Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| hospital-based surveillance of rotavirus diarrhea in the people's republic of china, august 2003-july 2007. | rotaviruses cause acute diarrhea worldwide. previous studies of rotavirus diarrhea in china found that rotavirus infection is the most common cause of severe diarrhea in young children. in the present study, surveillance of rotavirus diarrhea was conducted involving 9549 children aged <5 years who were admitted for treatment of diarrhea at 11 sentinel hospitals in china from august 2003 through july 2007. group a rotavirus was detected in 3749 (47.8%) of the 7846 fecal specimens by using enzyme- ... | 2009 | 19817597 |
| hospital-based surveillance of rotavirus and other viral agents of diarrhea in children and adults in russia, 2005-2007. | during a 2-year period in 2005-2007, we conducted surveillance of group a rotaviruses and other enteric agents among patients hospitalized with acute gastroenteritis in 8 different cities of the russian federation. fecal specimens were gathered from 3208 children (including 2848 children aged <5 years) and 1354 adults who were admitted to hospitals in moscow, st. petersburg, chelyabinsk, nizhnii novgorod, tyumen, khabarovsk, makhachkala, and yakutsk. polymerase chain reaction was performed to de ... | 2009 | 19817602 |
| rotavirus surveillance in europe, 2005-2008: web-enabled reporting and real-time analysis of genotyping and epidemiological data. | the first european rotavirus surveillance network, eurorotanet, comprising 16 laboratories in 15 european countries, has been established. | 2009 | 19821712 |
| [sequence and evolutionary analysis of vp1 gene of ovine rotavirus nt]. | the ovine rotavirus strain nt isolated from diarrhea lamb in china was considered as a promising vaccine strain. based on the vp1 gene was one of the important structural proteins of rotavirus, we studied on the evolutional characteristics of vp1. | 2009 | 19835167 |
| distribution of g (vp7) and p (vp4) genotypes of group a bovine rotaviruses from turkish calves with diarrhea, 1997-2008. | group a rotaviruses are major enteric pathogens of calves. in order to investigate the genetic diversity of bovine rotaviruses (brvs), a collection of 53 brvs, detected from diarrheic calves from several turkish geographical areas, between 1997 and 2008 was analyzed by rt-pcr for specificities of the outer capsid proteins vp7 (g type) and vp4 (p type), for the first time. overall, g6 was the predominant g type, detected in 40/53 samples (75.4%), while p[11] was the predominant p type, detected i ... | 2010 | 19854003 |
| relationship among epidemiological parameters of six childhood infections in a non-immunized brazilian community. | epidemiological parameters, such as age-dependent force of infection and average age at infection (<img src='../img/13s1.gif' width=9 height=12>) were estimated for rubella, varicella, rotavirus a, respiratory syncytial virus, hepatitis a and parvovirus b19 infections for a non-immunized brazilian community, using the same sera samples. the for the aforementioned diseases were 8.45 years (yr) [95% ci: (7.23, 9.48) yr], 3.90 yr [95% ci: (3.51, 4.28) yr], 1.03 yr [95% ci: (0.96, 1.09) yr], 1.58 yr ... | 2009 | 19876563 |
| culturing, storage, and quantification of rotaviruses. | group a rotaviruses (rvs) infect the young of numerous animal species and cause acute gastroenteritis. cultivation of animal and human rvs in cells requires proteolytic activation of the viral attachment protein using trypsin. continuous cell lines, such as rhesus monkey kidney cells, as well as primary monkey kidney cells, are routinely used for the growth and characterization of rvs. isolation and cultivation of human rvs from clinical fecal specimens is difficult and adaptation to growth in v ... | 2009 | 19885940 |
| [evolution of group a rotavirus strains circulating in tunisia over a 3-year period (2005-2007).] | background: rotaviruses are the most frequent agents associated with diarrhoea in children worldwide. analysis of mobility of the 11 segments of genomic rna by polyacrylamide gel electrophoresis (page) yields a pattern which is characteristic for a particular rotavirus isolate. the group a rotaviruses can be further characterized by analysis of vp7 and vp4 genes specificities, responsible for rotavirus classification into g and p genotypes, respectively. the aim of the present study was to deter ... | 2009 | 19896286 |
| rotac: a web-based tool for the complete genome classification of group a rotaviruses. | group a rotaviruses are the most common cause of severe diarrhea in infants and children worldwide and continue to have a major global impact on childhood morbidity and mortality. in recent years, considerable research efforts have been devoted to the development of two new live, orally administered vaccines. although both vaccines have proven to confer a good protection against severe rotavirus gastroenteritis, these vaccines will have to be screened and may have to be updated regularly to refl ... | 2009 | 19930627 |
| molecular characterization of the vp1, vp2, vp4, vp6, nsp1 and nsp2 genes of bovine group b rotaviruses: identification of a novel vp4 genotype. | studies on bovine group b rotaviruses (gbrs) are limited. to date, only the vp6 gene of a single bovine gbr strain and the vp7 and nsp5 genes of a few bovine gbr strains have been sequenced and analyzed. in the present study, using a single-primer amplification method, we have determined the full-length nucleotide sequences of the vp1, vp2, vp4, vp6, nsp1 and nsp2 genes of three bovine gbr strains from eastern india. in all six of these genes, the bovine gbr strains shared high genetic relatedne ... | 2010 | 19936611 |
| [nsp5/nsp6 of rotavirus strain tb-chen and preliminary genotyping analysis]. | strain tb-chen is a group a rotavirus (rv) isolated from a chinese infant suffering from gastroenteritis in hospital. the nsp5 and nsp6 of strain tb-chen are encoded by the 10th gene segment (816bp in whole length) of the viral genome. the results obtained in this study showed that the nsp5 was encoded in the first open-reading-frame (orf) of the gene segment (from 22bp to 624bp), and nsp6 was encoded in the second orf (from 80bp to 355bp). the nsp5 protein consisted of 200 amino acid residues w ... | 2009 | 19954111 |
| viral load and genotypes of noroviruses in symptomatic and asymptomatic children in southeastern brazil. | noroviruses (novs) are a major etiological agent of sporadic acute gastroenteritis worldwide. | 2010 | 20004146 |
| analysis of rotavirus non-structural protein nsp5 by mass spectrometry reveals a complex phosphorylation pattern. | genomic replication and partial assembly of rotavirus takes place in cytoplasmic viral structures called viroplasms. nsp5 is a viral phosphoprotein localized in viroplasms and its expression is imperative for viral cycle progress. during infection three isoforms of nsp5 can be observed by sds-page (26, 28 and 33-35kda) and previous reports suggested that they differ in their phosphorylation patterns. in this study we obtained nsp5 from infected cells and by mass spectrometry we were able to iden ... | 2010 | 20036292 |
