Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| a one-year intensified study of outbreaks of gastroenteritis in the netherlands. | in 2002, in the netherlands a national study of gastroenteritis outbreaks was performed. epidemiological information was collected by the public health services (phs) and the food inspection services (fis) using standardized questionnaires. stool samples were collected for diagnostic testing. for foodborne outbreaks, food samples were taken. in total, 281 gastroenteritis outbreaks were included, mainly from nursing homes and homes for the elderly (57%), restaurants (11%), hospitals (9%) and day- ... | 2005 | 15724705 |
| dominating prevalence of p[8],g1 and p[8],g9 rotavirus strains among children admitted to hospital between 2000 and 2003 in budapest, hungary. | group a rotaviruses are the main cause of acute dehydrating diarrhea in children, responsible for high mortality in developing countries and a significant socio-economic burden associated with treating the disease in developed countries. two rotavirus vaccine candidates predicated on either homotypic or heterotypic protection have undergone clinical trials recently and await licensure for routine use. in anticipation of a future vaccination campaign in hungary, the diversity of rotaviruses colle ... | 2005 | 15902709 |
| design and selection of an intrabody library produced de-novo for the non-structural protein nsp5 of rotavirus. | intracellular antibodies or intrabodies have great potential in protein knockout strategies for intracellular antigens. we applied the intracellular antibody capture technology for the direct selection in yeast of a mouse scfv library (v(l)-v(h) format) constructed from animals immunised with recombinant non-structural protein nsp5 of rotavirus. we selected five different intracellular antibodies (icabs), which specifically recognize delta2, an nsp5 deletion mutant used as bait. the anti-nsp5 ic ... | 2005 | 15907924 |
| sequence analysis of the vp7 and vp4 genes identifies a novel vp7 gene allele of porcine rotaviruses, sharing a common evolutionary origin with human g2 rotaviruses. | during an epidemiological survey encompassing several porcine herds in saragoza, spain, the vp7 and vp4 of a rotavirus-positive sample, 34461-4, could not be predicted by using multiple sets of g- and p-type-specific primers. sequence analysis of the vp7 gene revealed a low amino acid (aa) identity with those of well-established g serotypes, ranging between 58.33% and 88.88%, with the highest identity being to human g2 rotaviruses. analysis of the vp4 gene revealed a p[23] vp4 specificity, as it ... | 2005 | 15914225 |
| identification of the novel lapine rotavirus genotype p[22] from an outbreak of enteritis in a hungarian rabbitry. | application of improved molecular techniques in the detection and characterization of rotavirus strains has led to the recent description of several new combinations, specificities, and genetic variants of the outer capsid genes, vp7 and vp4. in spite of the enormous diversity of mammalian rotavirus strains, the few lapine rotaviruses characterized to date, appear to carry a narrow range of such antigen combinations; only p[14], g3 and, based on a more recent study, p[22], g3 rotaviruses have pr ... | 2005 | 15936106 |
| oral immunization with pbsvp6-transgenic alfalfa protects mice against rotavirus infection. | a critical factor in edible plant-derived vaccine development is adequate expression of the exogenous antigens in transgenic plants. we synthesized a codon-optimized gene (svp6) encoding the vp6 protein of human group a rotavirus and inserted it into the alfalfa genome using agrobacterium-mediated transformation. as much as 0.28% of the total soluble protein of the pbsvp6-transgenic alfalfa was svp6. female balb/c mice were gavaged weekly with 10 mg of transgenic alfalfa extract containing 24 mi ... | 2005 | 15992851 |
| serological characterization of novel p11[14],g8 bovine group a rotavirus, sun9, isolated in japan. | in this study, a novel bovine group a rotavirus (borv-a), sun9, isolated from calf diarrhea in tochigi prefecture, japan, was serologically characterized by a cross-neutralization assay, and serological surveillance by using its reassortant was performed on cattle bred in japan. the g serotype of sun9 was identified as g serotype 8 based on the one- or two-way serological relationships observed in sun9 and other g8 strains. the p serotype of sun9 was identified as p serotype 11 based on the one- ... | 2005 | 15996788 |
| [optimization of rt-pcr for the identification of vp4 gene of group a rotaviruses and evaluation of its diagnostic efficiency]. | a method is suggested for the detection of the human rotavirus vp4 gene by rt-pcr. the method is universal for all [p] types of group a rotavirus in clinical samples. as against rna electrophoresis and elisa, it provides for a more accurate detection of rotaviruses by 18.8 and 26.5%, respectively. it was established by rt-pcr in february, 2002, in nizhny novgorod, that rotaviruses were responsible for 67% of acute gastroenteritis in hospitalized children. | 2005 | 15747872 |
| predominance of porcine rotavirus g9 in japanese piglets with diarrhea: close relationship of their vp7 genes with those of recent human g9 strains. | type g9 of group a rotavirus (gar) was shown to be predominant in a survey of vp7 (g) and vp4 (p) genotypes among porcine gars associated with outbreaks of diarrhea in young pigs in japan between 2000 and 2002. comparison of the g9 vp7 gene sequences showed that the porcine g9 strains were more closely related to human g9 strains reemerging globally since the mid-1990s than to those from the mid-1980s. the vp7 gene sequences of porcine g9 strains from different farms were divergent (6.1 to 7.2% ... | 2005 | 15750112 |
| detection of unusual strains of rv in patients with acute diarrhoea in mexico. | group a rotaviruses are a major cause of acute gastroenteritis in infants. human strains with a short rna pattern generally exhibit subgroup i, g2, p1b[4] specificity, those with a long rna pattern show subgroup ii, g1, g3 or g4, p1a[8] specificity. the presence of strains with unusual specificities has been reported worldwide over the last decade. | 2005 | 15780813 |
| molecular characterization of the rotavirus nsp4 enterotoxin homologue from group b rotavirus. | the rna segment (gene 10) from a human group b rotavirus which encodes the homologue of the rotavirus enterotoxin (nsp4) has been cloned and sequenced. the gene is of the same length (751 nucleotides) as its better-characterized group a rotavirus counterpart but shows minimal homology (approximately 10%) to it at the primary sequence level. despite this low level of sequence homology, secondary structure predictions for the group b protein (adrv-nsp4) showed a close similarity of structural feat ... | 2005 | 15845266 |
| viral diarrhea in japanese children: results from a one-year epidemiologic study. | a total of 557 fecal specimens from infants and children with acute gastroenteritis in five places (maizuru, tokyo, sapporo, saga and osaka) in japan from july 2002 to june 2003 were tested for the presence of diarrheal viruses by rt-pcr, prha, rna-page and latex agglutination methods. of these, 56.4% (314) were found positive for diarrheal viruses. among them, group a rotavirus was the most prevalent (43.6%, 137 of 314) followed by norovirus (29.9%, 94 of 314), adenovirus (7.6%, 24 of 314), gro ... | 2005 | 15819174 |
