Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| exogenous nitric oxide triggers neospora caninum tachyzoite-to-bradyzoite stage conversion in murine epidermal keratinocyte cell cultures. | neospora caninum, like toxoplasma gondii, undergoes stage conversion in chronically infected animals, and forms tissue cysts which contain the slowly proliferating bradyzoite stage. these tissue cysts are delineated by a cyst wall, protect the parasite from physiological and immunological reactions on part of the host, and bradyzoites remain viable within an infected host for many years. however, unlike t. gondii, n. caninum bradyzoites have been difficult to obtain using in vitro culture techni ... | 2002 | 12204225 |
| serological investigation of aborted sheep and pigs for infection by neospora caninum. | serology for neospora caninum was undertaken using direct elisas on sera from 660 aborted sheep and 454 breeding sows, which had aborted or were considered infertile. all ovine sera were further tested by indirect fluorescent antibody test (ifat) for n. caninum, and a latex agglutination test (lat) for toxoplasma gondii was performed on 423 of the samples, including all those positive by elisa. elisa-positive porcine sera were tested by ifat and an inhibition elisa for antibodies to n. caninum a ... | 2002 | 12204640 |
| fetal infection with neospora caninum in dairy and beef cattle in belgium. | neospora caninum is a protozoan parasite, which causes fetal and neonatal mortality in livestock and companion animals. in 224 abortions in belgian cattle, different diagnostic methods were used to demonstrate infection, and the presence of n. caninum. an indirect fluorescent antibody test (ifat) was used to analyze fetal and maternal sera and immunohistochemistry (ihc) was performed when lesions consistent with neosporosis were observed in the brain, heart or liver. twenty dairy cattle sera out ... | 2002 | 12212893 |
| cutaneous neosporosis during treatment of pemphigus foliaceus in a dog. | a 4-year-old, intact male rottweiler was presented with a 10-day history of papulonodular dermatitis. at the time of presentation, the dog was receiving prednisone and azathioprine to treat pemphigus foliaceus. cutaneous neosporosis was diagnosed by immunohistochemistry on skin biopsy specimens and a high serum antibody titer to neospora caninum by neospora agglutination test. electron microscopy examination of skin specimens further supported the diagnosis. clindamycin therapy, together with wi ... | 2002 | 12220024 |
| confirmation of the prevention of vertical transmission of neospora caninum in cattle by the use of embryo transfer. | 2002 | 12224621 | |
| management factors related to seroprevalences to bovine viral-diarrhoea virus, bovine-leukosis virus, mycobacterium avium subspecies paratuberculosis, and neospora caninum in dairy herds in the canadian maritimes. | bovine viral-diarrhoea (bvd), enzootic bovine leukosis (ebl), johne's disease (jd), and neosporosis lower on-farm productivity, reduce export competitiveness, and increase consumer concerns regarding safety. our purpose was to examine the relationship between 27 control practices and the estimated true seroprevalences for these four diseases for 2604 cattle in 90 dairy herds in the maritimes provinces of canada. overall, 37.8, 20.4, 3.4, and 19.2% of all sampled cattle were truly exposed to the ... | 2002 | 12324207 |
| direct production losses and treatment costs from bovine viral diarrhoea virus, bovine leukosis virus, mycobacterium avium subspecies paratuberculosis, and neospora caninum. | our purpose was to determine direct production losses (milk loss, premature voluntary culling and reduced slaughter value, mortaliy loss, and abortion and reproductive loss) and treatmetn costs (veterinary services, medication cost, and extra farm labour cost) due to four infectious diseases in the maritime provinces of canada: bovine viral diarrhoea (bvd), enzootic bovine leukosis (ebl), johne's disease (jd), and neosporosis. we used a partial-budget model, and incorporated risk and sensitivity ... | 2002 | 12350317 |
| neospora caninum: a cause of immune-mediated failure of pregnancy? | resistance to many intracellular protozoan parasites is dependent on t helper cell 1 cytokine responses. this has important repercussions for pregnant females because strong t helper cell 1 cytokine responses are incompatible with successful pregnancy. thus, there are two possible consequences of infection with protozoans such as leishmania major, plasmodium falciparum and toxoplasma gondii during pregnancy: (1) pregnancy is compromised; or (2) resistance to the parasite is compromised. the apic ... | 2002 | 12377255 |
| neospora caninum: oocyst challenge of pregnant cows. | three pregnant cows were each orally challenged at 10 weeks of gestation with 600 sporulated oocysts of neospora caninum. the number of oocysts was limited by those available. in concurrent bioassays, one oocyst per os infected each of two gerbils. challenged cattle developed neospora-specific antibody, cell proliferation and gamma-interferon responses. n. caninum specific pcr demonstrated persisting infection in the brains of cows 4 months after calving. abortion was not induced and there was n ... | 2002 | 12383634 |
| neospora caninum serostatus and milk production of holstein cattle. | to determine whether neospora caninum serostatus was associated with milk production among holstein cattle in ontario. | 2002 | 12387387 |
| neospora caninum serostatus and culling of holstein cattle. | to determine whether time until culling or risk of culling was associated with neospora caninum serostatus among holstein cattle in dairy herds in ontario. | 2002 | 12387388 |
| steroid hormones do not reactivate neospora caninum in ovariectomized mice. | the direct effects of three steroid hormones (progesterone, estradiol-17beta and corticosterone) on the growth of neospora caninum (n. caninum) tachyzoite were examined in vero cells. subsequently, ovariectomized balb/c mice infected with n. caninum were treated with physiological concentrations of the steroid hormones for 1 or 2 weeks. these hormones had no direct effect on the parasite growth in vitro. in the infected mice, there was no significant difference in the parasite distribution and h ... | 2002 | 12399600 |
| validation of 2 commercial neospora caninum antibody enzyme linked immunosorbent assays. | this is a validation study of 2 commercially available enzyme linked immunosorbent assays (elisa) for the detection of antibodies against neospora caninum in bovine serum. the results of the reference sera (n = 30) and field sera from an infected beef herd (n = 150) were tested by both elisas and the results were compared statistically. when the immunoblotting results of the reference bovine sera were compared to the elisa results, the same identity score (96.67%) and kappa values (k) (0.93) wer ... | 2002 | 12418782 |
