Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| various bioactivity and relationship of structure-activity of matrine analogues. | for the first time, the botanic source natural product matrine was reported to have more potent inhibitory activity against tobacco mosaic virus (tmv) than the commercial virucide ribavirin. on the basis of the structural diversity modification strategy, a series of matrine derivatives was synthesized and systematically evaluated for their antiviral activity against tmv, fungicidal activity, and insecticidal activity. as a result, compounds 3 (inhibitory rate 67.3%, 69.5%, 63.7%, 63.0% at 500 μg ... | 2017 | 28248103 |
| comparisons of transcriptional profiles of gut genes between cry1ab-resistant and susceptible strains of ostrinia nubilalis revealed genes possibly related to the adaptation of resistant larvae to transgenic cry1ab corn. | a microarray developed on the basis of 2895 unique transcripts from larval gut was used to compare gut gene expression profiles between a laboratory-selected cry1ab-resistant (r) strain and its isoline susceptible (s) strain of the european corn borer (ostrinia nubilalis) after the larvae were fed the leaves of transgenic corn (mon810) expressing cry1ab or its non-transgenic isoline for 6 h. we revealed 398 gut genes differentially expressed (i.e., either up- or down-regulated genes with express ... | 2017 | 28146087 |
| insecticidal spectrum and mode of action of the bacillus thuringiensis vip3ca insecticidal protein. | the vip3ca protein, discovered in a screening of spanish collections of bacillus thuringiensis, was known to be toxic to chrysodeixis chalcites, mamestra brassicae and trichoplusia ni. in the present study, its activity has been tested with additional insect species and we found that cydia pomonella is moderately susceptible to this protein. vip3ca (of approximately 90kda) was processed to an approximately 70kda protein when incubated with midgut juice in all tested species. the kinetics of prot ... | 2017 | 27756652 |
| high regional genetic diversity and lack of host-specificity in ostrinia nubilalis (lepidoptera: crambidae) as revealed by mtdna variation. | the european corn borer (ostrinia nubilalis) infests a wide array of host plants and is considered one of the most serious pests of maize in europe. recent studies suggest that individuals feeding on maize in europe should be referred to o. nubilalis (sensu nov.), while those infesting dicots as ostrinia scapulalis (sensu nov.). we test if the clear genetic distinctiveness among individuals of o. nubilalis living on maize vs. dicots is tracked by mitochondrial dna (mtdna). we used fragments of c ... | 2016 | 27019346 |
| a fish-based chromosome map for the european corn borer yields insights into ancient chromosomal fusions in the silkworm. | a significant feature of the genomes of lepidoptera, butterflies and moths, is the high conservation of chromosome organization. recent remarkable progress in genome sequencing of lepidoptera has revealed that syntenic gene order is extensively conserved across phylogenetically distant species. the ancestral karyotype of lepidoptera is thought to be n=31; however, that of the most well-studied moth, bombyx mori, is n=28, and diverse studies suggest that three chromosomal fusion events occurred i ... | 2016 | 26264548 |
| cry1f resistance among lepidopteran pests: a model for improved resistance management? | the cry1fa protein from the bacterium bacillus thuringiensis (bt) is known for its potential to control lepidopteran pests, especially through transgenic expression in maize and cotton. the maize event tc1507 expressing the cry1fa toxin gene became commercially available in the united states in 2003 for the management of key lepidopteran pests including the european corn borer, ostrinia nubilalis, and the fall armyworm, spodoptera frugiperda. a high-dose/refuge strategy has been widely adopted t ... | 2016 | 27436741 |
| additive effects on the improvement of insecticidal activity: design, synthesis, and insecticidal activity of novel pymetrozine derivatives. | a series of new pymetrozine analogues containing both methyl on the imine carbon and phenoxy group at the pyridine ring were designed and synthesized. their insecticidal activities against bean aphid (aphis craccivora), mosquito larvae (culex pipiens pallens), cotton bollworm (helicoverpa armigera), corn borer (ostrinia nubilalis) and oriental armyworm (mythimna separata) were evaluated. the results of bioassays indicated that most of the target compounds showed good insecticidal activity agains ... | 2016 | 26342545 |
| effects of feeding transgenic corn with mcry1ac or maroacc gene to laying hens for 12 weeks on growth, egg quality and organ health. | the objective of the present study was to investigate the effect of feeding two transgenic corn lines containing the mcry1ac gene from bacillus thuringiensis strain (bt-799) and the maroacc gene from agrobacterium tumefaciens strain (cc-2), respectively, on growth, egg quality and organ health indicators. expression of the mcry1ac gene confers resistance to pyrausta nubilalis and the maroacc gene confers tolerance to herbicides. healthy hens (n=96 placed in cages; 3 hens/cage) were randomly assi ... | 2016 | 26915544 |
| novel hydrazone derivatives containing pyridine amide moiety: design, synthesis, and insecticidal activity. | a series of novel hydrazone derivatives containing pyridine amide moiety were designed, synthesized, and evaluated for their insecticidal activity. bioassays indicated that some of the target compounds exhibited good insecticidal activities against nilaparvata lugens (n. lugens), plutella xylostella (p. xylostella), mythimna separata (m. separata), helicoverpa armigera (h. armigera), pyrausta nubilalis (p. nubilalis), and culex pipiens pallens (c. pipiens pallens). in particular, compound 5j rev ... | 2016 | 26832221 |
| synthesis and antiviral, insecticidal, and fungicidal activities of gossypol derivatives containing alkylimine, oxime or hydrazine moiety. | gossypol is a part of the cotton plant's defense system against pathogens and herbivorous insects. to discover gossypol analogs with broad spectrum and high activity, a series of gossypol alkylamine schiff base, oxime and hydrazone derivatives were synthesised and bioassayed. the biological results indicated that most of these derivatives exhibited higher anti-tmv activity than gossypol. interestingly, the activities of compounds 10, 15, 18, 20, 23 and 26 were much higher than that of ribavirin. ... | 2016 | 26337022 |
| a high-throughput, in-vitro assay for bacillus thuringiensis insecticidal proteins. | a high-throughput, in-vitro assay for bacillus thuringiensis (bt) insecticidal proteins designated as cry was developed and evaluated for screening a large number of cry protein variants produced by dna shuffling. this automation-amenable assay exploits an insect cell line expressing a single receptor of bt cry proteins. the cry toxin used to develop this assay is a variant of the cry1ab protein called ip1-88, which was produced previously by dna shuffling. cell mortality caused by the activated ... | 2016 | 26524384 |
| semiautomated identification of european corn borer (lepidoptera: crambidae). | the european corn borer ostrinia nubilalis (hübner, 1796) is a serious and widely studied pest of corn. the most common method of its control is by means of insecticides. however, biological control is becoming more and more popular. the hymenopteran parasitoid trichogramma sp. is the most promising and effective one among the biological agents and is now widely used in north america and europe. its application should occur at the time when the european corn borer is at the beginning of the eggs ... | 2016 | 26487742 |
