Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| rapid genotyping of human rotavirus using sybr green real-time reverse transcription-polymerase chain reaction with melting curve analysis. | to develop a real-time reverse transcription-polymerase chain reaction (rt-pcr) assay to genotype rotavirus (g and p) in alberta from january 2012 to june 2013. | 0 | 26568918 |
| group a rotavirus gastroenteritis: post-vaccine era, genotypes and zoonotic transmission. | abstractthis article provides a review of immunity, diagnosis, and clinical aspects of rotavirus disease. it also informs about the changes in epidemiology of diarrheal disease and genetic diversity of circulating group a rotavirus strains following the introduction of vaccines. group a rotavirus is the major pathogen causing gastroenteritis in animals. its segmented rna genome can lead to the emergence of new or unusual strains in human populations via interspecies transmission and/or reassortm ... | 0 | 27462899 |
| etiology of acute diarrhea in tunisian children with emphasis on diarrheagenic escherichia coli: prevalence and identification of e. coli virulence markers. | diarrheal diseases can be caused by viral, bacterial and parasitic infections. this paper provides a preliminary image of diarrhea with regards to etiology and epidemiologic factors in tunisian children less than five years of age. | 0 | 25909062 |
| [rotavirus: a frequent cause of acute gastroenteritis in children]. | 1977 | 189452 | |
| [acute gastroenteritis caused by rotavirus. a summary of danish studies]. | 1977 | 202050 | |
| rotavirus: a cause of nosocomial infection in the nursery. | 1982 | 6284903 | |
| rotavirus and hemolytic enteropathogenic escherichia coli in weanling diarrhea of pigs. | since the turn of the century, escherichia coli has been implicated in the etiology of weanling diarrhea (colibacillosis). however, rotavirus--a virus that destroys enterocytes--has been shown recently to be causally associated with weanling diarrhea of pigs. the role of both rotavirus and hemolytic enteropathogenic e. coli in weanling diarrhea was assessed in this study. pigs from a closed herd were farrowed and weaned by two markedly different systems: an "intensive care sanitary" system and a ... | 1982 | 6296193 |
| rapid detection of enteric adenovirus and rotavirus: a simple method using polyacrylamide gel electrophoresis. | a simple and rapid procedure for identifying adenovirus and rotavirus in stool extracts has been developed. the technique is based on polyacrylamide gel electrophoresis of the virus nucleic acid, but sample preparation is straightforward and does not entail phenol extraction or the use of a radioactive label. furthermore, processing is not influenced by the amount of specimen obtained and is thus suitable for application as a batch testing method. this approach removes the need for specific anti ... | 1984 | 6096404 |
| evidence that a novel rotavirus-like agent of rats can cause gastroenteritis in man. | a rotavirus-like agent known to cause gastroenteritis in rats was also found to be associated with diarrhoea in a human population. serological studies indicated that infection with this virus is prevalent in children and adults living in baltimore, maryland. the virus is antigenically and genomically distinct from group a rotaviruses and this should be borne in mind in efforts to develop vaccines for protection against infantile gastroenteritis. | 1985 | 2861512 |
| detection of rotaviruses by nucleic acid hybridization with cloned dna of simian rotavirus sa11 genes. | we developed a dot-blot hybridization assay to detect rotaviral rna sequences in tissue culture or in clinical samples. 32p-labeled cloned cdna probes of the simian rotavirus sa11 specifically detected rotaviral rna sequences and were more sensitive for detecting sa11 than was the commercial enzyme-linked immunosorbent assay rotazyme test. a full-length probe of sa11 gene 6 detected 2.5 x 10(5) sa11 particles or approximately 0.27 ng of purified sa11 dsrna. combined probes from genes 6 and 9 det ... | 1985 | 2993434 |
| etiologic studies of the 1983 and 1984 outbreaks of epidemic diarrhea in guangxi. | studies are reported on two outbreaks of epidemic diarrhea in china involving 19,007 patients. the first outbreak occurred in the northern part of the guangxi autonomous region and extended south from april through september 1983. in june 1984 a second outbreak of 6,570 cases occurred in guanyang, a county in the northern part of guangxi. 125 fecal samples from the two outbreaks were cultured for bacteria; all of the samples except one were negative. rotavirus particles were detected in 44.1% (1 ... | 1985 | 2999028 |
| replication of atypical ovine rotavirus in small intestine and cell culture. | colostrum-deprived lambs experimentally infected with an atypical ovine rotavirus isolated from naturally scouring animals, and a naturally infected colostrum-deprived lamb, were examined by immunofluorescence and immunoperoxidase labelling, and electron microscopy. a hyperimmune serum to the virus was produced in a gnotobiotic lamb and used to demonstrate antigen in the villous epithelial cells of the small intestine from infected animals. scanning and transmission electron microscopy of tissue ... | 1986 | 3005481 |
| genome analysis of human rotaviruses by oligonucleotide mapping of isolated rna segments. | the genomes of adult diarrhoea rotaviruses isolated in different parts of china during winter outbreaks in 1983 and 1984 were compared by segmental oligonucleotide (on) mapping. the rna profiles of most of the isolates were indistinguishable but it was found that some corresponding rna segments had identical or very closely related on maps whereas others differed considerably. this finding can be taken to suggest that the strains compared may be genetically related by a natural reassortment even ... | 1986 | 3014782 |
| isolation and serial propagation of turkey rotaviruses in a fetal rhesus monkey kidney (ma104) cell line. | turkey rotaviruses from the intestinal contents of poults were isolated and serially propagated in ma104 cell monolayers by a simple procedure. the initial virus isolation was done by low-speed centrifugation of the inoculum onto the monolayers, and subsequent passages were accomplished in roller-tube monolayers using trypsin-treated virus suspensions. each of the turkey rotavirus isolates possessed the morphologic, antigenic, and genomic attributes characteristic of turkey group a rotaviruses. ... | 1986 | 3015110 |
| identification of a bovine enteric syncytial virus as a nongroup a rotavirus. | an atypical or nongroup a rotavirus was identified in feces obtained from gnotobiotic calves in which fecal preparations originally derived during an epizootic of neonatal calf diarrhea had been serially passaged. the epizootic was previously reported to be caused by a noncharacterized viral agent that induced the formation of epithelial syncytia on small intestinal villi of experimentally infected calves. this bovine, nongroup a rotavirus was found to be antigenically related to a described aty ... | 1986 | 3021026 |
