| a review of melioidosis cases in the americas. | melioidosis is a bacterial infection caused by burkholderia pseudomallei, a gram-negative saprophytic bacillus. cases occur sporadically in the americas with an increasing number of cases observed among people with no travel history to endemic countries. to better understand the incidence of the disease in the americas, we reviewed the literature, including unpublished cases reported to the centers for disease control and prevention. of 120 identified human cases, occurring between 1947 and june ... | 2015 | 26458779 |
| emergence of melioidosis in indonesia. | melioidosis is known to be highly endemic in parts of southeast asia and northern australia; however, cases are rarely reported in indonesia. here we report three cases of melioidosis in makassar, south sulawesi, indonesia occurring between 2013 and 2014. two patients died and the other was lost to follow-up. burkholderia pseudomallei isolates from all three cases were identified by the vitek2 compact installed in the hospital in 2012. none of the three patients reported received antimicrobials ... | 2015 | 26458777 |
| evaluation of a biodegradable microparticulate polymer as a carrier for burkholderia pseudomallei subunit vaccines in a mouse model of melioidosis. | melioidosis, a potentially lethal disease of humans and animals, is caused by the soil-dwelling bacterium burkholderia pseudomallei. due to b. pseudomallei's classification as a tier 1 select agent, there is substantial interest in the development of an effective vaccine. yet, despite decades of research, no effective target, adjuvant or delivery vehicle capable of inducing protective immunity against b. pseudomallei infection has been identified. we propose a microparticulate delivery vehicle c ... | 2015 | 26428631 |
| association between activities related to routes of infection and clinical manifestations of melioidosis. | we sought associations between route of infection by burkholderia pseudomallei and clinical manifestations in 330 cases of melioidosis in northeast thailand using bivariate multivariable logistic regression models. activities related to skin inoculation were negatively associated with bacteraemia, activities related to ingestion were associated with bacteraemia, and activities related to inhalation were associated with pneumonia. our study suggests that route of infection is one of the factors r ... | 2016 | 26417852 |
| brief communication genotyping of burkholderia pseudomallei revealed high genetic variability among isolates from a single population group. | burkholderia pseudomallei is a soil dwelling gram-negative bacteria predominates in southeast asia zone and the tropical part of australia. genetic diversity has been explored among various populations and environments worldwide. to date, little data is available on mlst profiling of clinical b. pseudomallei isolates in peninsular malaysia. in this brief report, thirteen culture positive b. pseudomallei cases collected from a single population of terengganu state in the western peninsular malays ... | 2015 | 26417404 |
| burkholderia pseudomallei infection in us traveler returning from mexico, 2014. | | 2015 | 26401597 |
| environmental attributes influencing the distribution of burkholderia pseudomallei in northern australia. | factors responsible for the spatial and temporal clustering of burkholderia pseudomallei in the environment remain to be elucidated. whilst laboratory based experiments have been performed to analyse survival of the organism in various soil types, such approaches are strongly influenced by alterations to the soil micro ecology during soil sanitisation and translocation. during the monsoonal season in townsville, australia, b. pseudomallei is discharged from castle hill (an area with a very high ... | 2015 | 26398904 |
| melioidosis as a travel-associated infection: case report and review of the literature. | increasing numbers of sporadic cases of melioidosis in returning travelers have been reported from non-endemic regions. | 2015 | 26385170 |
| unusual presentation of melioidosis in a case of pseudoaneurysm of descending thoracic aorta: review of two case reports. | melioidosis is a rapidly fatal infectious disease caused by burkholderia pseudomallei, an agent of potential biothreat, endemic in several parts of india. most melioidosis-induced infected aneurysms are located in the abdominal or thoracic aorta. | 2015 | 26380820 |
| synthesis of the tetrasaccharide repeating unit of the β-kdo-containing exopolysaccharide from burkholderia pseudomallei and b. cepacia complex. | the synthesis of the repeating unit of the immunogenic β-kdo-containing exopolysaccharide produced by burkholderia pseudomallei and bacteria of the b. cepacia complex is described. the target tetrasaccharide was synthesized via stereoselective 1,2-cis- and 1,2-trans-galactosylations and β-kdosylation. a [3 + 1] coupling reaction between a trigalactosyl n-phenyl-2,2,2-trifluoroacetimidate donor and a kdo acceptor has been successfully achieved for the assembly of the tetrasaccharide skeleton. | 2015 | 26375291 |
| evaluating the role of phage-shock protein a in burkholderia pseudomallei. | the phage-shock protein (psp) response is an extracytoplasmic response system that is vital for maintenance of the cytoplasmic membrane when the cell encounters stressful conditions. the paradigm of the psp response has been established in escherichia coli. the response has been shown to be important for survival during the stationary phase, maintenance of the proton motive force across membranes and implicated in virulence. in this study, we identified a putative pspa homologue in burkholderia ... | 2015 | 26374246 |
| immune profiling of the progression of a balb/c mouse aerosol infection by burkholderia pseudomallei and the therapeutic implications of targeting hmgb1. | the role of damage-associated molecular pattern hmgb1 signalling in a murine balb/c model of severe respiratory melioidosis (burkholderia pseudomallei infection) was explored in this study. | 2015 | 26358857 |
| inactivation of burkholderia pseudomallei on environmental surfaces using spray-applied, common liquid disinfectants. | five commercially available liquid antimicrobials were evaluated for their ability to decontaminate common environmental surface materials, contaminated with burkholderia pseudomallei, using a spray-based disinfectant delivery procedure. tests were conducted at both an ambient temperature (c. 20°c) and a lower temperature (c. 12°c) condition. nonporous materials (glass and aluminium) were more easily decontaminated than porous materials (wood, concrete and carpet). citric acid (1%) demonstrated ... | 2015 | 26331308 |