| comparing electron microscopy and a competitive blocking elisa in the detection of rotaviruses in porcine faeces. | analysis of 476 faecal samples from diarrhoeic piglets was performed using electron microscopy (em) and a competitive blocking (cb)-elisa based on monoclonal antibodies to the vp6 protein of group a rotavirus. rotavirus was detected by em and/or cb-elisa in 111 (23.3%) samples. of these, all groups of rotavirus were identified in 83 (74.8%) samples by em (em+), while group a rotavirus was identified in 90 (81.1%) samples by cb-elisa (elisa+). however, only 62 (55.9%) of samples were positive usi ... | 2011 | 20036588 |
| molecular characterization of group a human rotavirus among hospitalized children and adults in bangladesh: finding of emerging g12 strain. | between july 2004 and june 2006, a total of 1438 fecal samples were collected from hospitalized children and adults with diarrhea at an infectious disease hospital (sk hospital) in mymensingh, bangladesh. the diversity of rotavirus strains were investigated using electropherotyping and reverse transcription-pcr amplification of the vp7 and vp4 genes. group a rotavirus was detected in 171 of 1438(11.9%) specimens: 13.3%(72/543) from children 6 months to </=15 years of age and 11.0%(99/895) from i ... | 2010 | 20046166 |
| characterization of group a rotavirus infections in adolescents and adults from pune, india: 1993-1996 and 2004-2007. | a total of 1,591 fecal specimens were collected in 1993-1996 and 2004-2007 from adolescents and adults with acute gastroenteritis in pune, india for detection and characterization of rotavirus. at the two time points, group a rotavirus was detected in 8.6% and 16.2% of the adolescents and 5.2% and 17.2% of the adults, respectively. reverse transcription-pcr with consensus primers followed by multiplex genotyping pcr detected common strains g1p[8], g2p[4], g3p[8], and g4p[8] in a total of 53.1% o ... | 2010 | 20087938 |
| phylogenetic analysis of human p[8]g9 rotavirus strains circulating in brazil reveals the presence of a novel genetic variant. | group a rotavirus (rv-a) genotype p[8]g9 has emerged as one of the leading causes of gastroenteritis in children worldwide and currently is recognized as one of the five most common genotypes detected in humans. high intragenotype diversity in g9 rv-a has been observed, and nowadays, based on the genetic variability of the vp7 gene, six different phylogenetic lineages and eleven sublineages were described. | 2010 | 20133185 |
| full genomic analysis and possible origin of a porcine g12 rotavirus strain ru172. | human group a rotavirus (gar) g12 strains are regarded as potentially important pathogens for acute gastroenteritis. on the other hand, to date, the only report of detection of g12 in animals was that of a porcine g12p[7] strain ru172. strain ru172 formed a separate g12 lineage, distinct from human g12 strains, and by analyses of deduced amino acid sequences, had a vp4, vp6, nsp4-5 of porcine origin. in the present study, we determined the full-length nucleotide sequences of vp1, vp3, and nsp1-3 ... | 2010 | 20157771 |
| genetic variation of g4p[6] rotaviruses: evidence for novel strains circulating between the hospital and community. | one hundred forty-six fecal specimens collected between 2007 and 2008 from infants with acute gastroenteritis were screened for rotavirus by elisa with vp6-specific antibody. one hundred twenty-three of the samples (84.2%) were confirmed to be positive for group a rotavirus (community-acquired, n = 90 [73.2%] and nosocomial, n = 33 [26.8%]), and were typed subsequently using rt-pcr and sequence analysis methods. determination of g- and p-type combinations showed that g4p[6] (78.9%) was the most ... | 2010 | 20166174 |
| circulating human group a rotavirus genotypes in malaysia. | this study examined the temporal distribution of rotavirus genotypes in malaysia. rotaviruses from children with diarrhea admitted to hospitals in 1996 (n = 93) and 2007 (n = 12) in two different regions of peninsular (west) malaysia were analyzed for their g and p genotypes using a hemi-nested rt-pcr assay. in the 2007 samples, the dominant strain was g9p[8]. it was identified in 42% of the samples. different strains all possessing the g1 genotype were identified in the rest of the samples. in ... | 2010 | 20166178 |
| multiplex nested rt-pcr for the detection of porcine enteric viruses. | porcine epidemic diarrhea virus (pedv), transmissible gastroenteritis virus (tgev), and porcine group a rotavirus (prv-a) are major viruses causing enteric diseases of piglets. a multiplex nested reverse transcription polymerase chain reaction (multiplex nested rt-pcr) was developed for the detection of these viruses in field samples from piglets with diarrhea. a mixture of (1) three external pairs of primers, yielding in the amplification step two different amplicons with sizes of 950 bp and 31 ... | 2010 | 20170679 |
| rotavirus-associated diarrhoea in foals in greece. | severe outbreaks of diarrhoeic syndrome occurred in young foals at the same stud farm during two consecutive breeding periods namely spring 2006 and 2007. rotavirus-like particles were detected by electron microscopy in the faeces of the affected foals and group a rotavirus infection was confirmed by reverse-transcription (rt)-pcr with selected sets of rotavirus-specific primers. sequence analysis of the genes encoding the outer capsid rotavirus proteins vp7 and vp4 enabled classification of the ... | 2010 | 20197218 |
| rotavirus nsp5 orchestrates recruitment of viroplasmic proteins. | rotavirus genome replication and the first steps of virus morphogenesis take place in cytoplasmic viral factories, called viroplasms, containing four structural (vp1, vp2, vp3 and vp6) and two non-structural (nsp2 and nsp5) proteins. nsp2 and nsp5 have been shown to be essential for viroplasm formation and, when co-expressed in uninfected cells, to form viroplasm-like structures (vls). in the present work, vls formation was shown upon co-expression of nsp5 with the core protein vp2 despite the a ... | 2010 | 20200190 |
| analysis of genetic diversity and molecular evolution of human group b rotaviruses based on whole genome segments. | group b rotavirus (gbr) is a rare enteric pathogen that causes severe diarrhoea, primarily in adults. nearly full-length sequences of all 11 rna segments were determined for human gbrs detected recently in india (idh-084 in 2007, ic-008 in 2008), bangladesh (bang117 in 2003) and myanmar (mmr-b1 in 2007), and analysed phylogenetically with the sequence data of gbrs reported previously. all rna segments of gbr strains from india, bangladesh and myanmar showed >95 % nucleotide sequence identities. ... | 2010 | 20200192 |
| novel light-upon-extension real-time pcr assay for simultaneous detection, quantification, and genogrouping of group a rotavirus. | we have developed a light-upon-extension (lux) real-time pcr assay for detection, quantification, and genogrouping of group a rotavirus (rv), the most common cause of acute gastroenteritis in children. the lux system uses a fluorophore attached to one primer and having a self-quenching hairpin structure, making it cost-effective and specific. we designed genogroup-specific primers having different fluorophores, making it possible to differentiate between the two main genogroups of human group a ... | 2010 | 20220154 |
