Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| evaluation of four serological techniques to determine the seroprevalence of neospora caninum in foxes (vulpes vulpes) and coyotes (canis latrans) on prince edward island, canada. | the objectives of this study were (1) to evaluate the performance and agreement of serological assays (elisa, ifat, neospora caninum agglutination test and immunoblot) using reference sera and field sera from foxes and coyotes and (2) to estimate the n. caninum seroprevalence in foxes and coyotes on prince edward island, canada. with fox and coyote reference sera the test performance of the elisa, ifat and ib was excellent (100% sensitivity and specificity). nat showed a low sensitivity (50%). s ... | 2007 | 17223270 |
| evaluation of serological tests for the diagnosis of neospora caninum infection in dogs: optimization of cut off titers and inhibition studies of cross-reactivity with toxoplasma gondii. | diagnosis of neospora caninum infection in dogs is based on serological assays such as the indirect fluorescent antibody test (ifat) and enzyme-linked immunosorbent assays (elisa). this study evaluated two serological tests (ifat and elisa) for the detection of igg antibodies to n. caninum in 300 serum samples of dogs through the optimization of cut off titers by using the two-graph receiver-operating characteristic (tg-roc) curve. in addition, the identification of major cross-reactive antigens ... | 2007 | 16973287 |
| serological survey of neospora caninum in dogs in the czech republic and a long-term study of dynamics of antibodies. | the main aim of the present study was to establish the prevalence of antibodies against neospora caninum dogs from the czech republic and to examine the dynamics of antibody titers during a long-term period. for this purpose, sera of 858 dogs were examined for the presence of anti-n. caninum antibodies using an indirect immunofluorescent antibody test (ifat). four groups of dogs of various origins were included in the survey: the first group (a, n=470) comprised dogs purchased by the czech army ... | 2007 | 16956728 |
| neospora caninum detected in feral rodents. | the role of rodents in the epidemiology of neosporosis was investigated by assaying brain tissue of feral mice (mus musculus) and rats (rattus norvegicus) for neospora caninum. both mouse and rat brain tissue were extracted for total dna, and subjected to two different n. caninum-specific nested polymerase chain reaction (pcr) assays. a portion of brain tissue from the mice and rats were also assayed for n. caninum in gerbils or gamma-interferon gene knockout (ko) mice. of the 105 feral mice tes ... | 2007 | 16997474 |
| serodiagnosis of neospora caninum infection in cattle using a recombinant tncsrs2 protein-based elisa. | the truncated ncsrs2 gene (tncsrs2) by removal of the n-terminal hydrophobic sequence was cloned into the pgex-6p-1 plasmid and subsequently expressed as a glutathione-s-transferase (gst) fusion protein. the purified recombinant tncsrs2 protein was specific to neospora caninum and was used in an elisa for the diagnosis of neosporosis. there was a good agreement between tnctsrs2-based elisa and three commercially available diagnostic kits (idexx elisa, hipra elisa and vmrd ifat). three hundred da ... | 2007 | 16989950 |
| first report of neospora caninum-associated bovine abortion in mashhad area, iran. | neospora caninum is an apicomplexan protozoan which causes abortion in cattle worldwide. the present study was designed to assess the importance of bovine neosporosis for causing abortion in iranian cattle. infection was primarily diagnosed by polymerase chain reaction (pcr), complemented with histopathology and immunohistochemistry (ihc). one hundred brains of aborted bovine fetuses were collected from iranian dairy herd between 2003 and 2005 in mashhad area. n. caninum was detected by pcr in t ... | 2007 | 17024355 |
| comparison of serological methods for the diagnosis of neospora caninum infection in cattle. | the aims of this study were to evaluate the performance and agreement of various commercial and in-house neospora caninum antibody assays used in dairy cattle in north america, and to investigate reproducibility of two assays performed in different laboratories. from 1998 to 2005, three enzyme linked immunosorbent assays (elisas, a competitive elisa-vmrd inc., an indirect elisa-biovet inc., and another indirect elisa-herdchek idexx corp.), two indirect fluorescent antibody tests (ifats, vmrd inc ... | 2007 | 16989951 |
| prevalence of antibodies against toxoplasma gondii and neospora caninum in hungarian red foxes (vulpes vulpes). | in the present study we have investigated the seroprevalence to the protozoan parasites toxoplasma gondii and neospora caninum in 337 red foxes (vulpes vulpes) from 16 out of 19 counties in hungary. the foxes were originally collected within a national vaccination program against rabies. antibodies to t. gondii were detected in as many as 228 (68%) of the foxes using a commercial direct agglutination test (dat). in an indirect iscom elisa, five foxes (1.5%) were positive for antibodies against n ... | 2007 | 17045742 |
| survey of neospora caninum and bovine herpes virus 1 coinfection in cattle. | a seroprevalence survey of neospora caninum and bovine herpesvirus 1 (bhv-1) was conducted in cattle pasturing in an area of the southern italian apennines to investigate the coinfection of these two pathogens. blood samples were collected from 948 pastured cattle raised on 81 farms. sera were tested for antibodies to n. caninum and to bhv-1 using an elisa assay and a neutralization test, respectively. out of the 81 farms sampled, 63 (77.8%) were positive for n. caninum and 80 (98.8%) for bhv-1. ... | 2007 | 17053931 |
| plasma pregnancy-associated glycoprotein-1 (pag-1) concentrations during gestation in neospora-infected dairy cows. | the aim of this study was to determine whether plasma pregnancy-associated glycoprotein-1 (pag-1) concentrations in pregnancy are affected by persistent neospora caninum infection in dairy cows. the data analyzed were derived from 22 multiparous cows: 16 n. caninum-seropositive and 6 n. caninum-seronegative animals (used as controls). three of the 16 seropositive cows aborted during the study period and the corresponding data were analyzed separately. pregnancy diagnoses were performed on day 40 ... | 2007 | 17010415 |
| the first detection of neospora caninum dna in the colostrum of infected cows. | limited data is available on the vertical transmission of neospora caninum via the colostrum. the results of our previous research revealed the presence of n. caninum dna in the milk of seropositive cows. the aim of the present work is to demonstrate parasite dna in colostrum samples. a polymerase chain reaction using np21 and np6 primers was applied to dna isolated from the colostrum sediment in order to amplify the corresponding genomic nc-5 region. the expected 328-bp product was obtained in ... | 2007 | 17024360 |
| intrauterine neospora caninum inoculation of heifers and cows using contaminated semen with different numbers of tachyzoites. | to investigate the potential of different neospora caninum tachyzoite doses to infect heifers (experiment 1) and cows (experiment 2) when administered in utero by artificial insemination via contaminated semen. | 2007 | 17126895 |
| pcr-rflp for the detection and differentiation of the canine piroplasm species and its use with filter paper-based technologies. | canine piroplasmosis is an emerging disease worldwide, with multiple species of piroplasm now recognised to infect dogs. a nested polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) assay was developed for the detection and differentiation of each of the piroplasm species currently known to infect dogs on the basis of the 18s ribosomal rna gene. the assay can potentially amplify and discriminate between theileria annae, theileria equi, babesia conradae, babesia gibsoni, ... | 2007 | 17127005 |
