Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| impact of co-infections with enteric pathogens on children suffering from acute diarrhea in southwest china. | acute diarrhea is a global health problem, resulting in high morbidity and mortality in children. it has been suggested that enteric pathogen co-infections play an important role in gastroenteritis, but most research efforts have only focused on a small range of species belonging to a few pathogen groups. this study aimed to assess the impact of co-infections with a broad range of enteric pathogens on children aged below five years who suffer from acute diarrhea in southwest china. | 2016 | 27349521 |
| the first detection and whole genome characterization of the g6p[15] group a rotavirus strain from roe deer. | although rotaviruses have been detected in a variety of host species, there are only limited records of their occurrence in deer, where their role is unknown. in this study, group a rotavirus was identified in roe deer during a study of enteric viruses in game animals. 102 samples of intestinal content were collected from roe deer (56), wild boars (29), chamois (10), red deer (6) and mouflon (1), but only one sample from roe deer was positive. following whole genome sequence analysis, the rotavi ... | 2016 | 27374907 |
| distribution of rotavirus vp7 and vp4 genotypes circulating in tunisia from 2009 to 2014: emergence of the genotype g12. | group a rotavirus (rva) represents the most important aetiological agent of diarrhoea in children worldwide. from january 2009 to december 2014, a multi-centre study realized through 11 tunisian cities was undertaken among children aged <5 years consulting or hospitalized for acute gastroenteritis. a total of 1127 faecal samples were collected. all samples were screened by elisa for the presence of rva antigen. rva-positive samples were further analyzed by page and used for g/p-genotyping by sem ... | 2016 | 27375269 |
| peptide-recombinant vp6 protein based enzyme immunoassay for the detection of group a rotaviruses in multiple host species. | we developed a novel enzyme immunoassay for the detection of group a rotavirus (rva) antigen in fecal samples of multiple host species. the assay is based on the detection of conserved vp6 protein using anti-recombinant vp6 antibodies as capture antibodies and anti-multiple antigenic peptide (identified and constructed from highly immunodominant epitopes within vp6 protein) antibodies as detector antibodies. the clinical utility of the assay was evaluated using a panel of 914 diarrhoeic fecal sa ... | 2016 | 27391106 |
| whole genomic characterization of korean porcine g8p[7] reassortant rotaviruses. | this study analyzed eleven genomic segments of three korean porcine g8p[7] group a rotavirus (rva) strains. phylogenetically, these strains contained two bovine-like and nine porcine-like genomic segments. eight genes (vp1, vp2, vp6 and nsp1-nsp5) of strains 156-1 and 42-1 and seven genes (vp1, vp2, vp6 and nsp2-nsp5) of strain c-1 clustered closely with porcine and porcine-like animal strains and distantly from typical human wa-like strains. the vp3-m2 genotype of these strains clustered closel ... | 2016 | 27393603 |
| [genomic characterization of an unusual human g3p[3] rotavirus with multiple cross-species reassortment]. | one unusual human g3p[3] group a rotavirus (rva) strain m2-102 was identified in stool sample collected from a child with diarrhea in guangxi province, china in 2014. it is well known that g3p[3] is a genotype commonly identified in feline and canine rvas. however, the preliminary phylogenetic analyses of the vp7 and vp4 genes of strain m2-102 indicated that these two genes were closely related to bat rva strain myas33 and simian strain rrv, respectively, whereas both clustered distantly to feli ... | 2016 | 27396154 |
| great genetic diversity of rotaviruses detected in piglets with diarrhea in thailand. | a total of 491 fecal specimens collected from diarrheic piglets in thailand from january 2011 to march 2014 were screened for group a rotavirus by rt-pcr assay. the g and p genotypes of the detected rotaviruses were determined by multiplex pcr or nucleotide sequencing. group a rotaviruses were detected in 113 out of 491 (23.0 %) fecal specimens. a wide variety of g-p genotype combinations were identified, and g4p[13] was the most prevalent genotype combination (29.2 %), followed by g4p[23] (14.1 ... | 2016 | 27412716 |
| sensitive and specific nested pcr assay for detection of rotavirus a in samples with a low viral load. | techniques such as the real-time reverse transcription-polymerase chain reaction (qrt-pcr) can detect rna in samples with a low viral load. however, these amplicons typically are either too short or at insufficient concentrations for use in subsequent sequencing reactions for genotyping and detection confirmation. the assay developed in this study detects rotavirus g genotypes and p genotypes with viral loads as low as 6.2 and 8.2 copies per reaction, respectively. the assay was validated using ... | 2016 | 27421626 |
| therapeutics insight with inclusive immunopharmacology explication of human rotavirus a for the treatment of diarrhea. | rotavirus is the most common cause of severe infant and childhood diarrhea worldwide, and the morbidity and mortality rate is going to be outnumbered in developing countries like bangladesh. to mitigate this substantial burden of disease, new therapeutics such as vaccine and drug are swiftly required against rotavirus. the present therapeutics insight study was performed with comprehensive immunoinformatics and pharmacoinformatics approach. t and b-cell epitopes were assessed in the conserved re ... | 2016 | 27445802 |
| virus reduction during advanced bardenpho and conventional wastewater treatment processes. | the present study investigated wastewater treatment for the removal of 11 different virus types (pepper mild mottle virus; aichi virus; genogroup i, ii, and iv noroviruses; enterovirus; sapovirus; group-a rotavirus; adenovirus; and jc and bk polyomaviruses) by two wastewater treatment facilities utilizing advanced bardenpho technology and compared the results with conventional treatment processes. to our knowledge, this is the first study comparing full-scale treatment processes that all receive ... | 2016 | 27447291 |
| group a rotavirus gastroenteritis: post-vaccine era, genotypes and zoonotic transmission. | abstractthis article provides a review of immunity, diagnosis, and clinical aspects of rotavirus disease. it also informs about the changes in epidemiology of diarrheal disease and genetic diversity of circulating group a rotavirus strains following the introduction of vaccines. group a rotavirus is the major pathogen causing gastroenteritis in animals. its segmented rna genome can lead to the emergence of new or unusual strains in human populations via interspecies transmission and/or reassortm ... | 0 | 27462899 |
| human p[6] rotaviruses from sub-saharan africa and southeast asia are closely related to those of human p[4] and p[8] rotaviruses circulating worldwide. | p[6] rotaviruses have been circulating with a high prevalence in african and, to a more limited extent, asian countries, but they have not been highly prevalent in other parts of the world. | 2016 | 27471320 |
