Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| a candidate packaging signal of human rotavirus differentiating wa-like and ds-1-like genomic constellations. | rotavirus a (rva) possesses a genome of 11 segmented rnas. in human rva, two major genomic constellations are represented by prototype strains wa and ds-1. here packaging signals differentiating wa-like and ds-1-like genomic constellations were searched for by analyzing genomic sequences of wa-like and ds-1-like strains. one pair of 11 nucleotide sites in the coding regions of viral structural protein (vp) 2 and vp6 was found to be complementary specifically among wa-like strains. these sites te ... | 2015 | 26224654 |
| detection and molecular characterization of caliciviruses (vesivirus and norovirus) in an outbreak of acute diarrhea in kittens from brazil. | feline caliciviruses (fcvs) have occasionally been described in cats in association with enteric disease, but an etiological role for these viruses in acute gastroenteritis is still unclear. in this study, molecular characterization of fcv and feline norovirus (fnov) was undertaken using real-time pcr (rt-pcr) and sequence analysis of the orf1 region in fecal specimens from 29 diarrheic cats. the specimens were also screened for parvovirus, coronavirus, astrovirus and group a rotavirus. a quanti ... | 2015 | 26189893 |
| full-genome sequence of the first g8p[14] rotavirus strain detected in the united states. | this is a report of the complete genomic sequence of a rare rotavirus group a g8-p[14]-i2-r3-c2-m2-a3-n2-t6-e2-h3 strain detected in a stool sample from a 57-year-old subject. | 2015 | 26089432 |
| etiological epidemiology of viral diarrhea on the basis of sentinel surveillance in children younger than 5 years in gansu, northwest china, 2009-2013. | to explore the etiological spectrum of diarrhea and its epidemiological characteristics in diarrhea symptoms surveillance cases younger than 5 years from 2009 to 2013 in gansu province, northwest china. systematic diarrhea symptoms surveillance were conducted in 27 sentinel sites in gansu province and outpatients with three or more loose, watery, or sticky pus stools per day were defined as surveillance cases. all stool specimens were tested for rotavirus, human calicivirus, adenovirus, and astr ... | 2015 | 26081875 |
| changes in the distribution of lineage constellations of g2p[4] rotavirus a strains detected in japan over 32 years (1980-2011). | rotavirus a (rva) is a leading cause of acute gastroenteritis in young children worldwide. most human rva strains are classified into three major genotype constellations: wa-like, ds-1-like and au-1-like. the evolution of g2p[4] strains possessing the ds-1-like genetic background was described in a few recent studies. however, the strains analyzed in these studies were almost exclusively the ones detected after 2000. in recognition of the scarcity of g2p[4] strains detected before 2000 for which ... | 2015 | 26026594 |
| rotavirus. | group a rotavirus (rva) is the major cause of acute gastroenteritis (age) in young children worldwide. introduction of two live, attenuated rotavirus vaccines, rotarix® and rotateq®, has dramatically reduced rva-associated age and mortality. high-throughput, sensitive and specific techniques are required to rapidly diagnose and characterize rotavirus strains in stool samples for proper patient treatment and to monitor circulating vaccine and wild-type rotavirus strains. new molecular assays are ... | 2015 | 26004648 |
| persistence of the rotavirus a genome in mesenteric lymph nodes of cattle raised on farms. | previous studies revealed that rotavirus a (rva) is present in not only the small intestine but also various organs. it was reported that rva persisted in mesenteric lymph nodes (mlns) in experimental models. however, there have been no reports focused on rva in mlns of animals under natural conditions. in this study, in order to investigate the persistence of the rva genome in mlns in cattle under natural conditions, reverse transcription-semi-nested pcr was carried out to detect rva genomes in ... | 2015 | 25991644 |
| group a rotavirus and norovirus genotypes circulating in the northeastern brazil in the post-monovalent vaccination era. | group a rotaviruses (rva) and noroviruses (nov) are the leading cause of acute gastroenteritis (age) worldwide. childhood diarrhea deaths and hospital admissions have declined since the introduction of the monovalent (g1p[8]) vaccine (rotarix(®) [rv1]) in the national immunization program in brazil in 2006. this study aims to investigate the epidemiological profile of nov and rva infections from children with age in the northeastern region of brazil in the post vaccine season. two-hundred fecal ... | 2015 | 25982627 |
| high prevalence and diversity of bovine astroviruses in the faeces of healthy and diarrhoeic calves in south west scotland. | astroviruses (astv) are single-stranded, positive-sense rna viruses and one of the major causes of infant diarrhoea worldwide. diarrhoea is a common and important cause of morbidity and mortality in calves; therefore, we investigated whether the presence of astv is associated with calf diarrhoea. we identified diverse astv lineages from faecal samples of both healthy and diarrhoeic calves and healthy adult cattle in south west scotland. astv was common in calves (present in 74% (85/115) of sampl ... | 2015 | 25979841 |
| rotavirus genotypes and the indigenous children of brazilian midwest in the vaccine era, 2008-2012: footprints of animal genome. | world group a rotavirus (rva) surveillance data provides useful estimates of the disease burden, however, indigenous population might require special consideration. the aim of this study was to describe the results of g- and p-types from brazilian native children ≤ 3 years. furthermore, selected strains have been analyzed for the vp7, vp6, vp4, and nsp4 encoding genes in order to gain insight into genetic variability of brazilian strains. a total of 149 samples, collected during 2008-2012, were ... | 2015 | 25963945 |
| rotavirus group a genotypes detected through diarrheal disease surveillance in haiti, 2012. | samples collected in 2012 through diarrheal disease surveillance in haiti were tested for rotavirus by enzyme immunoassay and real time rt-pcr and positive samples were genotyped. the predominant genotypes were g1p[8] (29% prevalence) and g9p[8] (21%). the observed genotype prevalence was similar to that reported previously for other caribbean countries. | 2015 | 25962775 |
| comparative characteristics of the vp7 and vp4 antigenic epitopes of the rotaviruses circulating in russia (nizhny novgorod) and the rotarix and rotateq vaccines. | two live, attenuated rotavirus a (rva) vaccines, rotarix and rotateq, have been successfully introduced into national immunization programs worldwide. the parent strains of both vaccines were obtained more than 30 years ago. nonetheless, only very limited data are available on the molecular similarity of the vaccine strains and their genetic relationships to the wild-type strains circulating within the territory of russian federation. in this study, we have determined the nucleotide sequences of ... | 2015 | 25944143 |
| evolution of a g6p[6] rotavirus strain isolated from a child with acute gastroenteritis in ghana, 2012. | unusual human g6p[6] rotavirus a (rva) strains have been reported sporadically in europe and africa, but how they evolved was not fully understood. the whole genome of a ghanaian g6p[6] strain designated pml1965 (2012) was analysed to understand how it evolved in africa and to learn how its g6 vp7 gene was related to that of rotaviruses of human and artiodactyl origin. the genotype constellation of rva/human-wt/gha/pml1965/2012/g6p[6] was g6-p-[6]-i2-r2-c2-m2-a2-n2-t2-e2-h2. it shared sublineage ... | 2015 | 25934790 |