| multimammate rat (mastomys natalensis), tristram's jird (meriones tristrami) and wagner's gerbil (gerbillus dasyurus) as laboratory models of acute neosporosis. | to test the different sensitivity of rodents of the subfamily murinae and gerbillinae, wagner's gerbils (gerbillus dasyurus), tristram's jirds (meriones tristrami) and multimammate rats (mastomys natalensis) were inoculated with neospora caninum tachyzoites. clinical signs of neosporosis appeared in all inoculated animals. histopathological examination confirmed the presence of tachyzoites in brains, lungs, skeletal muscle, myocardium, liver, in serosa of stomach and intestines, and in vesicular ... | 2007 | 17134728 |
| prevalence of neospora caninum and toxoplasma gondii in sheep and dogs from guarapuava farms, paraná state, brazil. | sheep and dog blood samples were collected from nine farms in the county of guarapuava, paraná, brazil. the indirect fluorescent antibody test (ifat) was used to detect neospora caninum and toxoplasma gondii antibodies. herein, serum samples from 305 sheep were evaluated, being 29 (9.5%) and 157 (51.5%) seropositives to n. caninum and t. gondii, respectively. seven (29.1%) and five (20.8%) out of 24 dogs were seropositives to n. caninum and t. gondii, respectively. there were no differences amon ... | 2007 | 17266999 |
| serologic study of anti-neospora caninum antibodies in household dogs and dogs living in dairy and beef cattle farms in tehran, iran. | a few studies have been done on the seroepidemiology of anti-neospora caninum antibodies in dairy and beef cattle farms in iran, which suggested the presence of n. caninum in these areas, but there is no published information directed on the presence or epidemiology of this organism in the dogs in iran. to investigate anti-n. caninum antibodies in household dogs and dogs living in cattle farms, 100 blood samples were collected: 50 from dogs living in dairy and beef cattle farms and 50 from house ... | 2007 | 17120042 |
| the ncgra7 gene encodes the immunodominant 17 kda antigen of neospora caninum. | a neospora caninum 17-19 kda antigenic protein fraction (p17) in one-dimensional polyacrylamide gel electrophoresis (sds-page) is the immunodominant antigen recognized by sera from bovines naturally infected by n. caninum. to identify the proteins making up the p17 fraction, we screened a new n. caninum tachyzoite cdna library with an affinity-purified antibody against p17 (apa17). we isolated several cdna clones with 100% sequence identity to the ncgra7 gene. this previously described gene enco ... | 2007 | 17032479 |
| structure-activity relationships from in vitro efficacies of the thiazolide series against the intracellular apicomplexan protozoan neospora caninum. | nitazoxanide (2-acetolyloxy-n-(5-nitro 2-thiazolyl) benzamide; ntz) represents the parent compound of a novel class of broad-spectrum anti-parasitic compounds named thiazolides. ntz is active against a wide variety of intestinal and tissue-dwelling helminths, protozoa, enteric bacteria and a number of viruses infecting animals and humans. while potent, this poses a problem in practice, since this obvious non-selectivity can lead to undesired side effects in both humans and animals. in this study ... | 2007 | 17141783 |
| immunization of cattle with live tachyzoites of neospora caninum confers protection against fetal death. | neospora caninum is an obligate intracellular protozoan parasite that causes abortion in cattle. it is normally found as a latent infection controlled by a t-helper-cell type 1 response involving cd4(+) cytotoxic t cells and gamma interferon. cattle may be infected by two different routes: transplacentally as a result of activation of the latent infection in the mother causing congenital infection or abortion and by ingestion of oocysts, which, if it occurs during gestation, can also result in a ... | 2007 | 17145943 |
| neospora caninum emerges from the shadow of toxoplasma gondii. | it is sometimes easy to make the mistake of assuming that everything that holds true for toxoplasma gondii is also true for its relative neospora caninum. however, a recurring theme in the recent review by hemphill et al. is not the similarities but the striking differences between the two parasites. | 2007 | 17185039 |
| natural killer cells act as early responders in an experimental infection with neospora caninum in calves. | the intracellular protozoan parasite neospora caninum is a cause of abortion and congenital disease in cattle worldwide. we have previously shown that natural killer (nk) cells produce ifn-gamma in response to n. caninum tachyzoites in vitro. this study aimed to investigate the role of nk cells and other cellular immune responses in an experimental n. caninum infection model in calves. phenotyping of peripheral blood lymphocytes showed a drop in the percentage of nk cells at days 4-6 after i.v. ... | 2007 | 17188277 |
| risk factors for canine neosporosis in farm and kennel dogs in southern italy. | neosporosis by neospora caninum causes losses to livestock production through abortion in cattle while, in dogs, it induces neuromuscolar disease. this study investigated neosporosis seroprevalence associated risk factors (including the seropositivity to leishmaniosis) in dogs of southern italy, determined the prevalence of n. caninum oocyst shedding and examined the relationship between seroprevalence of neosporosis in farm dogs and cattle. using an inhibition elisa, 20.9% of 306 dogs had perce ... | 2007 | 17257762 |
| production effects of pathogens causing bovine leukosis, bovine viral diarrhea, paratuberculosis, and neosporosis. | the primary purpose of this research was to determine associations among seropositivity for bovine leukemia virus (blv), bovine viral diarrhea virus (bvdv), mycobacterium avium ssp. paratuberculosis (map), and neospora caninum (nc) and each of 3 outcome variables (305-d milk, fat, and protein production) in canadian dairy cattle. serum samples from up to 30 randomly selected cows from 342 herds on monthly milk testing were tested for antibodies against blv (idexx elisa; idexx corporation, westbr ... | 2007 | 17235141 |
| immunoanalysis of three litters born to a doberman bitch infected with neospora caninum. | neospora caninum is a tissue cyst-forming coccidium that may cause neuromuscular disorders in dogs. infected bitches can transmit the parasite to their pups in utero. vertical transmission may occur after primary infection during pregnancy and in subsequent pregnancies. the reason why only a few pups develop clinical neosporosis is unknown. we obtained sera from a doberman bitch and its offspring delivered in three litters. the bitch had a titer of 1:640 in an indirect fluorescent antibody test ... | 2007 | 17096148 |
| neospora caninum antibodies detected in midwestern white-tailed deer (odocoileus virginianus) by western blot and elisa. | white-tailed deer (odocoileus virginianus) serve to maintain the neospora caninum life cycle in the wild. sera from white-tailed deer from south central wisconsin and southeastern missouri, usa were tested for antibodies to n. caninum by western blot analyses and two indirect elisas. seroreactivity against n. caninum surface antigens was observed in 30 of 147 (20%) of wi deer and 11 of 23 (48%) of mo deer using western blot analysis. compared to western blot, the two indirect elisas were found t ... | 2007 | 17194547 |