| viral etiology and incidence of acute gastroenteritis in young children attending day-care centers. | the purpose of the study was to investigate the frequency, morbidity and cause of acute gastroenteritis in children attending day-care centers in denmark. | 2005 | 16282929 |
| characterization of a novel p[25],g11 human group a rotavirus. | a novel rotavirus strain (dhaka6) isolated from a 21-year-old bangladeshi male patient was characterized by sequence analysis of its vp7 and vp4 gene segments. phylogenetic analysis of the vp7 gene of the dhaka6 strain revealed a common evolutionary lineage with porcine g11 rotavirus strains. this isolate is the first reported g11 rotavirus strain infecting a human host. comparison of the vp4 gene sequences with all currently recognized 24 different p genotypes revealed only low nucleotide (54 t ... | 2005 | 16000437 |
| the incidence of rotavirus a isolates of g genotype in thailand in 2002-2004. | rotaviruses are the leading cause of severe gastroenteritis among infants and young children worldwide. between november 2002 and march 2004, 36 stool specimens of 108 children with acute diarrhea in bangkok, thailand were found positive for rotavirus a (rv-a) by rt-pcr. the 36 isolates were subjected to genotyping by rflp analysis and direct sequencing of a part of the gene for major outer capsid glycoprotein vp7. the sequences obtained were subjected to phylogenetic analysis. among the isolate ... | 2005 | 16047738 |
| distribution of g (vp7) and p (vp4) genotypes in buffalo group a rotaviruses isolated in southern italy. | group a rotaviruses are established agents of disease in buffalo calves. early epidemiological studies in italian buffalo herds revealed the predominance of strains with g8 specificity and detected strains with the rare, rrv-like, vp4 p[3] genotype. to acquire additional information on the vp4 and vp7 specificities of buffalo rotaviruses, a total of 125 fecal samples were collected from buffalo calves affected with diarrhoea, in seven dairy farms in southern italy. rotaviruses were detected in 2 ... | 2005 | 16137843 |
| detection and characterization of human group c rotaviruses in bangladesh. | group c rotaviruses were detected by reverse transcription-pcr in 14 (2.3%) of 611 group a rotavirus-negative stool specimens from the patients admitted to the international centre for diarrhoeal disease research, bangladesh hospital, dhaka, bangladesh, during july to december 2003. the low rate of detection suggested that infection with group c rotaviruses was an uncommon cause of hospitalization due to gastroenteritis. in addition, coinfections with pathogenic enteric bacteria were frequently ... | 2005 | 16145092 |
| the tissue micro-array data exchange specification: a web based experience browsing imported data. | the aids and cancer specimen resource (acsr) is an hiv/aids tissue bank consortium sponsored by the national cancer institute (nci) division of cancer treatment and diagnosis (dctd). the acsr offers to approved researchers hiv infected biologic samples and uninfected control tissues including tissue cores in micro-arrays (tma) accompanied by de-identified clinical data. researchers interested in the type and quality of tma tissue cores and the associated clinical data need an efficient method fo ... | 2005 | 16086837 |
| rotaviruses interact with alpha4beta7 and alpha4beta1 integrins by binding the same integrin domains as natural ligands. | group a rotaviruses are major intestinal pathogens that express potential alpha4beta1 and alpha4beta7 integrin ligand sequences leu-asp-val and leu-asp-ile in their outer capsid protein vp7, and ile-asp-ala in their spike protein vp4. monkey rotavirus sa11 can use recombinant alpha4beta1 as a cellular receptor. in this study a new potential alpha4beta1, alpha4beta7 and alpha9beta1 integrin ligand sequence, tyr-gly-leu, was identified in vp4. it was shown that several human and monkey rotaviruses ... | 2005 | 16298987 |
| isolation, identification and characterization of group a rotavirus from a chicken: the inner capsid protein sequence shows only a distant phylogenetic relationship to most other avian group a rotaviruses. | rotavirus particles were identified in the intestinal content of a 35-day-old stunted chicken. the virus was isolated, rna pattern was analysed and the viral genome segment 6 was sequenced. in particular, the sequence data showed a very close similarity to the chicken rotavirus isolate ch-1 (99.2% amino acid homology), this is distantly related to all known avian rotaviruses and supports the existence of different vp6 types amongst avian group a rotaviruses. | 2005 | 16115093 |
| isolation and molecular characterization of a naturally occurring non-structural protein 5 (nsp5) gene reassortant of group a rotavirus of serotype g2p[4] with a long rna pattern. | we report here two unusual strains of group a rotavirus, au85 and au102, isolated from children with diarrhea. these strains showed an unusual combination of serotype g2 and a long rna pattern. rna-rna hybridization assays showed that these strains are reassortants in which a single genome segment 11 (the nsp5 gene) was derived from a wa genogroup strain, while other 10 genome segments from a ds-1 genogroup strain. phylogenetic analysis showed that the nsp5 gene of strain au85 did not form clust ... | 2005 | 16121367 |
| detection and molecular characterisation of group a rotavirus from children in northern botswana. | to determine the presence of group a rotavirus in human stool samples in northern botswana and to characterise the circulating strains. | 2005 | 16122089 |
| development of a novel protocol for rt-multiplex pcr to detect diarrheal viruses among infants and children with acute gastroenteritis in eastern russia. | viral gastroenteritis is one of the most common illnesses in humans worldwide and it has a great impact on people. recently, we reported three rt-multiplex pcr assays termed a, b and c that could detect three groups of diarrheal viruses; group a, b and c rotaviruses and adenovirus [phan et al., j med virol 2004; 74:173-9]; norovirus gi and gii, sapovirus and astrovirus [yan et al., j virol meth 2003; 114:37-44]; enteroviruses, hepatitis a and e viruses and influenza a virus [phan et al., arch vi ... | 2005 | 16122155 |
| molecular characterization of the major capsid protein vp6 of bovine group b rotavirus and its use in seroepidemiology. | the major inner capsid protein (vp6) gene of the bovine group b rotavirus (gbr) nemuro strain is 1269 nt in length and contains one open reading frame encoding 391 aa. nucleotide and amino acid sequence identities of the nemuro vp6 gene compared with the published corresponding human and rodent gbr genes were respectively 66-67 and 70-72 %, which are notably lower than those between human and rodent viruses (72-73 and 83-84 %, respectively). overall identities of vp6 genes among gbrs were substa ... | 2005 | 16099916 |