| prevalence of antibodies to neospora caninum in korean native beef cattle. | a total of 438 sera from korean native beef cattle in 9 provinces were tested for neospora caninum antibodies using an immunofluorescent antibody test (ifat). eighteen (4.1%) cattle were positive by ifat. the titers ranged from 1:200 (10 animals), 1:400 (5 animals), 1:800 (2 animals) to 1:1,600 (1 animal). although the seroprevalence was slightly higher in chungnam (8.9%), this was not significantly different from those noted in kyunggi, kangwon, kyungbuk, kyungnam, and cheju provinces. sera obt ... | 2002 | 12419873 |
| dog shedding oocysts of neospora caninum: pcr diagnosis and molecular phylogenetic approach. | results of molecular determination of a dog isolate of neospora caninum in the czech republic are presented. colorless bisporocystic oocysts measuring 10-13 micro m x 10-11 micro m were recovered from feces and used for dna isolation. a diagnostic pcr procedure using previously described molecular methods was performed to determine the species. the n. caninum species-specific primers based on the nc 5 region produced a positive result, while primers specific for hammondia heydorni rdna internal ... | 2002 | 12423929 |
| neospora caninum ncsrs2 is a transmembrane protein that contains a glycosylphosphatidylinositol anchor in insect cells. | we investigated the terminal location of ncsrs2, a surface antigen of neospora caninum that has potential use for diagnosis, and demonstrated its importance as a vaccine component against neosporosis, in an insect-baculovirus expression system. to examine the role of the hydrophobic c-terminal tail in ncsrs2, four types of recombinant baculoviruses were constructed. immunoblotting and n-terminal amino acid analysis revealed cleavage of a 6 kda of the n-terminal signal peptide in the mature ncsrs ... | 2002 | 12423932 |
| a high rate of seroconversion for neospora caninum in a dairy herd without an obvious increased incidence of abortions. | the purpose of this paper is to investigate the high rate of seroconversion for neospora caninum in a dairy herd. forty-five of 95 (47%) of the seronegative animals seroconverted within a period of 6 months. seropositive animals were not equally distributed among age-groups. a high seroprevalence age-group of 8-30 months was housed together during a period of 4 months, indicating a point source infection within this period. a lack of association between the serological status of daughters and mo ... | 2002 | 12423933 |
| an explorative study to assess the efficacy of toltrazuril-sulfone (ponazuril) in calves experimentally infected with neospora caninum. | neospora caninum is an important cause of infectious abortion and stillbirth in cattle world-wide. infection is common and may frequently be passed from mother to calf (vertical transmission) with no signs of disease. based on our previous observation that n. caninum-infection can be efficiently controlled with toltrazuril-sulfone (ponazuril) in experimentally infected mice, we addressed the question if efficacy could also be obtained in experimentally infected calves. | 2002 | 12437777 |
| first portuguese isolate of neospora caninum from an aborted fetus from a dairy herd with endemic neosporosis. | neospora caninum was isolated from the brain of an aborted 4-month-old fetus from a dairy cow herd with endemic neosporosis in porto, portugal. the fetal brain homogenate was inoculated interperitoneally first into outbred swiss webster mice given dexamethasone and then the peritoneal exudates from these mice was co-inoculated with mouse sarcoma cells in the peritoneal cavity of mice given dexamethasone. n. caninum tachyzoites were seen in peritoneal exudate of the second passage. tachyzoites fr ... | 2002 | 12446085 |
| seroprevalence of neospora caninum infection in dairy and beef cattle in paraguay. | seroprevalence of neospora caninum in 879 beef and dairy cattle in different locations of paraguay was determined by an elisa. in the survey, 262 (29.8%) cattle were positive to n. caninum, and animals with anti-neospora antibody titre were observed in all the locations tested in the country. serum samples taken from a herd that exhibited persistent abortion had the highest percentage of animals being positive to the parasite (17/30, 56.7%). in the same herd, abortion was significantly more like ... | 2002 | 12446086 |
| use of bulk milk for detection of neospora caninum infection in dairy herds in thailand. | the relationship between the level of neospora caninum antibodies in bulk milk and the seroprevalence in lactating cows was investigated. bulk milk was also used to estimate the prevalence of n. caninum infection in dairy herds in the northeast and north thailand. bulk milk and individual serum from all lactating cows in 11 herds as well as 220 bulk milk samples from nine milk collection centres were analysed for presence of n. caninum antibodies using an iscom elisa. in the 11 herds the bulk mi ... | 2002 | 12446088 |
| transplacental toxoplasmosis in a reindeer (rangifer tarandus) fetus. | toxoplasma gondii infection was diagnosed in a full term stillborn reindeer (rangifer tarandus) fetus. the fetus had encephalitis and placentitis associated with t. gondii. tissue cysts were identified histologically in sections of brain and tachyzoites were present in placenta and the myocardium. protozoa in the brain, heart, and placenta stained positively with t. gondii antibodies, but not with neospora caninum antibodies in an immunohistochemical test. the dam of the fetus had a 1:12,800 tit ... | 2002 | 12446098 |
| characterisation of the first australian isolate of neospora caninum from cattle. | to isolate neospora caninum from a congenitally infected calf. | 2002 | 12465814 |
| immune responses to neospora caninum and prospects for vaccination. | developing an effective vaccine against neosporosis presents several interesting challenges. the parasite is spread efficiently from mother to foetus over several generations, and naturally infected cattle do not appear to develop adequate protective immunity. modulation of the immune response during pregnancy favours parasite survival and multiplication. however, induction of pro-inflammatory responses that are thought to be protective against neospora caninum would be detrimental to the pregna ... | 2002 | 12473366 |
| the comparative pathogenesis of neosporosis. | since its first description in dogs in 1984, the protozoan parasite neospora caninum has been found to infect a wide range of animals, worldwide. in cattle, n. caninum has particular significance as a cause of abortion in which persistence of infection in the mother, recrudescence of the parasite during pregnancy, and the vulnerability of the placenta and foetus to invasion are important features. this article discusses how neospora invades cells, how it infects and causes disease in several ani ... | 2002 | 12482540 |
| [abortion in small ruminants in switzerland: investigations during two lambing seasons (1996-1998) with special regard to chlamydial abortions]. | abortion cases of 144 goats und 86 sheep were investigated etiologically during 2 lambing seasons (1996/1997, 1997/1998). macroscopic inspection of fetus and placenta was completed by histopathology and bacteriological isolation of agents. in addition, immunohistologically the following antigens were labeled in formalin-fixed and paraffin-embedded tissue sections: toxoplasma gondii, neospora caninum, chlamydophila abortus (formerly chlamydia psittaci serovar 1) and border disease virus. from far ... | 2002 | 12677687 |