| genotypic linkages of vp4, vp6, vp7, nsp4, nsp5 genes of rotaviruses circulating among children with acute gastroenteritis in thailand. | rotavirus is the main cause of acute viral gastroenteritis in infants and young children worldwide. surveillance of group a rotavirus has been conducted in chiang mai, thailand since 1987 up to 2004 and those studies revealed that group a rotavirus was responsible for about 20-61% of diarrheal diseases in hospitalized cases. in this study, we reported the continuing surveillance of group a rotavirus in 2005 and found that group a rotavirus was detected in 43 out of 147 (29.3%) stool samples. fiv ... | 2010 | 20223298 |
| rotavirus antigenemia and genomia in children with rotavirus gastroenteritis. | we investigated group a rotavirus (garv) antigenemia and genomia in children with rotavirus gastroenteritis. a total of 16 patients (2-29 months old), who received a diagnosis of garv gastroenteritis using a commercial rapid test, were enrolled in this study. the sera from the patients were tested for the presence of garv antigen and the vp7 and nsp3 genes using an enzyme-linked immunosorbent assay (elisa) and reverse transcription-polymerase chain reaction, respectively. furthermore, when the v ... | 2010 | 20332567 |
| geographical differences in the proportion of human group a rotavirus strains within new zealand during one epidemic season. | the prospect of future rotavirus vaccine programs means it is important to understand rotavirus strain diversity within new zealand, especially if this was to influence vaccine effectiveness. the g-genotype of 359 group a rotavirus strains isolated from 416 stool samples collected from june 2005 to may 2006 (inclusive) from children less than 5 years of age in multiple centers throughout new zealand was determined. g1 was the dominant circulating strain (55.8%) followed by g4 (21.4%), g3 (3.4%), ... | 2010 | 20336723 |
| detection and genotyping of korean porcine rotaviruses. | porcine group a rotavirus (garv) is considered to be an important animal pathogen due to their economic impact in the swine industry and its potential to cause heterologous infections in humans. this study examined 475 fecal samples from 143 farms located in 6 provinces across south korea. rt-pcr and nested pcr utilizing primer pairs specific for the garv vp6 gene detected garv-positive reactions in 182 (38.3%) diarrheic fecal samples. a total of 98 porcine garv strains isolated from the garv-po ... | 2010 | 20359834 |
| genetic characterization of norovirus gii.4 2006b variants from jeju island, south korea. | ninety-seven fecal specimens collected from children with acute gastroenteritis between 2007 and 2008 that were found to be negative for group a rotavirus in prescreening by elisa with vp6-specific antibody were re-screened for viruses by reverse transcription (rt)-pcr. forty (41.2%) samples were found to be positive for virus by rt-pcr; of these, norovirus (32.5%, n = 13) and rotavirus (32.5%, n = 13) were the most common, followed by astrovirus (5.0%, n = 2) and enterovirus (2.5%, n = 1). co-i ... | 2010 | 20419823 |
| molecular epidemiology of group a rotavirus in buenos aires, argentina 2004-2007: reemergence of g2p[4] and emergence of g9p[8] strains. | detection and characterization of group a rotavirus in buenos aires, argentina, was conducted on 710 fecal samples from children 0-15 years old collected between 2004 and 2007. rotavirus was detected in 140 (19.7%) samples with g9p[8] (30.0%) and g2p[4] (21.4%) as the most common genotypes. mixed (g and/or p) infections accounted for 17.9% of the samples and the emerging g12 strain was detected during 2004 (3.5%) and 2007 (2.5%). genotype g2 was the most prevalent during 2004 (43.9%) and 2007 (5 ... | 2010 | 20419826 |
| rearranged genomic rna segments offer a new approach to the reverse genetics of rotaviruses. | group a rotaviruses (rv), members of the reoviridae family, are a major cause of infantile acute gastroenteritis. the rv genome consists of 11 double-stranded rna segments. in some cases, an rna segment is replaced by a rearranged rna segment, which is derived from its standard counterpart by partial sequence duplication. we report here a reverse genetics system for rv based on the preferential packaging of rearranged rna segments. using this system, wild-type or in vitro-engineered forms of rea ... | 2010 | 20427539 |
| noroviruses as cause of gastroenteritis in elderly patients. | background and aims. gastroenteritis is one of the infections that are particularly important to the elderly people. the knowledge of the main pathogens causing gastroenteritis in this group of patients, whose number will be dramatically increased in coming decades, is essential. the contribution of group a rotavirus, adenovirus type 41 and 42, norovirus and astrovirus as cause of gastroenteritis among patients of all ages, especially those with more than 65 years old over an extended time perio ... | 2010 | 20460955 |
| complete genome constellation of a caprine group a rotavirus strain reveals common evolution with ruminant and human rotavirus strains. | this study reports the first complete genome sequence of a caprine group a rotavirus (gar) strain, go34. the vp7-vp4-vp6-vp1-vp2-vp3-nsp1-nsp2-nsp3-nsp4-nsp5 genes of strain go34, detected in bangladesh, were assigned to the g6-p[1]-i2-r2-c2-m2-a11-n2-t6-e2-h3 genotypes, respectively. strain go34 was closely related to the vp4, vp6-7 and nsp4-5 genes of bovine gars and the nsp1 gene of go34 to an ovine gar. strain go34 shared low nucleotide sequence identities (<90 %) with vp2-3 genes of other g ... | 2010 | 20505013 |
| detection of uncommon rotavirus a strains p[8]g8 and p[4]g8 in the city of rio de janeiro, 2002. | group a rotaviruses (rv-a) are the major cause of gastroenteritis in infants and young children around the world. each year rv-a causes approximately 11 million episodes of severe diarrhea, with an estimated of 611,000 deaths. epidemiologic surveys have identified p[8]g1, p[4]g2, p[8]g3, p[8]g4, and p[8]g9 as the most common global genotypes associated with diarrhea in children up to 5-year old. surveillance studies and documentation of rv-a g and p genotypes is necessary for a comprehensive eva ... | 2010 | 20513095 |
| correlation between rotavirus a genotypes detected in hospitalized children and sewage samples in 2006, córdoba, argentina. | routine rotavirus a (rv-a) surveillance is based on clinical cases, so only symptomatic infections are reported. the objective of this study was to determine whether the rv-a genotypes and cold seasonal pattern described in patients with diarrhea is reflected by sewage surveillance, which could be representative of the rv-a genotypes circulating in the population. the genotype distribution of rv-a in effluent samples from a local sewage treatment plant was compared to those from local clinical c ... | 2010 | 20513096 |
| detection and characterisation of group a rotavirus in asymptomatic piglets in southern ireland. | porcine group a rotaviruses (garv) are causative agents of enteritis in piglets and are a large reservoir of genetic material for the diversification of human garvs. accumulation of information on the genetic heterogeneity of porcine viruses is pivotal for readily characterising unusual human strains. screening of 292 fecal samples, collected from 4-5- to 8-9-week-old asymptomatic pigs from four herds in ireland between 2005 and 2007 resulted in 19 (6.5%) samples testing positive by reverse-tran ... | 2010 | 20526785 |