| [expression of recombinant vp6 gene of porcine rotavirus a with non-antibiotic lactobacillus vector in escherichia coli]. | the antigenic determinants of vp6 gene of porcine rotavirus a was amplified from infected ma 104 cell by the reverse transcription-polymerase chain reaction (rt-pcr), the product of which was a 1194bp cdna segment. using t-a cloning technique, the pcr product was cloned into pgem-t vector. cloning plasmid pgem-t-vp6 and the prokaryotic shuttle expression vector pw425t between e. coli and lactobacillus, were digested by saci and kpni double enzymes, respectively. the purified vp6 gene was subclon ... | 2005 | 16342756 |
| emergence of human g9 rotavirus with an exceptionally high frequency in children admitted to hospital with diarrhea in chiang mai, thailand. | among 315 fecal specimens collected from children hospitalized with diarrhea in chiang mai, thailand, in 2000-2001, group a rotavirus was detected in 107 (34.0%). of these, 98 (91.6%) were g9, 6 (5.6%) were g3 and 3 (2.8%) were g2, respectively. identification of their p-types demonstrated that 103 (96.3%) were p[8], 3 (2.8%) were p[4], and 1 (0.9%) was p[3] genotypes. determination of g- and p-type combination revealed that all of g9 isolates were associated with p[8]. g9p[8] was the most predo ... | 2006 | 16372282 |
| etiology and epidemiology of diarrhea in children in hanoi, vietnam. | this paper provides a preliminary picture of diarrhea with regards to etiology, clinical symptoms, and some related epidemiologic factors in children less than five years of age living in hanoi, vietnam. | 2006 | 16458564 |
| molecular characterization of a porcine group a rotavirus strain with g12 genotype specificity. | a porcine group a rotavirus strain (ru172) was detected and molecularly characterized during a surveillance study conducted for rotavirus infection in a pig farm located in a suburban area of kolkata city, india. the g12 genotype specificity of ru172 was revealed by pcr-based genotyping assays following addition of a g12 type-specific primer (designed in our laboratory to pick up g12 isolates from field samples) and was confirmed by sequence analysis of the vp7-encoding gene. the ru172 strain ex ... | 2006 | 16502286 |
| g8 rotavirus strains isolated in the democratic republic of congo belong to the ds-1-like genogroup. | several g8p[6] and g8p[8] rotavirus strains were isolated from hospitalized patients in the democratic republic of congo in 2003. to investigate their overall genomic relatedness and to determine to which genogroup they belonged, the complete genomes of strains drc88 (g8p[8]) and drc86 (g8p[6]) were determined. genomic comparison of these two african g8 strains revealed that 10 out of their 11 gene segments, except for vp4, were nearly identical (>98.9% identical at the nucleotide level), sugges ... | 2006 | 16672410 |
| changing pattern of human group a rotaviruses: emergence of g12 as an important pathogen among children in eastern india. | rotavirus genotypes, g1-g4 and g9 are associated with childhood diarrhoea throughout the world. in our previous study, we detected g1, g2, g4 and three g12 strains from kolkata, india. | 2006 | 16679056 |
| outbreak of sapovirus infection among infants and children with acute gastroenteritis in osaka city, japan during 2004-2005. | one hundred and twenty five fecal specimens were collected from sporadic cases of acute gastroenteritis in a pediatric clinic in osaka city, japan from july 2004 to june 2005 and tested for the presence of rotavirus, norovirus, sapovirus, astrovirus, and adenovirus by rt-multiplex pcr. among diarrheal viruses detected, norovirus was the most prevalent (19.2%, 24 of 125), followed by group a rotavirus (18.4%, 23 of 125), astrovirus (1.6%, 2 of 125), and adenovirus (0.8%, 1 of 125), respectively. ... | 2006 | 16628578 |
| genetic characterization of vp3 gene of group a rotaviruses. | we sequenced vp3 gene of four rotavirus strains and examined the diversity of vp3 and its other genetic characteristics in a total of 22 group a rotaviruses. the 22 rotavirus strains were derived from six different host species (human, avian, bovine, equine, simian and porcine). comparison of the partial vp3 peptide sequences (aa 141-294) showed identities ranging from 49 to 99% among different species. the phylogenetic analysis of vp3 sequences revealed segregation according to the species orig ... | 2006 | 16972027 |
| comparative molecular characterization of gene segment 11-derived nsp6 from lamb rotavirus llr strain used as a human vaccine in china. | sequence-length polymorphism is known for rotavirus genetic segment 11 (encodes non-structural protein, nsp6). with the exception of 11 strains that have the coding potential for a 98-residue nsp6, majority of the strains have the potential for a 92-residue nsp6. in nine strains, the coding potential for this protein is even shorter. this report focuses on the nsp6 gene nucleotide sequence of lanzhou lamb rotavirus (llr) strain and its comparative molecular characterization. the llr strain is a ... | 2006 | 16492399 |
| expression and purification of rotavirus proteins nsp5 and nsp6 in escherichia coli. | rotaviruses are one of the worldwide leading causes of gastroenteritis in children under 5 yr old. the rotavirus nonstructural nsp5 is a phosphoprotein implicated in viroplasms formation, whereas nsp6 could have a possible regulatory role of nsp5. it has been reported that n- and c-termini of nsp5 are important for their function. however, no structural information on nsp5 and nsp6 proteins is available. because a high amount of protein is required for structural analysis, efficient expression s ... | 2006 | 16679520 |
| isolation and characterization of a new simian rotavirus, yk-1. | to effectively analyze the requirements for protection to rotavirus infection, a reliable animal model that reasonably mimics infection and disease in humans is needed. a requirement for an effective animal model is the availability of appropriate rotavirus stocks for challenge. | 2006 | 16737519 |
| molecular epidemiology of group a rotavirus diarrhea among children in buenos aires, argentina, from 1999 to 2003 and emergence of the infrequent genotype g12. | to examine the epidemiology of rotaviruses in buenos aires, argentina, we screened 1,212 stool samples from children with diarrhea in the southern district of buenos aires from 1999 to 2003. we identified 187 samples (15.4%) that were positive for group a rotavirus by use of antigen enzyme-linked immunosorbent assay. among these specimens, 112 were available for typing: 93 (83.0%) were single-type infections, 9 (8.0%) were mixed-type infections with more than one g or p type, and 10 (8.9%) were ... | 2006 | 16757596 |
| rt-pcr based diagnosis revealed importance of human group b rotavirus infection in childhood diarrhoea. | human group b rotavirus was first identified as causative agent of a large outbreak of severe gastroenteritis affecting more than 1 million people, predominantly adults in china in 1982-1983. in spite of serological evidences for the presence of group b rotavirus in many countries of the world, the virus has been detected only from china, india and bangladesh, where most of the cases were from adults. | 2006 | 16765641 |