| apical membrane antigen 1 is a cross-reactive antigen between neospora caninum and toxoplasma gondii, and the anti-ncama1 antibody inhibits host cell invasion by both parasites. | the cross-reactive antigens of neospora caninum and toxoplasma gondii are important in the exploration to determine the common mechanisms of parasite-host interaction. in this study, a gene encoding n. caninum apical membrane antigen 1 (ncama1) was identified by immunoscreening of a n. caninum tachyzoite cdna expression library with antisera from mice immunized with recombinant t. gondii apical membrane antigen 1 (tgama1). ncama1 was encoded by an open reading frame of 1695 bp, which encoded a p ... | 2007 | 17156863 |
| progesterone supplementation during mid-gestation increases the risk of abortion in neospora-infected dairy cows with high antibody titres. | progesterone has been reported to reduce th1 responses during pregnancy. thus, given that an excessive th1 response could lead to abortion in neospora-infected dairy cows, we hypothesized a reduction in abortion risk following progesterone supplementation during the mid-gestation period in these animals. seropositive animals were assigned on day 120 of gestation to a control (n=33) or treatment (n=34) group. treatment consisted of progesterone supplementation by fitting a progesterone releasing ... | 2007 | 17182187 |
| host/parasite relationship in the in vitro infection of rat gliocytes by neospora caninum: evaluation of cell respiration. | neospora caninum is a protozoon that causes abortion in cattle and neuromuscular lesions in dogs, with the formation of cysts mainly in the central nervous system. since n. caninum is an intracellular parasite with tropism for the cells of nervous system, this study evaluated the respiratory metabolism of glial cells infected by this* parasite. glial cultures obtained from the cerebral cortex of newborn rats were kept in dmem enriched with 10% fetal bovine serum, 1 mm pyruvic acid and 2 mm of l- ... | 2007 | 17184800 |
| analysis of the immune response to neospora caninum in a model of intragastric infection in mice. | to study experimental neospora caninum infection initiated at the gastrointestinal tract, toll-like receptor 4- and functional il-12rbeta2 chain-deficient c57bl/10 sccr mice were challenged intragastrically with 5 x 10(6) n. caninum tachyzoites. all parasite-inoculated mice eventually died with disseminated infection. in contrast, immunocompetent balb/c mice challenged with 1 x 10(7) n. caninum tachyzoites by the intragastric (i.g.) or the intraperitoneal (i.p.) route remained alive for at least ... | 2007 | 17187652 |
| quantification of vertical and horizontal transmission of neospora caninum infection in dutch dairy herds. | ninety-six of 108 randomly selected dutch dairy herds had one or more cows with a positive serostatus for n. caninum. in these 96 herds, we have quantified the probabilities of vertical transmission (vt) and horizontal transmission (ht) of n. caninum infection by combining serostatus and pedigree data in 4091 dam-daughter pairs. the probability of animals infected by vertical transmission during pregnancy (prob(vt)) was calculated as the proportion of seropositive daughters among daughters of se ... | 2007 | 17640807 |
| dynamics of anti-neospora caninum antibodies during gestation in chronically infected dairy cows. | the dynamics of antibody production against neospora caninum during the gestation period was examined in chronically infected dairy cows. data were obtained from 86 pregnant parous dairy cows, 21 of which had suffered abortion. the cows belonged to two herds in which a diagnosis of n. caninum infection had been previously confirmed in aborted foetuses. pregnancy diagnosis and blood collection were performed on post-insemination days 40, 90, 120, 150, 180, 210, and at parturition or until the tim ... | 2007 | 17643822 |
| seroepidemiology of toxoplasma gondii and neospora caninum in seals around hokkaido, japan. | serological analysis was performed to detect toxoplasma gondii and neospora caninum infection in seals in hokkaido. serum samples were collected from 322 kuril harbor seals (phoca vitulina stejnegeri) at nosappu, akkeshi and erimo, from 46 spotted seals (p. largha) at nosappu, erimo, yagishiri island, hamamasu and syakotan, and from 4 ribbon seals (p. fasciata) and a bearded seal (erignathus barbatus) at nosappu between 1998 and 2006. recombinant surface antigen of t. gondii (sag2t) and n. canin ... | 2007 | 17485927 |
| reduced cerebral infection of neospora caninum in balb/c mice vaccinated with recombinant brucella abortus rb51 strains expressing n. caninum srs2 and gra7 proteins. | neospora caninum, an obligate intracellular protozoan parasite, is the causative agent of bovine neosporosis, an important disease affecting the reproductive performance of cattle worldwide. currently there is no effective vaccine available to prevent n. caninum infection in cattle. in this study, we examined the feasibility of developing a live, recombinant n. caninum vaccine using brucella abortus vaccine strain rb51 as the expression and delivery vector. we generated two recombinant rb51 stra ... | 2007 | 17651896 |
| evidence of neospora caninum dna in wild rodents. | seventy-five house mice (mus musculus), 103 rats (rattus norvegicus) and 55 field mice (apodemus sylvaticus) from north-west italy were pcr analysed for neospora caninum infection. brain, kidney and muscle tissues collected from the above mentioned animals were tested by pcr using np6 and np21 primers. the brain tissue from 2 house mice and 2 rats, the kidney from 4 rats, 1 house mouse and 1 field mouse and muscle from 10 rats, 8 house mice and 1 field mouse were tested positive for n. caninum. ... | 2007 | 17651897 |
| seroprevalence of neospora caninum infection in dogs from rural and urban environments in tehran, iran. | 103 serum samples were taken from owned dogs in tehran (53 urban dogs and 50 farm dogs) from february to may 2005 and tested for igg antibody against neospora caninum using the immunofluorescence antibody test. urban dogs were selected from apparent healthy dogs referred to the small animal hospital of faculty of veterinary medicine, university of tehran, for routine operation, and the farm dogs were selected from the sheepdogs in sheep flocks around tehran randomly. the titer of 1:50 was select ... | 2007 | 17687566 |
| isolation and molecular detection of neospora caninum from naturally infected sheep from brazil. | neospora caninum was isolated from a naturally infected sheep from brazil by bioassay in dogs. approximately 70g of brain from each of two 4-month-old sheep with indirect fluorescent antibodies (>or=1:50) to n. caninum was offered to a different ifat negative dog (sheep n. 302, ifat 1:400-dog 1 and sheep n. 342, ifat 1:50-dog 2). parasite dna was detected in both sheep brains using a pcr targeting the nc-5 gene of n. caninum. shedding of neospora-like oocysts was noticed only in dog 1, from 10 d ... | 2007 | 17451882 |
| prevention of vertical transmission of neospora caninum in c57bl/6 mice vaccinated with brucella abortus strain rb51 expressing n. caninum protective antigens. | bovine abortions caused by the apicomplexan parasite neospora caninum have been responsible for severe economic losses to the cattle industry. infected cows either experience abortion or transmit the parasite transplacentally at a rate of up to 95%. neospora caninum vaccines that can prevent vertical transmission and ensure disruption in the life cycle of the parasite greatly aid in the management of neosporosis in the cattle industry. brucella abortus strain rb51, a commercially available vacci ... | 2007 | 17575983 |