| molecular characterization and epidemiology of rotavirus isolates obtained from children with diarrhoea in malaysia. | this retrospective study examined the g/p type of rotavirus in rna samples that have previously been e-typed by rna-page in 1996. the results were then compared to 2007 samples to ascertain the extent of changes that may have occurred in this 11-years time interval. the g and p genotypes were determined by hemi-nested pcr and further analysed by phylogenetic study. in 1996, the g/p combination g1p[8], g(ut)p[8] and g1p(ut) prevalence rate were 81%, 9% and 7%, respectively. as expected, the g9 ge ... | 2009 | 20527266 |
| changing profile of the bovine rotavirus g6 population in the south of ireland from 2002 to 2009. | bovine group a rotavirus is one of the main causes of neonatal diarrhoea in calves. this study examined the different g and p genotypes circulating in the bovine population, from 2002-2009, in the south of ireland. rotavirus positive bovine faecal samples (n=332) were collected from the cork regional veterinary laboratory, between 2002 and 2009 and subjected to rna extraction, page analysis, and g and p genotyping. genotyping analysis identified g6, g10, p[5], and p[11] to be the predominant g a ... | 2010 | 20541335 |
| detection and molecular characterization of enteric viruses in environmental samples in monastir, tunisia between january 2003 and april 2007. | a prospective study was performed to characterize the main human enteric viruses able to persist in sewage samples and in shellfish tissues, and to establish the correlation between environmental strains and viral infantile diarrhoea observed in the same area during the same period. | 2010 | 20553345 |
| chemical and microbiological parameters as possible indicators for human enteric viruses in surface water. | there are still conflicting results on the suitability of chemical and microbiological parameters as indicators for the viral contamination of surface waters. in this study, conducted over 20 months, the abundance of human adenovirus, human polyomavirus, enterovirus, group a rotavirus and norovirus was determined in ruhr and rhine rivers, germany. additionally, prevalence of different possible indicators such as somatic coliphages, e. coli, intestinal enterococci, and total coliforms was also co ... | 2010 | 20556879 |
| concentration of acrylamide in a polyacrylamide gel affects vp4 gene coding assignment of group a equine rotavirus strains with p[12] specificity. | it is universally acknowledged that genome segment 4 of group a rotavirus, the major etiologic agent of severe diarrhea in infants and neonatal farm animals, encodes outer capsid neutralization and protective antigen vp4. | 2010 | 20573245 |
| molecular characterization of human group a rotavirus from stool samples in young children with diarrhea in indonesia. | detection and genotyping of group a rotavirus strains from stool samples in young children with diarrhea in indonesia were examined using reverse transcription-nested multiplex pcr. of 421 stool specimens, 257 samples was rotavirus positive. g1 type was the most common g-type (54%), followed by g2 (6%) and g9 (3%). p[8] was the most common p-type (39%), followed by p[6] (19%), p[4] (10%) and p[11] 1%. eighteen percent of the samples had mixed g genotype infection and 5% had mixed p genotype infe ... | 2010 | 20578517 |
| molecular characterization of rotaviruses in a japanese raccoon dog (nyctereutes procyonoides) and a masked palm civet (paguma larvata) in japan. | group a rotaviruses infect and cause diarrhea in humans and a wide range of mammals. previous studies have suggested that some strains can cross the species barrier to infect humans (martella et al., 2010). however, there are few reports on infection and characterization of rotaviruses in wild animals. to estimate what types of rotaviruses infect wild animals, we investigated infection of rotaviruses in wild animals living in urban areas in japan between 2003 and 2008. of 145 fecal specimens obt ... | 2010 | 20605380 |
| comparative efficacy of immunological, molecular and culture assays for detection of group a rotavirus from faecal samples of buffalo (bubalus bubalis) calves. | group a rotaviruses play an important role in causing gastroenteritis and mortality in buffalo (bubalus bubalis) calves. a number of assays like rna-polyacrylamide gel electrophoresis (rna-page), enzyme-linked immunosorbent assay (elisa), reverse transcription polymerase chain reaction (rt-pcr) and virus isolation have been employed for rotavirus diagnosis. we evaluated the comparative efficacy of different assays for detection of group a rotavirus in buffalo calves. a total of 455 faecal sample ... | 2010 | 20607400 |
| chemical and microbiological parameters as possible indicators for human enteric viruses in surface water. | there are still conflicting results on the suitability of chemical and microbiological parameters as indicators for the viral contamination of surface waters. in this study, conducted over 20 months, the abundance of human adenovirus, human polyomavirus, enterovirus, group a rotavirus and norovirus was determined in ruhr and rhine rivers, germany. additionally, prevalence of different possible indicators such as somatic coliphages, e. coli, intestinal enterococci, and total coliforms was also co ... | 2010 | 20627679 |
| the genome segments of a group d rotavirus possess group a-like conserved termini but encode group-specific proteins. | rotaviruses are a leading cause of viral acute gastroenteritis in humans and animals. they are grouped according to gene composition and antigenicity of vp6. whereas group a, b, and c rotaviruses are found in humans and animals, group d rotaviruses have been exclusively detected in birds. despite their broad distribution among chickens, no nucleotide sequence data exist so far. here, the first complete genome sequence of a group d rotavirus (strain 05v0049) is presented, which was amplified usin ... | 2010 | 20631147 |
| [expression and assembly of rotavirus-like particles in insect cells mediated by recombinant bombyx mori multibac]. | to construct recombinant baculoviruses co-expressing three structural genes vp2, vp6 and vp7 of rotavirus, and assemble rotavirus-like particles (vlps) in bmn cells. | 2010 | 20650749 |
| identification of a g2-like porcine rotavirus bearing a novel vp4 type, p[32]. | a porcine group a rotavirus (garv) strain, 61/07/ire, was isolated from a 4-5 week asymptomatic piglet, during an epidemiological survey of porcine herds in southern ireland, in 2007. the nucleotide (nt) and amino acid (aa) sequence of the full-length vp4 protein of the porv strain 61/07/ire was determined. based on the entire vp4 open reading frame (nt), strain 61/07/ire displayed ≤76.5% identity to representatives of the established 31 p-types, a value far lower than the percentage identity cu ... | 2010 | 20663474 |