| frequency of group a rotavirus in diarrhoeic calves in brazilian cattle herds, 1998-2002. | the frequency of group a bovine rotavirus (gpa brv) in calves from 1998 to 2002 was determined by the polyacrylamide gel electrophoresis technique in 2177 faecal samples, of which 1898 samples were diarrhoeic and 279 were of normal consistency (control group) that were collected from asymptomatic calves for comparative purposes. the animals were from beef and dairy cattle herds (n = 321) from 158 counties in seven states (paraná, são paulo, minas gerais, mato grosso do sul, mato grosso, goiás an ... | 2006 | 17265766 |
| multiplex reverse transcription-pcr for rapid differential detection of porcine epidemic diarrhea virus, transmissible gastroenteritis virus, and porcine group a rotavirus. | a novel multiplex reverse transcription polymerase chain reaction (multiplex rt-pcr) that can detect porcine epidemic diarrhea virus (pedv), transmissible gastroenteritis virus (tgev), and porcine group a rotavirus (gar) was developed. the 3 viruses (pedv, tgev, and porcine gar) are major agents in viral enteric diseases of piglets. as the clinical signs of these diseases are similar, including watery diarrhea, differential detection is required for etiologic diagnosis. a mixture of 3 pairs of p ... | 2006 | 16789718 |
| rotavirus genome replication and morphogenesis: role of the viroplasm. | the rotaviruses, members of the family reoviridae, are icosahedral triple-layered viruses with genomes consisting of 11 segments of double-stranded (ds)rna. a characteristic feature of rotavirus-infected cells is the formation of large cytoplasmic inclusion bodies, termed viroplasms. these dynamic and highly organized structures serve as viral factories that direct the packaging and replication of the viral genome into early capsid assembly intermediates. migration of the intermediates to the en ... | 2006 | 16909900 |
| viral gastroenteritis in children hospitalised in sicily, italy. | the aim of the present study was to describe the epidemiologic and clinical characteristics of acute viral gastroenteritis in hospitalised italian children. a total of 215 stool specimens were collected from january to december 2003 from patients hospitalised in palermo for acute diarrhoea. samples were tested for group a rotavirus, astrovirus, adenovirus, norovirus, enteropathogenic bacteria, and parasites. rotaviruses, mostly belonging to types g1-g4, were detected in 25.1% of samples, astrovi ... | 2006 | 16917775 |
| molecular epidemiology of group a rotaviruses in water sources and selected raw vegetables in southern africa. | group a rotaviruses (rvs) are the most important cause of acute viral gastroenteritis in infants and young children. in this study raw and treated drinking water supplies at plants in two geographic areas, as well as selected irrigation water and corresponding raw vegetables in three regions of southern africa, were screened for the presence of rvs using molecular techniques. group a rvs were detected in 11.8% of partially treated and 1.7% of finally treated drinking water samples and in 14% of ... | 2006 | 16820443 |
| g-serotypes of group a rotaviruses in pilsen region (czechia). | a total of 260 feces samples from children with confirmed rotavirus infection collected during 1999-2002 were serotyped, using enzymoimmunoassay with vp7 specific monoclonal antibodies for g1-g4 serotypes. the serotypes were identified in 185 feces, i.e. 71.2 %. individual serotypes occurred in 43, 2, 16 and 2 %; 8 % samples reacted with 2 type-specific monoclonal antibodies. the g1 serotype was prevalent over the whole period. the g3 type occurred with a statistically higher significance in chi ... | 2006 | 16821723 |
| molecular characterization of novel g5 bovine rotavirus strains. | group a rotaviruses are a major cause of acute gastroenteritis in young children as well as many domestic animals. the rotavirus genome is composed of 11 segments of double-stranded rna and can undergo genetic reassortment during mixed infections, leading to progeny viruses with novel or atypical phenotypes. the aim of this study was to determine if the bovine group a rotavirus strains kj44 and kj75, isolated from clinically infected calves, share genetic features with viruses obtained from hete ... | 2006 | 16928963 |
| rotavirus diarrhea in children and adults in a southern city of brazil in 2003: distribution of g/p types and finding of a rare g12 strain. | between may and august in 2003, a total of 251 fecal samples were collected from children and adults with diarrhea (5 inpatients and 246 outpatients) at a private hospital in the city of ponta grossa, the state of paraná, brazil. group a rotavirus was detected in 71 of 251 (28.3%) specimens: 55 (77.5%) from children under 5 years of age and 16 (22.5%) from individuals aged 6-72 years. all 71 strains exhibited a "long" rna pattern when analyzed by page. sixty-one positive samples that yielded eno ... | 2006 | 16847962 |
| detection and molecular characterization of group a rotavirus from hospitalized children in rio de janeiro, brazil, 2004. | rotavirus is a major cause of infantile acute diarrhea, causing about 440,000 deaths per year, mainly in developing countries. the world health organization has been recommending the assessment of rotavirus burden and strain characterization as part of the strategies of immunization programs against this pathogen. in this context, a prospective study was made on a sample of 134 children with acute diarrhea and severe dehydration admitted to venous fluid therapy in two state hospitals in rio de j ... | 2006 | 16862325 |
| real-time reverse transcription-pcr for detection of rotavirus and adenovirus as causative agents of acute viral gastroenteritis in children. | viral pathogens are the most common cause of gastroenteritis in developed countries. human rotavirus and adenovirus infections are major causes of acute outbreaks and sporadic cases of gastroenteritis, occurring primarily among children less than 2 years of age. patient hospitalization is often required, with enormous infection control implications. this work describes the development of real-time pcr assays for the detection of group f adenovirus, rotavirus a, and rotavirus c from stool specime ... | 2006 | 16954246 |
| assessment of the quantitative real-time polymerase chain reaction using a cdna standard for human group a rotavirus. | nucleic acid amplification techniques are used frequently for rapid diagnosis of viral diseases. in this study, a real-time polymerase chain reaction protocol that uses primers specific for the viral vp4 gene and the commercial sybr green reagent were evaluated for the quantitative measurement of human rotavirus (hrv) rna in human stool specimens. sybr green i detection involved analysis of the melting temperature of the pcr product and measurement of fluorescence at the optimum temperature. the ... | 2006 | 16890998 |
| detection of rare g3p[19] porcine rotavirus strains in chiang mai, thailand, provides evidence for origin of the vp4 genes of mc323 and mc345 human rotaviruses. | among 175 fecal specimens collected from diarrheic piglets during a surveillance of porcine rotavirus (porv) strains in chiang mai, thailand, 39 (22.3%) were positive for group a rotaviruses. of these, 33.3% (13 of 39) belonged to g3p[19], which was a rare p genotype seldom reported. interestingly, their vp4 nucleotide sequences were most closely related to human p[19] strains (mc323 and mc345) isolated in 1989 from the same geographical area where these porv strains were isolated. these p[19] p ... | 2006 | 16988014 |
