Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| bovine rotavirus in turkeys with enteritis. | rotaviruses are the main agents responsible for diarrhea in different animal species and for infantile gastroenteritis. these viruses have been isolated from various avian species and have often been associated with poult enteritis and mortality syndrome. nevertheless, the knowledge of rotavirus infection in turkeys is scarce. six group a rotavirus strains obtained from pooled enteric contents of diarrheic turkeys were isolated in ma-104 cell culture and typed as g(6)p(1), a typical bovine rotav ... | 2011 | 22312995 |
| viral and bacterial infections associated with camel (camelus dromedarius) calf diarrhea in north province, saudi arabia. | diarrhea and deaths in new-born camel calves were noticed by veterinary investigators and pastoralist in saudi arabia to be very high. hence, it is thought to be necessary to investigate this problem from the virological and bacteriological point of view. the role of pathogenic bacteria and viruses in six different towns of north province (al-assafia, arar, domat aljandal, hail, skaka and khoa) in saudi arabia was studied. survey was conducted in diarrheic camel calves aged 12 months or younger. ... | 2011 | 23961160 |
| instability of human rotavirus g genotypes circulating in a rural area of bangladesh. | a total of 280 fecal specimens from patients with acute gastroenteritis attending one rural hospital (dharmapasha health complex, sunamgonj) in bangladesh from august 2004 to may 2006 were tested for rotavirus by polyacrylamide gel electrophoresis (page). the diversity of rotavirus was investigated using electropherotyping and reverse transcription-pcr amplification of the vp7 and vp4 genes. the electrophoretic patterns of dsrna of rotavirus showed 9 different migrations (6 long and 3 short) by ... | 2011 | 21240155 |
| vp7 gene of human rotavirus a genotype g5: phylogenetic analysis reveals the existence of three different lineages worldwide. | group a rotavirus (rv-a) genotype g5, which is common in pigs, was also detected in children with severe diarrhea in brazil, argentina, paraguay, cameroon, china, thailand, and vietnam. to evaluate the evolutionary relationship among rv-a g5 strains, the vp7 and vp4 genes of 28 brazilian rv-a g5 human strains, sampled between 1986 and 2005, were sequenced and compared with other rv-a g5 strains currently circulating worldwide in animals and humans. the phylogenetic analysis of rv-a g5 vp7 gene s ... | 2011 | 21181934 |
| full genomic analysis of a g8p[1] rotavirus strain isolated from an asymptomatic infant in kenya provides evidence for an artiodactyl-to-human interspecies transmission event. | group a rotavirus (gar) g8p[1] strains, found sometimes in cattle, have been reported rarely from humans. therefore, analysis of the full genomes of human g8p[1] strains are of significance in the context of studies on interspecies transmission of rotaviruses. however, to date, only partial-length nucleotide sequences are available for the 11 genes of a single human g8p[1] strain, while the partial sequences of two other strains have been reported. the present study reports the first complete ge ... | 2011 | 21181935 |
| a waterborne outbreak of epidemic diarrhea due to group a rotavirus in malatya, turkey. | we characterized an outbreak of acute diarrheal disease caused by group a rotavirus that occurred during the autumn of 2005 in malatya city, turkey. a total 9907 patients between 0 to 91 years old (mean age: 25.05?19.67) were included in the epidemic. the patients? data were prospectively collected and statistically analyzed. microbiologic analyses were performed to determine the etiologic agent. rapid onset diarrhea (98.36%), abdominal cramps (69%), fever (44.4%) and vomiting (69.6%) were the m ... | 2011 | 21344142 |
| comparing electron microscopy and a competitive blocking elisa in the detection of rotaviruses in porcine faeces. | analysis of 476 faecal samples from diarrhoeic piglets was performed using electron microscopy (em) and a competitive blocking (cb)-elisa based on monoclonal antibodies to the vp6 protein of group a rotavirus. rotavirus was detected by em and/or cb-elisa in 111 (23.3%) samples. of these, all groups of rotavirus were identified in 83 (74.8%) samples by em (em+), while group a rotavirus was identified in 90 (81.1%) samples by cb-elisa (elisa+). however, only 62 (55.9%) of samples were positive usi ... | 2011 | 20036588 |
| laboratory-based rotavirus surveillance during the introduction of a vaccination program, brazil, 2005-2009. | : brazil introduced universal antirotavirus vaccination in march 2006. this article reports the results of rotavirus a (rv-a) surveillance from january 2005 to december 2009. | 2011 | 21048523 |
| rotavirus a genotype p[4]g2: genetic diversity and reassortment events among strains circulating in brazil between 2005 and 2009. | group a rotaviruses (rv-a) are the leading cause of severe gastroenteritis in infants and young children worldwide. due to the epidemiologic complexity of rv-a, especially in developing countries, it is important to determine which genotypes are circulating, principally after the introduction in march 2006 of the monovalent (p[8]g1) rotarix® vaccine in brazil by the national immunization program. in phase iii trials with rotarix®, the prevalence of genotype p[4]g2 was extremely low, and therefor ... | 2011 | 21503926 |
| analysis of human p[4]g2 rotavirus strains isolated in brazil reveals codon usage bias and strong compositional constraints. | the rotavirus genus belongs to the family reoviridae and its genome consist of 11 segments of double-stranded rna. group a rotaviruses (rv-a) are the main etiological agent of acute viral gastroenteritis in infants and young children worldwide. understanding the extent and causes of biases in codon usage is essential to the understanding of viral evolution. however, the factors shaping synonymous codon usage bias and nucleotide composition in human rv-a are currently unknown. in order to gain in ... | 2011 | 21255687 |
| development of a combined immunomagnetic separation and quantitative reverse transcription-pcr assay for sensitive detection of infectious rotavirus in water samples. | a quantitative and rapid detection method for rotavirus in water samples was developed using immunomagnetic separation combined with quantitative reverse transcription-polymerase chain reaction (ims-rt-qpcr). magnetic beads coated with antibodies against representative group a rotavirus were used to capture and purify intact rotavirus particles in both artificial and real environmental water sample matrix. compared to extracting rna using commercial kits and rt-qpcr assay, the developed ims-rt-q ... | 2011 | 21256895 |
| highly sensitive detection of the group a rotavirus using apolipoprotein h-coated elisa plates compared to quantitative real-time pcr. | the principle of a capture elisa is binding of specific capture antibodies (polyclonal or monoclonal) to the surface of a suitable 96 well plate. these immobilized antibodies are capable of specifically binding a virus present in a clinical sample. subsequently, the captured virus is detected using a specific detection antibody. the drawback of this method is that a capture elisa can only function for a single virus captured by the primary antibody. human apolipoprotein h (apoh) or ß2-glycoprote ... | 2011 | 21310042 |