| bacillus thuringiensis toxin resistance mechanisms among lepidoptera: progress on genomic approaches to uncover causal mutations in the european corn borer, ostrinia nubilalis. | transgenic plants that express bacillus thuringiensis (bt) crystal (cry) protein toxins (bt crops) effectively control feeding by the european corn borer, ostrinia nubilalis, although documented resistance evolution among a number of species in both the laboratory and field has heightened concerns about the durability of this technology. research has provided major insights into the mutations that alter bt toxin binding receptor structure and function within the midgut of lepidoptera that direct ... | 2016 | 27436734 |
| transcriptional analysis of susceptible and resistant european corn borer strains and their response to cry1f protoxin. | despite a number of recent reports of insect resistance to transgenic crops expressing insecticidal toxins from bacillus thuringiensis (bt), little is known about the mechanism of resistance to these toxins. the purpose of this study is to identify genes associated with the mechanism of cry1f toxin resistance in european corn borer (ostrinia nubilalis hübner). for this, we compared the global transcriptomic response of laboratory selected resistant and susceptible o. nubilalis strain to cry1f to ... | 2015 | 26220297 |
| aquatic degradation of cry1ab protein and decomposition dynamics of transgenic corn leaves under controlled conditions. | the increasing cultivation of genetically modified corn plants (zea mays) during the last decades is suggested as a potential risk to the environment. one of these genetically modified variety expressed the insecticidal cry1ab protein originating from bacillus thuringiensis (bt), resulting in resistance against ostrinia nubilalis, the european corn borer. transgenic litter material is extensively studied regarding the decomposition in soils. however, only a few field studies analyzed the fate of ... | 2015 | 25553417 |
| horizontal transfer of a non-autonomous helitron among insect and viral genomes. | the movement of mobile elements among species by horizontal transposon transfer (htt) influences the evolution of genomes through the modification of structure and function. helitrons are a relatively new lineage of dna-based (class ii) transposable elements (tes) that propagate by rolling-circle replication, and are capable of acquiring host dna. the rapid spread of helitrons among animal lineages by htt is facilitated by shuttling in viral particles or by unknown mechanisms mediated by close o ... | 2015 | 25766741 |
| linkage of an abcc transporter to a single qtl that controls ostrinia nubilalis larval resistance to the bacillus thuringiensis cry1fa toxin. | field evolved resistance of insect populations to bacillus thuringiensis (bt) crystalline (cry) toxins expressed by crop plants has resulted in reduced control of insect feeding damage to field crops, and threatens the sustainability of bt transgenic technologies. a single quantitative trait locus (qtl) that determines resistance in ostrinia nubilalis larvae capable of surviving on reproductive stage transgenic corn that express the bt cry1fa toxin was previously mapped to linkage group 12 (lg12 ... | 2015 | 26093031 |
| identification of a new cry1i-type gene as a candidate for gene pyramiding in corn to control ostrinia species larvae. | pyramiding of diverse cry toxin genes from bacillus thuringiensis with different modes of action is a desirable strategy to delay the evolution of resistance in the european corn borer (ostrinia nubilalis). considering the dependency of susceptibility to cry toxins on toxin binding to receptors in the midgut of target pests, a diverse mode of action is commonly defined as recognition of unique binding sites in the target insect. in this study, we present a novel cry1ie toxin gene (cry1ie2) as a ... | 2015 | 25795679 |
| role of the european corn borer (ostrinia nubilalis) on contamination of maize with 13 fusarium mycotoxins. | the european corn borer (ecb) plays an important role in promoting fusarium verticillioides infections and in the consequent fumonisin contamination in maize grain in temperate areas. the objective of this study was to evaluate whether the ecb feeding activity could also affect the occurrence of emerging mycotoxins in maize kernels. during the 2008-10 period, natural infestation of the insect was compared, in field research, with the protection of infestation, which was obtained by using an ento ... | 2015 | 25266165 |
| redefinition of nosema pyrausta (perezia pyraustae paillot 1927) basing upon ultrastructural and molecular phylogenetic studies. | populations of european corn borer (ostrinia nubilalis hübner) from krasnodar territory (southwestern russia) become regularly infected with nosema-like microsporidia. to identify the parasite, it was subjected to electron microscopy and small subunit ribosomal rna (ssu rrna) gene sequencing. the spore ultrastructure of the parasite was highly similar to nosema bombycis from china and nosema pyrausta from the usa. the nucleotide sequence of ssu rrna gene was identical to a microsporidium isolate ... | 2015 | 25563606 |
| development and characterization of microsatellite markers for rice leaffolder, cnaphalocrocis medinalis (guenée) and cross-species amplification in other pyralididae. | rice leaffolder, cnaphalocrocis medinalis (guenée), is a destructive and widespread pest on rice. in this study, 20 microsatellite markers were isolated and characterized from c. medinalis partial genomic libraries using the method of fast isolation by aflp of sequence containing repeats. of these markers, 18 markers displayed polymorphisms. polymorphisms were evaluated in 48 individuals from two natural populations. the number of alleles per locus ranged from 2 to 15, and the expected and obser ... | 2014 | 24381106 |
| effect of maize lines on larval fitness costs of cry1f resistance in the european corn borer (lepidoptera: crambidae). | crops producing insecticidal toxins from the bacterium bacillus thuringiensis (bt) are widely planted and enable management of key insect pests while reducing the use of conventional insecticides. however, the evolution of bt resistance could diminish these benefits. fitness costs of bt resistance occur in the absence of bt toxin when individuals with resistance alleles show a reduction in fitness relative to susceptible individuals, and they can delay the evolution of resistance. ecological fac ... | 2014 | 24772559 |
| the effects of bt corn on rusty crayfish (orconectes rusticus) growth and survival. | bt crops are one of the most commonly used genetically modified crops worldwide. bt crops contain a gene that is derived from the bacteria bacillus thuringiensis, which produces the cry1ab toxin. bt corn that contains the cry1ab toxin is used throughout the midwest united states to control crop pests such as the european corn borer (ostrinia nubilalis). headwater streams in regions known for intensive agriculture receive bt corn detritus after the fall harvest, which is then consumed by a divers ... | 2014 | 25001246 |
| different binding sites for bacillus thuringiensis cry1ba and cry9ca proteins in the european corn borer, ostrinia nubilalis (hübner). | binding studies using (125)i-cry9ca and biotinylated-cry1ba proteins showed the occurrence of independent binding sites for these proteins in ostrinia nubilalis. our results, along with previously available binding data, indicate that combinations of cry1a or cry1fa proteins with cry1ba and/or cry9ca could be a good strategy for the resistance management of o. nubilalis. | 2014 | 24799046 |
| susceptibility and aversion of spodoptera frugiperda (lepidoptera: noctuidae) to cry1f bt maize and considerations for insect resistance management. | bacillus thuringiensis (bt) maize was developed primarily for north american pests such as european corn borer (ostrinia nubilalis (hübner)). however, most bt maize products are also cultivated outside of north america, where the primary pests may be different and may have lower susceptibility to bt toxins. fall armyworm (spodoptera frugiperda je smith) is an important pest and primary target of bt maize in central and south america. s. frugiperda susceptibility to cry1f (expressed in event tc15 ... | 2014 | 24665722 |