| molecular characterisation of the rotavirus strains prevalent in maua, meru north, kenya. | rotavirus is the most common cause of severe infantile diarrhoea disease in infants and young children below five years worldwide. rotavirus is associated with high cases of morbidity and mortality and it is estimated that up to 650,000 deaths in young children occur annually in the less developed countries and approximately 150,000-200,000 deaths occur in africa alone. | 2006 | 17089495 |
| virus diversity of acute diarrhea in tropical highlands. | infectious acute diarrhea (iad) is an important health problem affecting a large number of latin-american children. several reports show that bacteria, parasites and virus are involved in the burden of this disease. most reports reveal rotavirus a as the responsible etiological agent, at the same time, there seems to be some correlation between iad and seasonal weather changes. to learn about the type of microbial agents associated with iad in children during mildly changing yearly climatic cond ... | 2006 | 17357570 |
| molecular characterisation of equine group a rotavirus, nasuno, isolated in tochigi prefecture, japan. | in this study, equine group a rotavirus (rv-a), nasuno, isolated from foal diarrhoea in tochigi prefecture, japan was characterised genetically by sequence analysis of the genome segments encoding vp4 and vp7. the nucleotide and deduced amino acid sequences revealed high homology with p[12] rv-as (94.0-99.3% and 94.9-99.4%) and g3 rv-as (86.9-99.5% and 91.1-99.4%). nasuno was also classified into p[12] and g3 in the phylogenetic analysis of the nucleotide sequences of the genome segments encodin ... | 2006 | 16019242 |
| identification of a novel vp4 genotype carried by a serotype g5 porcine rotavirus strain. | rotavirus genome segment 4, encoding the spike outer capsid vp4 protein, of a porcine rotavirus (porv) strain, 134/04-15, identified in italy was sequenced, and the predicted amino acid (aa) sequence was compared to those of all known vp4 (p) genotypes. the aa sequence of the full-length vp4 protein of the porv strain 134/04-15 showed aa identity values ranging from 59.7% (bovine strain kk3, p8[11]) to 86.09% (porcine strain a46, p[13]) with those of the remaining 25 p genotypes. moreover, aa se ... | 2006 | 16364388 |
| a large multi-pathogen waterborne community outbreak linked to faecal contamination of a groundwater system, france, 2000. | a large waterborne outbreak of infection that occurred during august 2000 in a local community in france was investigated initially via a rapid survey of visits to local physicians. a retrospective cohort study was then conducted on a random cluster sample of residents. of 709 residents interviewed, 202 (28.5%) were definite cases (at least three liquid stools/day or vomiting) and 62 (8.7%) were probable cases (less than three liquid stools/day or abdominal pain). those who had drunk tap water h ... | 2006 | 16700706 |
| human rotavirus serotype g9, são paulo, brazil, 1996-2003. | a total of 3,101 fecal specimens were collected during an 8-year survey for rotavirus infection in são paulo, brazil. group a rotavirus was detected in 774 (25.0%) specimens. of these, 431 strains (55.7%) were analyzed for g and p types by reverse transcription-polymerase chain reaction; g1 was the predominant serotype (68.2%), followed by g9 (17.2%), g4 (6.3%), g2 (1.2%), g3 (0.7%), mixed infection (1.8%), and untypeable (4.6%). both rotavirus g and p types could be established in 332 strains ( ... | 2006 | 16707053 |
| changing distribution of group a rotavirus g-types and genetic analysis of g9 circulating in japan. | a total of 1,797 fecal specimens from infants and children with acute gastroenteritis in japan from july 2000 to june 2003 were tested for group a rotavirus by elisa, rt-pcr, rna-page and latex agglutination methods. of these, 439 were found to be positive for group a rotavirus and this presented 24.4%. in 2000-2001, g1 was the most prevalent (45.5%) followed by g2 (32.5%), g3 (12.3%), g9 (5.9%) and g4 (2.6%). however, g2 was found predominant with 40% in the following year (2001-2002). interest ... | 2006 | 16086098 |
| comparison of nsp4 protein between group a and b human rotaviruses: detection of novel diarrhea-causing sequences in group b nsp4. | the human group b rotavirus is a causative agent of severe adult diarrhea. in this study, we analyzed the nsp4 structure of a group b rotavirus strain, cal-1, and determined whether enterotoxin activity was present in cal-1 nsp4. cal-1 nsp4 was comprised of 219 amino acids which was longer than group a and c rotavirus nsp4, and the primary structures of their sequences differed considerably. however, cal-1 nsp4 had an enterotoxin-like sequence (residues 106-127) that was only 27% identical to th ... | 2006 | 16132179 |
| association of rotavirus viroplasms with microtubules through nsp2 and nsp5. | rotavirus replication and virus assembly take place in electrodense spherical structures known as viroplasms whose main components are the viral proteins nsp2 and nsp5. the viroplasms are produced since early times after infection and seem to grow by stepwise addition of viral proteins and by fusion, however, the mechanism of viropiasms formation is unknown. in this study we found that the viroplasms surface colocalized with microtubules, and seem to be caged by a microtubule network. moreover i ... | 2006 | 17072471 |
| viral gastroenteritis and genetic characterization of recombinant norovirus circulating in eastern russia. | from november 2003 to march 2004 a total of 100 fecal specimens from infants and children with acute gastroenteritis in the city of birobidzhan, eastern russia were tested for the presence of diarrheal viruses by rt-multiplex pcr. of these, 74 fecal specimens were positive for diarrheal viruses and this represented 74%. among the diarrheal viruses detected, group a rotavirus was the most prevalent (67%; 67 of 100), followed by norovirus (4%; 4 of 100), group c rotavirus (1%, 1 of 100), sapovirus ... | 2006 | 16812951 |
| structure-function analysis of rotavirus nsp2 octamer by using a novel complementation system. | viral inclusion bodies, or viroplasms, that form in rotavirus-infected cells direct replication and packaging of the segmented double-stranded rna (dsrna) genome. nsp2, one of two rotavirus proteins needed for viroplasm assembly, possesses ntpase, rna-binding, and helix-unwinding activities. nsp2 of the rotavirus group causing endemic infantile diarrhea (group a) was shown to self-assemble into large doughnut-shaped octamers with circumferential grooves and deep clefts containing nucleotide-bind ... | 2006 | 16873255 |
| detection and identification of echovirus 7 from a child with gastro-enteritis. | gastro-enteritis continues to be a significant cause of mortality in infants and young children in developing countries. some previous studies have associated echoviruses with gastroenteritis. | 2006 | 17685211 |
| rare group a rotavirus p[9]g3 isolated in nara prefecture, japan. | 2006 | 16632920 | |
