Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| the ncgra7 gene encodes the immunodominant 17 kda antigen of neospora caninum. | a neospora caninum 17-19 kda antigenic protein fraction (p17) in one-dimensional polyacrylamide gel electrophoresis (sds-page) is the immunodominant antigen recognized by sera from bovines naturally infected by n. caninum. to identify the proteins making up the p17 fraction, we screened a new n. caninum tachyzoite cdna library with an affinity-purified antibody against p17 (apa17). we isolated several cdna clones with 100% sequence identity to the ncgra7 gene. this previously described gene enco ... | 2007 | 17032479 |
| prevalence of antibodies against toxoplasma gondii and neospora caninum in hungarian red foxes (vulpes vulpes). | in the present study we have investigated the seroprevalence to the protozoan parasites toxoplasma gondii and neospora caninum in 337 red foxes (vulpes vulpes) from 16 out of 19 counties in hungary. the foxes were originally collected within a national vaccination program against rabies. antibodies to t. gondii were detected in as many as 228 (68%) of the foxes using a commercial direct agglutination test (dat). in an indirect iscom elisa, five foxes (1.5%) were positive for antibodies against n ... | 2007 | 17045742 |
| survey of neospora caninum and bovine herpes virus 1 coinfection in cattle. | a seroprevalence survey of neospora caninum and bovine herpesvirus 1 (bhv-1) was conducted in cattle pasturing in an area of the southern italian apennines to investigate the coinfection of these two pathogens. blood samples were collected from 948 pastured cattle raised on 81 farms. sera were tested for antibodies to n. caninum and to bhv-1 using an elisa assay and a neutralization test, respectively. out of the 81 farms sampled, 63 (77.8%) were positive for n. caninum and 80 (98.8%) for bhv-1. ... | 2007 | 17053931 |
| immunoanalysis of three litters born to a doberman bitch infected with neospora caninum. | neospora caninum is a tissue cyst-forming coccidium that may cause neuromuscular disorders in dogs. infected bitches can transmit the parasite to their pups in utero. vertical transmission may occur after primary infection during pregnancy and in subsequent pregnancies. the reason why only a few pups develop clinical neosporosis is unknown. we obtained sera from a doberman bitch and its offspring delivered in three litters. the bitch had a titer of 1:640 in an indirect fluorescent antibody test ... | 2007 | 17096148 |
| presence of neospora caninum specific antibodies in three dairy farms in georgia and two in texas. | neospora caninum is known to cause abortion in cattle. this study demonstrated the presence of specific igg to neospora in milk and serum samples obtained from three dairy farms in georgia and two in texas. samples from four hundred fourteen dairy cows were examined using a western blot assay of which 362 were milk and 87 were serum. samples with antibodies to neospora were identified in 32.1% (105/327) of the examined animals in georgia, whereas in texas it was identified in 10.3% (9/87). posit ... | 2007 | 17112670 |
| serologic study of anti-neospora caninum antibodies in household dogs and dogs living in dairy and beef cattle farms in tehran, iran. | a few studies have been done on the seroepidemiology of anti-neospora caninum antibodies in dairy and beef cattle farms in iran, which suggested the presence of n. caninum in these areas, but there is no published information directed on the presence or epidemiology of this organism in the dogs in iran. to investigate anti-n. caninum antibodies in household dogs and dogs living in cattle farms, 100 blood samples were collected: 50 from dogs living in dairy and beef cattle farms and 50 from house ... | 2007 | 17120042 |
| intrauterine neospora caninum inoculation of heifers and cows using contaminated semen with different numbers of tachyzoites. | to investigate the potential of different neospora caninum tachyzoite doses to infect heifers (experiment 1) and cows (experiment 2) when administered in utero by artificial insemination via contaminated semen. | 2007 | 17126895 |
| pcr-rflp for the detection and differentiation of the canine piroplasm species and its use with filter paper-based technologies. | canine piroplasmosis is an emerging disease worldwide, with multiple species of piroplasm now recognised to infect dogs. a nested polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) assay was developed for the detection and differentiation of each of the piroplasm species currently known to infect dogs on the basis of the 18s ribosomal rna gene. the assay can potentially amplify and discriminate between theileria annae, theileria equi, babesia conradae, babesia gibsoni, ... | 2007 | 17127005 |
| multimammate rat (mastomys natalensis), tristram's jird (meriones tristrami) and wagner's gerbil (gerbillus dasyurus) as laboratory models of acute neosporosis. | to test the different sensitivity of rodents of the subfamily murinae and gerbillinae, wagner's gerbils (gerbillus dasyurus), tristram's jirds (meriones tristrami) and multimammate rats (mastomys natalensis) were inoculated with neospora caninum tachyzoites. clinical signs of neosporosis appeared in all inoculated animals. histopathological examination confirmed the presence of tachyzoites in brains, lungs, skeletal muscle, myocardium, liver, in serosa of stomach and intestines, and in vesicular ... | 2007 | 17134728 |
| structure-activity relationships from in vitro efficacies of the thiazolide series against the intracellular apicomplexan protozoan neospora caninum. | nitazoxanide (2-acetolyloxy-n-(5-nitro 2-thiazolyl) benzamide; ntz) represents the parent compound of a novel class of broad-spectrum anti-parasitic compounds named thiazolides. ntz is active against a wide variety of intestinal and tissue-dwelling helminths, protozoa, enteric bacteria and a number of viruses infecting animals and humans. while potent, this poses a problem in practice, since this obvious non-selectivity can lead to undesired side effects in both humans and animals. in this study ... | 2007 | 17141783 |
| immunization of cattle with live tachyzoites of neospora caninum confers protection against fetal death. | neospora caninum is an obligate intracellular protozoan parasite that causes abortion in cattle. it is normally found as a latent infection controlled by a t-helper-cell type 1 response involving cd4(+) cytotoxic t cells and gamma interferon. cattle may be infected by two different routes: transplacentally as a result of activation of the latent infection in the mother causing congenital infection or abortion and by ingestion of oocysts, which, if it occurs during gestation, can also result in a ... | 2007 | 17145943 |
| apical membrane antigen 1 is a cross-reactive antigen between neospora caninum and toxoplasma gondii, and the anti-ncama1 antibody inhibits host cell invasion by both parasites. | the cross-reactive antigens of neospora caninum and toxoplasma gondii are important in the exploration to determine the common mechanisms of parasite-host interaction. in this study, a gene encoding n. caninum apical membrane antigen 1 (ncama1) was identified by immunoscreening of a n. caninum tachyzoite cdna expression library with antisera from mice immunized with recombinant t. gondii apical membrane antigen 1 (tgama1). ncama1 was encoded by an open reading frame of 1695 bp, which encoded a p ... | 2007 | 17156863 |
| progesterone supplementation during mid-gestation increases the risk of abortion in neospora-infected dairy cows with high antibody titres. | progesterone has been reported to reduce th1 responses during pregnancy. thus, given that an excessive th1 response could lead to abortion in neospora-infected dairy cows, we hypothesized a reduction in abortion risk following progesterone supplementation during the mid-gestation period in these animals. seropositive animals were assigned on day 120 of gestation to a control (n=33) or treatment (n=34) group. treatment consisted of progesterone supplementation by fitting a progesterone releasing ... | 2007 | 17182187 |