| neospora caninum in vitro: evidence that the destiny of a parasitophorous vacuole depends on the phenotype of the progenitor zoite. | we previously reported that neospora caninum can be induced to express bagi, a bradyzoite antigen, within 3 days of culture under stress conditions. the main goals of the present experiment were to increase the expression of bagi in vitro (in part by extending cultures for 9 days), to observe parasitophorous vacuoles at various points of stage differentiation, and to test the ability of organisms produced in vitro to function like mature bradyzoites. expression of bag1 and of a tachyzoite antige ... | 2002 | 12537100 |
| characterization of neospora caninum protease, ncsub1 (nc-p65), with rabbit anti-n54. | ncsub1 (formerly known as nc-p65) is the first molecularly described proteolytic enzyme of the intracellular protozoan parasite neospora caninum. this report describes the characterization of a rabbit anti-n54, which is an antiserum generated against an internal fragment of ncsub1 (amino acids 649-783). in immunofluorescence studies rabbit and-n54 labeled the apical end of the fixed parasite. by immuno-gold electron microscopy, the antibody bound primarily to the microneme organelles of the para ... | 2002 | 12537103 |
| experimental infection of sheep with neospora caninum oocysts. | the purpose of the present study was to investigate the potential of neospora caninum oocysts to infect sheep and determine whether n. caninum dna could be detected by polymerase chain reaction (pcr) assay in blood and brain of sheep after oocyst inoculation. six ewes were inoculated per os with 10(4) n. caninum oocysts, whereas 2 ewes served as uninoculated controls. all sheep were bled weekly for 7 wk after inoculation. blood was analyzed for the presence of n. caninum dna by 2 different pcr a ... | 2002 | 12537104 |
| improved production of neospora caninum oocysts, cyclical oral transmission between dogs and cattle, and in vitro isolation from oocysts. | scarce information is available about neospora caninum oocysts and sporozoites, in part because only small numbers of oocysts have typically been produced by experimentally infected dogs. we hypothesized that i reason for low experimental production of oocysts is that dogs have been fed tissues from experimentally infected mice instead of tissues from cattle (which are natural intermediate hosts of n. caninum). in this study, 9 dogs were fed tissues from n. caninum-infected calves, and oocyst pr ... | 2002 | 12537111 |
| isolation of viable toxoplasma gondii from naturally infected aborted bovine fetuses. | neospora caninum and toxoplasma gondii are related parasites. the former is a common cause of abortion in dairy cattle. the latter has not been conclusively demonstrated in bovine fetuses. during the course of attempts to isolate n. caninum from aborted fetuses, t. gondii was isolated from 2 aborted fetuses, 1 from portugal and 1 from the united states. both isolates were made by bioassay of fetal brains in mice. the fetus from portugal was about 5 mo in gestational age, and the fetus from the u ... | 2002 | 12537120 |
| prevalence of antibodies to neospora caninum and sarcocystis neurona in sera of domestic cats from brazil. | parasite biology, epidemiology and systematics laboratory, animal and natural resources institute, agricultural research service, united states department of agriculture, building 1001, beltsville, maryland 20705-2350 antibodies to neospora caninum and sarcocystis neurona were determined in serum samples of 502 domestic cats from brazil using direct agglutination tests with the respective antigens. antibodies to s. neurona were not found in 1:50 dilution of any serum in the s. neurona agglutinat ... | 2002 | 12537122 |
| seroprevalence of antibodies to neospora caninum in dogs from spain. | the prevalence of neospora caninum antibodies was determined in sera of 139 dogs from catalonia (northeastern spain) using the indirect immunofluorescence antibody test (ifat). antibodies in the ifat were found in 17 of 139 dogs (12.2%) with titers ranging from 1:50 to 1: 1,600. seroprevalence was higher in dogs over 1 yr old compared with dogs younger than 1 yr (p < 0.05). no statistical difference was observed when sex, breed, purpose, or modus vivendi was compared with seropositivity. most do ... | 2002 | 12537126 |
| survey of neospora caninum antibodies in dairy and beef cattle from five regions of the united states. | serum samples from 4,907 cows from 93 dairy and five beef herds were analyzed for antibodies to neospora caninum by commercially available elisa. the herds were recruited from 20 states and the territory of puerto rico. a questionnaire assessed the herd's abortion history and exposure risks to n. caninum. results showed that 788 (16%) cows tested positive for antibodies to n. caninum. at least one positive animal was detected in 90% of the herds tested. the prevalence of cows seropositive to n. ... | 2002 | 12584676 |
| control options for neospora caninum infections in cattle--current state of knowledge. | this article reviews control options for neospora caninum infection and abortion in cattle, drawing on published literature and the authors' own research in this field. apart from the successful use of embryo transfer to prevent congenital infection in calves born to infected cows, there are currently no accepted control methods for the prevention of abortions in cattle. the epidemiological data at hand suggest that concomitant infections with bovine pestivirus increase the risk of abortion sign ... | 2002 | 16032217 |
| recent advances in understanding the epidemiology of neospora caninum in cattle. | introduction: neospora caninum is the most frequently diagnosed cause of cattle abortion in new zealand and is an important pathogen worldwide. the parasite has been found in aborted bovine foetuses and in puppies with neurological disease. recent developments have provided new insights into the epidemiology of the disease that are reviewed in this article. transmission: transplacental infection is of major importance in the spread of n. caninum overseas. most congenital infections result in the ... | 2001 | 16032161 |
| efficacy of toltrazuril and ponazuril against experimental neospora caninum infection in mice. | neosporosis is a disease affecting predominantly fetal development in cattle and dog hosts; and it may cause neuromuscular disfunction in infected newborn calves and pups. predispositions--including, e.g. transient immunosuppression during pregnancy--may result in an increased dissemination of the parasite within the host or its offspring. chemotherapeutic treatment of neosporosis may be an issue, provided that an appropriate drug is made available. in this respect, we describe the use of a mous ... | 2001 | 11199848 |
