1:200 in 4, 1:400 in 2, 1:800 in 2, and 1:3,200 or higher in 1. antibodies to n. caninum were determined by the indirect immunof ... | 2007 | 18163336 |
| low seroprevalence of neospora caninum infection associated with the limousin breed in cow-calf herds in andorra, europe. | neospora caninum seroprevalence and risk factors affecting seroprevalence in beef cattle in andorra were investigated. antibodies to n. caninum were evaluated by enzyme-linked immunosorbent assay performed on a yearly basis in 1,758 animals older than 6 mo, belonging to 26 herds. mean seroprevalence of antibodies to n. caninum for the herds was 7.4 +/- 1.2% (130/1,758). logistic regression analyses were performed on data from each animal, considering n. caninum seropositivity as the dependent va ... | 2007 | 18163335 |
| studies on synchronous egress of coccidian parasites (neospora caninum, toxoplasma gondii, eimeria bovis) from bovine endothelial host cells mediated by calcium ionophore a23187. | neospora caninum, toxoplasma gondii and eimeria bovis are coccidian parasites of veterinary importance. tachyzoites of n. caninum and t. gondii and sporozoites of e. bovis are able to invade and replicate in endothelial cells in vivo and in vitro. as it holds true for all eukaryotic cells, the survival of parasitized host cells and the parasites themselves should be dependent on ion balances, especially on extra- and intracellular calcium concentrations. addition of the calcium ionophore a23187 ... | 2008 | 18158611 |
| neospora caninum infection in greek dairy cattle herds detected by two antibody assays in individual milk samples. | a survey to demonstrate the presence and the extent of neospora caninum infection in dairy cattle was carried out in greece. seven hundred and seventy-seven (777) individual milk samples from all milking cows in 10 dairy herds were tested using an elisa (p38-milk-elisa) and immunoblot analysis. the herd prevalence was 80.0% and 100.0%, while the overall prevalence was 15.2% (ranging between 0.0% and 38.9%) and 27.9% (ranging between 3.4% and 61.6%) based on the results of the elisa and the immun ... | 2008 | 18158217 |
| babesia gibsoni: serodiagnosis of infection in dogs by an enzyme-linked immunosorbent assay with recombinant bgtrap. | the thrombospondin-related adhesive protein of babesia gibsoni (bgtrap) is known as an immunodominant antigen and is, therefore, considered as a candidate for the development of a diagnostic reagent for canine babesiosis. the recombinant bgtrap (rbgtrap) expressed in escherichia coli was tested in an enzyme-linked immunosorbent assay (elisa) for detecting antibodies to b. gibsoni in dogs. the elisa with rbgtrap clearly differentiated between b. gibsoni-infected dog sera and specific pathogen-fre ... | 2008 | 18155197 |
| evaluation of an enzyme-linked immunosorbent assay for the serological diagnosis of neospora caninum infection in sheep and determination of the apparent prevalence of infection in new zealand. | neospora caninum has recently been shown to be a cause of abortions of sheep in new zealand. a commercially available enzyme-linked immunosorbent assay (elisa) was validated for use in sheep with sera from experimentally infected sheep. a cut-off threshold was established that demonstrated sero-conversion between 7 and 14 days post-infection. higher inocula led to earlier sero-conversion. this elisa was applied to 640 sera collected from rams across new zealand and 0.625% (+/-0.61%) (4/640) were ... | 2008 | 18083308 |
| prevalence of anti-neospora caninum antibodies in cattle from the state of mato grosso do sul, brazil. | neospora caninum is an obligate intracellular parasite that can infect domestic and wild canids, as well as ruminants and equines. it was described in 1988 and has been known as a major cause of abortion in bovines and neuromuscular alterations and death in dogs. to estimate the prevalence of bovine neosporosis in the 22 municipalities of the so-called estrato 1 subregion of the brazilian state of mato grosso do sul, blood samples were collected from cows aged 24 months and older, from december ... | 2007 | 18078599 |
| diagnosis of sarcocystis cruzi, neospora caninum, and toxoplasma gondii infections in cattle. | the aim of the study was to diagnose sarcocystis sp. infections in cattle and to detect coinfections by toxoplasma gondii and/or neospora caninum. blood, diaphragm, esophagus, and myocardium from 90 beef cattle from argentina were collected. histopathological, immunohistochemical, polymerase chain reaction assays, and direct microscopical examination were carried out. sarcocysts from myocardium were measured and counted. indirect fluorescent antibody test (ifat) for the three protozoans was perf ... | 2008 | 18066600 |