| morphological alteration and survival of burkholderia pseudomallei in soil microcosms. | the resilience of burkholderia pseudomallei, the causative agent of melioidosis, was evaluated in control soil microcosms and in soil microcosms containing nacl or feso4 at 30°c. iron (fe(ii)) promoted the growth of b. pseudomallei during the 30-day observation, contrary to the presence of 1.5% and 3% nacl. scanning electron micrographs of b. pseudomallei in soil revealed their morphological alteration from rod to coccoid and the formation of microcolonies. the smallest b. pseudomallei cells wer ... | 2015 | 26324731 |
| the blood transcriptome of experimental melioidosis reflects disease severity and shows considerable similarity with the human disease. | melioidosis, a severe human disease caused by the bacterium burkholderia pseudomallei, has a wide spectrum of clinical manifestations ranging from acute septicemia to chronic localized illness or latent infection. murine models have been widely used to study the pathogenesis of infection and to evaluate novel therapies or vaccines, but how faithfully they recapitulate the biology of human melioidosis at a molecular level is not known. in this study, mice were intranasally infected with either hi ... | 2015 | 26311902 |
| cutaneous melioidosis with unusual histological features. | melioidosis is caused by the saprophytic gram-negative bacillus burkholderia pseudomallei, and has varied presentations, with cutaneous manifestations occurring in about 13% of cases. the usual histopathological features of melioidosis are suppurative to chronic granulomatous inflammation. recommended treatment of melioidosis is sequential use of intravenous followed by oral antibiotics for a few months, although oral antibiotics alone can be used in primary cutaneous melioidosis. we report a ca ... | 2016 | 26299451 |
| genome sequence of a burkholderia pseudomallei clinical isolate from a patient with community-acquired pneumonia and septicemia. | here, we report the draft genome sequence of burkholderia pseudomallei cm_manipal, the causative agent of melioidosis isolated from a diabetic patient in manipal, southern india. the draft genome consists of 107 contigs and is 7,209,157 bp long. a total of 5,600 coding sequences (cdss), 60 trnas, 12 rrnas, and one noncoding rna (ncrna) were predicted from this assembly. | 2015 | 26294629 |
| melioidosis in hainan, china: a restrospective study. | melioidosis is a tropical disease caused by infection with burkholderia pseudomallei. here, we report an 11 year (2002-2013) retrospective study of melioidosis cases in china. | 2015 | 26276499 |
| neuromelioidosis: craniospinal mri findings in burkholderia pseudomallei infection. | to investigate magnetic resonance imaging (mri) findings of central nervous system (cns) infection with burkholderia pseudomallei. | 2017 | 26256582 |
| melioidosis and renal failure in a dutch man after a trip to gambia. | melioidosis is due to burkholderia pseudomallei and is known to be endemic in south-east asia, while epidemiology of disease in sub-saharan africa is still unclear. prompt recognition of infection is crucial for adequate antibiotic treatment. infection can lead to visceral abcesses and awareness of this complication is important for proper management. | 2015 | 26228196 |
| sequence- and structure-based immunoreactive epitope discovery for burkholderia pseudomallei flagellin. | burkholderia pseudomallei is a gram-negative bacterium responsible for melioidosis, a serious and often fatal infectious disease that is poorly controlled by existing treatments. due to its inherent resistance to the major antibiotic classes and its facultative intracellular pathogenicity, an effective vaccine would be extremely desirable, along with appropriate prevention and therapeutic management. one of the main subunit vaccine candidates is flagellin of burkholderia pseudomallei (flicbp). h ... | 2015 | 26222657 |
| melioidosis presenting as a mycotic aneurysm in a korean patient, diagnosed by 16s rrna sequencing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. | a case of melioidosis with a mycotic aneurysm is reported. the blood and tissue isolates were identified as three different species of burkholderia using the automated identification systems, vitek 2 and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms). the isolate was confirmed as burkholderia pseudomallei by 16s rrna sequencing. the typical features of the gram staining of colonies and 16s rrna sequencing can be useful to identify b. pseudomallei. | 2015 | 26216763 |
| neutrophil extracellular traps in the host defense against sepsis induced by burkholderia pseudomallei (melioidosis). | neutrophil extracellular traps (nets) are a central player in the host response to bacteria: neutrophils release extracellular dna (nucleosomes) and neutrophil elastase to entrap and kill bacteria. we studied the role of nets in burkholderia pseudomallei infection (melioidosis), an important cause of gram-negative sepsis in southeast asia. | 2014 | 26215706 |
| burkholderia pseudomallei capsular polysaccharide recognition by a monoclonal antibody reveals key details toward a biodefense vaccine and diagnostics against melioidosis. | burkholderia pseudomallei is the bacterium responsible for melioidosis, an infectious disease with high mortality rates. since melioidosis is a significant public health concern in endemic regions and the organism is currently classified as a potential biothreat agent, the development of effective vaccines and rapid diagnostics is a priority. the capsular polysaccharide (cps) expressed by b. pseudomallei is a highly conserved virulence factor and a protective antigen. because of this, cps is con ... | 2015 | 26198038 |
| comparison of the early host immune response to two widely diverse virulent strains of burkholderia pseudomallei that cause acute or chronic infections in balb/c mice. | burkholderia pseudomallei is the etiologic agent of melioidosis, which is endemic in southeast asia and northern australia. we previously found by the intraperitoneal (ip) route that we could discern differences in virulence in mice amongst different strains of b. pseudomallei. we report an early immune response study comparing two strains in our collection which represent the least, b. pseudomallei 1106a, and one of the most, hbpub10134a, virulent strains in balb/c mice. b. pseudomallei hbpub10 ... | 2015 | 26162294 |