| host/parasite relationship in the in vitro infection of rat gliocytes by neospora caninum: evaluation of cell respiration. | neospora caninum is a protozoon that causes abortion in cattle and neuromuscular lesions in dogs, with the formation of cysts mainly in the central nervous system. since n. caninum is an intracellular parasite with tropism for the cells of nervous system, this study evaluated the respiratory metabolism of glial cells infected by this* parasite. glial cultures obtained from the cerebral cortex of newborn rats were kept in dmem enriched with 10% fetal bovine serum, 1 mm pyruvic acid and 2 mm of l- ... | 2007 | 17184800 |
| neospora caninum emerges from the shadow of toxoplasma gondii. | it is sometimes easy to make the mistake of assuming that everything that holds true for toxoplasma gondii is also true for its relative neospora caninum. however, a recurring theme in the recent review by hemphill et al. is not the similarities but the striking differences between the two parasites. | 2007 | 17185039 |
| analysis of the immune response to neospora caninum in a model of intragastric infection in mice. | to study experimental neospora caninum infection initiated at the gastrointestinal tract, toll-like receptor 4- and functional il-12rbeta2 chain-deficient c57bl/10 sccr mice were challenged intragastrically with 5 x 10(6) n. caninum tachyzoites. all parasite-inoculated mice eventually died with disseminated infection. in contrast, immunocompetent balb/c mice challenged with 1 x 10(7) n. caninum tachyzoites by the intragastric (i.g.) or the intraperitoneal (i.p.) route remained alive for at least ... | 2007 | 17187652 |
| natural killer cells act as early responders in an experimental infection with neospora caninum in calves. | the intracellular protozoan parasite neospora caninum is a cause of abortion and congenital disease in cattle worldwide. we have previously shown that natural killer (nk) cells produce ifn-gamma in response to n. caninum tachyzoites in vitro. this study aimed to investigate the role of nk cells and other cellular immune responses in an experimental n. caninum infection model in calves. phenotyping of peripheral blood lymphocytes showed a drop in the percentage of nk cells at days 4-6 after i.v. ... | 2007 | 17188277 |
| neospora caninum antibodies detected in midwestern white-tailed deer (odocoileus virginianus) by western blot and elisa. | white-tailed deer (odocoileus virginianus) serve to maintain the neospora caninum life cycle in the wild. sera from white-tailed deer from south central wisconsin and southeastern missouri, usa were tested for antibodies to n. caninum by western blot analyses and two indirect elisas. seroreactivity against n. caninum surface antigens was observed in 30 of 147 (20%) of wi deer and 11 of 23 (48%) of mo deer using western blot analysis. compared to western blot, the two indirect elisas were found t ... | 2007 | 17194547 |
| aetiology of bovine abortion in switzerland from 1986 to 1995--a retrospective study with emphasis on detection of neospora caninum and toxoplasma gondii by pcr. | in a retrospective study, covering the period from 1986 to 1995, tissues of aborted fetuses were re-examined. a total of 347 cases were tested immunohistochemically, among them samples of 223 brains were examined for neospora caninum, toxoplasma gondii and bovine virus diarrhoea virus (bvdv), and 249 placentae for chlamydiaceae. two real-time pcr assays, one for n. caninum, and one for t. gondii, were developed. these potential abortion-inducing agents were detected - and confirmed by pcr, excep ... | 2007 | 17359449 |
| comparative host-parasite relationships in ovine toxoplasmosis and bovine neosporosis and strategies for vaccination. | toxoplasma gondii and neospora caninum are important causes of reproductive loss in ruminant species worldwide. both parasites cause disease during pregnancy that may result in foetal death or birth of live congenitally infected offspring. t. gondii is also an important human pathogen with the main risk groups including pregnant women and immuno-compromised individuals, although clinical disease has also been observed in outbreaks among immuno-competent people. while the two parasites are closel ... | 2007 | 17367899 |
| study on the prevalence of toxoplasma gondii and neospora caninum and molecular evidence of encephalitozoon cuniculi and encephalitozoon (septata) intestinalis infections in red foxes (vulpes vulpes) in rural ireland. | thoracic fluid (pleural fluid and clotted blood) from 206 foxes were examined for antibodies to toxoplasma gondii and 220 thoracic fluid samples were tested for neospora caninum antibodies using indirect immunofluorescent antibody tests (ifat). a total of 115 (56%) and six (3%) foxes had antibodies to t. gondii and n. caninum, respectively. the brains from 148 foxes were examined for histological lesions and pathological changes suggestive of parasitic encephalitis were observed in 33 (22%). two ... | 2007 | 17368946 |
| peripheral immune responses in pregnant cattle following neospora caninum infection. | experimental infection of cattle with neospora caninum in early gestation causes foetal death, but the foetus survives infection in late gestation. an immunological mechanism of abortion has been suggested; therefore changes in the maternal immune response during pregnancy could account for these differences. we have investigated the peripheral immune responses of pregnant cattle following an intravenous inoculation with 10(7) n. caninum tachyzoites in early and late gestation. percentages of cd ... | 2007 | 17371459 |
| molecular characterisation of bsr4, a novel bradyzoite-specific gene from neospora caninum. | here we present the identification and cloning of the ncbsr4 gene, the putative neospora caninum orthologue to the toxoplasma gondii tgbsr4 gene. to isolate ncbsr4, genome walking pcr was performed on n. caninum genomic dna using the expressed sequence tag ncest3c28h02.y1 sequence, which shares a 44% identity with the tgbsr4 gene, as a framework. nucleotide sequencing of amplified dna fragments revealed a single uninterrupted 1227 bp open reading frame that encodes a protein of 408 amino acids w ... | 2007 | 17376452 |
| prevalence of neospora caninum and persistent infection with bovine viral diarrhea virus in dairy-breed steers in a feedlot. | to determine the prevalence and effect of neospora caninum infection and persistent infection (pi) with bovine viral diarrhea virus (bvdv) on weight gain, morbidity, and mortality rate in dairy-breed steer calves located on a feedlot in california. | 2007 | 17397345 |
| toxoplasma gondii dysregulates ifn-gamma-inducible gene expression in human fibroblasts: insights from a genome-wide transcriptional profiling. | toxoplasma gondii is an obligate intracellular parasite that persists for the life of a mammalian host. the parasite's ability to block the potent ifn-gamma response may be one of the key mechanisms that allow toxoplasma to persist. using a genome-wide microarray analysis, we show here a complete dysregulation of ifn-gamma-inducible gene expression in human fibroblasts infected with toxoplasma. notably, 46 of the 127 ifn-gamma-responsive genes were induced and 19 were suppressed in infected cell ... | 2007 | 17404298 |