| incidence of group a and atypical rotaviruses in brazilian pig herds. | the incidence of rotaviruses as a gastroenteritis causal agent in piglets was studied in 19 pig herds of sao paulo state, brazil, during 1985. from 302 diarrhoea samples collected during january (summer), 65 were positive for rotavirus when analysed by enzyme-linked immunosorbent assay (elisa) and polyacrylamide gel electrophoresis (page). sixty-two of these samples belonged to the classical group a rotavirus, three to atypical rotaviruses (elisa negative and probably group b) and one elicited a ... | 1986 | 3022362 |
| characterisation of rotavirus isolates from sub-clinically infected calves by genome profile analysis. | rotaviruses isolated from 43 sub-clinically infected calves from a single farm were analysed by genome profile analysis. the isolates showed genomic variation and eight different profiles were observed, including one which was atypical for group a rotaviruses. the 3' terminal labelling method for the analysis of genome profiles used in this study required only 1 ng of viral rna, an increase of 1000-fold in sensitivity over ethidium bromide staining for detecting all rotavirus genome segments. ho ... | 1987 | 3027953 |
| physicochemical characterization of porcine pararotavirus and detection of virus and viral antibodies using cell culture immunofluorescence. | a cell culture immunofluorescence (ccif) assay was optimized for detection of porcine pararotavirus (group c rotavirus) in intestinal contents. the greatest viral infectivity was observed when ma104 cells (5 days after subculturing) were rinsed and refed in serum-free medium before inoculation, pancreatin was added to the inocula, and the inocula were centrifuged onto the cells. gentamicin treatment of pararotavirus samples to reduce bacterial contamination also reduced the viral infectivity of ... | 1987 | 3029161 |
| age-related infections with rotavirus, rotaviruslike virus, and atypical rotavirus in turkey flocks. | the genome electropherotyping technique was used in longitudinal surveys to detect group a rotavirus, rotaviruslike virus (rvlv), atypical rotavirus (atr), and reovirus in intestinal contents or fecal specimens collected from turkeys in 10 commercial and 2 research station flocks. these viruses were detected in turkeys from 8 to 10 commercial flocks surveyed. of 278 specimens collected from turkeys less then 29 days old in commercial flocks, 79 (28.4%) contained one or more viruses, whereas only ... | 1987 | 3029167 |
| isolation and characterization of two group a rotaviruses with unusual genome profiles. | analysis of the genomic dsrna of rotaviruses isolated from calves with subclinical infections has revealed eight calves excreting group a viruses with unusual genome profiles. two of these virus strains, c7-176 and c7-183, were grown and cloned by plaque purification in cell culture. examination of their genome profiles after cloning showed that the unusual pattern had been retained. they were without rna segment 11 but had an extra band (6a) migrating between segments 6 and 7. however, they con ... | 1987 | 3029296 |
| detection of antibody to group b adult diarrhea rotaviruses in humans. | group b rotaviruses have been responsible for annual epidemics of severe diarrhea affecting both adults and children in china. we developed a specific and sensitive enzyme-linked immunosorbent blocking assay to detect antibody to group b rotaviruses that will be useful to assess the role of group b rotavirus infections as a cause of human gastroenteritis. we tested 219 human sera and 18 immunoglobulin pools collected from eight countries for antibodies to both group a and group b rotaviruses. ov ... | 1987 | 3034964 |
| comparative pathology of infection by novel diarrhoea viruses. | examination of diarrhoeic faeces in the electron microscope often reveals viruses that are presumed to be enteropathogenic. lesions caused by novel rotaviruses were similar to those of group a rotaviruses, but enterocyte syncytia were seen which are probably pathognomonic for novel rotaviruses. in adenovirus infection in piglets, mature enterocytes were infected and destroyed; intranuclear inclusion bodies were seen in infected enterocytes. calici-like viruses infected mature enterocytes in calv ... | 1987 | 3036441 |
| nucleic acid-based analyses of non-group a rotaviruses. | simple genome profile studies on polyacrylamide gels allow all non-group a rotaviruses isolated so far to be recognized by the absence of the tight triplet (7-9) of rna segments seen in all group a viruses. however, reliance solely on genome profile studies for rotavirus grouping can be misleading and, for virus group definition, additional corroborating nucleic acid and serological studies are essential. terminal fingerprint analysis was the first generation of nucleic acid-based assays that al ... | 1987 | 3036444 |
| seroepidemiology and molecular epidemiology of the chinese rotavirus. | the chinese rotavirus which causes epidemics of diarrhoea in adult humans was isolated in 1983. this virus, designated adult diarrhoea rotavirus (adrv), resembles typical rotaviruses morphologically and has a genome made up of 11 discrete segments of double-stranded rna. because the chinese rotavirus has a unique rna pattern on polyacrylamide gel electrophoresis and is antigenically distinct from group a rotaviruses, it is tentatively included in group b. infection with adrv or adrv-related viru ... | 1987 | 3036445 |
| novel rotaviruses in animals and man. | novel (non-group a) rotaviruses have many of the morphological, biochemical and biological properties described originally for group a rotaviruses but they do not share the same group antigens. by negative-stain electron microscopy, novel rotaviruses have the characteristic rotavirus morphology, although with some novel rotaviruses the characteristic single- and double-shelled particles may not be readily apparent. comparison of novel rotaviruses in serological tests has revealed the existence o ... | 1987 | 3036446 |
| first report of an epidemic of diarrhoea in human neonates involving the new rotavirus and biological characteristics of the epidemic virus strain (kmb/r85). | an outbreak of diarrhoea in neonates occurred at the nurseries of the department of obstetrics of zhao tong regional hospital, yunnan province, from the middle of august to the end of november, 1985. fifty-one percent of children were affected 2-8 days after birth. the clinical symptoms were mild; patients mainly had diarrhoea and did not vomit. rotaviruses were detected in 66.7% by rna page and in 72.7% by em. the virus strain designated as kmb/r85 had a typical morphology, which was indistingu ... | 1987 | 3040899 |
| isolation of an atypical rotavirus causing diarrhea in neonatal ferrets. | a rotavirus was isolated from neonatal ferrets (mustela putorius furo) with diarrhea at a large commercial farm. this virus is classified as an atypical rotavirus, probably belonging to the group c rotaviruses. this classification is based on the lack of the rotavirus group a common antigen and on its distinct dsrna electropherotype pattern in polyacrylamide gels. the diarrheal disease was reproduced experimentally in neonatal ferrets. | 1987 | 3599884 |