| the apicomplexan pathogen neospora caninum inhibits host cell apoptosis in the absence of discernible nf-kappa b activation. | neospora caninum, a causative agent of bovine abortions, is an apicomplexan parasite that is closely related to the human pathogen toxoplasma gondii. since a number of intracellular parasites, including t. gondii, have been shown to modulate host cell apoptosis, the present study was conducted to establish whether n. caninum is similarly capable of subverting apoptotic pathways in its host cells. our results indicated that death receptor-mediated apoptosis is repressed during n. caninum infectio ... | 2007 | 17576757 |
| possible role of bovine trophoblast giant cells in transplacental transmission of neospora caninum in cattle. | neospora caninum is an aplicomplexan parasite that has brought several concerns to cattle raisers worldwide due to its relationship to fetal loss. however, the mechanism of the parasite's transplacental infection and induced abortions are not completely understood. bovine trophoblastic binucleated cells (bnc) play a major role in the maternal-fetal interactions, migrating during the entire pregnancy from chorionic connections to uterine epithelium. this study aimed to investigate the possible ro ... | 2007 | 17588318 |
| prevention of lethal experimental infection of c57bl/6 mice by vaccination with brucella abortus strain rb51 expressing neospora caninum antigens. | bovine abortions caused by the intracellular protozoal parasite neospora caninum are a major concern to cattle industries worldwide. a strong th1 immune response is required for protection against n. caninum. brucella abortus strain rb51 is currently used as a live, attenuated vaccine against bovine brucellosis. strain rb51 can also be used as an expression vector for heterologous protein expression. in this study, putative protective antigens of n. caninum mic1, mic3, gra2, gra6 and srs2, were ... | 2007 | 17568587 |
| development of two immunochromatographic tests for the serodiagnosis of bovine babesiosis. | in this study, we developed two immunochromatographic tests (icts), which are nitrocellulose membrane-based immunoassays for the convenient and rapid serodiagnosis of bovine babesiosis caused by babesia bovis (boict) and babesia bigemina (biict). the efficacy of two icts was evaluated using 13 positive sera from experimentally infected cattle with b. bovis or b. bigemina. clear results showed that the boict and elisa detected antibodies in sera collected from 14 to 93 days post-infection, while ... | 2007 | 17590275 |
| factors associated with variation in neospora caninum bulk-milk s/p ratios in initially bulk-milk negative testing dutch dairy herds. | we conducted a study on 81 initially bulk-milk elisa negative dairy herds taken from a random sample of dutch dairy herds to evaluate variation in bulk-milk s/p ratios and to study reasons for bulk-milk conversion. these herds were repeatedly (3-month intervals) tested between april 2004 and august 2005 and serostatus of all animals had previously been established as negative (n), low-positive (lp) or high-positive (hp). of these herds, herd- and test-related factors associated with variation in ... | 2007 | 17566576 |
| possibility of neospora caninum infection by venereal transmission in cb-17 scid mice. | cb-17 scid and balb/c male mice were inoculated intraperitoneally with neospora caninum to examine the possibility of its venereal transmission. some of these mice were killed on days 7 and 20 post-inoculation to examine the genital organs for presence of the parasite. the remaining scid male mice were housed with non-infected female mice from day 7 p.i. and kept with them for 14 days. these scid mice died between days 28 and 35 p.i. n. caninum dna was detected in the testis of mice on days 7 an ... | 2007 | 17689194 |
| prevalence of anti-toxoplasma gondii and anti-neospora caninum antibodies in goats slaughtered in the public slaughterhouse of patos city, paraíba state, northeast region of brazil. | the prevalence of anti-toxoplasma gondii and anti-neospora caninum antibodies was investigated in goats slaughtered in the public slaughterhouse of patos, state of paraíba, northeast region of brazil, and possible associations between sex of the animals and antibody prevalence were verified. three-hundred six blood samples from goats collected before slaughter by jugular venopuncture were used. for the serologic diagnosis of t. gondii and n. caninum, the indirect fluorescent-antibody test (ifat) ... | 2007 | 17706359 |
| sarcocystis sp.-associated meningoencephalitis in a bald eagle (haliaeetus leucocephalus). | protozoal meningoencephalitis is uncommon in raptors. an adult female bald eagle (haliaeetus leucocephalus) was euthanized after several months of treatment for progressive neurologic signs. the predominant histologic lesion was lymphoplasmacytic and histiocytic meningoencephalitis involving the cerebrum and cerebellum. there was a marked segmental loss of granular cells and purkinje cells, as well as segmental atrophy of the molecular layer in the cerebellum. protozoal merozoites and schizonts ... | 2007 | 17823405 |
| neospora caninum infection in birds: experimental infections in chicken and embryonated eggs. | neospora caninum causes economical impact in cattle-raising farms since it is implicated as the major cause of bovine abortions. although infection by the parasite has been widely described in mammals, the role of birds in its life-cycle is still obscure. therefore, this work aimed to evaluate the infection by n. caninum in different chicken models. experimental infections were conducted in 7-day-old chicks, laying hens and embryonated eggs, where samples were analysed for parasite burden, igg a ... | 2007 | 17686190 |
| cloning and expression of an antigenic domain of a major surface protein (nc-p43) of neospora caninum. | neospora caninum is an obligate intracellular protozoan that can infect domestic and wild canids, as well as ruminants and equines. it was described in 1988 as causing neuromuscular alterations and death in dogs. recently, n. caninum has been the focus of considerable attention for its large impact on the dairy industry, given the economic losses related to breeding failures and to a decrease in productivity. elisa diagnosis of neosporosis has not been widely used in brazil, mostly because of th ... | 2007 | 17706005 |
| first identification of neospora caninum infection in aborted bovine foetuses in china. | the first identification of neospora caninum infection in the tissues of aborted bovine foetuses in china is reported. aborted foetuses were collected from 16 dams, and 12 of the dams had high serum antibody titres to n. caninum determined using an elisa test kit. the nc-5 gene of n. caninum was amplified from dna samples extracted from brains of four aborted foetuses using a neospora-specific pcr assay, confirming n. caninum infection in the aborted foetuses. histology and immunohistochemistry ... | 2007 | 17706874 |
| isolation of neospora caninum from dairy zero grazing cattle in israel. | first israeli neospora caninum isolates were obtained from brain tissues of aborted fetuses (ncis491 and ncis580) from dairy farms endemic for neosporosis and maintaining cattle on zero grazing. tissues from different parts of the fetus brains were used to infect vero cells. tachyzoites of n. caninum were first observed in cultures from days 30 and 32 after infection. to confirm the identity of the isolated parasites, dna extracts from brains and cultures were tested by pcr with specific primers ... | 2007 | 17850972 |
| protection against abortion linked to gamma interferon production in pregnant dairy cows naturally infected with neospora caninum. | many immunological aspects of pregnancy, such as the role played by gamma interferon (ifn-gamma) in abortion, are not well understood. neospora caninum is an intracellular protozoan considered to be among the main causes of abortion in cattle worldwide. the present study analyzes the interaction between ifn-gamma production and n. caninum infection in naturally infected pregnant cows. data were obtained from 126 pregnant cows: 86 seropositive and 40 seronegative for the parasite. pregnancy diagn ... | 2007 | 17854883 |