| an outbreak of rotavirus infection among adults in an institution for rehabilitation: long-term residence in a closed community as a risk factor for rotavirus illness. | an outbreak of group a rotavirus infection resulted in gastroenteritis among disabled adults in an isolated rehabilitation institution in kobe, japan. of the 95 residents, 16 were diagnosed with rotavirus illness. the causative agent was a single strain of typical human group a rotavirus belonging to vp7 serotype g2, vp4 genotype p[4], and nsp4 genotype a. mean duration of stay was significantly longer for residents with rotavirus illness (22.1+/-11.8 years) than for residents without the diseas ... | 2006 | 16798700 |
| [comparison of clinical characteristics of patients with rotavirus gastroenteritis relative to the infecting rotavirus g-p genotype.]. | group a rotavirus is a major cause of severe diarrhea in children throughout the world. for the proper management of rotavirus infections, it will be helpful to know their clinical characteristics according to the g and p genotypes of the infecting virus. | 2006 | 18156706 |
| prevalence of enteropathogenic viruses and molecular characterization of group a rotavirus among children with diarrhea in dar es salaam tanzania. | different groups of viruses have been shown to be responsible for acute diarrhea among children during their first few years of life. epidemiological knowledge of viral agents is critical for the development of effective preventive measures, including vaccines. | 2007 | 18162127 |
| expression and characterization of vp2 protein of human rotavirus a in a mammalian lung cell line. | a system for the expression and characterization of vp2 protein of human rotavirus a, strain g3 was established in the mammalian lung cell line (a549). expression of the recombinant vp2 (rvp2) protein was detected 48-72 hrs after transfection by western blotting. the rvp2 protein expressed in a549 cells formed intracellular core-like particles (clps) 30 nm in diameter detected by electron microscopy (em). these results showed that the a549 cells are suitable as efficient eukaryotic host for prod ... | 2007 | 18197733 |
| detection by pcr of eight groups of enteric pathogens in 4,627 faecal samples: re-examination of the english case-control infectious intestinal disease study (1993-1996). | the english case-control infectious intestinal disease study (1993-1996) failed to detect an enteric pathogen or toxin in 49% of cases of gastroenteritis. in the present study, polymerase chain reaction (pcr) assays were applied to dna and cdna generated from 4,627 faecal samples from cases and controls archived during the original study for the detection of norovirus, rotavirus, sapovirus, campylobacter spp., salmonella spp., enteroaggregative escherichia coli, cryptosporidium spp., and giardia ... | 2007 | 17447091 |
| [rotavirus a among hospitalized infants, up to three years of age, with acute gastroenteritis in campo grande, state of mato grosso do sul]. | polyacrylamide gel electrophoresis and combined immunoenzyme assay for rotavirus and adenovirus were used to analyze 380 fecal samples from children up to three years of age who were hospitalized with acute diarrhea in campo grande, state of mato grosso do sul, between may 2000 and january 2004. among all the samples, 88 (23.2%) were positive for rotavirus a. out of these, 81 (92%) had a defined electrophoretic pattern: 77 (87.5%) with a long pattern and four (4.5%) with a short pattern. genotyp ... | 2007 | 17876462 |
| [eurorotanet--european rotavirus strain surveillance network established with hungarian participation]. | group a rotaviruses are the most common cause of severe gastroenteritis worldwide. the incidence and distribution of group a rotavirus sero/genotypes varies between geographical areas during a rotavirus season, and from one season to the next. in addition, cocirculation of genetically diverse multitypic rotaviruses and of intratypic variants in any one place and time is common. assuming widespread use of rotavirus vaccine in the near future, comprehensive surveillance of natural rotavirus infect ... | 2007 | 17947197 |
| rotavirus and adenovirus in rondônia. | acute gastroenteritis is one of the most common diseases in humans worldwide. viral gastroenteritis is a global problem in infants and young children. in this study the incidence of diarrhea was assessed in 877 hospitalized children under five years old, over a period of 24 months and distributed in 470 cases of diarrhea and 407 age-matched group with other pathologies, as control group. two antigen detection techniques based on enzyme immunoassay (eia) and latex particles were used for detectio ... | 2007 | 17710298 |
| emergence of human rotavirus group a genotype g9 strains, wuhan, china. | of 322 stool specimens collected from children with diarrhea from october 2005 through september 2006 in wuhan, china, group a rotavirus was identified in 101 (31.4%). the most prevalent group a rotavirus genotype was g3p[8] (62.6%), followed by g1p[8](17.6%), g1+g3p[8](8.8%), g3p[4](6.6%), g1p[4](2.2%), and g9p[8](2.2%). the g9 strains were first detected in wuhan. | 2007 | 18258015 |
| [g[p] type profiles of group a human rotavirus and their distribution in nizhni novgorod and dzerzhinsk in 1997-2005]. | rt-pcr was used to determine g (vp7) and p (vp4) genotypes of group a rotavirus found in 2454 children with diarrhea in 1997-2005. eight g[p] combinations, including g1-4 serotypes and p[4, 6, 8, 9] genotypes, were identified. the p[8] genotype was presented by the subtypes p[8]-1 and p[8]-2. in 1997-2005, the spectrum and distribution of g[p] types were as follows: g1p[8]-1 (10%), g1p[8]-2 (67%), g1p[6] (3%), g2p[4] (9%), g3p[8]-2 (5%), g3p[6] (1%), g3p[9] (1%), g4p[8]-2 (3%), g9 (0%), g?p[?] ( ... | 2007 | 17601046 |
| the formation of viroplasm-like structures by the rotavirus nsp5 protein is calcium regulated and directed by a c-terminal helical domain. | the rotavirus nsp5 protein directs the formation of viroplasm-like structures (vls) and is required for viroplasm formation within infected cells. in this report, we have defined signals within the c-terminal 21 amino acids of nsp5 that are required for vls formation and that direct the insolubility and hyperphosphorylation of nsp5. deleting c-terminal residues of nsp5 dramatically increased the solubility of n-terminally tagged nsp5 and prevented nsp5 hyperphosphorylation. computer modeling and ... | 2007 | 17699573 |
| detection of the rotavirus a genome from the cerebrospinal fluid of a gastroenteritis patient: a case report. | 2007 | 17515656 | |
| group a rotavirus strains circulating in the eastern center of tunisia during a ten-year period (1995-2004). | an epidemiological survey investigating rotavirus infections in children was undertaken in the eastern center of tunisia between january 1995 and december 2004. a total of 982 faecal specimens collected from children less than 5 years in age were screened by enzyme-linked immunosorbent assay (elisa) or latex agglutination assay for the presence of group a rotavirus antigen. rotavirus-positive samples were used for g and p typing by multiplex semi-nested reverse transcription-pcr. rotaviruses wer ... | 2007 | 17516524 |
| diversity of group a rotavirus strains circulating in paraguay from 2002 to 2005: detection of an atypical g1 in south america. | group a rotaviruses are the main cause of severe gastroenteritis in children worldwide. | 2007 | 17720620 |
