Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| the effect of the dam-calf relationship on serostatus to neospora caninum on 20 costa rican dairy farms. | an epidemiological study was conducted on 20 dairy herds previously diagnosed as seropositive for neospora caninum. the number of females per farm varies from 41 to 296. all females present on the farms were bled once in the period of july and august 2000. a total of 3002 females were bled. an indirect elisa was used to determine the serostatus of the animals. the analysis of the data was performed in four steps: (1) descriptive statistics about the serological status and general characteristics ... | 2003 | 12788252 |
| electron microscopic study of in vitro cultures of hammondia heydorni (berlin 1996) tachyzoites after passages through dogs, mice, rats, guinea pigs and jirds. | this study describes the fine structure and reproduction of tachyzoites of hammondia heydorni (isolate berlin 1996) after in vitro cultivation of stages isolated from serial passages between dogs and various intermediate hosts such as mice, rats, guinea pigs, jirds, knock-out mice, goats and sheep. the tachyzoites are identical with respect to their ultrastructural features to the corresponding stages of the nc-1-strain of neospora caninum. this fact conforms with other already published data an ... | 2003 | 12838414 |
| serological evidence of neospora caninum infections in beef bulls in six counties of the corrientes province, argentina. | the purpose of the present work was to identify neospora caninum infections in beef bulls belonging to 19 herds from six counties located in the corrientes province, argentina. the presence of antibodies to n. caninum was evaluated in 305 serum samples of bulls (bos taurus and bos indicus). age and breed were recorded. an indirect fluorescent antibody test was used to determine specific antibodies. the number of bulls with natural neospora-infection was 15 of 305 (4.9%). no association between s ... | 2003 | 12809751 |
| prevalence of antibodies to neospora caninum in dogs from amazon, brazil. | neospora caninum is an important cause of abortion in dairy cattle worldwide. dogs are important in the epidemiology of this parasite because they are the only hosts known to excrete n. caninum oocysts. antibodies to n. caninum were assayed in serum samples from 157 dogs from monte negro, rondônia, amazon, brazil using the indirect fluorescent antibody test. antibodies to n. caninum were found in 13 (8.3%) of dogs in titers of 1:50 in 1, 1:100 in 2, 1:200 in 5, 1:800 in 1, 1:1600 in 2, and 1:320 ... | 2003 | 12860070 |
| antibodies to neospora caninum in european brown hare (lepus europaeus). | neospora caninum antibodies in european brown hares (lepus europaeus) were reported for the first time in sera collected from hares imported in italy from east europe. sera from 93 hares coming from hungary and 44 hares coming from slovakia were tested using a direct agglutination test (dat). seroprevalence was 8.0% at the 1:40 dilution and 1.5% at 1:320 dilution. seroprevalence in females and males and in different countries of origin did not differ significantly. our results indicate that euro ... | 2003 | 12860071 |
| first pcr-confirmed report of a neospora caninum-associated bovine abortion in austria. | 2003 | 12723631 | |
| vaccination of ewes for prevention of vertical transmission of neospora caninum. | to evaluate the immunologic response of a killed tachyzoite vaccine against neospora caninum and its effectiveness in preventing vertical transmission of n caninum in sheep. | 2003 | 12693535 |
| characterization of an alpha tubulin gene sequence from neospora caninum and hammondia heydorni, and their comparison to homologous genes from apicomplexa. | the gene coding for a tubulin has been isolated by the polymerase chain reaction and sequenced from 2 isolates of neospora caninum (nc-liverpool and nc-sweb1). the data show that the gene, as in toxoplasma gondii, is single copy and contains 3 exons and 2 introns and is identical in sequence in the 2 isolates studied. comparison of the predicted protein sequence shows it to be identical to the a tubulin protein encoded by the t. gondii gene. the majority of the nucleotide substitutions that have ... | 2003 | 12866794 |
| detection of hammondia heydorni-like organisms and their differentiation from neospora caninum using random-amplified polymorphic dna-polymerase chain reaction. | neospora caninum and hammondia heydorni are morphologically and phylogenetically related coccidians that are found in dogs. new diagnostic genetic loci, based on random-amplified polymorphic dna-polymerase chain reaction (rapd-pcr), were developed to aid in the detection of h. heydorni-like parasites and to discriminate them from n. caninum and other related coccidians of dogs. on the basis of the data obtained from 5 random decamers, h. heydorni (manhattan-1) and n. caninum (nc1) were character ... | 2003 | 14627168 |
| neospora caninum antigens recognized by mouse igg at different stages of infection including recrudescence. | western blotting was performed to analyze neospora caninum tachyzoite antigens recognized by mouse igg at different stages of infection including recrudescence. at the early stage of infection, a 36-38 kda antigen was clearly recognized by the mouse antisera. after day 48 postinoculation, the signal of the 36-38 kda antigen gradually weakened. meanwhile, a 43 kda antigen was intensely and continuously recognized from 48 to 125 days postinoculation. this 43 kda antigen was clearly detectable with ... | 2003 | 12867739 |
| immunization of mice with plasmid dna coding for ncgra7 or ncshsp33 confers partial protection against vertical transmission of neospora caninum. | the purpose of the present study was to use direct plasmid deoxyribonucleic acid (dna) injection to identify specific antigens that confer protection against congenital transfer of neospora caninum. inbred balb/c mice were vaccinated before pregnancy with a recombinant plasmid containing sequences encoding n. caninum antigen ncgra7 or ncshsp33. the mice were challenged with n. caninum tachyzoites at 10-12 days of gestation. whereas 100% of pups born from dams immunized with control plasmid conta ... | 2003 | 12880248 |
| hammondia heydorni from the arabian mountain gazelle and red fox in saudi arabia. | unsporulated oocysts were detected in the feces of an arabian red fox (vulpes vulpes arabica) between 6 and 8 days after it had been fed meat from arabian mountain gazelles (gazella gazella) known to contain sarcocysts. no oocysts were discovered in the feces of other experimental cubs, although sporocysts of sarcocystis spp. were passed subsequently by all cubs that were fed gazelle meat, including those fed with reem (g. subgutturosa marica). the oocysts sporulated in 3 days at room temperatur ... | 2003 | 12880254 |
| protracted shedding of oocysts of neospora caninum by a naturally infected foxhound. | feces from 15 dogs at 2 different foxhound kennels in the u.k. were examined microscopically for the presence of oocysts of neospora caninum. one sample containing approximately 400 candidate oocysts per gram was positive in a polymerase chain reaction (pcr) using n. caninum-specific primers. in a sample taken 4 mo later from the same hound. n. caninum oocysts were again detected visually and by pcr. this is the third reported case of a dog naturally excreting oocysts of n. caninum and suggests ... | 2003 | 12880273 |