| prevalence of neospora hughesi and sarcocystis neurona antibodies in horses from various geographical locations. | parasite-specific antibody responses to neospora antigens were detected using the immunofluorescent antibody test (ifat) and immunoblot analysis in select equine populations. for comparison, a naturally infected neospora hughesi horse and an experimentally inoculated neospora caninum horse were used. in addition, all samples were tested for antibodies to sarcocystis neurona by immunoblot analysis. a total of 208 samples was evaluated. the equine populations were derived from five distinct geogra ... | 2001 | 11223207 |
| molecular comparison of the dense granule proteins gra6 and gra7 of neospora hughesi and neospora caninum. | neospora hughesi is a recently described apicomplexan parasite that has been associated with several cases of equine protozoal myeloencephalitis. the biology of this new parasite is just beginning to be defined. towards this understanding, we report important differences between the nucleotide and deduced amino acid sequences of the dense granule proteins gra6 and gra7 of n. hughesi and neospora caninum. this information can be used to differentiate the two species and contribute to further unde ... | 2001 | 11226451 |
| evidence of post-natal transmission of neospora caninum in dutch dairy herds. | eighteen dairy herds with neosporosis-associated abortions were analysed for antibodies against neospora caninum. blood samples of all cows, heifers and calves were collected on the same day for each farm. a total of 2430 heads of cattle were examined. for each herd, the seropositive and seronegative animals were plotted against month of birth. analysis of seroprevalence in relation to age showed an equal distribution of seropositives in all age-groups in 10 herds. in contrast, in eight herds an ... | 2001 | 11239942 |
| immunity to experimental neosporosis in pregnant sheep. | neospora caninum is an important cause of fetal loss in cattle but has also infrequently been shown to cause disease in sheep and goats. experimental infection of pregnant sheep with n. caninum causes clinical and pathological changes very similar to those of neosporosis in cattle. an experiment in sheep was undertaken to examine whether infection with n. caninum before pregnancy conferred immunity to subsequent challenge with the parasite during pregnancy. primary inoculation of nc1 tachyzoites ... | 2001 | 11240899 |
| a previous infection with toxoplasma gondii does not protect against a challenge with neospora caninum in pregnant sheep. | sheep immunized with toxoplasma gondii (toxovax) prior to pregnancy were tested for their ability to withstand a challenge at 90 days gestation with 107 neospora caninum (nc1) tachyzoites. the antibody responses in sheep following immunization with t. gondii were specific for t. gondii whereas peripheral blood mononuclear cells responded to both t. gondii and n. caninum antigen in vitro. this suggested that there was induction of crossreactive immune recognition in the sheep, at least at the cel ... | 2001 | 11240903 |
| prevalence of antibodies to neospora caninum in horses in france. | 2001 | 11266072 | |
| seroprevalence of neospora caninum in beef cattle in northern alberta. | blood samples were collected from 1806 pregnancy-tested cows from 174 herds at a northern alberta auction mart in the fall of 1998. one hundred sixty-two (9.0%) of these samples were positive for antibodies to n. caninum. thirty-five of 260 samples (13.5%) collected from the same region in the 1980s were also serologically positive for n. caninum. | 2001 | 11272457 |
| toxoplasma gondii: molecular cloning and characterization of a novel 18-kda secretory antigen, tgmic10. | hoff, e. f., cook, s. h., sherman, g. d., harper, j. m., ferguson, d. j. p., dubremetz, j. f., and carruthers, v. b. 2001. toxoplasma gondii: molecular cloning and characterization of a novel 18-kda secretory antigen, tgmic10. experimental parasitology, 97, 77-88. during host cell invasion, toxoplasma gondii secretes proteins from specialized organelles (micronemes and rhoptries) located at the apical end of the parasite. the contents of the micronemes appear to be crucial to t. gondii invasion, ... | 2001 | 11281704 |
| rare detection of neospora caninum in placentas from seropositive dams giving birth to full-term calves. | neospora caninum is thought to be transmitted to cattle by dogs, the only known definitive host. although aborted fetuses seem the most likely source of infective material for dogs, placentas from seropositive dams appear also as a potential source of infective material. the objective of the study was to evaluate the presence of n. caninum organisms in placentas of full-term calves born to seropositive cows. sixteen placentas, 11 from neospora-seropositive cows, were examined histologically and ... | 2001 | 11289218 |
| a stochastic model for disease transmission in a managed herd, motivated by neospora caninum amongst dairy cattle. | a stochastic model for the spread of neospora caninum infection within a herd of dairy cattle is studied, in particular the long-term (equilibrium) behaviour of the model. the model incorporates the interesting feature that total herd size is constrained to lie within a fairly small interval, but not held exactly constant. approximations for the joint distribution of numbers of susceptible and infected individuals present in equilibrium are derived based upon a diffusion approximation to the inf ... | 2001 | 11292494 |
| serological evidence for naturally occurring transmission of neospora caninum among foxes (vulpes vulpes). | the study describes the time course of the neospora caninum-specific antibody response in experimentally infected foxes, in naturally n. caninum-seropositive vixens and their litters. an immunofluorescence test, a tachyzoite surface antigen based elisa and an immunoblot assay were established for this purpose. the immunoblot patterns of naturally seropositive and experimentally infected foxes revealed a high degree of similarity and resembled those reported for other intermediate host species. r ... | 2001 | 11306121 |
| characterization of the oregon isolate of neospora hughesi from a horse. | neospora hughesi was isolated in cell cultures inoculated with homogenate of spinal cord from a horse in oregon. tachyzoites of this oregon isolate of n. hughesi were maintained continuously by cell culture passage and tachyzoites were infective to immunosuppressed mice. gamma interferon gene knockout (ko) mice injected with tachyzoites developed fatal myocarditis and numerous tachyzoites were seen in lesions. gerbils (meriones unguiculatus) inoculated with tachyzoites developed antibodies (> or ... | 2001 | 11318565 |
| specific detection of neospora caninum oocysts in fecal samples from experimentally-infected dogs using the polymerase chain reaction. | neospora caninum oocysts, passed in the feces of a definitive host (dog), were isolated, and genomic dna was extracted. a polymerase cahin reaction (pcr) targeting the n. caninum-specific nc 5 genomic sequence was performed using the isolated dna. a synthesized competitor molecule containing part of the nc 5 sequence was included in the assay as a check against false-negative pcr results and to quantify n. caninum oocyst dna in fecal samples. a standard curve of the ratio of fluorescence intensi ... | 2001 | 11318571 |