| changes in gene expression in the larval gut of ostrinia nubilalis in response to bacillus thuringiensis cry1ab protoxin ingestion. | we developed a microarray based on 2895 unique transcripts assembled from 15,000 cdna sequences from the european corn borer (ostrinia nubilalis) larval gut. this microarray was used to monitor gene expression in early third-instar larvae of bacillus thuringiensis (bt)-susceptible o. nubilalis after 6 h feeding on diet, with or without the bt cry1ab protoxin. we identified 174 transcripts, for which the expression was changed more than two-fold in the gut of the larvae fed cry1ab protoxin (p < 0 ... | 2014 | 24704690 |
| determination of areas with the most significant shift in persistence of pests in europe under climate change. | this study aimed to estimate the impact of climate change on the ranges of crop pest species in europe. the organisms included in the study were species from the family tortricidae (cydia pomonella, lobesia botrana) and the family pyralidae (ostrinia nubilalis), chrysomelidae beetles (leptinotarsa decemlineata, oulema melanopus) and species from the family aphididae (ropalosiphum padi, sitobion avenae). climate conditions in the year 2055 were simulated using a subset of five representative glob ... | 2014 | 23901033 |
| mosquitocidal carbamates with low toxicity to agricultural pests: an advantageous property for insecticide resistance management. | insecticide resistance in the malaria mosquito anopheles gambiae is well documented, and widespread agricultural use of pyrethroids may exacerbate development of resistance when pyrethroids are used in vector control. we have developed carbamate anticholinesterases that possess a high degree of an. gambiae:human selectivity for enzyme inhibition. the purpose of this study was to assess the spectrum of activity of these carbamates against other mosquitoes and agricultural pests. | 2014 | 25185896 |
| draft genome sequences of two bacillus thuringiensis strains and characterization of a putative 41.9-kda insecticidal toxin. | in this work, we report the genome sequencing of two bacillus thuringiensis strains using illumina next-generation sequencing technology (ngs). strain hu4-2, toxic to many lepidopteran pest species and to some mosquitoes, encoded genes for two insecticidal crystal (cry) proteins, cry1ia and cry9ea, and a vegetative insecticidal protein (vip) gene, vip3ca2. strain leapi01 contained genes coding for seven cry proteins (cry1aa, cry1ca, cry1da, cry2ab, cry9ea and two cry1ia gene variants) and a vip3 ... | 2014 | 24784323 |
| using rna sequencing to characterize female reproductive genes between z and e strains of european corn borer moth (ostrinia nubilalis). | reproductive proteins often evolve rapidly and are thought to be subject to strong sexual selection, and thus may play a key role in reproductive isolation and species divergence. however, our knowledge of reproductive proteins has been largely limited to males and model organisms with sequenced genomes. with advances in sequencing technology, lepidoptera are emerging models for studies of sexual selection and speciation. by profiling the transcriptomes of the bursa copulatrix and bursal gland f ... | 2014 | 24621199 |
| a screening of five bacillus thuringiensis vip3a proteins for their activity against lepidopteran pests. | five bacillus thuringiensis vip3a proteins (vip3aa, vip3ab, vip3ad, vip3ae and vip3af) and their corresponding trypsin-activated toxins were tested for their toxicity against eight lepidopteran pests: agrotis ipsilon, helicoverpa armigera, mamestra brassicae, spodoptera exigua, spodoptera frugiperda, spodoptera littoralis, ostrinia nubilalis and lobesia botrana. toxicity was first tested at a high dose at 7 and 10 days. no major differences were found when comparing protoxins vs. trypsin-activat ... | 2014 | 24508583 |
| efficacy of pyramided bt proteins cry1f, cry1a.105, and cry2ab2 expressed in smartstax corn hybrids against lepidopteran insect pests in the northern united states. | commercial field corn (zea mays l.) hybrids transformed to express some or all of the lepidopteran insect-resistant traits present in smartstax corn hybrids were evaluated for insecticidal efficacy against a wide range oflepidopteran corn pests common to the northern united states, during 2008 to 2011 at locations in 15 states. smartstax hybrids contain a pyramid of two bacillus thuringiensis (bt) derived events for lepidopteran control: event tc1507 expressing cry1f protein and mon 89034 expres ... | 2014 | 24665726 |
| understanding successful resistance management: the european corn borer and bt corn in the united states. | the european corn borer, ostrinia nubilalis hübner (lepidoptera: crambidae) has been a major pest of corn and other crops in north america since its accidental introduction nearly a hundred years ago. wide adoption of transgenic corn hybrids that express toxins from bacillus thuringiensis, referred to as bt corn, has suppressed corn borer populations and reduced the pest status of this insect in parts of the corn belt. continued suppression of this pest, however, will depend on managing potentia ... | 2014 | 22688691 |
| insecticidal activity of bacillus thuringiensis cry1bh1 against ostrinia nubilalis (hubner) (lepidoptera: crambidae) and other lepidopteran pests. | bacillus thuringiensis is an important source of insect resistance traits in commercial crops. in an effort to prolong b. thuringiensis trait durability, insect resistance management programs often include combinations of insecticidal proteins that are not cross resistant or have demonstrable differences in their site of action as a means to mitigate the development of resistant insect populations. in this report, we describe the activity spectrum of a novel b. thuringiensis cry protein, cry1bh1 ... | 2013 | 24077715 |
| identification and characterization of two sensory neuron membrane proteins from cnaphalocrocis medinalis (lepidoptera: pyralidae). | sensory neuron membrane proteins (snmps), which are located on the dendritic membrane of olfactory neurons, were considered as important components involved in pheromone reception in insects. in drosophila melanogaster, mutants without snmp are unable to evoke neuronal activities in the presence of pheromone cis-vaccenyl acetate (cva). so deeply understanding the snmps functions may help to develop pheromone-mediated insect pest management tactics. the present study reports the identification an ... | 2013 | 23027616 |
| comparative binding of cry1ab and cry1f bacillus thuringiensis toxins to brush border membrane proteins from ostrinia nubilalis, ostrinia furnacalis and diatraea saccharalis (lepidoptera: crambidae) midgut tissue. | the european (ostrinia nubilalis hübner) and asian corn borers (ostrinia furnacalis guenée) are closely related and display similar sensitivity to cry1 toxins. in this study, we compared the binding patterns of cry1ab and cry1f toxins between both ostrinia spp., as well as the expression of putative cadherin- and aminopeptidase-n (apn)-like protein receptors. additionally, cdna sequences of these putative toxin receptors from both ostrinia species were compared. ligand blots for both species ind ... | 2013 | 23999243 |
| implications of european corn borer, ostrinia nubilalis, infestation in an aspergillus flavus-biocontrolled corn agroecosystem. | a novel biocontrol strategy consisting of field application of bioplastic-based granules inoculated with a non-toxigenic aspergillus flavus l. strain has recently been shown to be effective for reducing aflatoxin contamination in corn. this study focused on other factors that may affect the feasibility of this biocontrol technique, and more specifically the role of the european corn borer (ecb), ostrinia nubilalis h., in the dispersal and infestation of a. flavus in corn and its impact on crop y ... | 2013 | 23436551 |