| reassortment among bovine, porcine and human rotavirus strains results in g8p[7] and g6p[7] strains isolated from cattle in south korea. | group a rotaviruses (garvs) cause severe acute gastroenteritis in children and young animals. although zoonotic infections with bovine-like g6 and g8 garvs have been reported in many countries, there is little evidence for reassortment between bovine garvs and garvs from heterologous species. the finding of bovine garvs with the g6 and g8 genotypes in combination with the typical porcine p[7] prompted us to characterize all 11 genes of 30 bovine garvs isolated from clinically infected calves. by ... | 2011 | 21592683 |
| a community outbreak of rotavirus diarrhea associated with exposures in a hospital outpatient department in south china. | introduction:: between october 1 and december 10, 2006, bao'an, china had a 5-fold increase in acute rotavirus gastroenteritis (arge) cases compared with the same time in 2005. to identify the risk factors for arge during this outbreak, we conducted a case-control study among children ≤24 months old from the most heavily affected area. methods:: we defined an arge case as diarrhea, with group a rotavirus antigen detected from fecal samples by the enzyme-linked immunosorbent assay test. in the ca ... | 2011 | 21587095 |
| norovirus infections in children under 5 years of age hospitalized due to the acute viral gastroenteritis in northeastern poland. | the primary aim of this study was to evaluate the frequency and seasonality of norovirus infection in hospitalized polish children under 5 years of age, and a secondary aim was to compare the clinical severity of norovirus and rotavirus disease. the prospective surveillance study was carried out from july 2009 through june 2010. stool samples from 242 children hospitalized due to acute viral gastroenteritis were tested for rotavirus group a and adenovirus with commercial immunochromatographic te ... | 2011 | 21732202 |
| detection and full genomic analysis of g6p[9] human rotavirus in japan. | a rare genotype g6p[9] was identified in two human group a rotavirus strains designated as kf14 and kf17, that were detected in stool specimens from children with diarrhea in japan. vp7 gene sequences of these two strains were identical and genetically closely related to g6 human rotavirus strains reported in european countries and the united states. to our knowledge, this is the first report of detection of a g6 human rotavirus in japan. for further genetic analysis to elucidate the origin of t ... | 2011 | 21656185 |
| phylogenetic analysis of rotavirus a nsp2 gene sequences and evidence of intragenic recombination. | the rotavirus non-structural protein nsp2 is one of the earliest and most abundant viral proteins produced during infection. this protein has multiple essential roles in the replication cycle involving rna binding, viroplasm formation, helicase and can hydrolyse the +¦-phosphate of rna and ntps acting as an rtpase and ntpase. in studying sequences from rotavirus strains isolated in australia between 1984 and 2009, the nsp2 gene was seen to be highly conserved and clustered with defined nsp2 geno ... | 2011 | 21689784 |
| [annual incidence of human group a rotavirus g-serotypes detected in a japanese university hospital between 2003 and 2007]. | group a rotavirus g-serotyping by polymerase chain reaction (pcr) using university hospital subject samples in september 2003 to august 2004, september 2004 to august 2005, september 2005 to august 2006, and september 2006 to august 2007 showed the most common serotypes g1 and g3, detected in 27 and 33 subjects, compared to 4 subjects in whom serotype g4 was detected. between 2003 and 2004, serotypes g1 accounted for 50% and g3 for 38%, contrasting with serotype g3 at 79% between 2004 and 2005, ... | 2011 | 21404601 |
| intestinal and extra-intestinal pathogenicity of a bovine reassortant rotavirus in calves and piglets. | despite the impact of bovine group a rotaviruses (garvs) as economically important and zoonotic pathogens, there is a scarcity of data on cross-species pathogenicity and extra-intestinal spread of bovine reassortant garvs. during the course of characterizing the genotypes of all 11 genomic segments of bovine garvs isolated from diarrheic calves in south korea, a unique g6p[7] reassortant garv strain (kj9-1) was isolated. the strain harbors five bovine-like gene segments (vp7: g6; vp6: i2; vp1: r ... | 2011 | 21658866 |
| genetic analyses reveal differences in the vp7 and vp4 antigenic epitopes between human rotaviruses circulating in belgium and rotaviruses in rotarix™ and rotateq™ | two live-attenuated rotavirus group a (rva) vaccines, rotarix™ (g1p[8]) and rotateq™ (g1-g4, p[8]), have been successfully introduced in many countries worldwide, including belgium. the parental rva strains used to generate the vaccines were isolated more than 20 years ago in france (g4 parental strain in rotateq™) and the united states (all other parental strains). at present, little is known about the relationship between currently circulating human rvas and the vaccine strains. in this study, ... | 2011 | 22189107 |
| uniformity of rotavirus strain nomenclature proposed by the rotavirus classification working group (rcwg). | in april 2008, a nucleotide-sequence-based, complete genome classification system was developed for group a rotaviruses (rvs). this system assigns a specific genotype to each of the 11 genome segments of a particular rv strain according to established nucleotide percent cutoff values. using this approach, the genome of individual rv strains are given the complete descriptor of gx-p[x]-ix-rx-cx-mx-ax-nx-tx-ex-hx. the rotavirus classification working group (rcwg) was formed by scientists in the fi ... | 2011 | 21597953 |
| high prevalence of enteric viruses in untreated individual drinking water sources and surface water in slovenia. | waterborne infections have been shown to be important in outbreaks of gastroenteritis throughout the world. although improved sanitary conditions are being progressively applied, fecal contaminations remain an emerging problem also in developed countries. the aim of our study was to investigate the prevalence of fecal contaminated water sources in slovenia, including surface waters and groundwater sources throughout the country. in total, 152 water samples were investigated, of which 72 samples ... | 2011 | 21665537 |
| multiple reassortment and interspecies transmission events contribute to the diversity of feline, canine and feline/canine-like human group a rotavirus strains. | rna-rna hybridization assays and complete genome sequence analyses have shown that feline rotavirus (frv) and canine rotavirus (crv) strains display at least two distinct genotype constellations (genogroups), represented by the frv strain rva/cat-tc/aus/cat97/1984/g3p[3] and the human rotavirus (hrv) strain rva/human-tc/jpn/au-1/1982/g3p3[9], respectively. g3p[3] and g3p[9] strains have been detected sporadically in humans. the complete genomes of two crv strains (rva/dog-tc/ita/rv198-95/1995/g3 ... | 2011 | 21609783 |
| grazing management effects on sediment, phosphorus, and pathogen loading of streams in cool-season grass pastures. | erosion and runoff from pastures may lead to degradation of surface water. a 2-yr grazing study was conducted to quantify the effects of grazing management on sediment, phosphorus (p), and pathogen loading of streams in cool-season grass pastures. six adjoining 12.1-ha pastures bisected by a stream in central iowa were divided into three treatments: continuous stocking with unrestricted stream access (csu), continuous stocking with restricted stream access (csr), and rotational stocking (rs). ra ... | 2011 | 21712600 |