| electron microscopy procedure influences detection of rotaviruses. | technical parameters of electron microscope staining procedures (type of stain, ph of stain, and time of staining) influence particle integrity for three groups of rotaviruses. simian rotavirus sa11 (group a), chinese adult diarrhea rotavirus and porcine rotavirus-like agent (group b), and porcine pararotavirus (group c) were tested. all rotavirus strains were quite stable in uranyl acetate and phosphotungstic acid at ph 4.5 and relatively stable in ammonium molybdate. however, staining with pho ... | 1987 | 2444622 |
| group a rotavirus excretion patterns in naturally infected pigs. | cross-sectional and cohort studies were conducted in a piggery to investigate the excretion pattern of group a rotavirus in pigs. the cross-sectional survey revealed that 47 (9 per cent) of 521 pigs sampled were excreting rotavirus in the faeces. no rotavirus antigen was detected in the faeces of pigs either less than one week or over two months old. the prevalence of infection increased with age over the sucking period, and was greatest at five weeks old. diarrhoea was observed in only eight (1 ... | 1987 | 2832911 |
| [heterologous system of immunoenzyme analysis for the diagnosis of human rotavirus infection and its comparison with rna electrophoresis]. | on the basis of porcine rotavirus a heterologous eia test system was worked out and tested for diagnosis of human rotavirus infection. a high sensitivity and specificity of the test system was demonstrated, its results were compared with those of electron microscopy, diffuse precipitation test, and rna electrophoresis. out of 201 specimens (fecal filtrates) collected from children ranging in ages from 14 days to 10 years, rotavirus antigen was detected in 68 (33.8%) rna electrophoresis demonstra ... | 1988 | 2835872 |
| partial characterization of a bovine group a rotavirus with a short genome electropherotype. | a group a rotavirus (id isolate) recovered from a diarrheic beef calf possessed a short genome electropherotype. this short genome electropherotype was a stable characteristic of the id isolate as it remained unchanged through 3 passages in gnotobiotic calves or through 19 passages in ma104 cell cultures. subgroup analysis with monoclonal antibodies in an enzyme-linked immunosorbent assay established that the isolate was a subgroup 1 rotavirus. neutralization tests demonstrated that this isolate ... | 1988 | 2838513 |
| isolation of two new serotypes of porcine rotavirus from pigs with diarrhea. | two group a rotaviruses (isu-64 and isu-65) with subgroup i antigen were isolated from pigs with diarrhea and were shown by two-way neutralization tests to be two new serotypes of porcine rotavirus. | 1988 | 2839130 |
| serial propagation of porcine group c rotavirus (pararotavirus) in a continuous cell line and characterization of the passaged virus. | the cowden strain of porcine group c rotavirus (pararotavirus) was adapted to serial passage in a continuous monkey kidney cell line (ma104). key factors in its successful adaptation included use of virus passaged in primary porcine kidney cells as the initial inoculum, use of roller tubes, and addition of pancreatin to the maintenance medium. a cell culture immunofluorescence test was used to quantitate the virus at each passage level, since a possible cytopathic effect was obscured by the effe ... | 1988 | 2842368 |
| use of polyclonal and monoclonal antibodies and analysis of viral rna in the detection of unusual group a human rotaviruses. | immuno-enzymatic assay employing monoclonal antibodies and viral rna analysis by gel electrophoresis were used to classify human group a rotaviruses (hrv) into subgroups i and ii. of 249 fecal samples positive for group a rotaviruses, 29 (11%) belonged to subgroup i and 215 (85%) were identified as subgroup ii. two samples (ro-302 and ro-500) contained mixed infections of the two subgroups. three isolates belonged to neither one of the two subgroups, but they did not yield enough viral rna to al ... | 1988 | 2844982 |
| rotavirus epidemiology in vellore, south india: group, subgroup, serotype, and electrophoretype. | rotaviruses were detected in 163 of 916 (17.8%) specimens collected from children under 3 years of age with gastroenteritis in vellore, south india, between august 1983 and july 1985. rotaviruses were detected throughout the study period, with a peak prevalence in december to february (winter) and june to august (southwest monsoon season). a total of 117 rotavirus strains were tested for subgroup, serotype, and rotavirus double-stranded rna electrophoretic migration pattern; 24.8% of the strains ... | 1988 | 2853177 |
| serological evidence of rotavirus infection in a guinea pig colony. | antibodies reacting with simian rotavirus sa11 were detected by enzyme immunoassay (eia) and western blot assay (wba) in sera from guinea pigs bred for experimental use at the fundação oswaldo cruz, rio de janeiro, brazil. the proportion of antibody-positive animals and the antibody titres rose sharply in 1985, were maintained at a high levels in 1986 and declined in 1987. there were no obvious signs of disease coinciding with serological evidence of infection. results of wba suggest that the vi ... | 1988 | 2856077 |
| identification of cross-reactive and serotype 2-specific neutralization epitopes on vp3 of human rotavirus. | the group a rotaviruses are composed of at least seven serotypes. serotype specificity is defined mainly by an outer capsid protein, vp7. in contrast, the other surface protein, vp3 (775 amino acids), appears to be associated with both serotype-specific and heterotypic immunity. to identify the cross-reactive and serotype-specific neutralization epitopes on vp3 of human rotavirus, we sequenced the vp3 gene of antigenic mutants resistant to each of seven anti-vp3 neutralizing monoclonal antibodie ... | 1988 | 2453680 |
| identification of a group-reactive epitope of group b rotaviruses recognized by monoclonal antibody and application to the development of a sensitive immunoassay for viral characterization. | group b rotaviruses (gbrs) are fastidious agents which cause enteric disease in humans and a number of other animal species. detailed study of the role of gbrs in human disease has been hampered by the lack of immunoreagents suitable for large-scale studies. we developed a monoclonal antibody which recognizes a group-reactive antigen contained in a number of strains of gbrs. when utilized in conjunction with a hyperimmune guinea pig antiserum to gbr, this monoclonal antibody can be used in an en ... | 1988 | 2460499 |
| [molecular epidemiology of rotaviruses]. | group a rotaviruses have been recognized worldwide as the main viral cause of acute infantile gastroenteritis and of diarrhoea of the young of a wide variety of animal species. the molecular epidemiology of rotaviruses has revealed an extensive genomic and antigenic variability of successively occurring or cocirculating human group a rotaviruses. group b rotaviruses have been found to cause outbreaks of acute gastroenteritis among adults in china. the genomic and antigenic diversity of human rot ... | 1988 | 2463221 |