| bacterial reductionism: host thiols enhance virulence. | intracellular bacteria exploit host cytosolic signals to upregulate virulence genes. in this issue of cell host & microbe, wong et al. (2015) show that burkholderia pseudomallei senses host cytosolic glutathione, a low-molecular-weight thiol, through the membrane-bound histidine sensor kinase vira, highlighting the importance of inter-kingdom signaling in bacterial pathogenesis. | 2015 | 26159714 |
| burkholderia pseudomallei survival in lung epithelial cells benefits from mirna-mediated suppression of atg10. | burkholderia pseudomallei is the causative agent of melioidosis, a disease with high mortality, which is prevalent in tropical regions of the world. a recent study shows that b. pseudomallei can survive inside mammalian cells because of its ability to actively evade cell autophagy. however, the underlying mechanisms remain unclear. in the present study, based on microarray screening, we found that atg10 was downregulated following b. pseudomallei infection in a549 human lung epithelial cells. fo ... | 2015 | 26151773 |
| melioidosis cases and selected reports of occupational exposures to burkholderia pseudomallei--united states, 2008-2013. | melioidosis is an infection caused by the gram-negative bacillus burkholderia pseudomallei, which is naturally found in water and soil in areas endemic for melioidosis. infection can be severe and sometimes fatal. the federal select agent program designates b. pseudomallei as a tier 1 overlap select agent, which can affect both humans and animals. identification of b. pseudomallei and all occupational exposures must be reported to the federal select agent program immediately (i.e., within 24 hou ... | 2015 | 26135734 |
| evaluation of polysaccharide-based latex agglutination assays for the rapid detection of antibodies to burkholderia pseudomallei. | melioidosis is a severe disease caused by the gram-negative bacterium burkholderia pseudomallei. diagnosis of melioidosis currently relies on the isolation of b. pseudomallei from clinical samples, which can take several days. an indirect hemagglutination assay (iha) is widely used for serodiagnosis, but it has a short shelf life, is poorly standardized, and requires a viable bacteria culture performed in a biosafety level 3 (bsl-3) laboratory. to improve the diagnostic methods, we have develope ... | 2015 | 26123956 |
| metabolomic profiling of burkholderia pseudomallei using uhplc-esi-q-tof-ms reveals specific biomarkers including 4-methyl-5-thiazoleethanol and unique thiamine degradation pathway. | burkholderia pseudomallei is an emerging pathogen that causes melioidosis, a serious and potentially fatal disease which requires prolonged antibiotics to prevent relapse. however, diagnosis of melioidosis can be difficult, especially in culture-negative cases. while metabolomics represents an uprising tool for studying infectious diseases, there were no reports on its applications to b. pseudomallei. to search for potential specific biomarkers, we compared the metabolomics profiles of culture s ... | 2015 | 26097677 |
| host cytosolic glutathione sensing by a membrane histidine kinase activates the type vi secretion system in an intracellular bacterium. | type vi secretion systems (t6sss) are major virulence mechanisms in many gram-negative bacteria, but the physiological signals that activate them are not well understood. the t6ss1 of burkholderia pseudomallei is essential for pathogenesis in mammalian hosts and is only expressed when the bacterium is intracellular. we found that signals for t6ss1 activation reside in the host cytosol. through site-directed mutagenesis and biochemical studies, we identified low molecular weight thiols, particula ... | 2015 | 26094804 |
| [development and systematical evaluation of an up-converting phosphor technology based lateral flow assay for quantitative detection of burkholderia pseudomallei]. | to develop an up-converting phosphor technology-based lateral-flow (upt-lf) assay for rapid quantitative detection of burkholderia pseudomallei on site. | 2015 | 26081404 |
| control of bacterial metabolism by quorum sensing. | bacterial quorum sensing (qs)-dependent gene expression is a dynamic response to cell density. bacteria produce costly public goods for the benefit of the population as a whole. as an example, qs rewires cellular metabolism to produce oxalate (a public good) to enable survival during the stationary phase in burkholderia glumae, burkholderia thailandensis, and burkholderia pseudomallei. recent reports showed that qs serves as a metabolic brake to maintain homeostatic primary metabolism in b. glum ... | 2015 | 26072043 |
| airborne transmission of melioidosis to humans from environmental aerosols contaminated with b. pseudomallei. | melioidosis results from an infection with the soil-borne pathogen burkholderia pseudomallei, and cases of melioidosis usually cluster after rains or a typhoon. in an endemic area of taiwan, b. pseudomallei is primarily geographically distributed in cropped fields in the northwest of this area, whereas melioidosis cases are distributed in a densely populated district in the southeast. we hypothesized that contaminated cropped fields generated aerosols contaminated with b. pseudomallei, which wer ... | 2015 | 26061639 |
| a preliminary x-ray study of 3-deoxy-d-manno-oct-2-ulosonic acid 8-phosphate phosphatase (yrbi) from burkholderia pseudomallei. | 3-deoxy-d-manno-oct-2-ulosonic acid 8-phosphate phosphatase (yrbi), the third enzyme in the pathway for the biosynthesis of 3-deoxy-d-manno-oct-2-ulosonic acid (kdo), hydrolyzes kdo 8-phosphate to kdo and inorganic phosphate. yrbi belongs to the haloacid dehalogenase (had) superfamily, which is a large family of magnesium-dependent phosphatase/phosphotransferase enzymes. in this study, yrbi from burkholderia pseudomallei, the causative agent of melioidosis, has been cloned, expressed, purified a ... | 2015 | 26057814 |
| rapid diagnostics for melioidosis: a comparative study of a novel lateral flow antigen detection assay. | the rapid diagnosis of septicaemic melioidosis will have an impact on reduction of mortality. currently, this relies almost exclusively upon culture of the causative agent burkholderia pseudomallei from clinical samples. in acute sepsis, blood is the preferred specimen for culture and therefore should be the target for a rapid diagnostic tool. a lateral flow immunoassay (lfi) for the detection of b. pseudomallei antigen has been developed. this was compared with molecular detection using the tar ... | 2015 | 26055557 |
