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fungal growth inhibitory properties of new phytosphingolipid analogues.to study the growth inhibitory properties of a series of phytosphingosine (phs) and phytoceramide (phc) analogues.200818005346
beta-glucosidase of penicillium funiculosum. ii. properties and mycelial binding.the properties of two isozymes of beta-glucosidase of penicillium funiculosum (part i of this series) are described. the molecular weights of isozyme 1 was 2.3 x 10(5) by gel filtration and 1.2 x 10(5) by sds gel electrophoresis, indicating two subunits. the molecular weight of isozyme 2 was unusually low for a fungal beta-glucosidase: 1.6 x 10(4) by gel filtration and 3.7 x 10(4) in the presence of isopropanol. the two enzymes differed from other fungal beta-glucosidases in their substrate spec ...198518553737
hydrolysis of sugarcane bagasse by mycelial biomass of penicillium funiculosum. 198518553781
structural basis of poly(3-hydroxybutyrate) hydrolysis by phaz7 depolymerase from paucimonas lemoignei.the crystal structure of poly(3-hydroxybutyrate) (phb) depolymerase phaz7 purified from paucimonas lemoignei was determined at 1.90 a resolution. the structure consists of a single domain with an alpha/beta hydrolase fold in its core. the active site is analogous to that of serine esterases/lipases and is characterized by the presence of a catalytic triad comprising ser136, asp242, and his306. comparison with other structures in the protein data bank showed a high level of similarity with the ba ...200818706425
properties of selected hemicellulases of a multi-enzymatic system from penicillium funiculosum.a multi-enzymatic system from penicillium funiculosum displayed alpha-l-arabinofuranosidase, endo-1,4-beta-d-xylanase, beta-d-xylosidase and endo-1,3-1,4-beta-d-glucanase activities at high levels over a wide acidic ph range of 2.0 to 5.5. moreover, the ph stability was particularly extended over the wide range of ph of 2.0 to 8.0 with endo-1,3-1,4-beta-d-glucanase and endo-1,4-beta-d-xylanase; however, alpha-l-arabinofuranosidase and beta-d-xylosidase exhibited higher stability in the ph range ...200919502744
enzyme complex containing carbohydrases and phytase improves growth performance and bone mineralization of broilers fed reduced nutrient corn-soybean-based diets.one experiment was conducted to investigate the benefits of a multi-enzyme complex, containing carbohydrases (from penicillium funiculosum) and phytase (bacterial 6-phytase) activities, on the performance and bone mineralization of broiler chickens fed corn-soybean meal diets. a total of 2,268 male broilers were allocated to 9 treatments, replicated 6 times, in a randomized complete block design from 1 to 43 d. a positive control (pc) diet formulated to be adequate in nutrients and 4 reduced nut ...200919687277
cellulases from penicillium funiculosum: production, properties and application to cellulose hydrolysis.the objective of this work is to investigate the utilization of two abundant agricultural residues in brazil for the production and application of cellulolytic enzymes. different materials obtained after pretreatment of sugarcane bagasse, as well as pure synthetic substrates, were considered for cellulase production by penicillium funiculosum. the best results for fpase (354 u l(-1)) and beta-glucosidase (1,835 u l(-1)) production were observed when sugarcane bagasse partially delignified cellul ...201019902281
nitrogen source optimization for cellulase production by penicillium funiculosum, using a sequential experimental design methodology and the desirability function.the present study aimed at maximizing cellulase production by penicillium funiculosum using sequential experimental design methodology for optimizing the concentrations of nitrogen sources. three sequential experimental designs were performed. the first and the second series of experiments consisted of a 2(4) and a 2(3) factorial designs, respectively, and in the third one, a central composite rotational design was used for better visualizing the optimum conditions. the following nitrogen source ...201020013074
characterization of the family gh54 alpha-l-arabinofuranosidases in penicillium funiculosum, including a novel protein bearing a cellulose-binding domain.the soil deuteromycete penicillium funiculosum is characterized by its remarkable capacity to produce a wide variety of cellulolytic and hemicellulolytic enzymes. in the course of the genome sequencing of this industrial fungus, four different genes encoding glycosyl hydrolase family 54 (gh54)22 alpha-l-arabinofuranosidases were identified. three of them termed pfabfb1, pfabfb3, and pfabfb4 were highly similar, encoding proteins of 507, 508, and 505 amino acids, respectively. they exhibited stru ...201020333513
identification, characterization, and molecular cloning of a novel hyaluronidase, a member of glycosyl hydrolase family 16, from penicillium spp.hyaluronidase (haase) activity was detected in the culture supernatants of penicillium purpurogenum and penicillium funiculosum. the haase from penicillium spp. (haase-p) was a hyaluronate 4-glycanohydrolase, which catalyzed the endolytic hydrolysis of the β-1,4 glycosidic linkage, as do vertebrate haases. the gene encoding haase-p was cloned and expressed in escherichia coli. according to homology analyses of the deduced amino acid sequences, haase-p is not classified into any of the known haas ...201021094645