| characterization of clinical and immune response in a rotavirus diarrhea model in suckling lewis rats. | group a rotaviruses (rvs) are the leading pathogens causing diarrhea in children and animals. the present study was designed to establish an experimental model of rv infection and immune response in suckling rats. wistar (w) and lewis (l) suckling rats were inoculated orally with two different doses of a simian rv sa-11 strain. rv infection was evaluated by growth rate and clinical indexes. virus-shedding and serum anti-rv antibodies were measured by enzyme-linked immunosorbent assay (elisa). mu ... | 2007 | 17957154 |
| effects of different animal waste treatment technologies on detection and viability of porcine enteric viruses. | enteric pathogens in animal waste that is not properly processed can contaminate the environment and food. the persistence of pathogens in animal waste depends upon the waste treatment technology, but little is known about persistence of porcine viruses. our objectives were to characterize the porcine enteric viruses (porcine noroviruses [ponovs], porcine sapoviruses [posavs], rotavirus a [rv-a], rv-b, and rv-c) in fresh feces or manure and to evaluate the effects of different candidate environm ... | 2007 | 17601821 |
| group a rotavirus-associated diarrhea in children seeking treatment in indonesia. | globally, group a rotavirus causes significant morbidity and mortality among children. limited data exist on the epidemiology of rotavirus disease among indonesian children. | 2007 | 17977785 |
| an outbreak of adenovirus serotype 41 infection in infants and children with acute gastroenteritis in maizuru city, japan. | a total of 337 fecal specimens were collected from infants and children with acute gastroenteritis in maizuru city, japan from july 2004 to june 2005 and tested for the presence of rotavirus, norovirus, sapovirus, astrovirus, and adenovirus by rt-multiplex pcr. among diarrheal viruses detected, norovirus was the most prevalent (13.6%, 46 of 337), followed by adenovirus (8%, 27 of 337), group a rotavirus (5%, 17 of 337), astrovirus (1.8%, 6 of 337), and sapovirus (1.8%, 6 of 337), respectively. a ... | 2007 | 17157081 |
| molecular epidemiologic analysis of group a rotaviruses in adults and children with diarrhea in wuhan city, china, 2000-2006. | to compare epidemiologic features and genetic characteristics of group a rotaviruses causing diarrhea in children and adults, a survey was conducted in wuhan, china, during the period of dec. 2000-may 2006. a total of 3839 stool specimens from diarrheal patients from eight hospitals were analyzed. winter seasonality was observed for rotavirus diarrhea in both adults and children, showing overall rotavirus-positive rates of 9.0 and 23.9%, respectively. throughout the study period, g3 was the most ... | 2007 | 17195953 |
| anticipating rotavirus vaccines in brazil: detection and molecular characterization of emerging rotavirus serotypes g8 and g9 among children with diarrhoea in recife, brazil. | in 2006, brazil will initiate universal immunization of its 4-million infants with a live attenuated serotype g1p[8] human rotavirus vaccine. in anticipation of the national immunization program, this study was undertaken to characterize rotavirus strains circulating among children in recife, one of the largest cities in the northeast region of brazil. group a rotaviruses were detected in 102 (35%) of 290 faecal specimens collected from children under 5 years of age who presented with acute diar ... | 2007 | 17245712 |
| detection and genetic characterization of group a rotavirus strains circulating among children with acute gastroenteritis in japan. | a total of 603 fecal specimens collected from july 2005 to june 2006 from children with acute gastroenteritis, encompassing five different localities in japan, were screened for group a rotavirus by reverse transcription-pcr. it was found that 117 fecal specimens (19.4%) were positive for group a rotavirus. rotavirus infection was detected continuously from november to june, with the highest prevalence in april. the g (vp7 genotypes) and p (vp4 genotypes) types were further investigated. the pre ... | 2007 | 17301134 |
| diversity of viruses associated with acute gastroenteritis in children hospitalized with diarrhea in ho chi minh city, vietnam. | a molecular epidemiological study on common diarrheal viruses was conducted in ho chi minh city, vietnam between october 2002 and september 2003. fecal samples were collected from 1,010 hospitalized children with acute gastroenteritis. those samples were screened for groups a, b, and c rotavirus, adenovirus, genogroups i and ii norovirus (nov), sapovirus (sav), and human astrovirus (hastv) by rt-multiplex pcr, and the positive specimens were characterized further by elisa, nested pcr, or sequenc ... | 2007 | 17385670 |
| prevalence of group c rotaviruses in weaning and post-weaning pigs with enteritis. | diarrheic fecal specimens collected from porcine herds were screened for the presence of group c rotaviruses using a reverse transcription-polymerase chain reaction (rt-pcr) assay. a total of 188 samples were tested and 54 were positive. when compiled these data with diagnostic results on group a rotaviruses and enteric caliciviruses we found that all but 5 group c rotavirus positive samples contained at least one additional virus. a subset of samples were subjected to nucleotide sequencing. the ... | 2007 | 17416471 |
| group a rotavirus genotypes in germany during 2005/2006. | during the 2005/2006 winter season a total of 802 group a positive rotavirus specimens of patients from different regions throughout germany were genotyped. amplicons from a one-tube rt-pcr were typed by analysis of their (type-specific) size using type-specific primers, fluorescent consensus primers and a capillary sequencer for detection. while g1p[8] was predominant (45.8%), g9p[8] has emerged as the second most frequent genotype combination (37.7%). the distribution of genotypes was heteroge ... | 2007 | 17557132 |
| development and validation of dna microarray for genotyping group a rotavirus vp4 (p[4], p[6], p[8], p[9], and p[14]) and vp7 (g1 to g6, g8 to g10, and g12) genes. | previously, we reported the development of a microarray-based method for the identification of five clinically relevant g genotypes (g1 to g4 and g9) (v. chizhikov et al., j. clin. microbiol. 40:2398-2407, 2002). the expanded version of the rotavirus microarray assay presented herein is capable of identifying (i) five clinically relevant human rotavirus vp4 genotypes (p[4], p[6], p[8], p[9], and p[14]) and (ii) five additional human rotavirus vp7 genotypes (g5, g6, g8, g10, and g12) on one chip. ... | 2007 | 17567783 |
| serotype 1 and 2 bovine noroviruses are endemic in cattle in the united kingdom and germany. | the genomically and antigenically distinct bovine noroviruses bo/jena/1980/de and bo/newbury2/1976/uk have been associated with calf diarrhea. in the present seroprevalence study, both were found to be endemic in cattle from germany and the united kingdom, a finding in contrast to previous virus prevalence studies. they were less common than group a rotaviruses, particularly in calves, suggesting a different epidemiology. | 2007 | 17596356 |