| "melioidosis in antioquia, colombia: an emerging or endemic disease? a cases series". | melioidosis is endemic in malaysia, the southwest of thailand, and northern australia. the incidence in thailand is 4.4/1000000 inhabitants, where it causes 19% of community-acquired pneumonia (cap) and 20% of bacteremic pneumonia, and the mortality is 50%. sporadic cases have been described in central and south america. the objective of this study was to describe the clinical and epidemiological features and ecological characteristics of melioidosis in antioquia, colombia. | 2015 | 26051974 |
| direct detection of burkholderia pseudomallei and biological factors in soil. | burkholderia pseudomallei, a gram-negative saprophytic bacillus, is a severe infectious agent that causes melioidosis and soil is the most important reservoir. | 2015 | 26048871 |
| burkholderia pseudomallei in soil samples from an oceanarium in hong kong detected using a sensitive pcr assay. | melioidosis, caused by burkholderia pseudomallei, is an emerging infectious disease with an expanding geographical distribution. although assessment of the environmental load of b. pseudomallei is important for risk assessment in humans or animals in endemic areas, traditional methods of bacterial culture for isolation have low sensitivities and are labor-intensive. using a specific polymerase chain reaction (pcr) assay targeting a tat domain protein in comparison with a bacterial culture method ... | 2014 | 26038496 |
| melioidosis mycotic aneurysm: an uncommon complication of an uncommon disease. | melioidosis is often considered an exotic and uncommon disease in most parts of the world. however it is an endemic disease in southeast asia and northern australia with an expanding distribution. melioidosis can involve almost any organ and can deteriorate rapidly. in this report, we describe a rapidly fatal case of a mycotic aneurysm associated with melioidosis despite aggressive antibiotic therapy. the morbidity and mortality of this uncommon complication remains high despite prompt diagnosis ... | 2015 | 26029577 |
| macroautophagy is essential for killing of intracellular burkholderia pseudomallei in human neutrophils. | neutrophils play a key role in the control of burkholderia pseudomallei, the pathogen that causes melioidosis. here, we show that survival of intracellular b. pseudomallei was significantly increased in the presence of 3-methyladenine or lysosomal cathepsin inhibitors. the lc3-flux was increased in b. pseudomallei-infected neutrophils. concordant with this result, confocal microscopy analyses using anti-lc3 antibodies revealed that b. pseudomallei-containing phagosomes partially overlapped with ... | 2015 | 25996656 |
| consensus on the development of vaccines against naturally acquired melioidosis. | several candidates for a vaccine against burkholderia pseudomallei, the causal bacterium of melioidosis, have been developed, and a rational approach is now needed to select and advance candidates for testing in relevant nonhuman primate models and in human clinical trials. development of such a vaccine was the topic of a meeting in the united kingdom in march 2014 attended by international candidate vaccine developers, researchers, and government health officials. the focus of the meeting was a ... | 2015 | 25992835 |
| endemic melioidosis in residents of desert region after atypically intense rainfall in central australia, 2011. | after heavy rains and flooding during early 2011 in the normally arid interior of australia, melioidosis was diagnosed in 6 persons over a 4-month period. although the precise global distribution of the causal bacterium burkholderia pseudomallei remains to be determined, this organism can clearly survive in harsh and even desert environments outside the wet tropics. | 2015 | 25988301 |
| backbone chemical shift assignments for the sensor domain of the burkholderia pseudomallei histidine kinase riss: "missing" resonances at the dimer interface. | using a deuterated sample, all the observable backbone (1)h(n), (15)n, (13)c(a), and (13)c' chemical shifts for the dimeric, periplasmic sensor domain of the burkholderia pseudomallei histidine kinase riss were assigned. approximately one-fifth of the amide resonances are "missing" in the (1)h-(15)n hsqc spectrum and map primarily onto α-helices at the dimer interface observed in a crystal structure suggesting this region either undergoes intermediate timescale motion (μs-ms) and/or is heterogen ... | 2015 | 25957069 |
| melioidosis as a cause of acute abdomen in immuno-competent male from eastern india. | though melioidosis is rare in india, it has gained importance as one of the most potent emerging infections. in india, the cases have been under-reported because of the lack of awareness. the majority of cases present with multifocal pyogenic infections with septicemia. we present an unusual case of melioidosis presenting as acute intestinal perforation. the organism was ceftazidime resistant, and we successfully treated the case with imipenem and doxycyclin. this case highlights ruling out the ... | 2017 | 25949062 |
| case of a lung mass due to melioidosis in mexico. | melioidosis, an infection caused by the gram-negative bacterium burkholderia pseudomallei, is an important cause of pneumonia, skin infection, sepsis, and death in southeast asia and australia, but is exceedingly rare in north america. pulmonary melioidosis typically presents as acute bacterial pneumonia or cavitary lung lesions resembling tuberculosis. | 2015 | 25943405 |
| whole-genome sequence of an epidemic strain of burkholderia pseudomallei vgh07 in taiwan. | here, we report the complete genome sequence of b. pseudomallei vgh07. this is an epidemic strain that was isolated from a melioidosis patient with arthro-osteomyelitis in taiwan. | 2015 | 25931599 |
| curcumin rescues caenorhabditis elegans from a burkholderia pseudomallei infection. | the tropical pathogen burkholderia pseudomallei requires long-term parenteral antimicrobial treatment to eradicate the pathogen from an infected patient. however, the development of antibiotic resistance is emerging as a threat to this form of treatment. to meet the need for alternative therapeutics, we proposed a screen of natural products for compounds that do not kill the pathogen, but in turn, abrogate bacterial virulence. we suggest that the use of molecules or compounds that are non-bacter ... | 2015 | 25914690 |
| effects of colonization of the roots of domestic rice (oryza sativa l. cv. amaroo) by burkholderia pseudomallei. | burkholderia pseudomallei is a saprophytic bacterium that causes melioidosis and is often isolated from rice fields in southeast asia, where the infection incidence is high among rice field workers. the aim of this study was to investigate the relationship between this bacterium and rice through growth experiments where the effect of colonization of domestic rice (oryza sativa l. cv amaroo) roots by b. pseudomallei could be observed. when b. pseudomallei was exposed to surface-sterilized seeds, ... | 2015 | 25911477 |