| identification of ribosomal phosphoprotein p0 of neospora caninum as a potential common vaccine candidate for the control of both neosporosis and toxoplasmosis. | the characterization of the cross-reactive antigens of two closely related apicomplexan parasites, neospora caninum and toxoplasma gondii, is important to elucidate the common mechanisms of parasite-host interactions. in this context, a gene encoding n. caninum ribosomal phosphoprotein p0 (ncp0) was identified by immunoscreening of a n. caninum tachyzoite cdna expression library with antisera from mice immunized with t. gondii tachyzoites. the ncp0 was encoded by a gene with open reading frame o ... | 2007 | 17412435 |
| a longitudinal study of seroprevalence and seroconversion of neospora caninum infection in dairy cattle in northeast thailand. | a long-term study was carried out in 11 dairy herds in the khon kaen province of northeast thailand between august 2001 and november 2004. the objective was to investigate seroprevalence dynamics of neospora caninum infection in the herds and to demonstrate patterns of seroconversion in individual cattle. each herd was visited once a year, in total four times, and sera from cattle > 3 months of age and farm dogs as well as a sample from the bulk milk were collected. all samples were analysed for ... | 2007 | 17416467 |
| epidemiology and control of neosporosis and neospora caninum. | neospora caninum is a protozoan parasite of animals. until 1988, it was misidentified as toxoplasma gondii. since its first recognition in dogs in 1984 and the description of the new genus and species neospora caninum in 1988, neosporosis has emerged as a serious disease of cattle and dogs worldwide. abortions and neonatal mortality are a major problem in livestock operations, and neosporosis is a major cause of abortion in cattle. although antibodies to n. caninum have been reported, the parasi ... | 2007 | 17428888 |
| isolation and molecular detection of neospora caninum from naturally infected sheep from brazil. | neospora caninum was isolated from a naturally infected sheep from brazil by bioassay in dogs. approximately 70g of brain from each of two 4-month-old sheep with indirect fluorescent antibodies (>or=1:50) to n. caninum was offered to a different ifat negative dog (sheep n. 302, ifat 1:400-dog 1 and sheep n. 342, ifat 1:50-dog 2). parasite dna was detected in both sheep brains using a pcr targeting the nc-5 gene of n. caninum. shedding of neospora-like oocysts was noticed only in dog 1, from 10 d ... | 2007 | 17451882 |
| seroprevalence of antibodies to neospora caninum in urban and rural dogs in north-west italy. | sera were collected from 490 dogs from north-west italy. one hundred and eighty-eight dogs were urban, while 302 dogs were rural. among the latter, 190 were shepherd dogs and 112 were cattle farm dogs. sera were tested for the presence of antibodies against neospora caninum using the neospora agglutination test. seroprevalence at 1/40, 1/80, 1/160 dilution titres was significantly higher in rural (36.4%, 19.5%, 9.9% respectively) than in urban dogs (20.2%, 10.6%, 4.8% respectively). seroprevalen ... | 2007 | 17456144 |
| detection of pathogens in ovine and caprine abortion samples from sardinia, italy, by pcr. | during 2003-2005, 399 abortion samples (315 fetuses and 84 placentae) were collected from 107 ovine and caprine farms in northern sardinia. tissues from aborted fetuses and placentae were examined by pcr assay to detect dna from coxiella burnetii, chlamydophila abortus, salmonella enterica serovar abortusovis, toxoplasma gondii, and neospora caninum. the dna from at least 1 of these 5 infectious agents was amplified in 41% of ovine fetuses, while only 17% of the caprine fetuses yielded a positiv ... | 2007 | 17459840 |
| necrotizing encephalitis of unknown cause in fennoscandian arctic foxes (alopex lagopus). | a neurologic disease affected a colony of endangered fennoscandian arctic foxes (alopex lagopus) kept in captivity for breeding purposes. several outbreaks of disease occurred between 1994 and 2004. the clinical signs included ataxia, indications of anosmia, blindness, and abnormal behavior. the disease was characterized by severe necrotizing encephalitis affecting mostly the cranial cerebrum, basal ganglia, and olfactory bulbs. investigations to identify the etiology of the disease included tes ... | 2007 | 17459845 |
| chronic neospora caninum infection and repeat abortion in dairy cows: a 3-year study. | the serological status of neospora was monitored in animals older than 6 months in a dairy herd with a 3-year history of prevalent n. caninum and n. caninum-associated abortions. the numbers of animals in the herd tested each year of the study period were 259, 222 and 231, respectively. a separate analysis was performed on the 122 animals persisting in the herd for the 3 years. the overall seroprevalence of n. caninum in the herd decreased from 31.7% in the first year to 24.8% in the second year ... | 2007 | 17467905 |
| serosurvey of infectious disease agents of carnivores in captive red pandas (ailurus fulgens) in china. | the future of the endangered red panda (ailurusfulgens) depends in part on the development of protective measures against infectious diseases. the present study is a first step toward improved understanding of infectious diseases in the species' home regions. serum samples obtained from 73 red pandas in 10 captive facilities in southwest, east, and northeast china from october to december 2004 were tested for antibodies against nine common infectious pathogens of carnivores. antibody titers agai ... | 2007 | 17469274 |
| induction of tachyzoite egress from cells infected with the protozoan neospora caninum by nitro- and bromo-thiazolides, a class of broad-spectrum anti-parasitic drugs. | neospora caninum represents an important pathogen causing stillbirth and abortion in cattle and neuromuscular disease in dogs. nitazoxanide (ntz) and its deacetylated metabolite tizoxanide (tiz) are nitro-thiazolyl-salicylamide drugs with a broad-spectrum anti-parasitic activity in vitro and in vivo. in order to generate compounds potentially applicable in food and breeding animals, the nitro group was removed, and the thiazole-moiety was modified by other functional groups. we had shown earlier ... | 2007 | 17481636 |
| seroepidemiology of toxoplasma gondii and neospora caninum in seals around hokkaido, japan. | serological analysis was performed to detect toxoplasma gondii and neospora caninum infection in seals in hokkaido. serum samples were collected from 322 kuril harbor seals (phoca vitulina stejnegeri) at nosappu, akkeshi and erimo, from 46 spotted seals (p. largha) at nosappu, erimo, yagishiri island, hamamasu and syakotan, and from 4 ribbon seals (p. fasciata) and a bearded seal (erignathus barbatus) at nosappu between 1998 and 2006. recombinant surface antigen of t. gondii (sag2t) and n. canin ... | 2007 | 17485927 |
| seroprevalence of and agroecological risk factors for mycobacterium avium subspecies paratuberculosis and neospora caninum infection among adult beef cattle in cow-calf herds in alberta, canada. | a province-wide cross-sectional seroprevalence and agroecological risk factor study of mycobacterium avium subspecies paratuberculosis (map) and neospora caninum (nc) infection among cattle in 100 cow-calf herds in alberta was conducted. the seroprevalence of map in adult cattle was 1.5% across all herds. using a widely accepted herd test cutpoint of 2 or more seropositive cows out of 30 animals tested, 7.9% of herds were estimated to be infected (95% confidence interval (ci): 2.3-23.4%). seropr ... | 2007 | 17494367 |
| detection of toxoplasma gondii in water by an immunomagnetic separation method targeting the sporocysts. | an immunomagnetic separation (ims) method was developed to detect toxoplasma gondii in fresh waters by using the monoclonal antibody 4b6 targeting the sporocyst wall of t. gondii, hammondia hammondi, hammondia heydorni, and neospora caninum. water concentrates obtained by filtering 10- to 20-l samples samples were spiked with toxoplasma oocysts, sonicated to release the sporocysts, and analyzed by ims-4b6. mean sporocyst recoveries were 74.5 +/- 5.3% in drinking water, 30.6 +/- 2.4 and 37.1 +/- ... | 2007 | 17530288 |