| myosin diversity in apicomplexa. | a polymerase chain reaction (pcr) screen was used to examine the diversity of myosins in 7 apicomplexan parasites: toxoplasma gondii, plasmodium falciparum, neospora caninum, eimeria tenella, sarcocystis muris, babesia bovis, and cryptosporidium parvum. using degenerate pcr primers compatible with the majority of known myosin classes, putative myosin sequences were obtained from all of these species. all of the sequences obtained showed greatest similarity to previously identified apicomplexan m ... | 2001 | 11318578 |
| naturally-occurring neospora caninum infection in an adult sheep and her twin fetuses. | neospora caninum tissue cysts were found in the brains of surgically delivered twin fetuses at 119 days of gestation. in the brains of both fetuses, there was an inflammatory reaction involving perivascular cuffings of mononuclear cells, glial nodules. the dam of these fetuses died because of metritis. histopathological examination of the ewe revealed n. caninum tissue cysts and focal gliosis with mononuclear cell cuffings. a n. caninum-specific dna fragment was detected in a brain homogenate of ... | 2001 | 11318580 |
| comparison of the growth inhibitory effects of canine ifn-alpha, -beta and -gamma on canine cells infected with neospora caninum tachyzoites. | the growth inhibitory effects of recombinant canine interferon alpha (ifn-alpha), beta (ifn-beta) and gamma (ifn-gamma) were examined on madin-darby canine kidney cells infected with neospora caninum tachyzoites. the parasite growth was inhibited by all ifns in a dose-dependent manner. ifn-gamma inhibited the parasite growth with greater efficacy than ifn-alpha or ifn-beta. moreover, the effect of ifns on n. caninum growth associated with the suppression of the host cell viability. the present s ... | 2001 | 11346181 |
| experimental studies on the transmission of neospora caninum between cattle. | three studies were conducted to investigate the transmission of neospora caninum between cattle by the oral route. in the first study, six calves were dosed with 10(7)n caninum tachyzoites (nc livb1) in colostrum and/or milk replacer on four occasions. in the second study, two calves and two cows were fed placental tissues from n caninum -infected cows, and, in the third study, seven uninfected calves were fostered onto n caninum -infected dams. in the first study, all six calves developed antib ... | 2001 | 11356096 |
| failure of dogs to shed oocysts after being fed bovine fetuses naturally infected by neospora caninum. | neospora caninum is a protozoan that causes abortion in cattle. the dog has recently been identified as a definitive host for n. caninum. to verify if bovine fetuses can infect dogs, nine 2-4-month-old dogs were fed bovine fetuses naturally infected by n. caninum. none of the dogs excreted oocysts, seroconverted, had clinical signs or lesions compatible with n. caninum infection. additional studies will be necessary to determine the natural mode of infection of dogs by n. caninum. | 2001 | 11358630 |
| neospora caninum infection associated with stillbirths in captive antelopes (tragelaphus imberbis). | neosporosis was diagnosed in full-term stillborn twin calves of captive antelopes (tragelaphus imberbis) in a german zoo. in both calves a multifocal non-suppurative encephalitis was present and infection with neospora (n.) caninum was confirmed by foetal serology and pcr. n. caninum-dna was detected in brain, heart, lung, liver and spleen of a third full-term stillborn calf. histopathological lesions were absent in this calf and antibodies against n. caninum were not detected in pleural fluid. ... | 2001 | 11358631 |
| prevalence of antibodies to neospora caninum and toxoplasma gondii in gray foxes (urocyon cinereoargenteus) from south carolina. | little is known about the epidemiology of neospora caninum in wild mammal populations. it has been suggested that a sylvatic cycle exists for n. caninum. dogs and potentially other canids are a definitive host for n. caninum. the present study was done to determine the prevalence of antibodies to n. caninum in a population of gray foxes (urocyon cinereoargenteus) from a nonagricultural setting in south carolina. we also determined the prevalence of antibodies to toxoplasma gondii in these animal ... | 2001 | 11358632 |
| reproductive performance of a cow-calf herd following a neospora caninum-associated abortion epidemic. | this study examines the long-term impact of a neospora caninum-associated abortion outbreak in a large cow-calf herd in northern alberta. blood samples were collected 4 times from all bred females and heifer calves born during the spring before the outbreak: (1) at the time of the outbreak, (2) the following spring, (3) the subsequent fall, and, finally, (4) the second spring after the outbreak. the samples were analyzed using a commercially available enzyme-linked immunosorbent assay for n. can ... | 2001 | 11360856 |
| evaluation of the embryo transfer procedure proposed by the international embryo transfer society as a method of controlling vertical transmission of neospora caninum in cattle. | to evaluate efficacy of embryo transfer into seronegative recipients, using the procedure proposed by the international embryo transfer society (iets), for preventing vertical transmission of neospora caninum in cattle. | 2001 | 11394835 |
| bovine abortion associated with neospora caninum in hungary. | the authors report the first case of bovine neosporosis in hungary based on investigations made on an aborted fetus. the diagnostic methods included traditional as well as molecular techniques. this record extends further the geographic range of the disease. | 2001 | 11402646 |
| dogs shed neospora caninum oocysts after ingestion of naturally infected bovine placenta but not after ingestion of colostrum spiked with neospora caninum tachyzoites. | an experiment was carried out to determine whether bovine colostrum or placenta could be a source of infection of neospora caninum for dogs. for this purpose, two dogs were fed bovine colostrum to which culture-derived n. caninum tachyzoites were added and two other dogs were fed placental cotyledonary tissue from n. caninum seropositive cows. one dog served as a negative control during the start of the experiment but this control dog was fed cotyledonary tissue later on. none of the dogs did pr ... | 2001 | 11403764 |
| detection of igg antibodies to neospora caninum and toxoplasma gondii in dogs examined in a veterinary hospital from brazil. | a total of 163 dogs with neuromuscular, respiratory and/or gastrointestinal disorders, was admitted at the veterinary hospital, federal university of uberlândia, brazil, and submitted to serology for toxoplasma gondii and neospora caninum. assays for t. gondii included indirect haemagglutination (iha), indirect fluorescent antibody (ifat-tg), immunoenzymatic (elisa), and immunoblotting (ib-tg). assays for n. caninum included ifat-nc and immunoprecipitation (ip-nc). based on concordant results by ... | 2001 | 11423182 |