| kinetics of intestinal replication of group b rotavirus and relevance to diagnostic methods. | non-group-a rotaviruses have been implicated with increasing frequency as causes of acute gastroenteritis in humans and other animals. however, the incidence and significance of infection with these agents, as well as appropriate diagnostic strategies for making these determinations, are largely unknown. studies to make these determinations could be more accurately conducted if the relationship between the viral replication kinetics and the particular diagnostic method used is understood. we thu ... | 1988 | 2830307 |
| non-group a rotavirus infection associated with severe gastroenteritis in a bone marrow transplant patient. | 1988 | 2830587 | |
| detection of human calicivirus antigen and antibody by enzyme-linked immunosorbent assays. | enzyme-linked immunosorbent assays (elisas) were developed to detect human calicivirus (hcv) antigen and antibody to hcv. the elisas were specific for hcv and as sensitive as a previously developed radioimmunoassay. these elisas were used to search for evidence of hcv infection in the united states, where hcv gastroenteritis has rarely been reported. one hundred sixty-three stool samples collected from children hospitalized with diarrhea were examined; one sample was positive in the elisa. typic ... | 1988 | 3182991 |
| molecular epidemiology and subgroup determination of bovine group a rotaviruses associated with diarrhea in dairy and beef calves. | the genome electropherotyping technique was used to examine group a rotaviral infections of diarrheic calves ranging from 1 to 85 days of age in 2 beef and 27 dairy herds. coelectrophoresis studies demonstrated 38 distinct bovine group a rotavirus genome electropherotypes; all were long genome electropherotypes, and none had extra segments or unusual segment rearrangements. genome electropherotypes in fecal specimens from diarrheic calves previously inoculated orally with a commercial, modified- ... | 1989 | 2463994 |
| preparation and characterization of monoclonal antibodies to rotavirus. | by utilizing a strain of cultivable simian rotavirus (sa-11) as an immunizing antigen, we prepared 4 clones of mouse-mouse hybridoma, namely c127, c139, c172, and c214 which secreted monoclonal antibodies against the immunogen itself, sa-11 and also against other group a strains such as wa and s2. western blot analyses revealed that all of these antibodies are directed against vp6, a 42 kda major inner capsid protein of group a rotavirus. competitive experiments suggested that c127, c172 and c21 ... | 1989 | 2469693 |
| temporal variation of malaysian rotavirus electropherotypes. | an analysis of rotavirus electropherotypes circulating in kuala lumpur, malaysia, over 7 years showed that all except 1 of the 360 electropherotypes encountered were characteristic of group a rotaviruses. the long electropherotype predominated annually, and there was a rarity of short electropherotypes. extensive genome variability and cocirculation of different electropherotypes were observed annually. a sequential appearance of the predominant electropherotype was observed in all years of the ... | 1989 | 2470775 |
| seroepidemiology of adult diarrhea rotavirus in china, 1977 to 1987. | in 1982, large outbreaks of diarrhea that were caused by group b adult diarrhea rotavirus (adrv) occurred throughout the people's republic of china. until 1982, group b rotavirus had never been associated with disease in humans. to determine whether adrv was a new virus introduced in 1982 or had been present before that time, we examined antibody titers of adrv in gamma globulin (pooled immunoglobulin) pools that were prepared during 1977 to 1987 in four cities in the people's republic of china ... | 1989 | 2479654 |
| nonreactivity of american avian group a rotaviruses with subgroup-specific monoclonal antibodies. | nine group a rotavirus isolates recovered from commercially reared poultry in the united states did not react with subgroup 1 (255/60) or subgroup 2 (631/9) specific monoclonal antibodies in an enzyme-linked immunosorbent assay. | 1989 | 2480363 |
| monoclonal antibody assay for detection of double-stranded rna and application for detection of group a and non-group a rotaviruses. | fastidious viruses are generally detected in human body fluids by means of immunoassay or nucleic acid hybridization systems. these approaches can be difficult to apply to the detection of viruses which display variations in antigenic or genetic composition. rotaviruses are examples of viruses which can display such variations. recently identified antigenic variants, designated as non-group a rotaviruses, cannot be detected by immunoassays or nucleic acid hybridization assays which utilize reage ... | 1989 | 2536393 |
| purification and characterization of adult diarrhea rotavirus: identification of viral structural proteins. | adult diarrhea rotavirus (adrv) is a newly identified strain of noncultivable human group b rotavirus that has been epidemic in the people's republic of china since 1982. we have used sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western (immuno-) blot analysis to examine the viral proteins present in the outer and inner capsids of adrv and compared these with the proteins of a group a rotavirus, sa11. edta treatment of double-shelled virions removed the outer capsid and resulted ... | 1989 | 2539512 |
| detection of group b rotavirus in fecal specimens by dot hybridization with a cloned cdna probe. | cloned cdna copies were synthesized from the genomic rna of the idir strain of group b rotavirus (gbr) isolated in baltimore, md. these clones were screened for hybridization with heterologous gbr to identify cdna for use in dot hybridization experiments. in multiple screening experiments, cdna clones derived from gene segment 3 provided the most intense hybridization signals. 32p-labeled probes were produced from one of the gene 3 clones, and these were employed in dot hybridization assays. pur ... | 1989 | 2541164 |
| experience with an enzyme immunoassay for serotyping human group a rotaviruses. | an enzyme immunoassay utilizing neutralizing monoclonal antibodies to vp7 of four human group a rotavirus serotypes successfully typed rotaviruses in 71.4% (568 of 796) of fecal specimens. sensitivity was enhanced by using homologous capture and detector antibodies. serotyping was most successful with specimens stored for less than 3 years and containing 10(4) or more particles per ml. | 1989 | 2541171 |
| evidence for a new rotavirus subgroup in india. | monoclonal antibodies specific for rotavirus subgroup 1 (sg1) and subgroup 2 (sg2) were used to analyse by enzyme immunoassay (eia) the subgroups of human rotavirus isolates obtained from three different parts of india during the period september 1985 to july 1987. we identified one isolate which failed to react with either sg1 or sg2 specific monoclonal antibodies, although it reacted well with a monoclonal antibody specific for group a rotaviruses. this finding suggests that it belongs to a ne ... | 1989 | 2544441 |