| use of an enzyme-linked immunosorbent assay in bulk milk to estimate the prevalence of neospora caninum on dairy farms in prince edward island, canada. | this study evaluated the use of bulk milk as a diagnostic tool for estimation of herd-level neospora caninum exposure in atlantic canada; it was used to estimate the prevalence of dairy farms with a within-herd n. caninum-seroprevalence > or = 15% in prince edward island (pei). the variation over time of n. caninum antibodies in bulk milk is also reported. skimmed bulk milk and individual serum samples were analyzed for n. caninum antibodies by using an enzyme-linked immunosorbent assay (elisa). ... | 2007 | 17542367 |
| treatment of canine pediatric neospora caninum myositis following immunohistochemical identification of tachyzoites in muscle biopsies. | a 7-week-old irish wolfhound was evaluated for an abnormal hind limb gait. quadriceps muscle atrophy was pronounced and patellar reflexes were absent bilaterally. neospora caninum myositis was diagnosed by histopathologic and serologic examination and immunohistochemical staining of muscle. substantial clinical improvement was noted after 18 weeks of treatment with clindamycin. | 2007 | 17542369 |
| neosporosis and hammondiosis in dogs. | the dog is a definitive host of the protozoan parasite neospora caninum, and in many parts of the world, infection is relatively common as determined by serology. reported seroprevalences usually range from 0 to 20 per cent, however, reports of clinically affected dogs are infrequent. affected dogs are generally less than six months old and predominantly have signs of an ascending hindleg paralysis, with the associated lesions of polyradiculoneuritis and granulomatous polymyositis. although any ... | 2007 | 17547641 |
| factors associated with variation in neospora caninum bulk-milk s/p ratios in initially bulk-milk negative testing dutch dairy herds. | we conducted a study on 81 initially bulk-milk elisa negative dairy herds taken from a random sample of dutch dairy herds to evaluate variation in bulk-milk s/p ratios and to study reasons for bulk-milk conversion. these herds were repeatedly (3-month intervals) tested between april 2004 and august 2005 and serostatus of all animals had previously been established as negative (n), low-positive (lp) or high-positive (hp). of these herds, herd- and test-related factors associated with variation in ... | 2007 | 17566576 |
| prevention of lethal experimental infection of c57bl/6 mice by vaccination with brucella abortus strain rb51 expressing neospora caninum antigens. | bovine abortions caused by the intracellular protozoal parasite neospora caninum are a major concern to cattle industries worldwide. a strong th1 immune response is required for protection against n. caninum. brucella abortus strain rb51 is currently used as a live, attenuated vaccine against bovine brucellosis. strain rb51 can also be used as an expression vector for heterologous protein expression. in this study, putative protective antigens of n. caninum mic1, mic3, gra2, gra6 and srs2, were ... | 2007 | 17568587 |
| prevention of vertical transmission of neospora caninum in c57bl/6 mice vaccinated with brucella abortus strain rb51 expressing n. caninum protective antigens. | bovine abortions caused by the apicomplexan parasite neospora caninum have been responsible for severe economic losses to the cattle industry. infected cows either experience abortion or transmit the parasite transplacentally at a rate of up to 95%. neospora caninum vaccines that can prevent vertical transmission and ensure disruption in the life cycle of the parasite greatly aid in the management of neosporosis in the cattle industry. brucella abortus strain rb51, a commercially available vacci ... | 2007 | 17575983 |
| the apicomplexan pathogen neospora caninum inhibits host cell apoptosis in the absence of discernible nf-kappa b activation. | neospora caninum, a causative agent of bovine abortions, is an apicomplexan parasite that is closely related to the human pathogen toxoplasma gondii. since a number of intracellular parasites, including t. gondii, have been shown to modulate host cell apoptosis, the present study was conducted to establish whether n. caninum is similarly capable of subverting apoptotic pathways in its host cells. our results indicated that death receptor-mediated apoptosis is repressed during n. caninum infectio ... | 2007 | 17576757 |
| possible role of bovine trophoblast giant cells in transplacental transmission of neospora caninum in cattle. | neospora caninum is an aplicomplexan parasite that has brought several concerns to cattle raisers worldwide due to its relationship to fetal loss. however, the mechanism of the parasite's transplacental infection and induced abortions are not completely understood. bovine trophoblastic binucleated cells (bnc) play a major role in the maternal-fetal interactions, migrating during the entire pregnancy from chorionic connections to uterine epithelium. this study aimed to investigate the possible ro ... | 2007 | 17588318 |
| development of two immunochromatographic tests for the serodiagnosis of bovine babesiosis. | in this study, we developed two immunochromatographic tests (icts), which are nitrocellulose membrane-based immunoassays for the convenient and rapid serodiagnosis of bovine babesiosis caused by babesia bovis (boict) and babesia bigemina (biict). the efficacy of two icts was evaluated using 13 positive sera from experimentally infected cattle with b. bovis or b. bigemina. clear results showed that the boict and elisa detected antibodies in sera collected from 14 to 93 days post-infection, while ... | 2007 | 17590275 |
| neospora caninum in cattle: experimental infection with oocysts can result in exogenous transplacental infection, but not endogenous transplacental infection in the subsequent pregnancy. | whilst it is presumed that infection of pregnant cattle with neospora caninum oocysts can provoke abortion and is the likely cause of epidemic abortion outbreaks, only two previous experiments have involved inoculation of pregnant cows with oocysts (and only one abortion was provoked in 22 pregnancies). here, we describe the oral oocyst challenge of 18 cows synchronously bred and inoculated precisely at 70 (n=6), 120 (n=6) and 210 (n=6) days in pregnancy with a nominal dose of 40,000 oocysts. on ... | 2007 | 17624353 |
| quantification of vertical and horizontal transmission of neospora caninum infection in dutch dairy herds. | ninety-six of 108 randomly selected dutch dairy herds had one or more cows with a positive serostatus for n. caninum. in these 96 herds, we have quantified the probabilities of vertical transmission (vt) and horizontal transmission (ht) of n. caninum infection by combining serostatus and pedigree data in 4091 dam-daughter pairs. the probability of animals infected by vertical transmission during pregnancy (prob(vt)) was calculated as the proportion of seropositive daughters among daughters of se ... | 2007 | 17640807 |
| dynamics of anti-neospora caninum antibodies during gestation in chronically infected dairy cows. | the dynamics of antibody production against neospora caninum during the gestation period was examined in chronically infected dairy cows. data were obtained from 86 pregnant parous dairy cows, 21 of which had suffered abortion. the cows belonged to two herds in which a diagnosis of n. caninum infection had been previously confirmed in aborted foetuses. pregnancy diagnosis and blood collection were performed on post-insemination days 40, 90, 120, 150, 180, 210, and at parturition or until the tim ... | 2007 | 17643822 |