| de novo transcriptomic resources for two sibling species of moths: ostrinia nubilalis and o. scapulalis. | this study aimed at enhancing the transcriptomic resources for two sibling species of moths, ostrinia scapulalis (adzuki bean borer) and ostrinia nubilalis (european corn borer), as a foundation for future researches on their divergence history. previous works on these species had shown that their genetic divergence was low, while they were reproductively isolated in natura and specialized on different host plants. comparative genomic resources will help facilitate the understanding of the mecha ... | 2013 | 23445568 |
| unlinked genetic loci control the reduced transcription of aminopeptidase n 1 and 3 in the european corn borer and determine tolerance to bacillus thuringiensis cry1ab toxin. | transgenic expression of bacillus thuringiensis (bt) crystalline (cry) toxins by crop plants result in reduced insect feeding damage, but sustainability is threatened by the development of resistance traits in target insect populations. we investigated bt toxin resistance trait in a laboratory colony of the european corn borer, ostrinia nubilalis, selected for increased survival when exposed to cry1ab and correlated survival on cry1ab toxin with a constitutive ∼146.2 ± 17.3-fold reduction in mid ... | 2013 | 24121099 |
| midgut aminopeptidase n isoforms from ostrinia nubilalis: activity characterization and differential binding to cry1ab and cry1fa proteins from bacillus thuringiensis. | aminopeptidase n (apn) isoforms from lepidoptera are known for their involvement in the mode of action of insecticidal cry proteins from bacillus thuringiensis. these enzymes belong to a protein family with at least eight different members that are expressed simultaneously in the midgut of lepidopteran larvae. here, we focus on the characterization of the apns from ostrinia nubilalis (onapns) to identify potential cry receptors. we expressed onapns in insect cells using a baculovirus system and ... | 2013 | 23933214 |
| shared midgut binding sites for cry1a.105, cry1aa, cry1ab, cry1ac and cry1fa proteins from bacillus thuringiensis in two important corn pests, ostrinia nubilalis and spodoptera frugiperda. | first generation of insect-protected transgenic corn (bt-corn) was based on the expression of cry1ab or cry1fa proteins. currently, the trend is the combination of two or more genes expressing proteins that bind to different targets. in addition to broadening the spectrum of action, this strategy helps to delay the evolution of resistance in exposed insect populations. one of such examples is the combination of cry1a.105 with cry1fa and cry2ab to control o. nubilalis and s. frugiperda. cry1a.105 ... | 2013 | 23861865 |
| quantitative genetic analysis of cry1ab tolerance in ostrinia nubilalis spanish populations. | tolerance to bacillus thuringiensis cry1ab toxin in spanish ostrinia nubilalis populations was analyzed by quantitative genetic techniques, using isolines established from field-derived insects. f1 offspring was tested for susceptibility to trypsin activated cry1ab using a concentration that caused a mean larval mortality of 87% (±17% sd). the progeny of the most tolerant isolines (that had shown mortalities lower than 60%) was crossed to obtain the f2 generation that was exposed to the same cry ... | 2013 | 23612057 |
| dispersal behavior of neonate european corn borer (lepidoptera: crambidae) on bt corn. | european corn borer, ostrinia nubilalis (hübner) (lepidoptera: crambidae), has historically been a significant economically important insect pest of corn (zea mays l.) in the united states and canada. the development in the 1990s of genetically modified corn expressing genes derived from bacillus thuringiensis (bt) that encodes insecticidal crystalline (cry) proteins has proven to be effective in controlling this insect as well as other corn pests. the purpose of this study was to assess the mov ... | 2012 | 22928300 |
| putting the brakes on a cycle: bottom-up effects damp cycle amplitude. | pest population density oscillations have a profound effect on agroecosystem functioning, particularly when pests cycle with epidemic persistence. here, we ask whether landscape-level manipulations can be used to restrict the cycle amplitude of the european corn borer moth [ostrinia nubilalis (hübner)], an economically important maize pest. we analysed time series from minnesota (1963-2009) and wisconsin (1964-2009) to quantify the extent of regime change in the us corn belt where rates of trans ... | 2012 | 22364256 |
| effects of intercropping with flowering plants on predation of ostrinia nubilalis (lepidoptera: crambidae) eggs by generalist predators in bell peppers. | bell pepper plots intercropped with flowering plants were measured for improving biological control provided by natural enemies of the european corn borer [ostrinia nubilalis (hübner)]. the intercropped plants dill, anethum graveolens l.; coriander, coriandrum sativum l.; and buckwheat, fagopyrum escuelentum moench; were established on the edge of two pepper plots and compared with nonintercropped control plots. predation by the three species orius insidiosus say; coleomegilla maculata degeer; a ... | 2012 | 22732620 |
| the influence of local factors on the prediction of fumonisin contamination in maize. | background: contamination by mycotoxins is a major concern to the maize industry in north-east italy where maize grain is often spoiled by fusarium spp. in this work, fumonisins, deoxynivalenol and zearalenone were determined and an artificial neural network (ann) model suitable for predicting mycotoxin contamination of maize at harvest time was developed. results: the occurrence of deoxynivalenol and zearalenone was very limited, while fumonisins concentration ranged from 163 and to 3663 µg kg( ... | 2012 | 22228027 |
| specific binding of radiolabeled cry1fa insecticidal protein from bacillus thuringiensis to midgut sites in lepidopteran species. | cry1fa insecticidal protein was successfully radiolabeled with (125)i-na. specific binding to brush border membrane vesicles was shown for the lepidopteran species ostrinia nubilalis, spodoptera frugiperda, spodoptera exigua, helicoverpa armigera, heliothis virescens, and plutella xylostella. homologous competition assays were performed to obtain equilibrium binding parameters (k(d) [dissociation constant] and r(t) [concentration of binding sites]) for these six insect species. | 2012 | 22447600 |
| characterization of cdnas encoding serine proteases and their transcriptional responses to cry1ab protoxin in the gut of ostrinia nubilalis larvae. | serine proteases, such as trypsin and chymotrypsin, are the primary digestive enzymes in lepidopteran larvae, and are also involved in bacillus thuringiensis (bt) protoxin activation and protoxin/toxin degradation. we isolated and sequenced 34 cdnas putatively encoding trypsins, chymotrypsins and their homologs from the european corn borer (ostrinia nubilalis) larval gut. our analyses of the cdna-deduced amino acid sequences indicated that 12 were putative trypsins, 12 were putative chymotrypsin ... | 2012 | 22952884 |
| field control of fusarium ear rot, ostrinia nubilalis (h++bner), and fumonisins in maize kernels. | a 6 year study was conducted to evaluate the possible support of pesticides in mitigating mycotoxin contamination in maize grown in northern italy. different pesticides to control ostrinia nubilalis (h++bner) (ecb), fusarium verticillioides (sacc.) nirenb. and aspergillus flavus link were considered to check their efficacy and the best schedule to reduce fumonisin and aflatoxin contamination. | 2011 | 21394878 |
| quantitative real-time pcr with sybr green detection to assess gene duplication in insects: study of gene dosage in drosophila melanogaster (diptera) and in ostrinia nubilalis (lepidoptera). | 2011 | 21443764 | |