| genomic analysis of codon, sequence and structural conservation with selective biochemical-structure mapping reveals highly conserved and dynamic structures in rotavirus rnas with potential cis-acting functions. | rotaviruses are a major cause of acute, often fatal, gastroenteritis in infants and young children world-wide. virions contain an 11 segment double-stranded rna genome. little is known about the cis-acting sequences and structural elements of the viral rnas. using a database of 1621 full-length sequences of mammalian group a rotavirus rna segments, we evaluated the codon, sequence and rna structural conservation of the complete genome. codon conservation regions were found in eight orfs, suggest ... | 2010 | 20671030 |
| molecular analysis of human group a rotavirus g10p[14] genotype in slovenia. | rotavirus g10 genotype is one of the main rotaviruses circulating in cattle throughout the world but is also found in asymptomatic and symptomatic infections in children, and thought to be acquired through zoonotic transmission. | 2010 | 20675186 |
| prevalence and diversity of rotavirus strains in children with acute diarrhea from rural communities in the limpopo province, south africa, from 1998 to 2000. | data regarding the prevalence and molecular epidemiology of rotavirus infection in rural areas of africa are limited. in this study the prevalence and genetic diversity of rotaviruses in a rural south african setting were investigated. | 2010 | 20684695 |
| epidemiology of rotavirus infection in children in blantyre, malawi, 1997-2007. | acute gastroenteritis caused by rotavirus infection is an important cause of morbidity and mortality among infants and young children in africa. from 1997 through 2007, we enrolled 3740 children <5 years of age with acute gastroenteritis who received hospital care at the queen elizabeth central hospital in blantyre, malawi. group a rotavirus was detected in fecal specimens by enzyme immunoassay. rotavirus strains were characterized for vp7 (g) and vp4 (p) types with use of reverse-transcription ... | 2010 | 20684698 |
| molecular characterization of rotavirus strains circulating among children with acute gastroenteritis in madagascar during 2004-2005. | a survey was undertaken of the etiology of acute gastroenteritis in children <16 years of age in antananarivo, madagascar, from may 2004 through may 2005. with use of electron microscopy of fecal specimens, 104 (36%) of 285 children were found to be infected with rotavirus. rotavirus strain characterization was undertaken using enzyme-linked immunosorbent assay, electropherotyping, reverse-transcription polymerase chain reaction genotyping, and nucleotide sequencing. the predominant group a rota ... | 2010 | 20684699 |
| characterization of human rotavirus recovered from children with acute diarrhea in kinshasa, democratic republic of congo. | between july and october of 2003, 2004, and 2005, outbreaks of acute gastroenteritis occurred among children <5 years of age in kinshasa, democratic republic of the congo. stool specimens (67 in 2003, 108 in 2004, and 116 in 2005) were collected and screened for rotaviruses using either latex agglutination (diarlex laa; orion diagnostics) or enzyme immunoassay (ideia; dakocytomation). the molecular characteristics of the rotavirus strains were then determined. group a rotavirus was detected in 1 ... | 2010 | 20684702 |
| molecular characterization and genotyping of human rotavirus strains in abidjan, cote d'ivoire. | in this study, we characterized human rotavirus strains recovered from infants and young children with acute diarrhea in abidjan, cote d'ivoire, during 2000-2004. in total, 719 fecal specimens were collected from children aged 1-60 months with acute infantile gastroenteritis. examination with a commercial enzyme-linked immunosorbent assay showed the presence of group a rotavirus antigen in 208 diarrheal specimens (28.9%). polyacrylamide gel electrophoresis of the rna extracted from rotavirus-pos ... | 2010 | 20684706 |
| rotavirus strain diversity in the centre coast of tunisia from 2000 through 2003. | an epidemiological survey investigating rotavirus infection in children was undertaken in the coastal region of tunisia from january 2000 through september 2003. a total of 309 fecal specimens were screened by enzyme-linked immunosorbent assay and latex agglutination assay for the presence of group a rotavirus antigen. the detection rate was 26.2%. rotavirus outbreaks showed a temperature-dependant pattern (p = .026) but no significant association with rainfall. rotavirus strains isolated were a ... | 2010 | 20684712 |
| assessment of adenovirus, hepatitis a virus and rotavirus presence in environmental samples in florianopolis, south brazil. | to assess the presence of human adenovirus (hadv), hepatitis a (hav) virus and rotavirus a (rv-a) in environmental samples from the southern region of brazil and to provide viral contamination data for further epidemiological studies and governmental actions. | 2010 | 20698910 |
| multiplex semi-nested rt-pcr with exogenous internal control for simultaneous detection of bovine coronavirus and group a rotavirus. | neonatal calf diarrhea is a multi-etiology syndrome of cattle and direct detection of the two major agents of the syndrome, group a rotavirus and bovine coronavirus (bcov) is hampered by their fastidious growth in cell culture. this study aimed at developing a multiplex semi-nested rt-pcr for simultaneous detection of bcov (n gene) and group a rotavirus (vp1 gene) with the addition of an internal control (mrna nd5). the assay was tested in 75 bovine feces samples tested previously for rotavirus ... | 2010 | 20723564 |
| detection of rotavirus a in sewage samples using multiplex qpcr and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration. | group a rotaviruses (rv-a) are the most common agents of viral gastroenteritis in children worldwide. the goal of this study was to compare two different methods to concentrate rv-a from sewage samples and to improve the detection and quantification of rv-a using a multiplex quantitative pcr assay with an internal control. both rv-a and the internal control virus, bacteriophage pp7, were seeded into wastewater and then concentrated using either an ultrafiltration-based adsorption-elution protoco ... | 2010 | 20804786 |
| multiple-gene characterization of rotavirus strains: evidence of genetic linkage among the vp7-, vp4-, vp6-, and nsp4-encoding genes. | a total of 162 rotavirus strains detected between 1996 and 2006 among individuals with diarrhea in rio de janeiro, brazil, were analyzed by multiple-gene genotyping. characterization of strains was done by rt-pcr assay for amplification and typing of the vp7-, vp4-, vp6-, and nsp4-encoding genes. overall, 139 (85.8%) strains belonged to the common group a rotavirus combinations: 67 (41.4%) belonged to genotype g1-p[8]-i1-e1; 18 (11.1%) were g2-p[4]-i2-e2; 11 (6.8%) were g3-p[8]-i1-e1; 12 (7.4%) ... | 2010 | 20827779 |
| diagnosis of viral gastroenteritis by simultaneous detection of adenovirus group f, astrovirus, rotavirus group a, norovirus genogroups i and ii, and sapovirus in two internally controlled multiplex real-time pcr assays. | norovirus, rotavirus group a, astrovirus, sapovirus and adenovirus serotypes 40 and 41, are common causes of gastroenteritis. conventional diagnosis of these causative agents is based on antigen detection and electron microscopy. | 2010 | 20829103 |
