Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| the first complete genome sequence of a chicken group a rotavirus indicates independent evolution of mammalian and avian strains. | group a rotaviruses are a leading cause of gastroenteritis in humans and animals. transmission between mammalian species and humans has been demonstrated repeatedly. here, the first entire genome sequence (19,064 bp) of a chicken rotavirus, strain ch-02v0002g3, is presented. a low degree of nucleotide sequence identity with the mammalian group a rotaviruses is evident for all 11 genome segments, whereas a closer relationship to available rotavirus sequences from avian species has been determined ... | 2009 | 19246068 |
| detection of human viruses in rivers of a densly-populated area in germany using a virus adsorption elution method optimized for pcr analyses. | transmission of viruses via surface water is a major public health concern. to determine the viral concentration in rivers of a densely-populated area in germany, the virus adsorption elution (viradel) method was optimized for downstream pcr applications. using a high-salt alkaline phosphate buffer for elution, the median recovery efficiency from spiked 1l water samples ranged from 21.3% to 100% for jc polyomavirus, human adenovirus type 5, echovirus 11, and norovirus genogroup i. analyses of 41 ... | 2009 | 19361832 |
| seasonal shifts of group a rotavirus strains as a possible mechanism of persistence in the human population. | this article demonstrates how the seasonal predominance of a new rotavirus strain in asuncion, paraguay is correlated with a wide spectrum of age groups of children infected in that given season. therefore, this study provides new evidence to support the idea that seasonal shift of rotavirus strains is a possible mechanism used by the virus to evade herd immunity (acquired by the population due to previous infections) and, thus, ultimately persist in that population. | 2009 | 19152398 |
| unexpected substitution of dominant rotavirus g genotypes in french hospitalized children over five consecutive seasons. | the study was designed to evaluate the circulation of group a rotaviruses in french hospitalized children, and to detect unusual strains. this prospective study was conducted from 2001 to 2006 in children consulting for acute diarrhea at the pediatric emergency department in three french university hospitals. the rotaviruses were detected by rapid test and genotyped by rt-pcr on the basis of their outer capsid proteins vp4 (p-type) and vp7 (g-type). the stools from 757 children were analyzed. g1 ... | 2009 | 18855026 |
| relationship between electropherotypes and vp7/vp4 genotypes of group a rotaviruses detected between 2000 and 2007 in tunisian children. | background: rotaviruses are the most frequent agents associated with diarrhoea in children worldwide. analysis of mobility of the 11 segments of genomic rna by polyacrylamide gel electrophoresis (page) yields a pattern which is characteristic for a particular rotavirus isolate. the group a rotaviruses can be further characterized by analysis of vp7 and vp4 genes specificities, responsible for rotavirus classification into g and p genotypes, respectively. the aim of the present study was to detec ... | 2009 | 19481882 |
| [norovirus infection in general hospitals for adults]. | to determine the role of noroviruses in the rise of hospital outbreaks of acute intestinal infections (aii), to study clinical and epidemiological aspects of hospital noroviral infection. | 2009 | 19514424 |
| evaluation of rapid assays for the detection of bovine coronavirus, rotavirus a and cryptosporidium parvum in faecal samples of calves. | rapid immunochromatographic assays for detecting infections with bovine coronavirus (bcv), rotavirus a and cryptosporidium parvum in calf faeces were evaluated using as gold standards a reverse transcriptase polymerase chain reaction (bcv and rotavirus) and a sedimentation-flotation technique (c. parvum). rapid tests for the detection of bcv and rotavirus showed a high specificity (96.4% and 95.3%, respectively), but a relatively low sensitivity (60.0% and 71.9%, respectively). sensitivity and s ... | 2009 | 18778958 |
| assignment of the group a rotavirus nsp4 gene into genotypes using a hemi-nested multiplex pcr assay: a rapid and reproducible assay for strain surveillance studies. | the rotavirus non-structural protein nsp4 has been implicated in a number of biological functions during the rotavirus cellular cycle and pathogenesis, and has been addressed as a target for vaccine development. the nsp4 gene has been classified into six genotypes (a-f). a semi-nested triplex pcr was developed for genotyping the major human nsp4 genotypes (a-c), which are common in human rotavirus strains but are also shared among most mammalian rotavirus strains. a total of 192 previously chara ... | 2009 | 19208878 |
| evidence of interspecies transmission and reassortment among avian group a rotaviruses. | avian rotaviruses are broadly distributed among birds, but only scarcely characterized on the molecular level. the vp4-, vp6-, vp7- and nsp5-encoding sequences of eight group a rotaviruses from chickens and turkeys determined here indicate a low degree of sequence similarity with mammalian rotaviruses. an nsp6-encoding region was missing in all chicken isolates except for isolate ch2. four novel genotypes (p[30], p[31], g22 and h8) were assigned by the rotavirus classification working group. gen ... | 2009 | 19249805 |
| first molecular detection of group a rotaviruses in drinking water sources in beijing, china. | the most prevalent group a rotavirus found in the diarrheic children was also determined in drinking water sources including raw water, treated water and tap water in beijing, and then the possible contamination contributions to tap water for human consumption were discussed in this study. a total of 26 raw water samples, 77 treated water samples and 143 tap water samples in beijing were collected for analysis of group a rotavirus from april 2006 to august 2007. according to the results, it was ... | 2009 | 19330275 |
| prospective study of etiologic agents of acute gastroenteritis outbreaks in child care centers. | to investigate the etiology of outbreaks of acute gastroenteritis (age) in children attending childcare centers (cccs) in north carolina between october 2005 and march 2007. | 2009 | 18783794 |
| diagnosing rotavirus a associated iid: using elisa to identify a cut-off for real time rt-pcr. | the use of rt-pcr for diagnosis of group a rotaviruses is increasing, but up to 14% of healthy individuals may be positive by rt-pcr. if rt-pcr is not well correlated with disease, rotavirus a may not always be the cause of illness in rt-pcr positive patients with infectious intestinal disease (iid). | 2009 | 19179107 |
| clinical profile and prevalence of rotavirus infection in children presented with acute diarrhea at tertiary care referral hospital at northern part of india. | a prospective analysis of 90 clinically diagnosed cases with acute diarrhea over a period of one year was carried out to determine the prevalence of rotavirus infection in children between 2 months to 2 years of age. enzyme linked immunosorbent assay (elisa) and polyacrylamide gel electrophoresis (page) were used for detection of rotavirus from stool sample. fourteen (15.6%) of them were found to be positive for group a rotavirus, 9 (23%) cases were between 6 months to 1 year of age. rotavirus e ... | 2009 | 22010485 |