| high incidence of group a rotaviruses g4p[6] strains among children in gyeonggi province of south korea, from 2009 to 2012. | the genotype distribution of group a rotaviruses (rvas) circulating in gyeonggi province, south korea between 2009 and 2012 was investigated. a total of 2619 stool specimens from sporadic acute gastroenteritis cases and 117 acute gastroenteritis outbreaks were analyzed. among them, rvas were detected from 263 (10.0%) sporadic cases and 3 (2.6%) outbreaks. the g4p[6] strains predominated (29.7%), followed by g1p[8] (19.4%), g2p[4] (15.6%), g3p[8] (13.3%) and g9p[8] (6.5%) strain. especially 96.2% ... | 2016 | 27480917 |
| rotavirus epidemiology and surveillance before vaccine introduction in argentina, 2012-2014. | group a rotavirus has been widely described as one of the most important infantile diarrheal pathogens worldwide. in argentina, it is responsible for over 200,000 acute diarrhea cases and from 30 to 50 deaths annually in children under 5 years. the aim of this study is to analyze frequency, seasonality, age group distribution, and circulating genotypes based on data notified in the 2012-2014 period and in turn to assess the pre-vaccine scenario, considering that rotavirus vaccine was introduced ... | 2017 | 27487415 |
| a cross-sectional study on aetiology of diarrhoeal disease, india. | global, regional and national estimates clearly place diarrhoeal diseases as a major, albeit to an extant neglected public health problem. deaths of children aged <5 years owing to diarrhoea was estimated to be 1.87 million at the global level (uncertainty range from 1.56 to 2.19 million), which is approximately 19% of total child deaths. | 2017 | 27514965 |
| group a rotavirus in brazilian bats: description of novel t15 and h15 genotypes. | this study aimed to survey for group a rotaviruses (rva) in bats from brazil and to perform phylogenetic inferences for vp4, vp7, nsp3, nsp4 and nsp5 genes. rva was found in 9.18 % (28/305) of tested samples. the partial genotype constellation of a molossus molossus rva strain was g3-p[3]-ix-rx-cx-mx-ax-nx-t3-e3-h6, and that of a glossophaga soricina rva strain was g20-p[x]-ix-rx-cx-mx-ax-nx-t15-ex-h15. these findings demonstrate an important role of bats in rva epidemiology and provide evidence ... | 2016 | 27518402 |
| epidemiology of group a rotavirus infection after the introduction of monovalent vaccine in the national immunization program of saudi arabia. | this study aimed to investigate the prevalence of group a rotavirus (rva) gastroenteritis and the distribution of the rva genotypes as well as to determine a possible change in the age of occurrence of the rva infection in the first 2 years after rotarix® vaccine introduction in saudi arabia. this descriptive study included 850 hospitalized children <5 years of age with acute gastroenteritis (ag) between october 2013 and september 2015. overall, 78 (9.2%) children were positive for rva during th ... | 2017 | 27531633 |
| prevalence of histopathological intestinal lesions and enteric pathogens in dutch commercial broilers with time. | intestinal disease has a major impact on the broiler industry due to economic and welfare reasons. intestinal disease might occur due to a large number of reasons varying from well-defined pathogens to non-specific enteritis and complex syndromes. however, knowledge about the nature of intestinal disease and presence of enteric viruses in the dutch broiler industry is largely absent. therefore, a large-scale field study, in which 98 broiler flocks from 86 farms were sampled weekly, was started t ... | 2017 | 27557362 |
| predominant prevalence of human rotaviruses with the g1p[8] and g8p[8] genotypes with a short rna profile in 2013 and 2014 in sukhothai and phetchaboon provinces, thailand. | of 2,754 stool specimens collected from children with acute gastroenteritis during 2013-2014 in sukhothai and phetchaboon provinces, thailand, 666 (24.2%) were positive for rotavirus a (rva) in polyacrylamide gel electrophoresis (page). the g and p types of all rva-positive specimens were determined by semi-nested rt-pcr. g1p[8] (56.5%) was most prevalent, followed by g2p[4] (22.1%). unusual g8p[8] human rvas (hurvas) were detected at a high frequency (20.0%). interestingly, 171 of the 376 g1p[8 ... | 2017 | 27557434 |
| genomic constellation and evolution of ghanaian g2p[4] rotavirus strains from a global perspective. | understanding of the genetic diversity and evolution of rotavirus a (rva) strains, a common cause of severe diarrhoea in children, needs to be based on the analysis at the whole genome level in the vaccine era. this study sequenced the whole genomes of six representative g2p[4] strains detected in ghana from 2008 to 2013, and analysed them phylogenetically with a global collection of g2p[4] strains and african non-g2p[4] ds-1-like strains. the genotype constellation of the study strains was g2-p ... | 2016 | 27569866 |
| multi-reassortant g3p[3] group a rotavirus in a horseshoe bat in zambia. | group a rotavirus is a major cause of diarrhoea in humans, especially in young children. bats also harbour group a rotaviruses, but the genetic backgrounds of bat rotavirus strains are usually distinct from those of human rotavirus strains. we identified a new strain of group a rotavirus in the intestinal contents of a horseshoe bat in zambia. whole genome sequencing revealed that the identified virus, named rva/bat-wt/zmb/lus12-14/2012/g3p[3], possessed the genotype constellation g3-p[3]-i3-r2- ... | 2016 | 27574104 |
| epidemiological survey of enteric viruses in wild boars in the czech republic: first evidence of close relationship between wild boar and human rotavirus a strains. | population of wild boar is increasing in the whole europe, the animals migrate close to human habitats which greatly increases the possibility of natural transmission between domestic animals or humans and wild boars. the aim of the study was to estimate in population of free-living wild boar in the czech republic the prevalence of enteric viral pathogens, namely rotavirus groups a and c (rva and rvc), porcine reproductive and respiratory syndrome virus (prrsv), and members of family coronavirid ... | 2016 | 27599927 |
| anti-vp6 vhh: an experimental treatment for rotavirus a-associated disease. | species a rotaviruses (rva) remain a leading cause of mortality in children under 5 years of age. current treatment options are limited. we assessed the efficacy of two vp6-specific llama-derived heavy chain antibody fragments (vhh) -2kd1 and 3b2- as an oral prophylactic and therapeutic treatment against rva-induced diarrhea in a neonatal mouse model inoculated with virulent murine rva (ecw, g16p[16]i7). joint therapeutic administration of 2kd1+3b2 (200 μg/dose) successfully reduced diarrhea dur ... | 2016 | 27603013 |
| molecular epidemiology of rotavirus a, causing acute gastroenteritis hospitalizations among children in nha trang, vietnam, 2007-2008: identification of rare g9p[19] and g10p[14] strains. | rotavirus a (rva) causes acute diarrhea in children as well as animals. as part of a cross-sectional study of children less than 5 years of age hospitalized for acute diarrhea in vietnam during a 15-month period (2007-2008), 322 (43.5%) of 741 fecal specimens contained rva with 92% either g1p[8] or g3p[8]. this study was undertaken to further characterize strains that remained untypeable to complete the g and p genotypes of the 322 rotavirus-positive specimens. while 307 (95.3%) strains possesse ... | 2017 | 27611738 |
| continuous detection and genetic diversity of human rotavirus a in sewage in eastern china, 2013-2014. | rotavirus is the leading viral agent for pediatric gastroenteritis. however, the case-based surveillance for rotavirus is limited in china, and its circulation in the environment is not well investigated. | 2016 | 27623961 |