| natural transmission of group a rotavirus within a pig population. | five litters of piglets born within two days of each other, together with their dams, were investigated for faecal excretion of group a rotavirus antigen from birth to two months old. all the 50 piglets in these litters became infected with the virus between 19 and 35 days old. rotavirus excretion was first seen in one litter which was housed with other litters not included in this study. two days later, piglets of the second litter in another farrowing room began to excrete rotavirus, and then ... | 1989 | 2544970 |
| infection by enteric adenoviruses, rotaviruses, and other agents in a rural african environment. | from february 1985 to january 1986, 432 stool samples, 310 from rural african children with diarrhea and 122 from controls, were analysed for the presence of enteric viruses known to be associated with diarrhea. group a rotavirus elisa indicated 12.9% positivity among patients and 2.5% positivity among controls. only 23 of the 43 rotavirus elisa-positive stools were also positive by electron microscopy. nine children, three of whom were controls, were found to be shedding coronavirus-like partic ... | 1989 | 2547021 |
| rapid detection of human rotavirus strains in stools by single-sandwich enzyme-linked immunosorbent assay systems using monoclonal antibodies. | using murine monoclonal antibodies (mabs) raised against the common antigen of group a rotavirus (rv), two single-sandwich elisa systems were developed for detection of rv in stools: one using polyclonal antibody (pab) as capture and a mab as detector antibody (referred to as pab-mab assay); and the other based on the use of two different mabs as capture and detector antibodies (referred to as mab-mab assay). in each single-sandwich elisa system, samples and peroxidase-labeled mab were incubated ... | 1989 | 2547818 |
| comparative virulence of two porcine group-a rotavirus isolates in gnotobiotic pigs. | the virulence of 2 porcine group-a rotavirus isolates was compared. forty hysterotomy-derived 3-day-old gnotobiotic pigs were inoculated orally with 2 ml of intestinal homogenate containing either the ohio state university (osu) or the south dakota state university (sdsu) strain of porcine rotavirus or were inoculated with medium only. clinical signs of disease, body weight, distribution of viral antigen, fecal excretion of virus, and histologic lesions (observed by light and scanning electron m ... | 1989 | 2548420 |
| characterization by enzyme-linked immunosorbent assay using subgroup- and serotype-specific monoclonal antibodies of human rotavirus obtained from diarrheic patients in bangladesh. | by enzyme-linked immunosorbent assay with group a-, subgroup-, and serotype-specific monoclonal antibodies (mabs), we tested 414 stool specimens collected from pediatric and adult patients hospitalized with acute gastroenteritis between january and june 1988. of 414 specimens tested, 124 (30%) were positive for group a rotavirus. the subgroup was determined in 110 specimens (88.7%); 16.1% were subgroup i, and 72.6% were subgroup ii. two specimens reacted with both subgroup i- and subgroup ii-spe ... | 1989 | 2549093 |
| studies on rotavirus homologous and heterologous active immunity in infant mice. | homologous and heterologous active immunity was studied in mice with mammalian group a rotaviruses. one day old mice were vaccinated with one of the following rotaviruses: bovine b641 (serotype 6), bovine b223 (untyped), simian sa11 (serotype 3) and murine edim (untyped). at 10 days of age they were challenged with edim virulent virus or sa11 virus. all the vaccines induced a serological antibody response in the mice but only the homologous immune response was protective. | 1989 | 2550022 |
| subgroup and serotype distributions of human, bovine, and porcine rotavirus in thailand. | the subgroup and serotype specificities of human, bovine, and porcine group a rotaviruses in stool specimens collected in thailand were examined by an enzyme-linked immunosorbent assay by using subgroup- and serotype-specific monoclonal antibodies. a clear yearly change was observed in the serotype distribution of human rotavirus. between 1983 and 1984, serotype 4 was the most prevalent, while the highest frequency of serotype 2 was found between 1987 and 1988. all the bovine and porcine rotavir ... | 1989 | 2550515 |
| prospective study of community-acquired rotavirus infection. | we determined titers of group a rotavirus common antibodies and neutralizing antibodies against serotypes 1 to 4 of prototype human rotavirus (hrv) in cord blood and serum specimens obtained from 38 infants at 4-month intervals from birth until 2 years of age. nineteen of the infants developed one episode of hrv diarrhea each, and they were matched by age and birth weight with the other 19 infants, who did not develop hrv diarrhea during the follow-up period. we estimated the incidence rate of h ... | 1989 | 2550518 |
| serological characterization of bovine rotaviruses isolated from dairy and beef herds in argentina. | bovine rotaviruses isolated from beef and dairy herds in argentina were serotyped by the immunoperoxidase focus reduction assay as previously described (g. gerna, m. battaglia, g. milenesi, n. passarani, e. percivalle, and e. cattaneo, infect. immun. 43:722-729, 1984). three strains from beef herds were related to the uk and ncdv bovine rotavirus strains defined as serotype 6 (y. hoshino, r. g. wyatt, h. b. greenberg, j. flores, and a. z. kapikian, j. infect. dis. 149:694-702, 1984). two other s ... | 1989 | 2553769 |
| rapid screening test for the diagnosis of rotavirus infection. | the early diagnosis of human rotavirus infection is essential for effective patient management and infection control. we report here a rapid, easy-to-perform, and inexpensive test for rotavirus detection. the viral rna is extracted directly from the stools and electrophoresed on 1% agarose gels. currently available immunoassays for routine diagnostic purposes are directed at the common group a-specific antigen. as reports become available on human gastroenteritis caused by the atypical or novel ... | 1989 | 2555394 |
| temporal and geographical distributions of human rotavirus serotypes, 1983 to 1988. | between 1983 and 1988, subgroups and serotypes were determined for 907 of 1,084 clinical specimens of rotaviruses collected in various countries of europe, north and south america, africa, and asia. enhanced enzyme immunoassays based on monoclonal antibodies specific for rotavirus proteins vp6 and vp7 were used. significant differences in the prevalent serotypes were detected from year to year in the united kingdom and brazil and also in different countries during the same year. throughout the s ... | 1989 | 2556435 |
| detection and survival of group a rotavirus in a piggery. | samples of dust, faeces and effluent were collected from a piggery and examined for group a rotavirus, using a commercial elisa test, electron microscopy and inoculation of ma-104 cells. rotavirus antigen was demonstrated in samples collected from farrowing and weaner rooms but not from fattener and sow houses. rotavirus antigen was also detected in samples collected from a weaner room which had been free of piglets for three months. a cytopathic porcine rotavirus (british isolate sw20/21) was k ... | 1989 | 2557702 |