| histopathological and immunohistochemical aspects of neospora caninum diagnosis in bovine aborted fetuses. | lesions suggestive of protozoal infection such as nonsuppurative myositis, nonsuppurative myocarditis, nonsuppurative pneumonia associated with focal cellular necrosis and multifocal necrotizing nonsuppurative encephalitis were observed in 89 (34%) out of 258 aborted bovine fetuses. immunohistochemistry (ihc) revealed tachyzoites and cysts of neospora caninum in 55 (61.8%) of these 89 fetuses. several fetal variables were tested by multivariate logistic regression, in which increasing odds for p ... | 2007 | 17904290 |
| cutaneous toxoplasmosis in a female japanese cat. | a 16-year-old female japanese cat was presented with a single mammary-gland nodule approximately 3 cm in diameter. histologically, the nodule consisted of necrotizing granulomatous panniculitis, vasculitis, and mastitis, and contained free and clustered protozoal organisms. the organism was present in the cytoplasm of macrophages, fibroblasts, endothelial cells, and mammary-gland epithelia. the organism was positive for anti- toxoplasma gondii and anti- neospora caninum antibodies. electron micr ... | 2007 | 17846241 |
| evaluation of neospora caninum and toxoplasma gondii infections in alpaca (vicugna pacos) and llama (lama glama) aborted foetuses from peru. | the aim of this study was to investigate the participation of neospora caninum and toxoplasma gondii in abortion cases of peruvian llamas and alpacas. fifteen aborted foetuses were recovered from two main rearing areas of camelids in peru (central or south andean region). foetal histopathology was used to detect the presence of protozoal-associated lesions in target organs. n. caninum and t. gondii infections were confirmed by immunohistochemistry (ihc) combined with pcr and by pcr alone, respec ... | 2007 | 17931783 |
| humoral immune response in pregnant heifers inoculated with neospora caninum tachyzoites by conjunctival route. | the aim of this study was to compare systemic humoral immune responses in pregnant heifers inoculated with neospora caninum tachyzoites by conjunctival and intravenous routes. twenty nine heifers separated in three experimental groups were studied: group 1 (n=10 animals) and group 2 (n=9 animals) were inoculated with 10(8) of n. caninum tachyzoites by conjunctival and intravenous routes at 5th month of gestation, respectively; group 3 (n=10 animals) were non-inoculated control animals. an indire ... | 2007 | 17697754 |
| internal hydrocephalus and associated periventricular encephalitis in a young fox. | marked lateral ventricular enlargement associated with atrophic cerebral cortex and periventricular encephalitis is described in a 2-month-old fox affected by disorientation, generalized ataxia, difficulty in walking, circling, and blindness. clinical conditions progressed to stupor and spontaneous death within a few days. at necropsy, severe inflammatory and necrotizing lesions were observed in periventricular sites associated with diverticula and cleft formation in perithalamic areas and rhine ... | 2007 | 17846249 |
| occurrence of antibodies against neospora caninum in water buffaloes (bubalus bubalis) on four ranches in corrientes province, argentina. | the aim of the present work was to describe the occurrence of antibodies to neospora caninum in water buffaloes on four ranches located in corrientes province in the northeast of argentina. antibodies against n. caninum were determined in sera of 449 water buffaloes by using an indirect fluorescent antibody test (ifat). a bayesian logistic regression mixed model was used to quantify the strength of association between positive serological results to n. caninum and gender, age and category (calf, ... | 2007 | 17950535 |
| neospora caninum in cattle: experimental infection with oocysts can result in exogenous transplacental infection, but not endogenous transplacental infection in the subsequent pregnancy. | whilst it is presumed that infection of pregnant cattle with neospora caninum oocysts can provoke abortion and is the likely cause of epidemic abortion outbreaks, only two previous experiments have involved inoculation of pregnant cows with oocysts (and only one abortion was provoked in 22 pregnancies). here, we describe the oral oocyst challenge of 18 cows synchronously bred and inoculated precisely at 70 (n=6), 120 (n=6) and 210 (n=6) days in pregnancy with a nominal dose of 40,000 oocysts. on ... | 2007 | 17624353 |
| fecal and serological survey of neospora caninum in farm dogs in costa rica. | to detect oocysts of neospora caninum in dog feces and to determine the excretion pattern in dogs from specialized dairy farms in costa rica, a total of 265 fecal samples from 34 dogs were collected at intervals from february to august 2005. fecal samples were examined for n. caninum-like oocysts microscopically, by dna detection using the polymerase chain reaction (pcr), and by bioassay. n. caninum dna was detected by pcr in four fecal samples, twice from one dog, but oocysts were not detected ... | 2007 | 17868998 |
| neosporosis in beagle dogs: clinical signs, diagnosis, treatment, isolation and genetic characterization of neospora caninum. | clinical neosporosis was diagnosed in a litter of five pups born to a beagle bitch from virginia, usa. four of the pups developed limb weakness starting at 4 weeks of age. the dogs were suspected to have neosporosis based on clinical signs and empirically treated with clindamycin (75 mg, oral, twice daily, total 150 mg) starting at 9 weeks of age and the dosage was doubled at 13 weeks of age. antibodies to neospora caninum were detected in sera of the dam and pups when first tested serologically ... | 2007 | 17890012 |
| sarcocystis neurona encephalitis in a dog. | a 1.5-year-old male feist dog was presented to a veterinarian for reluctance to stand on the hind legs. treatment included dexamethasone and resulted in a favorable initial response, but posterior paresis returned and progressed to recumbency, hyperesthesia, and attempts to bite the owner. the dog was euthanized. the brain was negative for rabies by fluorescent antibody analysis. multiple foci of encephalitis were found in the cerebrum and particularly in the cerebellum. protozoa morphologically ... | 2007 | 18039914 |
| anti-neosporal igg and ige antibodies in canine neosporosis. | neospora caninum infection provokes neurological disorders, recurrent abortion and death in dogs and cattle. dogs are both intermediate and definitive host of n. caninum. thus, the development of sensitive and specific immunoassays to diagnose canine neosporosis is essential to control this disease. this work investigated serum anti-neosporal igg and ige antibodies in 140 dogs represented by 30 healthy animals (group i), 11 dogs showing acute n. caninum infection (group ii), 50 urban dogs with s ... | 2007 | 18035978 |
| dense-granule protein ncgra7, a new marker for the serodiagnosis of neospora caninum infection in aborting cows. | to investigate whether the production of an antigen-specific antibody is associated with neospora caninum-induced bovine abortion, 62 serum samples were tested with an enzyme-linked immunosorbent assay using the recombinant antigens ncsag1, ncsrs2, and ncgra7. our study suggested that ncgra7 would be a new marker for the serodiagnosis of n. caninum infection resulting in abortion. | 2007 | 17959821 |