| cosavirus, salivirus and bufavirus in diarrheal tunisian infants. | three newly discovered viruses have been recently described in diarrheal patients: cosavirus (cosv) and salivirus (salv), two picornaviruses, and bufavirus (buv), a parvovirus. the detection rate and the role of these viruses remain to be established in acute gastroenteritis (age) in diarrheal tunisian infants. from october 2010 through march 2012, stool samples were collected from 203 children <5 years-old suffering from age and attending the children's hospital in monastir, tunisia. all sample ... | 2016 | 27631733 |
| molecular epidemiology of rotavirus gastroenteritis in central kenya before vaccine introduction, 2009-2014. | between july 2009 and june 2014, a total of 1,546 fecal specimens were collected from children <5 years of age with acute gastroenteritis admitted to kiambu county hospital, central kenya. the specimens were screened for group a rotavirus (rva) using elisa, and rva-positive specimens were subjected to semi-nested rt-pcr to determine the g and p genotypes. rva was detected in 429/1,546 (27.5%) fecal specimens. rva infections occurred in all age groups <59 months, with an early peak at 6-17 months ... | 2017 | 27648929 |
| the molecular epidemiology of bovine rotaviruses circulating in iran: a two-year study. | bovine group a rotavirus (bovine rva) is recognized as a major cause of severe gastroenteritis in newborn calves. the purpose of this study was to estimate the prevalence and identify the genotypes of circulating bovine rva in newborn diarrheic calves. two hundred fifty-three stool samples of diarrheic calves up to 1 month old were collected from 42 industrial dairy farms in two iranian provinces during march 2010 to february 2012. all collected samples were screened for the presence of bovine r ... | 2016 | 27654669 |
| whole genome sequencing of a rare rotavirus from archived stool sample demonstrates independent zoonotic origin of human g8p[14] strains in hungary. | genotype p[14] rotaviruses in humans are thought to be zoonotic strains originating from bovine or ovine host species. over the past 30 years only few genotype p[14] strains were identified in hungary totaling<0.1% of all human rotaviruses whose genotype had been determined. in this study we report the genome sequence and phylogenetic analysis of a human genotype g8p[14] strain, rva/human-wt/hun/182-02/2001/g8p[14]. the whole genome constellation (g8-p[14]-i2-r2-c2-m2-a11-n2-t6-e2-h3) of this st ... | 2017 | 27671785 |
| whole-genome characterisation of g12p[6] rotavirus strains possessing two distinct genotype constellations co-circulating in blantyre, malawi, 2008. | rotavirus a strains detected in diarrhoeal children commonly possess any one of the genotypes g1, g2, g3, g4, and g9, with a recent increase in g12 detection globally. g12p[6] strains possessing short rna (ds-1-like) and long rna (wa-like) migration patterns accounted for 27 % of the strains circulating in blantyre, malawi, between 2007 and 2008. to understand how the g12p[6] strains with two distinct genetic backgrounds emerged in malawi, we conducted whole-genome analysis of two long-rna and t ... | 2017 | 27718073 |
| the prevalence and genotype distribution of rotavirus a infection among children with acute gastroenteritis in kunming, china. | the aim of this study was to determine the prevalence of rotavirus a (rva) infections in children from kunming, china, and the rva genotypes present. a total of 16,311 children with acute gastroenteritis were recruited for the study, and 33.1 % (5,394/16,311) were rva positive. children under 24 months of age were more susceptible to rva infection, with an infection rate of 87.4 % (4,712/5,394). the most prevalent genotype was g9p[8] (85/107, 79.4 %), which showed high sequence similarity to g9p ... | 2017 | 27718075 |
| whole genomic analysis of g2p[4] human rotaviruses in mymensingh, north-central bangladesh. | rotavirus a (rva) is a dominant causative agent of acute gastroenteritis in children worldwide. g2p[4] is one of the most common genotypes among human rotavirus (hrv) strains, and has been persistently prevalent in south asia including bangladesh. in the present study, whole genome sequences of a total of 16 g2p[4] hrv strains (8 strains each in 2010 and 2013) detected in mymensingh, north-central bangladesh were determined. these strains had typical ds-1-like genotype constellation. most of gen ... | 2016 | 27722206 |
| multiplex rt-pcr for rapid detection of viruses commonly causing diarrhea in pediatric patients. | multiplex rt-pcr method using five sets of panel primers was developed for the detection of diarrheal viruses, including rotavirus a, b, and c, adenovirus, astrovirus, norovirus gi and gii, sapovirus, aichi virus, parechovirus, enterovirus, cosavirus, bocavirus, and saffold virus. the sensitivity of the method was evaluated and tested with 751 fecal specimens collected from japanese children with acute diarrhea. several kinds of viruses were detected in 528 out of 751 (70.3%) fecal specimens. mi ... | 2017 | 27735999 |
| [surveillance for viral diarrhea in sentinel hospitals in henan province, 2013-2015]. | objective: to investigate the infection status of human rotavirus, calicivirus, astrovirus and enteric adenovirus in children aged <5 years in disease surveillance areas in henan province from 2013 to 2015. methods: a total of 880 stool samples were collected from four sentinel hospitals and group a rotavirus was detected by elisa and group a rotavirus g/p genotyping was performed with nested multiplex rt-pcr, while rotavirus (group b, c), calicivirus and astrovirus were detected by two-step mul ... | 2016 | 27765132 |
| whole genomic analysis of bovine group a rotavirus strains a5-10 and a5-13 provides evidence for close evolutionary relationship with human rotaviruses. | bovine group a rotavirus (rva) is an important cause of acute diarrhea in calves worldwide. in order to obtain precise information on the origin and evolutionary dynamics of bovine rva strains, we determined and analyzed the complete nucleotide sequences of the whole genomes of six archival bovine rva strains; four thai strains (rva/cow-tc/tha/a5-10/1988/g8p[1], rva/cow-tc/tha/a5-13/1988/g8p[1], rva/cow-tc/tha/61a/1989/g10p[5], and rva/cow-tc/tha/a44/1989/g10p[11]), one american strain (rva/cow- ... | 2016 | 27771069 |
| antiviral activity of ergoferon against group a rotavirus. | antiviral activity of ergoferon was studied in vitro on an experimental model of rotavirus infection in ma-104 cell line. in infected cells treated with ergoferon, rotavirus titer was shown to decrease by 83 and 90% in comparison with cells treated with solvent used for ergoferon preparation (p<0.05) and distilled water (p<0.05), respectively. these findings demonstrate high anti-rotavirus activity of ergoferon. | 2016 | 27783292 |
| predominance of unusual rotavirus g1p[6] strain in north india: an evidence from hospitalized children and adult diarrheal patients. | group a rotavirus remains the leading cause of gastroenteritis in children and accounts for 0.2 million fatalities each year; out of which, approximately 47,100 deaths occur in india. in adults also, rotavirus is reported to be responsible for diarrhea severe enough to require hospitalizations. india has recently introduced rotavirus vaccine in the universal immunization programme and himachal pradesh became the first indian state to implement this project. this study is an attempt to provide th ... | 2016 | 27806915 |
