Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| an antitumor cardenolide with inhibitory activity against pseudomonas pseudomallei. | the antibacterial activity of a new cardenolide, 7beta, 14beta - dihydroxy-5-card-20(22) enolide has been studied and it has been found to be active against pseudomonas pseudomallei. the new cardenolide also exhibited antitumor activity in a potato disc assay. | 1993 | 16414728 |
| virulence and susceptibility to phagocytosis of pseudomonas pseudomallei r- and s-forms for ground squirrels (citellus citellus l.). | virulence and susceptibility to phagocytosis of pseudomonas pseudomallei r- and s-forms for ground squirrels were studied. the alveolar macrophages and blood leucocytes (polymorphonuclear and mononuclear) was used. s-forms survived and multiplied intracellularly more actively in alveolar macrophages than r-forms and their virulence was considerably higher. | 1993 | 7506861 |
| burkholderia pseudomallei and melioidosis: be aware in temperate area. | 1993 | 7507550 | |
| pilot study of exposure to pseudomonas pseudomallei in northern vietnam. | 1993 | 7504340 | |
| the 1990-1991 outbreak of melioidosis in the northern territory of australia: epidemiology and environmental studies. | from november 1990 to june 1991 33 acute cases of melioidosis occurred in the northern territory, australia; 25 cases were reported in the capital city, darwin. we carried out an epidemiological investigation to exclude a common source outbreak, describe the risk factors for disease, and develop and institute appropriate control measures. we compared population based attack rates among various risk groups using logistic regression, and the demographic, medical and behavioral risk factors for mel ... | 1993 | 7512752 |
| application of indirect immunofluorescence microscopy to colony identification of pseudomonas pseudomallei. | indirect immunofluorescence microscopy was used as a colony identification method of pseudomonas pseudomallei isolates. the antisera against lipopolysaccharide and protein fractions of p. pseudomallei were prepared in guinea pigs and rabbits. with these antisera and fluorescence-labelled anti-guinea pig igg and anti-rabbit igg prepared in sheep (goat), indirect immunofluorescence microscopy was conducted on the colonies of p. pseudomallei and other species of bacteria. the overall results indica ... | 1993 | 7521644 |
| characterization of pseudomonas pseudomallei antigens by sds-polyacrylamide gel electrophoresis and western blot. | immunological characterization of various pseudomonas pseudomallei preparations was carried out by sds-page and western blot using sera from infected humans and from patients with other bacterial infections. somatic (som) and partially purified cell extracts (pce) gave more complex sds-page patterns: m(r) ranged from 86 to 12.7 and 48 to 10 kda, respectively. the culture-filtrated antigens (cf) from 3 different kinds of synthetic media consisted of fairly simple profiles with common bands m(r) o ... | 1993 | 7689760 |
| serology and carriage of pseudomonas pseudomallei: a prospective study in 1000 hospitalized children in northeast thailand. | throat swab cultures and indirect hemagglutination assay (iha) for pseudomonas pseudomallei were done in 1000 randomly selected children at a large hospital in northeast thailand. during 18 months, 17 children with melioidosis were admitted (0.46% of pediatric admissions excluding neonates born in the hospital). throat swab was positive for p. pseudomallei in 8 of these but in none of 1000 control children. iha seroprevalence rose at a conversion rate of 24% per year, from 12% in those 1-6 month ... | 1993 | 7678106 |
| identification of specific antigens of pseudomonas pseudomallei and evaluation of their efficacies for diagnosis of melioidosis. | current methods for the diagnosis of melioidosis are based on bacteriological culture. a number of serological tests currently available lack specificity and sensitivity. this is largely due to the use of crude antigens which results in a significant cross-reactivity with sera from individuals infected with other bacteria. in this study five different antigens were prepared and evaluated for their potential usefulness in diagnosis of melioidosis. these included a 19.5-kda antigen which was previ ... | 1993 | 7684750 |
| relationships among pseudomonas pseudomallei isolates from patients with recurrent melioidosis. | patients with melioidosis may present with recurrent infections after clinical resolution of their primary illness. because there has been no satisfactory typing scheme for pseudomonas pseudomallei, recrudescence could not be distinguished from reinfection. we determined the strain identity of primary and relapse isolates of p. pseudomallei from 25 patients with culture-proven melioidosis to answer whether secondary infections were due to the initial infecting strain or to the acquisition of a n ... | 1993 | 7686185 |
| survival of pseudomonas pseudomallei strains at 5 degrees c. | the ability of 15 pseudomonas pseudomallei strains of different origin to survive at 5 degrees c was tested, in comparison with the type strains of pseudomonas aeruginosa and pseudomonas cepacia. viable cells of each strain were suspended in tryptic soy broth (diluted 1:10) at a concentration of about 10(6) cfu/ml. the suspensions were kept at 5 degrees c, and the number of viable cells was determined by counting colonies every 10 days. four strains of p. pseudomallei, including the type strain ... | 1993 | 7686201 |
| interaction of insulin with pseudomonas pseudomallei. | pseudomonas pseudomallei is the causative agent of melioidosis, a disease being increasingly recognized as an important cause of morbidity and mortality in many regions of the world. an intriguing observation regarding melioidosis is that a significant percentage of patients who develop the disease have preexisting diabetes mellitus. in this regard, we have tested the hypothesis that insulin may modulate the growth of p. pseudomallei. we have demonstrated that insulin markedly inhibits the growt ... | 1993 | 7691742 |