| increased sensitivity for various rotavirus genotypes in stool specimens by amending three mismatched nucleotides in the forward primer of a real-time rt-pcr assay. | the real-time taqman rt-pcr assay (pang et al., 2004) did not detect 14 clinical samples with rotavirus g2 genotype. three to five nucleotides (nt) were found to be mismatched between the published forward primer when compared to g2p[4], g2p[8], g3p[4], g9p[4], g8 and g12 sequences. an additional forward primer was designed and included in a modified assay to test the 14 clinical samples and 12 samples with known rotavirus g and p genotypes. the modified assay has improved significantly the sens ... | 2010 | 21185331 |
| novel group a rotavirus g8 p[1] as primary cause of an ovine diarrheic syndrome outbreak in weaned lambs. | rotavirus is a worldwide major cause of diarrhea outbreaks in neonatal ruminants. an outbreak of ovine diarrheic syndrome (ods) in 50-75 days-old lambs (weaned lambs) is described. fecal immunochromatography and intestinal immunohistochemistry for rotavirus group a were performed. in addition, semi-nested multiplex rt-pcr for g and p rotavirus genotyping in combination with sequencing were performed, to support the diagnosis and identify the viral strain. a novel ovine rotavirus group a g8 p[1] ... | 2010 | 21216110 |
| rotavirus a genotype g1p[8]: a novel method to distinguish wild-type strains from the rotarix vaccine strain. | rotaviruses are important enteric pathogens for humans and animals. group a rotaviruses (rv-a) are the most common agents of severe gastroenteritis in infants and young children and vaccination is the most effective method to reduce rv-a-associated diseases. g1p[8], the most prevalent rv-a genotype worldwide, is included in the rv-a vaccine rotarix®. the discrimination between wild-type g1p[8] and vaccine g1p[8] strains is an important topic in the study of rv-a epidemiology to manage outbreaks ... | 2010 | 21225209 |
| genetic characterization of norovirus gii.4 2006b variants from jeju island, south korea. | ninety-seven fecal specimens collected from children with acute gastroenteritis between 2007 and 2008 that were found to be negative for group a rotavirus in prescreening by elisa with vp6-specific antibody were re-screened for viruses by reverse transcription (rt)-pcr. forty (41.2%) samples were found to be positive for virus by rt-pcr; of these, norovirus (32.5%, n = 13) and rotavirus (32.5%, n = 13) were the most common, followed by astrovirus (5.0%, n = 2) and enterovirus (2.5%, n = 1). co-i ... | 2010 | 20419823 |
| enteric calicivirus and rotavirus infections in domestic pigs. | we report the prevalence of rotavirus and calicivirus infections, along with their respective association with diarrhoea in the porcine population of the region of northern spain. a total of 221 samples were collected at random from different farms in the region and from the main slaughterhouse facility in the city of zaragoza. faecal samples were scored as diarrhoeic or normal and grouped into five groups to match general farm management and age criteria: group i (suckling 0-4 weeks), group ii ... | 2010 | 19781118 |
| molecular characterization of the vp1, vp2, vp4, vp6, nsp1 and nsp2 genes of bovine group b rotaviruses: identification of a novel vp4 genotype. | studies on bovine group b rotaviruses (gbrs) are limited. to date, only the vp6 gene of a single bovine gbr strain and the vp7 and nsp5 genes of a few bovine gbr strains have been sequenced and analyzed. in the present study, using a single-primer amplification method, we have determined the full-length nucleotide sequences of the vp1, vp2, vp4, vp6, nsp1 and nsp2 genes of three bovine gbr strains from eastern india. in all six of these genes, the bovine gbr strains shared high genetic relatedne ... | 2010 | 19936611 |
| distribution of g (vp7) and p (vp4) genotypes of group a bovine rotaviruses from turkish calves with diarrhea, 1997-2008. | group a rotaviruses are major enteric pathogens of calves. in order to investigate the genetic diversity of bovine rotaviruses (brvs), a collection of 53 brvs, detected from diarrheic calves from several turkish geographical areas, between 1997 and 2008 was analyzed by rt-pcr for specificities of the outer capsid proteins vp7 (g type) and vp4 (p type), for the first time. overall, g6 was the predominant g type, detected in 40/53 samples (75.4%), while p[11] was the predominant p type, detected i ... | 2010 | 19854003 |
| rotavirus-associated diarrhoea in foals in greece. | severe outbreaks of diarrhoeic syndrome occurred in young foals at the same stud farm during two consecutive breeding periods namely spring 2006 and 2007. rotavirus-like particles were detected by electron microscopy in the faeces of the affected foals and group a rotavirus infection was confirmed by reverse-transcription (rt)-pcr with selected sets of rotavirus-specific primers. sequence analysis of the genes encoding the outer capsid rotavirus proteins vp7 and vp4 enabled classification of the ... | 2010 | 20197218 |
| phylogenetic analysis of human p[8]g9 rotavirus strains circulating in brazil reveals the presence of a novel genetic variant. | group a rotavirus (rv-a) genotype p[8]g9 has emerged as one of the leading causes of gastroenteritis in children worldwide and currently is recognized as one of the five most common genotypes detected in humans. high intragenotype diversity in g9 rv-a has been observed, and nowadays, based on the genetic variability of the vp7 gene, six different phylogenetic lineages and eleven sublineages were described. | 2010 | 20133185 |
| zoonotic aspects of rotaviruses. | rotaviruses are important enteric pathogens of humans and animals. group a rotaviruses (garvs) account for up to 1 million children deaths each year, chiefly in developing countries and human vaccines are now available in many countries. rotavirus-associated enteritis is a major problem in livestock animals, notably in young calves and piglets. early in the epidemiological garv studies in humans, either sporadic cases or epidemics by atypical, animal-like garv strains were described. complete ge ... | 2010 | 19781872 |
| analysis of rotavirus non-structural protein nsp5 by mass spectrometry reveals a complex phosphorylation pattern. | genomic replication and partial assembly of rotavirus takes place in cytoplasmic viral structures called viroplasms. nsp5 is a viral phosphoprotein localized in viroplasms and its expression is imperative for viral cycle progress. during infection three isoforms of nsp5 can be observed by sds-page (26, 28 and 33-35kda) and previous reports suggested that they differ in their phosphorylation patterns. in this study we obtained nsp5 from infected cells and by mass spectrometry we were able to iden ... | 2010 | 20036292 |