| first molecular detection and vp7 (g) genotyping of group a rotavirus by semi-nested rt-pcr from sewage in nigeria. | rotavirus is the leading cause of viral gastroenteritis worldwide, and sewage is a major source of the virus dissemination in the environment. our aim was to detect and genotype rotaviruses from sewages in nigeria. one hundred and ninety sewage samples were collected between june 2014 and january 2015. the two phase concentration method using peg 6000 and dextran was used to concentrate sewage samples following who protocols. molecular detection was performed by rt-pcr, and vp7 genotyping by sem ... | 2016 | 27828615 |
| detection of a novel circovirus pcv3 in pigs with cardiac and multi-systemic inflammation. | porcine circovirus 2 causes different clinical syndromes resulting in a significant economic loss in the pork industry. three pigs with unexplained cardiac and multi-organ inflammation that tested negative for pcv2 and other known porcine pathogens were further analyzed. | 2016 | 27835942 |
| analysis of a genotype g3p[9] rotavirus a strain that shows evidence of multiple reassortment events between animal and human rotaviruses. | the species a rotaviruses (rva) are important gastroenteric pathogens that infect humans and animals. rva genotype g3p[9] has been described in human-animal reassortment events, and the complexity of its hosts motivates the genetic investigation of this strain. therefore, the aim of this study is to analyse a g3p[9] sample that was detected in a child with acute gastroenteritis. the 1a3739 sample featured the constellation g3p[9]-i18-r3-c3-mx-a19-n3-t3-e3-h6. the sequence for vp3 gene was not ob ... | 2016 | 27862014 |
| evolution of ds-1-like g1p[8] double-gene reassortant rotavirus a strains causing gastroenteritis in children in vietnam in 2012/2013. | rotavirus a (rva) strains, a leading cause of severe gastroenteritis in children worldwide, commonly possess the wa or ds-1 genotype constellations. during a hospital-based study conducted in hanoi, vietnam, in the 2012-2013 rotavirus season, g1p[8] strains with a virtually identical short rna migration pattern were detected in 20 (14%) of 141 rotavirus-positive samples. two representatives of these strains were shown by whole-genome sequencing to be double-gene reassortants possessing the genot ... | 2017 | 27878638 |
| from lab to lake - evaluation of current molecular methods for the detection of infectious enteric viruses in complex water matrices in an urban area. | quantitative pcr methods are commonly used to monitor enteric viruses in the aquatic environment because of their high sensitivity, short reaction times and relatively low operational cost. however, conclusions for public health drawn from results of such molecular techniques are limited due to their inability to determine viral infectivity. ethidium monoazide (ema) and propidium monoazide (pma) are capable to penetrate the damaged or compromised capsid of the inactivated viruses and bind to the ... | 2016 | 27880820 |
| case-control study of diarrheal disease etiology in individuals over 5 years in southwest china. | acute diarrhea is one of the major public health problems worldwide. most of studies on acute diarrhea have been made on infants aged below 5 years and few efforts have been made to identify the etiological agents of acute diarrhea in people over five, especially in china. | 2016 | 27891182 |
| analysis of complete genome sequences of g9p[19] rotavirus strains from human and piglet with diarrhea provides evidence for whole-genome interspecies transmission of nonreassorted porcine rotavirus. | whole genomes of g9p[19] human (rva/human-wt/tha/cmh-s070-13/2013/g9p[19]) and porcine (rva/pig-wt/tha/cmp-015-12/2012/g9p[19]) rotaviruses concurrently detected in the same geographical area in northern thailand were sequenced and analyzed for their genetic relationships using bioinformatic tools. the complete genome sequence of human rotavirus rva/human-wt/tha/cmh-s070-13/2013/g9p[19] was most closely related to those of porcine rotavirus rva/pig-wt/tha/cmp-015-12/2012/g9p[19] and to those of ... | 2017 | 27894992 |
| virus variation resource - improved response to emergent viral outbreaks. | the virus variation resource is a value-added viral sequence data resource hosted by the national center for biotechnology information. the resource is located at http://www.ncbi.nlm.nih.gov/genome/viruses/variation/ and includes modules for seven viral groups: influenza virus, dengue virus, west nile virus, ebolavirus, mers coronavirus, rotavirus a and zika virus each module is supported by pipelines that scan newly released genbank records, annotate genes and proteins and parse sample descript ... | 2016 | 27899678 |
| high genetic diversity of species a rotaviruses detected in swine farms in chile. | rotavirus a is one of the main causative agents of diarrhea in lactating and weaned pigs worldwide. its impact in the swine industry is well documented. however, in chile the current epidemiological status of rotavirus in porcine farms is unknown. this study evaluated the current epidemiologic status of rotavirus a infection in chile using on-farm detection technique, electrophoretic confirmation, genotyping and phylogenetic clustering by analysis of partial sequences of vp4 and vp7 genes. rotav ... | 2016 | 27902360 |
| detection of a novel equine-like g3 rotavirus associated with acute gastroenteritis in brazil. | genotype g3p[8] of rotavirus a (rva) is detected worldwide, usually associated with wa-like constellation and exhibiting a long rna migration pattern. more recently, a novel inter-genogroup, g3p[8] reassortant variant with a short electropherotype, has emerged in asia, oceania and europe, denoting an overall potential of unusual rotavirus strains. during a rva surveillance in brazil, g3p[8] strains were found displaying a short electropherotype pattern, which had not been detected before in this ... | 2016 | 27902376 |
| medicinal plants and natural molecules with in vitro and in vivo activity against rotavirus: a systematic review. | rotaviruses can cause life-threatening health disorders, such as severe dehydrating gastroenteritis and diarrhea in children. vaccination is the main preventive strategy to reduce rotavirus diarrhea and the severity of episodes, but vaccines are not fully effective and new episodes may occur, even in vaccinated children. the who recommends oral rehydration therapy and zinc supplementation for rotavirus-induced diarrhea management. there is little preclinical evidence to support the use of phytot ... | 2016 | 27912886 |
| detection and whole-genome characterization of a g8p[1] group a rotavirus strain from deer. | rotavirus a strain 14-02218-2, with genome constellation g8p[1]-i2-r2-c2-m2-a3-n2-t6-e2-h3, was isolated from newborn fawns. the 14-02218-2 rotavirus strain is related to bovine and bovine-like rotavirus strains. to our knowledge, this is the first report on whole-genome-based characterization of a deer rotavirus g8p[1] strain. | 2016 | 27932644 |
| shedding of porcine circovirus type 1 dna and rotavirus rna by infants vaccinated with rotarix®. | thirty-three infants aged ∼2 months had serial stool samples collected after receipt of rotarix® vaccine dose 1, and were assessed for shedding of porcine circovirus type 1 dna and rotavirus group a rna by molecular methods. we did not find strong evidence that porcine circovirus type 1 replication occurred. porcine circovirus type 1 genome with the same sequence as that in rotarix® was detected in a few infants as late as day ≥ 13; while this timing could suggest there may have been replication ... | 2016 | 27936349 |