| c57bl/6 mice infected with neospora caninum during administration of progesterone show bias toward type 2 immune response. | to clarify the role of progesterone in the development of immune responses during pregnancy against neospora caninum infection, c57bl/6 mice were given a progesterone pellet, and measured on interferon-gamma and interleukin-4 production following the infection. ifn-gamma production in the prescribed group was significantly lower than that in the intact group on day 40 post administration. il-4 producing cell population in the prescribed group was larger than that in the intact group. these resul ... | 2007 | 17984602 |
| prevalence of anti-neospora caninum antibodies in cattle from the state of mato grosso do sul, brazil. | neospora caninum is an obligate intracellular parasite that can infect domestic and wild canids, as well as ruminants and equines. it was described in 1988 and has been known as a major cause of abortion in bovines and neuromuscular alterations and death in dogs. to estimate the prevalence of bovine neosporosis in the 22 municipalities of the so-called estrato 1 subregion of the brazilian state of mato grosso do sul, blood samples were collected from cows aged 24 months and older, from december ... | 2007 | 18078599 |
| seroprevalence of neospora spp. among asymptomatic horses, aborted mares and horses demonstrating neurological signs in israel. | sera from 800 asymptomatic horses were examined for the presence of antibodies to neospora caninum by immunofluorescence antibody test (ifat). the presence of antibodies to n. caninum was also tested in sera from 52 mares that had aborted and 40 horses exhibiting neurological signs. a total of 95 (11.9%) of the 800 sera had antibodies for neospora. significantly higher seropositivity was obtained from horses that had neurological signs (21.2%) and from aborted mares (37.5%). there was significan ... | 2007 | 17614202 |
| the host-parasite relationship in pregnant cattle infected with neospora caninum. | the protozoan parasite neospora caninum is an important cause of reproductive disease in cattle worldwide. the dog is a definitive host for the parasite and the oocyst stage, shed in the faeces, is a source of infection for cattle through consumption of contaminated feed or water. in addition, transplacental transmission of n. caninum is a very efficient means of the parasite infecting a new host and this can occur in successive pregnancies and over several generations. neospora parasites may ca ... | 2007 | 17958926 |
| bayesian kriging of seroprevalence to mycobacterium avium subspecies paratuberculosis and neospora caninum in alberta beef and dairy cattle. | identifying spatial patterns of risk is important in the study of diseases with ecologic causes. furthermore, relatively complex hierarchical modeling is required to determine how factors that are organized across levels interact, such as how an ecologic cause interacts with farm management and with animal characteristics. the objective of this study was to map the risk for mycobacterium avium subspecies paratuberculosis (map - the causative agent of johne's disease) and neospora caninum (nc - t ... | 2007 | 18189052 |
| neosporosis and hammondiosis in dogs. | the dog is a definitive host of the protozoan parasite neospora caninum, and in many parts of the world, infection is relatively common as determined by serology. reported seroprevalences usually range from 0 to 20 per cent, however, reports of clinically affected dogs are infrequent. affected dogs are generally less than six months old and predominantly have signs of an ascending hindleg paralysis, with the associated lesions of polyradiculoneuritis and granulomatous polymyositis. although any ... | 2007 | 17547641 |
| prevalence of neospora caninum and toxoplasma gondii antibodies in wild ruminants from the countryside or captivity in the czech republic. | in the czech republic, sera from 720 wild ruminants were examined for antibodies to neospora caninum by screening competitive-inhibition enzyme-linked immunosorbent assay and confirmed by indirect fluorescence antibody test (ifat); the same sera were also examined for antibodies to toxoplasma gondii by ifat. neospora caninum antibodies were found in 14% (11 positive/79 tested) roe deer (capreolus capreolus), 14% (2/14) sika deer (cervus nippon), 6% (24/ 377) red deer (cervus elaphus), 1% (2/143) ... | 2007 | 18163361 |
| seroprevalence of neospora caninum antibodies in cattle and water buffaloes in india. | neospora caninum is now recognized as a major cause of abortion in cattle worldwide, but there is no report of n. caninum infection in cattle in india. serum samples from 427 dairy cattle and 32 dairy water buffaloes from 7 organized dairy farms located in punjab, india, were tested for n. caninum antibodies using a commercial monoclonal antibody-based competitive enzyme-linked immunosorbent assay (elisa). antibodies to n. caninum were found in 35 of 427 cattle from 6 of the 7 farms; 9.6% of cow ... | 2007 | 18314683 |
| neospora caninum: high susceptibility to the parasite in c57bl/10sccr mice. | c57bl/10sccr mice, lack toll-like receptor 4 and a functional interleukin-12 receptor. taking this into account, susceptibility of these mice to neospora caninum infection was assessed comparatively to that of immunocompetent c57bl/10scsn mice. c57bl/10sccr mice inoculated intraperitoneally with 5x10(5)n. caninum tachyzoites showed a high susceptibility to this parasite. all infected c57bl/10sccr mice were dead by day 8 post-infection whereas all control c57bl/10scsn mice survived this parasitic ... | 2007 | 16889774 |
| seroprevalence of neospora caninum in non-carnivorous wildlife from spain. | serum samples from 1034 non-carnivorous wildlife from spain were tested for antibodies to neospora caninum by competitive screening enzyme linked immunosorbent assay (elisa) and confirmed by an indirect fluorescent antibody test (ifat). high agreement was observed between results in both techniques (kappa value higher than 0.9). prevalences of n. caninum antibodies positive by both techniques were 11.8% of 237 red deer (cervus elaphus), 7.7% of 13 barbary sheep (ammotragus lervia), 6.1% of 33 ro ... | 2007 | 16962706 |
| seroepidemiology of neospora caninum and toxoplasma gondii in cattle and water buffaloes (bubalus bubalis) in the people's republic of china. | a seroepidemiological survey of neospora caninum and toxoplasma gondii in cattle and water buffaloes was carried out in the people's republic of china. serum samples were obtained from dairy (n=262, 9 herds in 9 provinces) and beef cattle (n=10, 1 herd) and water buffaloes (n=40) in china. all sera were tested for antibodies to n. caninum and t. gondii by an enzyme-linked immunosorbent assay (elisa) and an indirect agglutination test (iat), respectively. the overall seroprevalence of n. caninum ... | 2007 | 17010521 |
| antibodies to neospora caninum and toxoplasma gondii in domestic cats from brazil. | the occurrence of antibodies to neospora caninum and toxoplasma gondii was determined in 400 domestic cats (siamese, persian, and undetermined breeds) from the municipality of araçatuba, sao paulo, brazil, through the indirect fluorescence antibody test (ifat). of the 400 cats, 100 were seropositive to t. gondii (25%, titer > or =64) and 98 to n. caninum (24.5%, titer > or =16). the rate of seropositive cats for t. gondii was correlated with age (chi(2)=35.7; p<0.001), with a higher number of in ... | 2007 | 16941188 |
| serosurvey of ex situ giant pandas (ailuropoda melanoleuca) and red pandas (ailurus fulgens) in china with implications for species conservation. | conservation strategies for the giant panda (ailuropoda melanoleuca) include the development of a self-sustaining ex situ population. this study examined the potential significance of infectious pathogens in giant pandas ex situ. serologic antibody titers against canine distemper virus (cdv), canine parvovirus (cpv), canine adenovirus (cav), canine coronavirus (ccv), canine herpesvirus, canine parainfluenza virus (cpiv), toxoplasma gondii, neospora caninum, and leptospira interrogans were measur ... | 2007 | 18229861 |