| reduced cerebral infection of neospora caninum in balb/c mice vaccinated with recombinant brucella abortus rb51 strains expressing n. caninum srs2 and gra7 proteins. | neospora caninum, an obligate intracellular protozoan parasite, is the causative agent of bovine neosporosis, an important disease affecting the reproductive performance of cattle worldwide. currently there is no effective vaccine available to prevent n. caninum infection in cattle. in this study, we examined the feasibility of developing a live, recombinant n. caninum vaccine using brucella abortus vaccine strain rb51 as the expression and delivery vector. we generated two recombinant rb51 stra ... | 2007 | 17651896 |
| evidence of neospora caninum dna in wild rodents. | seventy-five house mice (mus musculus), 103 rats (rattus norvegicus) and 55 field mice (apodemus sylvaticus) from north-west italy were pcr analysed for neospora caninum infection. brain, kidney and muscle tissues collected from the above mentioned animals were tested by pcr using np6 and np21 primers. the brain tissue from 2 house mice and 2 rats, the kidney from 4 rats, 1 house mouse and 1 field mouse and muscle from 10 rats, 8 house mice and 1 field mouse were tested positive for n. caninum. ... | 2007 | 17651897 |
| neospora caninum infection in birds: experimental infections in chicken and embryonated eggs. | neospora caninum causes economical impact in cattle-raising farms since it is implicated as the major cause of bovine abortions. although infection by the parasite has been widely described in mammals, the role of birds in its life-cycle is still obscure. therefore, this work aimed to evaluate the infection by n. caninum in different chicken models. experimental infections were conducted in 7-day-old chicks, laying hens and embryonated eggs, where samples were analysed for parasite burden, igg a ... | 2007 | 17686190 |
| seroprevalence of neospora caninum infection in dogs from rural and urban environments in tehran, iran. | 103 serum samples were taken from owned dogs in tehran (53 urban dogs and 50 farm dogs) from february to may 2005 and tested for igg antibody against neospora caninum using the immunofluorescence antibody test. urban dogs were selected from apparent healthy dogs referred to the small animal hospital of faculty of veterinary medicine, university of tehran, for routine operation, and the farm dogs were selected from the sheepdogs in sheep flocks around tehran randomly. the titer of 1:50 was select ... | 2007 | 17687566 |
| possibility of neospora caninum infection by venereal transmission in cb-17 scid mice. | cb-17 scid and balb/c male mice were inoculated intraperitoneally with neospora caninum to examine the possibility of its venereal transmission. some of these mice were killed on days 7 and 20 post-inoculation to examine the genital organs for presence of the parasite. the remaining scid male mice were housed with non-infected female mice from day 7 p.i. and kept with them for 14 days. these scid mice died between days 28 and 35 p.i. n. caninum dna was detected in the testis of mice on days 7 an ... | 2007 | 17689194 |
| humoral immune response in pregnant heifers inoculated with neospora caninum tachyzoites by conjunctival route. | the aim of this study was to compare systemic humoral immune responses in pregnant heifers inoculated with neospora caninum tachyzoites by conjunctival and intravenous routes. twenty nine heifers separated in three experimental groups were studied: group 1 (n=10 animals) and group 2 (n=9 animals) were inoculated with 10(8) of n. caninum tachyzoites by conjunctival and intravenous routes at 5th month of gestation, respectively; group 3 (n=10 animals) were non-inoculated control animals. an indire ... | 2007 | 17697754 |
| cloning and expression of an antigenic domain of a major surface protein (nc-p43) of neospora caninum. | neospora caninum is an obligate intracellular protozoan that can infect domestic and wild canids, as well as ruminants and equines. it was described in 1988 as causing neuromuscular alterations and death in dogs. recently, n. caninum has been the focus of considerable attention for its large impact on the dairy industry, given the economic losses related to breeding failures and to a decrease in productivity. elisa diagnosis of neosporosis has not been widely used in brazil, mostly because of th ... | 2007 | 17706005 |
| prevalence of anti-toxoplasma gondii and anti-neospora caninum antibodies in goats slaughtered in the public slaughterhouse of patos city, paraíba state, northeast region of brazil. | the prevalence of anti-toxoplasma gondii and anti-neospora caninum antibodies was investigated in goats slaughtered in the public slaughterhouse of patos, state of paraíba, northeast region of brazil, and possible associations between sex of the animals and antibody prevalence were verified. three-hundred six blood samples from goats collected before slaughter by jugular venopuncture were used. for the serologic diagnosis of t. gondii and n. caninum, the indirect fluorescent-antibody test (ifat) ... | 2007 | 17706359 |
| first identification of neospora caninum infection in aborted bovine foetuses in china. | the first identification of neospora caninum infection in the tissues of aborted bovine foetuses in china is reported. aborted foetuses were collected from 16 dams, and 12 of the dams had high serum antibody titres to n. caninum determined using an elisa test kit. the nc-5 gene of n. caninum was amplified from dna samples extracted from brains of four aborted foetuses using a neospora-specific pcr assay, confirming n. caninum infection in the aborted foetuses. histology and immunohistochemistry ... | 2007 | 17706874 |
| sarcocystis sp.-associated meningoencephalitis in a bald eagle (haliaeetus leucocephalus). | protozoal meningoencephalitis is uncommon in raptors. an adult female bald eagle (haliaeetus leucocephalus) was euthanized after several months of treatment for progressive neurologic signs. the predominant histologic lesion was lymphoplasmacytic and histiocytic meningoencephalitis involving the cerebrum and cerebellum. there was a marked segmental loss of granular cells and purkinje cells, as well as segmental atrophy of the molecular layer in the cerebellum. protozoal merozoites and schizonts ... | 2007 | 17823405 |
| cutaneous toxoplasmosis in a female japanese cat. | a 16-year-old female japanese cat was presented with a single mammary-gland nodule approximately 3 cm in diameter. histologically, the nodule consisted of necrotizing granulomatous panniculitis, vasculitis, and mastitis, and contained free and clustered protozoal organisms. the organism was present in the cytoplasm of macrophages, fibroblasts, endothelial cells, and mammary-gland epithelia. the organism was positive for anti- toxoplasma gondii and anti- neospora caninum antibodies. electron micr ... | 2007 | 17846241 |
| internal hydrocephalus and associated periventricular encephalitis in a young fox. | marked lateral ventricular enlargement associated with atrophic cerebral cortex and periventricular encephalitis is described in a 2-month-old fox affected by disorientation, generalized ataxia, difficulty in walking, circling, and blindness. clinical conditions progressed to stupor and spontaneous death within a few days. at necropsy, severe inflammatory and necrotizing lesions were observed in periventricular sites associated with diverticula and cleft formation in perithalamic areas and rhine ... | 2007 | 17846249 |
| isolation of neospora caninum from dairy zero grazing cattle in israel. | first israeli neospora caninum isolates were obtained from brain tissues of aborted fetuses (ncis491 and ncis580) from dairy farms endemic for neosporosis and maintaining cattle on zero grazing. tissues from different parts of the fetus brains were used to infect vero cells. tachyzoites of n. caninum were first observed in cultures from days 30 and 32 after infection. to confirm the identity of the isolated parasites, dna extracts from brains and cultures were tested by pcr with specific primers ... | 2007 | 17850972 |