| the interaction of rotavirus a pig/china/nmtl/2008/g9p[23] vp6 with cellular beta-actin is required for optimal rv replication and infectivity. | vp6 forms the intermediate layer of the rotavirus (rv) capsid, and it plays important roles after rv penetration and uncoating. these functions rely on its ability to interact with host cell proteins. to gain further insights into the role of vp6 in porcine rv (porv) infection, a glutathione s-transferase pull-down assay was utilized to find unknown cellular factors that interact with vp6. in this study, beta-actin, tropomyosin 1, and 40s ribosomal protein s16 were identified as interaction part ... | 2016 | 27938672 |
| whole-genome characterization of a peruvian alpaca rotavirus isolate expressing a novel vp4 genotype. | the sa44 isolate of rotavirus a (rva) was identified from a neonatal peruvian alpaca presenting with diarrhea, and the full-length genome sequence of the isolate (designated rva/alpaca-tc/per/sa44/2014/g3p[40]) was determined. phylogenetic analyses showed that the isolate possessed the genotype constellation g3-p[40]-i8-r3-c3-m3-a9-n3-t3-e3-h6, which differs considerably from those of rva strains isolated from other species of the order artiodactyla. overall, the genetic constellation of the sa4 ... | 2016 | 27939152 |
| outbreak of g2p[4] rotavirus gastroenteritis in a retirement community, brazil, 2015: an important public health risk? | the present study described a group a rotavirus (rva) outbreak in an age-care facility in brazil, using epidemiologic and molecular diagnostic methods. a descriptive clinical, epidemiological and environmental investigation was conducted. stool samples were collected and screened for rva, norovirus (nov), enteric adenovirus 40/41 (adv 40/41) and astrovirus (astv) using elisa, rt-pcr, qrt-pcr, electron microscopy and sequencing methods. outbreak occurred during 26th-29th october, 2015; 28 individ ... | 2016 | 27964971 |
| molecular detection and characterization of picobirnaviruses in piglets with diarrhea in thailand. | picobirnavirus (pbv) is a small, bi-segmented, double-stranded rna virus. taxonomically, the genus picobirnavirus belongs to the picobirnaviridae family. pbv infects a wide range of hosts and causes opportunistic infections, but its role in diarrheal disease remains unclear. to determine the prevalence and genetic diversity of porcine pbvs in northern thailand, 380 fecal samples collected from diarrheic and non-diarrheic piglets, raised in 22 pig farms, were tested for the presence of pbv. rever ... | 2017 | 28032197 |
| whole genome analysis provides evidence for porcine-to-simian interspecies transmission of rotavirus-a. | we report here whole genome analysis of a porcine rotavirus-a (rva) strain rva/pig-wt/kna/et8b/2015/g5p[13] detected in a diarrheic piglet, and nearly whole genome (except for vp4 gene) analysis of a simian rva strain rva/simian-wt/kna/08979/2015/g5p[x] detected in a non-diarrheic african green monkey (agm) on the island of st. kitts, caribbean region. strain et8b exhibited a g5-p[13]-i5-r1-c1-m1-a8-n1-t7-e1-h1 genotype constellation that was identical to those of brazilian porcine rva g5p[13] s ... | 2017 | 28039076 |
| phylogenetic analysis of partial vp7 gene of the emerging human group a rotavirus g12 strains circulating in tunisia. | group a rotavirus (rva) is the leading cause of severe gastroenteritis in children younger than 5 years. the most common human g-types are g1-4 and g9. g12 genotype is currently emerging worldwide, becoming the sixth most prevalent rva g-genotype. in tunisia, an emergence of g12 rva strains was observed. to understand the evolution and origin of these tunisian g12 strains, phylogenetic analyses were conducted. | 2017 | 28051950 |
| [infection status, clinical symptoms and gene type transition of group a rotavirus in children, less than five years-of-age, with diarrhea in sentinel hospitals of henan province, china]. | objective: to investigate the infectious status, gene type transition and epidemiological features of rotavirus a isolated from infants and children (<59 months-of-age) in sentinel hospitals from 2008 to 2015 in henan province, china. methods: in total, 2 541 stool samples (each 3- 5 ml) were collected from infants and children aged less than five years in two sentinel hospitals and group a rotavirus was detected by a double antibody sandwich elisa. viral rna was extracted from positive samples ... | 2017 | 28056276 |
| [analysis of genetics and phylogenetics of g and p genotype of rotavirus a group in sentinel hospitals of hubei province, 2013-2014]. | 2017 | 28056278 | |
| analysis of porcine rotavirus a enterotoxin gene for structure-function relationship. | rotavirus (rv) infected piglets are presumed to be the latent sources of heterologous rv infection to humans and other animals. in rvs, non-structural protein 4 (nsp4) is the major virulence factor with pleiotropic properties. in this study, we analyzed nsp4 gene from porcine rvs, isolated from diarrheic and non-diarrheic cases, at different levels of protein folding to explore any correlation with their diarrhea inducing capabilities and evolution of nsp4 in porcine population. the full length ... | 2017 | 28057906 |
| diversity in vp3, nsp3, and nsp4 of rotavirus b detected from japanese cattle. | bovine rotavirus b (rvb) is an etiological agent of diarrhea mostly in adult cattle. currently, a few sequences of viral protein (vp)1, 2, 4, 6, and 7 and nonstructural protein (nsp)1, 2, and 5 of bovine rvb are available in the ddbj/embl/genbank databases, and none have been reported for vp3, nsp3, and nsp4. in order to fill this gap in the genetic characterization of bovine rvb strains, we used a metagenomics approach and sequenced and analyzed the complete coding sequences (cds) of vp3, nsp3, ... | 2017 | 28063924 |
| porcine kobuvirus 1 in healthy and diarrheic pigs: genetic detection and characterization of virus and co-infection with rotavirus a. | the porcine kobuvirus 1 (pkv-1) is believed to be an enteric virus. to investigate the prevalence of pkv-1 in pigs, virus was detected by rt-pcr in rectal swabs originating from 414 healthy and diarrheic pigs of different age categories on farms in slovakia. among all ages of animals, pkv-1 was detected equally in diarrheic (63.8%) and clinically healthy (62.9%) pigs. pkv-1 was more often detected in diarrheic (74.6%) than in healthy (64.4%) suckling piglets (<28days) but data was not statistica ... | 2017 | 28087494 |
| distribution of rotavirus vp7 and vp4 genotypes circulating in tunisia from 2009 to 2014: emergence of the genotype g12. | group a rotavirus (rva) represents the most important aetiological agent of diarrhoea in children worldwide. from january 2009 to december 2014, a multi-centre study realized through 11 tunisian cities was undertaken among children aged <5 years consulting or hospitalized for acute gastroenteritis. a total of 1127 faecal samples were collected. all samples were screened by elisa for the presence of rva antigen. rva-positive samples were further analyzed by page and used for g/p-genotyping by sem ... | 2016 | 28118139 |
| zooanthroponotic transmission of rotavirus in haryana state of northern india. | rotaviruses are the major cause of severe gastroenteritis and mortality in young children and animals. due to segmented nature of dsrna genome and wide host range, vast genetic and antigenic diversity exists amongst different isolates of rotaviruses. a total of 230 fecal ovine and caprine samples collected from organized farms and villages in haryana were screened for rotavirus detection. samples were screened by latex agglutination test and rna-page followed by rt-pcr and nucleic acid sequencin ... | 2017 | 28161962 |
