Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| transient in vivo resistance mechanisms of burkholderia pseudomallei to ceftazidime and molecular markers for monitoring treatment response. | much is known about the mode of action of drugs and resistance mechanisms under laboratory growth conditions, but research on the bacterial transcriptional response to drug pressure in vivo or efficacious mode of action and transient resistance mechanisms of clinically employed drugs is limited. accordingly, to assess active alternative metabolism and transient resistance mechanisms, and identify molecular markers of treatment response, the in vivo transcriptional response of burkholderia pseudo ... | 2017 | 28081127 |
| upconversion nanocrystals mediated lateral-flow nanoplatform for in vitro detection. | upconversion phosphors (ucps) that are free from interference from biological sample autofluorescence have attracted attention for in vivo and in vitro bioapplications. however, ucps need to be water-dispersible, nanosized, and highly luminous to realize broad applications. therefore, the aim of this research is to develop ucps that meet these comprehensive criteria for in vitro diagnosis. to combine nano size with high luminous intensity, β-nayf4:yb(3+),er(3+) upconversion nanocrystals (ucnps) ... | 2017 | 28067495 |
| boosting of post-exposure human t-cell and b-cell recall responses in vivo by burkholderia pseudomallei-related proteins. | burkholderia pseudomallei is the causative agent of melioidosis, an infectious disease with high incidence and mortality in south east asia and northern australia. to date there is no protective vaccine and antibiotic treatment is prolonged and not always effective. most people living in endemic areas have been exposed to the bacteria and have developed some immunity, which may have helped to prevent disease. here, we used a humanized mouse model (hu-pbl-scid), reconstituted with human periphera ... | 2017 | 28066900 |
| assessing the potential for burkholderia pseudomallei in the southeastern united states. | 2017 | 28058957 | |
| secondary metabolites from bacillus amyloliquefaciens isolated from soil can kill burkholderia pseudomallei. | bacillus species are gram-positive bacteria found in abundance in nature and their secondary metabolites were found to possess various potential activities, notably antimicrobial. in this study, bacillus amyloliquefaciens n2-4 and n3-8 were isolated from soil and their metabolites could kill burkholderia pseudomallei, a gram-negative pathogenic bacterium also found in soil in its endemic areas. moreover, the metabolites were able to kill drug resistant isolates of b. pseudomallei and also inhibi ... | 2017 | 28050857 |
| survival and intra-nuclear trafficking of burkholderia pseudomallei: strategies of evasion from immune surveillance? | during infection, successful bacterial clearance is achieved via the host immune system acting in conjunction with appropriate antibiotic therapy. however, it still remains a tip of the iceberg as to where persistent pathogens namely, burkholderia pseudomallei (b. pseudomallei) reside/hide to escape from host immune sensors and antimicrobial pressure. | 2017 | 28045926 |
| imported case of melioidosis in oman: case report. | melioidosis is an infectious disease caused by the bacterium burkholderia pseudomallei. it is most commonly described in southeast asia and northern australia and some imported cases in non-endemic areas. we describe the case of a 55-year-old omani man with fulminant sepsis who worked in laos, cambodia. b. pseudomallei was isolated from the patient's blood and was identified by means of microbiological and biochemical tests. we highlight the importance of careful attention to non-fermentative gr ... | 2017 | 28042405 |
| finafloxacin overcomes burkholderia pseudomallei efflux-mediated fluoroquinolone resistance. | 2017 | 28039270 | |
| burkholderia pseudomallei type iii secreted protein bipc: role in actin modulation and translocation activities required for the bacterial intracellular lifecycle. | melioidosis, an infection caused by the facultative intracellular pathogen burkholderia pseudomallei, has been classified as an emerging disease with the number of patients steadily increasing at an alarming rate. b. pseudomalleipossess various virulence determinants that allow them to invade the host and evade the host immune response, such as the type iii secretion systems (ttss). the products of this specialized secretion system are particularly important for the b. pseudomallei infection. la ... | 2016 | 28028452 |
| sulfonamide inhibition profile of the γ-carbonic anhydrase identified in the genome of the pathogenic bacterium burkholderia pseudomallei the etiological agent responsible of melioidosis. | a new γ-carbonic anhydrase (ca, ec 4.1.1.1) was cloned and characterized kinetically in the genome of the bacterial pathogen burkholderia pseudomallei, the etiological agent of melioidosis, an endemic disease of tropical and sub-tropical regions of the world. the catalytic activity of this new enzyme, bpscaγ, is significant with a kcat of 5.3×10(5)s(-1) and kcat/km of 2.5×10(7)m(-1)×s(-1) for the physiologic co2 hydration reaction. the inhibition constant value for this enzyme for 39 sulfonamide ... | 2017 | 28025002 |
| evolution of bopa gene in burkholderia: a case of convergent evolution as a mechanism for bacterial autophagy evasion. | autophagy is an important defense mechanism targeting intracellular bacteria to restrict their survival and growth. on the other hand, several intracellular pathogens have developed an antiautophagy mechanism to facilitate their own replication or intracellular survival. up to now, no information about the origin or evolution of the antiautophagic genes in bacteria is available. bopa is an effector protein secreted by burkholderia pseudomallei via the type three secretion system, and it has been ... | 2016 | 28018913 |
| a predicted cation transporter protein, bpss1228, is involved in intracellular behaviour of burkholderia pseudomallei in a human lung epithelial cell line (a549). | burkholderia pseudomallei causes melioidosis, a potentially fatal infectious disease in tropical and subtropical countries worldwide. the intracellular behaviour of this pathogen in host cells has been reported to impact the severity of melioidosis, including the development of septicaemia, a consequence of pneumonia melioidosis. we previously identified a predicted cation transporter protein, bpss1228, that participates in the transitional stage of this intracellular pathogen. for further analy ... | 2016 | 28003338 |
| non-caseating granulomatous infective spondylitis: melioidotic spondylitis. | retrospective clinical analysis. | 2016 | 27994782 |
| development of immunoassays for burkholderia pseudomallei typical and atypical lipopolysaccharide strain typing. | burkholderia pseudomallei is the causative agent of melioidosis, a severe infection endemic to many tropical regions. lipopolysaccharide (lps) is recognized as an important virulence factor used by b. pseudomallei isolates of b. pseudomallei have been shown to express one of four different types of lps (typical lps, atypical lps types b and b2, and rough lps) and in vitro studies have demonstrated that lps types may impact disease severity. the association between lps types and clinical manifest ... | 2017 | 27994103 |