| [spontaneous phage production in pseudomonas pseudomallei and in a range of hosts of melioidosis phages among representatives in the genus pseudomonas]. | a capacity to spontaneous phage production has been studied in 57 melioidosis museum strains. the strains were comparatively analyzed by the levels of frequencies of spontaneous phage production. it is established that 40 of 57 studied strains of pseudomonas pseudomallei are capable to spontaneously produce phages with frequency from 10(7) to 1. on the indicator lawn of p. mallei phages formed negative colonies of different morphology transparent, turbid and turbid with secondary growth in the c ... | 1993 | 7693232 |
| ability of pseudomonas pseudomallei malleobactin to acquire transferrin-bound, lactoferrin-bound, and cell-derived iron. | the ability of malleobactin to mobilize iron from transferrin and lactoferrin was examined in an equilibrium dialysis assay in the absence of bacteria. malleobactin was capable of removing iron from both transferrin and lactoferrin at ph values of 7.4, 6.0, and 5.0. however, the levels of iron mobilization were greater for transferrin than for lactoferrin at all the ph values used in the assay. the ability of pseudomonas pseudomallei to acquire iron from 30% iron-saturated transferrin and k562 h ... | 1993 | 7678587 |
| ribotype analysis of pseudomonas pseudomallei isolates. | no epidemiological typing system to differentiate among pseudomonas pseudomallei isolates has been available. ribotype analysis was developed and used to examine 74 clinical and 10 environmental isolates of p. pseudomallei from thailand. six p. pseudomallei ribotypes were identified from restriction fragment polymorphisms of ecori chromosomal digests. the predominant ribotype, a, was found in 59 of the isolates examined. by using patterns from hybridizations with sali, hindiii, and psti restrict ... | 1993 | 7679401 |
| molecular typing of pseudomonas pseudomallei: restriction fragment length polymorphisms of rrna genes. | the aim of this study was to develop a typing scheme for pseudomonas pseudomallei by comparison of patterns of restriction fragment length polymorphisms in rrna genes (ribotyping). bamhi restriction digests of 100 isolates from various animal (34), human (58), and environmental (6) sources, including six reference strains, were hybridized to escherichia coli 16s and 23s rrnas. a chemiluminescent labelling and detection system was used to visualize bands. on the basis of patterns, the strains wer ... | 1993 | 7681436 |
| latex agglutination test for identification of pseudomonas pseudomallei. | a latex agglutination test was developed and evaluated for the rapid presumptive identification of pseudomonas pseudomallei, the causative organism of melioidosis. the test was 100% sensitive for 52 isolates of ps pseudomallei and 100% specific when tested with other medically important pseudomonas species and enterobacteriaceae. a subsequent field trial, with clinical specimens from patients with suspected melioidosis, confirmed the sensitivity and specificity of the test. | 1993 | 7684405 |
| musculoskeletal melioidosis. | during a four-year period, twenty-one patients were found to have melioidosis of the musculoskeletal system caused by pseudomonas pseudomallei. the melioidotic patients were matched with thirty-nine patients who had a musculoskeletal infection due to some other organism, and the two groups were compared with regard to the presence of concurrent disease. the indirect hemagglutination test for melioidosis was positive for all of the melioidotic patients; in thirteen, cultures also were positive. f ... | 1993 | 8258552 |
| resistance of pseudomonas pseudomallei growing as a biofilm on silastic discs to ceftazidime and co-trimoxazole. | we have examined the resistance of pseudomonas pseudomallei biofilm cells to ceftazidime and cotrimoxazole. a large number of these biofilm cells remained viable at 12 and at 24 h, except in the biofilm treated with 200 times the mic of ceftazidime. the inherent resistance of p. pseudomallei biofilms to conventional antibiotics may explain the lack of success in the treatment of the chronic manifestations of this bacterial infection. | 1993 | 7694545 |
| [transduction of pseudomonas mallei bacteria]. | the transducing ability of 17 pseudomonas pseudomallei bacteriophages has been studied. five of them were found to be capable of transducing the transposon tn7 markers tpr, smr into the strain pseudomonas mallei c-5. the transduced bacterial recipients became lysogenic and acquired resistance to trimethoprim and streptomycin. transduction of transposon tn7 has been confirmed by dna-dna hybridization. | 1993 | 8396725 |
| epidemiology of sudden unexpected death syndrome among thai migrant workers in singapore. | a total of 235 cases of sudden unexpected death syndrome (suds) among apparently healthy male thai migrant workers in singapore were reported between 1982 and 1990. most of the deaths occurred during sleep and 13% were not sleep-related. the median age at the time of death was 33 years and the median interval between arrival and death was 8 months. these deaths occurred singly and sporadically throughout the year. post-mortem examination revealed few abnormal findings except for haemorrhagic con ... | 1993 | 8449652 |
| the 1990-1991 outbreak of melioidosis in the northern territory of australia: clinical aspects. | from november 1990 to june 1991, 33 cases of acute melioidosis were diagnosed in tropical northern territory, australia during an exceptionally wet monsoon. eighteen (55%) were alcoholic, 16 (48%) diabetic and only 4 (12%, all survivors) had no risk factors. twenty-seven (82%) were considered recent infection, with an incubation period of 3-21 days (mean 14) documented in eight cases with presumed cutaneous inoculation. fourteen patients presented with pneumonia (4 septicemic) and of 11 others w ... | 1993 | 8160049 |