| multiplex pcr for the detection of 10 viruses causing encephalitis/encephalopathy and its application to clinical samples collected from japanese children with suspected viral. | acute encephalitis is a serious neurological condition having a high mortality rate and affecting both children and adults. this study aimed to develop a multiplex pcr method for the simultaneous screening of clinical samples for the presence of the 10 viruses presently considered as the major viral causes of acute encephalitis/ encephalopathy in asia. | 2017 | 28164489 |
| enteric viruses and adenovirus diversity in waters from 2016 olympic venues. | rio de janeiro's inner and coastal waters are heavily impacted by human sewage pollution for decades. enteric viruses, including human adenoviruses (hadv), human enterovirus (ev), group a rotavirus (rv) and hepatitis a virus (hav) are more likely to be found in contaminated surface waters. the present work aimed to assess the frequency and loads of ev, hadv-c and -f species, rv and hav in sand and water samples from venues used during the 2016 summer olympics and by tourists attending the event. ... | 2017 | 28185736 |
| full genome analysis of rotavirus g9p[8] strains identified in acute gastroenteritis cases reveals genetic diversity: pune, western india. | group a rotaviruses (rva) are the major enteric etiological agents of severe acute gastroenteritis among children globally. as g9 rva now represents as one of the major human rva genotypes, studies on full genome of this particular genotype are being carried out worldwide. so far, no such studies on g9p[8] rvas have been reported from pune, western part of india. keeping in view of this, the study was undertaken to understand the degree of genetic diversity of the commonly circulating g9p[8] rva ... | 2017 | 28240365 |
| burden of rotavirus and enteric bacterial pathogens among children under five years old hospitalized with diarrhea in suburban and rural areas in kenya. | this study aimed to investigate the incidence of rotavirus and enteric bacterial infections among children up to five years old with diarrhea living in suburban and rural areas in kenya. it was a cross-sectional descriptive study, and a total of 1060 diarrheal fecal specimens were obtained from 722 children at the kiambu county hospital (kch) located in a suburban area and 338 children from the mbita district hospital (mdh) located in a rural part of western kenya, between august 2011 and decemb ... | 2017 | 28250260 |
| letter to the editor: evaluation of an alternative rtth-based real-time reverse transcription-pcr kit for detection of rotavirus a. | a previously published one-step real-time quantitative reverse transcription-polymerase chain reaction (qrt-pcr) targeting the group a rotavirus (rva) nsp3 gene is a sensitive technique for detection of rva (1).…. | 2017 | 28275075 |
| longitudinal study of bovine rotavirus group a in newborn calves from vaccinated and unvaccinated dairy herds. | reports of rotavirus excretion in calves usually result from cross-sectional studies, and in face of the conflicting results regarding protection of calves born to vaccinated dams against diarrhea, the aim of the present study was to evaluate rotavirus excretion in dairy calves born to vaccinated or unvaccinated dams, to identify the genotypes of bovine rotavirus group a (rva) strains isolated from these animals as well as to investigate characteristics of the disease in naturally occurring circ ... | 2017 | 28321789 |
| predominance of g9p[4] rotavirus from children with acute gastroenteritis in northwestern mexico. | in mexico, group a rotavirus (rva) infections remain the most common cause of severe dehydrating diarrhea in children. this study was conducted to examine the circulating rva strains in the northwest region of mexico. rva strains collected from stool samples of children were genotyped, and their partial sequences were analyzed. rt-pcr of the vp4 and vp7 genes showed the partial g9p[4] genotype in all the samples. sequencing and phylogenetic analysis of the partial vp7 gene amplicons of 10 strain ... | 2016 | 28334725 |
| the detection and phylogenetic analysis of porcine deltacoronavirus from guangdong province in southern china. | porcine deltacoronavirus (pdcov) is a newly discovered coronavirus that causes diarrhoea, vomiting and dehydration in sucking and nursing piglets. it was first reported in hong kong in 2012 and has since been discovered in the united states, canada, south korea, mainland china, thailand and laos. pdcov has been experimentally proved to lead to diarrhoea in swine and it was detected positive in pigs in guangdong, southern china. in our study, 252 faecal and intestinal samples from sucking piglets ... | 2017 | 28345292 |
| molecular detection of viruses in kenyan bats and discovery of novel astroviruses, caliciviruses and rotaviruses. | this is the first country-wide surveillance of bat-borne viruses in kenya spanning from 2012-2015 covering sites perceived to have medium to high level bat-human interaction. the objective of this surveillance study was to apply a non-invasive approach using fresh feces to detect viruses circulating within the diverse species of kenyan bats. we screened for both dna and rna viruses; specifically, astroviruses (astvs), adenoviruses (advs), caliciviruses (calvs), coronaviruses (covs), flaviviruses ... | 2017 | 28393313 |
| genotypic and epitope characteristics of group a porcine rotavirus strains circulating in canada. | surveillance of rotavirus a (rva) infections in north america swine populations are limited and not performed over a significant time period to properly assess the diversity of rva strains in swine. the vp7 (g) and vp4 (p) genes of 32 canadian rva strains, circulating between 2009 and 2015 were sequenced, identifying the g3p[13], g5p[7], g9p[7], g9[13], and g9[19] genotype combinations. the canadian rva strains were compared to the rva strains present in the swine prosystems rce rotavirus vaccin ... | 2017 | 28399437 |
| development and validation of monoclonal antibody-based antigen capture elisa for detection of group a porcine rotavirus. | porcine rotavirus-a (porva) is one of the common causes of mild to severe dehydrating diarrhea, leading to losses in weaning and postweaning piglets. a rapid, highly specific, and sensitive antigen-capture enzyme-linked immunosorbent assay (ac-elisa) was developed for detection of porva, by using vp6 (a highly conserved and antigenic protein of group-a rotavirus)-directed rabbit polyclonal antibodies (capture antibody) and murine monoclonal antibodies (detector antibody). the detection limit of ... | 2017 | 28414586 |
| a universal genome sequencing method for rotavirus a from human fecal samples which identifies segment reassortment and multi-genotype mixed infection. | genomic characterization of rotavirus (rov) has not been adopted at large-scale due to the complexity of obtaining sequences for all 11 segments, particularly when feces are used as starting material. | 2017 | 28438140 |
| expression of bovine rotavirus vp8 and preparation of igy antibodies against recombinant vp8. | group a rotavirus is the leading cause of acute gastroenteritis in cattle and swine. although, vaccination against this virus is an effective strategy for prevention, additional strategy to control disease is necessary. egg yolk immunoglobulin (igy)-based passive immunization could be a better option in preventing this disease. bovine rotavirus (brv) is group a rotavirus and possesses a genome of 11 segments of double-stranded rna. the outer layer of capsid is composed of two proteins (vp7 and v ... | 2017 | 28523920 |