| coupling of rotavirus genome replication and capsid assembly. | the reoviridae family represents a diverse collection of viruses with segmented double-stranded (ds)rna genomes, including some that are significant causes of disease in humans, livestock, and plants. the genome segments of these viruses are never detected free in the infected cell but are transcribed and replicated within viral cores by rna-dependent rna polymerase (rdrp). insight into the replication mechanism has been provided from studies on rotavirus, a member of the reoviridae whose rdrp c ... | 2007 | 17222694 |
| [characteristic of the etiologic structure of acute enteric infections in adults based on the data from infectious diseases hospital]. | study of the etiologic spectrum of sporadic cases of causative agents of acute enteric infections (aei) in 753 patients aged 15-85 years old using polymerase chain reaction (pcr) was performed in the infectious diseases hospital during 12 months. it was shown that detection of the causative agents of aei by pcr was 2.2 times more effective than routine microbiologic diagnostics supplemented with test of feces on rotavirus by elisa method and allowed to detect etiologic agent in 54.3% of the pati ... | 2007 | 18038539 |
| rotavirus infection in the paraguayan population from 2004 to 2005: high incidence of rotavirus strains with short electropherotype in children and adults. | rotavirus is considered the main viral cause of acute gastroenteritis in children in both developed and developing countries. the aim of the present study was to continue the surveillance of rotavirus in the paraguayan population in anticipation of a rotavirus vaccination in children. | 2007 | 17599029 |
| an atpase activity associated with the rotavirus phosphoprotein nsp5. | interactions between nsp5 and nsp2 drive the formation of viroplasms, sites of genome replication and packaging in rotavirus-infected cells. the serine-threonine-rich nsp5 transitions between hypo- and hyper-phosphorylated isomers during the replication cycle. in this study, we determined that purified recombinant nsp5 has a mg2+-dependent atp-specific triphosphatase activity that generates free adp and pi (vmax of 19.33 fmol of product/min/pmol of enzyme). the atpase activity was correlated wit ... | 2007 | 17825341 |
| molecular characterization of group a rotavirus isolates obtained from hospitalized children in salvador, bahia, brazil. | rotavirus is a major cause of infectious diarrhea in infants and young children. the objective of this study was to characterize the genotypes of human rotavirus found in children hospitalized with acute diarrhea in the pediatric hospital prof. hosannah de oliveira of the ufba in salvador, bahia, brazil, during the years of 1999, 2000 and 2002. fecal samples were analyzed (n=358) by methods eiara and sds-page for detection of rotavirus. positive samples of one or two of these methods (n=168) wer ... | 2007 | 17625724 |
| burden of disease & molecular epidemiology of group a rotavirus infections in india. | rotavirus is the major cause of severe dehydrating diarrhoea in young children worldwide. considerable research has been carried out on rotavirus disease in india. this review collated data from 46 epidemiological studies to determine rotavirus positivity rates and genotypes of infecting rotavirus strains from various settings in india. studies on diarrhoea presenting to hospitals, neonatal rotavirus infections, symptomatic and asymptomatic infections in the community and nosocomial enteric infe ... | 2007 | 17642497 |
| changing pattern of rotavirus g genotype distribution in chiang mai, thailand from 2002 to 2004: decline of g9 and reemergence of g1 and g2. | group a rotaviruses are the most common cause of acute viral diarrhea in humans and animals throughout the world. previous surveillance studies of group a rotaviruses in thailand indicated that the dominant types of rotaviruses were changing from time to time. during 2000 and 2001, the g9 rotavirus emerged as the most prevalent genotype, with an exceptionally high frequency (91.6%) in chiang mai, thailand. in the year 2002-2004, group a rotavirus was detected in 98 out of 263 (37.3%) fecal speci ... | 2007 | 17854032 |
| characterization of the nsp6 protein product of rotavirus gene 11. | the 12kda non-structural protein 6 (nsp6) is the least studied of the rotavirus proteins. in an attempt to further characterize this protein mono-specific antisera was generated using purified protein expressed in e. coli. pulse/chase radio-labeling of virus infected cells was used to show that it is expressed at a steady but low rate throughout the virus replication cycle. in contrast to the other rotavirus non-structural proteins, nsp6 was found to have a high rate of turnover, being completel ... | 2007 | 17658646 |
| molecular characterization of a novel bovine group a rotavirus. | in this study, by partial sequence analysis of the genome segments encoding vp5* and vp7, we characterized a novel bovine group a rotavirus, namely, tak2, that was detected from adult cattle diarrhea in tochigi prefecture, japan. the nucleotide (nt) and deduced amino acid (aa) sequences of the genome segments encoding vp5* and half of the amino terminal portion of vp7 of tak2 revealed a low identity with those of group a rotaviruses carrying previously published p and g type specificities (vp5*: ... | 2007 | 17368763 |
| molecular characterization of rare g3p[9] rotavirus strains isolated from children hospitalized with acute gastroenteritis. | in 2004, an epidemiological survey of human rotavirus infection in chiang mai, thailand detected two uncommon human rotavirus strains (cmh120/04 and cmh134/04) bearing au-1-like g3p[9] genotypes in 1 year old children hospitalized with acute gastroenteritis. the cmh120/04 and cmh134/04 rotavirus strains were characterized by molecular analyses of their vp6, vp7, vp8*, and nsp4 gene segments as well as the determination of rna patterns by polyacrylamide gel electrophoresis (page). analysis of the ... | 2007 | 17457907 |
| evaluation of immunochromatography tests for detection of rotavirus and norovirus among vietnamese children with acute gastroenteritis and the emergence of a novel norovirus gii.4 variant. | a prospective study was conducted to evaluate two immunochromatography (icg) tests for detection of group a rotavirus and norovirus gii, the commercial dipstick 'eiken' rota kit (sa scientific, usa) and the nv ic-1 stick (immuno-probe, japan). polymerase chain reaction (pcr) with specific primer pairs (beg9 and vp7-1', for group a rotavirus; cog2f and g2skr, for norovirus gii) was used as the reference method. the results of icg tests were compared with those of reference method. the sensitivity ... | 2007 | 17496324 |
| molecular analysis of the nsp4 and vp6 genes of rotavirus strains recovered from hospitalized children in rio de janeiro, brazil. | group a rotaviruses are the main cause of acute gastroenteritis in children throughout the world. the two outer capsid proteins, vp4 and vp7, define the p and g genotypes, respectively. rotaviruses with p[8]g1, p[4]g2, p[8]g3 and p[8]g4 genotypes are predominant in infecting humans and the g9 genotype is emerging in most continents as the fifth most common g type worldwide. the inner capsid protein vp6 is responsible for subgroup (sg) specificities, allowing classification of rotaviruses into sg ... | 2007 | 17510274 |