| protection against abortion linked to gamma interferon production in pregnant dairy cows naturally infected with neospora caninum. | many immunological aspects of pregnancy, such as the role played by gamma interferon (ifn-gamma) in abortion, are not well understood. neospora caninum is an intracellular protozoan considered to be among the main causes of abortion in cattle worldwide. the present study analyzes the interaction between ifn-gamma production and n. caninum infection in naturally infected pregnant cows. data were obtained from 126 pregnant cows: 86 seropositive and 40 seronegative for the parasite. pregnancy diagn ... | 2007 | 17854883 |
| fecal and serological survey of neospora caninum in farm dogs in costa rica. | to detect oocysts of neospora caninum in dog feces and to determine the excretion pattern in dogs from specialized dairy farms in costa rica, a total of 265 fecal samples from 34 dogs were collected at intervals from february to august 2005. fecal samples were examined for n. caninum-like oocysts microscopically, by dna detection using the polymerase chain reaction (pcr), and by bioassay. n. caninum dna was detected by pcr in four fecal samples, twice from one dog, but oocysts were not detected ... | 2007 | 17868998 |
| neosporosis in beagle dogs: clinical signs, diagnosis, treatment, isolation and genetic characterization of neospora caninum. | clinical neosporosis was diagnosed in a litter of five pups born to a beagle bitch from virginia, usa. four of the pups developed limb weakness starting at 4 weeks of age. the dogs were suspected to have neosporosis based on clinical signs and empirically treated with clindamycin (75 mg, oral, twice daily, total 150 mg) starting at 9 weeks of age and the dosage was doubled at 13 weeks of age. antibodies to neospora caninum were detected in sera of the dam and pups when first tested serologically ... | 2007 | 17890012 |
| bayesian kriging of seroprevalence to mycobacterium avium subspecies paratuberculosis and neospora caninum in alberta beef and dairy cattle. | identifying spatial patterns of risk is important in the study of diseases with ecologic causes. furthermore, relatively complex hierarchical modeling is required to determine how factors that are organized across levels interact, such as how an ecologic cause interacts with farm management and with animal characteristics. the objective of this study was to map the risk for mycobacterium avium subspecies paratuberculosis (map - the causative agent of johne's disease) and neospora caninum (nc - t ... | 2007 | 18189052 |
| generation of parasite cysts in cultured cells instead of living animals. | in order to develop any effective means of prevention or therapy against neospora caninum infection, all three life cycle stages of this parasite have to be taken into account. in this project, the in vitro culture of n. caninum bradyzoites containing tissue cysts has been developed, thus allowing to the initiation of stage conversion without the use of animal experimentation. this enables researchers to study novel aspects of stage differentiation, to investigate novel diagnostic antigens, and ... | 2007 | 19835051 |
| plasma pregnancy-associated glycoprotein-1 (pag-1) concentrations during gestation in neospora-infected dairy cows. | the aim of this study was to determine whether plasma pregnancy-associated glycoprotein-1 (pag-1) concentrations in pregnancy are affected by persistent neospora caninum infection in dairy cows. the data analyzed were derived from 22 multiparous cows: 16 n. caninum-seropositive and 6 n. caninum-seronegative animals (used as controls). three of the 16 seropositive cows aborted during the study period and the corresponding data were analyzed separately. pregnancy diagnoses were performed on day 40 ... | 2007 | 17010415 |
| seroepidemiology of neospora caninum and toxoplasma gondii in cattle and water buffaloes (bubalus bubalis) in the people's republic of china. | a seroepidemiological survey of neospora caninum and toxoplasma gondii in cattle and water buffaloes was carried out in the people's republic of china. serum samples were obtained from dairy (n=262, 9 herds in 9 provinces) and beef cattle (n=10, 1 herd) and water buffaloes (n=40) in china. all sera were tested for antibodies to n. caninum and t. gondii by an enzyme-linked immunosorbent assay (elisa) and an indirect agglutination test (iat), respectively. the overall seroprevalence of n. caninum ... | 2007 | 17010521 |
| first report of neospora caninum-associated bovine abortion in mashhad area, iran. | neospora caninum is an apicomplexan protozoan which causes abortion in cattle worldwide. the present study was designed to assess the importance of bovine neosporosis for causing abortion in iranian cattle. infection was primarily diagnosed by polymerase chain reaction (pcr), complemented with histopathology and immunohistochemistry (ihc). one hundred brains of aborted bovine fetuses were collected from iranian dairy herd between 2003 and 2005 in mashhad area. n. caninum was detected by pcr in t ... | 2007 | 17024355 |
| the first detection of neospora caninum dna in the colostrum of infected cows. | limited data is available on the vertical transmission of neospora caninum via the colostrum. the results of our previous research revealed the presence of n. caninum dna in the milk of seropositive cows. the aim of the present work is to demonstrate parasite dna in colostrum samples. a polymerase chain reaction using np21 and np6 primers was applied to dna isolated from the colostrum sediment in order to amplify the corresponding genomic nc-5 region. the expected 328-bp product was obtained in ... | 2007 | 17024360 |
| neospora caninum detected in feral rodents. | the role of rodents in the epidemiology of neosporosis was investigated by assaying brain tissue of feral mice (mus musculus) and rats (rattus norvegicus) for neospora caninum. both mouse and rat brain tissue were extracted for total dna, and subjected to two different n. caninum-specific nested polymerase chain reaction (pcr) assays. a portion of brain tissue from the mice and rats were also assayed for n. caninum in gerbils or gamma-interferon gene knockout (ko) mice. of the 105 feral mice tes ... | 2007 | 16997474 |
| evaluation of serological tests for the diagnosis of neospora caninum infection in dogs: optimization of cut off titers and inhibition studies of cross-reactivity with toxoplasma gondii. | diagnosis of neospora caninum infection in dogs is based on serological assays such as the indirect fluorescent antibody test (ifat) and enzyme-linked immunosorbent assays (elisa). this study evaluated two serological tests (ifat and elisa) for the detection of igg antibodies to n. caninum in 300 serum samples of dogs through the optimization of cut off titers by using the two-graph receiver-operating characteristic (tg-roc) curve. in addition, the identification of major cross-reactive antigens ... | 2007 | 16973287 |
| serodiagnosis of neospora caninum infection in cattle using a recombinant tncsrs2 protein-based elisa. | the truncated ncsrs2 gene (tncsrs2) by removal of the n-terminal hydrophobic sequence was cloned into the pgex-6p-1 plasmid and subsequently expressed as a glutathione-s-transferase (gst) fusion protein. the purified recombinant tncsrs2 protein was specific to neospora caninum and was used in an elisa for the diagnosis of neosporosis. there was a good agreement between tnctsrs2-based elisa and three commercially available diagnostic kits (idexx elisa, hipra elisa and vmrd ifat). three hundred da ... | 2007 | 16989950 |