| Identification of a novel aminopeptidase P-like gene (OnAPP) possibly involved in Bt toxicity and resistance in a major corn pest (Ostrinia nubilalis). | Studies to understand the Bacillus thuringiensis (Bt) resistance mechanism in European corn borer (ECB, Ostrinia nubilalis) suggest that resistance may be due to changes in the midgut-specific Bt toxin receptor. In this study, we identified 10 aminopeptidase-like genes, which have previously been identified as putative Bt toxin receptors in other insects and examined their expression in relation to Cry1Ab toxicity and resistance. Expression analysis for the 10 aminopeptidase-like genes revealed ... | 2011 | 21887358 |
| sex pheromone desaturase functioning in a primitive ostrinia moth is cryptically conserved in congeners' genomes. | (e)-11- and (z)-11-tetradecenyl acetate are the most common female sex pheromone components in ostrinia moths. the ?11-desaturase expressed in the pheromone gland (pg) of female moths is a key enzyme that introduces a double bond into pheromone molecules. a single ?11-desaturase of ostrinia nubilalis, onubz/e11, has been shown to produce an ~7:3 mixture of (e)-11- and (z)-11-tetradecenoate from the substrate tetradecanoate. in contrast, the sex pheromone of ostrinia latipennis, a primitive speci ... | 2011 | 21444802 |
| cross-resistance and mechanism of resistance to cry1ab toxin from bacillus thuringiensis in a field-derived strain of european corn borer, ostrinia nubilalis. | the cross-resistance spectrum and biochemical mechanism of resistance to the bacillus thuringiensis cry1ab toxin was studied in a field-derived strain of ostrinia nubilalis (hübner) (lepidoptera: crambidae) that was further selected in the laboratory for high levels (>1000-fold) of resistance to cry1ab. the resistant strain exhibited high levels of cross-resistance to cry1ac and cry1aa but only low levels of cross-resistance (<4-fold) to cry1f. in addition, there was no significant difference be ... | 2011 | 21550350 |
| a rearrangement of the z chromosome topology influences the sex-linked gene display in the european corn borer, ostrinia nubilalis. | males are homogametic (zz) and females are heterogametic (wz) with respect to the sex chromosomes in many species of butterflies and moths (insect order lepidoptera). genes on the z chromosome influence traits involved in larval development, environmental adaptation, and reproductive isolation. to facilitate the investigation of these traits across lepidoptera, we developed 43 degenerate primer pairs to pcr amplify orthologs of 43 bombyx mori z chromosome-linked genes. of the 34 orthologs that a ... | 2011 | 21573787 |
| cross-pollination of nontransgenic corn ears with transgenic bt corn: efficacy against lepidopteran pests and implications for resistance management. | the efficacy of nontransgenic sweet corn, zea mays l., hybrids cross-pollinated by bacillus thuringiensis (bt) sweet corn hybrids expressing cry1ab toxin was evaluated in both field and laboratory studies in minnesota in 2000. non-bt and bt hybrids (maternal plants) were cross-pollinated with pollen from both non-bt and bt hybrids (paternal plants) to create four crosses. subsequent crosses were evaluated for efficacy in the field against european corn borer, ostrinia nubilalis (hübner), and cor ... | 2011 | 22066174 |
| maize plants infestation by fusarium spp. and deoxynivalenol in genetically modified corn hybrid and traditional maize cultivars. | the objective of the performed investigations was to isolate pathogenic fungi from contaminated maize cobs, to assess the appearance of maize cob fusariosis and to determine grain contamination with deoxynivalenol in the cultivation of genetically modified maize containing a gene resistance against european corn borer (ostrinia nubilalis hbn) as well as selected non-modified cultivars. the plant material comprised the following genetically modified maize cultivar: dkc 3421 yg (mon 810) and non-m ... | 2011 | 22390066 |
| transcript analysis and comparative evaluation of shaker and slowmo gene homologues from the european corn borer, ostrinia nubilalis. | the movement and dispersal of larval lepidoptera impact their survival and distribution within the natural landscape. homologues of the drosophila behaviour-linked genes shaker (shkr) and slowmo (slmo) were identified from ostrinia nubilalis (lepidoptera: crambidae). onshkr was isolated as a 1610-nucleotide (nt) constitutively expressed transcript encoding a membrane-localized 469-amino-acid (aa) protein with a conserved tetramerization domain and the six-domain architecture necessary for the mo ... | 2011 | 21672063 |
| A physiologically based approach for degree-day calculation in pest phenology models: the case of the European Corn Borer (Ostrinia nubilalis Hbn.) in Northern Italy. | Phenological models based on degree-day accumulation have been developed to support the integrated pest management of many insects. Most of these models are based on linear relationships between temperature and development, and on daily time step simulations using daily minimum and maximum temperatures. This approach represents an approximation that does not take into account the insect physiological response to temperature, and daily temperature fluctuations. The objective of this work has been ... | 2011 | 21725633 |
| relationships of helicoverpa armigera, ostrinia nubilalis and fusarium verticillioides on mon 810 maize. | mon 810 maize was developed against ostrinia nubilalis and is suggested to indirectly decrease fusarium spp. infestation in maize ears. to evaluate this effect, co-occurrence of insect and fungal pests on mon 810 maize was studied. during 2009, exceptionally high maize ear infestation occurred in julianna-major (hungary). from investigation of some thousands of maize ears, the majority of the larval damage originated from helicoverpa armigera larvae, while o. nubilalis larvae contributed signifi ... | 2011 | 26467495 |
| Microbial diversity in the midguts of field and lab-reared populations of the European corn borer Ostrinia nubilalis. | BACKGROUND: Insects are associated with microorganisms that contribute to the digestion and processing of nutrients. The European Corn Borer (ECB) is a moth present world-wide, causing severe economical damage as a pest on corn and other crops. In the present work, we give a detailed view of the complexity of the microorganisms forming the ECB midgut microbiota with the objective of comparing the biodiversity of the midgut-associated microbiota and explore their potential as a source of genes an ... | 2011 | 21738787 |
| [incidence of microsporidian infection of stem borers of the genus ostrinia (lepidoptera: crambidae) in the krasnodar territory]. | infection of corn borers of the genus ostrinia with microsporidia is found for the first time in russia. incidence rates of the last instar larvae of the second generation, sampled under natural conditions in the krasnodar territory, is ranged between 3.0 and 17.2%. when infection incidence rates have been compared between larvae, collected (a) on the western and eastern borders of the region and (b) on dicotyledonous (cocklebur, mugwort) and monocotyledonous plants (maize), no distinct differen ... | 2011 | 21874854 |
| a single major qtl controls expression of larval cry1f resistance trait in ostrinia nubilalis (lepidoptera: crambidae) and is independent of midgut receptor genes. | the european corn borer, ostrinia nubilalis (lepidoptera: crambidae), is an introduced crop pest in north america that causes major damage to corn and reduces yield of food, feed, and biofuel materials. the cry1f toxin from bacillus thuringiensis (bt) expressed in transgenic hybrid corn is highly toxic to o. nubilalis larvae and effective in minimizing feeding damage. a laboratory colony of o. nubilalis was selected for high levels of cry1f resistance (>12,000-fold compared to susceptible larvae ... | 2011 | 21822602 |
| a multi-scale, landscape approach to predicting insect populations in agroecosystems. | landscape composition affects ecosystems services, including agricultural pest management. however, relationships between land use and agricultural insects are not well understood, and many complexities remain to be explored. here we examine whether nonagricultural landscapes can directly suppress agricultural pests, how multiple spatial scales of land use concurrently affect insect populations, and the relationships between regional land use and insect populations. we tracked densities of three ... | 2011 | 21830718 |