| melioidosis in trinidad and tobago. | | 2015 | 25897877 |
| backbone and side-chain (1)h, (15)n, (13)c assignment and secondary structure of bpsl1445 from burkholderia pseudomallei. | bpsl1445 is a lipoprotein produced by the gram-negative bacterium burkholderia pseudomallei (b. pseudomallei), the etiological agent of melioidosis. immunodetection assays against sera patients using protein microarray suggest bpsl1445 involvement in melioidosis. herein we report backbone, side chain nmr assignment and secondary structure for the recombinant protein. | 2015 | 25893672 |
| case-control investigation on the risk factors of melioidosis in small ruminant farms in peninsular malaysia. | epidemiology of melioidosis is poorly understood because its occurrence is influenced by complex interaction of environmental, climatic, physicochemical and host factors. we investigated the potential risk factors for the exposure to burkholderia pseudomallei in small ruminants' farms in peninsular malaysia. | 2015 | 25891038 |
| whole-genome sequences of five burkholderia pseudomallei isolates from australian cystic fibrosis patients. | we report here five improved high-quality draft genomes of burkholderia pseudomallei isolated from australian cystic fibrosis (cf) patients. this pathogen is rarely seen in cf patients. these genomes will be used to better understand chronic carriage of b. pseudomallei in the cf lung and the within-host evolution of longitudinal isolates from these patients. | 2015 | 25883282 |
| splenic abscesses in a returning traveler. | burkholderia, an aerobic gram-negative rod, is the causative organism behind melioidosis and is a common soil and water organism found predominantly in south-east asia. we report the case of a 68 year-old man returning from an extended trip to the philippines, with splenic hypodense lesions on abdominal computer tomography scan, later confirmed to be culture-positive for burkholderia pseudomallei. the patient was treated with a course of intravenous ceftazidime followed by eradication therapy wi ... | 2015 | 25874071 |
| intracoronary blood sampling with a microcatheter for the diagnosis of giant infective coronary aneurysm: melioidosis of coronary artery mycotic aneurysm. | | 2015 | 25863294 |
| virulent burkholderia species mimic host actin polymerases to drive actin-based motility. | burkholderia pseudomallei and b. mallei are bacterial pathogens that cause melioidosis and glanders, whereas their close relative b. thailandensis is non-pathogenic. all use the trimeric autotransporter bima to facilitate actin-based motility, host cell fusion, and dissemination. here, we show that bima orthologs mimic different host actin-polymerizing proteins. b. thailandensis bima activates the host arp2/3 complex. in contrast, b. pseudomallei and b. mallei bima mimic host ena/vasp actin poly ... | 2015 | 25860613 |
| exploiting molecular virulence determinants in burkholderia to develop vaccine antigens. | the burkholderia genus is a highly diverse group of species that are distributed throughout a wide range of environments and habitats. among this group, which is remarkable for its adaptability to a wider range of environmental conditions including disinfectants and organic solvents, are a subgroup that represents some of the most difficult to treat infections. this subgroup includes burkholderia pseudomallei, the causative agent of melioidosis; b. mallei, the causative agent of glanders and b. ... | 2015 | 25850766 |
| burkholderia pseudomallei sequencing identifies genomic clades with distinct recombination, accessory, and epigenetic profiles. | | 2015 | 25834186 |
| correction: interrogation of the burkholderia pseudomallei genome to address differential virulence among isolates. | [this corrects the article doi: 10.1371/journal.pone.0115951.]. | 2015 | 25806507 |
| what drives the occurrence of the melioidosis bacterium burkholderia pseudomallei in domestic gardens? | melioidosis is an often fatal infectious disease affecting humans and animals in tropical regions and is caused by the saprophytic environmental bacterium burkholderia pseudomallei. domestic gardens are not only a common source of exposure to soil and thus to b. pseudomallei, but they also have been found to contain more b. pseudomallei than other environments. in this study we addressed whether anthropogenic manipulations common to gardens such as irrigation or fertilizers change the occurrence ... | 2015 | 25803046 |
| burkholderia pseudomallei in cystic fibrosis and treatment complications. | a healthy 29-year-old australian man with cystic fibrosis (cf) grew b urkholderia pseudomallei on a routine sputum culture 1 month after returning from holiday in thailand. he underwent a 12-month treatment regime with multiple antibiotics resulting in a number of adverse events. sputum cultures were cleared of the pathogen and remain negative 8 years post-treatment. there were no clinical sequelae and no deterioration in lung function. few reports have been published to date on melioidosis in c ... | 2015 | 25802738 |
| programmed death ligand 1 on burkholderia pseudomallei-infected human polymorphonuclear neutrophils impairs t cell functions. | polymorphonuclear neutrophils (pmns) are terminally differentiated cells that are involved in innate immune responses and form an early line of defense against pathogens. more recently, it has been shown that pmns have immunosuppressive abilities on other immune cells. however, the effect of pmns on t cell responses during bacterial infection remains to be determined. in this report, we examined the interaction of pmns and t cells in response to infection with burkholderia pseudomallei, the caus ... | 2015 | 25801435 |
| regulatory role of gsk3β in the activation of nf-κb and modulation of cytokine levels in burkholderia pseudomallei-infected pbmc isolated from streptozotocin-induced diabetic animals. | increased susceptibility of diabetics to melioidosis, a disease caused by the burkholderia pseudomallei bacterium is believed to be attributed to dysfunction of the innate immune system. however, the underlying mechanism of the innate susceptibility is not well-understood. glycogen synthase kinase-3β (gsk3β) plays an important role in the innate inflammatory response caused by bacterial pathogens. the present study was conducted to investigate the effects of gsk3β inhibition by licl on levels of ... | 2015 | 25801253 |