| chickens (gallus domesticus) are natural intermediate hosts of neospora caninum. | neospora caninum naturally infects many mammal species, but has not previously been demonstrated in birds. we examined sera for n. caninum antibodies from 200 outdoor chickens and from 200 chickens confined indoors in the state of bahia, brazil. seroprevalence was greater in outdoor chickens (23.5% versus 1.5%, p<0.001). pcr testing for n. caninum was positive in six of 10 seropositive chickens. amplicons from two of these were sequenced and had 97-98% nucleotide identity with n. caninum. this f ... | 2008 | 18054356 |
| sarcocystis neurona encephalitis in a dog. | a 1.5-year-old male feist dog was presented to a veterinarian for reluctance to stand on the hind legs. treatment included dexamethasone and resulted in a favorable initial response, but posterior paresis returned and progressed to recumbency, hyperesthesia, and attempts to bite the owner. the dog was euthanized. the brain was negative for rabies by fluorescent antibody analysis. multiple foci of encephalitis were found in the cerebrum and particularly in the cerebellum. protozoa morphologically ... | 2007 | 18039914 |
| anti-neosporal igg and ige antibodies in canine neosporosis. | neospora caninum infection provokes neurological disorders, recurrent abortion and death in dogs and cattle. dogs are both intermediate and definitive host of n. caninum. thus, the development of sensitive and specific immunoassays to diagnose canine neosporosis is essential to control this disease. this work investigated serum anti-neosporal igg and ige antibodies in 140 dogs represented by 30 healthy animals (group i), 11 dogs showing acute n. caninum infection (group ii), 50 urban dogs with s ... | 2007 | 18035978 |
| the extent of parasite-associated necrosis in the placenta and foetal tissues of cattle following neospora caninum infection in early and late gestation correlates with foetal death. | the protozoan parasite neospora caninum is the most frequently diagnosed abortifacient in the uk and a leading cause of abortion worldwide but the mechanisms leading to abortion are not fully understood. the distribution of parasites and the histopathological changes in the placenta and foetus were compared in 12 cows following experimental infection of cattle with n. caninum in early (n=6) and late (n=6) gestation, by pcr, immunohistology, light microscopy and transmission electron microscopy. ... | 2008 | 18021783 |
| c57bl/6 mice infected with neospora caninum during administration of progesterone show bias toward type 2 immune response. | to clarify the role of progesterone in the development of immune responses during pregnancy against neospora caninum infection, c57bl/6 mice were given a progesterone pellet, and measured on interferon-gamma and interleukin-4 production following the infection. ifn-gamma production in the prescribed group was significantly lower than that in the intact group on day 40 post administration. il-4 producing cell population in the prescribed group was larger than that in the intact group. these resul ... | 2007 | 17984602 |
| dense-granule protein ncgra7, a new marker for the serodiagnosis of neospora caninum infection in aborting cows. | to investigate whether the production of an antigen-specific antibody is associated with neospora caninum-induced bovine abortion, 62 serum samples were tested with an enzyme-linked immunosorbent assay using the recombinant antigens ncsag1, ncsrs2, and ncgra7. our study suggested that ncgra7 would be a new marker for the serodiagnosis of n. caninum infection resulting in abortion. | 2007 | 17959821 |
| the host-parasite relationship in pregnant cattle infected with neospora caninum. | the protozoan parasite neospora caninum is an important cause of reproductive disease in cattle worldwide. the dog is a definitive host for the parasite and the oocyst stage, shed in the faeces, is a source of infection for cattle through consumption of contaminated feed or water. in addition, transplacental transmission of n. caninum is a very efficient means of the parasite infecting a new host and this can occur in successive pregnancies and over several generations. neospora parasites may ca ... | 2007 | 17958926 |
| occurrence of antibodies against neospora caninum in water buffaloes (bubalus bubalis) on four ranches in corrientes province, argentina. | the aim of the present work was to describe the occurrence of antibodies to neospora caninum in water buffaloes on four ranches located in corrientes province in the northeast of argentina. antibodies against n. caninum were determined in sera of 449 water buffaloes by using an indirect fluorescent antibody test (ifat). a bayesian logistic regression mixed model was used to quantify the strength of association between positive serological results to n. caninum and gender, age and category (calf, ... | 2007 | 17950535 |