| bt maize and integrated pest management-a european perspective. | the european corn borer (ostrinia nubilalis), the mediterranean corn borer (sesamia nonagrioides) and the western corn rootworm (diabrotica virgifera virgifera) are the main arthropod pests in european maize production. practised pest control includes chemical control, biological control and cultural control such as ploughing and crop rotation. a pest control option that is available since 1996 is maize varieties that are genetically engineered (ge) to produce insecticidal compounds. ge maize va ... | 2011 | 21710684 |
| feeding behavior of neonate ostrinia nubilalis (lepidoptera: crambidae) on cry1ab bt corn: implications for resistance management. | the european corn borer, ostrinia nubilalis (h++bner) (lepidoptera: crambidae), is an economically important insect pest of corn, zea mays l., in the united states and canada. the development of genetically modified corn expressing genes derived from bacillus thuringiensis (bt) that encodes insecticidal crystalline (cry) proteins has proven to be effective in controlling this insect. to assess the feeding behavior of neonate o. nubilalis on bt corn, we examined differences in feeding behavior, b ... | 2011 | 21735897 |
| gene genealogies reveal differentiation at sex pheromone olfactory receptor loci in pheromone strains of the european corn borer, ostrinia nubilalis. | males of the e and z strains of the european corn borer ostrinia nubilalis (lepidoptera: crambidae) are attracted to different blends of the same pheromone components. the difference in male behavioral response is controlled by the sex-linked locus resp. the two types of males have identical neuroanatomy but their physiological specificity is reversed, suggesting that variation at the periphery results in behavioral change. differences in the olfactory receptors (ors) could explain the strain-sp ... | 2011 | 21644950 |
| efficacy of genetically modified bt toxins against insects with different genetic mechanisms of resistance. | transgenic crops that produce bacillus thuringiensis (bt) toxins are grown widely for pest control, but insect adaptation can reduce their efficacy. the genetically modified bt toxins cry1abmod and cry1acmod were designed to counter insect resistance to native bt toxins cry1ab and cry1ac. previous results suggested that the modified toxins would be effective only if resistance was linked with mutations in genes encoding toxin-binding cadherin proteins. here we report evidence from five major cro ... | 2011 | 21983521 |
| Changes in parasitoid communities over time and space: a historical case study of the maize pest Ostrinia nubilalis. | Understanding the ways in which human environmental modifications affect biodiversity is a key challenge in conservation planning, pest control and evolutionary ecology. Parasitoid communities, particularly those associated with agricultural pests, may be susceptible to such modifications. We document here changes in the larval parasitoid communities of Ostrinia nubilalis--the main pest of maize--and its sibling species O. scapulalis, based on two historical datasets, one collected from 1921-192 ... | 2011 | 21980436 |
| rapidly induced chemical defenses in maize stems and their effects on short-term growth of ostrinia nubilalis. | plants damaged by insect herbivory often respond by inducing a suite of defenses that can negatively affect an insect's growth and fecundity. ostrinia nubilalis (european corn borer, ecb) is one of the most devastating insect pests of maize, and in the current study, we examined the early biochemical changes that occur in maize stems in response to ecb herbivory and how these rapidly induced defenses influence the growth of ecb. we measured the quantities of known maize defense compounds, benzox ... | 2011 | 21833765 |
| sex-linked pheromone receptor genes of the european corn borer, ostrinia nubilalis, are in tandem arrays. | tuning of the olfactory system of male moths to conspecific female sex pheromones is crucial for correct species recognition; however, little is known about the genetic changes that drive speciation in this system. moths of the genus ostrinia are good models to elucidate this question, since significant differences in pheromone blends are observed within and among species. odorant receptors (ors) play a critical role in recognition of female sex pheromones; eight types of or genes expressed in m ... | 2011 | 21526121 |
| novel acidic sesquiterpenoids constitute a dominant class of pathogen-induced phytoalexins in maize. | nonvolatile terpenoid phytoalexins occur throughout the plant kingdom, but until recently were not known constituents of chemical defense in maize (zea mays). we describe a novel family of ubiquitous maize sesquiterpenoid phytoalexins, termed zealexins, which were discovered through characterization of fusarium graminearum-induced responses. zealexins accumulate to levels greater than 800 ++g g(-1) fresh weight in f. graminearum-infected tissue. their production is also elicited by a wide variet ... | 2011 | 21690302 |
| scanning the european corn borer (ostrinia spp.) genome for adaptive divergence between host-affiliated sibling species. | it has recently been shown that the european corn borer, a major pest of maize crops, is actually composed of two genetically differentiated and reproductively isolated taxa, which are found in sympatry over a wide geographical range in eurasia. each taxon is adapted to specific host plants: ostrinia nubilalis feeds mainly on maize, while o. scapulalis feeds mainly on hop or mugwort. here, we present a genome scan approach as a first step towards an integrated molecular analysis of the adaptive ... | 2011 | 21375617 |
| identity, regulation, and activity of inducible diterpenoid phytoalexins in maize. | phytoalexins constitute a broad category of pathogen- and insect-inducible biochemicals that locally protect plant tissues. because of their agronomic significance, maize and rice have been extensively investigated for their terpenoid-based defenses, which include insect-inducible monoterpene and sesquiterpene volatiles. rice also produces a complex array of pathogen-inducible diterpenoid phytoalexins. despite the demonstration of fungal-induced ent-kaur-15-ene production in maize over 30 y ago, ... | 2011 | 21402917 |
| the application and performance of single nucleotide polymorphism markers for population genetic analyses of lepidoptera. | microsatellite markers are difficult to apply within lepidopteran studies due to the lack of locus-specific pcr amplification and the high proportion of "null" alleles, such that erroneous estimations of population genetic parameters often result. herein single nucleotide polymorphism (snp) markers are developed from ostrinia nubilalis (lepidoptera: crambidae) using next generation expressed sequence tag (est) data. a total of 2742 snps were predicted within a reference assembly of 7414 est cont ... | 2011 | 22303334 |
| asian corn borer pheromone binding protein 3, a candidate for evolving specificity to the 12-tetradecenyl acetate sex pheromone. | most moth species in the genus ostrinia use varying ratios of (z)-11- and (e)-11-tetradecenyl acetate as their main sex pheromone components. the asian corn borer is unique within the genus having evolved to use pheromone components with a shift in the location of the double bond, (z)-12- and (e)-12-tetradecenyl acetate. we identified cdnas representing five pheromone binding proteins (pbps) and two sensory neuron membrane protein genes from an antennal transcriptome. the coding regions of the o ... | 2010 | 21056664 |