| epidemiology of rotavirus infection in children from a rural and urban area, in maputo, southern mozambique, before vaccine introduction. | this study aimed to describe the epidemiology of rotavirus infections in mozambique before vaccine introduction. between february 2012 and september 2013, stool specimens, demographic and clinical data were collected from 384 children <5 years old hospitalized with acute diarrhea in mavalane general hospital and manhiça district hospital, southern mozambique. the samples were tested for rotavirus a using enzyme-linked immunosorbent assay. the overall prevalence of rotavirus infection was 42.4% [ ... | 2017 | 28582541 |
| detection of uncommon g3p[3] rotavirus a (rva) strain in rat possessing a human rva-like vp6 and a novel nsp2 genotype. | rotavirus is one of the leading causes of acute gastroenteritis in infants and young children. rvas infect not only humans but also a wide range of mammals including rats, which represent a reservoir of several other zoonotic pathogens. due to the segmented nature of the rva genome, animal rva strains can easily adapt to the human host by reassortment with co-infecting human viruses. this study aims to detect and characterize rva in the intestinal content of italian sinantropic rats (rattus ratt ... | 2017 | 28603021 |
| phylogenetic analysis of g1p[8] and g12p[8] rotavirus a samples obtained in the pre- and post-vaccine periods, and molecular modeling of vp4 and vp7 proteins. | reduction in morbimortality rates for acute gastroenteritis (age) by rotavirus a (rva) has been observed after the introduction of vaccines, however the agent continues to circulate. the present study described the genomic characterization of the 11 dsrna segments of two rva samples g1p[8] obtained in the pre- and post-vaccination periods and one of g12p[8] sample (post-vaccine), compared to rotarix™ vaccine. analysis by molecular sequencing of the samples showed that the three samples belonged ... | 2017 | 28606817 |
| completely genomic and evolutionary characteristics of human-dominant g9p[8] group a rotavirus strains in yunnan, china. | g9p[8] rotavirus a (rva) has been identified as the predominant genotype circulating in yunnan, china. to elucidate the molecular characteristics of its genetic composition at the whole-genome level, the genomes of 12 strains isolated from paediatric patients with diarrhoea were fully sequenced and characterized. eleven of the 12 strains were genotyped as g9-p[8]-i1-r1-c1-m1-a1-n1-t1-e1-h1, which is closely related to the wa-like genotype 1 constellation. in contrast, one strain was genotyped as ... | 2017 | 28613141 |
| whole genome characterisation of a porcine-like human reassortant g26p[19] rotavirus a strain detected in a child hospitalised for diarrhoea in nepal, 2007. | a rare g26 rotavirus a strain rva/human-wt/npl/07n1760/2007/g26p[19] was detected in a child hospitalised for acute diarrhoea in kathmandu, nepal. the complete genome of 07n1760 was determined in order to explore its evolutionary history as well as examine its relationship to a vietnamese strain rva/human-wt/vnm/30378/2009/g26p[19], the only g26 strain whose complete genotype constellation is known. the genotype constellation of 07n1760 was g26-p[19]-i12-r1-c1-m1-a8-n1-t1-e1-h1, a unique constel ... | 2017 | 28673546 |
| reassortant ds-1-like g1p[4] rotavirus a strains generated from co-circulating strains in vietnam, 2012/2013. | one major mechanism by which rotavirus a (rva) evolves is genetic reassortment between strains with different genotype constellations. however, the parental strains of the reassortants generated have seldom been identified. here, the whole genome of two suspected reassortants, rva/human-wt/vnm/sp127/2013/g1p[4] and rva/human-wt/vnm/sp193/2013/g1p[4], with short rna electropherotypes were examined by illumina miseq sequencing and their ancestral phylogenies reconstructed. their genotype constella ... | 2017 | 28696017 |
| complex reassortment events of unusual g9p[4] rotavirus strains in india between 2011 and 2013. | rotavirus a (rva) is the predominant etiological agent of acute gastroenteritis in young children worldwide. recently, unusual g9p[4] rotavirus strains emerged with high prevalence in many countries. such intergenogroup reassortant strains highlight the ongoing spread of unusual rotavirus strains throughout asia. this study was undertaken to determine the whole genome of eleven unusual g9p[4] strains detected in india during 2011-2013, and to compare them with other human and animal global rvas ... | 2017 | 28750901 |
| group a rotavirus associated with encephalitis in red fox. | in 2011, a group a rotavirus was isolated from the brain of a fox with encephalitis and neurologic signs, detected by rabies surveillance in italy. intracerebral inoculation of fox brain homogenates into mice was fatal. genome sequencing revealed a heterologous rotavirus of avian origin, which could provide a model for investigating rotavirus neurovirulence. | 2017 | 28820385 |
| development of a multiplex real-time pcr assay for detection of human enteric viruses other than norovirus using samples collected from gastroenteritis patients in fukui prefecture, japan. | there are many varieties of gastroenteritis viruses, of which norovirus (nov) accounts for over 90% of the viral food poisoning incidents in japan. however, protocols for rapidly identifying other gastroenteritis viruses need to be established to investigate nov-negative cases intensively. in this study, a multiplex real-time pcr assay targeting rotavirus a, rotavirus c, sapovirus, astrovirus, adenovirus, and enterovirus was developed using stool samples collected from gastroenteritis patients b ... | 2017 | 28845896 |
| phylogenetic analysis of vp1 and rdrp genes of brazilian aichivirus b strains involved in a diarrhea outbreak in dairy calves. | aichivirus b has been reported worldwide in calves and adult cattle with and without diarrhea. the aim of this study was to describe the molecular characteristics of the rdrp and vp1 genes of aichivirus b strains identified as the most frequent etiologic agent in a neonatal diarrhea outbreak in a high-production brazilian dairy cattle herd. preliminary laboratory analysis ruled out important enteropathogens (cryptosporidium spp; eimeria spp., e. coli f5, and bovine coronavirus). fecal samples fr ... | 2017 | 28849283 |
| identification of two novel rotavirus a genotypes, g35 and p[50], from peruvian alpaca faeces. | rotavirus a (rva) alp11b was detected from a neonatal peruvian alpaca presenting with diarrhea, and the alp11b vp7, vp4, vp6, nsp4, and nsp5 genes were sequenced. the partial genotype constellation of this strain, rva/alpaca-wt/per/alp11b/2010, was determined to be g35-p[50]-i13-e16-h6. | 2017 | 28866138 |
| prevalence of lapine rotavirus, astrovirus, and hepatitis e virus in canadian domestic rabbit populations. | lapine rotavirus and astrovirus have been associated with disease in rabbits, and there is strong evidence of zoonotic transmission of lapine hepatitis e virus (hev). outbreaks of enteritis are common on commercial meat farms, resulting in poor welfare, high rabbit mortality, and significant financial losses for rabbit producers. currently, none of these viruses are routinely tested by diagnostic laboratories. in this study, we assessed the prevalence of rotavirus, astrovirus, and hev rna in 205 ... | 2017 | 28888629 |