| chronic melioidosis: a report of the first case in japan. | a 41-year-old japanese male with uncontrolled diabetes mellitus and alcoholic liver dysfunction developed melioidosis after his business trip to indonesia and singapore in 1988. his disease started with spiked fever on the following day after extraction of a tooth, and a liver abscess developed, followed by abscesses in the spleen and in the subphrenic space. in spite of splenectomy and intensive antimicrobial treatments for three months, he developed parotitis, prostatitis, and abscess of the r ... | 1993 | 8468500 |
| separation of antigenic glycoprotein fractions from cell-free homogenate of pseudomonas pseudomallei and characterization as tyrosine phosphatase. | cell-free extracts were prepared from pseudomonas pseudomallei cells by freezing-thawing, sonication, and differential ultracentrifugation. the extracts were subjected to column chromatography with deae-sepharose to obtain glycoprotein fractions. the fractions showed acid phosphatase activity to p-nitrophenyl phosphate, tyrosine phosphate, serine phosphate, but not to threonine phosphate. they were highly antigenic when tested by immunofluorescence assay with the sera of melioidosis patients. | 1994 | 7539939 |
| immunofluorescence microscopy for the rapid diagnosis of melioidosis. | a direct immunofluorescent antibody test (dif) was developed for the rapid diagnosis of melioidosis, a potentially fatal infection caused by pseudomonas pseudomallei. in a clinical evaluation of 369 sputum, pus, or urine specimens from 272 patients with suspected melioidosis, the dif had a sensitivity of 73% and a specificity of 99% compared with culture. using this dif, a confident diagnosis of melioidosis can now be made within two hours of admission to hospital, compared with the delay of two ... | 1994 | 8027383 |
| [specific immune complexes and neutrophil injury test in the diagnosis of experimental glanders and melioidosis]. | the authors have demonstrated the possibility of using solid-phase enzyme immunoassay to detect specific circulating immune complexes without their preliminary separation in guinea pigs infected with pseudomonas mallei and pseudomonas pseudomallei. they have examined the prospects of using neutrophil injury test and solid-phase enzyme immunoassay for the detection of specific immune complexes as additional tests used to predict the outcome of an infectious process in glanders and melioidosis. | 1994 | 7894914 |
| effects of tunicamycin on the ph-activity pattern of acid phosphatase in pseudomonas pseudomallei. | the liquid culture of pseudomonas pseudomallei shows a complex feature in in the ph-activity pattern of acid phosphatase, not a single peak curve. there was an evident tendency that the higher activity shifted to the higher ph range with the growth of culture. the culture in the presence of tunicamycin (20 micrograms/ml) showed a decreased activity selectively in the higher ph range, while the activity in the lower ph was more heat-labile. the bacterial cells grown on agar plates containing tuni ... | 1994 | 7529943 |
| ultrastructural characteristics of some bacteria after treatment with lubrol w. | specific ultrastructural changes occurred mainly in the cell wall and cytoplasmic membrane of listeria monocytogenes, salmonella typhimurium, pseudomonas pseudomallei and pseudomonas aeruginosa bacteria when treated with 0.5% and 1% lubrol w1 by means of transmission and scanning electron microscopy. | 1994 | 7700161 |
| the epidemiology of melioidosis in ubon ratchatani, northeast thailand. | melioidosis, or infection with pseudomonas pseudomallei is an important cause of morbidity and mortality in south east asia and northern australia. the epidemiology of melioidosis in ubon ratchatani, northeast thailand was studied over a 5-year period from 1987 to 1991. | 1994 | 7860160 |
| [pulmonary melioidosis. report of 4 cases and literature reviewed]. | in this report, we present 4 patients with pulmonary melioidosis. the literatures on epidemiology, clinical manifestation, roentogenology, diagnosis and therapeutics of melioidosis are reviewed. | 1994 | 7530604 |
| susceptibility of pseudomonas pseudomallei to some newer beta-lactam antibiotics and antibiotic combinations using time-kill studies. | 1994 | 7512547 | |
| molecular typing of pseudomonas pseudomallei from imported primates in britain. | 1994 | 7526531 | |
| detection of pseudomonas pseudomallei antigen in urine for the diagnosis of melioidosis. | an enzyme-linked immunosorbent assay using a fluorescein isothiocyanate (fitc)-anti-fitc amplification system, has been developed to detect pseudomonas pseudomallei antigen in urine. the assay was evaluated in 135 patients with acute melioidosis, 194 hospitalized patients with other disorders, and 40 healthy controls. antigen was detected in the urine of 123 (91%) patients with melioidosis. urinary antigen was found in 85 (96%) of 89 patients with septicemic melioidosis, all six patients with p. ... | 1994 | 7527187 |
| [identification of the bacterium pseudomonas mallei using pseudomonas pseudomallei bacteriophages]. | phage production by pseudomonas pseudomallei and pseudomonas mallei strains has been studied. 32 p. pseudomallei bacteriophages have been isolated. their spectrum of lytic action against p. pseudomallei, p. mallei and other pseudomonas sp. has been defined. it has been shown that p. pseudomallei bacteriophages pp19, pp23, pp33 may be used for identification p. mallei among related pseudomonas. | 1994 | 7527479 |
| melioidosis survey in kenya. | 1994 | 7518617 | |
| detection of pseudomonas pseudomallei by pcr and hybridization. | a molecular method for the detection of pseudomonas pseudomallei was developed on the basis of the differences in the 23s rrna sequences of related species of the genus pseudomonas. an 18-base oligonucleotide probe, designed following partial sequencing of 23s ribosomal dna (rdna), was used for the identification and detection of p. pseudomallei either by hybridization or by direct pcr. optimal detection was obtained by hybridization of the probe with pcr-amplified rdna rather than with total ge ... | 1994 | 7519629 |