| rotavirus vaccine effectiveness in latin american and caribbean countries: a systematic review and meta-analysis. | there are two group a rotavirus (rva) vaccines available worldwide since 2006: monovalent (rotarix(®), rv1) and pentavalent (rotateq(®), rv5). currently, 16 countries and 1 territory in latin america and the caribbean (lac) have introduced rva vaccines and since their introduction several impact and effectiveness studies have been conducted in different countries. the purpose of this study was to assess rva vaccine effectiveness in lac countries. | 2015 | 25919169 |
| genomic characterization of uncommon human g3p[6] rotavirus strains causing diarrhea in children in italy in 2009. | group a rotaviruses (rva) are the leading cause of acute gastroenteritis in young children, causing up to 450,000 deaths worldwide, mostly in developing countries. most of rva human infections in developed countries are related to five major g/p combinations: g1p[8], g2p[4], g3p[8], g4p[8] and g9p[8]. during the surveillance activity of rotanet-italy, three uncommon g3p[6] rva strains, designated as rva/human-wt/ita/na01/2009/g3p[6], rva/human-wt/ita/na06/2009/g3p[6], and rva/human-wt/ita/na19/2 ... | 2015 | 25913157 |
| detection and characterization of a human g9p[4] rotavirus strain in japan. | in a surveillance system in osaka city, japan, 48 sporadic rotavirus a (rva) infections were detected during 2008/2009-2011/2012 seasons. the g/p-genotypes of detected rvas were g1p[8], g2p[4], g3p[8], g9p[4], and g9p[8]. although g9p[4] is a rare genotype that had not been reported in japan, it was the second most prevalent genotype, following g1p[8], and accounted for 35.3% of rva cases in the 2011/2012 season. further genotyping revealed that the g9p[4] strain had genotype 2 internal protein ... | 2015 | 25907907 |
| genomic characterization of g3p[6], g4p[6] and g4p[8] human rotaviruses from wuhan, china: evidence for interspecies transmission and reassortment events. | we report here the whole genomic analyses of two g4p[6] (rva/human-wt/chn/e931/2008/g4p[6], rva/human-wt/chn/r1954/2013/g4p[6]), one g3p[6] (rva/human-wt/chn/r946/2006/g3p[6]) and one g4p[8] (rva/human-wt/chn/e2484/2011/g4p[8]) group a rotavirus (rva) strains detected in sporadic cases of diarrhea in humans in the city of wuhan, china. all the four strains displayed a wa-like genotype constellation. strains e931 and r1954 shared a g4-p[6]-i1-r1-c1-m1-a8-n1-t1-e1-h1 constellation, whilst the 11 g ... | 2015 | 25891280 |
| evolution of human g4p[8] group a rotavirus strains circulating in italy in 2013. | group a rotaviruses (rva) are the leading cause of acute gastroenteritis in young (<5 years of age) children, causing up to 450.000 deaths worldwide, mostly in developing countries. vp7 (g-type) and vp4 (p-type) genotypes are the basis for the binary rva classification. although at least 27 g-types and 37 p-types of rotavirus are presently known, most rva infections in humans worldwide are associated with the five major g/p combinations g1p[8], g2p[4], g3p[8], g4p[8] and g9p[8]. during rva gastr ... | 2015 | 25889745 |
| unusual rotavirus genotypes among children with acute diarrhea in saudi arabia. | human rotavirus a (human rv-a) is the most common cause of viral gastroenteritis in infants. the objective of the study was to characterize the g and p genotypes among clinical rotavirus isolates from children with acute diarrhea admitted to a tertiary care hospital in riyadh, saudi arabia. | 2015 | 25884670 |
| a prospective hospital-based surveillance to estimate rotavirus disease burden in bhutanese children under 5 years of age. | as part of efforts to develop an informed policy for rotavirus vaccination, this prospective study was conducted to estimate the burden of rotavirus diarrhea among children less than 5 years old attended to the department of pediatrics, jigme dorji wangchuk national referral hospital (jdwnrh), thimphu, bhutan. the duration of the study was three years, extending from february 2010 through december 2012. we estimated the frequency of hospitalization in the pediatric ward and dehydration treatment ... | 2015 | 25859154 |
| intrasegmental recombination does not contribute to the long-term evolution of group a rotavirus. | rotavirus is a genetically diverse pathogen with an eleven-segmented, double-stranded rna genome. intrasegmental recombination has been proposed as a potential mechanism to generate antigenic diversity and a possible route of escape from vaccine-imposed selective pressure. here intrasegmental recombination was studied by performing a genome-wide scan across the eleven genome segments of 797 publically available rotavirus strains. sixty-two sequences, or 0.7% of sequences analyzed, have evidence ... | 2015 | 25847696 |
| use of s1 nuclease in deep sequencing for detection of double-stranded rna viruses. | metagenomic approach using next-generation dna sequencing has facilitated the detection of many pathogenic viruses from fecal samples. however, in many cases, majority of the detected sequences originate from the host genome and bacterial flora in the gut. here, to improve efficiency of the detection of double-stranded (ds) rna viruses from samples, we evaluated the applicability of s1 nuclease on deep sequencing. treating total rna with s1 nuclease resulted in 1.5-28.4- and 10.1-208.9-fold incr ... | 2015 | 25843154 |
| large scale survey of enteric viruses in river and waste water underlines the health status of the local population. | although enteric viruses constitute a major cause of acute waterborne diseases worldwide, environmental data about occurrence and viral load of enteric viruses in water are not often available. in this study, enteric viruses (i.e., adenovirus, aichivirus, astrovirus, cosavirus, enterovirus, hepatitis a and e viruses, norovirus of genogroups i and ii, rotavirus a and salivirus) were monitored in the seine river and the origin of contamination was untangled. a total of 275 water samples were colle ... | 2015 | 25795193 |
| genomic characterization and molecular investigation of vp7 epitopes of uncommon g10p[8] group a rotavirus strains detected in italy in 2009. | rotavirus strains with the uncommon genotype g10 have been detected sporadically in cases of acute gastroenteritis in humans and are thought to be transmitted zoonotically. during 2009, 10 g10p[8] rotavirus strains were detected in the stools of children hospitalized with acute diarrhoea in several paediatric hospitals in italy. the phylogenetic analysis of the vp7 gene of the italian g10p[8] strains analysed revealed nucleotide identities ranging from 94 to 99 %. molecular characterization of t ... | 2015 | 25783473 |
| molecular characterization of group a rotavirus strains detected in children with diarrhea admitted to nigerian hospitals in 2013. | group a rotaviruses (rva) are the leading cause of acute gastroenteritis (age) in children worldwide and cause up to 455,000 deaths annually, mostly in developing countries. during 2013, 66 rvas from children with age admitted to four nigerian hospitals were investigated. the g3p[6], g1p[8] and g2p[4] genotypes predominated. the vp7 and/or vp4 genes of 18 g3p[6]/[8]/[4], six g2p[4], three g12p[8]/[4], and two g1p[8] rva strains were sequenced. the g3p[6] strains belonged to lineage g3-iii and we ... | 2015 | 25772575 |