| outbreak of acute gastroenteritis in young children with death due to rotavirus genotype g9 in rio branco, brazilian amazon region, 2005. | an epidemic of acute gastroenteritis occurred in rio branco city, acre state, in brazil's amazon region in 2005. an investigation was conducted to confirm the etiology and identify possible risk factors for death. | 2010 | 20843719 |
| [study on the epidemiological of rotavirus diarrhea in lulong in 2008-2009]. | to analyze the feature of epidemiological of rotavirus diarrhea in lulong county, hebei province. | 2010 | 20848835 |
| determination of g and p type diversity of group a rotaviruses in faecal samples of diarrhoeic calves in kashmir, india. | to determine the genetic diversity of group a rotaviruses in bovine calves in kashmir, india. | 2010 | 20875036 |
| molecular characterization of unusual bovine group a rotavirus g8p[14] strains identified in western india: emergence of p[14] genotype. | a total of 78 fecal specimens were collected from both apparently healthy (n=71) and diarrheic (n=7) cattle from an organized farm in pune, western india in december 2007-january 2008. three specimens tested positive for group a rotavirus (rv) by antigen capture elisa were subjected to rt-pcr for amplification of entire coding regions of three structural (vp4, vp6 and vp7) and one nonstructural (nsp4) genes. all three strains were genotyped as g8p[14]. phylogenetic analysis of the vp7 and vp4 ge ... | 2010 | 20880637 |
| comparison of the rapid methods for screening of group a rotavirus in stool samples. | in this study, the sensitivity and specificity of three immunochromatography (ic) test kits for rapid detection of group a rotavirus were compared and evaluated with stool samples collected from children, who suffered from acute gastroenteritis during february to june, 2009 in japan. a total of 86 stool samples were tested and compared with a reference rt-pcr method. the sensitivity among ip-rota v, dipstick eiken rota and rota-adeno test kits were 97.2, 95.8 and 88.7%, while the specificity wer ... | 2010 | 21030457 |
| full genomic analysis of a simian sa11-like g3p[2] rotavirus strain isolated from an asymptomatic infant: identification of novel vp1, vp6 and nsp4 genotypes. | we report here the full genomic analysis of a simian sa11-like g3p[2] group a rotavirus (gar) strain, b10, isolated from an asymptomatic infant in kenya in 1987. by nucleotide sequence identities and phylogenetic analyses, the vp7-vp4-vp2-vp3-nsp1-nsp2-nsp3-nsp5 genes of strain b10 exhibited maximum genetic relatedness to those of the different isolates of simian strain sa11, and were assigned to the g3-p[2]-c5-m5-a5-n5-t5-h5 genotypes, respectively. on the other hand, the vp1, vp6 and nsp4 gene ... | 2010 | 21035567 |
| quantification and molecular characterization of enteric viruses detected in effluents from two hospital wastewater treatment plants. | hospital wastewater has been described as an important source of spreading pathogenic microorganisms in the environment. however, there are few studies reporting the presence and concentrations of gastroenteric viruses and hepatitis a viruses in these environmental matrices. the aim of this study was to assess the contamination by viruses responsible for acute gastroenteritis and hepatitis derived from hospital wastewater treatment plants (wwtps). rotavirus a (rv-a), human adenoviruses (hadv), n ... | 2010 | 21040941 |
| [detection of bovine rotavirus g10p[11] in a diary farm in daqing, china]. | group a rotavirus are the most frequently detected viral agent associated with the acute diarrhea in calves. in order to investigate the situation of rotavirus strains circulating in diary farms, a total of 117 fecal specimens were collected from diarrhea calves under 4 weeks-age on yinluo diary farm in daqing region in china from 2008 to 2009. ten specimens were detected to be positive by a rotavirus group a diagnostic kit, which confirmed that the rotavirus was important viral agent associated ... | 2010 | 21043143 |
| laboratory-based rotavirus surveillance during the introduction of a vaccination program, brazil, 2005-2009. | : brazil introduced universal antirotavirus vaccination in march 2006. this article reports the results of rotavirus a (rv-a) surveillance from january 2005 to december 2009. | 2011 | 21048523 |
| [epidemiological surveillance of norovirus and rotavirus diarrhea among outpatient children in five metropolitan cities]. | to survey the clinical epidemiological features of norovirus and rotavirus diarrhea among children living in 5 cities. | 2010 | 21055297 |
| a hospital based study on inter- and intragenotypic diversity of human rotavirus a vp4 and vp7 gene segments, germany. | efforts to reduce the impact of group a rotaviruses on human morbidity and mortality rely on oral immunisation with live attenuated or recombinant vaccines. a major challenge in immunisation is the vast inter- and intragenotypic diversity accomplished by circulating rotaviruses. | 2010 | 21074491 |
| predominance of porcine p[23] genotype rotaviruses in piglets with diarrhea in northern thailand. | of 131 stool samples collected from piglets with diarrhea in northern thailand between july 2006 and august 2008, 14 (10.7%) were positive for group a rotavirus. sequence analysis showed that 13 strains (92.9%) belonged to the rare p[23] genotype combination with g9 or g3 genotypes. | 2010 | 21084504 |
| [rotavirus prevalence in infants and children in the public healthcare system of the state of pernambuco]. | rotaviruses are considered important etiological agents of acute gastroenteritis and a common cause of the hospitalization of children aged zero to four years-old. in brazil, the incidence of rotavirus gastroenteritis in children is 12 to 42% and the distribution of infection is related to seasonality, which apparently occurs in different periods and intensity according to each region. the study investigated group a rotavirus in fecal samples of suspected children attended by the public health s ... | 2010 | 21085867 |
| immunogenicity and virus-like particle formation of rotavirus capsid proteins produced in transgenic plants. | the human pathogen, group a rotavirus, is the most prevalent cause of acute infantile and pediatric gastroenteritis worldwide, especially in developing countries. there is an urgent demand for safer, more effective and cheaper vaccines against rotavirus infection. plant-derived antigens may provide an exclusive way to produce economical subunit vaccines. virus-like particles, constituting viral capsid proteins without viral nucleic acids, are considered a far safer candidate compared with live a ... | 2010 | 21104033 |
| frequency, clinical characteristics, and genotype distribution of rotavirus gastroenteritis in greece (2007-2008). | rotavirus is the leading cause of acute gastroenteritis among young children worldwide. a prospective multi-center study was conducted (2007-2008) in five pediatric hospitals to determine the prevalence, the clinical characteristics, and genotype distribution of rotavirus infection in greece. faecal samples were examined for the presence of group a rotavirus antigen by immunochromatography. rotavirus strains were subjected to g and p genotyping by reverse-transcriptase polymerase chain reaction ... | 2011 | 21108355 |