effect of competition and adverse culture conditions on aflatoxin production by aspergillus flavus through successive generations.strains of aspergillus flavus often degenerate with serial transfers on culture media, resulting in morphological changes and loss of aflatoxin production. however, degeneration does not readily occur in nature as indicated by the wild-type morphological characters of newly isolated strains and the high percentage of aflatoxigenic a. flavus from soil and crops in some geographic regions. in this study, three aflatoxin-producing strains of a. flavus were serially transferred using conidia for 20 ...200221156547
ameliorative symbiosis of endophyte (penicillium funiculosum lhl06) under salt stress elevated plant growth of glycine max l.experiments were conducted to investigate the role of a newly isolated endophytic fungus gmc-2a on physiology of host plant (glycine max. l cv. hwangkeum-kong) growing under salinity stress. gmc-2a was identified as a new strain of penicillium funiculosum on the basis of sequence homology and phylogenetic analysis of d1/d2 regions of 28s rdna. preliminary screening experiment showed that the culture filtrate (cf) of gmc-2a promoted the growth of waito-c, a dwarf gibberellin (ga) biosynthesis mut ...201121458283
gh10 xylanase d from penicillium funiculosum: biochemical studies and xylooligosaccharide production.the filamentous fungus penicillium funiculosum produces a range of glycoside hydrolases (gh). the xynd gene, encoding the sole p. funiculosum gh10 xylanase described so far, was cloned into the ppiczaa vector and expressed in methylotrophe yeast pichia pastoris, in order to compare the results obtained with the p. funiculosum gh11 xylanases data.201121466666
synthesis and evaluation of dioleoyl glyceric acids showing antitrypsin activity.previously, lešová et al. reported the isolation and identification of metabolite or-1, showing antitrypsin activity, produced during fermentation by penicillium funiculosum. the structure of or-1 was a mixture of glyceric acid (ga), esterified with c(14)-c(18) fatty acids, and oleic acid (c18:1) as the most predominant fatty acid (folia microbiol. 46, 21-23, 2001). in this study, dioleoyl d-ga and dioleoyl l-ga were synthesized via diesterification with oleoyl chloride, and their antitrypsin ac ...201121606621
Composition and antimicrobial activity of Seseli globiferum essential oil.The essential oil from aerial parts of Seseli globiferum Vis. obtained by hydrodistillation with Clevenger-type apparatus was analyzed by GC-MS. Twenty-eight compounds were identified, representing 99.4% of the total oil. The main components of the oil were sabinene (38.0%), alpha-pinene (21.2%) and beta-phellandrene (13.5%). The microbial growth inhibitory properties of the isolated essential oil were determined using the broth microdilution method against seven bacterial species: Salmonella ty ...201121922927
high performance sio(2)-nanoparticles-immobilized-penicillium funiculosum for bioaccumulation and solid phase extraction of lead.novel biosorbent systems were designed, investigated and implemented for bioaccumulation of pb(ii) from aqueous solutions. these are based on the combination of sio(2)-nanoparticles (n-si) with penicillium funiculosum fungus (pen) for the formation of (n-si-pen) as well as heat inactivated penicillium funiculosum (pen). the sio(2)-nanoparticles were also investigated as a solid sorbent phase. surface characterization and immobilization were examined and confirmed by using ft-ir and sem analysis. ...201122197335
hydrolysis of lignocelluloses by penicillium funiculosum cellulase. 198418553304
studies on cellulase production by a mutant-penicillium funiculosum uv-49.in search of hypercellulolytic microorganisms, ultraviolet irradiation carried out with penicillium funiculosum has yielded a superior mutant. the investigations reported in this article are shake flask studies on some important nutritional requirements of the mutant, namely, nitrogen source, carbon source, and inducers. the mutant shows an ability to metabolize inorganic nitrogen sources like urea and sodium nitrate both for growth and enzyme production. a comparison of the long-term saccharifi ...198418553529
molecular cloning, and characterization of a modular acetyl xylan esterase from the edible straw mushroom volvariella volvacea.a new volvariella volvacea gene encoding an acetyl xylan esterase (designated as vvaxe1) was cloned and expressed in pichia pastoris. the cdna contained an orf of 1047 bp encoding 349 amino acids with a calculated mass of 39 990 da. vvaxe1 is a modular enzyme consisting of an n-terminal signal peptide, a catalytic domain, and a cellulose-binding domain. the amino acid sequence of the enzyme exhibited a high degree of similarity to cinnamoyl esterase b from penicillium funiculosum, and acetyl xyl ...200717623028
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