| experimental infection of pigs with group a rotavirus and enterotoxigenic escherichia coli in india: gross, histopathological and immunopathological study. | the authors describe a detailed study conducted in assam, india, of gross, histopathological and immunopathological alterations in pigs experimentally infected with rotavirus and enterotoxigenic escherichia coli (etec) expressing k88 pili. a total of 30 caesarean derived piglets were infected experimentally with rotavirus alone or in combination with etec to study the gross and histopathological alterations and the distribution pattern of different b- and t cell subsets in the gut. villus atroph ... | 2011 | 21706463 |
| genetic heterogeneity of wild-type g4p[6] porcine rotavirus strains detected in a diarrhea outbreak in a regularly vaccinated pig herd. | group a rotavirus (rv-a) with short electropherotype was identified by ss-page in a neonatal diarrhea outbreak at a brazilian pig farm where the sows were regularly vaccinated with a commercial vaccine containing osu (g5p[7]) and gottfried (g4p[6]) porcine rv-a (porv-a) strains. the ss-page positive stool samples (n=20) were characterized as p[6] genotype by multiplex-nested-rt-pcr assay. the nucleotide analysis of the vp4 gene (vp8*) state that the viruses clustered in p[6] lineages that are al ... | 2011 | 21775076 |
| phylogenetic analysis of vp1, vp2, and vp3 gene segments of genotype g5 group a rotavirus strains circulating in brazil between 1986 and 2005. | genotype g5 group a rotavirus (rv-a), which is common in pigs and also detected in horses and cattle, circulated as endemic genotype in the 1980s and early 1990s in brazil. after 1996, g5 rv-a has been replaced by g9 rv-a, becoming only sporadically detected. recently, g5 has been reported in children with severe diarrhea in argentina, cameroon, paraguay, people's republic of china, and vietnam, suggesting that, although uncommon in humans, it has a worldwide distribution. in a previous study, b ... | 2011 | 21827799 |
| incidence assessment of rotavirus and adenovirus associated acute gastroenteritis cases in early childhood. | this study was undertaken to give an insight into the incidence of acute gastroenteritis cases of rotaviral/adenoviral aetiology in patients presenting to the emergency room of an inner-city government teaching hospital. group a rotavirus and adenovirus serotype 40-41 antigen results were obtained via immunochromatography. in 2007, there were 1543 patients with gastroenteritis between 0-5 years of age whose stool samples were tested for rota and adenovirus, of whom 386 (25%) had positive stool s ... | 2011 | 21753251 |
| monitoring of group c rotavirus in children with acute gastroenteritis in brazil: an emergent epidemiological issue after rotavirus vaccine? | group c rotavirus (gpcrv) has a worldwide distribution; however, its epidemiology and ecology are still unclear. evidence for a possible zoonotic role has been postulated recently for brazilian children strains. the aim of this study was to monitor gpcrv in children ôëñ15 years with acute gastroenteritis during the 2007-2010 national brazilian rotavirus surveillance, and to undertake the molecular characterization of the major vp6 capsid protein. a total of 3,019 fecal samples were first screene ... | 2011 | 21739455 |
| genotype diversity of group a rotavirus strains in children with acute diarrhea in urban burkina faso, 2008-2010. | in this study, the diversity of g and p genotypes of rotavirus strains in burkinabe children were examined. between november 2008 and february 2010, 447 stool samples were collected from children <5 years of age with acute diarrhea visiting hospital in ouagadougou. group a rotavirus was previously detected in 151/447 (33.8%) of the samples tested by an immunochromatographic test and these samples were now tested further for rotavirus g and p genotypes by rt-pcr. of these, the rotavirus type gene ... | 2011 | 21678452 |
| complications in hospitalized children with acute gastroenteritis caused by rotavirus: a retrospective analysis. | acute gastroenteritis (age) caused by rotavirus (rv) is a common disease among infants and toddlers, often leading to hospitalization and, in resource-poor countries, to death. however, little is known on specific complications of severe rv-positive (rv+) age and on the clinical course in chronically ill children. this was a retrospective analysis of data for children <5-áyears of age hospitalized due to age during six rotavirus seasons in three large german pediatric hospitals. the primary stud ... | 2011 | 21833497 |
| Molecular epidemiology and clinical characterization of group A rotavirus infections in Tunisian children with acute gastroenteritis. | Rotaviruses are the most common cause of severe viral gastroenteritis in early childhood worldwide. Thus, the objectives of our study were to determine the molecular epidemiology and the clinical features of rotavirus gastroenteritis in Tunisia. Between January 2003 and April 2007, a prospective study was conducted on 788 stool samples collected from children under 12 years of age who were suffering from acute gastroenteritis. Rotavirus was detected by multiplex RT-PCR in 27% (n = 213) of sample ... | 2011 | 21942357 |
| suspected zoonotic transmission of rotavirus group a in danish adults. | summarygroup a rotaviruses infect humans and a variety of animals. in july 2006 a rare rotavirus strain with g8p[14] specificity was identified in the stool samples of two adult patients with diarrheoa, who lived in the same geographical area in denmark. nucleotide sequences of the vp7, vp4, vp6, and nsp4 genes of the identified strains were identical. phylogenetic analyses showed that both danish g8p[14] strains clustered with rotaviruses of animal, mainly, bovine and caprine, origin. the high ... | 2011 | 21943834 |
| [Detection and genotyping of rotavirus in diarrhea infant feces in Chengdu]. | To study the genotype of rotavirus and the genetic variations of the major neutralization antigen VP4 of group A rotavirus in fecal samples from infants with diarrhea in Chengdu, Sichuan province, China. | 2011 | 21866637 |
| genetic diversity of group a rotaviruses associated with repeated outbreaks of diarrhea in a farrow-to-finish farm: identification of a porcine rotavirus strain bearing a novel vp7 genotype, g26. | abstract: group a rotaviruses (gars) are one of the most common causes of diarrhea in suckling pigs. although a number of g and p genotypes have been identified in porcine gars, few attempts have been made to study the molecular epidemiology of these viruses associated with diarrhea outbreaks within a farm over an extended period of time. here, we investigated the molecular characteristics of gars that caused four outbreaks of diarrhea among suckling pigs in a farrow-to-finish farm over the cour ... | 2011 | 22067072 |
| First description of group A rotavirus from fecal samples of ostriches (Struthio camelus). | This study investigated the occurrence of rotavirus infections in ostriches (Struthio camelus) reared in Northern Paraná, Brazil. Fecal (n=66) and serum (n=182) samples from nine farms located in four different cities were analyzed by silver stained-polyacrylamide gel electrophoresis (ss-PAGE), RT-PCR assay, virus isolation, and counterimmunoelectroosmophoresis (CIE). Rotavirus group A seropositivity occurred in 5.49% (10/182) of serum samples of ostriches originated from two farms. Only 9.09% ( ... | 2011 | 22209018 |