| the pathogenesis of neosporosis in pregnant cattle: inoculation at mid-gestation. | to investigate the pathogenesis of bovine neosporosis, 14 pregnant cattle were each inoculated subcutaneously with either 10(7) or 5 x 10(8) neospora caninum (strain nc1) tachyzoites at 140 days' gestation. serial necropsies were then carried out over an 8-week period. in the placenta, neospora dna and histopathological changes were observed in samples taken 14 days post-inoculation (dpi), with focal necrosis of maternal caruncular septa and fetal placental villi, serum leakage, and a maternal a ... | 2003 | 12921725 |
| seroprevalence of neospora caninum in aborting dairy cattle in the czech republic. | a serological survey for antibodies against neospora caninum in aborting cattle was carried out in the czech republic. serum samples from 463 aborting dairy cows originated from 137 farms from different parts of the czech republic were tested for presence of n. caninum antibodies by use of an enzyme-linked immunosorbent assay (elisa) and an indirect fluorescent antibody test (ifat). antibodies (> or = 1:640) to n. caninum were found in 18 (3.9%) of 463 aborting cows. farm prevalence in aborting ... | 2003 | 12935739 |
| development and evaluation of a seminested pcr for detection and differentiation of babesia gibsoni (asian genotype) and b. canis dna in canine blood samples. | canine babesiosis has recently been recognized as an emerging infectious disease of dogs in north america. we sought to develop a seminested pcr to detect and differentiate babesia gibsoni (asian genotype), b. canis subsp. vogeli, b. canis subsp. canis, and b. canis subsp. rossi dna in canine blood samples. an outer primer pair was designed to amplify an approximately 340-bp fragment of the 18s rrna genes from b. gibsoni (asian genotype), b. canis subsp. vogeli, b. canis subsp. rossi, and b. can ... | 2003 | 12958243 |
| laboratory passage and characterization of an isolate of toxoplasma gondii from an ocular patient in korea. | toxoplasma gondii tachyzoites were isolated from the blood of an ocular patient, and have been successfully passaged in the laboratory, for over a year, by peritoneal inoculation in mice. the isolated parasite was designated the korean isolate-1 (ki-1) and its characteristics were compared with those of the rh strain, a wellknown virulent strain originating from a child who suffered from encephalitis. the morphology, pathogenicity, infectivity and cell culture characteristics of the ki-1 were si ... | 2003 | 12972728 |
| elisa detection of igg antibody against a recombinant major surface antigen (nc-p43) fragment of neospora caninum in bovine sera. | an elisa was established to measure bovine igg directed against the recombinant antigenic determinant of nc-p43, a major surface antigen of neospora caninum. in a previous study, two thirds of the c-terminal of the molecule was expressed as a 6 x his tagged protein (ncp43p) for elisa using 2/3 of the n-terminal of sag1 from toxoplasma gondii as a control (tgsag1a). among 852 cattle sera collected from stock farms scattered nation-wide, 103 sera (12.1%) were found to react with ncp43p positively, ... | 2003 | 12972732 |
| first demonstration of protective immunity against foetopathy in cattle with latent neospora caninum infection. | the parasite neospora caninum is an important cause of abortion in cattle world-wide. chronically infected dams transmit the parasite transplacentally and infected foetuses may be aborted or born chronically infected but clinically normal. chronically infected cows repeatedly transmit the parasite to foetuses in several pregnancies and some may abort more than once suggesting that the immune response in these cattle is compromised during pregnancy. to investigate the nature of the immune respons ... | 2003 | 13129528 |
| aetiology of bovine abortion in argentina. | necropsies were performed on 354 fetuses from dairy and beef herds submitted from 1994 to 2000 to the diagnostic laboratories at instituto nacional de tecnología agropecuaria, balcarce, argentina. samples from the fetuses were examined for pathogenic organisms and processed for microscopic examination. an aetiological diagnosis was made for 161 (45.5%) of the fetuses. no diagnosis was made for 193 (54.5%) fetuses. infectious agents were isolated from 122 (34.4%) of the fetuses, bacterial agents ... | 2003 | 14509450 |
| anti-protozoal efficacy of medicinal herb extracts against toxoplasma gondii and neospora caninum. | the purpose of this study was to determine whether alcohol extracts of herbs (sophora flavescens aiton, sinomenium acutum (thunb.) rehder and e.h. wilson, pulsatilla koreana (yabe ex nakai) nakai ex t. mori, ulmus macrocarpa hance and torilis japonica (houtt.) dc.) from south korea, possess in vitro anti-protozoal activity against cultures of toxoplasma gondii and neospora caninum. these herbs have been used as human anti-parasitics in asian countries for many years. alcohol extracts of these he ... | 2003 | 14519322 |
| dermatitis in a dog associated with an unidentified toxoplasma gondii-like parasite. | protozoal dermatitis was diagnosed in a 6-year-old female great dane dog from rio de janeiro, brazil. the dog died because of a chronic illness with an ehrlichia-like organism. numerous apicomplexan parasites were identified histologically in the section of dermal lesions. the protozoan reacted with toxoplasma gondii polyclonal rabbit serum but not with neospora caninum or sarcocystis neurona antibodies. ultrastructurally, the protozoa was not t. gondii because it had schizont-like structures wi ... | 2003 | 14519327 |
| cytokine gene expression in dams and foetuses after experimental neospora caninum infection of heifers at 110 days of gestation. | neospora caninum is a major cause of abortion in cattle. an essential role for th1 cytokines, such as ifn-gamma and il-12 in protective immunity against n. caninum in murine models has been indicated. however, little is known about immunity to neospora in pregnant cattle where a considerable level of immunomodulation may exist. in this study, the immune response of heifers infected early in the second trimester of pregnancy by intravenous inoculation of n. caninum tachyzoites was compared with i ... | 2003 | 14521581 |
| prevalence of antibodies to neospora caninum, sarcocystis neurona, and toxoplasma gondii in wild horses from central wyoming. | sarcocystis neurona, neospora caninum, n. hughesi, and toxoplasma gondii are 4 related coccidians considered to be associated with encephalomyelitis in horses. the source of infection for n. hughesi is unknown, whereas opossums, dogs, and cats are the definitive hosts for s. neurona, n. caninum, and t. gondii, respectively. seroprevalence of these coccidians in 276 wild horses from central wyoming outside the known range of the opossum (didelphis virginiana) was determined. antibodies to t. gond ... | 2003 | 14533680 |
| meningoencephalitis associated with an unidentified apicomplexan protozoan in a pacific harbor seal. | a pacific harbor seal (phoca vitulina richardsii) was found on the central california coast with neurologic signs and labored breathing, which were unresponsive to treatment. necropsy revealed a nonsuppurative necrotizing meningoencephalitis, a multilocular thymic cyst, and nonsuppurative cystitis and renal pyelitis. microscopic examination revealed protozoans in the brain, thymic cyst, and bladder mucosa. ultrastructurally, the protozoal tachyzoites were different from those of neospora caninum ... | 2003 | 14533706 |