| colony morphology variation of burkholderia pseudomallei is associated with antigenic variation and o-polysaccharide modification. | burkholderia pseudomallei is a cdc tier 1 select agent that causes melioidosis, a severe disease in humans and animals. persistent infections are common, and there is currently no vaccine available. lipopolysaccharide (lps) is a potential vaccine candidate. b. pseudomallei expresses three serologically distinct lps types. the predominant o-polysaccharide (ops) is an unbranched heteropolymer with repeating d-glucose and 6-deoxy-l-talose residues in which the 6-deoxy-l-talose residues are variably ... | 2015 | 25776750 |
| melioidosis in children: a retrospective study. | melioidosis, caused by burkholderia pseudomallei, is endemic in singapore and can present as localized or disseminated disease. | 2015 | 25771733 |
| thalassemia major is a major risk factor for pediatric melioidosis in kota kinabalu, sabah, malaysia. | melioidosis is an important cause of community-acquired infection in southeast asia and northern australia. studies from endemic countries have demonstrated differences in the epidemiology and clinical features among children diagnosed with melioidosis. this suggests that local data are needed to determine the risk factors and outcome in specific areas. | 2015 | 25767257 |
| trimethoprim/sulfamethoxazole resistance in clinical isolates of burkholderia pseudomallei from thailand. | | 2015 | 25758020 |
| melioidosis presenting as fever and jaundice: a rare presentation. | melioidosis caused by the environmental gram-negative bacillus burkholderia pseudomallei is endemic in northern australia and southeast asia and is being described increasingly from south and west coastal regions of india. melioidosis is known to have high mortality (14-50%) and the risk factors associated with it are diabetes mellitus and heavy alcohol abuse. melioidosis primarily presents as pneumonia, genitourinary infection and bacteremia. we present a case of melioidosis from north india, a ... | 2014 | 25755553 |
| parietal bone osteomyelitis in melioidosis. | we report a case of a 55-year-old man with uncontrolled diabetes who presented with pneumonia. during his hospital stay his clinical status worsened and he had a focal seizure. mri showed central nervous system involvement and parietal bone osteomyelitis. as the patient's blood culture and endotracheal aspirate grew burkholderia pseudomallei, melioidosis was diagnosed. he was treated with meropenem after failure to respond to ceftazidime. he gradually improved over a period of 4 weeks and was di ... | 2015 | 25725029 |
| detection of burkholderia pseudomallei in sputum using selective enrichment broth and ashdown's medium at kampong cham provincial hospital, cambodia. | melioidosis, infection caused by burkholderia pseudomallei, is increasingly reported in cambodia. we hypothesized that implementation of an enhanced sputum testing protocol in a provincial hospital diagnostic microbiology laboratory would increase detection of b. pseudomallei. we tested 241 sputum specimens that were deemed acceptable for culture, comparing culture in selective enrichment broth followed by sub-culture on ashdown's medium to standard culture methods. two specimens (0.8%) were pos ... | 2014 | 25717370 |
| melioidosis in africa: should we be looking more closely? | melioidosis is a life-threatening infection caused by the gram-negative bacterium burkholderia pseudomallei, mainly found in southeast asia. recently, african foci have been identified, although reports remain mostly anecdotal. in africa, multiple febrile diseases have been erroneously attributed to malaria in the past, and many cases of fever remain mis- or undiagnosed. vigilance for previously under-recognized pathogens may enhance our understanding of disease epidemiology and facilitate impro ... | 2015 | 25689538 |
| whole-genome sequences of 80 environmental and clinical isolates of burkholderia pseudomallei. | here, we present the draft genome sequences of 80 isolates of burkholderia pseudomallei. the isolates represent clinical cases of melioidosis and environmental isolates from regions in australia and papua new guinea where b. pseudomallei is endemic. the genomes provide further context for the diversity of the pathogen. | 2015 | 25676747 |
| septicaemic melioidosis: case report from a non-endemic area. | melioidosis is a clinically diverse disease caused by the facultative intracellular gram-negative bacterium, burkholderia pseudomallei. in recent times melioidosis has been increasingly reported in india especially from the southern and coastal states. we report a fatal case of septicaemic melioidosis from the state of rajasthan with a view to increase awareness about the existence of this disease in an area yet unrecognized. | 2014 | 25653950 |
| a burkholderia pseudomallei colony variant necessary for gastric colonization. | diverse colony morphologies are a hallmark of burkholderia pseudomallei recovered from infected patients. we observed that stresses that inhibit aerobic respiration shifted populations of b. pseudomallei from the canonical white colony morphotype toward two distinct, reversible, yet relatively stable yellow colony variants (ya and yb). as accumulating evidence supports the importance of b. pseudomallei enteric infection and gastric colonization, we tested the response of yellow variants to hypox ... | 2015 | 25650400 |
| extended loop region of hcp1 is critical for the assembly and function of type vi secretion system in burkholderia pseudomallei. | the type vi secretion system cluster 1 (t6ss1) is essential for the pathogenesis of burkholderia pseudomallei, the causative agent of melioidosis, a disease endemic in the tropics. inside host cells, b. pseudomallei escapes into the cytosol and through t6ss1, induces multinucleated giant cell (mngc) formation that is thought to be important for bacterial cell to cell spread. the hemolysin-coregulated protein (hcp) is both a t6ss substrate, as well as postulated to form part of the t6ss secretion ... | 2015 | 25648885 |
| from crystal structure to in silico epitope discovery in the burkholderia pseudomallei flagellar hook-associated protein flgk. | melioidosis, caused by the gram-negative bacterium burkholderia pseudomallei, is a potentially fatal infection that is endemic in southeast asia and northern australia that is poorly controlled by antibiotics. research efforts to identify antigenic components for a melioidosis vaccine have led to the identification of several proteins, including subunits forming the flagella that mediate bacterial motility, host colonization, and virulence. this study focuses on the b. pseudomallei flagellar hoo ... | 2015 | 25645451 |