| low seroprevalence of neospora caninum infection associated with the limousin breed in cow-calf herds in andorra, europe. | neospora caninum seroprevalence and risk factors affecting seroprevalence in beef cattle in andorra were investigated. antibodies to n. caninum were evaluated by enzyme-linked immunosorbent assay performed on a yearly basis in 1,758 animals older than 6 mo, belonging to 26 herds. mean seroprevalence of antibodies to n. caninum for the herds was 7.4 +/- 1.2% (130/1,758). logistic regression analyses were performed on data from each animal, considering n. caninum seropositivity as the dependent va ... | 2007 | 18163335 |
| neospora caninum and toxoplasma gondii antibodies in dogs from durango city, mexico. | toxoplasma gondii and neospora caninum are structurally similar parasites, with many hosts in common. the prevalence of antibodies to t. gondii and n. caninum was determined in sera from dogs from durango city, mexico. using a modified agglutination test, antibodies to t. gondii were found in 52 (51.5%) of the 101 dogs with titers of 1:25 in 27, 1:50 in 11, 1:100 in 5, 1:200 in 4, 1:400 in 2, 1:800 in 2, and 1:3,200 or higher in 1. antibodies to n. caninum were determined by the indirect immunof ... | 2007 | 18163336 |
| vaccination with microneme protein ncmic4 increases mortality in mice inoculated with neospora caninum. | ncmic4 is a neospora caninum microneme protein that has been isolated and purified on the basis of its unique lactose-binding properties. we have shown that this protein binds to galactosyl residues of lactose; antibodies directed against ncmic4 inhibit host cell interactions in vitro, thus making it a vaccine candidate. because of this feature, ncmic4 was first purified on a larger scale in its native, functionally active form using lactose-agarose affinity chromatography. second, ncmic4 was ex ... | 2007 | 18163338 |
| loss of infectivity of neospora caninum oocysts maintained for a prolonged time. | the purpose of this study was to investigate whether sporulated neospora caninum oocysts, which had been stored for 46 mo in a 2% sulfuric acid solution at 4 degrees c, remain morphologically viable and infective to gerbils (meriones unguiculatus). six gerbils were orally inoculated with doses of 400 or 1,200 oocysts. two mo after inoculation, the animals did not show any clinical signs, had no histological lesions, and were seronegative for n. caninum at 1: 50 in an immunofluorescent antibody t ... | 2007 | 18165712 |
| generation of parasite cysts in cultured cells instead of living animals. | in order to develop any effective means of prevention or therapy against neospora caninum infection, all three life cycle stages of this parasite have to be taken into account. in this project, the in vitro culture of n. caninum bradyzoites containing tissue cysts has been developed, thus allowing to the initiation of stage conversion without the use of animal experimentation. this enables researchers to study novel aspects of stage differentiation, to investigate novel diagnostic antigens, and ... | 2007 | 19835051 |
| current trends in follow-up of trophoblastic function in ruminant species. | during the pregnancy of ruminants, different hormones and proteins are secreted by placenta or corpus luteum allowing the follow up of gestation. among them, progesterone (p4) and pregnancy-associated glycoproteins (pag) were proposed as laboratory tools to establish or to confirm pregnancy diagnosis. in last years, pag assay also provided useful information for researchers working in programs focused on the follow up of trophoblastic function. concentrations of pag appeared as altered after the ... | 2008 | 19261972 |
| review of idiopathic eosinophilic meningitis in dogs and cats, with a detailed description of two recent cases in dogs. | eosinophilic meningoencephalitis (eme) has been described in various species of animals and in humans. in dogs it has been associated with protozoal infections, cuterebral myiasis and various other aetiologies. ten cases of idiopathic eosinophilic meningoencephalitis have been reported in dogs and one in a cat where the origin was uncertain or unknown. the dogs were all males, of various breeds but with a predominance of golden retrievers and rottweilers; they generally had a young age of onset. ... | 2008 | 19496320 |
| [risk factors and prevalence of antibodies anti-neospora caninum in urban and rural dogs from rio grande do sul, brazil]. | serum samples from 339 dogs from rural (beef and dairy farms) and urban areas of municipality of pelotas, rs, were tested by indirect fluorescent antibody test to verify the presence of antibodies anti-neospora caninum. data from dogs took part of an epidemiologic questionnaire to identify possible risk factors associated with the seropositivity. antibodies anti-n. caninum were found 15.6% (53/339) of serum samples, with 5.5% (6/109) of positive dogs from urban area and 20.4% (47/230) from rural ... | 2008 | 20059865 |
| [determination and correlation of anti-neospora caninum antibodies in cattle and dogs from paraná, brazil]. | the protozoan parasite neospora caninum is a major cause of abortion in cattle worldwide. the aim of the present study was to determine and to compare the presence of anti-neospora caninum antibodies in cattle, as well as in farm dogs, and the relationship among them. the research was conducted in different mesoregions of paraná state, southern brazil. sera from 1.263 cattle, from 77 farms were tested for antibodies to n. caninum by elisa. sera from 129 dogs, from 35 farms were tested for antibo ... | 2008 | 20059847 |
| [seroprevalence of antibodies anti-neospora caninum and anti-toxoplasma gondii in captive wild canids]. | neosporosis is considered one of the main cause of abortion in dairy cattle in the world. the prevalence of neospora caninum in wild species has been studied since the coyote (canis latrans), a north american wild canid specie was discovered as definitive host of this parasite. the aim of the present study was to determine the serum prevalence of n. caninum and t. gondii in wild native canids species from brazil. serum samples of 25 crab-eating dogs (cerdocyon thous), five pampas fox (lycalopex ... | 2008 | 20059860 |
| the differential protein expression profiles and immunogenicity of tachyzoites and bradyzoites of in vitro cultured neospora caninum. | we report a study on the variations in the protein expression profiles of tachyzoites and bradyzoites of neospora caninum. the in vitro stage conversion of n. caninum-infected vero cells was induced by continuous treatment of infected cultures with 70 mum sodium nitroprusside (snp) for up to 9 days. the stage conversion indicated by the expression of the bradyzoite-specific antigen bag1 was analyzed by immunofluoresence assay. morphological changes between tachyzoites and bradyzoites and localiz ... | 2008 | 18597117 |
| [occurrence of antibodies anti-neospora caninum in dogs of botucatu range micro region, state of são paulo, brazil]. | neosporosis is a parasitic disease caused by a protozoon neospora caninum, which is recognized as an important cause of bovine abortion and canine neuropathy. considering the isolation of n. caninum and the frequent serology in bovines of our region, the objectives of the present trial were to evaluate the occurrence of antibodies anti-n. caninum in dogs of the botucatu range micro region, in the state of são paulo, and its association with gender, age and origin (urban area--exclusively from th ... | 2008 | 18554432 |