| comparison of serological methods for the diagnosis of neospora caninum infection in cattle. | the aims of this study were to evaluate the performance and agreement of various commercial and in-house neospora caninum antibody assays used in dairy cattle in north america, and to investigate reproducibility of two assays performed in different laboratories. from 1998 to 2005, three enzyme linked immunosorbent assays (elisas, a competitive elisa-vmrd inc., an indirect elisa-biovet inc., and another indirect elisa-herdchek idexx corp.), two indirect fluorescent antibody tests (ifats, vmrd inc ... | 2007 | 16989951 |
| seroprevalence of neospora caninum in non-carnivorous wildlife from spain. | serum samples from 1034 non-carnivorous wildlife from spain were tested for antibodies to neospora caninum by competitive screening enzyme linked immunosorbent assay (elisa) and confirmed by an indirect fluorescent antibody test (ifat). high agreement was observed between results in both techniques (kappa value higher than 0.9). prevalences of n. caninum antibodies positive by both techniques were 11.8% of 237 red deer (cervus elaphus), 7.7% of 13 barbary sheep (ammotragus lervia), 6.1% of 33 ro ... | 2007 | 16962706 |
| serological survey of neospora caninum in dogs in the czech republic and a long-term study of dynamics of antibodies. | the main aim of the present study was to establish the prevalence of antibodies against neospora caninum dogs from the czech republic and to examine the dynamics of antibody titers during a long-term period. for this purpose, sera of 858 dogs were examined for the presence of anti-n. caninum antibodies using an indirect immunofluorescent antibody test (ifat). four groups of dogs of various origins were included in the survey: the first group (a, n=470) comprised dogs purchased by the czech army ... | 2007 | 16956728 |
| antibodies to neospora caninum and toxoplasma gondii in domestic cats from brazil. | the occurrence of antibodies to neospora caninum and toxoplasma gondii was determined in 400 domestic cats (siamese, persian, and undetermined breeds) from the municipality of araçatuba, sao paulo, brazil, through the indirect fluorescence antibody test (ifat). of the 400 cats, 100 were seropositive to t. gondii (25%, titer > or =64) and 98 to n. caninum (24.5%, titer > or =16). the rate of seropositive cats for t. gondii was correlated with age (chi(2)=35.7; p<0.001), with a higher number of in ... | 2007 | 16941188 |
| neospora caninum: high susceptibility to the parasite in c57bl/10sccr mice. | c57bl/10sccr mice, lack toll-like receptor 4 and a functional interleukin-12 receptor. taking this into account, susceptibility of these mice to neospora caninum infection was assessed comparatively to that of immunocompetent c57bl/10scsn mice. c57bl/10sccr mice inoculated intraperitoneally with 5x10(5)n. caninum tachyzoites showed a high susceptibility to this parasite. all infected c57bl/10sccr mice were dead by day 8 post-infection whereas all control c57bl/10scsn mice survived this parasitic ... | 2007 | 16889774 |
| [determination and correlation of anti-neospora caninum antibodies in cattle and dogs from paraná, brazil]. | the protozoan parasite neospora caninum is a major cause of abortion in cattle worldwide. the aim of the present study was to determine and to compare the presence of anti-neospora caninum antibodies in cattle, as well as in farm dogs, and the relationship among them. the research was conducted in different mesoregions of paraná state, southern brazil. sera from 1.263 cattle, from 77 farms were tested for antibodies to n. caninum by elisa. sera from 129 dogs, from 35 farms were tested for antibo ... | 2008 | 20059847 |
| interleukin-12 response to ncsrs2 immunization of balb/c mice against neospora caninum. | the apicomplexan parasite neospora caninum can cause abortions and is recognized as an important causative agent responsible for economic and reproductive losses in the cattle industry. humoral immune response was investigated in balb/c mice by using recombinant ncsrs2 expressed in escherichia coli as polyhistidine-tagged fusion proteins. ncsrs2 is the major immunodominant tachyzoite surface antigen of n. caninum. separate groups of female balb/c mice were infected subcutaneously with (p) pbs, ( ... | 2008 | 20059851 |
| [seroprevalence of antibodies anti-neospora caninum and anti-toxoplasma gondii in captive wild canids]. | neosporosis is considered one of the main cause of abortion in dairy cattle in the world. the prevalence of neospora caninum in wild species has been studied since the coyote (canis latrans), a north american wild canid specie was discovered as definitive host of this parasite. the aim of the present study was to determine the serum prevalence of n. caninum and t. gondii in wild native canids species from brazil. serum samples of 25 crab-eating dogs (cerdocyon thous), five pampas fox (lycalopex ... | 2008 | 20059860 |
| [risk factors and prevalence of antibodies anti-neospora caninum in urban and rural dogs from rio grande do sul, brazil]. | serum samples from 339 dogs from rural (beef and dairy farms) and urban areas of municipality of pelotas, rs, were tested by indirect fluorescent antibody test to verify the presence of antibodies anti-neospora caninum. data from dogs took part of an epidemiologic questionnaire to identify possible risk factors associated with the seropositivity. antibodies anti-n. caninum were found 15.6% (53/339) of serum samples, with 5.5% (6/109) of positive dogs from urban area and 20.4% (47/230) from rural ... | 2008 | 20059865 |
| [epidemiologic survey of neospora caninum in bovines of rural settlements in corumbá, ms]. | neospora caninum, is an obligate intracellular parasite, can infect domestic and wild dogs, equines and ruminants. it was described in 1988, and its economic importance is attributed to costs of abortion, the value of the fetuses, artificial insemination and coverage, the decrease in milk production, the increase in the disposal and replacement of animals. in four rural settlements of the municipality of corumba, ms (mato grande, paiolzinho, taquaral and tamarineiro) was used a combination of qu ... | 2008 | 20059867 |
| occurrence of toxoplasma gondii and hammondia hammondi oocysts in the faeces of cats from germany and other european countries. | faecal samples of 24,106 cats from germany and other european countries were examined microscopically in a veterinary laboratory in germany between october 2004 and november 2006 to estimate the prevalence of animals shedding toxoplasma gondii or hammondia hammondi oocysts. oocysts of 9-15 microm size with a morphology similar to that of h. hammondi and t. gondii were found in 74 samples (0.31%). a total of 54 samples were further characterised to achieve a species diagnosis and to determine the ... | 2008 | 18226453 |
| studies on neospora caninum dna detection in the oocytes and embryos collected from infected cows. | neosporosis is a major cause of abortion in cattle over the world. one of the methods of preventing vertical transmission within the herd is to avoid breeding replacement heifers from infected dams. another procedure suggested and recommended by the international embryo transfer society (iets) is embryo transfer (et) from infected dams into uninfected recipients. oocytes and embryos taken from seropositive cows were examined for the presence of neospora caninum dna. a modified pcr protocol using ... | 2008 | 18977601 |
| isolation and genetic characterization of neospora caninum from asymptomatic calves in spain. | neospora caninum is a cyst-forming parasite that causes abortion in cattle. despite this parasite's ubiquitous distribution and wide host range, the number of n. caninum isolates obtained to date is limited. in vitro isolation of the parasite is arduous and often unsuccessful. in addition, most isolates have been obtained from clinically affected hosts and therefore could be biased towards more virulent isolates. in this report, an improved isolation approach from transplacentally infected newbo ... | 2008 | 18980700 |