| first isolation of neospora caninum from the feces of a naturally infected dog. | neospora caninum is a major cause of abortion in cattle worldwide. cattle become infected with n. caninum by ingesting oocysts from the environment or transplacentally from dam to fetus. experimentally, dogs can act as definitive hosts, but dogs excrete few oocysts after ingesting tissue cysts. a natural definitive host was unknown until now. in the present study, n. caninum was isolated from the feces of a dog. gerbils (meriones unguiculatus) fed feces from the dog developed antibodies to n. ca ... | 2001 | 11426726 |
| in the absence of endogenous gamma interferon, mice acutely infected with neospora caninum succumb to a lethal immune response characterized by inactivation of peritoneal macrophages. | following infection with neospora caninum, balb/c mice were shown to be resistant to an acute infection but developed a latent chronic infection. however, balb/c background gamma interferon (ifn-gamma)-deficient mice were sensitive to the acute infection. since the immune response in ifn-gamma-deficient mice is scantly known, we examined the function of macrophages, major histocompatibility complex (mhc) class ii expression, t-cell responses, and serum cytokine levels in the mice. all ifn-gamma- ... | 2001 | 11427432 |
| immunohistochemical and ultrastructural evidence for neospora caninum tissue cysts in skeletal muscles of naturally infected dogs and cattle. | protozoan stages were detected in the skeletal muscles of four dogs suffering from neosporosis and two neonatal calves with confirmed neospora caninum- infection which could be immunohistochemically labelled by an antiserum against the bradyzoite-specific antigen bag-5. in one calf, a tissue cyst was labelled by an antiserum against the n. caninum isolate nc-1. ultrastructurally, a 0.3-1 microm-thick cyst wall surrounded the labelled parasites. the cysts were located within myofibres and contain ... | 2001 | 11429181 |
| neosporosis in mexican dairy herds: lesions and immunohistochemical detection of neospora caninum in fetuses. | of 211 aborted bovine fetuses collected from mexican dairy herds between january 1996 and march 1999, 73 showed microscopical lesions consistent with neosporosis. of these 73 fetuses, 58 (79%) showed lymphocytic myocarditis, 39 (53%) showed microgliosis and multifocal necrosis in the brain, 39 (53%) showed lymphocytic hepatitis, and 19 (26%) showed lymphocytic myositis. immunohistochemical examination of brain, myocardium and liver from 53 of the same 73 fetuses demonstrated neospora caninum ant ... | 2001 | 11437517 |
| prevalence of neospora caninum antibodies in wild ruminants from the italian alps. | 2001 | 11442237 | |
| genetic and biological diversity among isolates of neospora caninum. | neospora caninum is a protozoan parasite that causes bovine abortion. the epidemiology of n. caninum is poorly understood and little is known about the genetic diversity of the parasite, or whether individual isolates differ in virulence. such diversity may, among other factors, underlie the range of pathologies seen in cattle. in this study we analysed biological and genetic variation in 6 isolates of n. caninum originating from canine and bovine hosts by measurement of growth rate in vitro, we ... | 2001 | 11467779 |
| interferon-gamma-induced apoptosis in host cells infected with neospora caninum. | interferon-gamma (ifn-gamma) has a crucial role for host defence against parasite infection. it is not clear, however, how ifn-gamma affects the parasite-infected host cells. the effect of ifn-gamma on neospora caninum-infected cells was investigated in murine fibroblasts and canine kidney cells in vitro. in the presence of ifn-gamma, the viability of the infected host cell was decreased and apoptotic cell death occurred, as analysed by dna stainings with propidium iodide and a terminal deoxy-nu ... | 2001 | 11467780 |
| cutaneous neosporosis in two adult dogs on chronic immunosuppressive therapy. | antemortem diagnosis of generalized ulcerative and pyogranulomatous dermatitis with numerous intralesional tachyzoites was made from skin biopsy specimens from 2 adult dogs on chronic immunosuppressive therapy. a 9-year-old italian greyhound was on long-term corticosteroid therapy for the treatment of a lupus-like systemic autoimmune disorder, and a 7-year-old labrador retriever had received several months of chemotherapy for lymphosarcoma. the tachyzoites were identified as neospora caninum by ... | 2001 | 11482605 |
| seroprevalence of neospora caninum in female water buffaloes (bubalus bubalis) from the southeastern region of brazil. | antibodies to neospora caninum were assayed in sera of 222 female water buffaloes from ribeira valley of são paulo state, brazil, using an indirect fluorescent antibody test (ifat) and neospora agglutination test (nat). ifat antibodies were found in 64% of buffaloes with titers of 1:25 (42 buffaloes), 1:50 (53 buffaloes), 1:100 (31 buffaloes), 1:200 (10 buffaloes), 1:400 (3 buffaloes), or > or =1:800 (3 buffaloes). nat antibodies were found in 53% of buffaloes; in titers of 1:40 in 52 buffaloes, ... | 2001 | 11511420 |
| tgm2ap participates in toxoplasma gondii invasion of host cells and is tightly associated with the adhesive protein tgmic2. | like other members of the medically important phylum apicomplexa, toxoplasma gondii is an obligate intracellular parasite that secretes several classes of proteins involved in the active invasion of target host cells. proteins in apical secretory organelles known as micronemes have been strongly implicated in parasite attachment to host cells. tgmic2 is a microneme protein with multiple adhesive domains that bind target cells and is mobilized onto the parasite surface during parasite attachment. ... | 2001 | 11532123 |
| isolation and characterization of two parasitic protozoa from a pacific harbor seal (phoca vitulina richardsi) with meningoencephalomyelitis. | two species of protozoans were isolated from a harbor seal with fatal meninogoencephalitis. serologic reactivity was detected to both sarcocystis neurona and toxoplasma gondii. parasites associated with brain inflammation and necrosis reacted only with immunohistochemical stains utilizing polyclonal antisera raised against sarcocystis neurona. however, 2 distinct parasites were observed in cell cultures derived from the seal's brain tissue. these parasites were separated by mouse passage and lim ... | 2001 | 11534647 |
| prevalence of neospora caninum infection in dogs from beef-cattle farms, dairy farms, and from urban areas of argentina. | prevalence of anti-neospora caninum antibodies was determined in sera of 320 dogs from argentina using the indirect fluorescent antibody test (ifat). antibodies to n. caninum were found in 121 of 320 (37.8%) sera with titers of 1:50 (21 dogs), 1:100 (23 dogs), 1:200 (23 dogs), 1:400 (17 dogs), 1:800 (23 dogs), and > or = 1:1.600 (14 dogs). the seropositivity (ifat, > or = 1:50) was higher in dogs from dairy (48% of 125) and beef (54.2% of 35) farms than in dogs from urban areas (26.2% of 160). p ... | 2001 | 11534656 |
| oocyst excretion in dogs fed mouse brains containing tissue cysts of a cloned line of neospora caninum. | neospora caninum is an apicomplexan parasite that causes neonatal neuromuscular disease in dogs and abortions in cattle. bovine neosporosis is a major production problem worldwide. the parasite is transmitted to cattle via oocysts excreted by dogs or by transplacental transmission. dogs are the only proven definitive host for n. caninum. one of 3 dogs fed mouse brains containing tissue cysts of a wild-type n. caninum strain ck0160sc3b (cko) excreted oocysts in its feces. two of 3 dogs fed mouse ... | 2001 | 11534658 |