| unravelling the molecular epidemiology and genetic diversity among burkholderia pseudomallei isolates from south india using multi-locus sequence typing. | there is a slow but steady rise in the case detection rates of melioidosis from various parts of the indian sub-continent in the past two decades. however, the epidemiology of the disease in india and the surrounding south asian countries remains far from well elucidated. multi-locus sequence typing (mlst) is a useful epidemiological tool to study the genetic relatedness of bacterial isolates both with-in and across the countries. with this background, we studied the molecular epidemiology of 32 ... | 2016 | 27992477 |
| burkholderia humptydooensis sp. nov., a new species related to burkholderia thailandensis and the fifth member of the burkholderia pseudomallei complex. | during routine screening for burkholderia pseudomallei from water wells in northern australia in areas where it is endemic, gram-negative bacteria (strains msmb43(t), msmb121, and msmb122) with a similar morphology and biochemical pattern to b. pseudomallei and b. thailandensis were coisolated with b. pseudomallei on ashdown's selective agar. to determine the exact taxonomic position of these strains and to distinguish them from b. pseudomallei and b. thailandensis, they were subjected to a seri ... | 2017 | 27986727 |
| goodpasture disease as a consequence of melioidosis. | we describe a case of anti-glomerular basement membrane (gbm) antibody-mediated disease in association with concomitant burkholderia pseudomallei (melioidosis) bacteraemia. the temporal profile of the illness and initial absence of circulating anti-gbm antibodies, in light of the subsequent definitive histological diagnosis of anti-gbm disease, makes this case interesting and unusual. additionally, there have been no prior case reports suggesting melioidosis as a cause of biopsy-proven glomerulo ... | 2016 | 27981771 |
| melioidosis parotitis in children. | a recent paper published in jvatitd reporting a child in hainan with parotitis caused by burkholderia pseudomallei misleadingly described parotitis as a rare manifestation of melioidosis. in fact, it is one of the commonest forms of paediatric melioidosis seen in other parts of southeast asia, although interestingly not in australia. | 2016 | 27980534 |
| two-phase bactericidal mechanism of silver nanoparticles against burkholderia pseudomallei. | silver nanoparticles (agnps) have a strong antimicrobial activity against a variety of pathogenic bacteria. the killing mechanism of agnps involves direct physical membrane destruction and subsequent molecular damage from both agnps and released ag+. burkholderia pseudomallei is the causative agent of melioidosis, an endemic infectious disease primarily found in northern australia and southeast asia. b. pseudomallei is intrinsically resistant to most common antibiotics. in this study, the antimi ... | 2016 | 27977746 |
| utility of a lateral flow immunoassay (lfi) to detect burkholderia pseudomallei in soil samples. | culture is the gold standard for the detection of environmental b. pseudomallei. in general, soil specimens are cultured in enrichment broth for 2 days, and then the culture broth is streaked on an agar plate and incubated further for 7 days. however, identifying b. pseudomallei on the agar plates among other soil microbes requires expertise and experience. here, we evaluate a lateral flow immunoassay (lfi) developed to detect b. pseudomallei capsular polysaccharide (cps) in clinical samples as ... | 2016 | 27973567 |
| community-acquired pneumonia in the asia-pacific region. | community-acquired pneumonia (cap) is an important cause of mortality and morbidity worldwide. aging population, dense urbanization, and poor access to health care make the asia-pacific region vulnerable to cap. the high incidence of cap poses a significant health and economic burden in this region. common etiologic agents in other global regions including streptococcus pneumoniae, mycoplasma pneumoniae, haemophilus influenzae, chlamydophila pneumoniae, staphylococcus aureus, and respiratory vir ... | 2016 | 27960208 |
| community-acquired pneumonia: a global perspective. | community-acquired pneumonia (cap) is a global disease responsible for a large proportion of deaths and having significant economic cost. as diagnostic tools have increased in sensitivity, our understanding of the etiology of cap has begun to change with a significant increase in confirmed viral infections and the recognition that multiple pathogens are frequently present. empiric therapy remains the standard of care and guidelines are mostly based on published data from the united states or eur ... | 2016 | 27960204 |
| thermoregulation of biofilm formation in burkholderia pseudomallei is disrupted by mutation of a putative diguanylate cyclase. | burkholderia pseudomallei, a tier 1 select agent and the etiological agent of melioidosis, transitions from soil and aquatic environments to infect a variety of vertebrate and invertebrate hosts. during the transition from an environmental saprophyte to a mammalian pathogen, b. pseudomallei encounters and responds to rapidly changing environmental conditions. environmental sensing systems that control cellular levels of cyclic di-gmp promote pathogen survival in diverse environments. cyclic di-g ... | 2017 | 27956524 |
| in vivo distribution and clearance of purified capsular polysaccharide from burkholderia pseudomallei in a murine model. | burkholderia pseudomallei is the causative agent of melioidosis, a severe infection prominent in northern australia and southeast asia. the "gold standard" for melioidosis diagnosis is bacterial isolation, which takes several days to complete. the resulting delay in diagnosis leads to delayed treatments, which could result in death. in an attempt to develop better methods for early diagnosis of melioidosis, b. pseudomallei capsular polysaccharide (cps) was identified as an important diagnostic b ... | 2016 | 27941991 |
| promethazine improves antibiotic efficacy and disrupts biofilms of burkholderia pseudomallei. | efflux pumps are important defense mechanisms against antimicrobial drugs and maintenance of burkholderia pseudomallei biofilms. this study evaluated the effect of the efflux pump inhibitor promethazine on the structure and antimicrobial susceptibility of b. pseudomallei biofilms. susceptibility of planktonic cells and biofilms to promethazine alone and combined with antimicrobials was assessed by the broth microdilution test and biofilm metabolic activity was determined with resazurin. the effe ... | 2017 | 27936915 |