| human rotavirus genotypes detection among hospitalized children, a study in tehran, iran. | human rotavirus genotypes g1-g4, g9, p[4] and p[8] are major worldwide causes of acute gastroenteritis in children. rotavirus genotype g1p[8] is predominant in many countries. in this study, the genotypic diversity of group a rotaviruses were detected in children <5 years of age who were treated for dehydration and diarrhea in tehran, iran from october 2004 to september 2008. | 2011 | 21194260 |
| outbreak of rotavirus gastroenteritis in a nursing home, slovenia, december 2010. | a gastroenteritis outbreak affected 45 people (40 residents and five staff) in a nursing home for the elderly in the celje region, north-east slovenia, between 17 december and 31 december 2010. rotavirus group a was laboratory confirmed in the stools of five ill individuals. the outbreak was identified when the number of affected persons was high but was successfully controlled after implementing preventive measures. | 2011 | 21492527 |
| detection of group a rotavirus rna and antigens in serum and cerebrospinal fluid from two children with clinically mild encephalopathy with a reversible splenial lesion. | we report on two children with mild encephalopathy with a reversible splenial lesion associated with group a rotavirus (garv) infection. we examined stool, serum, and cerebrospinal fluid samples to determine the presence of the garv vp7 gene and garv antigen by reverse-transcription pcr and enzyme-linked immunosorbent assay, respectively. garv antigen was detected in stool samples from both patients. the garv g genotype was g9 in one child and g3 in the other. garv antigens were also found in bo ... | 2011 | 21617303 |
| detection of the first g6p[14] human rotavirus strain from a child with diarrhea in egypt. | we report the first detection of a g6p[14] rotavirus strain in egypt from the stool of a child participating in a hospital-based diarrhea surveillance study conducted throughout the year 2004. rotavirus infection was initially detected using a rotavirus group a vp6 enzyme immunoassay; the p (vp4) and g (vp7) genotypes of the strain were identified by rt-pcr. we sequenced the vp7 gene and the vp8* portion of the vp4 gene and the strain displayed the strongest identity to the vp7 [>94% nucleotides ... | 2011 | 21640199 |
| a single-tube multiplex pcr for rapid detection in feces of 10 viruses causing diarrhea. | a novel multiplex polymerase chain reaction assay was developed to identify 10 viruses in a single tube. the assay was targeted to detect group a and c rotaviruses, adenovirus, norovirus gi, norovirus gii, sapovirus, astrovirus, aichi virus, parechovirus, and enterovirus. a total of 235 stool samples were collected from infants and children with acute gastroenteritis in kyoto, japan, from 2008 to 2009, then tested by this novel multiplex pcr and compared with a multiplex pcr described previously ... | 2011 | 21349292 |
| burden and typing of rotavirus group a in latin america and the caribbean: systematic review and meta-analysis. | the efficacy of licensed rotavirus vaccines has only been shown against certain rotavirus group a (rv-a) types. it is critical to understand the burden of rotavirus gastroenteritis (rvge) and its prevalent types to assess the potential impact of these vaccines in latin america and the caribbean (la&c). we performed a systematic review and meta-analyses of all the available evidence reported from 1990 to 2009 on the burden of rotavirus disease and strains circulating in la&c. eligible studies-185 ... | 2011 | 21384462 |
| molecular characterization of group a rotaviruses detected in children with gastroenteritis in ireland in 2006-2009. | summarycommunity and hospital-acquired cases of human rotavirus are responsible for millions of gastroenteritis cases in children worldwide, chiefly in developing countries, and vaccines are now available. during surveillance activity for human rotavirus infections in ireland, between 2006 and 2009, a total of 420 rotavirus strains were collected and analysed. upon either pcr genotyping and sequence analysis, a variety of vp7 (g1-g4 and g9) and vp4 (p[4], p[6], p[8] and p[9]) genotypes were dete ... | 2011 | 21396145 |
| monitoring of group a rotaviruses in wild-living birds in hungary. | rotavirus is a common pathogen causing gastroenteritis in humans and domesticated animals. the incidence of rotavirus in wild-living animals, particularly in avian species, has not been systematically investigated. in this study 1220 fecal samples and cloacal swabs collected from wild-living birds during 2008 in hungary were tested for the presence of group a rotaviruses by a vp6 gene-specific reverse-transcription-polymerase-chain-reaction assay. of the 1220 samples, 276 and 944 were processed ... | 2011 | 21500648 |
| identification of an antibody binding epitope on the rotavirus non-structural protein nsp2 using phage display analysis. | the nsp2 of rotavirus has important roles in rotavirus replication associated with rna binding, hydrolysis of ntps and rna, and helix destabilising properties. a cell culture assay using a nsp2 specific monoclonal antibody (mab) and polyclonal antiserum to block viral replication showed a 73% and a 96% reduction in the amount of virus produced during replication respectively. phage display technology was used to identify the antibody binding region on the nsp2 protein with the motif (244)t-(y/f) ... | 2011 | 21697352 |
| co-circulation of g1, g2 and g9 rotaviruses in hospitalized patients in bangladesh during 2006-2009. | rotavirus strain diversity in bangladesh has been explored since 1985 and as seen in other parts of the world, rotaviruses have shown tremendous strain diversity overtime. rotavirus antigen was detected in stool specimens using a solid-phase sandwich-type enzyme immunoassay. a multiplex reverse transcription polymerase chain reaction (rt-pcr) was performed for rotavirus g and p genotypes. this current study was carried out between 2006 and 2009 during which time 1,607 (23%) of 7,058 fecal specim ... | 2011 | 21860255 |
| inhibitory effects of bile acids and synthetic farnesoid x receptor agonists on rotavirus replication. | rotaviruses (group a rotaviruses) are the most important cause of severe gastroenteritis in infants and children worldwide. currently, an antiviral drug is not available and information on therapeutic targets for antiviral development is limited for rotavirus infection. previously, it was shown that lipid homeostasis is important in rotavirus replication. since farnesoid x receptor (fxr) and its natural ligands bile acids (such as chenodeoxycholic acid [cdca]) play major roles in cholesterol and ... | 2011 | 21957312 |
| structural organisation of the rotavirus nonstructural protein nsp5. | rotavirus is one of the leading agents of gastroenteritis worldwide. during infection, viral factories (viroplasms) are formed. the rotavirus nonstructural proteins nsp5 and nsp2 are the major building blocks of viroplasms; however, nsp5 function and organisation remain elusive. in this report, we present a structural characterisation of nsp5. multi-angle laser light scattering, sedimentation velocity and equilibrium sedimentation experiments demonstrate that recombinant full-length nsp5 forms a ... | 2011 | 21864538 |
| group a rotavirus detection on environmental surfaces in a hospital intensive care unit. | background: environmental surfaces can play a role in the spread of pathogens, such as enteric viruses, within a hospital. this study assessed the level of contamination of group a rotavirus (rv-a) on environmental surfaces samples from an adult intensive care unit in a hospital in rio de janeiro, brazil. methods: a total of 504 environmental surface samples were obtained from multiple sites in the intensive care unit, including flushing buttons, telephones, and alcohol gel supports. nested and ... | 2011 | 22018841 |