| neospora caninum antibodies in wild carnivores from spain. | serum samples from 251 wild carnivores from different regions of spain were tested for antibodies to neospora caninum by the commercial competitive screening enzyme linked immunosorbent assay (c-elisa) and confirmed by neospora agglutination test (nat) and/or by indirect fluorescent antibody test (ifat). samples with antibodies detected by at least two serological tests were considered seropositive. antibodies to n. caninum were found in 3.2% of 95 red foxes (vulpes vulpes); in 21.4% of 28 wolve ... | 2008 | 18556128 |
| serological survey of neospora caninum infection among dogs in japan through species-specific elisa. | a seroepidemiological survey of neospora caninum infection among dogs in japan was conducted using species-specific enzyme-linked immunosorbent assay with recombinant surface antigen (nc-sag1t). among 1,206 dogs examined, 126 dogs (10.4%) from 30 prefectures from hokkaido to okinawa were positive to n. caninum infections, which were more frequently detected in females than males. siberian huskies showed the highest positive rate compared with the other breeds. dogs with pyometra and diabetes mel ... | 2008 | 18772569 |
| a four year longitudinal sero-epidemiology study of neospora caninum in adult cattle from 114 cattle herds in south west england: associations with age, herd and dam-offspring pairs. | neosporosis caused by the protozoan parasite neospora caninum, is an economically important cause of abortion, stillbirth, low milk yield, reduced weight gain and premature culling in cattle. consequently, a seroepidemiological study of n. caninum antibodies was conducted in england with 29,782 samples of blood taken from 15,736 cattle from 114 herds visited on three occasions at yearly intervals. herds were categorised into lower (< 10%) and higher (> or = 10%) median herd seroprevalence. hiera ... | 2008 | 18793402 |
| the role of neospora caninum and toxoplasma gondii in spontaneous bovine abortion in argentina. | the aim of the present work was to evaluate the role of neospora caninum and toxoplasma gondii infections in spontaneous bovine abortions in argentina. based on histopathological results, 70 presumptive cases of apicomplexan protozoal abortion from a total of 666 cases of spontaneous bovine abortion submitted to the national institute of agrarian technology, balcarce, from 1999 to 2007 were included in this study. n. caninum infection was diagnosed by an indirect fluorescent antibody test (ifat) ... | 2008 | 18691819 |
| toxoplasma rhoptries: unique secretory organelles and source of promising vaccine proteins for immunoprevention of toxoplasmosis. | toxoplasma gondii is an obligate intracellular protozoan parasite classified in the phylum apicomplexa, which includes numerous notable human and animal pathogens (plasmodium species, cryptosporidium species, neospora caninum, etc.). the invasive stages of apicomplexans are characterized by the presence of an apical complex composed of specialized cytoskeletal and secretory organelles, including rhoptries. rhoptries, unique apical secretory organelles shared exclusively by all apicomplexan paras ... | 2008 | 18670609 |
| encephalitis in aborted bovine fetuses associated with bovine herpesvirus 1 infection. | brain tissue from 12 aborted bovine fetuses submitted to the wisconsin veterinary diagnostic laboratory revealed histologic lesions that consisted of glial nodules and variable degrees of mononuclear inflammation, microhemorrhage, neuronal necrosis, and cerebral cortical cavitation. a diagnosis of bovine herpesvirus 1 (bhv-1) abortion had been made in all of these cases through multiple testing modalities. brain tissue from 8 of the 12 fetuses was immunohistochemically stained with a monoclonal ... | 2008 | 18460615 |
| generation and infection of bovine pbmc-derived dendritic cells with neospora caninum. | 2008 | 18683066 | |
| serological survey of toxoplasma gondii in wild waterfowl in chukotka, kamchatka, russia and hokkaido, japan. | antibodies to toxoplasma gondii were assayed by elisa in 22 experimentally inoculated domestic ducks. in addition, a serological assay was carried out at obihiro, hokkaido, japan, in 2004 and 2005, on 221 wild ducks of 5 species: anas platyrhynchus (n = 111); a. poecilorhyncha (n = 27); a. acuta (n = 58); a. penelope (n = 16); and a. crecca (n = 9). assays were also conducted using sera from 197 wild geese of 2 species, i.e., anser albifrons (n = 162) and ans. fabalis (n = 35). birds were collec ... | 2008 | 18576786 |
| occurrence of antibodies to neospora caninum and toxoplasma gondii in dogs from pernambuco, northeast brazil. | a serological survey was carried out to assess the occurrence of anti-neospora caninum antibodies in dogs from the state of pernambuco. a total of 625 serum samples of dogs (289 from paulista, 168 from amaraji and 168 from garanhuns) were tested by an immunofluorescence antibody assay for the detection of anti-n. caninum antibodies. a total of 177 (28.3%; ic 95%, 24.9-32.1) samples were positive. the seropositivity rates found in paulista, amaraji and garanhuns were 26% (ic 95%, 21-31.4), 26.2% ... | 2008 | 18723288 |
| toxoplasma gondii and neospora caninum antibodies in dogs from grenada, west indies. | toxoplasma gondii and neospora caninum are structurally similar parasites with many common hosts. the prevalence of antibodies to t. gondii and n. caninum was determined in sera from dogs in grenada, west indies. using a modified agglutination test, antibodies to t. gondii were found in 52 (48.5%) of the 107 dogs, with titers of 1:25 in 17, 1:50 in 19, 1:100 in 7, 1:1,600 in 5, and 1:3,200 or higher in 4. seroprevalence increased with age from 2.2% in dogs <6 mo old to 18.9% in dogs older than 2 ... | 2008 | 18605807 |
| diagnosis of sarcocystis cruzi, neospora caninum, and toxoplasma gondii infections in cattle. | the aim of the study was to diagnose sarcocystis sp. infections in cattle and to detect coinfections by toxoplasma gondii and/or neospora caninum. blood, diaphragm, esophagus, and myocardium from 90 beef cattle from argentina were collected. histopathological, immunohistochemical, polymerase chain reaction assays, and direct microscopical examination were carried out. sarcocysts from myocardium were measured and counted. indirect fluorescent antibody test (ifat) for the three protozoans was perf ... | 2008 | 18066600 |
| bovine immune response to inoculation with neospora caninum surface antigen srs2 lipopeptides mimics immune response to infection with live parasites. | infection of cattle with neospora caninum protozoa, the causative agent of bovine protozoal abortion, results in robust cellular and humoral immune responses, particularly cd4(+) t-lymphocyte activation and gamma interferon (ifn-gamma) secretion. in the present study, n. caninum srs2 (ncsrs2) t-lymphocyte-epitope-bearing subunits were incorporated into dna and peptide preparations to assess cd4(+) cell proliferation and ifn-gamma t-lymphocyte-secretion immune responses in cattle with predetermin ... | 2008 | 18305105 |
| neospora caninum infection in greek dairy cattle herds detected by two antibody assays in individual milk samples. | a survey to demonstrate the presence and the extent of neospora caninum infection in dairy cattle was carried out in greece. seven hundred and seventy-seven (777) individual milk samples from all milking cows in 10 dairy herds were tested using an elisa (p38-milk-elisa) and immunoblot analysis. the herd prevalence was 80.0% and 100.0%, while the overall prevalence was 15.2% (ranging between 0.0% and 38.9%) and 27.9% (ranging between 3.4% and 61.6%) based on the results of the elisa and the immun ... | 2008 | 18158217 |