| identification of candidate host cell factors required for actin-based motility of burkholderia pseudomallei. | intracellular actin-based motility of the melioidosis pathogen burkholderia pseudomallei requires the bacterial factor bima. located at one pole of the bacterium, bima recruits and polymerizes cellular actin to promote bacterial motility within and between cells. here, we describe an affinity approach coupled with mass spectrometry to identify cellular proteins recruited to bima-expressing bacteria under conditions that promote actin polymerization. we identified a group of cellular proteins tha ... | 2016 | 27934296 |
| anion inhibition profiles of the γ-carbonic anhydrase from the pathogenic bacterium burkholderia pseudomallei responsible of melioidosis and highly drug resistant to common antibiotics. | burkholderia pseudomallei is a gram-negative saprophytic bacterium responsible of melioidosis, an endemic disease of tropical and sub-tropical regions of the world. a recombinant γ-ca (bpsγca) identified in the genome of this bacterium was cloned and purified. its catalytic activity and anion inhibition profiles were investigated. the enzyme was an efficient catalyst for the co2 hydration showing a kcat of 5.3×10(5)s(-1) and kcat/km of 2.5×10(7)m(-1)×s(-1). the best bpsγca inhibitors were sulfam ... | 2017 | 27914949 |
| environmental free-living amoebae isolated from soil in khon kaen, thailand, antagonize burkholderia pseudomallei. | presence of burkholderia pseudomallei in soil and water is correlated with endemicity of melioidosis in southeast asia and northern australia. several biological and physico-chemical factors have been shown to influence persistence of b. pseudomallei in the environment of endemic areas. this study was the first to evaluate the interaction of b. pseudomallei with soil amoebae isolated from b. pseudomallei-positive soil site in khon kaen, thailand. four species of amoebae, paravahlkampfia ustiana, ... | 2016 | 27898739 |
| burkholderia pseudomallei-derived mir-3473 enhances nf-κb via targeting traf3 and is associated with different inflammatory responses compared to burkholderia thailandensis in murine macrophages. | burkholderia pseudomallei (bp) is the causative agent of melioidosis, a kind of tropical disease. burkholderia thailandensis (bt), with a high sequence similarity to bp, is thought to be an avirulent organism. since there are numerous similarities between bp and bt, their differences in pathogenesis of host response and related mechanism are still undermined. in recent years, micrornas have been researched in many diseases, but seldom involved in bacterial infection, bacteria-host interaction or ... | 2016 | 27894256 |
| burkholderia pseudomallei γ-carbonic anhydrase is strongly activated by amino acids and amines. | activation of the γ-class carbonic anhydrase (cas, ec 4.2.1.1) from the pathogenic bacterium burkholderia pseudomallei (bpsγca) with a series of natural and non-natural amino acids and aromatic/heterocyclic amines has been investigated. the best bpsγca activators were d-his, l-dopa, d-trp, 4-amino-l-phe, dopamine, 2-(2-aminoethyl)pyridine, 2-aminoethyl-piparazine/morpholine and l-adrenaline, which showed activation constants ranging between 9 and 86nm. the least effective activators were l-his, ... | 2017 | 27881231 |
| a retrospective analysis of melioidosis in cambodian children, 2009-2013. | melioidiosis, infection by burkholderia pseudomallei, is an important but frequently under-recognised cause of morbidity and mortality in southeast asia and elsewhere in the tropics. data on the epidemiology of paediatric melioidosis in cambodia are extremely limited. | 2016 | 27871233 |
| a comparison of the immunological potency of burkholderia lipopolysaccharides in endotoxemic balb/c mice. | lipopolysaccharide is one of the virulence factors of the soil-borne pathogens burkholderia pseudomallei, b. thailandensis, b. cenocepacia and b. multivorans, which cause septic melioidosis (often in b. pseudomallei infections but rarely in b. thailandensis infections) or cepacia syndromes (commonly in b. cenocepacia infections but rarely in b. multivorans infections). the inflammatory responses in burkholderia lps-induced endotoxemia were evaluated in this study. prior to induction, the conserv ... | 2016 | 27862204 |
| pediatric suppurative parotitis caused by burkholderia pseudomallei. | suppurative parotitis caused by burkholderia pseudomallei has been rarely found outside endemic areas. | 2016 | 27843446 |
| chronic melioidosis presenting with multiple abscesses. | melioidosis is common in australia and southeast asia and is increasingly recognized in india. it presents in various forms which are difficult to identify and often mimics suppurative infections, tuberculosis, fungal infections, malignancy and systemic rheumatic diseases. presentation may vary from local disease to disseminated abscesses, pneumonia and sepsis. disease is common and severe in diabetics. we describe a case of diabetic man presenting with fever, septic shock, peri-articular nodule ... | 2016 | 27822380 |
| antimicrobial activity of tachyplesin 1 against burkholderia pseudomallei: an in vitro and in silico approach. | burkholderia pseudomallei, the causative agent of melioidosis, is intrinsically resistant to many conventional antibiotics. therefore, alternative antimicrobial agents such as antimicrobial peptides (amps) are extensively studied to combat this issue. our study aims to identify and understand the mode of action of the potential amp(s) that are effective against b. pseudomallei in both planktonic and biofilm state as well as to predict the possible binding targets on using in vitro and in silico ... | 2016 | 27812400 |
| septicemic melioidosis in a transplant recipient causing graft dysfunction. | melioidosis, an infectious disease caused by burkholderia pseudomallei, is endemic in the northern australia and south-east asia. it is an emerging disease in the indian subcontinent. melioidosis tends to run a potentially lethal course in immunocompromised individuals and data in renal transplantation are scarce. the clinical presentation of melioidosis is diverse, mimicking several other infectious diseases. diagnosis could be delayed in transplant recipients. choice and duration of antimicrob ... | 2016 | 27795637 |
| immune modulation as an effective adjunct post-exposure therapeutic for b. pseudomallei. | melioidosis is caused by the facultative intracellular bacterium burkholderia pseudomallei and is potentially fatal. despite a growing global burden and high fatality rate, little is known about the disease. recent studies demonstrate that cyclooxygenase-2 (cox-2) inhibition is an effective post-exposure therapeutic for pulmonary melioidosis, which works by inhibiting the production of prostaglandin e2 (pge2). this treatment, while effective, was conducted using an experimental cox-2 inhibitor t ... | 2016 | 27792775 |
| cutaneous melioidosis. | melioidosis is an emerging infection in india. it usually presents as pneumonia. melioidosis presenting as cutaneous lesions is uncommon. we present a case of cutaneous melioidosis from southern india. cutaneous melioidosis can present as an ulcer, pustule or as crusted erythematous lesions. a 22-year-old gentleman known case of diabetes mellitus was admitted in our hospital with an ulcer over the left thigh. discharge from the ulcer grew burkholderia pseudomallei. he was successfully treated wi ... | 2016 | 27790560 |