| studies on attenuation of rotavirus. a comparison in piglets between virulent virus and its attenuated derivative. | the development of rotavirus vaccines against acute gastroenteritis for human infants has been accorded a very high priority. several vaccine candidates all of which are live cultivated strains of animal origin have been tested in humans. however the nature of attenuation of these viruses for humans is unknown. in this study we have attenuated a pig rotavirus by 15 sequential passages in cell culture after which the virus no longer causes diarrhoea in piglets. the pathogenesis of infection of th ... | 1989 | 2558635 |
| presence of group a and non-a rotaviruses in neonatal piglets in campinas, sp, brazil. | rotaviruses were detected by polyacrylamide gel electrophoresis (page) in 11 (84.6%) of 13 faecal specimens from neonatal piglets with acute diarrhoea in a piggery near the city of campinas, state of são paulo, brazil. an immunoenzimatic assay for group a rotavirus (iea-a) was positive in ten of the samples, all of which showed a page profile typical of that group. another sample was showed a group b profile in page. an immunoenzimatic assay specific for group b (iea-b) for this faecal sample wa ... | 1989 | 2559308 |
| epidemiology of typical and atypical rotavirus infections in new zealand pigs. | polyacrylamide gel electrophoresis (page) and enzyme-linked immunosorbent assay (elisa) were employed to investigate the epidemiology of typical and atypical rotavirus infections in five piggeries. of 152 faecal samples examined, 46 (30 per cent) were positive by elisa for group a rotavirus. rotaviruses with electrophoretic patterns resembling groups a, b and c were detected. at least two and up to five different rotavirus electrophoretypes (typical and/or atypical) were detected in each of the ... | 1989 | 16031535 |
| a survey of specific pathogen-free chicken flocks for antibodies to chicken anaemia agent, avian nephritis virus and group a rotavirus. | sera originating from specific pathogen-free flocks from europe, north america and australia were examined by indirect immuno-fluorescence for antibodies to chicken anaemia agent, avian nephritis virus and group a rotavirus. some flocks were free of infection, but others showed evidence of infection with one, two or all of these viruses. in view of the pathogenic potential of these viruses and the possibility that they may be egg-transmitted, it is recommended that their elimination from specifi ... | 1989 | 18679854 |
| monoclonal antibodies to the vp6 of porcine subgroup i rotaviruses reactive with subgroup i and non-subgroup i non-subgroup ii strains. | a panel of 10 monoclonal antibodies produced after immunization with two porcine subgroup i rotavirus strains (osu and a46), and directed against the major inner capsid protein (vp6), fell into six patterns of reactivity when tested against a collection of human and animal group a rotavirus strains. monoclonal antibodies of pattern i recognized all rotavirus strains. antibodies of patterns 2 and 3 recognized all subgroup ii strains and some, but not all, subgroup i strains. pattern 4 antibodies ... | 1990 | 1693668 |
| subgroup classification of porcine group-a rotaviruses, using monoclonal antibodies in an enzyme-linked immunosorbent assay. | fifty-six samples of feces and intestinal contents from nonvaccinated diarrheal pigs with rotavirus infections were tested, using a subgroup (sgp)-specific elisa, to determine rotavirus sgp classification. forty-one percent (23/56) were sgp 1, 25% (14/56) were sgp 2, and 34% (19/56) were not classifiable. for classifiable samples, the geographic distribution for sgp 1 and sgp 2, respectively was: 60%/40% from ohio (n = 15), 63%/37% from other midwestern states (iowa, minnesota, nebraska, south d ... | 1990 | 1695069 |
| removal of inhibitory substances from human fecal specimens for detection of group a rotaviruses by reverse transcriptase and polymerase chain reactions. | a method was developed for the purification of rotavirus rna from fecal extracts in order to permit the sensitive identification of group a rotavirus in fecal specimens by the polymerase chain reaction. sequential reactions with reverse transcriptase and taq polymerase with directed primers from rotavirus gene 6 yielded characteristic 259-base-pair fragments that were then visualized by silver stain on a polyacrylamide gel. as few as 500 genomic copies of purified rotavirus rna could be detected ... | 1990 | 1696283 |
| [adult diarrhea rotavirus in jilin, china]. | in may 1986, an outbreak of epidemic acute diarrhea occurred in one city and three counties in yanbian area. the diarrheal cases were seen in all age groups, the majority of the cases were seen in adult age group. no bacterial pathogens were isolated in 22 fecal samples examined, however rotavirus like particles of 52-68 nm in diameter were found in 50% (11/22) of fecal samples by the immunoelectron microscopy using convalescent sera. examination by the adult diarrhea rotavirus (adrv) elisa kit ... | 1990 | 1962974 |
| [rotavirus etiology of hospital cases of acute gastroenteritis]. | the etiology of enteric illnesses occurring during hospitalization in children admitted in january-may, 1985, for respiratory tract illnesses was studied by several methods including electron microscopy (em), enzyme immunoassay (eia), and pag electrophoresis. rotaviruses were detected in 22 (44.9%) t of 49 children with symptoms of intestinal infection, in february in 11 (84.6%) out of 13 patients. the analysis of virus genome rna of 7 isolates positive in em and eia revealed in all the isolates ... | 1990 | 1964515 |
| comparison of three bovine group a rotaviruses possessing supershot genome electropherotypes. | 1990 | 1965588 | |
| relative prevalence of typical and atypical strains among rotaviruses from diarrheic pigs in conventional swine herds. | polyacrylamide gel electrophoresis was conducted on genomic rna extracted from rotaviruses detected in diarrheic pigs from conventional swine herds. ninety samples contained sufficient virus for rna band visualization and genome classification. genome profiles were characteristic of typical group a rotaviruses in 67.8% of the 90 samples, of group b rotaviruses in 10.0%, and of group c rotaviruses in 11.1%. in 11.1% of the samples, the presence of more than 11 bands suggested concurrent infection ... | 1990 | 1965637 |
| group a rotavirus infection in animals from an animal house and in wild-caught monkeys. | randomly selected samples from different animal colonies from two laboratory animal houses and from the wild-caught monkeys were tested for the presence of anti-rotavirus antibodies to estimate the rates of infection with group a rotavirus. antibodies to the common group a rotaviral antigen were detected by a competitive enzyme-linked immunosorbent assay (elisa) using reagents of who elisa rotavirus detection kit. the results of the study showed that white mice, albino rats, and guinea pigs from ... | 1990 | 2122998 |