| autofluorescence of toxoplasma gondii and related coccidian oocysts. | this is the first report of blue autofluorescence as a useful characteristic in the microscopic detection of toxoplasma gondii, hammondia hammondi, hammondia heydorni, neospora caninum, besnoitia darlingi, and sarcocystis neurona oocysts or sporocysts. this autofluorescence is of sufficient intensity and duration to allow identification of these oocysts from complex microscopic sample backgrounds. as with the autofluorescence of related coccidia, the oocysts glow pale blue when illuminated with ... | 2003 | 14533708 |
| seroprevalence of neospora caninum and toxoplasma gondii antibodies in the south american opossum (didelphis marsupialis) from the city of são paulo, brazil. | antibodies to neospora caninum and toxoplasma gondii were assayed in sera of 396 opossums (didelphis marsupialis) from the city of são paulo, brazil. antibodies to n. caninum were assayed using the indirect immunofluorescent antibody test (ifat). antibodies (ifat, approximately 1:25) to n. caninum were found in 84 opossums (d. marsupialis) in titers of 1:25 in 46, 1:50 in 20, 1:100 in 17, and 1:400 in 1. antibodies to t. gondii were assayed with the modified agglutination test (mat) and the ifat ... | 2003 | 14533710 |
| isolation of neospora caninum from a blind calf in paraná, southern brazil. | 2003 | 14533770 | |
| neospora caninum and toxoplasma gondii: a novel adhesion/invasion assay reveals distinct differences in tachyzoite-host cell interactions. | this paper describes an adhesion/invasion assay, based on combined pyrrolidine dithiocarbamate (pdtc) and antibody treatment of parasites followed by quantitative real-time pcr. this pdtc-pcr assay can be used to comparatively assess the participation of host cell- and parasite-associated components during host cell adhesion and entry by neospora caninum and toxoplasma gondii tachyzoites, respectively, and is potentially applicable to any other apicomplexan parasite. the assay allows to determin ... | 2003 | 14552862 |
| a role for balance of interferon-gamma and interleukin-4 production in protective immunity against neospora caninum infection. | a suitable balance in the production of th1/th2-type cytokines has a crucial role in the control of microbial infections. we investigated cytokine production patterns and effects during neospora caninum infection, based on two mouse models and an in vitro system. in the acute infection of n. caninum, balb/c-background ifn-gamma-deficient mice that were sensitive to the n. caninum infection showed high levels of il-10 production, whereas significant levels of interferon-gamma (ifn-gamma) and inte ... | 2003 | 14559161 |
| humoral immune response to infectious agents in aborted bovine fetuses in argentina. | the purpose of this study was to characterize the exposure of bovine aborted fetuses from beef and dairy herds of the humid pampas of argentina to different infectious agents by the evaluation of fetal fluid antibodies. presence of fetal antibodies to bovine viral diarrhea virus genotype 1 (bvdv-1), bovine herpes virus type 1 (bhv-1), leptospira interrogans, brucella abortus, and neospora caninum was determined. of the 95 fetuses processed, 66 came from 49 beef herds and 29 from 12 dairy herds. ... | 2003 | 14587376 |
| toxoplasma gondii, neospora caninum, sarcocystis neurona, and sarcocystis canis-like infections in marine mammals. | toxoplasma gondii, neospora caninum, sarcocystis neurona, and s. canis are related protozoans that can cause mortality in many species of domestic and wild animals. recently, t. gondii and s. neurona were recognized to cause encephalitis in marine mammals. as yet, there is no report of natural exposure of n. caninum in marine mammals. in the present study, antibodies to t. gondii and n. caninum were assayed in sera of several species of marine mammals. for t. gondii, sera were diluted 1:25, 1:50 ... | 2003 | 14580799 |
| seroprevalence of neospora caninum and toxoplasma gondii in captive and free-ranging nondomestic felids in the united states. | seven serum samples of 101 samples from nondomestic, captive and free-ranging felids from the united states were indirect fluorescent antibody positive for antibodies to neospora caninum, whereas 44 samples were positive for antibodies to t. gondii. although none of the captive animals displayed clinical signs of disease, nondomestic felids in the united states have been exposed to, and are likely infected with, n. caninum and t. gondii. this may have serious implications for zoological gardens ... | 2003 | 14582785 |
| reproductive losses due to neospora caninum in a beef herd in argentina. | reproductive losses in a beef herd of 857 heifers with a pregnancy rate of 86.3% are described. after pregnancy testing, 69 abortions were seen during a 3 month period. before calving season, three heifers had delivered pre-mature non-viable calves. serum samples from 58 of 69 aborted heifers were available for serological tests. in order to compare the seroprevalence in non-aborted vs. aborted heifers, 214 pregnant animals were bleed during the abortion storm. in addition, blood samples were co ... | 2003 | 14629003 |
| assessment of antibody avidity in aborting cattle by a somatic neospora caninum tachyzoite antigen igg avidity elisa. | a neospora caninum igg avidity elisa was carried out on the basis of a somatic n. caninum tachyzoite antigen. the test was validated using experimentally infected calves, where a clear maturation of the igg avidity over time could be demonstrated. at a maximum of 82 days after infection (d.p.i.), all animals showed antibody avidities ranging above 35%, and respective sera were thus defined as highly avid. sera of 103 naturally infected seropositive cows with abortion (n. caninum association was ... | 2003 | 12581580 |
| validation of a neospora caninum iscom elisa without a gold standard. | neospora caninum is an intracellular parasite which causes abortion in cattle worldwide. one problem in the validation of the different methods for demonstration of this parasite is the lack of an appropriate gold standard. to validate an immunostimulating complex (iscom) enzyme-linked immunoassay (elisa) used to detect antibodies to n. caninum, sera from 244 cattle in five swedish dairy herds infected with n. caninum were analysed. the sera also were analysed by a standard indirect-fluorescent ... | 2003 | 12581597 |
| evaluation of a single serological screening of dairy herds for neospora caninum antibodies. | twenty-one dairy herds with a history of neospora caninum-associated abortions were used for a longitudinal serological study. a total of 1,676 animals were blood sampled 3 times and used to evaluate a single serological screening for n. caninum antibodies. the results of the first serological screening were compared with the results based on three consecutive samples, whereby two or more positive or negative test results per animal were considered to determine its serological status as positive ... | 2003 | 12482645 |
| neospora caninum: pregnancy, parasites and prolactin. | 2003 | 12488220 | |