| melioidosis in a diabetic sailor. | melioidosis was diagnosed in a diabetic sailor who presented with a history and chest radiograph that suggested tuberculosis. melioidosis is a tropical disease with protean manifestations: from asymptomatic infection to chronic cavitary lung disease to overwhelming sepsis. the diagnosis is easily made, even in nonendemic areas when duly considered by the clinicians and microbiology laboratory. ceftazidime has dramatically improved outcomes in hospitalized patients with severe melioidosis. | 1994 | 7521816 |
| pseudomonas pseudomallei isolates collected over 25 years from a non-tropical endemic focus show clonality on the basis of ribotyping. | between 1966 and 1991, melioidosis, a disease caused by pseudomonas pseudomallei that is mostly confined to tropical regions, occurred in farm animals and a farmer in temperate south-west western australia. using an escherichia coli probe containing a ribosomal rna operon, p. pseudomallei dna from isolates from 8 animals, a soil sample and the human case showed an identical ribotype on southern blotting. the ribotype was different from the 3 commonest ribotypes seen in tropical australia. this m ... | 1994 | 7523158 |
| interaction of pseudomonas pseudomallei with macrophages. | 1994 | 7525379 | |
| fatal melioidosis in a tourist returning from thailand. | severe infections with pseudomonas pseudomallei, the causative agent of melioidosis, is a common cause of community acquired septicaemia in south east asia and parts of northern australia, but infection in travellers returning to the united kingdom is extremely rare. we describe the case of a tourist who acquired melioidosis in thailand. despite intensive intravenous therapy with antibiotics to which the organism was sensitive, the patient's infection proved fatal. ps. pseudomallei was isolated ... | 1994 | 7525733 |
| recent advances in biomedical sciences of burkholderia pseudomallei (basonym: pseudomonas pseudomallei). | 1994 | 7526019 | |
| effect of sorbitan monolaurate polyoxyalkylene (tween 20) on the ultrastructure of some bacteria. | the ultrastructure of listeria monocytogenes, salmonella typhimurium, pseudomonas pseudomallei and pseudomonas aeruginosa treated with 0.5 and 1% tween 20 was studied by means of transmission and scanning electron microscopy. specific changes in the cell wall and cytoplasmic membrane were observed. | 1994 | 7539732 |
| neutralizing monoclonal antibodies to an extracellular pseudomonas cepacia protease. | pseudomonas cepacia produces at least two extracellular proteases with apparent molecular masses of 36,000 and 40,000 da. the 36-kda protease has high proteolytic activity and the 40-kda protease has low proteolytic activity with hide powder azure as a substrate. monoclonal antibodies (mabs) were raised against the purified 36- and 40-kda proteases. several mabs directed against the 36-kda protease were found to recognize the 40-kda protease by western immunoblot analysis. similarly, a mab direc ... | 1994 | 7516312 |
| isolation and characterization of the outer-membrane proteins of burkholderia (pseudomonas) pseudomallei. | membranes obtained from whole-cell lysates of burkholderia (pseudomonas) pseudomallei (strain 319a) were separated into four fractions by sucrose density gradient centrifugation. membranes were characterized by enzymic and chemical analyses, and by sds-page. cytoplasmic membranes and two forms of outer membranes (om-1, om-2) were detected. the major outer-membrane proteins had m(r) values of 70,000, 38,000, 31,000, 24,000 and 17,000. to determine which outer-membrane proteins were common to b. p ... | 1994 | 7516793 |
| purification and characterization of a protease from pseudomonas pseudomallei. | pseudomonas pseudomallei is the causative agent of melioidosis, a glanders-like disease of humans and animals. the pathogenesis of melioidosis is not well understood, and the role of various extracellular enzymes produced by p. pseudomallei in the development of this disease is not known. the present studies were designed to purify and characterize an extracellular protease produced by p. pseudomallei isolates and to test the hypothesis that this protease may play a role in melioidosis. the prot ... | 1994 | 7528633 |
| [epidemiology of melioidosis in china]. | from 1975 to 1989, a total of 73 strains of p. pseudomallei was isolated from the water samples and the pathological samples of human and domestic animals in 13 counties and cities located different latitude from four provinces qiong, yue, gui and xiang in china. serological investigation demonstrated that the geographical distribution of the organism had a significant correlation with the positive rate of antibodies against p. pseudomallei and the native foci of the organisms distributed over t ... | 1994 | 7532109 |
| isolation and characterization of pseudomonas pseudomallei flagellin proteins. | flagellin proteins from several different strains of pseudomonas pseudomallei have been isolated and purified to homogeneity by mechanical shearing and differential centrifugation techniques. analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis yielded flagellin monomer protein bands with an estimated m(r) of 43,400. no lipopolysaccharide contamination of the purified protein preparations was detectable by silver staining of flagellin displayed on polyacrylamide gels and by west ... | 1994 | 7513308 |
| [the immunodiagnosis of melioidosis by a solid-phase variant of radioimmunological analysis (ria)]. | a variant of the solid-phase radioimmunoassay (ria) has been developed for the detection of specific immunoglobulins in laboratory animals infected with pseudomonas pseudomallei. the proposed method has advantages over the indirect hemagglutination test and the enzyme immunoassay in its sensitivity and specificity. the newly developed ria variant, based on group-specific antigens 6 + d, makes it possible to classify the strain causing the disease with the asian or australian serovar of p. pseudo ... | 1994 | 7524250 |