| reassortant group a rotavirus from straw-colored fruit bat (eidolon helvum). | bats are known reservoirs of viral zoonoses. we report genetic characterization of a bat rotavirus (bat/ke4852/07) detected in the feces of a straw-colored fruit bat (eidolon helvum). six bat rotavirus genes (viral protein [vp] 2, vp6, vp7, nonstructural protein [nsp] 2, nsp3, and nsp5) shared ancestry with other mammalian rotaviruses but were distantly related. the vp4 gene was nearly identical to that of human p[6] rotavirus strains, and the nsp4 gene was closely related to those of previously ... | 2010 | 21122212 |
| epidemiology and clinical features of gastroenteritis in hospitalised children: prospective survey during a 2-year period in a parisian hospital, france. | rotavirus is recognised as the most important agent of severe acute gastroenteritis (age) in young children. in a 2-year prospective survey, we investigated the epidemiology and clinical features of the viral and bacterial pathogens in children hospitalised for age. the study was performed in a parisian teaching hospital from november 2001 to may 2004. clinical data were prospectively collected to assess the gastroenteritis severity (20-point vesikari severity score, the need for intravenous reh ... | 2010 | 21128089 |
| genome sequence based molecular epidemiology of unusual us rotavirus a g9 strains isolated from omaha, usa between 1997 and 2000. | after discovery in the early 1980s, rotavirus a serotype g9 was detected infrequently for almost a decade. since the mid-1990s, however, serotype g9 has emerged to become a globally common strain linked to the introduction of a single, new genetic variant of g9 vp7 gene. studies have demonstrated that genetically divergent g9 strains co-circulated at low frequency with the emerging variants. examples include unique u.s. g9 strains om46/hu/usa/1998 and om67/hu/usa/1998, isolated in omaha during t ... | 2010 | 21130184 |
| porcine kobuvirus from pig stool in korea. | to survey for porcine kobuvirus infection, a total of 119 pig fecal samples in korea were collected from three pig farms with good breeding facilities in three provinces. forty-three (36.1%) of the fecal samples tested were positive for porcine kobuvirus. in addition, porcine epidemic diarrhea virus (pedv) and transmissible gastroenteritis virus (tgev) infections were not identified in any of the fecal samples, but porcine group a rotavirus (gar) infection was identified in 11.8% (14/119) of the ... | 2010 | 21161358 |
| vp7 gene of human rotavirus a genotype g5: phylogenetic analysis reveals the existence of three different lineages worldwide. | group a rotavirus (rv-a) genotype g5, which is common in pigs, was also detected in children with severe diarrhea in brazil, argentina, paraguay, cameroon, china, thailand, and vietnam. to evaluate the evolutionary relationship among rv-a g5 strains, the vp7 and vp4 genes of 28 brazilian rv-a g5 human strains, sampled between 1986 and 2005, were sequenced and compared with other rv-a g5 strains currently circulating worldwide in animals and humans. the phylogenetic analysis of rv-a g5 vp7 gene s ... | 2011 | 21181934 |
| full genomic analysis of a g8p[1] rotavirus strain isolated from an asymptomatic infant in kenya provides evidence for an artiodactyl-to-human interspecies transmission event. | group a rotavirus (gar) g8p[1] strains, found sometimes in cattle, have been reported rarely from humans. therefore, analysis of the full genomes of human g8p[1] strains are of significance in the context of studies on interspecies transmission of rotaviruses. however, to date, only partial-length nucleotide sequences are available for the 11 genes of a single human g8p[1] strain, while the partial sequences of two other strains have been reported. the present study reports the first complete ge ... | 2011 | 21181935 |
| increased sensitivity for various rotavirus genotypes in stool specimens by amending three mismatched nucleotides in the forward primer of a real-time rt-pcr assay. | the real-time taqman rt-pcr assay (pang et al., 2004) did not detect 14 clinical samples with rotavirus g2 genotype. three to five nucleotides (nt) were found to be mismatched between the published forward primer when compared to g2p[4], g2p[8], g3p[4], g9p[4], g8 and g12 sequences. an additional forward primer was designed and included in a modified assay to test the 14 clinical samples and 12 samples with known rotavirus g and p genotypes. the modified assay has improved significantly the sens ... | 2010 | 21185331 |
| human rotavirus genotypes detection among hospitalized children, a study in tehran, iran. | human rotavirus genotypes g1-g4, g9, p[4] and p[8] are major worldwide causes of acute gastroenteritis in children. rotavirus genotype g1p[8] is predominant in many countries. in this study, the genotypic diversity of group a rotaviruses were detected in children <5 years of age who were treated for dehydration and diarrhea in tehran, iran from october 2004 to september 2008. | 2011 | 21194260 |
| novel group a rotavirus g8 p[1] as primary cause of an ovine diarrheic syndrome outbreak in weaned lambs. | rotavirus is a worldwide major cause of diarrhea outbreaks in neonatal ruminants. an outbreak of ovine diarrheic syndrome (ods) in 50-75 days-old lambs (weaned lambs) is described. fecal immunochromatography and intestinal immunohistochemistry for rotavirus group a were performed. in addition, semi-nested multiplex rt-pcr for g and p rotavirus genotyping in combination with sequencing were performed, to support the diagnosis and identify the viral strain. a novel ovine rotavirus group a g8 p[1] ... | 2010 | 21216110 |
| rotavirus a genotype g1p[8]: a novel method to distinguish wild-type strains from the rotarix vaccine strain. | rotaviruses are important enteric pathogens for humans and animals. group a rotaviruses (rv-a) are the most common agents of severe gastroenteritis in infants and young children and vaccination is the most effective method to reduce rv-a-associated diseases. g1p[8], the most prevalent rv-a genotype worldwide, is included in the rv-a vaccine rotarix®. the discrimination between wild-type g1p[8] and vaccine g1p[8] strains is an important topic in the study of rv-a epidemiology to manage outbreaks ... | 2010 | 21225209 |
| instability of human rotavirus g genotypes circulating in a rural area of bangladesh. | a total of 280 fecal specimens from patients with acute gastroenteritis attending one rural hospital (dharmapasha health complex, sunamgonj) in bangladesh from august 2004 to may 2006 were tested for rotavirus by polyacrylamide gel electrophoresis (page). the diversity of rotavirus was investigated using electropherotyping and reverse transcription-pcr amplification of the vp7 and vp4 genes. the electrophoretic patterns of dsrna of rotavirus showed 9 different migrations (6 long and 3 short) by ... | 2011 | 21240155 |