| application of the neospora caninum igg avidity elisa in assessment of chronic reproductive losses after an outbreak of neosporosis in a herd of beef cattle. | point-source infections are most likely the cause for neospora caninum-induced abortion outbreaks in cattle, whereas an increased annual abortion rate may be a consequence of vertical transmission. the aims of the present study were to examine the reproductive effects of neosporosis in a beef herd for 3 years, after a point-source outbreak and to use igg avidity serology to examine the chronicity of infections and patterns of transmission. during the study, 76-78% of animals were seropositive fo ... | 2003 | 12580287 |
| evaluation by different diagnostic techniques of bovine abortion associated with neospora caninum in spain. | eighty foetuses from some of the main cattle-producing regions in spain were analysed to investigate the participation of neospora caninum in cases of bovine abortion. diagnosis of the infection was determined by histopathological analysis complemented with immunohistochemistry, serology (ifat and elisa) and pcr tests. a total of 38.8% of the bovine foetuses analysed were considered to be infected by at least one of the diagnostic techniques used. microscopic lesions consistent with neospora inf ... | 2003 | 12531290 |
| analysis of the sarcocystis neurona microneme protein snmic10: protein characteristics and expression during intracellular development. | sarcocystis neurona, an apicomplexan parasite, is the primary causative agent of equine protozoal myeloencephalitis. like other members of the apicomplexa, s. neurona zoites possess secretory organelles that contain proteins necessary for host cell invasion and intracellular survival. from a collection of s. neurona expressed sequence tags, we identified a sequence encoding a putative microneme protein based on similarity to toxoplasma gondii mic10 (tgmic10). pairwise sequence alignments of snmi ... | 2003 | 12814647 |
| gene discovery in the apicomplexa as revealed by est sequencing and assembly of a comparative gene database. | large-scale est sequencing projects for several important parasites within the phylum apicomplexa were undertaken for the purpose of gene discovery. included were several parasites of medical importance (plasmodium falciparum, toxoplasma gondii) and others of veterinary importance (eimeria tenella, sarcocystis neurona, and neospora caninum). a total of 55192 ests, deposited into dbest/genbank, were included in the analyses. the resulting sequences have been clustered into nonredundant gene assem ... | 2003 | 12618375 |
| vaccination of mice against experimental neospora caninum infection using ncsag1- and ncsrs2-based recombinant antigens and dna vaccines. | ncsag1 and ncsrs2, the two major immunodominant tachyzoite surface antigens of the apicomplexan parasite neospora caninum, were investigated for their potential as vaccine candidates in mice. recombinant recncsrs2 and recncsag1 were expressed in escherichia coli as poly-histidine-tagged fusion proteins. separate groups of mice were immunized with purified recncsag1, recncsrs2, or a combination of both, and were then challenged with n. caninum tachyzoites. subsequent experiments included intramus ... | 2003 | 12741509 |
| evaluation of an inactivated neospora caninum vaccine in beef feedlot steers. | to evaluate effects of vaccination of feedlot steers against bovine neosporosis on weight gain, feed intake and efficiency (feed intake per gain), and carcass characteristics. | 2003 | 12619843 |
| abortion epidemic in a dairy herd associated with horizontally transmitted neospora caninum infection. | a dairy herd experienced an abortion epidemic during which 43 per cent of the cows at risk aborted. neospora caninum infection was demonstrated in four of six fetuses suitable for examination and the group of at-risk cows that aborted had significantly higher n. caninum antibody concentrations than the at-risk cows that delivered a live calf at term (p<0.001). the antibody concentrations in the cow herd were significantly higher than in the youngstock (p<0.001), and the concentrations in the you ... | 2003 | 12620035 |
| determination and correlation of anti-neospora caninum antibodies in dogs and cattle from mexico. | the aim of the present study was to determine and to compare through an indirect enzyme linked immunosorbent assay (elisa) test, the presence of anti-neospora caninum antibodies in city and farm dogs, as well as in farm cows, and the relationship among them. the correlation between anti-n. caninum antibodies in farm dogs and cattle was also assessed. the research was conducted in the dairy region of tizayuca, hidalgo, mexico. the frequency of anti-n. caninum antibodies was significantly higher i ... | 2003 | 12760481 |
| anti-parasitic activity of depudecin on neospora caninum via the inhibition of histone deacetylase. | neospora caninum is an apicomplexan parasite associated with abortion in cattle worldwide. apicidin, histone deacetylase (hdac) inhibitor, has shown a broad spectrum of anti-protozoal activity against apicomplexan parasites. cultured vero cells infected with n. caninum tachyzoites were treated with 0.5 microg/ml of depudecin, another known natural product isolated from altermaria brassiciicola with anti-histone deacetylase activity, to examine the efficacy of depudecin against intracellular mult ... | 2003 | 12623206 |
| characterisation of the first australian isolate of neospora caninum from cattle. | to isolate neospora caninum from a congenitally infected calf. | 2002 | 12465814 |
| characterization of neospora caninum protease, ncsub1 (nc-p65), with rabbit anti-n54. | ncsub1 (formerly known as nc-p65) is the first molecularly described proteolytic enzyme of the intracellular protozoan parasite neospora caninum. this report describes the characterization of a rabbit anti-n54, which is an antiserum generated against an internal fragment of ncsub1 (amino acids 649-783). in immunofluorescence studies rabbit and-n54 labeled the apical end of the fixed parasite. by immuno-gold electron microscopy, the antibody bound primarily to the microneme organelles of the para ... | 2002 | 12537103 |
| survey of neospora caninum antibodies in dairy and beef cattle from five regions of the united states. | serum samples from 4,907 cows from 93 dairy and five beef herds were analyzed for antibodies to neospora caninum by commercially available elisa. the herds were recruited from 20 states and the territory of puerto rico. a questionnaire assessed the herd's abortion history and exposure risks to n. caninum. results showed that 788 (16%) cows tested positive for antibodies to n. caninum. at least one positive animal was detected in 90% of the herds tested. the prevalence of cows seropositive to n. ... | 2002 | 12584676 |
| neospora caninum in vitro: evidence that the destiny of a parasitophorous vacuole depends on the phenotype of the progenitor zoite. | we previously reported that neospora caninum can be induced to express bagi, a bradyzoite antigen, within 3 days of culture under stress conditions. the main goals of the present experiment were to increase the expression of bagi in vitro (in part by extending cultures for 9 days), to observe parasitophorous vacuoles at various points of stage differentiation, and to test the ability of organisms produced in vitro to function like mature bradyzoites. expression of bag1 and of a tachyzoite antige ... | 2002 | 12537100 |
| isolation of viable toxoplasma gondii from naturally infected aborted bovine fetuses. | neospora caninum and toxoplasma gondii are related parasites. the former is a common cause of abortion in dairy cattle. the latter has not been conclusively demonstrated in bovine fetuses. during the course of attempts to isolate n. caninum from aborted fetuses, t. gondii was isolated from 2 aborted fetuses, 1 from portugal and 1 from the united states. both isolates were made by bioassay of fetal brains in mice. the fetus from portugal was about 5 mo in gestational age, and the fetus from the u ... | 2002 | 12537120 |