| neospora caninum: detection in wild rabbits and investigation of co-infection with toxoplasma gondii by pcr analysis. | neospora caninum is an important pathogen of cattle causing significant economic loss. there is much current interest in wild animal reservoirs for this parasite. the role of the rabbit in this is currently unknown. dna samples from the brains of wild rabbits (oryctolagus cuniculus) collected from the malham area of the yorkshire dales were investigated by species-specific pcr for the presence of n. caninum and toxoplasma gondii. we found prevalences of n. caninum of 10.5% (6/57) and t. gondii o ... | 2008 | 18703054 |
| seroepidemiological study of neospora caninum infection in dogs found in dairy farms and urban areas of aguascalientes, mexico. | the objective of this study was to describe the seroepidemiology of neospora caninum infection in dogs in close contact with dairy cattle and dogs from urban areas of aguascalientes, mexico, as well as to estimate the possible association between age, gender and size of these groups and seroprevalence. sera were obtained from samples of 152 dogs in dairy farms and 116 in the urban area, and were subjected to elisa test. general seroprevalence reached 32%, while the seroprevalence in farm dogs (4 ... | 2008 | 18722716 |
| occurrence of antibodies to neospora caninum and toxoplasma gondii in dogs from pernambuco, northeast brazil. | a serological survey was carried out to assess the occurrence of anti-neospora caninum antibodies in dogs from the state of pernambuco. a total of 625 serum samples of dogs (289 from paulista, 168 from amaraji and 168 from garanhuns) were tested by an immunofluorescence antibody assay for the detection of anti-n. caninum antibodies. a total of 177 (28.3%; ic 95%, 24.9-32.1) samples were positive. the seropositivity rates found in paulista, amaraji and garanhuns were 26% (ic 95%, 21-31.4), 26.2% ... | 2008 | 18723288 |
| serological survey of neospora caninum infection among dogs in japan through species-specific elisa. | a seroepidemiological survey of neospora caninum infection among dogs in japan was conducted using species-specific enzyme-linked immunosorbent assay with recombinant surface antigen (nc-sag1t). among 1,206 dogs examined, 126 dogs (10.4%) from 30 prefectures from hokkaido to okinawa were positive to n. caninum infections, which were more frequently detected in females than males. siberian huskies showed the highest positive rate compared with the other breeds. dogs with pyometra and diabetes mel ... | 2008 | 18772569 |
| evaluation of recombinant proteins of neospora caninum as vaccine candidates (in a mouse model). | abortion, resulting from infections by the parasite neospora caninum, is a major cause of economic loss to both the dairy and beef industries of cattle-producing countries of the world. vaccination as a means of preventing abortion and/or infection represents a viable control strategy; indeed a commercial vaccine is available in some countries, albeit of unknown efficacy. the commercial vaccine is based on inactivated tachyzoites of n. caninum but other approaches based on lysates and recombinan ... | 2008 | 18789996 |
| a four year longitudinal sero-epidemiology study of neospora caninum in adult cattle from 114 cattle herds in south west england: associations with age, herd and dam-offspring pairs. | neosporosis caused by the protozoan parasite neospora caninum, is an economically important cause of abortion, stillbirth, low milk yield, reduced weight gain and premature culling in cattle. consequently, a seroepidemiological study of n. caninum antibodies was conducted in england with 29,782 samples of blood taken from 15,736 cattle from 114 herds visited on three occasions at yearly intervals. herds were categorised into lower (< 10%) and higher (> or = 10%) median herd seroprevalence. hiera ... | 2008 | 18793402 |
| usefulness of rncgra7- and rncsag4-based elisa tests for distinguishing primo-infection, recrudescence, and chronic bovine neosporosis. | bovine reproductive failure caused by the parasite neospora caninum is a major problem and is responsible for severe economic losses worldwide. currently, appropriate control measures depend on the predominant transmission route in a particular herd. therefore, the development of diagnostic tools capable of discriminating between primo-infection, recrudescence, re-infection, and chronic infection is a major challenge in the serodiagnosis of bovine neosporosis. here, two recombinant protein-based ... | 2008 | 18814972 |
| spatial analysis of neospora caninum distribution in dairy cattle from sweden. | the national herd prevalence and spatial distribution of neospora caninum infected dairy herds in sweden were investigated. the study was based on a bulk milk survey comprising samples from 2,978 herds. test-positive herds were found in all parts of sweden and the overall prevalence of test-positive herds was 8.3% (95% confidence interval = 7.3-9.3%). the presence of spatial autocorrelation was tested using the moran's i test. possible clusters of test-positive herds were identified by applying ... | 2008 | 19021107 |
| current trends in follow-up of trophoblastic function in ruminant species. | during the pregnancy of ruminants, different hormones and proteins are secreted by placenta or corpus luteum allowing the follow up of gestation. among them, progesterone (p4) and pregnancy-associated glycoproteins (pag) were proposed as laboratory tools to establish or to confirm pregnancy diagnosis. in last years, pag assay also provided useful information for researchers working in programs focused on the follow up of trophoblastic function. concentrations of pag appeared as altered after the ... | 2008 | 19261972 |
| [frequency of anti-neospora caninum antibodies in cows and fetuses from municipalities of southern minas gerais]. | the objective of this study was to determine the frequency of antibodies anti-neospora caninum in cows and fetuses from municipalities of southern minas gerais. a total of 559 serum samples of cows of the a bank of sera formed from of serum samples collected at 18 dairy farms, distributed for seven municipalities of the lavras micro region, southern minas gerais, and 575 serum samples of cows and 503 serum samples of fetuses were collected at slaughter house of campo belo, mg. serum samples were ... | 2008 | 19265576 |
| frequency of antibodies against neospora caninum in stray and domiciled dogs from urban, periurban and rural areas from paraná state, southern brazil. | antibodies to neospora caninum were analyzed by an indirect fluorescent antibody test (ifat), in stray and domiciled dogs from urban, periurban and rural areas from curitiba, paraná state, brazil. of the total of 556 dogs, 18.17% (101) were positive for n. caninum (titer 50). frequency of antibody was 12.71 in urban, 15.73 in periurban, and 25.38% in rural dogs. rural dogs had a significantly higher frequency of antibodies than urban dogs (p<0.05). there were no significant differences between t ... | 2008 | 19265582 |
| review of idiopathic eosinophilic meningitis in dogs and cats, with a detailed description of two recent cases in dogs. | eosinophilic meningoencephalitis (eme) has been described in various species of animals and in humans. in dogs it has been associated with protozoal infections, cuterebral myiasis and various other aetiologies. ten cases of idiopathic eosinophilic meningoencephalitis have been reported in dogs and one in a cat where the origin was uncertain or unknown. the dogs were all males, of various breeds but with a predominance of golden retrievers and rottweilers; they generally had a young age of onset. ... | 2008 | 19496320 |