| Molecular Techniques in Ecohealth Research Toolkit: Facilitating Estimation of Aggregate Gastroenteritis Burden in an Irrigated Periurban Landscape. | Assessment of microbial hazards associated with certain environmental matrices, livelihood strategies, and food handling practices are constrained by time-consuming conventional microbiological techniques that lead to health risk assessments of narrow geographic or time scope, often targeting very few pathogens. Health risk assessment based on one or few indicator organisms underestimates true disease burden due a number of coexisting causative pathogens. Here, we employed molecular techniques i ... | 2011 | 22146856 |
| monitoring the circulation of rotavirus among children after the introduction of the rotarixôäó vaccine in goi+ónia, brazil. | the epidemiological features of rotavirus a (rva) infection differ between children from developing and developed countries which could result in differences in vaccine efficacy around the world. to evaluate the impact of rotarixôäó on rva prevalence, we monitored rva genotypes circulating in goi+ónia by monitoring virus in faecal samples from children that had or had not been previously vaccinated. from february-november of 2008, 220 faecal samples were collected from children in seven day-care ... | 2011 | 21739040 |
| VP8* antigen produced in tobacco transplastomic plants confers protection against bovine rotavirus infection in a suckling mouse model. | Group A rotavirus is a major leading cause of diarrhea in mammalian species worldwide. In Argentina, bovine rotavirus (BRV) is the main cause of neonatal diarrhea in calves. VP4, one of the outermost capsid proteins, is involved in various virus functions. Rotavirus infectivity requires proteolytic cleavage of VP4, giving an N-terminal non-glycosilated sialic acid-recognizing domain (VP8*), and a C-terminal fragment (VP5*) that remains associated with the virion. VP8* subunit is the major determ ... | 2011 | 21893114 |
| complete molecular genome analyses of equine rotavirus a strains from different continents reveal several new genotypes and a largely conserved genotype constellation. | in this study, the complete genome sequence of seven equine group a rotavirus (rva) strains (rva/horse-tc/gbr/l338/1991/g13p[18], rva/horse-wt/irl/03v04954/2003/g3p[12] and rva/horse-wt/irl/04v2024/2004/g14p[12] from europe; rva/horse-wt/arg/e30/1993/g3p[12], rva/horse-wt/arg/e403/2006/g14p[12], and rva/horse-wt/arg/e4040/2008/g14p[12] from argentina, and rva/horse-wt/zaf/eqrv-sa1/2006/g14p[12] from south africa) were determined. multiple new genotypes were identified and genotype numbers were a ... | 2011 | 22190012 |
| prevalence of human rotavirus genotypes in wuhan, china, during 2008-2011: changing trend of predominant genotypes and emergence of strains with the p[8]b subtype of the vp4 gene. | hospital-based surveillance of rotavirus genotypes was conducted in wuhan, china, between march 2008 and may 2011. the detection rates of group a rotavirus were 24.6% (458/1859) and 12.1% (96/795) in children and adults, respectively, with diarrhea. among the 554 positive specimens, the most frequent genotype was g3p[8] (57.9%), followed by g1p[8] (29.4%). compared with previous studies in wuhan (2000-2008), the relative frequency of g3p[8] has been decreasing year by year, while the predominant ... | 2011 | 21986895 |
| prevalence and molecular genotyping of group a rotaviruses in iranian children. | rotavirus is the leading cause of acute gastroenteritis in worldwide young children. effective vaccines to prevent rotavirus infection are currently available, although their clinical use is still limited, and rotavirus still causes many episodes of infantile gastroenteritis, mainly during the winter season. the aim of this study was to evaluate the prevalence of rotavirus infection in children aged <5-years-old who were hospitalised for gastroenteritis. one hundred and sixty-three stool samples ... | 2012 | 23729998 |
| first report of human rotavirus g8p[4] gastroenteritis in india: evidence of ruminants-to-human zoonotic transmission. | group a rotaviruses are the major cause of childhood gastroenteritis worldwide. due to close proximity of human and cattle in rural areas of developing countries like india, interspecies transmission or zoonotic transmission is a major source of rapid generation of reassortants and genetic or antigenic variants. previously, many human group a rotaviruses were found with porcine or bovine characteristics from eastern and north-eastern india. in this study, four unusual human g8p[4] strains were i ... | 2012 | 23280762 |
| [experience of application of multiplex qpcr for differential diagnostics of intestinal viral infections]. | evaluate the effectiveness of multiplex reverse transcription (rt) and polymerase chain reaction with fluorescence detection in real time mode (qpcr) methods for differential detection of 11 groups of intestine viruses (adenoviruses, enteroviruses, polioviruses, hepatitis a and e viruses, group a and c rotaviruses, orthoreoviruses, noroviruses, sapoviruses and astroviruses) in various biological samples. | 2012 | 23297630 |
| genotyping of gastroenteric viruses in hospitalised children: first report of norovirus gii.21 in brazil. | this retrospective study (april-september 2003) was designed to investigate the roles of the main viruses responsible for cases of acute infantile gastroenteritis in hospitalised children up to two years of age. the viruses were identified in 64.7% (88/136) of the cases and the detection rates of rotavirus a (rva), norovirus (nov) and astrovirus were 41.9% (57/136), 30.3% (24/79) and 12.7% (7/55), respectively. rva and nov were detected in 20 of the 24 reported nosocomial infection cases. this s ... | 2012 | 23295760 |
| evaluation of a multiplex real time reverse transcription pcr assay for the detection and quantitation of the most common human rotavirus genotypes. | group a rotaviruses (rvs) are important pathogens that cause acute, dehydrating gastroenteritis in infants and young children. in this study, a multiplex real-time polymerase chain reaction protocol using primers and taqman(®) probes specific for viral vp4 and vp7 genes was evaluated. this assay offers simultaneous genotyping and quantification of the most common rv genotypes g1p[8], g2p[4], g3p[8], g4p[8], and g9p[8]. it was compared to the molecular typing results provided by conventional pcr. ... | 2012 | 22245180 |
| continuous genotyping for human rotavirus group a. | no abstract available. | 2012 | 23770884 |
| [the genetic stability of recombinant adenovirus expressing human rotavirus vp6 gene which used ad41 as vector]. | to investigate the genetic stability of non-replicating recombinant adenovirus which used ad41 as vector and could express vp6 gene of group a rotavirus during continous passage, in order to develop the vaccine of rotavirus. | 2012 | 23627019 |
| annual changes in predominant genotypes of rotavirus a detected in the feces of pigs in various developmental stages raised on a conventional farm. | the goal of the present study was to improve understanding of the ecology of porcine rotavirus a (rva) infection in pigs raised on a conventional farrow-to-finish farm. we collected 145 fecal samples over a 3-year period from suckling pigs and their dams, and pigs at 30, 60, 90, 120, and 150 days of age. reverse transcriptase-polymerase chain reaction analysis revealed that 29 samples (20%) were positive for the viral vp7 gene. the detection rate of vp7 sequences was highest in 30-day-old pigs ( ... | 2012 | 23313539 |