| prevalence of antibodies to neospora caninum and sarcocystis neurona in sera of domestic cats from brazil. | parasite biology, epidemiology and systematics laboratory, animal and natural resources institute, agricultural research service, united states department of agriculture, building 1001, beltsville, maryland 20705-2350 antibodies to neospora caninum and sarcocystis neurona were determined in serum samples of 502 domestic cats from brazil using direct agglutination tests with the respective antigens. antibodies to s. neurona were not found in 1:50 dilution of any serum in the s. neurona agglutinat ... | 2002 | 12537122 |
| risk of abortion associated with neospora caninum during different lactations and evidence of congenital transmission in dairy cows. | to examine the relationship between exposure to neospora caninum and abortion in dairy cows during their first, second, third, and fourth or later lactations and to establish the main mode of transmission in female calves from birth until their first pregnancy was terminated by abortion or parturition. | 2002 | 12494973 |
| the influence of feeding and maintenance system on occurrence of toxoplasma gondii infections in dogs. | serum samples of 113 dogs visiting "outpatient clinics", 52 dogs kept in shelters and 35 animals from a military dog training centre were examined for toxoplasma gondii specific antibodies using a latex agglutination test. significant differences in seroprevalences were found between dogs from the training centre (8.6% of positive results) and the other populations examined (40.7% of positive seroreagents in animals visiting outpatient clinics and 44.2% in the group from shelters, respectively). ... | 2002 | 12512556 |
| diagnostic survey of bovine abortion in korea: with special emphasis on neospora caninum. | causes of bovine abortion were surveyed in korea within a designated period from the cases submitted to the department of veterinary pathology, college of veterinary medicine, seoul national university. one hundred and eighty aborted fetuses and maternal sera were evaluated by necropsy, histopathology, bacteriology, virology, pcr, and serologic tests. the causes of abortion were identified in 108 (60%) cases, of which 38 (21.1%) were due to the infection with neospora caninum. none of the 38 cas ... | 2002 | 12520105 |
| neospora caninum: oocyst challenge of pregnant cows. | three pregnant cows were each orally challenged at 10 weeks of gestation with 600 sporulated oocysts of neospora caninum. the number of oocysts was limited by those available. in concurrent bioassays, one oocyst per os infected each of two gerbils. challenged cattle developed neospora-specific antibody, cell proliferation and gamma-interferon responses. n. caninum specific pcr demonstrated persisting infection in the brains of cows 4 months after calving. abortion was not induced and there was n ... | 2002 | 12383634 |
| seroprevalence of antibodies to neospora caninum in dogs from spain. | the prevalence of neospora caninum antibodies was determined in sera of 139 dogs from catalonia (northeastern spain) using the indirect immunofluorescence antibody test (ifat). antibodies in the ifat were found in 17 of 139 dogs (12.2%) with titers ranging from 1:50 to 1: 1,600. seroprevalence was higher in dogs over 1 yr old compared with dogs younger than 1 yr (p < 0.05). no statistical difference was observed when sex, breed, purpose, or modus vivendi was compared with seropositivity. most do ... | 2002 | 12537126 |
| first portuguese isolate of neospora caninum from an aborted fetus from a dairy herd with endemic neosporosis. | neospora caninum was isolated from the brain of an aborted 4-month-old fetus from a dairy cow herd with endemic neosporosis in porto, portugal. the fetal brain homogenate was inoculated interperitoneally first into outbred swiss webster mice given dexamethasone and then the peritoneal exudates from these mice was co-inoculated with mouse sarcoma cells in the peritoneal cavity of mice given dexamethasone. n. caninum tachyzoites were seen in peritoneal exudate of the second passage. tachyzoites fr ... | 2002 | 12446085 |
| seroprevalence of neospora caninum infection in dairy and beef cattle in paraguay. | seroprevalence of neospora caninum in 879 beef and dairy cattle in different locations of paraguay was determined by an elisa. in the survey, 262 (29.8%) cattle were positive to n. caninum, and animals with anti-neospora antibody titre were observed in all the locations tested in the country. serum samples taken from a herd that exhibited persistent abortion had the highest percentage of animals being positive to the parasite (17/30, 56.7%). in the same herd, abortion was significantly more like ... | 2002 | 12446086 |
| transplacental toxoplasmosis in a reindeer (rangifer tarandus) fetus. | toxoplasma gondii infection was diagnosed in a full term stillborn reindeer (rangifer tarandus) fetus. the fetus had encephalitis and placentitis associated with t. gondii. tissue cysts were identified histologically in sections of brain and tachyzoites were present in placenta and the myocardium. protozoa in the brain, heart, and placenta stained positively with t. gondii antibodies, but not with neospora caninum antibodies in an immunohistochemical test. the dam of the fetus had a 1:12,800 tit ... | 2002 | 12446098 |
| immune responses to neospora caninum and prospects for vaccination. | developing an effective vaccine against neosporosis presents several interesting challenges. the parasite is spread efficiently from mother to foetus over several generations, and naturally infected cattle do not appear to develop adequate protective immunity. modulation of the immune response during pregnancy favours parasite survival and multiplication. however, induction of pro-inflammatory responses that are thought to be protective against neospora caninum would be detrimental to the pregna ... | 2002 | 12473366 |
| the comparative pathogenesis of neosporosis. | since its first description in dogs in 1984, the protozoan parasite neospora caninum has been found to infect a wide range of animals, worldwide. in cattle, n. caninum has particular significance as a cause of abortion in which persistence of infection in the mother, recrudescence of the parasite during pregnancy, and the vulnerability of the placenta and foetus to invasion are important features. this article discusses how neospora invades cells, how it infects and causes disease in several ani ... | 2002 | 12482540 |
| studies on serological cross-reaction of neospora caninum with toxoplasma gondii and hammondia heydorni. | the enzyme-linked immunosorbent assay (elisa) was used to examine cross-reactivity of neospora caninum with toxoplasma gondii and hammondia heydorni. anti-t. gondii mouse and cat sera cross-reacted with n. caninum soluble antigen (nla), but not with the recombinant surface antigen (ncsrs2). anti-h. heydorni dog sera showed no cross-reactivity with either the nla antigen or the ncsrs2. lack of cross-reactivity between anti-h. heydorni sera and n. caninum antigens, and the cross-reactivity of anti ... | 2002 | 11913555 |