| detection of rotavirus a in sewage samples using multiplex qpcr and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration. | group a rotaviruses (rv-a) are the most common agents of viral gastroenteritis in children worldwide. the goal of this study was to compare two different methods to concentrate rv-a from sewage samples and to improve the detection and quantification of rv-a using a multiplex quantitative pcr assay with an internal control. both rv-a and the internal control virus, bacteriophage pp7, were seeded into wastewater and then concentrated using either an ultrafiltration-based adsorption-elution protoco ... | 2010 | 20804786 |
| multiple-gene characterization of rotavirus strains: evidence of genetic linkage among the vp7-, vp4-, vp6-, and nsp4-encoding genes. | a total of 162 rotavirus strains detected between 1996 and 2006 among individuals with diarrhea in rio de janeiro, brazil, were analyzed by multiple-gene genotyping. characterization of strains was done by rt-pcr assay for amplification and typing of the vp7-, vp4-, vp6-, and nsp4-encoding genes. overall, 139 (85.8%) strains belonged to the common group a rotavirus combinations: 67 (41.4%) belonged to genotype g1-p[8]-i1-e1; 18 (11.1%) were g2-p[4]-i2-e2; 11 (6.8%) were g3-p[8]-i1-e1; 12 (7.4%) ... | 2010 | 20827779 |
| diagnosis of viral gastroenteritis by simultaneous detection of adenovirus group f, astrovirus, rotavirus group a, norovirus genogroups i and ii, and sapovirus in two internally controlled multiplex real-time pcr assays. | norovirus, rotavirus group a, astrovirus, sapovirus and adenovirus serotypes 40 and 41, are common causes of gastroenteritis. conventional diagnosis of these causative agents is based on antigen detection and electron microscopy. | 2010 | 20829103 |
| outbreak of acute gastroenteritis in young children with death due to rotavirus genotype g9 in rio branco, brazilian amazon region, 2005. | an epidemic of acute gastroenteritis occurred in rio branco city, acre state, in brazil's amazon region in 2005. an investigation was conducted to confirm the etiology and identify possible risk factors for death. | 2010 | 20843719 |
| genetic variation of g4p[6] rotaviruses: evidence for novel strains circulating between the hospital and community. | one hundred forty-six fecal specimens collected between 2007 and 2008 from infants with acute gastroenteritis were screened for rotavirus by elisa with vp6-specific antibody. one hundred twenty-three of the samples (84.2%) were confirmed to be positive for group a rotavirus (community-acquired, n = 90 [73.2%] and nosocomial, n = 33 [26.8%]), and were typed subsequently using rt-pcr and sequence analysis methods. determination of g- and p-type combinations showed that g4p[6] (78.9%) was the most ... | 2010 | 20166174 |
| multiplex nested rt-pcr for the detection of porcine enteric viruses. | porcine epidemic diarrhea virus (pedv), transmissible gastroenteritis virus (tgev), and porcine group a rotavirus (prv-a) are major viruses causing enteric diseases of piglets. a multiplex nested reverse transcription polymerase chain reaction (multiplex nested rt-pcr) was developed for the detection of these viruses in field samples from piglets with diarrhea. a mixture of (1) three external pairs of primers, yielding in the amplification step two different amplicons with sizes of 950 bp and 31 ... | 2010 | 20170679 |
| rotavirus nsp5 orchestrates recruitment of viroplasmic proteins. | rotavirus genome replication and the first steps of virus morphogenesis take place in cytoplasmic viral factories, called viroplasms, containing four structural (vp1, vp2, vp3 and vp6) and two non-structural (nsp2 and nsp5) proteins. nsp2 and nsp5 have been shown to be essential for viroplasm formation and, when co-expressed in uninfected cells, to form viroplasm-like structures (vls). in the present work, vls formation was shown upon co-expression of nsp5 with the core protein vp2 despite the a ... | 2010 | 20200190 |
| circulating human group a rotavirus genotypes in malaysia. | this study examined the temporal distribution of rotavirus genotypes in malaysia. rotaviruses from children with diarrhea admitted to hospitals in 1996 (n = 93) and 2007 (n = 12) in two different regions of peninsular (west) malaysia were analyzed for their g and p genotypes using a hemi-nested rt-pcr assay. in the 2007 samples, the dominant strain was g9p[8]. it was identified in 42% of the samples. different strains all possessing the g1 genotype were identified in the rest of the samples. in ... | 2010 | 20166178 |
| rotavirus antigenemia and genomia in children with rotavirus gastroenteritis. | we investigated group a rotavirus (garv) antigenemia and genomia in children with rotavirus gastroenteritis. a total of 16 patients (2-29 months old), who received a diagnosis of garv gastroenteritis using a commercial rapid test, were enrolled in this study. the sera from the patients were tested for the presence of garv antigen and the vp7 and nsp3 genes using an enzyme-linked immunosorbent assay (elisa) and reverse transcription-polymerase chain reaction, respectively. furthermore, when the v ... | 2010 | 20332567 |
| geographical differences in the proportion of human group a rotavirus strains within new zealand during one epidemic season. | the prospect of future rotavirus vaccine programs means it is important to understand rotavirus strain diversity within new zealand, especially if this was to influence vaccine effectiveness. the g-genotype of 359 group a rotavirus strains isolated from 416 stool samples collected from june 2005 to may 2006 (inclusive) from children less than 5 years of age in multiple centers throughout new zealand was determined. g1 was the dominant circulating strain (55.8%) followed by g4 (21.4%), g3 (3.4%), ... | 2010 | 20336723 |
| detection and genotyping of korean porcine rotaviruses. | porcine group a rotavirus (garv) is considered to be an important animal pathogen due to their economic impact in the swine industry and its potential to cause heterologous infections in humans. this study examined 475 fecal samples from 143 farms located in 6 provinces across south korea. rt-pcr and nested pcr utilizing primer pairs specific for the garv vp6 gene detected garv-positive reactions in 182 (38.3%) diarrheic fecal samples. a total of 98 porcine garv strains isolated from the garv-po ... | 2010 | 20359834 |
| noroviruses as cause of gastroenteritis in elderly patients. | background and aims. gastroenteritis is one of the infections that are particularly important to the elderly people. the knowledge of the main pathogens causing gastroenteritis in this group of patients, whose number will be dramatically increased in coming decades, is essential. the contribution of group a rotavirus, adenovirus type 41 and 42, norovirus and astrovirus as cause of gastroenteritis among patients of all ages, especially those with more than 65 years old over an extended time perio ... | 2010 | 20460955 |
| detection of uncommon rotavirus a strains p[8]g8 and p[4]g8 in the city of rio de janeiro, 2002. | group a rotaviruses (rv-a) are the major cause of gastroenteritis in infants and young children around the world. each year rv-a causes approximately 11 million episodes of severe diarrhea, with an estimated of 611,000 deaths. epidemiologic surveys have identified p[8]g1, p[4]g2, p[8]g3, p[8]g4, and p[8]g9 as the most common global genotypes associated with diarrhea in children up to 5-year old. surveillance studies and documentation of rv-a g and p genotypes is necessary for a comprehensive eva ... | 2010 | 20513095 |