| afn-1252 is a potent inhibitor of enoyl-acp reductase from burkholderia pseudomallei--crystal structure, mode of action, and biological activity. | melioidosis is a tropical bacterial infection caused by burkholderia pseudomallei (b. pseudomallei; bpm), a gram-negative bacterium. current therapeutic options are largely limited to trimethoprim-sulfamethoxazole and β-lactam drugs, and the treatment duration is about 4 months. moreover, resistance has been reported to these drugs. hence, there is a pressing need to develop new antibiotics for melioidosis. inhibition of enoyl-acp reducatase (fabi), a key enzyme in the fatty acid biosynthesis pa ... | 2015 | 25644789 |
| melioidosis: evolving concepts in epidemiology, pathogenesis, and treatment. | infection with burkholderia pseudomallei can result in asymptomatic seroconversion, a single skin lesion that may or may not heal spontaneously, a pneumonia which can be subacute or chronic and mimic tuberculosis or rapidly progressive resulting in fatal overwhelming sepsis. latency with subsequent activation of disease is well recognized, but very uncommon. melioidosis also has a myriad of other clinical presentations and diagnosis is often delayed because of this and because of difficulties wi ... | 2015 | 25643275 |
| quantitative proteomic analysis of burkholderia pseudomallei bsa type iii secretion system effectors using hypersecreting mutants. | burkholderia pseudomallei is an intracellular pathogen and the causative agent of melioidosis, a severe disease of humans and animals. one of the virulence factors critical for early stages of infection is the burkholderia secretion apparatus (bsa) type 3 secretion system (t3ss), a molecular syringe that injects bacterial proteins, called effectors, into eukaryotic cells where they subvert cellular functions to the benefit of the bacteria. although the bsa t3ss itself is known to be important fo ... | 2015 | 25635268 |
| tracing melioidosis back to the source: using whole-genome sequencing to investigate an outbreak originating from a contaminated domestic water supply. | melioidosis, a disease of public health importance in southeast asia and northern australia, is caused by the gram-negative soil bacillus burkholderia pseudomallei. melioidosis is typically acquired through environmental exposure, and case clusters are rare, even in regions where the disease is endemic. b. pseudomallei is classed as a tier 1 select agent by the centers for disease control and prevention; from a biodefense perspective, source attribution is vital in an outbreak scenario to rule o ... | 2015 | 25631791 |
| transcription level analysis of intracellular burkholderia pseudomallei illustrates the role of bpsl1502 during bacterial interaction with human lung epithelial cells. | melioidosis caused by burkholderia pseudomallei is a globally important disease of increasing concern according to high case-fatality rate and epidemic spreading. the ability of b. pseudomallei to attach and invade host cells and subsequently survive intracellularly has stimulated many questions concerning the comprehension of bacterial pathogenesis progression. transcription levels of intracellular b. pseudomallei genes in human lung epithelial cells were therefore analyzed using bioinformatic ... | 2015 | 25626369 |
| pleuropulmonary melioidosis with osteomyelitis rib. | melioidosis is a multiorgan infectious disease caused by burkholderia pseudomallei. few cases have been reported from south india. this is a case report of pleuropulmonary melioidosis with rib osteomyelitis. | 2017 | 25624602 |
| a prospective study of melioidosis after environmental exposure of healthy participants to burkholderia pseudomallei during a muddy endurance challenge. | in a prospective study of 123 healthy adults competing in a mud-exposing endurance challenge in the melioidosis-endemic tropical north of the northern territory of australia, there were no asymptomatic seroconversions to burkholderia pseudomallei using indirect hemagglutination assay. however, one competitor developed melioidosis attributable to infection acquired during the event. | 2015 | 25624406 |
| transcriptome analysis of burkholderia pseudomallei t6ss identifies hcp1 as a potential serodiagnostic marker. | burkholderia pseudomallei, the causative agent of melioidosis, is able to survive extreme environments and utilizes various virulence factors for survival and pathogenicity. to compete and survive within these different ecological niches, b. pseudomallei has evolved specialized pathways, including the type vi secretion systems (t6sss), that have a role in pathogenesis as well as interbacterial interactions. we examined the expression profile of b. pseudomallei t6ss six gene clusters during infec ... | 2015 | 25616255 |
| burkholderia pseudomallei type iii secretion system cluster 3 atpase bsas, a chemotherapeutic target for small-molecule atpase inhibitors. | melioidosis is an infectious disease of high mortality for humans and other animal species; it is prevalent in tropical regions worldwide. the pathogenesis of melioidosis depends on the ability of its causative agent, the gram-negative bacterium burkholderia pseudomallei, to enter and survive in host cells. b. pseudomallei can escape from the phagosome into the cytosol of phagocytic cells where it replicates and acquires actin-mediated motility, avoiding killing by the autophagy-dependent proces ... | 2015 | 25605762 |
| the first autochthonous case of human melioidosis in reunion island. | | 2015 | 25596804 |
| cutaneous melioidosis in a healthy danish man after travelling to south-east asia. | a healthy danish man presented with infected prepatellar bursitis 8 months after being involved in a car accident in malaysia resulting in exposure of a laceration of his knee to stagnant water. tissue samples grew burkholderia pseudomallei and diagnostic work up revealed no secondary foci. the patient was successfully treated with surgical debridement and 3 months of oral trimethoprim-sulfamethoxazole. at 6 months follow-up the patient was without relapse. | 2015 | 25596295 |
| retrospective study on fatal melioidosis in captive zoo animals in thailand. | melioidosis is caused by burkholderia pseudomallei and is an important zoonotic infectious disease causing high mortality from fulminant septicaemia in humans and a wide variety of animal species. the incidence of fatal melioidosis in zoo animals has been significant in many thai zoos. a total number of 32 cases were evaluated throughout the thai zoo animal populations. the highest prevalence of disease has been reported from the north-eastern region followed by the zoos in the southern part of ... | 2016 | 25583276 |