| complete genomic sequence for an avian group g rotavirus from south africa. | we report the first complete sequence for an avian group g rotavirus (rvg) genome from africa, which is the third publically available rvg genome. these rvg genomes are highly diverse, especially in their vp4, vp7, nsp4, and nsp3 segments, indicating that rvg diversity is comparable to that of rotavirus a. | 2015 | 25767240 |
| unexpected spreading of g12p[8] rotavirus strains among young children in a small area of central italy. | rotavirus gastroenteritis is associated mainly with the five genotypes g1,3,4,9p[8] and g2p[4] that are common worldwide, but emerging strains including g6, g8, and g12 are also reported sporadically. g12p[8] rotavirus was observed unexpectedly to spread in a limited area of italy during the rotavirus surveillance season 2012-2013. all strains were genotyped for vp7 and vp4 and subjected to phylogenetic analysis. amino acid sequences of antigenic regions were compared with vaccine and field stra ... | 2015 | 25758365 |
| human rotavirus group a serotypes causing gastroenteritis in children less than 5 years and hiv-infected adults in viwandani slum, nairobi. | rotavirus remains a leading cause of acute gastroenteritis in children worldwide with an estimated 2000 deaths each day in developing countries. due to hiv/aids scourge in kenya, it is possible that rotavirus-related gastroenteritis has been aggravated in adults. the global alliance for immunizations has ranked rotavirus infection a priority for vaccine, and, to ensure its success, there is a need to document the local strain(s) circulating in different regions. | 2015 | 25733783 |
| h2 genotypes of g4p[6], g5p[7], and g9[23] porcine rotaviruses show super-short rna electropherotypes. | during group a rotavirus (rva) surveillance of pig farms in japan, we detected three rva strains (g4p[6], g5p[7], and g9p[23] genotypes), which showed super-short rna patterns by polyacrylamide gel electrophoresis, in samples from a healthy eight-day-old pig and two pigs of seven and eight days old with diarrhea from three farms. reverse transcription pcr and sequencing revealed that the full-length nsp5 gene of these strains contained 952 or 945 nucleotides, which is consistent with their super ... | 2015 | 25724331 |
| whole-genome sequence analysis of g3 and g14 equine group a rotaviruses isolated in the late 1990s and 2009-2010. | equine group a rotavirus (rva) g3p[12] and g14p[12] strains cause gastroenteritis in foals worldwide. both of these strains have been co-circulating in japan since g14p[12] strains emerged in the late 1990s. although it is important to comprehensively understand the evolution of rva strains, whole-genome sequence data on recent equine rva strains in japan are lacking. therefore, in this study, whole-genome analysis of 23 equine rva isolates from the late 1990s and 2009-2010 and the vaccine strai ... | 2015 | 25708838 |
| changing patterns of rotavirus strains circulating in ireland: re-emergence of g2p[4] and identification of novel genotypes in ireland. | worldwide, group a rotavirus (rva) is recognized as the most common aetiological agent of acute diarrheal disease in children. one hundred and ninety seven positive faecal samples were obtained from patients between 2006 and 2008. reverse transcriptase polymerase chain reaction (rt-pcr) was used to amplify the vp7 and vp4 gene segments of these samples, and g and p typing was carried out subsequently. the most common strain type was g1p[8], and the emergent global g9-type was identified in both ... | 2015 | 25704492 |
| detection of avian-like rotavirus a vp4 from a calf in japan. | a total of 568 normal feces from calves on a beef farm in fukui prefecture, japan, in 2011-2012 were examined by rt-semi-nested pcr for rotavirus a (rva) vp4 genes. through partial sequencing and blast analyses of 84 vp4-positive specimens, we identified an avian-like rva strain, n2342, which shares highest nucleotide identity (80.0%) with known avian-like bovine strain 993/83, in one specimen. phylogenetic analysis also revealed a close genetic relationship between n2342 and avian rvas, suggest ... | 2015 | 25311984 |
| a bovine g8p[1] group a rotavirus isolated from an asymptomatically infected dog. | group a rotaviruses (rvas) are enteric pathogens with well-documented zoonotic transmissions to humans. the segmented genome of the virus enables reassortment events which might alter host susceptibility and/or disease course. genetic analysis of rotavirus in dogs has so far only revealed rvas with the vp7 and vp4 genome constellation g3p[3]. rva g3p[3] have also been found in cats, humans, monkeys and bats. in the present study, we described an unusual rva of genotype g8p[1] which was isolated ... | 2015 | 25304653 |
| molecular epidemiology of rotavirus in cats in the united kingdom. | rotaviruses are leading causes of gastroenteritis in the young of many species. molecular epidemiological studies in children suggest that interspecies transmission contributes to rotavirus strain diversity in people. however, population-based studies of rotaviruses in animals are few. we investigated the prevalence, risk factors for infection, and genetic diversity of rotavirus a in a cross-sectional survey of cats housed within 25 rescue catteries across the united kingdom. morning litter tray ... | 2015 | 25411173 |
| an outbreak of acute gastroenteritis associated with group a rotavirus in long-term care facility in slovenia. | in april 2013, an outbreak of acute gastroenteritis was reported in a residential long-term care facility (ltcf) in ljubljana. we carried out an outbreak investigation to describe the outbreak, identify factors associated with contracting infection and to recommend control measures. | 2015 | 25447968 |
| isothermal cross-priming amplification implementation study. | cross-priming amplification (cpa) was evaluated for the early detection of norovirus (nv), rotavirus a (rv-a), enteric adenovirus (eadv) and astrovirus (astv). the analytical sensitivity of the cpa assay was 10(3) copies ml(-1) for nv, rv-a and astv detection and 10(4) copies ml(-1) for eadv detection. for each of the four pathogens, the positive detection rate by cpa was similar to real-time pcr methods and higher than the rate observed in an elisa. the detection coincidence rates of cpa and rt ... | 2015 | 25346035 |
| rapid and simultaneous detection of three major diarrhea-causing viruses by multiplex real-time nucleic acid sequence-based amplification. | rotaviruses, noroviruses and astroviruses are the major viral pathogens leading to diarrhea worldwide. epidemiological investigations of outbreaks associated with these viruses have been impeded by the lack of methods for quick diagnosis and detection. in the current study, a multiplex real-time nucleic acid sequence-based amplification (rt-nasba) system was developed for the simultaneous detection of rotavirus a/norovirus genogroup ii/astrovirus. the specificity and sensitivity of the assay wer ... | 2015 | 25559674 |
| molecular epidemiology of enteric viruses and genotyping of rotavirus a, adenovirus and astrovirus among children under 5 years old in gabon. | this study aimed to determine the prevalence of enteric viruses causing gastroenteritis, and the circulating stains, in gabonese children under five years old who visited health centers between march 2010 and june 2011. | 2015 | 25796432 |