| correlation between rotavirus a genotypes detected in hospitalized children and sewage samples in 2006, córdoba, argentina. | routine rotavirus a (rv-a) surveillance is based on clinical cases, so only symptomatic infections are reported. the objective of this study was to determine whether the rv-a genotypes and cold seasonal pattern described in patients with diarrhea is reflected by sewage surveillance, which could be representative of the rv-a genotypes circulating in the population. the genotype distribution of rv-a in effluent samples from a local sewage treatment plant was compared to those from local clinical c ... | 2010 | 20513096 |
| detection and characterisation of group a rotavirus in asymptomatic piglets in southern ireland. | porcine group a rotaviruses (garv) are causative agents of enteritis in piglets and are a large reservoir of genetic material for the diversification of human garvs. accumulation of information on the genetic heterogeneity of porcine viruses is pivotal for readily characterising unusual human strains. screening of 292 fecal samples, collected from 4-5- to 8-9-week-old asymptomatic pigs from four herds in ireland between 2005 and 2007 resulted in 19 (6.5%) samples testing positive by reverse-tran ... | 2010 | 20526785 |
| [rotavirus prevalence in infants and children in the public healthcare system of the state of pernambuco]. | rotaviruses are considered important etiological agents of acute gastroenteritis and a common cause of the hospitalization of children aged zero to four years-old. in brazil, the incidence of rotavirus gastroenteritis in children is 12 to 42% and the distribution of infection is related to seasonality, which apparently occurs in different periods and intensity according to each region. the study investigated group a rotavirus in fecal samples of suspected children attended by the public health s ... | 2010 | 21085867 |
| comparison of the rapid methods for screening of group a rotavirus in stool samples. | in this study, the sensitivity and specificity of three immunochromatography (ic) test kits for rapid detection of group a rotavirus were compared and evaluated with stool samples collected from children, who suffered from acute gastroenteritis during february to june, 2009 in japan. a total of 86 stool samples were tested and compared with a reference rt-pcr method. the sensitivity among ip-rota v, dipstick eiken rota and rota-adeno test kits were 97.2, 95.8 and 88.7%, while the specificity wer ... | 2010 | 21030457 |
| full genomic analysis of a simian sa11-like g3p[2] rotavirus strain isolated from an asymptomatic infant: identification of novel vp1, vp6 and nsp4 genotypes. | we report here the full genomic analysis of a simian sa11-like g3p[2] group a rotavirus (gar) strain, b10, isolated from an asymptomatic infant in kenya in 1987. by nucleotide sequence identities and phylogenetic analyses, the vp7-vp4-vp2-vp3-nsp1-nsp2-nsp3-nsp5 genes of strain b10 exhibited maximum genetic relatedness to those of the different isolates of simian strain sa11, and were assigned to the g3-p[2]-c5-m5-a5-n5-t5-h5 genotypes, respectively. on the other hand, the vp1, vp6 and nsp4 gene ... | 2010 | 21035567 |
| quantification and molecular characterization of enteric viruses detected in effluents from two hospital wastewater treatment plants. | hospital wastewater has been described as an important source of spreading pathogenic microorganisms in the environment. however, there are few studies reporting the presence and concentrations of gastroenteric viruses and hepatitis a viruses in these environmental matrices. the aim of this study was to assess the contamination by viruses responsible for acute gastroenteritis and hepatitis derived from hospital wastewater treatment plants (wwtps). rotavirus a (rv-a), human adenoviruses (hadv), n ... | 2010 | 21040941 |
| [detection of bovine rotavirus g10p[11] in a diary farm in daqing, china]. | group a rotavirus are the most frequently detected viral agent associated with the acute diarrhea in calves. in order to investigate the situation of rotavirus strains circulating in diary farms, a total of 117 fecal specimens were collected from diarrhea calves under 4 weeks-age on yinluo diary farm in daqing region in china from 2008 to 2009. ten specimens were detected to be positive by a rotavirus group a diagnostic kit, which confirmed that the rotavirus was important viral agent associated ... | 2010 | 21043143 |
| epidemiology and clinical features of gastroenteritis in hospitalised children: prospective survey during a 2-year period in a parisian hospital, france. | rotavirus is recognised as the most important agent of severe acute gastroenteritis (age) in young children. in a 2-year prospective survey, we investigated the epidemiology and clinical features of the viral and bacterial pathogens in children hospitalised for age. the study was performed in a parisian teaching hospital from november 2001 to may 2004. clinical data were prospectively collected to assess the gastroenteritis severity (20-point vesikari severity score, the need for intravenous reh ... | 2010 | 21128089 |
| genome sequence based molecular epidemiology of unusual us rotavirus a g9 strains isolated from omaha, usa between 1997 and 2000. | after discovery in the early 1980s, rotavirus a serotype g9 was detected infrequently for almost a decade. since the mid-1990s, however, serotype g9 has emerged to become a globally common strain linked to the introduction of a single, new genetic variant of g9 vp7 gene. studies have demonstrated that genetically divergent g9 strains co-circulated at low frequency with the emerging variants. examples include unique u.s. g9 strains om46/hu/usa/1998 and om67/hu/usa/1998, isolated in omaha during t ... | 2010 | 21130184 |
| [epidemiological surveillance of norovirus and rotavirus diarrhea among outpatient children in five metropolitan cities]. | to survey the clinical epidemiological features of norovirus and rotavirus diarrhea among children living in 5 cities. | 2010 | 21055297 |
| a hospital based study on inter- and intragenotypic diversity of human rotavirus a vp4 and vp7 gene segments, germany. | efforts to reduce the impact of group a rotaviruses on human morbidity and mortality rely on oral immunisation with live attenuated or recombinant vaccines. a major challenge in immunisation is the vast inter- and intragenotypic diversity accomplished by circulating rotaviruses. | 2010 | 21074491 |
| predominance of porcine p[23] genotype rotaviruses in piglets with diarrhea in northern thailand. | of 131 stool samples collected from piglets with diarrhea in northern thailand between july 2006 and august 2008, 14 (10.7%) were positive for group a rotavirus. sequence analysis showed that 13 strains (92.9%) belonged to the rare p[23] genotype combination with g9 or g3 genotypes. | 2010 | 21084504 |
| determination of g and p type diversity of group a rotaviruses in faecal samples of diarrhoeic calves in kashmir, india. | to determine the genetic diversity of group a rotaviruses in bovine calves in kashmir, india. | 2010 | 20875036 |