| a wide variety of diarrhea viruses circulating in pediatric patients in thailand. | viral gastroenteritis has been recognized as one of the most common illnesses that affects infants and young children all over the world. a wide variety of viruses associated with the disease are continually being reported. to investigate the epidemiological situation of diarrhea virus infection in chiang mai, thailand, surveillance was conducted during january to december 2007. | 2012 | 22372354 |
| human bocavirus 1 and 3 infection in children with acute gastroenteritis in brazil. | to determine the positivity rate of human bocavirus (hbov) 1 and 3 among children who presented with acute gastroenteritis symptoms during the period of 1994-2004 in the central-west region of brazil, 762 faecal samples were tested using polymerase chain reaction (pcr) for the detection of hbov dna. primers for a segment of the non-structural viral protein 1 (ns1) gene of hbov-1 and hbov-3 were used. twelve hbov-positive samples were further characterised via genomic sequencing and phylogenetic ... | 2012 | 22990971 |
| development and performance evaluation of calf diarrhea pathogen nucleic acid purification and detection workflow. | calf diarrhea (scours) is a primary cause of illness and death in young calves. significant economic losses associated with this disease include morbidity, mortality, and direct cost of treatment. multiple pathogens are responsible for infectious diarrhea, including, but not limited to, bovine coronavirus (bcv), bovine rotavirus a (brv), and cryptosporidium spp. identification and isolation of carrier calves are essential for disease management. texas veterinary medical diagnostic laboratory cur ... | 2012 | 22914823 |
| development and application of one-step multiplex reverse transcription pcr for simultaneous detection of five diarrheal viruses in adult cattle. | a one-step multiplex reverse transcription (rt)-pcr method was developed for the simultaneous detection of five viruses causing diarrhea in adult cattle: bovine group a rotavirus (gar), bovine group b rotavirus (gbr), bovine group c rotavirus (gcr), bovine coronavirus (bcv), and bovine torovirus (btov). the detection limit of the one-step multiplex rt-pcr for gar, gcr, bcv, and btov was 10(2), 10(0), 10(1), and 10(2) tcid(50)/ml, respectively, and that for gbr was 10(6) copies/ml. the one-step m ... | 2012 | 22407445 |
| evaluation of a commercial rapid test kit for detecting bovine enteric pathogens in feces. | recently a commercial antigen-capture enzyme-linked immunosorbent assay kit in the form of a dipstick (bovine enterichek, biovet inc.) was made available to bovine practitioners and producers for the rapid detection of betacoronavirus 1 (bcv-1), rotavirus a (rv-a), escherichia coli k99(+), and cryptosporidium parvum in feces from diarrheic calves. the diagnostic performance of bovine enterichek was evaluated in comparison with a multiplex real-time polymerase chain reaction assay (mrtpcr). one h ... | 2012 | 22529125 |
| molecular characterization of the nsp4 gene of human group a rotavirus strains circulating in tunisia from 2006 to 2008. | non-structural protein 4 (nsp4), encoded by group a rotavirus (rva) genome segment 10, is a multifunctional protein and the first recognized virus-encoded enterotoxin. recently, a new classification system for rvas was proposed and a total of 14 nsp4 genotypes (e1-e14) are currently described. the most common nsp4 genotypes in humans are wa-like e1 and ds-1-like e2. this report represents the first investigation on the genetic diversity of rva nsp4 genes in tunisia from 2006 to 2008. in the pres ... | 2012 | 22406141 |
| rotavirus a-specific single-domain antibodies produced in baculovirus-infected insect larvae are protective in vivo. | single-domain antibodies (sdabs), also known as nanobodies or vhhs, are characterized by high stability and solubility, thus maintaining the affinity and therapeutic value provided by conventional antibodies. given these properties, vhhs offer a novel alternative to classical antibody approaches. to date, vhhs have been produced mainly in e. coli, yeast, plants and mammalian cells. to apply the single-domain antibodies as a preventive or therapeutic strategy to control rotavirus infections in de ... | 2012 | 22953695 |
| detection of enteric viruses in recreational waters of an urban lagoon in the city of rio de janeiro, brazil. | the aim of this study was to determine the occurrences of the group a rotavirus (rva), norovirus (nov) and human adenovirus (hadv) in the surface waters of an urban lagoon (rodrigo de freitas lagoon) in the city of rio de janeiro, brazil. during one year of surveillance, water samples were obtained from the lagoon and other interconnected ecosystems (river and beach). the samples were concentrated using an adsorption-elution method with a negatively charged membrane and tested by qualitative and ... | 2012 | 22990969 |
| simultaneous investigation of influenza and enteric viruses in the stools of adult patients consulting in general practice for acute diarrhea. | gastrointestinal symptoms are not an uncommon manifestation of an influenza virus infection. in the present study, we aimed to investigate the presence of influenza viruses in the stools of adult patients consulting their general practitioner for uncomplicated acute diarrhea (ad) and the proportion of concurrent infections by enteric and influenza viruses. | 2012 | 22709374 |
| simultaneous detection of seven enteric viruses associated with acute gastroenteritis by a multiplexed luminex-based assay. | rapid and broad diagnostic methods are needed for the identification of viral agents of gastroenteritis. in this study, we used luminex xmap technology to develop a multiplexed assay for the simultaneous identification of major enteric viral pathogens, including rotavirus a (rva), noroviruses (novs) (including genogroups gi and gii), sapoviruses (sav), human astrovirus (hastv), enteric adenoviruses (eads), and human bocavirus 2 (hbov2). the analytical sensitivity allowed detection of 10(3) (eads ... | 2012 | 22518865 |
| hygienic monitoring of mongolian gerbils: which mouse viruses should be included? | the mongolian gerbil (meriones unguiculatus) serves as an animal model for a wide range of diseases. a practical limitation in its use is the definition of the hygienic status, as not much is known about viruses that potentially infect gerbils and might be transmitted to other rodents. as successful re-derivation was recently described for gerbils, we now aimed at investigating which mouse viruses induce seroconversion in gerbils and might be transmitted to mice. gerbils were inoculated with vir ... | 2012 | 22334874 |
| diversity in the enteric viruses detected in outbreaks of gastroenteritis from mumbai, western india. | faecal specimens collected from two outbreaks of acute gastroenteritis that occurred in southern mumbai, india in march and october, 2006 were tested for seven different enteric viruses. among the 218 specimens tested, 95 (43.6%) were positive, 73 (76.8%) for a single virus and 22 (23.2%) for multiple viruses. single viral infections in both, march and october showed predominance of enterovirus (ev, 33.3% and 40%) and rotavirus a (rva, 33.3% and 25%). the other viruses detected in these months w ... | 2012 | 22690171 |