| evaluation of molecular- and culture-dependent mst markers to detect fecal contamination and indicate viral presence in good quality groundwater. | microbial contamination of groundwater represents a significant health risk to resource users. culture-dependent bacteroides phage and molecular-dependent bacteroidales 16s rrna assays are employed in microbial source tracking (mst) studies globally, however little is known regarding how these important groups relate to each other in the environment and which is more suitable to indicate the presence of waterborne fecal pollution and human enteric viruses. this study addresses this knowledge gap ... | 2015 | 25871525 |
| human and animal enteric virus in groundwater from deep wells, and recreational and network water. | this study was designed to assess the presence of human adenovirus (hadv), rotavirus-a (rva), hepatitis a virus (hav), and porcine circovirus-2 (pcv2) in groundwater from deep wells, and recreational and network waters. the water samples were collected and concentrated and the virus genomes were assessed and quantified by quantitative pcr (qpcr). infectious hadv was evaluated in groundwater and network water samples by integrated cell culture using transcribed messenger rna (mrna) (icc-rt-qpcr). ... | 2015 | 26300358 |
| detection and molecular characterization of zoonotic viruses in swine fecal samples in italian pig herds. | gastrointestinal disease is frequent in pigs, and among the different etiological agents involved, viruses are considered the leading cause of infection in this animal species. furthermore, about half of the newly identified swine pathogens are viruses, many of which may be transmitted to humans by direct contact or by indirect transmission pathways. in this study, the prevalence of astrovirus (astv), group a rotavirus (rva), norovirus (nov) and hepatitis e virus (hev) infections in pigs was inv ... | 2015 | 26215443 |
| detection and characterisation of novel bocavirus (genus bocaparvovirus) and gastroenteritis viruses from asymptomatic pigs in ireland. | livestock animals have been the assumed source of several human epidemics in recent years, for example, influenza h1n1, rotavirus g8/g9, and mers-cov. surveillance of novel viruses in animals is essential to evaluate the risk to human and animal health and to determine any economic impact, for example, failure to thrive. there is a paucity of data regarding detection and characterisation of gastroenteritis viruses, particularly novel viruses, in porcines in ireland. recently, a number of small n ... | 2015 | 26065833 |
| two-year monitoring of water samples from dam of iskar and the black sea, bulgaria, by molecular analysis: focus on mycobacterium spp. | the coast of the bulgarian black sea is a popular summer holiday destination. the dam of iskar is the largest artificial dam in bulgaria, with a capacity of 675 million m3. it is the main source of tap water for the capital sofia and for irrigating the surrounding valley. there is a close relationship between the quality of aquatic ecosystems and human health as many infections are waterborne. rapid molecular methods for the analysis of highly pathogenic bacteria have been developed for monitori ... | 2015 | 26133133 |
| aetiology of acute paediatric gastroenteritis in bulgaria during summer months: prevalence of viral infections. | paediatric acute gastroenteritis is a global public health problem. comprehensive laboratory investigation for viral, bacterial and parasitic agents is helpful for improving management of acute gastroenteritis in health care settings and for monitoring and controlling the spread of these infections. our study aimed to investigate the role of various pathogens in infantile diarrhoea in bulgaria outside the classical winter epidemics of rotavirus and norovirus. stool samples from 115 hospitalized ... | 2015 | 25596126 |
| generation of an avian-mammalian rotavirus reassortant by using a helper virus-dependent reverse genetics system. | the genetic diversity of rotavirus a (rva) strains is facilitated in part by genetic reassortment. although this process of genome segment exchange has been reported frequently among mammalian rvas, it remained unknown if mammalian rvas also could package genome segments from avian rva strains. we generated a simian rva strain sa11 reassortant containing the vp4 gene of chicken rva strain 02v0002g3. to achieve this, we transfected bsr5/t7 cells with a t7 polymerase-driven vp4-encoding plasmid, i ... | 2015 | 26581988 |
| [the application and epidemiological research of xtag gpp multiplex pcr in the diagnosis of infectious diarrhea]. | to investigate the application value of xtag (®) gastrointestinal pathogen panel (xtag9(®) gpp) multiplex pcr in the early diagnosis of infectious diarrhea, and understand the epidemiology of intestinal diarrhea pathogens. | 2015 | 25916780 |
| prevalence of endemic enteropathogens of calves in new zealand dairy farms. | to conduct a country-wide prevalence study of bovine group a rotavirus, coronavirus, cryptosporidium parvum, salmonella spp. and enterotoxigenic k99(+) escherichia coli (k99) in calves on new zealand dairy farms. | 2015 | 25237728 |
| genotyping and determining the distribution of prevalent g and p types of group a bovine rotaviruses between 2010 and 2012 in iran. | determination and distribution of the g and p genotypes of group a bovine rotavirus was investigated on 386 fecal samples collected from calves with diarrhea using a semi-nested rt-pcr typing assay. samples were collected from 11 provinces of iran during 2010-2012. the provinces divided into 5 different groups based on geographical distance and climates. one hundred and nine samples (28.2%) were confirmed positive for rotavirus group a using elisa. 75 positive samples were selected randomly and ... | 2015 | 26072368 |
| norovirus and rotavirus survival in urine collected from a public ecological sanitation system in ouagadougou, burkina faso. | urine from urine-diversion toilets (udts) is routinely used as fertilizer for urban agriculture in ouagadougou, burkina faso. because urine from udts can be accidentally spoiled by feces, we determined whether virulent enteric viruses could persist in urine that is used for agricultural purposes and pose a threat to human health. urine samples (n = 60) were first collected from 42 udts during the months of january and february 2012 in ouagadougou and screened negative for the presence of norovir ... | 2015 | 25408534 |
| phylogenetic analysis of a g6p[5] bovine rotavirus strain isolated in a neonatal diarrhea outbreak in a beef cattle herd vaccinated with g6p[1] and g10p[11] genotypes. | the aim of this study was to perform the molecular characterization of the eleven genes of a g6p[5] bovine group a rotavirus (rva) strain detected in a diarrhea outbreak from a vaccinated beef cattle herd. the outbreak affected 80 % of calves between 15-30 days old. rva was identified by rt-pcr in 12 (70.6 %) out of 17 diarrheic fecal samples evaluated. the rotavirus wild-type strain had the genotype constellation g6(iv)-p[5](ix)-i2c-r2-c2-m2-a3-n2-t6-e2e-h3a. this study confirms the importance ... | 2015 | 25377636 |
| full-genome sequencing of a hungarian canine g3p[3] rotavirus a strain reveals high genetic relatedness with a historic italian human strain. | a canine rotavirus a strain was identified in the fecal specimen of a young dog during 2012 in hungary. the strain rva/dog-wt/hun/135/2012/g3p[3] shared complete genotype constellation (g3-p[3]-i3-r3-c3-m3-a15-n2-t3-e3-h6) and high genome sequence similarity (nt, 98.8 %) with a historic human strain, rva/human-tc/ita/pa260-97/1997/g3p[3]. this study provides evidence for the canine origin of the unusual nsp1 genotype, a15, and reinforces the hypothesis of direct interspecies transmission of cani ... | 2015 | 25634124 |
