Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| draft genome sequences of two bacillus thuringiensis strains and characterization of a putative 41.9-kda insecticidal toxin. | in this work, we report the genome sequencing of two bacillus thuringiensis strains using illumina next-generation sequencing technology (ngs). strain hu4-2, toxic to many lepidopteran pest species and to some mosquitoes, encoded genes for two insecticidal crystal (cry) proteins, cry1ia and cry9ea, and a vegetative insecticidal protein (vip) gene, vip3ca2. strain leapi01 contained genes coding for seven cry proteins (cry1aa, cry1ca, cry1da, cry2ab, cry9ea and two cry1ia gene variants) and a vip3 ... | 2014 | 24784323 |
| changes in gene expression in the larval gut of ostrinia nubilalis in response to bacillus thuringiensis cry1ab protoxin ingestion. | we developed a microarray based on 2895 unique transcripts assembled from 15,000 cdna sequences from the european corn borer (ostrinia nubilalis) larval gut. this microarray was used to monitor gene expression in early third-instar larvae of bacillus thuringiensis (bt)-susceptible o. nubilalis after 6 h feeding on diet, with or without the bt cry1ab protoxin. we identified 174 transcripts, for which the expression was changed more than two-fold in the gut of the larvae fed cry1ab protoxin (p < 0 ... | 2014 | 24704690 |
| using rna sequencing to characterize female reproductive genes between z and e strains of european corn borer moth (ostrinia nubilalis). | reproductive proteins often evolve rapidly and are thought to be subject to strong sexual selection, and thus may play a key role in reproductive isolation and species divergence. however, our knowledge of reproductive proteins has been largely limited to males and model organisms with sequenced genomes. with advances in sequencing technology, lepidoptera are emerging models for studies of sexual selection and speciation. by profiling the transcriptomes of the bursa copulatrix and bursal gland f ... | 2014 | 24621199 |
| understanding successful resistance management: the european corn borer and bt corn in the united states. | the european corn borer, ostrinia nubilalis hübner (lepidoptera: crambidae) has been a major pest of corn and other crops in north america since its accidental introduction nearly a hundred years ago. wide adoption of transgenic corn hybrids that express toxins from bacillus thuringiensis, referred to as bt corn, has suppressed corn borer populations and reduced the pest status of this insect in parts of the corn belt. continued suppression of this pest, however, will depend on managing potentia ... | 2014 | 22688691 |
| determination of areas with the most significant shift in persistence of pests in europe under climate change. | this study aimed to estimate the impact of climate change on the ranges of crop pest species in europe. the organisms included in the study were species from the family tortricidae (cydia pomonella, lobesia botrana) and the family pyralidae (ostrinia nubilalis), chrysomelidae beetles (leptinotarsa decemlineata, oulema melanopus) and species from the family aphididae (ropalosiphum padi, sitobion avenae). climate conditions in the year 2055 were simulated using a subset of five representative glob ... | 2014 | 23901033 |
| development and characterization of microsatellite markers for rice leaffolder, cnaphalocrocis medinalis (guenée) and cross-species amplification in other pyralididae. | rice leaffolder, cnaphalocrocis medinalis (guenée), is a destructive and widespread pest on rice. in this study, 20 microsatellite markers were isolated and characterized from c. medinalis partial genomic libraries using the method of fast isolation by aflp of sequence containing repeats. of these markers, 18 markers displayed polymorphisms. polymorphisms were evaluated in 48 individuals from two natural populations. the number of alleles per locus ranged from 2 to 15, and the expected and obser ... | 2014 | 24381106 |
| horizontal transfer of a non-autonomous helitron among insect and viral genomes. | the movement of mobile elements among species by horizontal transposon transfer (htt) influences the evolution of genomes through the modification of structure and function. helitrons are a relatively new lineage of dna-based (class ii) transposable elements (tes) that propagate by rolling-circle replication, and are capable of acquiring host dna. the rapid spread of helitrons among animal lineages by htt is facilitated by shuttling in viral particles or by unknown mechanisms mediated by close o ... | 2015 | 25766741 |
| linkage of an abcc transporter to a single qtl that controls ostrinia nubilalis larval resistance to the bacillus thuringiensis cry1fa toxin. | field evolved resistance of insect populations to bacillus thuringiensis (bt) crystalline (cry) toxins expressed by crop plants has resulted in reduced control of insect feeding damage to field crops, and threatens the sustainability of bt transgenic technologies. a single quantitative trait locus (qtl) that determines resistance in ostrinia nubilalis larvae capable of surviving on reproductive stage transgenic corn that express the bt cry1fa toxin was previously mapped to linkage group 12 (lg12 ... | 2015 | 26093031 |
| identification of a new cry1i-type gene as a candidate for gene pyramiding in corn to control ostrinia species larvae. | pyramiding of diverse cry toxin genes from bacillus thuringiensis with different modes of action is a desirable strategy to delay the evolution of resistance in the european corn borer (ostrinia nubilalis). considering the dependency of susceptibility to cry toxins on toxin binding to receptors in the midgut of target pests, a diverse mode of action is commonly defined as recognition of unique binding sites in the target insect. in this study, we present a novel cry1ie toxin gene (cry1ie2) as a ... | 2015 | 25795679 |