| detection of rare reassortant g5p[6] rotavirus, bulgaria. | during the ongoing rotavirus strain surveillance program conducted in bulgaria, an unusual human rotavirus a (rva) strain, rva/human/bg/bg620/2008/g5p[6], was identified among 2200 genotyped bulgarian rvas. this strain showed the following genomic configuration: g5-p[6]-i1-r1-c1-m1-a8-n1-t1-e1-h1. phylogenetic analysis of the genes encoding the neutralization proteins and backbone genes identified a probable mixture of rva genes of human and porcine origin. the vp1, vp6 and nsp2 genes were more ... | 2012 | 22850117 |
| molecular characterization of a rare g9p[23] porcine rotavirus isolate from china. | the fifth most important g genotype, g9 rotavirus, is recognized as an emerging genotype that is spreading around the world. sequence analysis was completed of a rare group a rotavirus, strain g9p[23], that was designated rotavirus a pig/china/nmtl/2008/g9p[23] and abbreviated as nmtl. it was isolated from a piglet with diarrhea in china. nucleotide sequence analysis revealed that the vp7 gene clustered within the g9 lineage vid. the vp4 gene clustered within the rare p[23] genotype. nmtl is the ... | 2012 | 22729562 |
| antibody response in vaccinated pregnant mares to recent g3bp[12] and g14p[12] equine rotaviruses. | both the g3p[12] and the g14p[12] type of equine group a rotavirus (rva) have recently become predominant in many countries, including japan. g3 types are classified further into g3a and g3b. the g3a viruses have been circulating in europe, australia, and argentina, and the g3b viruses have been circulating in japan. however, only an inactivated vaccine containing a single g3bp[12] strain is commercially available in japan. to assess the efficacy of the current vaccine against recently circulati ... | 2012 | 23130609 |
| burden and typing of rotavirus group a in children with acute gastroenteritis in shiraz, southern iran. | human rotavirus is a significant cause of severe gastroenteritis in infants and young children worldwide. in recent years, rotavirus genotyping by rt-pcr has provided valuable information about the diversity of rotaviruses circulating worldwide. the purpose of the present study is to monitor the prevalence of the different g types of rotaviruses circulating in shiraz, southern iran and detect any uncommon or novel types. | 2012 | 23115715 |
| rotavirus genotyping in gastroenteritis cases of an infantile population from western brazilian amazonia. | during the period from 2000 to 2002, 79 rotavirus-positive stool samples were collected from children presenting diarrhea in the western brazilian amazon. | 2012 | 22930050 |
| rotavirus genotypes in malaysia and universal rotavirus vaccination. | group a rotavirus (rv-a) genotypes isolated in malaysia was studied to estimate the effectiveness of a universal rv-a vaccination in malaysia. a simple mathematical model was used, with input from a two-year, two-center, prospective study on hospitalization of rv-a gastroenteritis (rvge) in young children, published data on rv-a hospitalizations and genotypes, mortality on childhood ge and published genotype-specific efficacy data on two rv-a vaccines. assuming a 95% vaccine coverage, the overal ... | 2012 | 23022710 |
| frequency of group a rotavirus with mixed g and p genotypes in bovines: predominance of g3 genotype and its emergence in combination with g8/g10 types. | the present study describes the genotypic distribution of rotaviruses (rvs) in an indian bovine population with unexpectedly higher proportions of g3 alone or in combination of g8/g10. pcr-genotyping confirmed that 39.4% (13/33) of the prevalent rvs were the g3 type while 60.6% (20/33) were dual g3g10 or g3g8 types. p typing revealed that 93.9% (31/33) of the samples were p[11] while 6.1% (2/33) possessed a dual p[1]p[11] type. sequence analysis of the vp7 gene from g3 strains viz. b-46, 0970, a ... | 2012 | 23006956 |
| the first identification of rare human group a rotavirus strain g3p[10] with severe infantile diarrhea in eastern india. | during an ongoing surveillance for diarrheal pathogens, an unusual human group a rotavirus strain g3p[10] (rva/human-wt/ind/mcs60/2011/g3p[10]) was detected in a stool sample of a 14 months old girl child with acute diarrhea in kolkata, eastern india. the vp7 nucleotide sequence of this strain revealed a close phylogenetic relationship to the prototype g3 strain au-1 and australian feline strain cat2, whereas, the vp4 gene segment was closely related to the g8p[10] rotavirus 69m from indonesia. ... | 2012 | 22981998 |
| phylogenetic analysis of the rotavirus genotypes originated from children < 5 years of age in 16 cities in south korea, between 2000 and 2004. | the purpose of this study was to examine the diversity of the g and p types of human rotavirus strains isolated in south korea during 2000 to 2004. | 2012 | 24159485 |
| predominance of rotavirus genotype g6p[11] in diarrhoeic lambs. | out of 500 faecal samples from lambs with diarrhoea in jammu and kashmir, india, 66 (13.2%) were positive for group a rotavirus (garv) by the latex agglutination test (lat). electropherotyping by rna-polyacrylamide gel electrophoresis revealed the typical garv 4-2-3-2 migration pattern in 49/66 (74.2%) samples. fifty-two samples (10.4%) were positive by reverse transcription-pcr. g6 was the predominant g genotype (25/52; 48.07%), followed by g10 (19/52; 36.54%) whereas, the predominant p genotyp ... | 2012 | 22230710 |
| rotavirus in diarrheal children in rural burkina faso: high prevalence of genotype g6p[6]. | group a rotavirus (rva) is the most common cause of severe gastroenteritis in young children globally, and responsible for a significant number of deaths in african countries. while vaccines are available, trials have shown a lesser efficacy in africa. one of the reasons could be the prevalence and/or emergence of unusual or novel rva strains, as many strains detected in african countries remain uncharacterized. in this study, we characterized rva positive specimens from two remote rural areas i ... | 2012 | 22964045 |
| selection and evolutionary analysis in the nonstructural protein nsp2 of rotavirus a. | rotavirus a is the leading cause of acute gastroenteritis in infants and young children worldwide. the nonstructural protein 2 (nsp2) plays essential roles in the replication cycle of rotavirus and may play a role in protective immunity against rotavirus disease. using a bayesian approach, we measured the mutation rate of genotype n1 nsp2 gene sequences. the n1 genotype is the main nsp2 genotype associated with rotavirus strains causing severe disease, and was found to have a high mutation rate ... | 2012 | 22610044 |
| whole-genomic analysis of a human g1p[9] rotavirus strain reveals intergenogroup-reassortment events. | group a rotavirus (rva) strain k8 (rva/human-tc/jpn/k8/1977/g1p[9]) was found to have wa-like vp7 and nsp1 genes and au-1-like vp4 and nsp5 genes. to determine the exact origin and overall genetic makeup of this unusual rva strain, the remaining genes (vp1-vp3, vp6 and nsp2-nsp4) of k8 were analysed in this study. strain k8 exhibited a g1-p[9]-i1-r3-c3-m3-a1-n1-t3-e3-h3 genotype constellation, not reported previously. the vp6 and nsp2 genes of strain k8 were related closely to those of common hu ... | 2012 | 22592265 |