| genetic hitchhiking associated with life history divergence and colonization of north america in the european corn borer moth. | a primary goal for evolutionary biology is to reveal the genetic basis for adaptive evolution and reproductive isolation. using z and e pheromone strains the european corn borer (ecb) moth, i address this problem through multilocus analyses of dna polymorphism. i find that the locus triose phosphate isomerase (tpi) is a statistically significant outlier in coalescent simulations of demographic histories of population divergence, including strict allopatric isolation, restricted migration, second ... | 2010 | 21104111 |
| a delta 11 desaturase gene genealogy reveals two divergent allelic classes within the european corn borer (ostrinia nubilalis). | moth pheromone mating systems have been characterized at the molecular level, allowing evolutionary biologists to study how changes in protein sequence or gene expression affect pheromone phenotype, patterns of mating, and ultimately, the formation of barriers to gene exchange. recent studies of ostrinia pheromones have focused on the diversity of sex pheromone desaturases and their role in the specificity of pheromone production. here we produce a delta 11 desaturase genealogy within ostrinia n ... | 2010 | 20423501 |
| dispersal and movement behavior of neonate european corn borer (lepidoptera: crambidae) on non-bt and transgenic bt corn. | neonate movement and dispersal behavior of the european corn borer, ostrinia nubilalis (hübner) (lepidoptera: crambidae), were investigated under controlled conditions on bacillus thuringiensis (bt) and non-bt corn, zea mays l., to assess plant abandonment, dispersal from their natal plant, and silking behavior after bt and non-bt preexposure. with continuous airflow, neonates on a bt corn plant for 24 h abandoned that plant 1.78 times more frequently than neonates on a non-bt corn plant. indire ... | 2010 | 20429445 |
| planting patterns of in-field refuges observed for bt maize in minnesota. | the u.s. environmental protection agency (usepa) requires the use of nontransgenic refuges to slow the evolution of insect resistance to transgenic crops. in-field refuges, or refuges that are planted within the same field as the transgenic crop, are allowed; however, these refuges are required to be at least four rows wide. we described in-field planting patterns used by growers for both crylab [against ostrinia nubilalis (hübner)] and cry3bb (against diabrotica virgifera virgifera leconte) mai ... | 2010 | 20857753 |
| inheritance of central neuroanatomy and physiology related to pheromone preference in the male european corn borer. | the european corn borer (ecb), ostrinia nubilalis, is a textbook example of pheromone polymorphism. males of the two strains (z and e) prefer opposite ratios of the two pheromone components, z11- and e11-tetradecenyl acetate, with a sex-linked factor underlying this difference in preference. the male antennal lobes of the two strains contain a pheromone sensitive macroglomerular complex (mgc) that is identical in morphology, but reversed in functional topology. however, hybrids prefer intermedia ... | 2010 | 20846426 |
| fitness costs of cry1ab resistance in a field-derived strain of ostrinia nubilalis (lepidoptera: crambidae). | the study of fitness costs associated with resistance to toxins from bacillus thuringiensis berliner (bt) in ostrinia nubilalis (hübner) (lepidoptera: crambidae) is important for understanding resistance evolution and for evaluating resistance management practices that prevent or mitigate resistance to transgenic corn, zea mays l. resistant individuals identified from a field collection in kandiyohi, mn, were used to generate a cry1ab-resistant strain. we used susceptible and resistant strains w ... | 2010 | 20857752 |
| relevance of traditional integrated pest management (ipm) strategies for commercial corn producers in a transgenic agroecosystem: a bygone era? | the use of transgenic bt maize hybrids continues to increase significantly across the corn belt of the united states. in 2009, 59% of all maize planted in illinois was characterized as a "stacked" gene variety. this is a 40% increase since 2006. stacked hybrids typically express one cry protein for corn rootworm control and one cry protein for control of several lepidopteran pests; they also feature herbicide tolerance (to either glyphosate or glufosinate). slightly more than 50 years has passed ... | 2010 | 20873716 |
| areawide suppression of european corn borer with bt maize reaps savings to non-bt maize growers. | transgenic maize engineered to express insecticidal proteins from the bacterium bacillus thuringiensis (bt) has become widely adopted in u.s. agriculture. in 2009, bt maize was planted on more than 22.2 million hectares, constituting 63% of the u.s. crop. using statistical analysis of per capita growth rate estimates, we found that areawide suppression of the primary pest ostrinia nubilalis (european corn borer) is associated with bt maize use. cumulative benefits over 14 years are an estimated ... | 2010 | 20929774 |
| exposure and nontarget effects of transgenic bt corn debris in streams. | corn (zea mays l.) transformed with a gene from the bacterium bacillus thuringiensis (bt) comprises 49% of all corn in the united states. the input of senesced corn tissue expressing the bt gene may impact stream-inhabiting invertebrates that process plant debris, especially trichopteran species related to the target group of lepidopteran pests. our goal was to assess risk associated with transgenic corn debris entering streams. first, we show the input of corn tissue after harvest was extended ... | 2010 | 20388306 |
| a technique using reusable components for hand-infesting cornstalks with european corn borer (lepidoptera: crambidae) larvae. | field trials were conducted in 2005 and 2006 to evaluate the use of reusable wire nuts and nonreusable gelatin capsules for hand-infesting cornstalks with european corn borer, ostrinia nubilalis (hübner), (lepidoptera: crambidae) larvae. the reusable technique, which consists of a modified winggard plastic wire connector (i.e., wire nut) as a containment device for larvae, was compared over three plant growth stages to a gelatin capsule technique. in 2005 and 2006, the wire nut technique resulte ... | 2010 | 21309228 |
| managing resistance to bt crops in a genetically variable insect herbivore, ostrinia nubilalis. | to slow the resistance evolution of the european corn borer (ecb) to cry proteins expressed in transgenic bacillus thuringensis (bt) corn, the united states environmental protection agency (epa) has adopted an insect resistance management (irm) plan that relies on a "high dose/refuge" strategy. however, this irm plan does not consider possible ecological differences between the two ecb pheromone races (e and z). using carbon isotope analysis, we found that unstructured (non-corn) refuges contrib ... | 2010 | 20666246 |
| divergence in behaviour between the european corn borer, ostrinia nubilalis, and its sibling species ostrinia scapulalis: adaptation to human harvesting? | divergent adaptation to host plant species may be the major mechanism driving speciation and adaptive radiations in phytophagous insects. host plants can differ intrinsically in a number of attributes, but the role of natural enemies in host plant specialization is often underappreciated. here, we report behavioural divergence between the european corn borer (ecb, ostrinia nubilalis) and its sibling species ostrinia scapulalis, in relation to a major enemy: humans. harvesting maize imposes selec ... | 2010 | 20410041 |
| study of the aminopeptidase n gene family in the lepidopterans ostrinia nubilalis (hübner) and bombyx mori (l.): sequences, mapping and expression. | aminopeptidases n (apns) are a class of ectoenzymes present in lepidopteran larvae midguts, involved in the bacillus thuringiensis (bt) toxins mode of action. in the present work, seven aminopeptidases have been cloned from the midgut of ostrinia nubilalis, the major lepidopteran corn pest in the temperate climates. six sequences were identified as apns because of the presence of the hexxh(x)18e and gamen motifs, as well as the signal peptide and the gpi-anchor sequences. the remaining sequence ... | 2010 | 20420910 |