| cdna cloning of each genomic segment of the group b rotavirus adrv: molecular characterization of the 11th rna segment. | the group b noncultivatable rotavirus, adrv, was purified from infected stool specimens. double-stranded rna was extracted, polyadenylated, reverse transcribed into cdna, and cloned into plasmid vector pat153. each cdna clone hybridized to a single adrv rna segment and cdna clones of each genomic rna segment were identified. sequencing of genomic rna and a full-length cdna clone (ad63) determined that adrv rna segment 11 is 631 bases in length and contains a single open reading frame of 170 amin ... | 1990 | 2158190 |
| rotaviral enteritis in a raccoon. | a hand-reared raccoon (procyon lotor) kit had severe diarrhea and died within 24 hr. gross and histopathologic findings were compatible with a diagnosis of viral enteritis. the immunoperoxidase test revealed rotavirus group a antigen in the intestinal mucosa. this is the first record of rotaviral enteritis in a raccoon. | 1990 | 2160022 |
| group- and type-specific serologic response in infants and children with primary rotavirus infections and gastroenteritis caused by a strain of known serotype. | antibody response to group a rotavirus (rv), investigated in paired sera from 72 infants and young children with acute gastroenteritis caused by an rv infection, was diagnosed on the basis of a fourfold or greater rise in group a common rv igg antibody titer. virus-specific igm was detected in sera from 64 patients showing seroconversion; these were considered primary infection. rv was detected in stools of 56 (77.8%) patients with serologic evidence of infection and 54 were considered primary i ... | 1990 | 2161039 |
| biochemical characterization of the structural and nonstructural polypeptides of a porcine group c rotavirus. | purified virions or radiolabeled lysates of infected ma104 cells were used to characterize the structural and nonstructural polypeptides of a porcine group c rotavirus. at least six structural proteins were identified from purified group c rotavirus by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. of these, two (37,000- and 33,000-molecular-weight polypeptides) were associated with the outer shell, as demonstrated by the ability of edta to remove them from the purified virion. the o ... | 1990 | 2161936 |
| serotyping of human group a rotavirus with oligonucleotide probes. | rotaviruses (rv) in stools of children with diarrhea in thailand were serotyped by monoclonal enzyme immunoassay (meia), and rna extracted from these specimens were tested for hybridization with oligonucleotides constructed from the nucleotide sequences of vp7 of human serotypes 1, 2, 3, and 4. of 178 specimens that contained rv as identified with a monoclonal antibody to group a rv, 84% (149/178) hybridized with serotype-specific oligonucleotides, and 42% (74/178) were serotyped by meia (p less ... | 1990 | 2165109 |
| serotype variation of human group a rotaviruses in two regions of the usa. | the first longitudinal study of group a rotavirus serotype distribution in the usa is reported. elisas incorporating neutralizing monoclonal antibodies specific for the vp7 protein of serotypes 1, 2, 3, and 4 were used to determine the antigenic variation of group a rotaviruses in two collections of stool specimens. stool samples were collected from children hospitalized during 1979-1989 at texas children's hospital, houston, and from children from a more rural population hospitalized during 198 ... | 1990 | 2167332 |
| identification of the gene encoding the group b rotavirus vp7 equivalent: primary characterization of the adrv segment 9 rna. | gene segment 9 of the adult diarrheal rotavirus, adrv, has been cloned, and the full-length gene 9 cdna has been sequenced. sequences at the 5' and 3' termini of the gene 9 double-stranded rna were confirmed by direct rna sequencing. the ninth rna segment is 814 bases in length with a single open reading frame beginning at base 10 and terminating at base 757. the encoded polypeptide contains 249 amino acids with a calculated molecular weight of 28.5 kda. the protein contains three potential n-li ... | 1990 | 2167559 |
| molecular epidemiology of rotavirus in children attending day care centers in houston. | serotype distribution of group a rotavirus in children attending day care centers has not been previously reported. fifteen centers in houston participated in longitudinal monitoring of rotavirus infection during one to three consecutive rotavirus seasons. monitoring included daily recording of the presence of diarrhea and weekly stool collection. stool specimens containing rotavirus were analyzed by a monoclonal antibody (mab)-based elisa specific for the vp7 protein of serotypes 1, 2, 3, and 4 ... | 1990 | 2169496 |
| growth of group a rotaviruses in a human liver cell line. | recent observations in children with rotavirus gastroenteritis and in infant mice given rotavirus vaccine by oral administration suggest that this well-known gastrointestinal pathogen may infect the liver. to examine this possibility, the susceptibility of hep g2 cells to infection with a variety of rotavirus strains was tested. these cells were used because they are considered to be well differentiated and exhibit many liver-specific functions. the hep g2 cells supported the growth of the simia ... | 1990 | 2170264 |
| sequence analysis of the gene (6) encoding the major capsid protein (vp6) of group c rotavirus: higher than expected homology to the corresponding protein from group a virus. | two overlapping c-dna clones, which hybridized in northern blots to rna segment 6 of the prototype strain (cowden) of group c rotavirus, were selected from a c-dna library in pbr322 and sequenced. the gene 6 sequence obtained was 1349 nucleotides and contained a single long open reading frame encoding a protein of 394 amino acids (total mw, 44,479) which is in line with the size of the major capsid protein vp6. comparison of the group c sequence with that of the corresponding group a rotavirus g ... | 1990 | 2171196 |
| serotypic differentiation of group a rotaviruses with porcine rotavirus gene 9 probes. | the serotypic specificities of gottfried and osu porcine rotavirus gene 9 probes were investigated in a dot hybridization assay. the probes were reacted with homologous and heterologous serotypes of group a rotaviruses of human and animal origin. hybridizations were conducted under relatively low-stringency (52 degrees c, no formamide, 5 x ssc) and high-stringency (52 degrees c, 50% formamide, formamide, 5 x ssc) conditions (1 x ssc is 0.15 m nacl plus 0.015 m sodium citrate). under conditions o ... | 1990 | 2174902 |
| [epidemiological study of group c rotavirus]. | by electron microscopy survey of acute gastroenteritis of children in matsuyama, rotaviruses were detected in 561 of 2479 fecal samples obtained between october, 1984 and september, 1988, in which 60 atypical and 259 typical rotaviruses, so far tested, were detected by polyacrylamide gel electrophoresis (page) of viral rna. out of 60 atypical rotaviruses, 5 were observed in 1985, 7 in 1986, none in 1987 and 48 in 1988. these atypical rotaviruses were morphologically indistinguishable from typica ... | 1990 | 2258643 |