| whole genotype constellation of prototype feline rotavirus strains frv-1 and frv64 and their phylogenetic relationships with feline-like human rotavirus strains. | feline rotaviruses, members of the species rotavirus a, are an infrequent source of zoonotic infections, and were previously shown by rna-rna hybridization assays to possess two distinct genomic rna constellations, represented by strains frv-1 and frv64. due to the lack of whole genome sequence information for frv-1, human rotavirus strain au-1 has been used as a surrogate for the genotype constellation of feline rotaviruses. the aim of this study was to determine the whole genome sequence of fr ... | 2015 | 25351516 |
| microbial aetiology of acute diarrhoea in children under five years of age in khartoum, sudan. | diarrhoea is one of leading causes of morbidity and mortality worldwide. recent estimations suggested the number of deaths is close to 2.5 million. this study examined the causative agents of diarrhoea in children under 5 years of age in suburban areas of khartoum, sudan. a total of 437 stool samples obtained from children with diarrhoea were examined by culture and pcr for bacteria, by microscopy and pcr for parasites and by immunoassay for detection of rotavirus a. of the 437 samples analysed, ... | 2015 | 25713206 |
| structural basis for 2'-5'-oligoadenylate binding and enzyme activity of a viral rnase l antagonist. | synthesis of 2'-5'-oligoadenylates (2-5a) by oligoadenylate synthetase (oas) is an important innate cellular response that limits viral replication by activating the latent cellular rnase, rnase l, to degrade single-stranded rna. some rotaviruses and coronaviruses antagonize the oas/rnase l pathway through the activity of an encoded 2h phosphoesterase domain that cleaves 2-5a. these viral 2h phosphoesterases are phylogenetically related to the cellular a kinase anchoring protein 7 (akap7) and sh ... | 2015 | 25878106 |
| crystal structure of the c-terminal 2',5'-phosphodiesterase domain of group a rotavirus protein vp3. | in response to viral infections, the mammalian innate immune system induces the production of the second messenger 2'-5' oligoadenylate (2-5a) to activate latent ribonuclease l (rnase l) that restricts viral replication and promotes apoptosis. a subset of rotaviruses and coronaviruses encode 2',5'-phosphodiesterase enzymes that hydrolyze 2-5a, thereby inhibiting rnase l activation. we report the crystal structure of the 2',5'-phosphodiesterase domain of group a rotavirus protein vp3 at 1.39 å re ... | 2015 | 25758703 |
| multicenter evaluation of the biofire filmarray gastrointestinal panel for etiologic diagnosis of infectious gastroenteritis. | the appropriate treatment and control of infectious gastroenteritis depend on the ability to rapidly detect the wide range of etiologic agents associated with the disease. clinical laboratories currently utilize an array of different methodologies to test for bacterial, parasitic, and viral causes of gastroenteritis, a strategy that suffers from poor sensitivity, potentially long turnaround times, and complicated ordering practices and workflows. additionally, there are limited or no testing met ... | 2015 | 25588652 |
| virus variation resource - improved response to emergent viral outbreaks. | the virus variation resource is a value-added viral sequence data resource hosted by the national center for biotechnology information. the resource is located at http://www.ncbi.nlm.nih.gov/genome/viruses/variation/ and includes modules for seven viral groups: influenza virus, dengue virus, west nile virus, ebolavirus, mers coronavirus, rotavirus a and zika virus each module is supported by pipelines that scan newly released genbank records, annotate genes and proteins and parse sample descript ... | 2016 | 27899678 |
| detection of a novel circovirus pcv3 in pigs with cardiac and multi-systemic inflammation. | porcine circovirus 2 causes different clinical syndromes resulting in a significant economic loss in the pork industry. three pigs with unexplained cardiac and multi-organ inflammation that tested negative for pcv2 and other known porcine pathogens were further analyzed. | 2016 | 27835942 |
| case-control study of pathogens involved in piglet diarrhea. | diarrhea in piglets directly affects commercial swine production. the disease results from the interaction of pathogens with the host immune system and is also affected by management procedures. several pathogenic agents such as campylobacter spp., clostridium perfringens, escherichia coli, salmonella spp., group a rotavirus (rv-a), coronaviruses (transmissible gastroenteritis virus; porcine epidemic diarrhea virus), as well as nematode and protozoan parasites, can be associated with disease cas ... | 2016 | 26754836 |
| presence and characterization of pig group a and c rotaviruses in feces of belgian diarrheic suckling piglets. | the importance of group a and c rotaviruses (rva and rvc) in the pathogenesis of diarrhea in belgian suckling pigs is poorly investigated, and it is not known which strains are circulating in the belgian suckling pig population. obtaining better insights in the occurrence of both viral species in the swine population is essential in order to develop accurate diagnostic, therapeutic and prophylactic strategies to protect suckling pigs against diarrhea in a durable manner. in the present study, vi ... | 2016 | 26677793 |
| whole genome characterization of a g6p[5] rotavirus a strain isolated from a stray cat in japan. | the whole genome of an unusual g6p[5] rotavirus a strain named frv537, which was isolated from a stray cat in japan, was characterized to determine its species of origin. the genotype constellation of frv537 was g6-p[5]-i2-r2-c2-m2-a13-n2- t6-e2-h3. no known feline rotavirus has this genotype constellation; the japanese equine strain oh-4 is the only known strain that does. while frv537 shares the same genotype with some feline rotaviruses in all genes except those encoding vp4 and nsp1, none of ... | 2016 | 27139026 |
| multispecies reassortant bovine rotavirus strain carries a novel simian g3-like vp7 genotype. | rotavirus-a (rvas), are the major cause of severe gastroenteritis in the young of mammals and birds. rva strains possessing g6, g8, and g10 genotypes in combination with p[1] or p[11] have been commonly detected in cattle. during a routine surveillance for enteric viruses in a bovine population on north-western temperate himalayan region of india, an uncommon bovine rva strain, designated as rva/cow-wt/ind/m1/09/2009 was detected in a diarrhoeic crossbred calf. the examination of nearly complete ... | 2016 | 27033751 |
| human and bovine viruses and bacteria at three great lakes beaches: environmental variable associations and health risk. | waterborne pathogens were measured at three beaches in lake michigan, environmental factors for predicting pathogen concentrations were identified, and the risk of swimmer infection and illness was estimated. waterborne pathogens were detected in 96% of samples collected at three lake michigan beaches in summer, 2010. samples were quantified for 22 pathogens in four microbial categories (human viruses, bovine viruses, protozoa, and pathogenic bacteria). all beaches had detections of human and bo ... | 2016 | 26720156 |