| a case-control study of community-acquired acinetobacter baumannii pneumonia and melioidosis pneumonia in northeast thailand: an emerging fatal disease with unique clinical features. | acinetobacter baumannii is the emerging cause of severe and often fatal gram-negative, community-acquired pneumonia (cap-ab) in thailand. due to its rarity, its specific clinical features are ill defined. in this retrospective study, we compared the demographic data, risk factors, clinical characteristics, radiographic pattern, and microbiological data between cap-ab and burkholderia pseudomallei cap (cap-bp) to identify the clinical features and risk factors of cap-ab. cap-ab was associated wit ... | 2017 | 27789057 |
| characterization of homologous sphingosine-1-phosphate lyase isoforms in the bacterial pathogen burkholderia pseudomallei. | sphingolipids (sls) are ubiquitous elements in eukaryotic membranes and are also found in some bacterial and viral species. as well as playing an integral structural role, sls also act as potent signaling molecules involved in numerous cellular pathways and have been linked to many human diseases. a central sl signaling molecule is sphingosine-1-phosphate (s1p), whose breakdown is catalyzed by s1p lyase (s1pl), a pyridoxal 5'-phosphate (plp)-dependent enzyme that catalyzes the cleavage of s1p to ... | 2016 | 27784725 |
| comparison of five commercial nucleic acid extraction kits for the pcr-based detection of burkholderia pseudomallei dna in formalin-fixed, paraffin-embedded tissues. | the extraction and further processing of nucleic acids (na) from formalin-fixed paraffin-embedded (ffpe) tissues for microbiological diagnostic polymerase chain reaction (pcr) approaches is challenging. here, we assessed the effects of five different commercially available nucleic acid extraction kits on the results of real-time pcr. ffpe samples from organs of burkholderia pseudomallei-infected swiss mice were subjected to processing with five different extraction kits from qiagen (ffpe dna tis ... | 2016 | 27766174 |
| trojan horse l-selectin monocytes: a portal of burkholderia pseudomallei entry into the brain. | 2016 | 27763813 | |
| characterization of in vitro phenotypes of burkholderia pseudomallei and burkholderia mallei strains potentially associated with persistent infection in mice. | burkholderia pseudomallei (bp) and burkholderia mallei (bm), the agents of melioidosis and glanders, respectively, are tier 1 biothreats. they infect humans and animals, causing disease ranging from acute and fatal to protracted and chronic. chronic infections are especially challenging to treat, and the identification of in vitro phenotypic markers which signal progression from acute to persistent infection would be extremely valuable. first, a phenotyping strategy was developed employing colon ... | 2017 | 27738703 |
| peritoneal dialysis-related peritonitis due to melioidosis: a potentially devastating condition. | ♦ background: melioidosis, an infectious disease caused by burkholderia pseudomallei, is endemic in southeast asia and northern australia. although a wide range of clinical manifestations from this organism are known, peritonitis associated with peritoneal dialysis (pd) has rarely been reported. ♦ patients and methods: peritoneal dialysis patients from all regions in thailand were eligible for the study if they had peritonitis and either peritoneal fluid or effluent culture positive for b. pseud ... | 2017 | 27738086 |
| a quadruplex real-time pcr assay for the rapid detection and differentiation of the most relevant members of the b. pseudomallei complex: b. mallei, b. pseudomallei, and b. thailandensis. | the burkholderia pseudomallei complex classically consisted of b. mallei, b. pseudomallei, and b. thailandensis, but has now expanded to include b. oklahomensis, b. humptydooensis, and three unassigned burkholderia clades. methods for detecting and differentiating the b. pseudomallei complex has been the topic of recent research due to phenotypic and genotypic similarities of these species. b. mallei and b. pseudomallei are recognized as cdc tier 1 select agents, and are the causative agents of ... | 2016 | 27736903 |
| melioidosis: series of eight cases. | melioidosis caused by the gram-negative bacterium burkholderia pseudomallei is a very serious infection and has been sporadically reported from the indian subcontinent. this disease entity can have acute and chronic presentations involving different organ systems. the purpose of this study is to analyze the risk factors, clinical presentations, therapy and outcome of culture proven cases of melioidosis. | 2016 | 27735148 |
| enhanced surveillance for fatal dengue-like acute febrile illness in puerto rico, 2010-2012. | dengue is a leading cause of morbidity throughout the tropics; however, accurate population-based estimates of mortality rates are not available. | 2016 | 27727271 |
| a fatal case of melioidosis with pancytopenia in a traveler from indonesia. | melioidosis, an infectious disease with high mortality, caused by burkholderia pseudomallei, is endemic in southeast asia and northern australia. in indonesia, autochthonous cases have been rarely reported, with most cases being sporadic and occurring in travelers. herein, we report a fatal case of neurological melioidosis in a traveler from indonesia presenting with septic shock. | 2017 | 27720346 |
| glibenclamide impairs responses of neutrophils against burkholderia pseudomallei by reduction of intracellular glutathione. | the major risk factor for melioidosis, an infectious disease caused by b. pseudomallei, is diabetes mellitus. more than half of diabetic melioidosis patients in thailand were prescribed glibenclamide. recent evidence demonstrates that glibenclamide reduces pro-inflammatory cytokine production by polymorphonuclear neutrophils (pmns) of diabetic individuals in response to this bacterial infection. however, the mechanisms by which glibenclamide affects cytokine production are unknown. we found that ... | 2016 | 27713554 |
| burkholderia pseudomallei resistance to antibiotics in biofilm-induced conditions is related to efflux pumps. | burkholderia pseudomallei, the causative agent of melioidosis, has been found to increase its resistance to antibiotics when growing as a biofilm. the resistance is related to several mechanisms. one of the possible mechanisms is the efflux pump. using bioinformatics analysis, it was found that bpsl1661, bpsl1664 and bpsl1665 were orthologous genes of the efflux transporter encoding genes for biofilm-related antibiotic resistance, pa1874-pa1877 genes in pseudomonas aeruginosa strain pao1. expres ... | 2016 | 27702426 |