| melioidosis. another etiology of granulomatous osteomyelitis. report of 2 cases. | two cases of granulomatous osteomyelitis caused by melioidosis are reported. histologically, the granulomatous lesions were indistinguishable from tuberculosis and required confirmation with microbiological culture. it is important that melioidosis be considered in the differential diagnosis of granulomatous osteomyelitis since the treatment is quite different from tuberculosis. | 1994 | 7525133 |
| outbreak of plague-like illness caused by pseudomonas pseudomallei in maharashtra, india. | 1994 | 7527114 | |
| construction of a specific dna probe for diagnosis of melioidosis and use as an epidemiological marker of pseudomonas pseudomallei. | pseudomonas pseudomallei is a causative agent of melioidosis. the disease manifestations range from fulminant sepsis to asymptomatic seroconversion. in septicemic cases, a mortality rate of 80-90% is reported. rapid and specific diagnosis has become important to the clinical microbiology laboratory. we have developed a p. pseudomallei-specific dna probe. the cloned fragment, herein designated pkku-s23l, contained 1.5 kb of p. pseudomallei chromosomal dna. a radioactively labelled pkku-s23l inser ... | 1994 | 7518036 |
| passive protection of diabetic rats with antisera specific for the polysaccharide portion of the lipopolysaccharide isolated from pseudomonas pseudomallei. | polyclonal and monoclonal antisera raised to tetanus toxoid-conjugated polysaccharide of lipopolysaccharide (lps) and purified lps of pseudomonas pseudomallei that reacted with a collection of 41 strains of this bacterium from 23 patients are described. the common antigen recognized by these sera was within the polysaccharide component of the lps of the cells. the sera were specific for p pseudomallei in that none of 37 strains of other bacteria, including 20 gram-negative and three gram-positiv ... | 1994 | 22346496 |
| imaging patterns in melioidosis. | melioidosis is an infectious disease caused by pseudomonas pseudomallei. it is seldom diagnosed promptly and, if untreated, can lead to an 80-100% mortality rate. twenty-eight patients with melioidosis were identified over a 6 year period, and their imaging patterns were analysed. respiratory infections were the commonest form of presentation, frequently shown as diffuse airspace consolidation, and accounted for the highest mortality. visceral and musculoskeletal infections were associated with ... | 1995 | 7487762 |
| selective broths for the isolation of pseudomonas pseudomallei from clinical samples. | 1995 | 7538232 | |
| plague in india. | 1995 | 7529862 | |
| [transport medium for pathogenic pseudomonads]. | a transporting medium was designed for the transportation and preservation for 5 to 7 days of material infected with agents of malleus and melioidosis. foreign contaminating microorganisms gradually die in this medium, whereas solitary cells of malleus and melioidosis agents (100 bacterial cells per 0.1 ml) survive. | 1995 | 7538858 |
| [differentiation between glanders and melioidosis]. | 1995 | 7538860 | |
| [laboratory control of effectiveness of chemotherapy in melioidosis]. | an undoubtable role of the melioidosis germ persistence in the form of l-variants with the development of dth in the relapses of the disease was stated. serological reactions such as cft and phar were shown to be of the diagnostic value but it was by far lower than the allergotest. no correlation between the antibody titers in the tested sera and the isolation of the bacterial or l-variants of the melioidosis germ was observed. the criteria of the recovery from melioidosis were: stable normaliza ... | 1995 | 7541620 |
| purification and characterization of an exopolysaccharide of burkholderia (pseudomonas) pseudomallei. | burkholderia pseudomallei (basonym pseudomonas pseudomallei) is the causative organism of melioidosis, a disease which is recognized as a major public health problem primarily in southeast asia and northern australia. in this paper, we report on the identification, purification, and characterization of a species-specific exopolysaccharide of b. pseudomallei. after immunization of mice with a b. pseudomallei strain exhibiting mucoid growth characteristics, we isolated an immunoglobulin g1 monoclo ... | 1995 | 7558305 |
| monoclonal antibodies to pseudomonas pseudomallei and their potential for diagnosis of melioidosis. | monoclonal antibodies (mabs) specific for pseudomonas pseudomallei antigens were produced by immunizing balb/c mice with a crude whole cell extract. hybrids secreting mabs specific for p. pseudomallei antigens were identified by an indirect enzyme-linked immunosorbent assay (elisa) against a panel of crude whole cell extracts from p. pseudomallei, p. cepacia, p. aeruginosa, p. putida, p. alcaligenes, xanthomonas maltophilia, klebsiella pneumoniae, escherichia coli, enterobacter cloacae, salmonel ... | 1995 | 7535014 |
| latex agglutination for rapid detection of pseudomonas pseudomallei antigen in urine of patients with melioidosis. | a latex agglutination test for the detection of pseudomonas pseudomallei antigen in urine was evaluated for the rapid diagnosis of melioidosis. with unconcentrated urine, antigen was detected in only 18% of patients with melioidosis overall. however, when urine was concentrated 100-fold, antigen was detected in 47% overall and in 67% of patients with septicaemia or disseminated infection, in whom a rapid diagnosis is most important. the specificity of the test was 100%. these results compared fa ... | 1995 | 7538150 |
| isolation of pseudomonas pseudomallei from soil in north-eastern thailand. | in order to optimize the recovery from soil of pseudomonas pseudomallei, the cause of melioidosis, 3 selective broths were compared. a basal salt solution containing l-threonine (tbss) performed significantly better than trypticase soy broth containing crystal violet and colistin 50 mg/l (cvc50), both in isolation rate and suppression of overgrowth of other organisms, but the addition of colistin to tbss gave the best results overall. in a survey in north-eastern thailand, p. pseudomallei was re ... | 1995 | 7538233 |