| the unpredictable diversity of co-circulating rotavirus types in europe and the possible impact of universal mass vaccination programmes on rotavirus genotype incidence. | this article reviews the incidence of group a rotavirus (rv) types isolated from children with acute gastroenteritis (age) in european countries during the last 5-10 years, with the aim of establishing an overview of rv diversity before the introduction of universal mass vaccination (umv) programmes against rv disease in most european countries. consistent with findings from previous surveys, a high degree of diversity of co-circulating rv types exists in different locations of europe, and over ... | 2012 | 22579864 |
| comparison of two eukaryotic systems for the expression of vp6 protein of rotavirus specie a: transient gene expression in hek293-t cells and insect cell-baculovirus system. | the vp6 protein of rotavirus a (rva) is a target antigen used for diagnostic assays and also for the development of new rva vaccines. we have compared the expression of vp6 protein in human embryonic kidney (hek293-t) cells with results obtained using a well-established insect cell-baculovirus system. the recombinant vp6 (rvp6) expressed in hek293-t cells did not present degradation and also retained the ability to form trimers. in the insect cell-baculovirus system, rvp6 was expressed at higher ... | 2012 | 22576283 |
| full genome characterization of a porcine-like human g9p[6] rotavirus strain isolated from an infant in belgium. | interspecies transmissions of group a rotavirus (rva) strains among animals and humans are thought to take place frequently. during a rva surveillance study in belgium we isolated an unusual g9p[6] rva strain, rva/human-wt/bel/be2001/2009/g9p[6], from a 1month old boy, which did not cluster with other g9 or p[6] strains isolated in belgium. in this study we sequenced and characterized the complete genome of this unusual g9p[6] strain be2001. phylogenetic analyses of all 11 genes revealed a uniqu ... | 2012 | 22430049 |
| genetic variation in the vp7 gene of rotavirus g1p[8] strains isolated in vietnam, 1998-2009. | group a rotavirus genotype g1p[8] is the most common strain affecting humans around the world over the past few decades. in this study, we examined genetic variation in the vp7 gene of rotavirus g1p[8] strains, detected in children of four major cities of vietnam during three different rotavirus seasons: 1998-1999, 2007-2008 and 2008-2009 in order to assess the evolution of the virus over 11 years. fecal samples (n=73) from children hospitalized for gastroenteritis caused by g1p[8] rotavirus wer ... | 2012 | 22387966 |
| group a rotavirus genotypes circulating prior to implementation of a national immunization program in estonia. | group a rotaviruses (rva) are a major cause of acute gastroenteritis in children ≤ 5 y worldwide which could be prevented with two recently introduced vaccines - monovalent rotarix (live-attenuated g1p[8] strain) and pentavalent rotateq (human-bovine reassortant containing serotypes g1, g2, g3, g4 and p[8]). prior to implementation of vaccines into national immunization program we aimed to describe rva genotype distribution in hospitalized children aged < 5 y in estonia during 2007-2008. a total ... | 2012 | 22370511 |
| a large outbreak of enteritis in goat flocks in marmara, turkey, by g8p[1] group a rotaviruses. | group a rotaviruses are regarded as major enteric pathogens of large ruminants, while their impact on the health of small ruminants is not well documented. we report the detection of group a rotavirus from a large outbreak of enteritis that occurred in two goat flocks in the town of kırklareli, marmara region, turkey, in 2007. the disease was observed in young kids, with high morbidity and mortality rates, but not in adult animals. rotavirus antigen was detected in the stools of the examined ani ... | 2012 | 22367501 |
| detection of avian group d rotavirus using the polymerase chain reaction for the vp6 gene. | group d rotaviruses (rvs-d) have been documented in birds and, while they may be common in these animals, few molecular studies are available for this specific group. in this study, specific primers for the gene that encodes for the rvs-d vp6 protein were designed and used in a reverse transcription polymerase chain reaction (rt-pcr). thirty pools of samples were tested by polyacrylamide gel electrophoresis (page) yielding a 30% (9/30) positivity. these pools were subjected subsequently to rt-pc ... | 2012 | 22820073 |
| full genome analysis of group b rotaviruses from western india: genetic relatedness and evolution. | to date, full-genome sequences of only seven human group b rotavirus (rvbs) strains have been described. such data on more rvbs are necessary to establish the evolutionary relationship and ecological features of rvbs from different geographical regions. the present study was aimed at determining the full-length sequences of all 11 genes of 13 human rvb strains detected during 1995-2010 in sporadic and outbreak cases of acute gastroenteritis from four different cities of western india. this study ... | 2012 | 22815276 |
| prevalence and factors associated with group a rotavirus infection among children with acute diarrhea in mwanza, tanzania. | rotavirus infections frequently cause acute gastroenteritis in humans and are the most important cause of severe dehydrating diarrhea in young children in both developed and developing countries. | 2012 | 22706194 |
| genotype constellation and evolution of group a rotaviruses infecting humans. | numerous rotavirus group a (rva) strains with distinct g-genotype and p-genotype combinations have been described infecting humans worldwide. however, the increasing amount of complete rva genome data which have become available, suggest that only rva strains with 2 discrete genotype constellations have been successful in sustaining infection of humans worldwide over longer periods of time. those genotype constellations have been designated i1-r1-c1-m1-a1-n1-t1-e1-h1 and i2-r2-c2-m2-a2-n2-t2-e2- ... | 2012 | 22683209 |
| epidemiology of rotavirus diarrhea in children under 5 years in northern cameroon. | rotavirus still remains the major cause of diarrhea in children below 5 years. no data on rotavirus epidemiology is available in the northern regions of cameroon. we aimed to determine the prevalence of group a rotavirus (rva) in children below 5 years with diarrhea in two regions of northern cameroon (north west and far north regions) so as to improve our knowledge on the burden of rotavirus disease for imminent introduction of a rotavirus vaccine. | 2012 | 22655107 |
| real-time pcr detection of enteric viruses in source water and treated drinking water in wuhan, china. | a total of 48 water samples were collected from six water treatment plants in wuhan and analyzed by real-time pcr assay for viral identification of enterovirus (ev), rotavirus group a (rva), human adenovirus (hadv) as well as human adenovirus subgroup f (hadvf) during the period from december 2010 to october 2011. hadv, hadvf, and rva were all positively detected in the samples of source water and treated drinking water. ev could be found in 46 % (11/24) of all the source water samples, but only ... | 2012 | 22645016 |
| repeated circulation over 6 years of intergenogroup mono-reassortant g2p[4] rotavirus strains with genotype n1 of the nsp2 gene. | human rotavirus a, a major cause of acute diarrhoea in infants and young children worldwide, contains the genome of 11 segments of double-stranded rna, which is grouped into three major genotype constellations: the wa genogroup (g1-p[8]-i1-r1-c1-m1-a1-n1-t1-e1-h1), the ds-1 genogroup (g2-p[4]-i2-r2-c2-m2-a2-n2-t2-e2-h2), and the au-1 genogroup (g3-p[9]-i3-r3-c3-m3-a3-n3-t3-e3-h3). recently, a g2p[4] strain detected in kenya in 1982 was reported to be a nonstructural protein 2 (nsp2) gene mono-re ... | 2012 | 22575821 |