| bee venom (apis mellifera) an effective potential alternative to gentamicin for specific bacteria strains: bee venom an effective potential for bacteria. | mellitine, a major component of bee venom (bv, apis mellifera), is more active against gram positive than gram negative bacteria. moreover, bv has been reported to have multiple effects, including antibacterial, antivirus, and anti-inflammation effects, in various types of cells. in addition, wasp venom has been reported to have antibacterial properties. the aim of this study was to evaluate the antibacterial activity of bv against selected gram positive and gram negative bacterial strains of me ... | 2016 | 27695631 |
| soil nutrient depletion is associated with the presence of burkholderia pseudomallei. | burkholderia pseudomallei is a soil-dwelling bacterium and the cause of melioidosis, which kills an estimated 89,000 people per year worldwide. agricultural workers are at high risk of infection due to repeated exposure to the bacterium. little is known about the soil physicochemical properties associated with the presence or absence of the organism. here, we evaluated the soil physicochemical properties and presence of b. pseudomallei in 6,100 soil samples collected from 61 rice fields in thail ... | 2016 | 27694236 |
| susceptibility of select agents to predation by predatory bacteria. | select agents are microorganisms and toxins considered to be exploitable as biological weapons. although infections by many select agents can be treated by conventional antibiotics, the risk of an emerging or engineered drug resistant strain is of great concern. one group of microorganisms that is showing potential to control drug resistant gram-negative bacteria are the predatory bacteria from the genera bdellovibrio spp. and micavibrio spp. in this study, we have examined the ability of bdello ... | 2015 | 27682124 |
| nematode peptides with host-directed anti-inflammatory activity rescue caenorhabditis elegans from a burkholderia pseudomallei infection. | burkholderia pseudomallei, the causative agent of melioidosis, is among a growing number of bacterial pathogens that are increasingly antibiotic resistant. antimicrobial peptides (amps) have been investigated as an alternative approach to treat microbial infections, as generally, there is a lower likelihood that a pathogen will develop resistance to amps. in this study, 36 candidate caenorhabditis elegans genes that encode secreted peptides of <150 amino acids and previously shown to be overexpr ... | 2016 | 27672387 |
| analyses of the distribution patterns of burkholderia pseudomallei and associated phages in soil samples in thailand suggest that phage presence reduces the frequency of bacterial isolation. | burkholderia pseudomallei is a soil saprophytic bacterium that causes melioidosis. the infection occurs through cutaneous inoculation, inhalation or ingestion. bacteriophages (phages) in the same ecosystem may significantly impact the biology of this bacterium in the environment, and in their culturability in the laboratory. | 2016 | 27668750 |
| burkholderia pseudomallei: challenges for the clinical microbiology laboratory. | melioidosis is a potentially fatal infection caused by the bacterium burkholderia pseudomallei clinical diagnosis of melioidosis can be challenging since there is no pathognomonic clinical syndrome, and the organism is often misidentified by methods used routinely in clinical laboratories. although the disease is more prevalent in thailand and northern australia, sporadic cases may be encountered in areas where it is not endemic, including the united states. since the organism is considered a ti ... | 2016 | 27654336 |
| dbsecsys 2.0: a database of burkholderia mallei and burkholderia pseudomallei secretion systems. | burkholderia mallei and b. pseudomallei are the causative agents of glanders and melioidosis, respectively, diseases with high morbidity and mortality rates. b. mallei and b. pseudomallei are closely related genetically; b. mallei evolved from an ancestral strain of b. pseudomallei by genome reduction and adaptation to an obligate intracellular lifestyle. although these two bacteria cause different diseases, they share multiple virulence factors, including bacterial secretion systems, which repr ... | 2016 | 27650316 |
| involvement of l-selectin expression in burkholderia pseudomallei-infected monocytes invading the brain during murine melioidosis. | the development of neurologic melioidosis was linked to the elicitation of burkholderia pseudomallei-infected l-selectin(hi)cd11b(+) balb/c cells in our previous study. however, whether monocytic l-selectin (cd62l, encoded by the sell gene) is a key factor remains uncertain. in the present study, after establishing multi-organ foci via hematogenous routes, we demonstrated that b. pseudomallei gfp steadily persisted in blood, splenic, hepatic and bone marrow (bm) ly6c monocytes; however, the circ ... | 2016 | 27646437 |
| formulation and characterization of nanocluster ceftazidime for the treatment of acute pulmonary melioidosis. | melioidosis is an infectious disease caused by burkholderia pseudomallei. the disease is responsible for a high proportion of human pneumonia and fatal bacteremia in the endemic areas of the world and is highly resistant to most commonly available antibiotics. studies have shown that prophylactic antibiotic treatment, when administered 24 h following bacterial challenge, can prevent infection in a murine model. prophylactic treatment against this disease using a pulmonary antibiotic formulation ... | 2016 | 27639659 |
| cloning, expression, and characterization of a peptidoglycan hydrolase from the burkholderia pseudomallei phage st79. | the lytic phage st79 of burkholderia pseudomallei can lyse a broad range of its host including antibiotic resistant isolates from within using a set of proteins, holin, lysb, lysc and endolysin, a peptidoglycan (pg) hydrolase enzyme. the phage st79 endolysin gene identified as peptidase m15a was cloned, expressed and purified to evaluate its potential to lyse pathogenic bacteria. the molecular size of the purified enzyme is approximately 18 kda and the in silico study cited here indicated the pr ... | 2016 | 27637947 |
| physicochemical properties influencing presence of burkholderia pseudomallei in soil from small ruminant farms in peninsular malaysia. | soil is considered to be a major reservoir of burkholderia pseudomallei in the environment. this paper investigates soil physicochemical properties that may influence presence of b. pseudomallei in soil samples from small ruminant farms in peninsular malaysia. soil samples were collected from the farms and cultured for b. pseudomallei. the texture, organic matter and water contents, ph, elemental contents, cation exchange capacities, carbon, sulfur and nitrogen contents were determined. analysis ... | 2016 | 27635652 |
| eukaryotic pathways targeted by the type iii secretion system effector protein, bipc, involved in the intracellular lifecycle of burkholderia pseudomallei. | burkholderia pseudomallei, the etiological agent for melioidosis, is known to secrete a type iii secretion system (ttss) protein into the host's internal milieu. one of the ttss effector protein, bipc, has been shown to play an important role in the b. pseudomallei pathogenesis. to identify the host response profile that was directly or indirectly regulated by this protein, genome-wide transcriptome approach was used to examine the gene expression profiles of infected mice. the transcriptome ana ... | 2016 | 27634329 |