| cellular lipid and fatty acid compositions of burkholderia pseudomallei strains isolated from human and environment in viet nam. | viet nam is known as an endemic area of melioidosis but its etiologic agent originated in viet nam was not extensively studied. for the first time, we analyzed the cellular lipid and fatty acid compositions of 15 vietnamese isolates of burkholderia pseudomallei, 10 from humans and 5 from the environment. cellular lipid compositions were analyzed by two-dimensional thin-layer chromatography on silica gel g plates. cellular fatty acid methyl esters were analyzed by gas chromatography (gc) and gas ... | 1995 | 7540251 |
| pseudomonas pseudomallei-insulin interaction. | 1995 | 7543888 | |
| comparison of pseudomonas pseudomallei from humans, animals, soil and water by restriction endonuclease analysis. | pseudomonas pseudomallei isolates from 62 human, 17 animal, 3 soil and 3 water samples were examined by genomic dna digestion with psti. five major (re i, ii, iii, iv, v) reproducible restriction patterns were observed, with most (56/62) of the human isolates displaying re i (30/62), ii (5/62), iii (15/62), iv (4/62), v (2/62), and the animal (16/17), soil (2/3), water (3/3) isolates showing predominantly re ii profiles. six human and one soil isolates showed patterns different from those of re ... | 1995 | 7570138 |
| clinically practical seminested pcr for burkholderia pseudomallei quantitated by enzyme immunoassay with and without solution hybridization. | diagnosis of melioidosis, an infectious disease caused by burkholderia pseudomallei (formerly pseudomonas pseudomallei), is made initially by antibody testing, which is not always sensitive or specific. we have developed two seminested pcr protocols combined with enzyme immunoassay (eia) to detect the conserved ribosomal regulatory region of b. pseudomallei. both pcrs used one biotinylated primer for capturing pcr products on eia plates. one system, termed solution hybridization eia (sheia), hyb ... | 1995 | 7559961 |
| visceral abscess in melioidosis. | a retrospective analysis was done in 81 patients with visceral abscess due to melioidosis treated at khon kaen hospital, northeastern thailand from 1985 to 1993. the clinical presentations were fever 100 per cent, abdominal pain 39 per cent, cough 34.8 per cent, abdominal tenderness 27.5 per cent and palpable mass 24.6 per cent. the laboratory findings were not diagnostic of the etiology. the abscesses were detected by ultrasonography in 97.25 per cent and computed tomography 2.25 per cent. the ... | 1995 | 7561544 |
| burkholderia pseudomallei and indian plague-like illness. | 1995 | 7564697 | |
| cloning and sequencing of the genes involved in glyphosate utilization by pseudomonas pseudomallei. | thirty-four strains of pseudomonas pseudomallei isolated from soil were selected for their ability to degrade the phosphonate herbicide glyphosate. all strains tested were able to grow on glyphosate as the only phosphorus source without the addition of aromatic amino acids. one of these strains, p. pseudomallei 22, showed 50% glyphosate degradation in 40 h in glyphosate medium. from a genomic library of this strain constructed in puc19, we have isolated a plasmid carrying a 3.0-kb dna fragment w ... | 1995 | 7574593 |
| oligonucleotide primers designed to differentiate pathogenic pseudomonads on the basis of the sequencing of genes coding for 16s-23s rrna internal transcribed spacers. | universal primers targeting conserved sequences flanking the 3' end of the 16s and the 5' end of the 23s rrna genes (rdnas) were used to amplify the 16s-23s rdna internal transcribed spacers (its) from eight species of pseudomonads which have been associated with human infections. amplicons from reference strains of pseudomonas aeruginosa, pseudomonas cepacia, pseudomonas gladioli, pseudomonas mallei, pseudomonas mendocina, pseudomonas pickettii, pseudomonas pseudomallei, and xanthomonas maltoph ... | 1995 | 7583922 |
| [pseudomonas pseudomallei and pseudomonas mallei--capsule-forming bacteria]. | the specific features of the submicroscopic structure of the causative agents of melioidosis and glanders, grown in culture media and at the initial stages of experimental infection in guinea pigs and golden hamsters, were studied with the use of electronic cytochemistry and immunocytochemistry. p. pseudomallei and p. mallei were found to be capsule-forming bacteria. according to the data obtained with the use of electronic cytochemistry, the basis of the capsular substance p. pseudomallei and p ... | 1995 | 8525729 |
| melioidosis in a goat. | melioidosis was diagnosed in an adult boer goat ewe. the owner initially noticed swelling of one half of the udder. several firm nodules developed in the affected part of the udder 2 weeks later. at necropsy, abscesses containing a creamy to yellowish-green exudate were found in the mammary gland and one of the kidneys. pure cultures of pseudomonas pseudomallei were isolated from the abscesses in the udder and inoculation of male guinea-pigs with the causative bacterium, produced a shwartzman re ... | 1995 | 8544164 |
| electron microscopy study of the mode of growth of pseudomonas pseudomallei in vitro and in vivo. | the mode of growth of pseudomonas pseudomallei in culture media and in the lung tissue of infected humans and animals was studied using transmission electron microscopy (tem) and scanning electron microscopy (sem). in culture media, p. pseudomallei cells were seen to be entrapped in microcolonies within large amounts of intercellular fibrous material. the lung tissue of infected humans and animals showed that bacterial cells growing in lung tissue were surrounded by radially arranged fibres that ... | 1995 | 8544219 |