| aquatic degradation of cry1ab protein and decomposition dynamics of transgenic corn leaves under controlled conditions. | the increasing cultivation of genetically modified corn plants (zea mays) during the last decades is suggested as a potential risk to the environment. one of these genetically modified variety expressed the insecticidal cry1ab protein originating from bacillus thuringiensis (bt), resulting in resistance against ostrinia nubilalis, the european corn borer. transgenic litter material is extensively studied regarding the decomposition in soils. however, only a few field studies analyzed the fate of ... | 2015 | 25553417 |
| role of the european corn borer (ostrinia nubilalis) on contamination of maize with 13 fusarium mycotoxins. | the european corn borer (ecb) plays an important role in promoting fusarium verticillioides infections and in the consequent fumonisin contamination in maize grain in temperate areas. the objective of this study was to evaluate whether the ecb feeding activity could also affect the occurrence of emerging mycotoxins in maize kernels. during the 2008-10 period, natural infestation of the insect was compared, in field research, with the protection of infestation, which was obtained by using an ento ... | 2015 | 25266165 |
| redefinition of nosema pyrausta (perezia pyraustae paillot 1927) basing upon ultrastructural and molecular phylogenetic studies. | populations of european corn borer (ostrinia nubilalis hübner) from krasnodar territory (southwestern russia) become regularly infected with nosema-like microsporidia. to identify the parasite, it was subjected to electron microscopy and small subunit ribosomal rna (ssu rrna) gene sequencing. the spore ultrastructure of the parasite was highly similar to nosema bombycis from china and nosema pyrausta from the usa. the nucleotide sequence of ssu rrna gene was identical to a microsporidium isolate ... | 2015 | 25563606 |
| transcriptional analysis of susceptible and resistant european corn borer strains and their response to cry1f protoxin. | despite a number of recent reports of insect resistance to transgenic crops expressing insecticidal toxins from bacillus thuringiensis (bt), little is known about the mechanism of resistance to these toxins. the purpose of this study is to identify genes associated with the mechanism of cry1f toxin resistance in european corn borer (ostrinia nubilalis hübner). for this, we compared the global transcriptomic response of laboratory selected resistant and susceptible o. nubilalis strain to cry1f to ... | 2015 | 26220297 |
| effects of feeding transgenic corn with mcry1ac or maroacc gene to laying hens for 12 weeks on growth, egg quality and organ health. | the objective of the present study was to investigate the effect of feeding two transgenic corn lines containing the mcry1ac gene from bacillus thuringiensis strain (bt-799) and the maroacc gene from agrobacterium tumefaciens strain (cc-2), respectively, on growth, egg quality and organ health indicators. expression of the mcry1ac gene confers resistance to pyrausta nubilalis and the maroacc gene confers tolerance to herbicides. healthy hens (n=96 placed in cages; 3 hens/cage) were randomly assi ... | 2016 | 26915544 |
| cry1f resistance among lepidopteran pests: a model for improved resistance management? | the cry1fa protein from the bacterium bacillus thuringiensis (bt) is known for its potential to control lepidopteran pests, especially through transgenic expression in maize and cotton. the maize event tc1507 expressing the cry1fa toxin gene became commercially available in the united states in 2003 for the management of key lepidopteran pests including the european corn borer, ostrinia nubilalis, and the fall armyworm, spodoptera frugiperda. a high-dose/refuge strategy has been widely adopted t ... | 2016 | 27436741 |
| bacillus thuringiensis toxin resistance mechanisms among lepidoptera: progress on genomic approaches to uncover causal mutations in the european corn borer, ostrinia nubilalis. | transgenic plants that express bacillus thuringiensis (bt) crystal (cry) protein toxins (bt crops) effectively control feeding by the european corn borer, ostrinia nubilalis, although documented resistance evolution among a number of species in both the laboratory and field has heightened concerns about the durability of this technology. research has provided major insights into the mutations that alter bt toxin binding receptor structure and function within the midgut of lepidoptera that direct ... | 2016 | 27436734 |
| a high-throughput, in-vitro assay for bacillus thuringiensis insecticidal proteins. | a high-throughput, in-vitro assay for bacillus thuringiensis (bt) insecticidal proteins designated as cry was developed and evaluated for screening a large number of cry protein variants produced by dna shuffling. this automation-amenable assay exploits an insect cell line expressing a single receptor of bt cry proteins. the cry toxin used to develop this assay is a variant of the cry1ab protein called ip1-88, which was produced previously by dna shuffling. cell mortality caused by the activated ... | 2016 | 26524384 |