| characterization of secreted sphingosine-1-phosphate lyases required for virulence and intracellular survival of burkholderia pseudomallei. | sphingosine-1-phosphate (s1p), a bioactive sphingolipid metabolite, plays a critical role in the orchestration of immune responses. s1p levels within the mammalian host are tightly regulated, in part through the activity of s1p lyase (s1pl) which catalyses its irreversible degradation. herein, we describe the identification and characterization of secreted s1pl orthologues encoded by the facultative intracellular bacteria burkholderia pseudomallei and burkholderia thailandensis. these bacterial ... | 2016 | 27632710 |
| prevalence and identification of burkholderia pseudomallei and near-neighbor species in the malabar coastal region of india. | accurate identification of pathogens with biowarfare importance requires detection tools that specifically differentiate them from near-neighbor species. burkholderia pseudomallei, the causative agent of a fatal disease melioidosis, is one such biothreat agent whose differentiation from its near-neighbor species is always a challenge. this is because of its phenotypic similarity with other burkholderia species which have a wide spread geographical distribution with shared environmental niches. m ... | 2016 | 27632353 |
| a case on acute burkholderia pseudomallei pericarditis. | 2016 | 27625102 | |
| flexible vs rigid epitope conformations for diagnostic- and vaccine-oriented applications: novel insights from the burkholderia pseudomallei bpsl2765 pal3 epitope. | peptides seldom retain stable conformations if separated from their native protein structure. in an immunological context, this potentially affects the development of selective peptide-based bioprobes and, from a vaccine perspective, poses inherent limits in the elicitation of cross-reactive antibodies by candidate epitopes. here, a 1,4-disubstituted-1,2,3-triazole-mediated stapling strategy was used to stabilize the native α-helical fold of the pal3 peptidic epitope from the protein antigen pal ... | 2016 | 27623032 |
| antibiotic resistance in burkholderia species. | the genus burkholderia comprises metabolically diverse and adaptable gram-negative bacteria, which thrive in often adversarial environments. a few members of the genus are prominent opportunistic pathogens. these include burkholderia mallei and burkholderia pseudomallei of the b. pseudomallei complex, which cause glanders and melioidosis, respectively. burkholderia cenocepacia, burkholderia multivorans, and burkholderia vietnamiensis belong to the burkholderia cepacia complex and affect mostly c ... | 2016 | 27620956 |
| development, synthesis and structure-activity-relationships of inhibitors of the macrophage infectivity potentiator (mip) proteins of legionella pneumophila and burkholderia pseudomallei. | the bacteria burkholderia pseudomallei and legionella pneumophila cause severe diseases like melioidosis and legionnaire's disease with high mortality rates despite antibiotic treatment. due to increasing antibiotic resistances against these and other gram-negative bacteria, alternative therapeutical strategies are in urgent demand. as a virulence factor, the macrophage infectivity potentiator (mip) protein constitutes an attractive target. the mip proteins of b. pseudomallei and l. pneumophila ... | 2016 | 27591009 |
| a burkholderia pseudomallei infection imported from eritrea to israel. | although it has been predicted that melioidosis is probably endemic in the horn of africa, no confirmed cases have ever been detected in the region. we have recently isolated burkholderia pseudomallei from an eritrean patient in israel. the isolate was assigned a novel multilocus sequence type (st-1479). the observation has important epidemiological implications in an era of massive human migration. | 2016 | 27573625 |
| predicted global distribution of burkholderia pseudomallei and burden of melioidosis. | burkholderia pseudomallei, a highly pathogenic bacterium that causes melioidosis, is commonly found in soil in southeast asia and northern australia(1,2). melioidosis can be difficult to diagnose due to its diverse clinical manifestations and the inadequacy of conventional bacterial identification methods(3). the bacterium is intrinsically resistant to a wide range of antimicrobials, and treatment with ineffective antimicrobials may result in case fatality rates (cfrs) exceeding 70%(4,5). the im ... | 2016 | 27571754 |
| differential production of type i ifn determines the reciprocal levels of il-10 and proinflammatory cytokines produced by c57bl/6 and balb/c macrophages. | pattern recognition receptors detect microbial products and induce cytokines, which shape the immunological response. il-12, tnf-α, and il-1β are proinflammatory cytokines, which are essential for resistance against infection, but when produced at high levels they may contribute to immunopathology. in contrast, il-10 is an immunosuppressive cytokine, which dampens proinflammatory responses, but it can also lead to defective pathogen clearance. the regulation of these cytokines is therefore centr ... | 2016 | 27549173 |
| trehalose metabolism: a sweet spot for burkholderia pseudomallei virulence. | 2017 | 27540767 | |
| melioidosis in travelers returning from vietnam to france. | 2016 | 27532771 | |
| burkholderia pseudomallei biofilm promotes adhesion, internalization and stimulates proinflammatory cytokines in human epithelial a549 cells. | burkholderia pseudomallei is a gram-negative bacterium that causes melioidosis. inhalational exposure leading to pulmonary melioidosis is the most common clinical manifestation with significant mortality. however, the role of b. pseudomallei biofilm phenotype during bacterial-host interaction remains unclear. we hypothesize that biofilm phenotype may play a role in such interactions. in this study, b. pseudomallei h777 (biofilm wild type), b. pseudomallei m10 (biofilm mutant) and b. pseudomallei ... | 2016 | 27529172 |
| melioidosis: a rare cause of liver abscess. | case presentation. this is a case of a 44-year-old male, farmer, known to be diabetic, presenting with two-week history of vague abdominal pain associated with high grade fever. abdominal ct scan showed localized liver abscess at segment 8 measuring 7.5 × 6.8 × 6.1 cm. patient subsequently underwent laparoscopic ultrasound guided pigtail insertion for drainage of abscess. culture studies showed moderate growth of burkholderia pseudomallei in which the patient completed seven days of iv meropenem ... | 2016 | 27529039 |