| reassortant group a rotavirus from straw-colored fruit bat (eidolon helvum). | bats are known reservoirs of viral zoonoses. we report genetic characterization of a bat rotavirus (bat/ke4852/07) detected in the feces of a straw-colored fruit bat (eidolon helvum). six bat rotavirus genes (viral protein [vp] 2, vp6, vp7, nonstructural protein [nsp] 2, nsp3, and nsp5) shared ancestry with other mammalian rotaviruses but were distantly related. the vp4 gene was nearly identical to that of human p[6] rotavirus strains, and the nsp4 gene was closely related to those of previously ... | 2010 | 21122212 |
| changing profile of the bovine rotavirus g6 population in the south of ireland from 2002 to 2009. | bovine group a rotavirus is one of the main causes of neonatal diarrhoea in calves. this study examined the different g and p genotypes circulating in the bovine population, from 2002-2009, in the south of ireland. rotavirus positive bovine faecal samples (n=332) were collected from the cork regional veterinary laboratory, between 2002 and 2009 and subjected to rna extraction, page analysis, and g and p genotyping. genotyping analysis identified g6, g10, p[5], and p[11] to be the predominant g a ... | 2010 | 20541335 |
| chemical and microbiological parameters as possible indicators for human enteric viruses in surface water. | there are still conflicting results on the suitability of chemical and microbiological parameters as indicators for the viral contamination of surface waters. in this study, conducted over 20 months, the abundance of human adenovirus, human polyomavirus, enterovirus, group a rotavirus and norovirus was determined in ruhr and rhine rivers, germany. additionally, prevalence of different possible indicators such as somatic coliphages, e. coli, intestinal enterococci, and total coliforms was also co ... | 2010 | 20627679 |
| detection and molecular characterization of enteric viruses in environmental samples in monastir, tunisia between january 2003 and april 2007. | a prospective study was performed to characterize the main human enteric viruses able to persist in sewage samples and in shellfish tissues, and to establish the correlation between environmental strains and viral infantile diarrhoea observed in the same area during the same period. | 2010 | 20553345 |
| molecular epidemiology of group a rotavirus in buenos aires, argentina 2004-2007: reemergence of g2p[4] and emergence of g9p[8] strains. | detection and characterization of group a rotavirus in buenos aires, argentina, was conducted on 710 fecal samples from children 0-15 years old collected between 2004 and 2007. rotavirus was detected in 140 (19.7%) samples with g9p[8] (30.0%) and g2p[4] (21.4%) as the most common genotypes. mixed (g and/or p) infections accounted for 17.9% of the samples and the emerging g12 strain was detected during 2004 (3.5%) and 2007 (2.5%). genotype g2 was the most prevalent during 2004 (43.9%) and 2007 (5 ... | 2010 | 20419826 |
| chemical and microbiological parameters as possible indicators for human enteric viruses in surface water. | there are still conflicting results on the suitability of chemical and microbiological parameters as indicators for the viral contamination of surface waters. in this study, conducted over 20 months, the abundance of human adenovirus, human polyomavirus, enterovirus, group a rotavirus and norovirus was determined in ruhr and rhine rivers, germany. additionally, prevalence of different possible indicators such as somatic coliphages, e. coli, intestinal enterococci, and total coliforms was also co ... | 2010 | 20556879 |
| [study on the epidemiological of rotavirus diarrhea in lulong in 2008-2009]. | to analyze the feature of epidemiological of rotavirus diarrhea in lulong county, hebei province. | 2010 | 20848835 |
| [expression and assembly of rotavirus-like particles in insect cells mediated by recombinant bombyx mori multibac]. | to construct recombinant baculoviruses co-expressing three structural genes vp2, vp6 and vp7 of rotavirus, and assemble rotavirus-like particles (vlps) in bmn cells. | 2010 | 20650749 |
| molecular characterization of unusual bovine group a rotavirus g8p[14] strains identified in western india: emergence of p[14] genotype. | a total of 78 fecal specimens were collected from both apparently healthy (n=71) and diarrheic (n=7) cattle from an organized farm in pune, western india in december 2007-january 2008. three specimens tested positive for group a rotavirus (rv) by antigen capture elisa were subjected to rt-pcr for amplification of entire coding regions of three structural (vp4, vp6 and vp7) and one nonstructural (nsp4) genes. all three strains were genotyped as g8p[14]. phylogenetic analysis of the vp7 and vp4 ge ... | 2010 | 20880637 |
| molecular characterization of human group a rotavirus from stool samples in young children with diarrhea in indonesia. | detection and genotyping of group a rotavirus strains from stool samples in young children with diarrhea in indonesia were examined using reverse transcription-nested multiplex pcr. of 421 stool specimens, 257 samples was rotavirus positive. g1 type was the most common g-type (54%), followed by g2 (6%) and g9 (3%). p[8] was the most common p-type (39%), followed by p[6] (19%), p[4] (10%) and p[11] 1%. eighteen percent of the samples had mixed g genotype infection and 5% had mixed p genotype infe ... | 2010 | 20578517 |
| complete genome constellation of a caprine group a rotavirus strain reveals common evolution with ruminant and human rotavirus strains. | this study reports the first complete genome sequence of a caprine group a rotavirus (gar) strain, go34. the vp7-vp4-vp6-vp1-vp2-vp3-nsp1-nsp2-nsp3-nsp4-nsp5 genes of strain go34, detected in bangladesh, were assigned to the g6-p[1]-i2-r2-c2-m2-a11-n2-t6-e2-h3 genotypes, respectively. strain go34 was closely related to the vp4, vp6-7 and nsp4-5 genes of bovine gars and the nsp1 gene of go34 to an ovine gar. strain go34 shared low nucleotide sequence identities (<90 %) with vp2-3 genes of other g ... | 2010 | 20505013 |
| molecular characterization of rotaviruses in a japanese raccoon dog (nyctereutes procyonoides) and a masked palm civet (paguma larvata) in japan. | group a rotaviruses infect and cause diarrhea in humans and a wide range of mammals. previous studies have suggested that some strains can cross the species barrier to infect humans (martella et al., 2010). however, there are few reports on infection and characterization of rotaviruses in wild animals. to estimate what types of rotaviruses infect wild animals, we investigated infection of rotaviruses in wild animals living in urban areas in japan between 2003 and 2008. of 145 fecal specimens obt ... | 2010 | 20605380 |
| identification of a g2-like porcine rotavirus bearing a novel vp4 type, p[32]. | a porcine group a rotavirus (garv) strain, 61/07/ire, was isolated from a 4-5 week asymptomatic piglet, during an epidemiological survey of porcine herds in southern ireland, in 2007. the nucleotide (nt) and amino acid (aa) sequence of the full-length vp4 protein of the porv strain 61/07/ire was determined. based on the entire vp4 open reading frame (nt), strain 61/07/ire displayed ≤76.5% identity to representatives of the established 31 p-types, a value far lower than the percentage identity cu ... | 2010 | 20663474 |