| [pseudomonas mallei and pseudomonas pseudomallei: introduction and maintenance of natural and recombinant plasmid replicons]. | comparative analysis of recipient activity of pseudomonas mallei, pseudomonas pseudomallei, and pseudomonas cepacia strains towards naturally occurring and recombinant plasmid replicons was carried out. autonomic broad host range vector plasmids based on rsf1010(incq) and psa(incw) replicons as well as integrative vectors based on psup202(co1e1) replicon have been constructed. the study has shown that naturally occurring plasmids rsf1010(incq), psa(incw), r15(incn), and rp4(incp) are being effic ... | 1995 | 7541512 |
| rapd analysis of isolates of burkholderia pseudomallei from patients with recurrent melioidosis. | twenty-seven isolates of burholderia pseudomallei (formerly pseudomonas pseudomallei) from ten patients with recurrent melioidosis were analysed by rapd. in two cases rapd patterns in recurrent isolates differed from the original isolates; one was considered a likely reinfection while the other may represent relapse from one of two strains initially infecting the patient. in two cases where a change in antibiotic resistance had occurred between original and relapse isolates, slight changes in ra ... | 1995 | 7543859 |
| [the isolation and characteristics of cloned strains of pseudomonas pseudomallei phages]. | on the basis of museum strains of the melioidosis agent pseudomonas pseudomallei, 14 pure lines of bacteriophages were isolated belonging to morphological groups iv and v according to the classification by a. s. tikhonenko. these phages show a broad bacteriolytic range as far as museum p. pseudomallei cultures are concerned. it was determined that some phages isolated from the same melioidosis culture and belonging to the same morphological type display different bacteriolytic activity. it is de ... | 1995 | 7544671 |
| structural characterization of the lipopolysaccharide o antigens of burkholderia pseudomallei. | a serologically typical strain of burkholderia pseudomallei (strain 304b) was found to produce two s-type lipopolysaccharides (lps) differing in the chemical structures of their o-polysaccharide (o-ps) components. structural analysis revealed that one o-antigenic polysaccharide (o-ps i) is an unbranched high-molecular-weight polymer of 1,3-linked 2-o-acetyl-6-deoxy-beta-d-manno-heptopyranose residues. the other lps o antigen (o-ps ii) is an unbranched polymer of repeating disaccharide units havi ... | 1995 | 7543882 |
| subdivision of burkholderia pseudomallei ribotypes into multiple types by random amplified polymorphic dna analysis provides new insights into epidemiology. | ribotyping has previously been used for epidemiological studies of burkholderia pseudomallei (previously pseudomonas pseudomallei). we show here that random amplified polymorphic dna (rapd) analysis allows subdivision of strains of the same ribotype. with five different primers, no two epidemiologically unrelated isolates of any single ribotype in this study of 102 isolates from humans, goats, cats, and soil had identical rapd patterns. conversely, rapd analysis showed clonality for isolates fro ... | 1995 | 7545176 |
| in vitro activity of tosufloxacin against pseudomonas pseudomallei, salmonella spp. and other enterobacteriaceae. | 1995 | 8549324 | |
| elevated serum procalcitonin levels in patients with melioidosis. | the prognostic value of serum procalcitonin levels in 43 patients with acute melioidosis, an infection with a wide range of clinical manifestations, was assessed. in eight patients with mild localized infections, the median procalcitonin levels were 0.13 ng/ml (range, 0.02-0.46 ng/ml), which were similar to those in 19 healthy controls (median, 0.07 ng/ml; range, 0.03-0.15 ng/ml). in the patients with severe infections, the initial procalcitonin levels were significantly higher in the patients w ... | 1995 | 7756489 |
| musculoskeletal melioidosis: clinical and imaging features. | melioidosis is a tropical infection caused by a gram-negative bacillus, pseudomonas pseudomallei. the disease manifests initially as localized suppurative lesions and can progress to acute disseminated septicemia with 65-90% mortality if inadequately treated. musculoskeletal involvement is common. the purpose of this study was to describe the clinical features and imaging appearances of musculoskeletal melioidosis. | 1995 | 8545645 |
| the histopathology of human melioidosis. | we examined human tissues infected by burkholderia (pseudomonas) pseudomallei which is endemic in malaysia to study the types of inflammation invoked, and to look for histopathological clues to its diagnosis. the lesions which varied from acute to chronic granulomatous inflammation were not tissue-specific. in five autopsy cases, the inflammation was usually a focal or diffuse, acute necrotising inflammation with varying numbers of neutrophils, macrophages, lymphocytes and 'giant cells'. the 'gi ... | 1995 | 7713483 |
| localised melioidotic osteomyelitis. | melioidosis is an uncommon infection caused by a gram-negative bacillus, pseudomonas pseudomallei. only a few case reports of orthopaedic infection have been published in english, and most were of isolated septic arthritis or secondary to melioidosis of another organ. we have reviewed ten patients with localised melioidotic osteomyelitis; six had underlying conditions. we discuss the importance of obtaining a bacteriological diagnosis, and of surgical debridement as well as appropriate antibioti ... | 1995 | 7744934 |
| chronic osteomyelitis due to burkholderia pseudomallei. | 1995 | 7752966 | |
| prognostic significance of quantitative bacteremia in septicemic melioidosis. | pour-plate blood cultures were performed for 418 adult patients with suspected septicemic melioidosis in order to determine the relationship between quantitative bacterial counts in blood and mortality. of 108 patients whose hemocultures yielded burkholderia pseudomallei, 53% had < 10 cfu/ml and 24% had > 100 cfu/ml. high blood bacterial counts were more common than reported previously with regard to other gram-negative septicemias and were significantly associated with the development of hypote ... | 1995 | 8749644 |