| in vitro analysis of the tat protein transduction domain as a drug delivery vehicle in protozoan parasites. | the present study was undertaken to analyse the capability of hiv-1 derived tat protein transduction domain (ptd) fused with green fluorescent protein (tat-gfp) as a delivery vehicle into a range of protozoan parasites. successful transduction of native purified tat-gfp was observed by fluorescent microscopy in cryptosporidium parvum, giardia duodenalis, and neospora caninum. the ability to transduce peptides and other cargo into protozoan parasites, will greatly assist in the delivery of future ... | 2008 | 17945214 |
| evaluation of neospora caninum and toxoplasma gondii infections in alpaca (vicugna pacos) and llama (lama glama) aborted foetuses from peru. | the aim of this study was to investigate the participation of neospora caninum and toxoplasma gondii in abortion cases of peruvian llamas and alpacas. fifteen aborted foetuses were recovered from two main rearing areas of camelids in peru (central or south andean region). foetal histopathology was used to detect the presence of protozoal-associated lesions in target organs. n. caninum and t. gondii infections were confirmed by immunohistochemistry (ihc) combined with pcr and by pcr alone, respec ... | 2007 | 17931783 |
| quantitative analysis of parasite dna in the blood of immunized and naïve mice after infection with neospora caninum. | real-time pcr was used to study the duration and level of parasitaemia in mice immunized with immune-stimulating complexes (iscoms) containing recombinant ncsrs2, one of the immunodominant surface antigens of neospora caninum. after challenge infection, blood was collected daily for 9 days. during this period the amounts of parasite dna detected in immunized mice were significantly lower (p<0.001), and the duration of parasitaemia appeared to be shorter, than in non-immunized controls. furthermo ... | 2008 | 17922929 |
| histopathological and immunohistochemical aspects of neospora caninum diagnosis in bovine aborted fetuses. | lesions suggestive of protozoal infection such as nonsuppurative myositis, nonsuppurative myocarditis, nonsuppurative pneumonia associated with focal cellular necrosis and multifocal necrotizing nonsuppurative encephalitis were observed in 89 (34%) out of 258 aborted bovine fetuses. immunohistochemistry (ihc) revealed tachyzoites and cysts of neospora caninum in 55 (61.8%) of these 89 fetuses. several fetal variables were tested by multivariate logistic regression, in which increasing odds for p ... | 2007 | 17904290 |
| epidemiology of neosporosis in dairy cattle in galicia (nw spain). | this comprehensive study of neosporosis in dairy cattle in galicia (nw spain) included: (1) a comparative study of three serological techniques for detection of neospora caninum antibodies (direct agglutination, enzyme-linked immunosorbent assay and indirect immunofluorescence); (2) a cross-sectional serological survey in which 276 herds and 5,196 animals were tested; (3) a study of n. caninum antibody dynamics; (4) the isolation of viable tachyzoites of n. caninum. data were analysed to determi ... | 2008 | 17899194 |
| neosporosis in beagle dogs: clinical signs, diagnosis, treatment, isolation and genetic characterization of neospora caninum. | clinical neosporosis was diagnosed in a litter of five pups born to a beagle bitch from virginia, usa. four of the pups developed limb weakness starting at 4 weeks of age. the dogs were suspected to have neosporosis based on clinical signs and empirically treated with clindamycin (75 mg, oral, twice daily, total 150 mg) starting at 9 weeks of age and the dosage was doubled at 13 weeks of age. antibodies to neospora caninum were detected in sera of the dam and pups when first tested serologically ... | 2007 | 17890012 |
| fecal and serological survey of neospora caninum in farm dogs in costa rica. | to detect oocysts of neospora caninum in dog feces and to determine the excretion pattern in dogs from specialized dairy farms in costa rica, a total of 265 fecal samples from 34 dogs were collected at intervals from february to august 2005. fecal samples were examined for n. caninum-like oocysts microscopically, by dna detection using the polymerase chain reaction (pcr), and by bioassay. n. caninum dna was detected by pcr in four fecal samples, twice from one dog, but oocysts were not detected ... | 2007 | 17868998 |
| protection against abortion linked to gamma interferon production in pregnant dairy cows naturally infected with neospora caninum. | many immunological aspects of pregnancy, such as the role played by gamma interferon (ifn-gamma) in abortion, are not well understood. neospora caninum is an intracellular protozoan considered to be among the main causes of abortion in cattle worldwide. the present study analyzes the interaction between ifn-gamma production and n. caninum infection in naturally infected pregnant cows. data were obtained from 126 pregnant cows: 86 seropositive and 40 seronegative for the parasite. pregnancy diagn ... | 2007 | 17854883 |