| burkholderia thailandensis: growth and laboratory maintenance. | burkholderia thailandensis is a nonpathogenic gram-negative bacterium found in tropical soils. closely related to several human pathogens, its ease of genetic manipulation, rapid growth in the laboratory, and low virulence make b. thailandensis a commonly used model organism. this unit describes the fundamental protocols for in vitro growth and maintenance of b. thailandensis in the laboratory. © 2016 by john wiley & sons, inc. | 2016 | 27517336 |
| the antibiotics of choice for the treatment of melioidosis in indian set up. | therapeutic options for the treatment of melioidosis caused by burkholderia pseudomallei are limited due to the inherent resistance conferred by this pathogen to various groups of antibiotics. witnessing an increase in the number of microbiological culture-confirmed cases of melioidosis at our settings in the past few years, we undertook this study to estimate the minimum inhibitory concentrations of clinical isolates of b. pseudomallei against the four commonly employed antimicrobial agents in ... | 2017 | 27514960 |
| neurological melioidosis presenting as intracranial abscess. | melioidosis is an emerging disease in our country caused by the bacteria burkholderia pseudomallei. melioidosis can virtually affect any organ. it has varying clinical presentations ranging from pneumonia to fatal sepsis. central nervous system (cns) involvement in melioidosis is rare. we present a case of cns melioidosis from south india. as the incidence of diabetes is increasing in our country varying manifestations of melioidosis will be seen in routine clinical practice. melioidosis should ... | 2017 | 27510695 |
| melioidosis in a patient with chronic rhinosinusitis. | melioidosis is a serious infection caused by soil-dwelling gram-negative bacillus burkholderia pseudomallei. it is most commonly reported in northern australia, southeast and southern asia, china, and taiwan. | 2016 | 27488340 |
| mapping epigenetic changes to the host cell genome induced by burkholderia pseudomallei reveals pathogen-specific and pathogen-generic signatures of infection. | the potential for epigenetic changes in host cells following microbial infection has been widely suggested, but few examples have been reported. we assessed genome-wide patterns of dna methylation in human macrophage-like u937 cells following infection with burkholderia pseudomallei, an intracellular bacterial pathogen and the causative agent of human melioidosis. our analyses revealed significant changes in host cell dna methylation, at multiple cpg sites in the host cell genome, following infe ... | 2016 | 27484700 |
| biocidal and sporicidal efficacy of pathoster(®) 0.35% and pathoster(®) 0.50% against bacterial agents in potential bioterrorism use. | the use of products that can neutralize or significantly reduce the microbial load and that are not harmful to human health and the environment represents a milestone in the fight against the spread of infectious diseases. peracetic acid, besides being an excellent sterilizing and sporicidal agent, is harmless to humans and the environment when it is used in a common dosage. however, the high costs and loss of efficacy of the product very quickly after its reconstitution limit its use. we evalua ... | 2016 | 27482880 |
| barriers and recommended interventions to prevent melioidosis in northeast thailand: a focus group study using the behaviour change wheel. | melioidosis, an often fatal infectious disease in northeast thailand, is caused by skin inoculation, inhalation or ingestion of the environmental bacterium, burkholderia pseudomallei. the major underlying risk factor for melioidosis is diabetes mellitus. recommendations for melioidosis prevention include using protective gear such as rubber boots and gloves when in direct contact with soil and environmental water, and consuming bottled or boiled water. only a small proportion of people follow su ... | 2016 | 27472421 |
| role of canonical and non-canonical inflammasomes during burkholderia infection. | burkholderia pseudomallei is a gram-negative flagellate bacterium that causes melioidosis, a disease endemic to southeast asia and other tropical regions. following infection of macrophages and other non-phagocytic cell types, b. pseudomallei or b. thailandensis (a related species that causes disease in mice but not humans) are able to escape the phagosome and replicate in the host cell cytoplasm. resistance to infection with burkholderia is dependent on the nlrp3 and nlrc4 inflammasomes and the ... | 2016 | 27460811 |
| functional diversity of cytotoxic trnase/immunity protein complexes from burkholderia pseudomallei. | contact-dependent growth inhibition (cdi) is a widespread mechanism of inter-bacterial competition. cdi(+) bacteria deploy large cdia effector proteins, which carry variable c-terminal toxin domains (cdia-ct). cdi(+) cells also produce cdii immunity proteins that specifically neutralize cognate cdia-ct toxins to prevent auto-inhibition. here, we present the crystal structure of the cdia-ct/cdii(e479) toxin/immunity protein complex from burkholderia pseudomallei isolate e479. the cdia-ct(e479) tr ... | 2016 | 27445337 |
| the art of persistence-the secrets to burkholderia chronic infections. | the gram-negative proteobacteria genus burkholderia encompasses multiple bacterial species that are pathogenic to humans and other vertebrates. two pathogenic species of interest within this genus are burkholderia pseudomallei (bpm) and the b. cepacia complex (bcc); the former is the causative agent of melioidosis in humans and other mammals, and the latter is associated with pneumonia in immunocompromised patients. one understudied and shared characteristic of these two pathogenic groups is the ... | 2016 | 27440810 |
| cutaneous melioidosis cluster caused by contaminated wound irrigation fluid. | 2016 | 27438887 | |
| melioidosis: a greater threat than previously suspected? | research released in 2016 shows that the global burden of burkholderia pseudomallei infection is probably much higher than previously suspected. better testing and reporting are needed if we are to detect outbreaks and diminish deadly delays in treatment. worldwide, researchers need support for vaccine development, particularly for public health vaccines to protect people and animals in endemic areas. because melioidosis can infect so many species, collaboration and communication between human a ... | 2016 | 27436814 |
| multilocus sequence typing of clinical isolates of burkholderia pseudomallei collected in hainan, a tropical island of southern china. | burkholderia pseudomallei is a gram-negative bacterium that causes melioidosis. in this study, we examined the sequence types (sts) of 60 clinical isolates from patients with melioidosis in hainan, a tropical island in southern china. the 60 clinical isolates were resolved into 30 sts. among the sts, st562 was also reported in australia, and st90 was also reported in the united states, probably from a traveler who had previously visited asia. in addition, six novel sts were found in this study, ... | 2016 | 27430537 |