| additive effects on the improvement of insecticidal activity: design, synthesis, and insecticidal activity of novel pymetrozine derivatives. | a series of new pymetrozine analogues containing both methyl on the imine carbon and phenoxy group at the pyridine ring were designed and synthesized. their insecticidal activities against bean aphid (aphis craccivora), mosquito larvae (culex pipiens pallens), cotton bollworm (helicoverpa armigera), corn borer (ostrinia nubilalis) and oriental armyworm (mythimna separata) were evaluated. the results of bioassays indicated that most of the target compounds showed good insecticidal activity agains ... | 2016 | 26342545 |
| high regional genetic diversity and lack of host-specificity in ostrinia nubilalis (lepidoptera: crambidae) as revealed by mtdna variation. | the european corn borer (ostrinia nubilalis) infests a wide array of host plants and is considered one of the most serious pests of maize in europe. recent studies suggest that individuals feeding on maize in europe should be referred to o. nubilalis (sensu nov.), while those infesting dicots as ostrinia scapulalis (sensu nov.). we test if the clear genetic distinctiveness among individuals of o. nubilalis living on maize vs. dicots is tracked by mitochondrial dna (mtdna). we used fragments of c ... | 2016 | 27019346 |
| a fish-based chromosome map for the european corn borer yields insights into ancient chromosomal fusions in the silkworm. | a significant feature of the genomes of lepidoptera, butterflies and moths, is the high conservation of chromosome organization. recent remarkable progress in genome sequencing of lepidoptera has revealed that syntenic gene order is extensively conserved across phylogenetically distant species. the ancestral karyotype of lepidoptera is thought to be n=31; however, that of the most well-studied moth, bombyx mori, is n=28, and diverse studies suggest that three chromosomal fusion events occurred i ... | 2016 | 26264548 |
| semiautomated identification of european corn borer (lepidoptera: crambidae). | the european corn borer ostrinia nubilalis (hübner, 1796) is a serious and widely studied pest of corn. the most common method of its control is by means of insecticides. however, biological control is becoming more and more popular. the hymenopteran parasitoid trichogramma sp. is the most promising and effective one among the biological agents and is now widely used in north america and europe. its application should occur at the time when the european corn borer is at the beginning of the eggs ... | 2016 | 26487742 |
| novel hydrazone derivatives containing pyridine amide moiety: design, synthesis, and insecticidal activity. | a series of novel hydrazone derivatives containing pyridine amide moiety were designed, synthesized, and evaluated for their insecticidal activity. bioassays indicated that some of the target compounds exhibited good insecticidal activities against nilaparvata lugens (n. lugens), plutella xylostella (p. xylostella), mythimna separata (m. separata), helicoverpa armigera (h. armigera), pyrausta nubilalis (p. nubilalis), and culex pipiens pallens (c. pipiens pallens). in particular, compound 5j rev ... | 2016 | 26832221 |
| synthesis and antiviral, insecticidal, and fungicidal activities of gossypol derivatives containing alkylimine, oxime or hydrazine moiety. | gossypol is a part of the cotton plant's defense system against pathogens and herbivorous insects. to discover gossypol analogs with broad spectrum and high activity, a series of gossypol alkylamine schiff base, oxime and hydrazone derivatives were synthesised and bioassayed. the biological results indicated that most of these derivatives exhibited higher anti-tmv activity than gossypol. interestingly, the activities of compounds 10, 15, 18, 20, 23 and 26 were much higher than that of ribavirin. ... | 2016 | 26337022 |
| various bioactivity and relationship of structure-activity of matrine analogues. | for the first time, the botanic source natural product matrine was reported to have more potent inhibitory activity against tobacco mosaic virus (tmv) than the commercial virucide ribavirin. on the basis of the structural diversity modification strategy, a series of matrine derivatives was synthesized and systematically evaluated for their antiviral activity against tmv, fungicidal activity, and insecticidal activity. as a result, compounds 3 (inhibitory rate 67.3%, 69.5%, 63.7%, 63.0% at 500 μg ... | 2017 | 28248103 |
| comparisons of transcriptional profiles of gut genes between cry1ab-resistant and susceptible strains of ostrinia nubilalis revealed genes possibly related to the adaptation of resistant larvae to transgenic cry1ab corn. | a microarray developed on the basis of 2895 unique transcripts from larval gut was used to compare gut gene expression profiles between a laboratory-selected cry1ab-resistant (r) strain and its isoline susceptible (s) strain of the european corn borer (ostrinia nubilalis) after the larvae were fed the leaves of transgenic corn (mon810) expressing cry1ab or its non-transgenic isoline for 6 h. we revealed 398 gut genes differentially expressed (i.e., either up- or down-regulated genes with express ... | 2017 | 28146087 |
| insecticidal spectrum and mode of action of the bacillus thuringiensis vip3ca insecticidal protein. | the vip3ca protein, discovered in a screening of spanish collections of bacillus thuringiensis, was known to be toxic to chrysodeixis chalcites, mamestra brassicae and trichoplusia ni. in the present study, its activity has been tested with additional insect species and we found that cydia pomonella is moderately susceptible to this protein. vip3ca (of approximately 90kda) was processed to an approximately 70kda protein when incubated with midgut juice in all tested species. the kinetics of prot ... | 2017 | 27756652 |