| quantitative detection of neospora caninum in bovine aborted fetuses and experimentally infected mice by real-time pcr. | we report the development of a real-time pcr assay for the quantitative detection of neospora caninum in infected host tissues. the assay uses the double-stranded dna-binding dye sybr green i to continuously monitor product formation. oligonucleotide primers were designed to amplify a 76-bp dna fragment corresponding to the nc5 sequence of n. caninum. a similar method was developed to quantify the 28s rrna host gene in order to compare the parasite load of different samples and to correct for th ... | 2002 | 11923330 |
| redescription of neospora caninum and its differentiation from related coccidia. | neospora caninum is a protozoan parasite of animals, which before 1984 was misidentified as toxoplasma gondii. infection by this parasite is a major cause of abortion in cattle and causes paralysis in dogs. since the original description of n. caninum in 1988, considerable progress has been made in the understanding of its life cycle, biology, genetics and diagnosis. in this article, the authors redescribe the parasite, distinguish it from related coccidia, and provide accession numbers to its t ... | 2002 | 12076623 |
| application of real-time fluorescent pcr for quantitative assessment of neospora caninum infections in organotypic slice cultures of rat central nervous system tissue. | the previously described nc5-specific pcr test for the diagnosis of neospora caninum infections was used to develop a quantitative pcr assay which allows the determination of infection intensities within different experimental and diagnostic sample groups. the quantitative pcr was performed by using a dual fluorescent hybridization probe system and the lightcycler instrument for online detection of amplified dna. this assay was successfully applied for demonstrating the parasite proliferation ki ... | 2002 | 11773124 |
| necrotizing cerebellitis due to neospora caninum infection in an old dog. | a severe, necrotizing, non-suppurative inflammation of the cerebellum associated with neospora caninum infection was identified in a 14-year-old male labrador retriever. on presentation, clinical signs included mild depression and head tremor, marked ataxia of both thoracic and pelvic limbs, and abnormal postural reactions. in the central nervous system, inflammatory lesions were mainly restricted to the cerebellar leptomeninges and cerebellar cortex, which appeared necrotic and atrophic. protoz ... | 2002 | 11913826 |
| natural transmission routes of neospora caninum between farm dogs and cattle. | twelve dairy herds with evidence of post-natal infection with neospora caninum were compared with 21 control herds with no evidence of post-natal infection. on the former farms, dogs consumed placenta or licked uterine discharge in 75 and 67% of the farms, respectively, while on control farms these activities occurred in 38 and 24% of the farms, respectively. on all control farms and all but three post-natally infected farms the dogs were fed colostrum or milk. defecation of dogs on the feeding ... | 2002 | 11900923 |
| neospora caninum and waddlia chondrophila strain 2032/99 in a septic stillborn calf. | characteristics of an intracellularly growing micro-organism isolated from an aborted bovine foetus are described. the organism replicated within cytoplasmic vacuoles, was resistant to penicillin and exhibited structural characteristics compatible with waddlia chondrophila. an elisa specific for chlamydia spp., immunofluorescence tests using antibodies directed against chlamydia spp. or simkania negevensis, and pcr using chlamydia-specific primers showed that the agent was distinct from chlamydi ... | 2002 | 11852195 |
| seroprevalence of antibodies against neospora caninum in diagnostic equine serum samples and their possible association with fetal loss. | a case-control study of the association between the presence of serum antibodies against neospora spp. and fetal loss was performed on serum samples submitted to a veterinary diagnostic laboratory in northwestern united states. control sera were randomly selected from those submitted from healthy horses for routine equine infectious anemia testing required for regulatory health certification. case sera were randomly selected from those submitted from aborting mares for diagnostic workup. based o ... | 2002 | 11934465 |
| neospora caninum is an invalid species name: an evaluation of facts and statements. | an evaluation of both the formal requirements of the international rules of zoological nomenclature and the scientific reasons for the description of new genera and species shows that the name neospora caninum is a nomen nudum. the only characteristic criteria for discriminating between the previously described species hammondia heydorni and the proposed new species n. caninum (i.e. the lack of a parasitophorous vacuole) has been shown to be wrong in many publications. furthermore, absolutely no ... | 2002 | 11936509 |
| factors associated with neospora caninum serostatus in cattle of 20 specialised costa rican dairy herds. | twenty-five specialised costa rican dairy farms (located in the poás area) were used to determine neosporosis seroprevalence and the association of seropositivity with environmental and management factors. the farms involved were selected intentionally and all of them use vampp 5.1 (veterinary automated management and production control programme) as management-information system. holstein-friesian, jersey and crosses between them were the most-frequent breeds in these herds. the number of femal ... | 2002 | 11937233 |
| infection of organotypic slice cultures from rat central nervous tissue with neospora caninum: an alternative approach to study host-parasite interactions. | neospora caninum is an apicomplexan parasite which has emerged as an important cause of bovine abortion worldwide. abortion is usually triggered by reactivation of dormant bradyzoites during pregnancy and subsequent congenital infection of the foetus, where the central nervous system appears to be most frequently affected. we here report on an organotypic tissue culture model for neospora infection which can be used to study certain aspects of the cerebral phase of neosporosis within the context ... | 2002 | 11943226 |
| companion animal parasitology: a clinical perspective. | in recent years there have been many changes to the ways that clinical veterinary science is conducted and nowhere is this more evident than in companion animal practice. veterinarians working with pet dogs and cats are facing new challenges associated with the emergence and re-emergence of parasitic diseases. some, such as neospora caninum, have been recently recognised; others like giardia and cryptosporidium have been reported with increasing frequency, in part as a result of laboratory tests ... | 2002 | 11943231 |
| use of a serum-free medium to produce in vitro neospora caninum and toxoplasma gondii tachyzoites on vero cells. | neospora caninum and toxoplasma gondii are cyst-forming coccidian parasites of human and veterinary clinical relevance. in vitro cultivation of the protozoans using vero cells is usually performed in order to produce antigenic materials. quantitative and qualitative comparisons of vero cells grown in rpmi medium supplemented either with foetal calf serum (fcs), horse serum (hs) or a specific serum-free additive (defcell) were performed. a serum-free cell culture system used to propagate n. canin ... | 2002 | 11944805 |