| analysis of genetic diversity and molecular evolution of human group b rotaviruses based on whole genome segments. | group b rotavirus (gbr) is a rare enteric pathogen that causes severe diarrhoea, primarily in adults. nearly full-length sequences of all 11 rna segments were determined for human gbrs detected recently in india (idh-084 in 2007, ic-008 in 2008), bangladesh (bang117 in 2003) and myanmar (mmr-b1 in 2007), and analysed phylogenetically with the sequence data of gbrs reported previously. all rna segments of gbr strains from india, bangladesh and myanmar showed >95 % nucleotide sequence identities. ... | 2010 | 20200192 |
| novel light-upon-extension real-time pcr assay for simultaneous detection, quantification, and genogrouping of group a rotavirus. | we have developed a light-upon-extension (lux) real-time pcr assay for detection, quantification, and genogrouping of group a rotavirus (rv), the most common cause of acute gastroenteritis in children. the lux system uses a fluorophore attached to one primer and having a self-quenching hairpin structure, making it cost-effective and specific. we designed genogroup-specific primers having different fluorophores, making it possible to differentiate between the two main genogroups of human group a ... | 2010 | 20220154 |
| prevalence and diversity of rotavirus strains in children with acute diarrhea from rural communities in the limpopo province, south africa, from 1998 to 2000. | data regarding the prevalence and molecular epidemiology of rotavirus infection in rural areas of africa are limited. in this study the prevalence and genetic diversity of rotaviruses in a rural south african setting were investigated. | 2010 | 20684695 |
| the genome segments of a group d rotavirus possess group a-like conserved termini but encode group-specific proteins. | rotaviruses are a leading cause of viral acute gastroenteritis in humans and animals. they are grouped according to gene composition and antigenicity of vp6. whereas group a, b, and c rotaviruses are found in humans and animals, group d rotaviruses have been exclusively detected in birds. despite their broad distribution among chickens, no nucleotide sequence data exist so far. here, the first complete genome sequence of a group d rotavirus (strain 05v0049) is presented, which was amplified usin ... | 2010 | 20631147 |
| concentration of acrylamide in a polyacrylamide gel affects vp4 gene coding assignment of group a equine rotavirus strains with p[12] specificity. | it is universally acknowledged that genome segment 4 of group a rotavirus, the major etiologic agent of severe diarrhea in infants and neonatal farm animals, encodes outer capsid neutralization and protective antigen vp4. | 2010 | 20573245 |
| immunogenicity and virus-like particle formation of rotavirus capsid proteins produced in transgenic plants. | the human pathogen, group a rotavirus, is the most prevalent cause of acute infantile and pediatric gastroenteritis worldwide, especially in developing countries. there is an urgent demand for safer, more effective and cheaper vaccines against rotavirus infection. plant-derived antigens may provide an exclusive way to produce economical subunit vaccines. virus-like particles, constituting viral capsid proteins without viral nucleic acids, are considered a far safer candidate compared with live a ... | 2010 | 21104033 |
| molecular analysis of human group a rotavirus g10p[14] genotype in slovenia. | rotavirus g10 genotype is one of the main rotaviruses circulating in cattle throughout the world but is also found in asymptomatic and symptomatic infections in children, and thought to be acquired through zoonotic transmission. | 2010 | 20675186 |
| comparative efficacy of immunological, molecular and culture assays for detection of group a rotavirus from faecal samples of buffalo (bubalus bubalis) calves. | group a rotaviruses play an important role in causing gastroenteritis and mortality in buffalo (bubalus bubalis) calves. a number of assays like rna-polyacrylamide gel electrophoresis (rna-page), enzyme-linked immunosorbent assay (elisa), reverse transcription polymerase chain reaction (rt-pcr) and virus isolation have been employed for rotavirus diagnosis. we evaluated the comparative efficacy of different assays for detection of group a rotavirus in buffalo calves. a total of 455 faecal sample ... | 2010 | 20607400 |
| genomic analysis of codon, sequence and structural conservation with selective biochemical-structure mapping reveals highly conserved and dynamic structures in rotavirus rnas with potential cis-acting functions. | rotaviruses are a major cause of acute, often fatal, gastroenteritis in infants and young children world-wide. virions contain an 11 segment double-stranded rna genome. little is known about the cis-acting sequences and structural elements of the viral rnas. using a database of 1621 full-length sequences of mammalian group a rotavirus rna segments, we evaluated the codon, sequence and rna structural conservation of the complete genome. codon conservation regions were found in eight orfs, suggest ... | 2010 | 20671030 |
| characterization of human rotavirus recovered from children with acute diarrhea in kinshasa, democratic republic of congo. | between july and october of 2003, 2004, and 2005, outbreaks of acute gastroenteritis occurred among children <5 years of age in kinshasa, democratic republic of the congo. stool specimens (67 in 2003, 108 in 2004, and 116 in 2005) were collected and screened for rotaviruses using either latex agglutination (diarlex laa; orion diagnostics) or enzyme immunoassay (ideia; dakocytomation). the molecular characteristics of the rotavirus strains were then determined. group a rotavirus was detected in 1 ... | 2010 | 20684702 |
| epidemiology of rotavirus infection in children in blantyre, malawi, 1997-2007. | acute gastroenteritis caused by rotavirus infection is an important cause of morbidity and mortality among infants and young children in africa. from 1997 through 2007, we enrolled 3740 children <5 years of age with acute gastroenteritis who received hospital care at the queen elizabeth central hospital in blantyre, malawi. group a rotavirus was detected in fecal specimens by enzyme immunoassay. rotavirus strains were characterized for vp7 (g) and vp4 (p) types with use of reverse-transcription ... | 2010 | 20684698 |
| molecular characterization of rotavirus strains circulating among children with acute gastroenteritis in madagascar during 2004-2005. | a survey was undertaken of the etiology of acute gastroenteritis in children <16 years of age in antananarivo, madagascar, from may 2004 through may 2005. with use of electron microscopy of fecal specimens, 104 (36%) of 285 children were found to be infected with rotavirus. rotavirus strain characterization was undertaken using enzyme-linked immunosorbent assay, electropherotyping, reverse-transcription polymerase chain reaction genotyping, and nucleotide sequencing. the predominant group a rota ... | 2010 | 20684699 |