| association between exposure to neospora caninum and milk production in dairy cows. | to determine association between exposure to neospora caninum and milk production in dairy cows. | 2001 | 11549092 |
| neospora caninum microneme protein ncmic3: secretion, subcellular localization, and functional involvement in host cell interaction. | in apicomplexan parasites, host cell adhesion and subsequent invasion involve the sequential release of molecules originating from secretory organelles named micronemes, rhoptries, and dense granules. microneme proteins have been shown to be released at the onset of the initial contact between the parasite and the host cell and thus mediate and establish the physical interaction between the parasite and the host cell surface. this interaction most likely involves adhesive domains found within th ... | 2001 | 11553593 |
| protease activity and host cell binding of the 42-kda rhoptry protein from toxoplasma gondii after secretion. | three proteases were identified in the excretory/secretory proteins (esp) from toxoplasma gondii by the gelatin acrylamide gel electrophoresis (gage), of which the molecular masses were 80, 70, and 42 kda. one of the proteases with 42 kda was reactive to a monoclonal antibody (mab), tg786 clone, which was localized in the rhoptry of t. gondii by immunohistochemistry. the protease was maximally active at the ph range between 7.5 and 8.5, and was sensitive to inhibition by tpck and egta. the gelat ... | 2001 | 11563841 |
| ranch-management factors associated with antibody seropositivity for neospora caninum in consignments of beef calves in texas, usa. | a study was conducted with a 1998 retained-ownership population of texas (usa) beef calves to determine the ranch-management practices associated with calf seroprevalence to neospora caninum. management practices of 76 texas ranches that consigned 760 calves to a retained-ownership feedlot program were reviewed from a mailed questionnaire. ninety-nine of 760 (13%; 95% ci, 9.4%, 17.7%) calves were positive to n. caninum and 59% of the ranches consigned at least one positive calf. in the logistic ... | 2001 | 11566378 |
| epidemiological pattern and risk factors associated with bovine viral-diarrhoea virus (bvdv) infection in a non-vaccinated dairy-cattle population from the asturias region of spain. | a survey of bovine viral-diarrhoea virus (bvdv) infection was carried out in a non-vaccinated cattle population from the asturias region of spain in 1997 to assess seroprevalence and identify risk factors associated with infection. twenty-eight herds were included; 529 cows were bled. information regarding the herd and each animal sampled were recorded through a personal interview with the farmer. the true prevalence was estimated to be 21%. according to the antibody-age profiles and the herd-ma ... | 2001 | 11566379 |
| seroprevalence study of bovine neosporosis in mexico. | an indirect enzyme-linked immunosorbent assay was used to obtain epidemiologic information on bovine neosporosis in dairy herds of the mexican central plateau. sera were collected from 1,003 cows from 50 dairy herds. forty-three herds (group a) had been experiencing a high abortion rate. the abortion rates for the remaining 7 herds (group b) were within normal limits for mexico. five-hundred sixty-one (56%) of the 1,003 sera were positive. the seroprevalence of neospora caninum antibodies was 72 ... | 2001 | 11580063 |
| granulomatous encephalitis in a neurologically impaired goat kid associated with degeneration of neospora caninum tissue cysts. | congenital neospora caninum infection was diagnosed in a saanen goat from a farm in southern brazil. the kid was unable to nurse and had difficulty rising, ataxia, and opistothotonos. the neurologic signs became more severe 3 days after birth, when it was euthanized. no gross lesions were observed at necropsy. multifocal infiltrates primarily of mononuclear cells, nodular microgliosis, and perivascular cuffs of lymphocytes, plasma cells, and few neutrophils were observed in the brain, mostly in ... | 2001 | 11580064 |
| isolation of neospora caninum from the brain of a naturally infected dog, and production of encysted bradyzoites in gerbils. | neospora caninum was isolated from the brain of an adult dog in brazil. cerebral tissue from the dog was inoculated into mongolian gerbils. gerbils were euthanized 3-4 months later and bradyzoite-containing tissue cysts were observed in their brains. n. caninum (designated nc-bahia) was isolated in cell culture after inoculation with tissue cysts from the gerbils. the identity of the parasite was confirmed by immunohistochemical examination and polymerase chain reaction (pcr). gerbils may be a u ... | 2001 | 11587828 |
| determination of antigenic domain in gst fused major surface protein (nc-p43) of neospora caninum. | the antigenic domain of the major surface protein (nc-p43) of neospora caninum was examined by polymerase chain reaction of its gene fragments and recombinant expression as gst fusion proteins. the fragments of nc-p43 were as follow: a total open reading frame (ofr), t; ofr without signal sequence and c-terminal hydrophobic sequence, s; n-terminal 2/3 parts of s, a; c-terminal 2/3 parts, p; n-terminal 1/3 part, x; middle 1/3 part, y; and c-terminal 1/3 part, z, respectively. the dna fragments we ... | 2001 | 11590914 |
| protection against vertical transmission in bovine neosporosis. | in this study we were interested to determine whether infection of cattle prior to pregnancy would afford any protection to the foetus if the dams were challenged with neospora caninum at mid-gestation. the experiment comprised four groups of cattle: group 1, uninfected controls; group 2, inoculated with n. caninum tachyzoites 6 weeks prior to mating and then challenged with n. caninum at mid-gestation; group 3, naive cattle challenged with n. caninum at mid-gestation and group 4 were infected w ... | 2001 | 11595240 |
| validation of a commercially available monoclonal antibody-based competitive-inhibition enzyme-linked immunosorbent assay for detection of serum antibodies to neospora caninum in cattle. | a previously described monoclonal antibody (mab)-based competitive-inhibition enzyme-linked immunosorbent assay (celisa) was modified to optimize performance, and the assay was validated in various defined cattle populations for detection of serum antibody to neospora caninum, a major cause of bovine abortion. modifications to the celisa included capturing native n. caninum antigen with a parasite-specific mab (mab 5b6-25) and directly conjugating the competitor mab (mab 4a4-2), with both mabs b ... | 2001 | 11682497 |