| cosavirus, salivirus and bufavirus in diarrheal tunisian infants. | three newly discovered viruses have been recently described in diarrheal patients: cosavirus (cosv) and salivirus (salv), two picornaviruses, and bufavirus (buv), a parvovirus. the detection rate and the role of these viruses remain to be established in acute gastroenteritis (age) in diarrheal tunisian infants. from october 2010 through march 2012, stool samples were collected from 203 children <5 years-old suffering from age and attending the children's hospital in monastir, tunisia. all sample ... | 2016 | 27631733 |
| virus reduction during advanced bardenpho and conventional wastewater treatment processes. | the present study investigated wastewater treatment for the removal of 11 different virus types (pepper mild mottle virus; aichi virus; genogroup i, ii, and iv noroviruses; enterovirus; sapovirus; group-a rotavirus; adenovirus; and jc and bk polyomaviruses) by two wastewater treatment facilities utilizing advanced bardenpho technology and compared the results with conventional treatment processes. to our knowledge, this is the first study comparing full-scale treatment processes that all receive ... | 2016 | 27447291 |
| case-control study of diarrheal disease etiology in individuals over 5 years in southwest china. | acute diarrhea is one of the major public health problems worldwide. most of studies on acute diarrhea have been made on infants aged below 5 years and few efforts have been made to identify the etiological agents of acute diarrhea in people over five, especially in china. | 2016 | 27891182 |
| impact of co-infections with enteric pathogens on children suffering from acute diarrhea in southwest china. | acute diarrhea is a global health problem, resulting in high morbidity and mortality in children. it has been suggested that enteric pathogen co-infections play an important role in gastroenteritis, but most research efforts have only focused on a small range of species belonging to a few pathogen groups. this study aimed to assess the impact of co-infections with a broad range of enteric pathogens on children aged below five years who suffer from acute diarrhea in southwest china. | 2016 | 27349521 |
| a recombinant nucleocapsid protein-based indirect enzyme-linked immunosorbent assay to detect antibodies against porcine deltacoronavirus. | recently, porcine deltacoronavirus (pdcov) has been proven to be associated with enteric disease in piglets. diagnostic tools for serological surveys of pdcov remain in the developmental stage when compared with those for other porcine coronaviruses. in our study, an indirect enzyme-linked immunosorbent assay (elisa) (rpdcov-n-elisa) was developed to detect antibodies against pdcov using a histidine-tagged recombinant nucleocapsid (n) protein as an antigen. the rpdcov-n-elisa did not cross-react ... | 2016 | 26668175 |
| diarrheagenic pathogens in adults attending a hospital in singapore. | singapore's diarrhoeal notification system is based on specific pathogens. official data may thus be skewed towards notifiable diseases. limited information is available on the profiles of aetiological agents responsible for acute gastroenteritis (age) cases, especially among the adult population. to understand the frequency and distribution of potential causative agents of diarrheal disease in singapore, we screened adults' stool samples collected from a large public hospital. | 2016 | 26822615 |
| epidemiological survey of enteric viruses in wild boars in the czech republic: first evidence of close relationship between wild boar and human rotavirus a strains. | population of wild boar is increasing in the whole europe, the animals migrate close to human habitats which greatly increases the possibility of natural transmission between domestic animals or humans and wild boars. the aim of the study was to estimate in population of free-living wild boar in the czech republic the prevalence of enteric viral pathogens, namely rotavirus groups a and c (rva and rvc), porcine reproductive and respiratory syndrome virus (prrsv), and members of family coronavirid ... | 2016 | 27599927 |
| propidium monoazide coupled with pcr predicts infectivity of enteric viruses in swine manure and biofertilized soil. | the use of propidium monoazide (pma) coupled with real-time pcr (rt-qpcr or qpcr for rna or dna viruses, respectively) was assessed to discriminate infectious enteric viruses in swine raw manure, swine effluent from anaerobic biodigester (ab) and biofertilized soils. those samples were spiked either with infectious and heat-inactivated human adenovirus-2 (hadv-2) or mengovirus (vmc0), and pma-qpcr/rt-qpcr allowed discriminating inactivated viruses from the infective particles, with significant r ... | 2016 | 26742766 |
| molecular epidemiology of enteric viruses in patients with acute gastroenteritis in aichi prefecture, japan, 2008/09-2013/14. | acute gastroenteritis is a critical infectious disease that affects infants and young children throughout the world, including japan. this retrospective study was conducted from september 2008 to august 2014 (six seasons: 2008/09-2013/14) to investigate the incidence of enteric viruses responsible for 1,871 cases of acute gastroenteritis in aichi prefecture, japan. of the 1,871 cases, 1,100 enteric viruses were detected in 978 samples, of which strains from norovirus (nov) genogroup ii (60.9%) w ... | 2016 | 26647761 |
| viruses surveillance under different season scenarios of the negro river basin, amazonia, brazil. | the negro river is located in the amazon basin, the largest hydrological catchment in the world. its water is used for drinking, domestic activities, recreation and transportation and water quality is significantly affected by anthropogenic impacts. the goals of this study were to determine the presence and concentrations of the main viral etiological agents of acute gastroenteritis, such as group a rotavirus (rva) and genogroup ii norovirus (nov gii), and to assess the use of human adenovirus ( ... | 2016 | 26783031 |
| development of one-step real-time reverse transcriptase-pcr-based assays for the rapid and simultaneous detection of four viruses causing porcine diarrhea. | porcine diarrhea caused by viruses is a major problem of the pig farming industry and can result in substantial losses of revenue. thus, diagnosing the infectious agents is important to prevent and control diseases in pigs. we developed novel one-step real-time quantitative rt-pcr (qpcr) assays that can detect four porcine diarrheal viruses simultaneously: porcine epidemic diarrhea virus (pedv), transmissible gastroenteritis virus (tgev), porcine deltacoronavirus (pdcov), and porcine group a rot ... | 2016 | 27348884 |
| the interaction of rotavirus a pig/china/nmtl/2008/g9p[23] vp6 with cellular beta-actin is required for optimal rv replication and infectivity. | vp6 forms the intermediate layer of the rotavirus (rv) capsid, and it plays important roles after rv penetration and uncoating. these functions rely on its ability to interact with host cell proteins. to gain further insights into the role of vp6 in porcine rv (porv) infection, a glutathione s-transferase pull-down assay was utilized to find unknown cellular factors that interact with vp6. in this study, beta-actin, tropomyosin 1, and 40s ribosomal protein s16 were identified as interaction part ... | 2016 | 27938672 |