| burkholderia pseudomallei and indian plague-like illness. | 1995 | 7564767 | |
| pulmonary melioidosis. | melioidosis is the name given to all diseases caused by the bacterium pseudomonas pseudomallei. melioidosis is a tropical disease and prevails in parts of southeast asia, northern australia, and central and south america. however, in recent years, cases of melioidosis have been reported in the united states and other areas. the organism can infect any organ system, although the lung is the most common organ affected. pulmonary melioidosis presents either as an acute fulminant pneumonia or as an ... | 1995 | 7587451 |
| [monoclonal antibodies in the differentiation of pseudomonas mallei and pseudomonas pseudomallei]. | 1995 | 8553724 | |
| [the acid exopolysaccharide of the causative agent of melioidosis]. | 1995 | 8553726 | |
| [the production of hybridomas producing monoclonal antibodies to the causative agent of melioidosis by using antigen-stimulated lymphocytes in an in-vitro system]. | 1995 | 8553782 | |
| serodiagnosis of melioidosis in malaysia. | current diagnosis of melioidosis is based on bacterial culture and/or serology which is becoming increasingly useful. an igm-elisa using heat-killed whole cells of pseudomonas pseudomallei was developed and compared with an indirect haemagglutination technique (ihat) and an indirect immunofluorescent technique(ifat). the igm-elisa using a p:n ratio of > or = 2 had a sensitivity of 91% and a specificity of 96%. all 3 assays were further used in a seroepidemiological survey amongst different group ... | 1995 | 8553097 |
| postantibiotic effects and burkholderia (pseudomonas) pseudomallei: evaluation of current treatment. | the postantibiotic effects (paes) of 16 antibiotics or antibiotic combinations currently used against burkholderia (pseudomonas) pseudomallei were determined. the beta-lactams had zero paes, while the carbapenems and ciprofloxacin induced median paes of 1 and 5 h, respectively. these results emphasize the need to maintain antibiotic levels above inhibitory concentrations in blood throughout the dosing interval in cases of severe melioidosis. | 1995 | 8619598 |
| use of culture-filtrated antigen in an elisa and a dot immunoassay for the diagnosis of melioidosis. | an enzyme-linked immunosorbent assay (elisa) and a dot immunoassay with culture-filtrated antigen were developed for detection of burkholderia pseudomallei specific antibodies in melioidosis patients. sixty-eight sera of bacteriologically confirmed melioidosis patients, 45 sera of other bacterial infected patients and 80 sera of healthy blood donors from endemic area were investigated. the samples were subjected to those assays im comparison with indirect hemagglutination (iha). the sensitivity, ... | 1995 | 8629069 |
| quantitative recovery of burkholderia pseudomallei from soil in thailand. | melioidosis is common in north-eastern thailand, but is reported rarely from the adjacent areas of central thailand, although rice farming is common to both regions. quantitative soil cultures for burkholderia pseudomallei were therefore prepared on 12 rice farms in both regions. b. pseudomallei was isolated from a similar proportion of rice fields in the central region (6/12) and in the north-east (7/12). within the culture-positive sites, the number of b. pseudomallei colony-forming units (cfu ... | 1995 | 8560518 |
| survival of burkholderia pseudomallei in the absence of nutrients. | 1995 | 8560519 | |
| [a methodology for determining reservoirs of the causative agents of sapronoses]. | comparative ecological strategy for choosing the causative agents of melioidosis and glanders, pseudotuberculosis and plague after mcarthur [correction of macartura] and wilson were studied. the kinetic analysis of the populations of these bacilli in the steppe neocultured chestnut soil formed a basis of the study with the consideration of data on their growth and death in artificial culture media. to identify the types of selection, the authors defined the rate of growth, the level of populatio ... | 1995 | 8587519 |
| [the outlook for improving the immunoglobulin preparations for the detection and identification of the causative agents of glanders and melioidosis]. | the paper summarizes the data concerning the production and study of monoclonal antibodies (mab) to the diagnostically significant glanders and melioidosis bacillus antigens. it evaluates the efficiency of using mab in the gel immunodiffusion and agglutination tests as a basis of new-generation preparations for fluorescent antibody assay, indirect hemagglutination test which are used while detecting and identifying pathogenic pseudomonads. the paper defines the quality indices for monoclonal lum ... | 1995 | 8587520 |
| in vitro activity of sparfloxacin against selected enterobacteriaceae and pseudomonaceae including ps. pseudomallei (melioidosis). | 1995 | 8904106 | |
| geographical distribution of pseudomonas pseudomallei in china. | 1,366 samples of soil and water from southern china coastal provinces were examined for pseudomonas pseudomallei. data showed that 58 samples were positive for this bacteria, which is primarily distributed in hainan province and the coastal region of mainland. this paper confirmed the environmental presence of p. pseudomallei in china and showed that the distribution of this pathogen has at least reached a latitude of 25.5 degrees north. | 1995 | 9139366 |
| melioidosis presenting as epididymo-orchitis. | we report a rare case of suppurative epididymo-orchitis caused by pseudomonas pseudomallei in a 56-year-old male. this is a gram negative bacillus found mainly in tropical zones. diagnosis was reached by culture of the organisms after drainage of the scrotal abscess, and the patient was treated by a course of oral chloramphenicol 500 mg qid for 6 months. | 1995 | 8919168 |