| viral metagenomics of six bat species in close contact with humans in southern china. | accumulating studies have shown that bats could harbor various important pathogenic viruses that could be transmitted to humans and other animals. extensive metagenomic studies of different organs/tissues from bats have revealed a large number of novel or divergent viruses. to elucidate viral diversity and epidemiological and phylogenetic characteristics, six pooled fecal samples from bats were generated (based on bat species and geographic regions characteristic for virome analysis). these cont ... | 2018 | 28983731 |
| identification of group a rotaviruses from zambian fruit bats provides evidence for long-distance dispersal events in africa. | group a rotavirus (rva) is a major cause of diarrhea in children worldwide. although rva infects many animals, little is known about rva in bats. the present study investigated the genetic diversity of rva in zambian bats. we identified rva from two straw-colored fruit bats (eidolon helvum) and an egyptian fruit bat (rousettus aegyptiacus), and analyzed the genome sequences of these strains. genome segments of the rva strains from zambian e. helvum showed 97%-99% nucleotide sequence identity wit ... | 2018 | 29792990 |
| cynomolgus monkeys (macaca fascicularis) as an experimental infection model for human group a rotavirus. | group a rotaviruses (rva) are one of the most common causes of severe acute gastroenteritis in infants worldwide. rotaviruses spread from person to person, mainly by faecal⁻oral transmission. almost all unvaccinated children may become infected with rva in the first two years of life. the establishment of an experimental monkey model with rva is important to evaluate new therapeutic approaches. in this study, we demonstrated viral shedding and viraemia in juvenile⁻adult macaca fascicularis orall ... | 2018 | 29973483 |
| a novel group a rotavirus associated with acute illness and hepatic necrosis in pigeons (columba livia), in australia. | cases of vomiting and diarrhoea were reported in racing pigeons in western australia in may, 2016. morbidity and mortality rates were high. similar clinical disease was seen in victoria in december and by early 2017 had been reported in all states except the northern territory, in different classes of domestic pigeon-racing, fancy and meat bird-and in a flock of feral pigeons. autopsy findings were frequently unremarkable; histological examination demonstrated significant hepatic necrosis as the ... | 2018 | 30204797 |
| identification of a novel clade of group a rotaviruses in fatally diseased domestic pigeons in europe. | rotaviruses are well-known causative agents of enteric disorders in humans and other mammals, but little is known about their virulence and pathogenic role in pigeons and other birds. starting in summer 2017, a series of outbreaks of an acute disease with high mortalities was reported in domestic pigeons in germany, belgium and denmark. the clinical picture was characterized by diarrhoea, vomiting, hepatic necrosis and sudden fatalities. from these severe outbreaks, we discovered several previou ... | 2019 | 30407742 |
| cross-sectional study of the g and p genotypes of rotavirus a field strains circulating in regularly vaccinated dairy cattle herds. | neonatal diarrhea is the main cause of morbidity and mortality in calves up to 30 days old, and rotavirus a (rva) is the main viral etiology. rva vaccines are one of the main tools for diarrhea control in neonates. the aim of this cross-sectional study was to monitor by rt-pcr the g and p genotypes of rva strains identified in dairy cattle herds regularly vaccinated with the rva uk strain (g6p[5]). of the 14 randomly selected herds, two were excluded because no calf was diagnosed with diarrhea o ... | 2019 | 30535644 |
| sensitive sybr green-real time pcr for the detection and quantitation of avian rotavirus a. | avian rotavirus a (artv-a) is a virus that affects young birds, causing acute diarrhea and economic losses in the poultry industry worldwide. the techniques used for the diagnosis of artv-a include electron microscopy, isolation in cell culture, and serology, as well as molecular techniques, such as the reverse transcription-polymerase chain reaction (rt-pcr). the objective of this work was to standardize a real-time rt-polymerase chain reaction (rt-qpcr) using sybr green chemistry for the rapid ... | 2018 | 30597964 |
| longitudinal surveillance of rotavirus a genotypes circulating in a high milk yield dairy cattle herd after the introduction of a rotavirus vaccine. | worldwide, neonatal diarrhea is one of the most important health issues affecting dairy calves, and rotavirus a (rva) is one of its primary causes. among the measures to mitigate the risk of diarrhea outbreaks, cow vaccination stands out as one of the most important. however, the immune pressure resulting from routine vaccination may be able to select specific g and p genotypes in rva field strains. this study aimed to determine the frequency and intensity of neonatal diarrhea and the incidence ... | 2019 | 30827398 |
| leptospira spp., rotavirus, norovirus, and hepatitis e virus surveillance in a wild invasive golden-headed lion tamarin (leontopithecus chrysomelas; kuhl, 1820) population from an urban park in niterói, rio de janeiro, brazil. | the world currently faces severe biodiversity losses caused by anthropogenic activities such as deforestation, pollution, the introduction of exotic species, habitat fragmentation, and climate changes. disease ecology in altered environments is still poorly understood. the golden-headed lion tamarin (ghlt, leontopithecus chrysomelas) is an endangered species that became invasive in an urban park in niterói, rio de janeiro, brazil. the initially few invasive ghlt individuals became hundreds, adap ... | 2019 | 30828830 |
| genetic diversity and zoonotic potential of rotavirus a strains in the southern andean highlands, peru. | interspecies transmission is an important mechanism of evolution and contributes to rotavirus a (rva) diversity. in order to evaluate the detection frequency, genetic diversity, epidemiological characteristics and zoonotic potential of rva strains in faecal specimens from humans and animals cohabiting in the same environment in the department of cusco, peru, by molecular analysis, 265 faecal specimens were obtained from alpacas, llamas, sheep and shepherd children, and tested for rva by rt-pcr. ... | 2019 | 31002476 |
| the racing pigeon (columba livia domestica) industry in victoria, australia, and epidemiology of a novel group a rotavirus outbreak. | a novel group a rotavirus, first identified clinically in racing, feral and fancy pigeons in western australia, had spread throughout australia by march 2017. in december 2016, the putative index case of rotavirus in racing pigeons in the state of victoria was confirmed at a regional bird sale, with rapid spread to peri-urban melbourne, the capital city. a survey sent to approximately 400 victorian pigeon fanciers identified eight (of 85 respondents) with a confirmed rotavirus infection in their ... | 2019 | 31140207 |
| g and p genotype profiles of rotavirus a field strains circulating in beef and dairy cattle herds in brazil, 2006-2015. | the aim of this retrospective study was to use rt-pcr and nucleotide sequencing analysis to determine the g (vp7 gene) and p (vp4 gene) genotypes of 155 brazilian bovine rotavirus a (rva) wild-type strains detected in diarrheic calves from all brazilian geographical regions from 2006 to 2015. the rva strains evaluated belonged to the g6, g10, p[5], and p[11] genotypes. the g6p[5] genotype was prevalent (65.5%; p < 0.05) in beef, and the g10p[11] (38.4%) and g6p[11] (30.8%) genotypes were more pr ... | 2019 | 31174706 |