| analysis of human p[4]g2 rotavirus strains isolated in brazil reveals codon usage bias and strong compositional constraints. | the rotavirus genus belongs to the family reoviridae and its genome consist of 11 segments of double-stranded rna. group a rotaviruses (rv-a) are the main etiological agent of acute viral gastroenteritis in infants and young children worldwide. understanding the extent and causes of biases in codon usage is essential to the understanding of viral evolution. however, the factors shaping synonymous codon usage bias and nucleotide composition in human rv-a are currently unknown. in order to gain in ... | 2011 | 21255687 |
| development of a combined immunomagnetic separation and quantitative reverse transcription-pcr assay for sensitive detection of infectious rotavirus in water samples. | a quantitative and rapid detection method for rotavirus in water samples was developed using immunomagnetic separation combined with quantitative reverse transcription-polymerase chain reaction (ims-rt-qpcr). magnetic beads coated with antibodies against representative group a rotavirus were used to capture and purify intact rotavirus particles in both artificial and real environmental water sample matrix. compared to extracting rna using commercial kits and rt-qpcr assay, the developed ims-rt-q ... | 2011 | 21256895 |
| highly sensitive detection of the group a rotavirus using apolipoprotein h-coated elisa plates compared to quantitative real-time pcr. | the principle of a capture elisa is binding of specific capture antibodies (polyclonal or monoclonal) to the surface of a suitable 96 well plate. these immobilized antibodies are capable of specifically binding a virus present in a clinical sample. subsequently, the captured virus is detected using a specific detection antibody. the drawback of this method is that a capture elisa can only function for a single virus captured by the primary antibody. human apolipoprotein h (apoh) or ß2-glycoprote ... | 2011 | 21310042 |
| epidemiology of group a rotaviruses surveillance and burden of disease studies. | human infection has been reported with groups a, b, and c rotaviruses (rvs). of these, group a rvs are the most important, being a major cause of severe gastroenteritis (ge). each year, group a rvs are estimated to cause approx 870,000 deaths worldwide in children less than 5 years (yr) of age, mostly in developing countries (1). this chapter will describe the epidemiological features of group a rv infections, and will critically review the current surveillance strategies used to define the burd ... | 2000 | 21318862 |
| a waterborne outbreak of epidemic diarrhea due to group a rotavirus in malatya, turkey. | we characterized an outbreak of acute diarrheal disease caused by group a rotavirus that occurred during the autumn of 2005 in malatya city, turkey. a total 9907 patients between 0 to 91 years old (mean age: 25.05?19.67) were included in the epidemic. the patients? data were prospectively collected and statistically analyzed. microbiologic analyses were performed to determine the etiologic agent. rapid onset diarrhea (98.36%), abdominal cramps (69%), fever (44.4%) and vomiting (69.6%) were the m ... | 2011 | 21344142 |
| a single-tube multiplex pcr for rapid detection in feces of 10 viruses causing diarrhea. | a novel multiplex polymerase chain reaction assay was developed to identify 10 viruses in a single tube. the assay was targeted to detect group a and c rotaviruses, adenovirus, norovirus gi, norovirus gii, sapovirus, astrovirus, aichi virus, parechovirus, and enterovirus. a total of 235 stool samples were collected from infants and children with acute gastroenteritis in kyoto, japan, from 2008 to 2009, then tested by this novel multiplex pcr and compared with a multiplex pcr described previously ... | 2011 | 21349292 |
| burden and typing of rotavirus group a in latin america and the caribbean: systematic review and meta-analysis. | the efficacy of licensed rotavirus vaccines has only been shown against certain rotavirus group a (rv-a) types. it is critical to understand the burden of rotavirus gastroenteritis (rvge) and its prevalent types to assess the potential impact of these vaccines in latin america and the caribbean (la&c). we performed a systematic review and meta-analyses of all the available evidence reported from 1990 to 2009 on the burden of rotavirus disease and strains circulating in la&c. eligible studies-185 ... | 2011 | 21384462 |
| molecular characterization of group a rotaviruses detected in children with gastroenteritis in ireland in 2006-2009. | summarycommunity and hospital-acquired cases of human rotavirus are responsible for millions of gastroenteritis cases in children worldwide, chiefly in developing countries, and vaccines are now available. during surveillance activity for human rotavirus infections in ireland, between 2006 and 2009, a total of 420 rotavirus strains were collected and analysed. upon either pcr genotyping and sequence analysis, a variety of vp7 (g1-g4 and g9) and vp4 (p[4], p[6], p[8] and p[9]) genotypes were dete ... | 2011 | 21396145 |
| [annual incidence of human group a rotavirus g-serotypes detected in a japanese university hospital between 2003 and 2007]. | group a rotavirus g-serotyping by polymerase chain reaction (pcr) using university hospital subject samples in september 2003 to august 2004, september 2004 to august 2005, september 2005 to august 2006, and september 2006 to august 2007 showed the most common serotypes g1 and g3, detected in 27 and 33 subjects, compared to 4 subjects in whom serotype g4 was detected. between 2003 and 2004, serotypes g1 accounted for 50% and g3 for 38%, contrasting with serotype g3 at 79% between 2004 and 2005, ... | 2011 | 21404601 |
| outbreak of rotavirus gastroenteritis in a nursing home, slovenia, december 2010. | a gastroenteritis outbreak affected 45 people (40 residents and five staff) in a nursing home for the elderly in the celje region, north-east slovenia, between 17 december and 31 december 2010. rotavirus group a was laboratory confirmed in the stools of five ill individuals. the outbreak was identified when the number of affected persons was high but was successfully controlled after implementing preventive measures. | 2011 | 21492527 |
| monitoring of group a rotaviruses in wild-living birds in hungary. | rotavirus is a common pathogen causing gastroenteritis in humans and domesticated animals. the incidence of rotavirus in wild-living animals, particularly in avian species, has not been systematically investigated. in this study 1220 fecal samples and cloacal swabs collected from wild-living birds during 2008 in hungary were tested for the presence of group a rotaviruses by a vp6 gene-specific reverse-transcription-polymerase-chain-reaction assay. of the 1220 samples, 276 and 944 were processed ... | 2011 | 21500648 |
| rotavirus a genotype p[4]g2: genetic diversity and reassortment events among strains circulating in brazil between 2005 and 2009. | group a rotaviruses (rv-a) are the leading cause of severe gastroenteritis in infants and young children worldwide. due to the epidemiologic complexity of rv-a, especially in developing countries, it is important to determine which genotypes are circulating, principally after the introduction in march 2006 of the monovalent (p[8]g1) rotarix® vaccine in brazil by the national immunization program. in phase iii trials with rotarix®, the prevalence of genotype p[4]g2 was extremely low, and therefor ... | 2011 | 21503926 |