| novel phospholipase a2 inhibitors from python serum are potent peptide antibiotics. | antimicrobial peptides (amps) play a vital role in defense against resistant bacteria. in this study, eight different amps synthesized from python reticulatus serum protein were tested for bactericidal activity against various gram-positive and gram-negative bacteria (staphylococcus aureus, burkholderia pseudomallei (khw and tes strains), and proteus vulgaris) using a disc-diffusion method (20 μg/disc). among the tested peptides, phospholipase a2 inhibitory peptide (pip)-18[59-76], β-asp65-pip[5 ... | 2015 | 25583073 |
| type 3 secretion system cluster 3 is a critical virulence determinant for lung-specific melioidosis. | burkholderia pseudomallei, the bacterial agent of melioidosis, causes disease through inhalation of infectious particles, and is classified as a tier 1 select agent. optical diagnostic imaging has demonstrated that murine respiratory disease models are subject to significant upper respiratory tract (urt) colonization. because human melioidosis is not associated with urt colonization as a prominent presentation, we hypothesized that lung-specific delivery of b. pseudomallei may enhance our abilit ... | 2015 | 25569630 |
| interrogation of the burkholderia pseudomallei genome to address differential virulence among isolates. | infection by the gram-negative pathogen burkholderia pseudomallei results in the disease melioidosis, acquired from the environment in parts of southeast asia and northern australia. clinical symptoms of melioidosis range from acute (fever, pneumonia, septicemia, and localized infection) to chronic (abscesses in various organs and tissues, most commonly occurring in the lungs, liver, spleen, kidney, prostate and skeletal muscle), and persistent infections in humans are difficult to cure. underst ... | 2014 | 25536074 |
| plasmacytoid dendritic cell bactericidal activity against burkholderia pseudomallei. | melioidosis sepsis, caused by burkholderia pseudomallei, is associated with high mortality due to an overwhelming inflammatory response. plasmacytoid dendritic cells (pdc) are potent producers of type i interferons (ifn). this study investigated whether pdc and type i ifn play a role during the early stages of b. pseudomallei infection. human and murine pdc internalised and killed b. pseudomallei as efficiently as murine conventional dc (cdc). pdc derived from b. pseudomallei-susceptible (balb/c ... | 2015 | 25532693 |
| burkholderia pseudomallei sequence type 562 in china and australia. | | 2015 | 25531912 |
| association of melioidosis incidence with rainfall and humidity, singapore, 2003-2012. | soil has been considered the natural reservoir for the bacterium burkholderia pseudomallei, which causes melioidosis. we examined 550 melioidosis cases that occurred during a 10-year period in the highly urbanized city of singapore, where soil exposure is rare, and found that rainfall and humidity levels were associated with disease incidence. | 2015 | 25531547 |
| neurotropic threat characterization of burkholderia pseudomallei strains. | the death rate for neurologic melioidosis is high. whether certain burkholderia pseudomallei strains are more likely than other strains to cause central nervous system infection and whether route of infection influences the neurotropic threat remain unclear. therefore, we compared the virulence and dissemination of australian clinical isolates collected during october 1989-october 2012 from patients with neurologic and nonneurologic melioidosis after intranasal and subcutaneous infection of mice ... | 2015 | 25530166 |
| clinical, environmental, and serologic surveillance studies of melioidosis in gabon, 2012-2013. | burkholderia pseudomallei, an environmental gram-negative bacillus, is the causative agent of melioidosis and a bio-threat agent. reports of b. pseudomallei isolation from soil and animals in east and west africa suggest that melioidosis might be more widely distributed than previously thought. because it has been found in equatorial areas with tropical climates, we hypothesized that b. pseudomallei could exist in gabon. during 2012-2013, we conducted a seroprevalance study in which we set up mi ... | 2015 | 25530077 |
| structure-based design of a b cell antigen from b. pseudomallei. | burkholderia pseudomallei is the etiological agent of melioidosis, a severe endemic disease in south-east asia, causing septicemia and organ failure with high mortality rates. current treatments and diagnostic approaches are largely ineffective. the development of new diagnostic tools and vaccines toward effective therapeutic opportunities against b. pseudomallei is therefore an urgent priority. in the framework of a multidisciplinary project tackling melioidosis through reverse and structural v ... | 2015 | 25495888 |
| cloning, expression and purification of outer membrane protein (ompa) of burkholderia pseudomallei and evaluation of its potential for serodiagnosis of melioidosis. | melioidosis is an emerging infectious disease in india and caused by gram-negative, soil saprophyte bacteria burkholderia pseudomallei. this disease is endemic in southeast asia and northern australia, and sporadic cases of melioidosis are also reported from southern states of india. the present study reports the cloning, expression, and purification of recombinant protein outer membrane protein a (ompa) of b. pseudomallei and its evaluation in indirect enzyme-linked immunosorbent assay (elisa) ... | 2015 | 25488273 |
| seroepidemiology of melioidosis in children from a remote region of papua new guinea. | the balimo region in papua new guinea has previously been identified as melioidosis-endemic with a predilection for children. where health resources are scarce, seroepidemiology can be used to assess exposure to burkholderia pseudomallei and therefore risk of acquiring melioidosis. | 2015 | 25487725 |
| crystallization and preliminary crystallographic studies of the hypothetical protein bpsl1038 from burkholderia pseudomallei. | melioidosis is an infectious disease caused by the pathogenic bacterium burkholderia pseudomallei. whole-genome sequencing revealed that the b. pseudomallei genome includes 5855 coding dna sequences (cdss), of which ∼25% encode hypothetical proteins. a pathogen-associated hypothetical protein, bpsl1038, was overexpressed in escherichia coli, purified and crystallized using vapour-diffusion methods. a bpsl1038 protein crystal that grew using sodium formate as precipitant diffracted to 1.55 å reso ... | 2014 | 25484229 |