| rapid and sensitive multiplex detection of burkholderia pseudomallei-specific antibodies in melioidosis patients based on a protein microarray approach. | the environmental bacterium burkholderia pseudomallei causes the infectious disease melioidosis with a high case-fatality rate in tropical and subtropical regions. direct pathogen detection can be difficult, and therefore an indirect serological test which might aid early diagnosis is desirable. however, current tests for antibodies against b. pseudomallei, including the reference indirect haemagglutination assay (iha), lack sensitivity, specificity and standardization. consequently, serological ... | 2016 | 27427979 |
| improved multilocus sequence typing of burkholderia pseudomallei and closely related species. | the burkholderiapseudomallei multilocus sequence typing (mlst) database (http://pubmlst.org/bpseudomallei/) contains the largest global sequence repository for b. pseudomallei and its closest genetic relatives. using conventional mlst and in silico mlst data derived from publicly available whole-genome sequences, we first defined the phylogenetic relatedness of b. pseudomallei and its nearest neighbours. based on this analysis, we propose that the recently described b. pseudomallei complex (bpc) ... | 2016 | 27412128 |
| experimental phage therapy for burkholderia pseudomallei infection. | burkholderia pseudomallei is an intracellular gram-negative bacterial pathogen intrinsically resistant to a variety of antibiotics. phages have been developed for use as an alternative treatment therapy, particularly for bacterial infections that do not respond to conventional antibiotics. in this study, we investigated the use of phages to treat cells infected with b. pseudomallei. phage c34 isolated from seawater was purified and characterised on the basis of its host range and morphology usin ... | 2016 | 27387381 |
| burkholderia pseudomallei rapidly infects the brain stem and spinal cord via the trigeminal nerve after intranasal inoculation. | infection with burkholderia pseudomallei causes melioidosis, a disease with a high mortality rate (20% in australia and 40% in southeast asia). neurological melioidosis is particularly prevalent in northern australian patients and involves brain stem infection, which can progress to the spinal cord; however, the route by which the bacteria invade the central nervous system (cns) is unknown. we have previously demonstrated that b. pseudomallei can infect the olfactory and trigeminal nerves within ... | 2016 | 27382023 |
| choline catabolism in burkholderia thailandensis is regulated by multiple glutamine amidotransferase 1-containing arac family transcriptional regulators. | burkholderia thailandensis is a soil-dwelling bacterium that shares many metabolic pathways with the ecologically similar, but evolutionarily distant, pseudomonas aeruginosa among the diverse nutrients it can utilize is choline, metabolizable to the osmoprotectant glycine betaine and subsequently catabolized as a source of carbon and nitrogen, similar to p. aeruginosa orthologs of genes in the choline catabolic pathway in these two bacteria showed distinct differences in gene arrangement as well ... | 2016 | 27381916 |
| brainstem micro-abscesses caused by burkholderia pseudomallei in a 10-month-old infant: a case report. | melioidosis is an uncommon tropical infectious disease caused by burkholderia pseudomallei. neurological complications of melioidosis are extremely uncommon in infants. a 10-month-old girl is described who presented with disseminated melioidosis with subcutaneous nodules, arthritis, hepatomegaly and a lung cavity, and developed a left medial rectus palsy. cranial mri demonstrated mid-brain, pontine and basal ganglia micro-abscesses. therapy with meropenem and cotrimoxazole led to resolution of t ... | 2016 | 27376438 |
| burkholderia pseudomallei differentially regulates host innate immune response genes for intracellular survival in lung epithelial cells. | burkholderia pseudomallei, the causative agent of melioidosis poses a serious threat to humankind. b. pseudomallei secretes numerous virulence proteins that alter host cell functions to escape from intracellular immune sensors. however, the events underlying disease pathogenesis are poorly understood. | 2016 | 27367858 |
| trehalase plays a role in macrophage colonization and virulence of burkholderia pseudomallei in insect and mammalian hosts. | trehalose is a disaccharide formed from two glucose molecules. this sugar molecule can be isolated from a range of organisms including bacteria, fungi, plants and invertebrates. trehalose has a variety of functions including a role as an energy storage molecule, a structural component of glycolipids and plays a role in the virulence of some microorganisms. there are many metabolic pathways that control the biosynthesis and degradation of trehalose in different organisms. the enzyme trehalase for ... | 2017 | 27367830 |
| genome sequence of the historical clinical isolate burkholderia pseudomallei phls 6. | here, we present the draft genome sequence of burkholderia pseudomallei phls 6, a virulent clinical strain isolated from a melioidosis patient in bangladesh in 1960. the draft genome consists of 39 contigs and is 7,322,181 bp long. | 2016 | 27365360 |
| the identification and differentiation between burkholderia mallei and burkholderia pseudomallei using one gene pyrosequencing. | the etiologic agents for melioidosis and glanders, burkholderia mallei and burkholderia pseudomallei respectively, are genetically similar making identification and differentiation from other burkholderia species and each other challenging. we used pyrosequencing to determine the presence or absence of an insertion sequence is407a within the flagellin p (flip) gene and to exploit the difference in orientation of this gene in the two species. oligonucleotide primers were designed to selectively t ... | 2014 | 27350960 |
| developing peptide mimotopes of capsular polysaccharides and lipopolysaccharides protective antigens of pathogenic burkholderia bacteria. | burkholderia pseudomallei (bp) and burkholderia mallei (bm) are two species of pathogenic burkholderia bacteria. our laboratory previously identified four monoclonal antibodies (mabs) that reacted against burkholderia capsular polysaccharides (ps) and lipopolysaccharides (lps) and effectively protected against a lethal dose of bp/bm infections in mice. in this study, we used phage display panning against three different phage peptide libraries to select phage clones specifically recognized by ea ... | 2016 | 27328059 |
| burkholderia pseudomallei rpos mediates inos suppression in human hepatocyte (hc04) cells. | burkholderia pseudomallei is an intracellular gram-negative bacterial pathogen and the causative agent of melioidosis, a widespread disease in southeast asia. reactive nitrogen, in an intermediate form of nitric oxide (no), is one of the first lines of defense used by host cells to eliminate intracellular pathogens, through the stimulation of inducible nitric oxide synthase (inos). studies in phagocytotic cells have shown that the inos response is muted in b. pseudomallei infection, and implicat ... | 2016 | 27324398 |