| prevalence of neospora caninum antibodies in dogs from dairy cattle farms in parana, brazil. | serum samples from 134 dogs from 22 cattle dairy farms in the northern region of parana state, brazil, were tested for antibodies to neospora caninum in an indirect fluorescent antibody test. antibodies (> or = 1:50) to n. caninum were found in 29 (21.6%) of the 134 dogs, and seropositive dogs were found on 14 (63.6%) of the 22 dairy cattle farrms. the antibody titers of dogs were 1:50 (3 dogs), 1:100 (7 dogs), 1:200 (7 dogs), 1:400 (6 dogs), and > or = 1:800 (6 dogs). the low prevalence (9%) in ... | 2002 | 12054023 |
| sensitive and specific identification of neospora caninum infection of cattle based on detection of serum antibodies to recombinant ncp29. | neosporosis is an economically important disease of dairy cattle caused by the protozoan neospora caninum. diagnostic tests for neosporosis are complicated by the potential for cross-reaction of antibodies to antigens that are similar between n. caninum and closely related parasites toxoplasma gondii and sarcocystis cruzi. to provide a sensitive and specific assay for detecting antibodies to n. caninum in the serum of infected animals, we have investigated a recombinant form of the antigen known ... | 2002 | 11986268 |
| immunological relationship between neospora caninum and besnoitia besnoiti. | neospora caninum is a coccidian parasite identified as a major cause of abortion in cattle. a combined infection of n. caninum with another taxonomically related parasite of cattle, besnoitia besnoiti can occur in geographical areas endemic for both species. both infections are routinely diagnosed serologically, and incorrect diagnosis could occur if immunological cross-reactivity exists between the two parasites. to investigate the possible degree of cross-reactivity, we compared results obtain ... | 2002 | 11992709 |
| identification of a neospora caninum microneme protein (ncmic1) which interacts with sulfated host cell surface glycosaminoglycans. | the invasive stages of apicomplexan parasites enter their host cells through mechanisms which are largely conserved throughout the phylum. host cell invasion is divided into two distinct events, namely, adhesion onto the host cell surface and the actual host cell entry process. the former is mediated largely through microneme proteins which are secreted at the onset of establishing contact with the host cell surface. many of the microneme proteins identified so far contain adhesive domains. we h ... | 2002 | 12011014 |
| epidemiological investigations of abortions due to neospora caninum on swiss dairy farms. | abortions apparently due to neospora caninum in two swiss dairy herds were investigated by means of a pcr and elisa, and other potential causes were eliminated. in addition, a case-control study of 24 case herds and 24 control herds indicated that n caninum-associated abortions were more likely to occur in herds with antibodies to coxiella burnetii (with an odds ratio [or] of 3.38 with a 95 per cent confidence interval [ci] of 1.82 to 6.22), whereas the likelihood was less in herds with antibodi ... | 2002 | 12019533 |
| vero cell surface proteoglycan interaction with the microneme protein ncmic(3) mediates adhesion of neospora caninum tachyzoites to host cells unlike that in toxoplasma gondii. | neospora caninum and toxoplasma gondii are characterised by a very low host cell specificity, thus they are able to infect a wide range of different cells in vivo and in vitro. infection of the host cell by tachyzoites is a process which is preceded by adhesion onto the host cell surface. the receptors on the host cell surface which would allow n. caninum to establish a physical interaction have not been investigated so far. here we report the role of host cell surface proteoglycans as receptors ... | 2002 | 12062488 |
| trigeminal neuropathy in dogs: a retrospective study of 29 cases (1991-2000). | the medical records of 29 dogs unable to close their mouths due to flaccid paralysis or paresis of the muscles innervated by the mandibular branch of the trigeminal nerve, were reviewed. idiopathic trigeminal neuropathy was diagnosed in 26 dogs based on complete resolution of clinical signs and lack of any long-term neurological disease. of these dogs, golden retrievers were overrepresented. no age, sex, or seasonal predispositions were identified. trigeminal sensory innervation deficits were ob ... | 2002 | 12022413 |
| serological survey of neospora caninum infection in dairy cattle herds in aguascalientes, mexico. | a serological survey for antibody activity to neospora caninum was carried out in aguascalientes, a state in the central part of mexico. one-hundred and eighty-seven serum samples from 13 dairy herds were tested by the elisa test. the herd prevalence was 100% and the overall prevalence was 59% (n=110). seventy-six of 97 seropositive cows had previous records of abortion. there was a statistically significant difference between the groups (p<0.05). however, the odds ratio was 1.4, suggesting an a ... | 2002 | 12031815 |
| a longitudinal study of neospora caninum infection on a dairy farm in new zealand. | a 600-cow new zealand dairy herd experienced an abortion storm in 1997 and was monitored (blood sampling at about 3-month intervals) from may 1997 until january 1999. abortion risk reached 9% in 1997 and was highest in heifers at 19%. the abortion risk decreased in 1998 to 3.2% (still somewhat higher than during the years prior to the outbreak). the serological reaction pattern for neospora caninum showed an association with abortion risk only around the time of the 1997 outbreak when seropositi ... | 2002 | 12062516 |
| immunisation of mice against neosporosis. | in the present study a murine encephalitis model was used to investigate if protection against neosporosis could be achieved by immunisation. groups of 10 mice were immunised with a sublethal dose of live neospora caninum tachyzoites, n. caninum antigens incorporated into iscoms, n. caninum lysate mixed with quil a, or n. caninum lysate in pbs. control mice were given quil a only. challenge infection with 2.5x10(6) n. caninum tachyzoites resulted in clinical symptoms that remained until the end ... | 2002 | 12062558 |
| occurrence of neospora caninum antibodies in sera from dogs of the city of são paulo, brazil. | neospora caninum is an important cause of abortion in dairy cattle worldwide. dogs are important in the epidemiology of this parasite because they are the only hosts known to excrete n. caninum oocysts. in order to understand the prevalence of n. caninum in dogs, sera from 500 owned dogs and from over 600 feral street dogs from the city of são paulo, brazil were assayed for antibodies to n. caninum. sera were examined by the neospora agglutination test (nat) using mouse-derived tachyzoites. anti ... | 2002 | 12031819 |
| hepatic sarcocystosis in a striped dolphin (stenella coeruleoalba) from the spanish mediterranean coast. | fatal hepatic sarcocystosis was diagnosed in a striped dolphin (stenella coeruleoalba) from the northeastern spanish mediterranean coast based on pathologic findings and the microscopic and ultrastructural characteristics of the intralesional parasite. main gross lesions were icterus, subcutaneous hemorrhages, and hepatic congestion. the most prominent microscopic lesions consisted of severe acute multifocal to coalescing necrotizing hepatitis with cholestasis and intralesional protozoa. there w ... | 2002 | 12053971 |