| characterization of neospora caninum surface protein ncsrs2 based on baculovirus expression system and its application for serodiagnosis of neospora infection. | the baculovirus expression system has proved to be a useful tool for the production of recombinant proteins. here we have characterized the neospora caninum surface protein ncsrs2 produced by two types of the recombinant virus and also have developed an enzyme-linked immunosorbent assay (elisa) using recombinant ncsrs2 for the serologic diagnosis of neospora infection. western blot analysis showed two major protein bands that were detectable in insect cells infected with each recombinant baculov ... | 2001 | 11682519 |
| placentophagia--an alternative way for horizontal transmission of neospora caninum in cattle? | 2001 | 11756037 | |
| do cows protect fetuses from neospora caninum transmission? | 2001 | 11137733 | |
| protective efficacy of vaccination by recombinant vaccinia virus against neospora caninum infection. | the recombinant vaccinia viruses expressing the surface protein of neospora caninum tachyzoite, ncsag1 or ncsrs2, were constructed. the vaccination with these recombinant viruses could protect effectively the parasite invasion in a mouse model system. the vaccine efficacy of ncsrs2 was higher than that of ncsag1. the present study indicated that a high level of igg1 ab production to parasite is important for clearance of parasite at the early stage of infection and that t cell response has a cru ... | 2001 | 11163660 |
| isolation of neospora caninum genes detected during a chronic murine infection. | in order to isolate genes coding for antigens of neospora caninum which are recognised by the host immune system during a chronic murine infection, a cdna library was immunoscreened with pooled sera from mice which survived three independent infections by n. caninum. two new genes from n. caninum were isolated and expressed in escherichia coli. the genes identified include one homologous to gra1 of toxoplasma gondii, plus another (ncp20) previously unknown in any taxon. both genes encode small p ... | 2001 | 11165273 |
| prevention of vertical transmission of neospora caninum in balb/c mice by recombinant vaccinia virus carrying ncsrs2 gene. | neospora caninum infection is the major cause of bovine abortion. to develop a vaccine against n. caninum infection, recombinant vaccinia viruses carrying ncsrs2 and ncsag1 genes (vv/nc-p43 and vv/nc-p36, respectively) were constructed and were tested in a mouse model. vaccination of dams with vv/nc-p43 appeared to confer effective protection against vertical transmission to offspring, though that with vv/nc-p36 only provided partial protection. moreover, the vv/nc-p43 vaccination provoked cellu ... | 2001 | 11166895 |
| serological analysis of calves experimentally infected with neospora caninum: a 1-year study. | a serological study was conducted with calves experimentally infected with the protozoan parasite neospora caninum. the animals were inoculated with either a low or high dose of n. caninum tachyzoites and temperature responses monitored daily for the first 2 weeks after inoculation. blood samples were collected before inoculation, and at regular intervals thereafter for 1 year. serological analysis was achieved using an indirect fluorescent antibody test (ifat), an enzyme-linked immunosorbent as ... | 2001 | 11182233 |
| neospora caninum in persistently infected, pregnant cows: spontaneous transplacental infection is associated with an acute increase in maternal antibody. | nine cows which were naturally and persistently infected with neospora caninum were housed and observed intensively throughout pregnancy. no recrudescence of a latent infection was detected by pcr tests on maternal blood but fetal infection, implying a recrudescence of maternal parasitosis, was associated with a marked increase in maternal antibody. the increase occurred in the second half of pregnancy in five cows which infected their calves, and before mid-pregnancy in one cow which aborted. t ... | 2001 | 11688746 |
| hammondia heydorni-like oocysts shed by a naturally infected dog and neospora caninum nc-1 cannot be distinguished. | this study describes transmission experiments using hammondia heydorni-like oocysts isolated in 1996 from a naturally infected dog. the isolate was designated as h. heydorni-berlin-1996. examination of sera from infected intermediate hosts showed immunoblot reactions that resembled patterns observed after neospora caninum nc-1 infection. furthermore, n. caninum dna could be demonstrated in tissue samples (e.g. heart, brain) of experimentally infected intermediate hosts and in oocyst preparations ... | 2001 | 11688886 |
| immune responses in pregnant cattle and bovine fetuses following experimental infection with neospora caninum. | humoral and cell-mediated immune (cmi) responses [i.e. proliferative responses and gamma interferon (ifn-gamma) production], were elicited in five cows infected between 159 and 169 days of gestation by a combined intravenous-intramuscular inoculation of neospora caninum tachyzoites. analysis of antigen-specific immunoglobulin (igg) subclasses revealed a predominant igg2 response in two cows, a mixed igg1-igg2 response in two other cows and a predominant igg1 response in one cow. no correlation w ... | 2001 | 11688887 |
| cyclic transmission of neospora caninum: serological findings in dogs shedding oocysts. | in a previous paper we demonstrated that hammondia heydorni-like oocysts isolated in 1996 from a naturally infected dog could not be distinguished from the isolate neospora caninum nc-1. the isolate, designated as h. heydorni-berlin-1996, was cyclically transmitted using dogs as the final hosts. the present study provides information on the antibody responses of the dogs used for the cyclical transmission of this isolate. the majority of dogs that had shed oocysts showed no sero-conversion with ... | 2001 | 11688895 |
| comparative molecular investigation of nc5-pcr amplicons from neospora caninum nc-1 and hammondia heydorni-berlin-1996. | the clinical relevance of neospora caninum as a cyst-forming coccidian parasite is increasingly acknowledged within veterinary medicine, although the pathways of transmission are far from being solved. the parasite is well known for causing diaplacental infections in cows associated with abortion and/or severe damage of the fetus. in addition, it may cause neuromuscular disease in dogs, which thus apparently act as intermediate hosts as well as final hosts. in our previous studies, we have demon ... | 2001 | 11688897 |
| prevalence of agglutinating antibodies to neospora caninum in raccoons, procyon lotor. | neospora caninum is an apicomplexan parasite that causes neonatal neuromuscular disease in dogs and abortions in cattle. dogs are the only proven definitive host. little is known about the prevalence of antibodies to this parasite in wildlife. sera from 99 raccoons (procyon lotor) were examined for agglutinating antibodies to n. caninum using the modified agglutination test employing formalin-fixed tachyzoites as antigen. raccoons originated in florida (n = 24, collected in 1996), new jersey (n ... | 2001 | 11695399 |
| association of serologic status for neospora caninum and postweaning feed efficiency in beef steers. | to determine the effects of serologic status for neospora caninum on short-term weight gain, feed intake, and feed efficiency (feed intake/gain). | 2001 | 11697371 |