| characterization of a genetically heterogeneous porcine rotavirus c, and other viruses present in the fecal virome of a non-diarrheic belgian piglet. | next-generation sequencing (ngs) technologies are becoming increasingly accessible, leading to an expanded interest in the composition of the porcine enteric virome. in the present study, the fecal virome of a non-diarrheic belgian piglet was determined. although the virome of only a single piglet was analyzed, some interesting data were obtained, including the second complete genome of a pig group c rotavirus (rvc). this belgian strain was only distantly related to the only other completely cha ... | 2016 | 27184192 |
| phylogenetic inference of the porcine rotavirus a origin of the human g1 vp7 gene. | rotavirus a (rva) is an important cause of acute gastroenteritis in children worldwide. the most common vp7 genotype of human rva is g1, but g1 is rarely detected in porcine strains. to understand the evolutionary relationships between human and porcine g1 vp7 genes, we sequenced the vp7 genes of three japanese g1 porcine strains; the first two (prv2, s80b) were isolated in 1980 and the third (kyusyu-14) was isolated in 2001. then, we performed phylogenetic and in-silico structural analyses. all ... | 2016 | 26961591 |
| shedding of porcine circovirus type 1 dna and rotavirus rna by infants vaccinated with rotarix®. | thirty-three infants aged ∼2 months had serial stool samples collected after receipt of rotarix® vaccine dose 1, and were assessed for shedding of porcine circovirus type 1 dna and rotavirus group a rna by molecular methods. we did not find strong evidence that porcine circovirus type 1 replication occurred. porcine circovirus type 1 genome with the same sequence as that in rotarix® was detected in a few infants as late as day ≥ 13; while this timing could suggest there may have been replication ... | 2016 | 27936349 |
| molecular detection of porcine astrovirus in sichuan province, china. | porcine astrovirus (poastv) is widely distributed worldwide, and is highly prevalent among piglets with or without diarrhea, existing as at least five distinct lineages (poastv1-poastv5) within the genus mamastrovirus. however, our knowledge of the diversity and epidemiology of poastv in china is limited. | 2016 | 26739067 |
| molecular investigations on the prevalence and viral load of enteric viruses in pigs from five european countries. | enteric viral infections in pigs may cause diarrhea resulting in ill-thrift and substantial economic losses. this study reports the enteric infections with porcine astrovirus type 4 (pastv4), porcine group a rotavirus (garv), porcine group c rotavirus (gcrv), porcine circovirus type 2 (pcv2) and porcine kobuvirus (pkov) in 419 pigs, comprising both healthy and diarrheic animals, from 49 farms in five european countries (austria, germany, hungary, spain and sweden). real-time rt-pcr assays were d ... | 2016 | 26711031 |
| human bocavirus in hospitalized children with acute gastroenteritis in russia from 2010 to 2012. | human bocavirus (hbov) can cause respiratory diseases and is detectable in the stool samples of patients with gastroenteritis. to assess the prevalence of hbov in children hospitalized with acute gastroenteritis in novosibirsk, russia, as well as its genetic diversity and the potential role in the etiology of gastroenteritis in this region, a total of 5502 stool samples from children hospitalized with gastroenteritis from 2010 to 2012, n=5250, and healthy children, n=252, were assayed for the pr ... | 2016 | 26602159 |
| predominance of g9p[4] rotavirus from children with acute gastroenteritis in northwestern mexico. | in mexico, group a rotavirus (rva) infections remain the most common cause of severe dehydrating diarrhea in children. this study was conducted to examine the circulating rva strains in the northwest region of mexico. rva strains collected from stool samples of children were genotyped, and their partial sequences were analyzed. rt-pcr of the vp4 and vp7 genes showed the partial g9p[4] genotype in all the samples. sequencing and phylogenetic analysis of the partial vp7 gene amplicons of 10 strain ... | 2016 | 28334725 |
| outbreak of g2p[4] rotavirus gastroenteritis in a retirement community, brazil, 2015: an important public health risk? | the present study described a group a rotavirus (rva) outbreak in an age-care facility in brazil, using epidemiologic and molecular diagnostic methods. a descriptive clinical, epidemiological and environmental investigation was conducted. stool samples were collected and screened for rva, norovirus (nov), enteric adenovirus 40/41 (adv 40/41) and astrovirus (astv) using elisa, rt-pcr, qrt-pcr, electron microscopy and sequencing methods. outbreak occurred during 26th-29th october, 2015; 28 individ ... | 2016 | 27964971 |
| whole-genome characterization of a peruvian alpaca rotavirus isolate expressing a novel vp4 genotype. | the sa44 isolate of rotavirus a (rva) was identified from a neonatal peruvian alpaca presenting with diarrhea, and the full-length genome sequence of the isolate (designated rva/alpaca-tc/per/sa44/2014/g3p[40]) was determined. phylogenetic analyses showed that the isolate possessed the genotype constellation g3-p[40]-i8-r3-c3-m3-a9-n3-t3-e3-h6, which differs considerably from those of rva strains isolated from other species of the order artiodactyla. overall, the genetic constellation of the sa4 ... | 2016 | 27939152 |
| detection and whole-genome characterization of a g8p[1] group a rotavirus strain from deer. | rotavirus a strain 14-02218-2, with genome constellation g8p[1]-i2-r2-c2-m2-a3-n2-t6-e2-h3, was isolated from newborn fawns. the 14-02218-2 rotavirus strain is related to bovine and bovine-like rotavirus strains. to our knowledge, this is the first report on whole-genome-based characterization of a deer rotavirus g8p[1] strain. | 2016 | 27932644 |
| medicinal plants and natural molecules with in vitro and in vivo activity against rotavirus: a systematic review. | rotaviruses can cause life-threatening health disorders, such as severe dehydrating gastroenteritis and diarrhea in children. vaccination is the main preventive strategy to reduce rotavirus diarrhea and the severity of episodes, but vaccines are not fully effective and new episodes may occur, even in vaccinated children. the who recommends oral rehydration therapy and zinc supplementation for rotavirus-induced diarrhea management. there is little preclinical evidence to support the use of phytot ... | 2016 | 27912886 |
| detection of a novel equine-like g3 rotavirus associated with acute gastroenteritis in brazil. | genotype g3p[8] of rotavirus a (rva) is detected worldwide, usually associated with wa-like constellation and exhibiting a long rna migration pattern. more recently, a novel inter-genogroup, g3p[8] reassortant variant with a short electropherotype, has emerged in asia, oceania and europe, denoting an overall potential of unusual rotavirus strains. during a rva surveillance in brazil, g3p[8] strains were found displaying a short electropherotype pattern, which had not been detected before in this ... | 2016 | 27902376 |
| high genetic diversity of species a rotaviruses detected in swine farms in chile. | rotavirus a is one of the main causative agents of diarrhea in lactating and weaned pigs worldwide. its impact in the swine industry is well documented. however, in chile the current epidemiological status of rotavirus in porcine farms is unknown. this study evaluated the current epidemiologic status of rotavirus a infection in chile using on-farm detection technique, electrophoretic confirmation, genotyping and phylogenetic clustering by analysis of partial sequences of vp4 and vp7 genes. rotav ... | 2016 | 27902360 |