| transfer of maternal antibody against group a rotavirus from sows to piglets and serological responses following natural infection. | an enzyme-linked immunosorbent assay (elisa) was developed for the measurement of antirotaviral antibody in sera and faeces from pigs and used to study the dynamics of antirotaviral antibody responses in three cohorts of pigs. piglets acquired antirotaviral antibody by sucking their dams soon after birth. antirotaviral antibodies of iga and igg classes were detected in both colostrum and milk of all sows tested but igm class antibodies were not. the antibody levels in colostrum were eight to 32 ... | 1990 | 2359885 |
| detection of group b and c rotaviruses by polymerase chain reaction. | we adapted the polymerase chain reaction (pcr) to detect the noncultivatable group b and c rotaviruses and introduced a simple and convenient technique to purify viral rna from stool specimens. double-stranded rna present in stool extracts was purified by adsorption to hydroxyapatite and was used as the template for reverse transcription and polymerase amplification. primer pairs specific for group b (gene 8) and group c (gene 6) rotaviruses were selected to amplify group-characteristic sizes of ... | 1991 | 1645368 |
| analysis of serotypes and electropherotypes of equine rotaviruses isolated in the united states. | equine group a rotaviruses isolated over a 10-year period in new york state, new jersey, kentucky, and texas were compared serotypically and electropherotypically. all isolates were determined to be serotype 3 by reaction with hyperimmune antiserum to the serotype 3 h-2 strain of equine rotavirus. all displayed rna electrophoretic migration patterns related to that of the h-2 strain but distinct from that of serotype 5 strain h-1. a serologic survey of 184 mares in kentucky, which was done to de ... | 1991 | 1647407 |
| identification and partial characterization of a rhesus rotavirus binding glycoprotein on murine enterocytes. | in order to assess the possibility that rotavirus binds to a specific cellular receptor on enterocytes, we have used a viral overlay protein blot assay to study viral binding to murine intestinal brush border membranes (bbm). infectious double-shelled particles of rhesus rotavirus bound specifically to two approximately 300- and 330-kda glycoproteins from bbm prepared from suckling mice. significantly less rotavirus binding was observed when adult bbm were examined. rats have never been shown to ... | 1991 | 1649504 |
| epidemiology of group c rotavirus gastroenteritis in matsuyama, japan. | the epidemiology of rotavirus gastroenteritis was studied in the matsuyama district from january to may 1988. among strains of rotaviruses detected 46 showed the group c-specific page pattern of virus rna. group c rotavirus gastroenteritis occurred following an epidemic of group a rotavirus infection, primarily in children aged 4-7 years but rarely in those aged 0-1 years. this was seriologically confirmed after the epidemic. unlike the previous strains detected in matsuyama in 1985-1986, page o ... | 1991 | 1649541 |
| rotavirus infection detected in neonates from hospitals in urban bangladesh. | a study of neonatal group a rotavirus (rv) infection in 3 hospitals of urban bangladesh identified 60 infected babies of 100 studied in 2 hospitals. preliminary evidence based on serotyping and electropherotyping suggest that these rvs differ from community strains. | 1991 | 1650551 |
| identification and characterization of a plaque forming avian rotavirus isolated from a wild bird in japan. | from fresh faeces of a wild bird (melanitta fusca), a virus that showed granular cytopathic effects (cpe) on chicken kidney cell (ckc) cultures was isolated. by indirect immunofluorescence analyses (ifa), this isolate reacted with an antiserum against a bovine rotavirus. the isolate produced clear plaques on ckc by conventional techniques, without trypsin. three virus plaques were selected by plaque size (small, medium, and large) and cloned by three successive plaque cloning. in the sds-page an ... | 1991 | 1653040 |
| rotavirus-associated diarrhea in rural bangladesh: two-year study of incidence and serotype distribution. | stools were evaluated from 5,811 patient visits for treatment of diarrhea in matlab, bangladesh, between june 1987 and may 1989. the stools were analyzed to determine the distribution of serotypes of group a rotaviruses (rv). a total of 898 stool samples (15.5%) contained rv, as determined by using an enzyme-linked immunosorbent assay. rv isolates from 855 of these samples were serotyped by using serotype-specific synthetic oligonucleotide probes. a total of 558 (65.3%) could be assigned to spec ... | 1991 | 1653263 |
| rotavirus infection of the oropharynx and respiratory tract in young children. | oropharyngeal aspirates were obtained from 89 infants hospitalized with respiratory illnesses accompanied or not by diarrhea and 33 control patients without the diseases. rotavirus was detected from 25 of these patients by immunocytology, isolation of the virus in cultures of ma104 cells, or both. none of the control patients gave a positive result. the infection involves squamous cells and globlet cells probably originating from the oropharynx, and ciliated columnar epithelial cells from the re ... | 1991 | 1653306 |
| molecular analysis of the gene 6 from a porcine group c rotavirus that encodes the ns34 equivalent of group a rotaviruses. | the complete nucleotide sequence of the gene 6 from the porcine group (gp) c rotavirus strain cowden was determined from gene 6-specific clones selected from a cdna library and from viral transcript rna. the gene is 1348 nucleotides in length with a potential initiation codon beginning at nucleotide 25 and a stop codon at nucleotide 1231. the deduced protein contains 402 amino acids. comparison of the gene 6 from this gp c strain with sequences in the genbank data base indicated that this gene s ... | 1991 | 1653496 |
| identification of atypical rotaviruses in outbreaks of preweaning and postweaning diarrhea in québec swine herds. | intestinal contents of diarrheic pigs from 120 outbreaks of diarrhea were examined for the presence of atypical rotaviruses. pigs involved in these outbreaks were aged two days to five weeks and samples collected over a period of one year originated from different regions of the province of québec. samples were analyzed by polyacrylamide gel electrophoresis (page) for the presence of viral rna and genome profiles were compared to those of porcine rotavirus a/osu, b/ohio and c/cowden. based on el ... | 1991 | 1653639 |
| the shedding of group a rotavirus antigen in a newly established closed specific pathogen-free swine herd. | a longitudinal study was undertaken in a newly established specific pathogen-free (spf) swine herd to determine the dynamics of rotavirus antigen shedding in a closed swine facility. pregnant spf gilts which populated the herd, and their offspring, were monitored weekly for three consecutive lactations. fecal samples were assayed for the presence of group-specific viral antigen by a solid phase immunoassay (elisa). results indicate that in the week prior to farrow, 35% of samples from gilts/sows ... | 1991 | 1654666 |