| porcine kobuvirus from pig stool in korea. | to survey for porcine kobuvirus infection, a total of 119 pig fecal samples in korea were collected from three pig farms with good breeding facilities in three provinces. forty-three (36.1%) of the fecal samples tested were positive for porcine kobuvirus. in addition, porcine epidemic diarrhea virus (pedv) and transmissible gastroenteritis virus (tgev) infections were not identified in any of the fecal samples, but porcine group a rotavirus (gar) infection was identified in 11.8% (14/119) of the ... | 2010 | 21161358 |
| molecular characterization and genotyping of human rotavirus strains in abidjan, cote d'ivoire. | in this study, we characterized human rotavirus strains recovered from infants and young children with acute diarrhea in abidjan, cote d'ivoire, during 2000-2004. in total, 719 fecal specimens were collected from children aged 1-60 months with acute infantile gastroenteritis. examination with a commercial enzyme-linked immunosorbent assay showed the presence of group a rotavirus antigen in 208 diarrheal specimens (28.9%). polyacrylamide gel electrophoresis of the rna extracted from rotavirus-pos ... | 2010 | 20684706 |
| rotavirus strain diversity in the centre coast of tunisia from 2000 through 2003. | an epidemiological survey investigating rotavirus infection in children was undertaken in the coastal region of tunisia from january 2000 through september 2003. a total of 309 fecal specimens were screened by enzyme-linked immunosorbent assay and latex agglutination assay for the presence of group a rotavirus antigen. the detection rate was 26.2%. rotavirus outbreaks showed a temperature-dependant pattern (p = .026) but no significant association with rainfall. rotavirus strains isolated were a ... | 2010 | 20684712 |
| assessment of adenovirus, hepatitis a virus and rotavirus presence in environmental samples in florianopolis, south brazil. | to assess the presence of human adenovirus (hadv), hepatitis a (hav) virus and rotavirus a (rv-a) in environmental samples from the southern region of brazil and to provide viral contamination data for further epidemiological studies and governmental actions. | 2010 | 20698910 |
| multiplex semi-nested rt-pcr with exogenous internal control for simultaneous detection of bovine coronavirus and group a rotavirus. | neonatal calf diarrhea is a multi-etiology syndrome of cattle and direct detection of the two major agents of the syndrome, group a rotavirus and bovine coronavirus (bcov) is hampered by their fastidious growth in cell culture. this study aimed at developing a multiplex semi-nested rt-pcr for simultaneous detection of bcov (n gene) and group a rotavirus (vp1 gene) with the addition of an internal control (mrna nd5). the assay was tested in 75 bovine feces samples tested previously for rotavirus ... | 2010 | 20723564 |
| genomic diversity of group a rotavirus strains in patients aged 1-36 months admitted for acute watery diarrhoea in northern india: a hospital-based study. | the diversity and capacity of human rotaviruses for rapid evolution and genetic reassortment suggests that rotavirus vaccine should be designed to provide heterotypic protection. the objective of the present study was to provide information on the circulating genotypes of rotavirus and allied baseline epidemiology in lucknow, india. in a cross-sectional study, the prevalence of rotavirus g-p types was studied in patients aged 1-36 months with acute watery diarrhoea. various sociodemographic, env ... | 2010 | 19456832 |
| genotypic linkages of vp4, vp6, vp7, nsp4, nsp5 genes of rotaviruses circulating among children with acute gastroenteritis in thailand. | rotavirus is the main cause of acute viral gastroenteritis in infants and young children worldwide. surveillance of group a rotavirus has been conducted in chiang mai, thailand since 1987 up to 2004 and those studies revealed that group a rotavirus was responsible for about 20-61% of diarrheal diseases in hospitalized cases. in this study, we reported the continuing surveillance of group a rotavirus in 2005 and found that group a rotavirus was detected in 43 out of 147 (29.3%) stool samples. fiv ... | 2010 | 20223298 |
| rearranged genomic rna segments offer a new approach to the reverse genetics of rotaviruses. | group a rotaviruses (rv), members of the reoviridae family, are a major cause of infantile acute gastroenteritis. the rv genome consists of 11 double-stranded rna segments. in some cases, an rna segment is replaced by a rearranged rna segment, which is derived from its standard counterpart by partial sequence duplication. we report here a reverse genetics system for rv based on the preferential packaging of rearranged rna segments. using this system, wild-type or in vitro-engineered forms of rea ... | 2010 | 20427539 |
| viral load and genotypes of noroviruses in symptomatic and asymptomatic children in southeastern brazil. | noroviruses (novs) are a major etiological agent of sporadic acute gastroenteritis worldwide. | 2010 | 20004146 |
| molecular characterization of group a human rotavirus among hospitalized children and adults in bangladesh: finding of emerging g12 strain. | between july 2004 and june 2006, a total of 1438 fecal samples were collected from hospitalized children and adults with diarrhea at an infectious disease hospital (sk hospital) in mymensingh, bangladesh. the diversity of rotavirus strains were investigated using electropherotyping and reverse transcription-pcr amplification of the vp7 and vp4 genes. group a rotavirus was detected in 171 of 1438(11.9%) specimens: 13.3%(72/543) from children 6 months to </=15 years of age and 11.0%(99/895) from i ... | 2010 | 20046166 |
| characterization of group a rotavirus infections in adolescents and adults from pune, india: 1993-1996 and 2004-2007. | a total of 1,591 fecal specimens were collected in 1993-1996 and 2004-2007 from adolescents and adults with acute gastroenteritis in pune, india for detection and characterization of rotavirus. at the two time points, group a rotavirus was detected in 8.6% and 16.2% of the adolescents and 5.2% and 17.2% of the adults, respectively. reverse transcription-pcr with consensus primers followed by multiplex genotyping pcr detected common strains g1p[8], g2p[4], g3p[8], and g4p[8] in a total of 53.1% o ... | 2010 | 20087938 |
| prevalence of rotavirus in acute diarrhea and its association with clinical signs and symptoms. | rotaviruses are the main etiological agents of acute infectious diarrhea in children. thus, the objective of this study is to contribute to the information about the rotavirus variants circulating in brazil. a cross-sectional study was conducted on 124 fecal samples collected from children up to the age of 5 years, with acute gastroenteritis. thirty-one samples were positive for rotavirus a. regarding the g genotyping, 16 (80%) were classified as g1 and regarding p genotyping all strains were cl ... | 2010 | 19793895 |
| full genomic analysis and possible origin of a porcine g12 rotavirus strain ru172. | human group a rotavirus (gar) g12 strains are regarded as potentially important pathogens for acute gastroenteritis. on the other hand, to date, the only report of detection of g12 in animals was that of a porcine g12p[7] strain ru172. strain ru172 formed a separate g12 lineage, distinct from human g12 strains, and by analyses of deduced amino acid sequences, had a vp4, vp6, nsp4-5 of porcine origin. in the present study, we determined the full-length nucleotide sequences of vp1, vp3, and nsp1-3 ... | 2010 | 20157771 |