| allelic variation in a fatty-acyl reductase gene causes divergence in moth sex pheromones. | pheromone-based behaviours are crucial in animals from insects to mammals, and reproductive isolation is often based on pheromone differences. however, the genetic mechanisms by which pheromone signals change during the evolution of new species are largely unknown. in the sexual communication system of moths (insecta: lepidoptera), females emit a species-specific pheromone blend that attracts males over long distances. the european corn borer, ostrinia nubilalis, consists of two sex pheromone ra ... | 2010 | 20592730 |
| can a specialist parasitoid, macrocentrus cingulum (hymenoptera: braconidae), influence the ecotype structure of its preferred host ostrinia nubilalis (lepidoptera: crambidae)? | synchronization between a parasitoid and its preferred host is an essential strategy for successful biological control. two ecotypes of ostrinia nubilalis (hübner) (lepidoptera: crambidae) in north america are distinguished by their voltinism. in this study, the differential impact of a specialist parasitoid, macrocentrus cingulum brischke (hymenoptera: braconidae), on the univoltine and multivoltine populations of o. nubilalis is investigated. four years of field and laboratory study suggested ... | 2010 | 20429435 |
| a gut-specific chitinase gene essential for regulation of chitin content of peritrophic matrix and growth of ostrinia nubilalis larvae. | chitinases belong to a large and diverse family of hydrolytic enzymes that break down glycosidic bonds of chitin. however, very little is known about the function of chitinase genes in regulating the chitin content in peritrophic matrix (pm) of the midgut in insects. we identified a cdna putatively encoding a chitinase (oncht) in european corn borer (ecb; ostrinia nubilalis). the oncht transcript was predominately found in larval midgut but undetectable in eggs, pupae, or adults. when the larvae ... | 2010 | 20542114 |
| effects of infection with nosema pyrausta on survival and development of offspring of laboratory selected bt-resistant and bt-susceptible european corn borers. | infection with nosema pyrausta paillot lengthens developmental period of bt-susceptible ostrinia nubilalis (hübner) to a similar extent as feeding on cry1ab-incorporated diet in cry1ab-resistant o. nubilalis, and these two factors combined lengthen developmental period further than either alone. resistant o. nubilalis mating with infected susceptible, or infected resistant partners would produce partially- and fully-resistant offspring, respectively, infected with n. pyrausta. to investigate the ... | 2010 | 20673768 |
| journey in the ostrinia world: from pest to model in chemical ecology. | the european corn borer ostrinia nubilalis (ecb; lepidoptera: crambidae) is a widely recognized pest of agricultural significance over much of the northern hemisphere. because of the potential value of pheromone-based control, there has been considerable effort devoted to elucidation of the ecb chemical ecology. the species is polymorphic regarding its female-produced pheromone. partly because of this feature, over the years the ecb has become a model to study pheromone evolution. this review sh ... | 2010 | 20835755 |
| ostrinia revisited: evidence for sex linkage in european corn borer ostrinia nubilalis (hubner) pheromone reception. | the european corn borer, ostrinia nubilalis (hubner), is a keystone model for studies on the evolution of sex pheromone diversity and its role in establishing reproductive isolation. this species consists of two sympatric races, each utilizing opposite isomers of the same compound as their major pheromone component. female production and male response are congruent in each race, and males from each strain exhibit phenotypic differences in peripheral physiology. both strains possess co-localized ... | 2010 | 20846425 |
| variation in courtship ultrasounds of three ostrinia moths with different sex pheromones. | moths use ultrasounds as well as pheromones for sexual communication. in closely related moth species, variations in ultrasounds and pheromones are likely to profoundly affect mate recognition, reproductive isolation, and speciation. the european corn borer, ostrinia nubilalis, and its asian congeners, ostrinia furnacalis and ostrinia scapulalis, exhibit within-species and between-species variation in their pheromone communication. recently, we reported ultrasound communication in o. furnacalis; ... | 2010 | 20957230 |
| measurements of cry1f binding and activity of luminal gut proteases in susceptible and cry1f resistant ostrinia nubilalis larvae (lepidoptera: crambidae). | the biochemical mechanism of resistance to the bacillus thuringiensis cry1f toxin was studied in a laboratory-selected strain of ostrinia nubilalis (hübner) (lepidoptera: crambidae) showing more than 3000-fold resistance to cry1f and limited cross resistance to other cry toxins. analyses of cry1f binding to brush border membrane vesicles of midgut epithelia from susceptible and resistant larvae using ligand immunoblotting and surface plasmon resonance (spr) suggested that reduced binding of cry1 ... | 2010 | 19766122 |
| homology of olfactory receptor neuron response characteristics inferred from hybrids between asian and european corn borer moths (lepidoptera: crambidae). | first generation hybrid males from crosses between the asian corn borer (acb), ostrinia furnacalis, and the "univoltine z-strain" european corn borer (ecb), ostrinia nubilalis, were examined with respect to behavioral and physiological responses to acb and ecb pheromones. the hybrid males often flew to the pheromone of ecb z-strain, but very rarely to the acb pheromone. we mapped the tuning profiles of each orn of the f(1) hybrids with respect to the relevant pheromone components and a common be ... | 2010 | 19778540 |
| sex pheromone receptor specificity in the european corn borer moth, ostrinia nubilalis. | the european corn borer (ecb), ostrinia nubilalis (hubner), exists as two separate sex pheromone races. ecb(z) females produce a 97ratio3 blend of z11- and e11-tetradecenyl acetate whereas ecb(e) females produce an opposite 1ratio99 ratio of the z and e isomers. males of each race respond specifically to their conspecific female's blend. a closely related species, the asian corn borer (acb), o. furnacalis, uses a 3ratio2 blend of z12- and e12-tetradecenyl acetate, and is believed to have evolved ... | 2010 | 20084285 |
| isolation of bac clones containing conserved genes from libraries of three distantly related moths: a useful resource for comparative genomics of lepidoptera. | lepidoptera, butterflies and moths, is the second largest animal order and includes numerous agricultural pests. to facilitate comparative genomics in lepidoptera, we isolated bac clones containing conserved and putative single-copy genes from libraries of three pests, heliothis virescens, ostrinia nubilalis, and plutella xylostella, harboring the haploid chromosome number, n = 31, which are not closely related with each other or with the silkworm, bombyx mori, (n = 28), the sequenced model lepi ... | 2010 | 21127704 |
| characterization of six antibacterial response genes from the european corn borer (ostrinia nubilalis) larval gut and their expression in response to bacterial challenge. | six cdnas encoding putative antibacterial response proteins were identified and characterized from the larval gut of the european corn borer (ostrinia nubilalis). these antibacterial response proteins include four peptidoglycan recognition proteins (pgrps), one β-1,3-glucanase-1 (βglu-1), and one lysozyme. tissue-specific expression analysis showed that these genes were highly expressed in the midgut, except for lysozyme. analysis of expression of these genes in different developmental stage sho ... | 2010 | 21167833 |