| from lab to lake - evaluation of current molecular methods for the detection of infectious enteric viruses in complex water matrices in an urban area. | quantitative pcr methods are commonly used to monitor enteric viruses in the aquatic environment because of their high sensitivity, short reaction times and relatively low operational cost. however, conclusions for public health drawn from results of such molecular techniques are limited due to their inability to determine viral infectivity. ethidium monoazide (ema) and propidium monoazide (pma) are capable to penetrate the damaged or compromised capsid of the inactivated viruses and bind to the ... | 2016 | 27880820 |
| detection and characterisation of rotaviruses from children less than 5 years hospitalised with acute gastroenteritis in nagercoil. | group a rotavirus continues to be the major cause of severe gastroenteritis in young children in developing countries. in this study, we report the prevalence and genotype of rotaviruses identified from children <5 years of age hospitalised with acute gastroenteritis from nagercoil, tamil nadu from 2007-2010. from the 139 children included in the study, 71 samples (51%) were positive by elisa and 65 samples were positive by pcr-based methods. g1p[8] (44.6%) was the most commonly identified genot ... | 2016 | 23508433 |
| analysis of a genotype g3p[9] rotavirus a strain that shows evidence of multiple reassortment events between animal and human rotaviruses. | the species a rotaviruses (rva) are important gastroenteric pathogens that infect humans and animals. rva genotype g3p[9] has been described in human-animal reassortment events, and the complexity of its hosts motivates the genetic investigation of this strain. therefore, the aim of this study is to analyse a g3p[9] sample that was detected in a child with acute gastroenteritis. the 1a3739 sample featured the constellation g3p[9]-i18-r3-c3-mx-a19-n3-t3-e3-h6. the sequence for vp3 gene was not ob ... | 2016 | 27862014 |
| first molecular detection and vp7 (g) genotyping of group a rotavirus by semi-nested rt-pcr from sewage in nigeria. | rotavirus is the leading cause of viral gastroenteritis worldwide, and sewage is a major source of the virus dissemination in the environment. our aim was to detect and genotype rotaviruses from sewages in nigeria. one hundred and ninety sewage samples were collected between june 2014 and january 2015. the two phase concentration method using peg 6000 and dextran was used to concentrate sewage samples following who protocols. molecular detection was performed by rt-pcr, and vp7 genotyping by sem ... | 2016 | 27828615 |
| distribution of rotavirus vp7 and vp4 genotypes circulating in tunisia from 2009 to 2014: emergence of the genotype g12. | group a rotavirus (rva) represents the most important aetiological agent of diarrhoea in children worldwide. from january 2009 to december 2014, a multi-centre study realized through 11 tunisian cities was undertaken among children aged <5 years consulting or hospitalized for acute gastroenteritis. a total of 1127 faecal samples were collected. all samples were screened by elisa for the presence of rva antigen. rva-positive samples were further analyzed by page and used for g/p-genotyping by sem ... | 2016 | 28118139 |
| dissemination of human adenoviruses and rotavirus species a on fomites of hospital pediatric units. | rotavirus a and human adenovirus dissemination were demonstrated both in a pediatric ward and in a neonatal intensive care unit (nicu) of the same pediatric hospital. virus detection from fomites samples were higher in the pediatric ward (42.3% [137 out of 324]) than in the nicu (4.5% [7 out of 156]), revealing that cleaning processes used in our nicu are effective in reducing viral contamination, suggesting human adenovirus as a potential biomarker of contamination of hospital fomites. | 2016 | 27217348 |
| predominance of unusual rotavirus g1p[6] strain in north india: an evidence from hospitalized children and adult diarrheal patients. | group a rotavirus remains the leading cause of gastroenteritis in children and accounts for 0.2 million fatalities each year; out of which, approximately 47,100 deaths occur in india. in adults also, rotavirus is reported to be responsible for diarrhea severe enough to require hospitalizations. india has recently introduced rotavirus vaccine in the universal immunization programme and himachal pradesh became the first indian state to implement this project. this study is an attempt to provide th ... | 2016 | 27806915 |
| antiviral activity of ergoferon against group a rotavirus. | antiviral activity of ergoferon was studied in vitro on an experimental model of rotavirus infection in ma-104 cell line. in infected cells treated with ergoferon, rotavirus titer was shown to decrease by 83 and 90% in comparison with cells treated with solvent used for ergoferon preparation (p<0.05) and distilled water (p<0.05), respectively. these findings demonstrate high anti-rotavirus activity of ergoferon. | 2016 | 27783292 |
| whole genomic analysis of bovine group a rotavirus strains a5-10 and a5-13 provides evidence for close evolutionary relationship with human rotaviruses. | bovine group a rotavirus (rva) is an important cause of acute diarrhea in calves worldwide. in order to obtain precise information on the origin and evolutionary dynamics of bovine rva strains, we determined and analyzed the complete nucleotide sequences of the whole genomes of six archival bovine rva strains; four thai strains (rva/cow-tc/tha/a5-10/1988/g8p[1], rva/cow-tc/tha/a5-13/1988/g8p[1], rva/cow-tc/tha/61a/1989/g10p[5], and rva/cow-tc/tha/a44/1989/g10p[11]), one american strain (rva/cow- ... | 2016 | 27771069 |
| genomic constellation and evolution of ghanaian g2p[4] rotavirus strains from a global perspective. | understanding of the genetic diversity and evolution of rotavirus a (rva) strains, a common cause of severe diarrhoea in children, needs to be based on the analysis at the whole genome level in the vaccine era. this study sequenced the whole genomes of six representative g2p[4] strains detected in ghana from 2008 to 2013, and analysed them phylogenetically with a global collection of g2p[4] strains and african non-g2p[4] ds-1-like strains. the genotype constellation of the study strains was g2-p ... | 2016 | 27569866 |
| [surveillance for viral diarrhea in sentinel hospitals in henan province, 2013-2015]. | objective: to investigate the infection status of human rotavirus, calicivirus, astrovirus and enteric adenovirus in children aged <5 years in disease surveillance areas in henan province from 2013 to 2015. methods: a total of 880 stool samples were collected from four sentinel hospitals and group a rotavirus was detected by elisa and group a rotavirus g/p genotyping was performed with nested multiplex rt-pcr, while rotavirus (group b, c), calicivirus and astrovirus were detected by two-step mul ... | 2016 | 27765132 |
| whole genomic analysis of g2p[4] human rotaviruses in mymensingh, north-central bangladesh. | rotavirus a (rva) is a dominant causative agent of acute gastroenteritis in children worldwide. g2p[4] is one of the most common genotypes among human rotavirus (hrv) strains, and has been persistently prevalent in south asia including bangladesh. in the present study, whole genome sequences of a total of 16 g2p[4] hrv strains (8 strains each in 2010 and 2013) detected in mymensingh, north-central bangladesh were determined. these strains had typical ds-1-like genotype constellation. most of gen ... | 2016 | 27722206 |