| genomic diversity among group a rotaviruses from diarrheic children, piglets, buffalo and cow calves of madhya pradesh. | diarrheal disease continues to be a global health problem, particularly among young ones in developing nations. amongst several viral and non-viral agents associated with diarrhea, group a rotavirus has been recognized as the major etiological agent of childhood gastroenteritis in human infants as well as several animal species throughout the world. during this study, a total of 181 diarrheic stool samples collected from children, piglets, buffalo and cow calves of madhya pradesh, central india ... | 2010 | 23100812 |
| instability of human rotavirus g genotypes circulating in a rural area of bangladesh. | a total of 280 fecal specimens from patients with acute gastroenteritis attending one rural hospital (dharmapasha health complex, sunamgonj) in bangladesh from august 2004 to may 2006 were tested for rotavirus by polyacrylamide gel electrophoresis (page). the diversity of rotavirus was investigated using electropherotyping and reverse transcription-pcr amplification of the vp7 and vp4 genes. the electrophoretic patterns of dsrna of rotavirus showed 9 different migrations (6 long and 3 short) by ... | 2011 | 21240155 |
| incidence assessment of rotavirus and adenovirus associated acute gastroenteritis cases in early childhood. | this study was undertaken to give an insight into the incidence of acute gastroenteritis cases of rotaviral/adenoviral aetiology in patients presenting to the emergency room of an inner-city government teaching hospital. group a rotavirus and adenovirus serotype 40-41 antigen results were obtained via immunochromatography. in 2007, there were 1543 patients with gastroenteritis between 0-5 years of age whose stool samples were tested for rota and adenovirus, of whom 386 (25%) had positive stool s ... | 2011 | 21753251 |
| burden and typing of rotavirus group a in latin america and the caribbean: systematic review and meta-analysis. | the efficacy of licensed rotavirus vaccines has only been shown against certain rotavirus group a (rv-a) types. it is critical to understand the burden of rotavirus gastroenteritis (rvge) and its prevalent types to assess the potential impact of these vaccines in latin america and the caribbean (la&c). we performed a systematic review and meta-analyses of all the available evidence reported from 1990 to 2009 on the burden of rotavirus disease and strains circulating in la&c. eligible studies-185 ... | 2011 | 21384462 |
| vp7 gene of human rotavirus a genotype g5: phylogenetic analysis reveals the existence of three different lineages worldwide. | group a rotavirus (rv-a) genotype g5, which is common in pigs, was also detected in children with severe diarrhea in brazil, argentina, paraguay, cameroon, china, thailand, and vietnam. to evaluate the evolutionary relationship among rv-a g5 strains, the vp7 and vp4 genes of 28 brazilian rv-a g5 human strains, sampled between 1986 and 2005, were sequenced and compared with other rv-a g5 strains currently circulating worldwide in animals and humans. the phylogenetic analysis of rv-a g5 vp7 gene s ... | 2011 | 21181934 |
| laboratory-based rotavirus surveillance during the introduction of a vaccination program, brazil, 2005-2009. | : brazil introduced universal antirotavirus vaccination in march 2006. this article reports the results of rotavirus a (rv-a) surveillance from january 2005 to december 2009. | 2011 | 21048523 |
| outbreak of rotavirus gastroenteritis in a nursing home, slovenia, december 2010. | a gastroenteritis outbreak affected 45 people (40 residents and five staff) in a nursing home for the elderly in the celje region, north-east slovenia, between 17 december and 31 december 2010. rotavirus group a was laboratory confirmed in the stools of five ill individuals. the outbreak was identified when the number of affected persons was high but was successfully controlled after implementing preventive measures. | 2011 | 21492527 |
| frequency, clinical characteristics, and genotype distribution of rotavirus gastroenteritis in greece (2007-2008). | rotavirus is the leading cause of acute gastroenteritis among young children worldwide. a prospective multi-center study was conducted (2007-2008) in five pediatric hospitals to determine the prevalence, the clinical characteristics, and genotype distribution of rotavirus infection in greece. faecal samples were examined for the presence of group a rotavirus antigen by immunochromatography. rotavirus strains were subjected to g and p genotyping by reverse-transcriptase polymerase chain reaction ... | 2011 | 21108355 |
| a waterborne outbreak of epidemic diarrhea due to group a rotavirus in malatya, turkey. | we characterized an outbreak of acute diarrheal disease caused by group a rotavirus that occurred during the autumn of 2005 in malatya city, turkey. a total 9907 patients between 0 to 91 years old (mean age: 25.05?19.67) were included in the epidemic. the patients? data were prospectively collected and statistically analyzed. microbiologic analyses were performed to determine the etiologic agent. rapid onset diarrhea (98.36%), abdominal cramps (69%), fever (44.4%) and vomiting (69.6%) were the m ... | 2011 | 21344142 |
| comparing electron microscopy and a competitive blocking elisa in the detection of rotaviruses in porcine faeces. | analysis of 476 faecal samples from diarrhoeic piglets was performed using electron microscopy (em) and a competitive blocking (cb)-elisa based on monoclonal antibodies to the vp6 protein of group a rotavirus. rotavirus was detected by em and/or cb-elisa in 111 (23.3%) samples. of these, all groups of rotavirus were identified in 83 (74.8%) samples by em (em+), while group a rotavirus was identified in 90 (81.1%) samples by cb-elisa (elisa+). however, only 62 (55.9%) of samples were positive usi ... | 2011 | 20036588 |
| viral and bacterial infections associated with camel (camelus dromedarius) calf diarrhea in north province, saudi arabia. | diarrhea and deaths in new-born camel calves were noticed by veterinary investigators and pastoralist in saudi arabia to be very high. hence, it is thought to be necessary to investigate this problem from the virological and bacteriological point of view. the role of pathogenic bacteria and viruses in six different towns of north province (al-assafia, arar, domat aljandal, hail, skaka and khoa) in saudi arabia was studied. survey was conducted in diarrheic camel calves aged 12 months or younger. ... | 2011 | 23961160 |
| bovine rotavirus strains circulating in beef and dairy herds in argentina from 2004 to 2010. | bovine group a rotavirus (rva) is one of the main causes of neonatal calf diarrhea worldwide. the present study reports the genotyping of bovine rva strains circulating in argentinean cattle from 2004 to 2010. additionally, a new set of typing primers was designed and tested to differentiate between g8 and g6 (lineage iii and iv) rva strains. bovine rva was detected in 30% (435/1462) of the tested samples, corresponding to 49% (207/423) of the studied outbreaks with a similar detection rates in ... | 2011 | 22503600 |