| pattern of recognition of neospora caninum tachyzoite antigens by naturally infected pregnant cattle and aborted foetuses. | different aspects of neospora tachyzoite antigen recognition by neospora-infected heifers and cows and aborted foetuses were studied. the pattern of antigen recognition and the relationship between ifat titres and number of neospora antigens detected, were evaluated. in addition, the tachyzoite antigens involved in the humoral immune response developed against infection in normal cows and cows that aborted were also characterised throughout pregnancy. comparison of tachyzoite antigen recognition ... | 2002 | 12072210 |
| susceptibility of psammomys obesus and meriones tristrami to tachyzoites of neospora caninum. | the susceptibility of psammomys obesus (sand rat) and meriones tristrami (tristram's jird) to neospora caninum was investigated by subcutaneous (s.c.) and intraperitoneal (i.p.) inoculation of 10-fold doses of culture-derived tachyzoites. groups of 5 animals were inoculated with doses of 10-10(7) parasites via each route of inoculation. all but 2 of the sand rats inoculated with doses of 10-10(4) parasites succumbed to the infection by 7-18 days postinfection. all jirds inoculated with 10(7) tac ... | 2002 | 12054004 |
| diagnosis and seroepidemiology of neospora caninum-associated bovine abortion. | a round table was conducted at the viiith international coccidiosis conference on neospora diagnosis with particular emphasis on strategies to diagnose bovine abortion. the strength and weakness of different assays for neospora caninum infection and whether these methods have resulted in the overdiagnosis of neosporosis was discussed. it was evident that each diagnostic method, namely histology, immunohistochemistry, molecular detection and serological assays were, under certain circumstances, v ... | 2002 | 11943234 |
| prevalence of antibodies to neospora caninum in white-tailed deer, odocoileus virginianus, from the southeastern united states. | serum samples from 305 white-tailed deer (odocoileus virginianus) from 14 states in the southeastern united states were examined for antibodies to neospora caninum using a direct agglutination test. positive agglutination titers were found in 145 (48%) of the white-tailed deer examined: 21 (7%) had titers of 1:25, 92 (30%) had titers of 1:50, and 32 (10%) had titers of > or = 1:500. these findings that antibodies to n. caninum are common in white-tailed deer support the concept that a sylvatic c ... | 2002 | 12054027 |
| p38-avidity-elisa: examination of herds experiencing epidemic or endemic neospora caninum-associated bovine abortion. | an enzyme-linked immunosorbent assay (elisa) was established to measure the avidity of bovine igg directed against p38, a surface antigen (ncsrs2) of neospora caninum tachyzoites. in the sera of dams intravenously infected with n. caninum nc-1, the p38-specific avidity increased from initially below 40% up to avidity indices between 50 and 80% after days 23 and 91 p.i. the p38-avidity-elisa was used to examine various herds that had experienced endemic and epidemic n. caninum-associated bovine a ... | 2002 | 12079735 |
| serological survey of neospora caninum and leishmania infantum co-infection in dogs. | a seroprevalence survey and risk analysis of neospora caninum and leishmania infantum was conducted in dogs from an area of the campania region of southern italy, in order to investigate the co-infection of these two protozoa. blood samples were collected from 1058 asymptomatic dogs over a 18 months period. serum samples were tested for antibodies to n. caninum and to l. infantum using the indirect fluorescent antibody test. epidemiological data (breed, age, sex, and utilization) were collected ... | 2002 | 12079736 |
| immune factors influencing the course of infection with neospora caninum in the murine host. | this paper investigates the role of specific immune factors on the course of infection in genetic knockout (gko) mice infected with 3 different strains of neospora caninum. survival time and parasite persistence were examined in interferon-gamma (ifn-gamma), tumor necrosis factor receptor-2 (tnfr2), interleukin 10 (il-10), beta 2 microglobulin (beta2m), and inducible nitric oxide synthase (inos2) gko or wild-type (wt) mice following infection with either pathogenic (nc-1 or nc-2) or attenuated ( ... | 2002 | 12053997 |
| dog shedding oocysts of neospora caninum: pcr diagnosis and molecular phylogenetic approach. | results of molecular determination of a dog isolate of neospora caninum in the czech republic are presented. colorless bisporocystic oocysts measuring 10-13 micro m x 10-11 micro m were recovered from feces and used for dna isolation. a diagnostic pcr procedure using previously described molecular methods was performed to determine the species. the n. caninum species-specific primers based on the nc 5 region produced a positive result, while primers specific for hammondia heydorni rdna internal ... | 2002 | 12423929 |
| neosporosis in a white rhinoceros (ceratotherium simum) calf. | a 16-day-old white rhinoceros calf died suddenly while in excellent condition and showing no obvious previous clinical signs. it was the 9th calf of a mature female kept free-ranging with 11 other rhinoceros as well as various other game species on a 2000 hectare game breeding centre adjacent to the town of lichtenburg and outlying cattle farmlands in the northern province. at post mortem examination, death was ascribed to heart failure. there was marked multifocal to coalescing subacute parasit ... | 2002 | 12088073 |
| steroid hormones do not reactivate neospora caninum in ovariectomized mice. | the direct effects of three steroid hormones (progesterone, estradiol-17beta and corticosterone) on the growth of neospora caninum (n. caninum) tachyzoite were examined in vero cells. subsequently, ovariectomized balb/c mice infected with n. caninum were treated with physiological concentrations of the steroid hormones for 1 or 2 weeks. these hormones had no direct effect on the parasite growth in vitro. in the infected mice, there was no significant difference in the parasite distribution and h ... | 2002 | 12399600 |
| coprodiagnosis of hammondia heydorni in dogs by pcr based amplification of its 1 rrna: differentiation from morphologically indistinguishable oocysts of neospora caninum. | hammondia heydorni is thought to be a non-pathogenic coccidian parasite of dogs that is closely related to neospora caninum, an important parasite of cattle and dogs. oocysts of these two species are morphologically indistinguishable from each other. a population of 2240 dogs in the czech republic was screened for the presence of h. heydorni/n. caninum oocysts and five (0.22%), represented by five of 3135 faecal samples (0.16%), were positive. the internal transcribed spacer 1 region of the rrna ... | 2002 | 12093189 |
| responses of adipose and muscle lipoprotein lipase to chronic infection and subsequent acute lipopolysaccharide challenge. | infection of male swiss webster mice with toxoplasma gondii or neospora caninum leads to long-term alterations in energy balance. following an initial 20 to 30% weight loss in all t. gondii-infected mice, half of the animals regain most of the lost weight (gainers), whereas the others maintain their low body weight (nongainers). infection with n. caninum does not elicit weight loss. lipoprotein lipase (lpl), the enzyme responsible for plasma triglyceride (tg) clearance and partitioning among tis ... | 2002 | 12093671 |