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an avidin-biotin enzyme-linked immunosorbent assay for the detection of pseudomonas pseudomallei antigens. 19902260180
[infection with pseudomonas pseudomallei].melioidosis, a severe, often fatal disease caused by infection with pseudomonas pseudomallei, has been thought to be a rare endemic disease relatively limited to the areas 20 degrees on either side of the equator. however, an increasing number of people travelling to these areas are reportedly suffering from this disease. it is timely to review this disease for doctors who are unfamiliar with this disease. p. pseudomallei, first discovered by whitmore and krishnaswami in 1912, is a gram-negative ...19902273564
in vitro susceptibilities of pseudomonas pseudomallei to 27 antimicrobial agents.clinical isolates of pseudomonas pseudomallei isolated in thailand from 1981 to 1989 were tested for their in vitro susceptibilities to 27 antimicrobial agents, including older and newer quinolones, broad-spectrum cephems, carbapenems, monobactams, penicillins, tetracyclines, chloramphenicol, rifamycin, sulfamethoxazole, trimethoprim, and fosfomycin. tosufloxacin, meropenem, cs-533, and minocycline were active against p. pseudomallei at levels comparable to or even greater than those of antimicr ...19902291671
survival of pseudomonas pseudomallei in human phagocytes.pseudomonas pseudomallei causes the disease melioidosis, with protean manifestations, protracted clinical course and unpredictable response to antimicrobial treatment. intracellular location of the organism is suspected to be the cause of these properties. this study was undertaken to examine the intracellular growth of this bacterium. intracellular growth and survival was assessed at different time intervals, by gram's stain and electronmicroscopic examination. during the first 5 h, the numbers ...19902304065
[the life strategy and self-regulation mechanisms of populations of the causative agents of sapronoses (exemplified by pseudomonas pseudomallei)].the strategy of the selection (life) of p. pseudomallei has been defined as c-competitiveness, combining the advantages of the limited (r and k) types of the ecological strategies of microorganisms and ensuring their good capacity of survival in soil biota. the self-regulation mechanisms of p. pseudomallei populations in the environment are determined by the type of their strategy of selection, which also determines the place of this species among other organisms inhabiting the soil. c-competiti ...19902330774
experimental infection of normal and immunosuppressed pigs with pseudomonas pseudomallei.a single dose of 5 x 10(8) bacilli of pseudomonas pseudomallei by intratracheal injection resulted in acute (21 cases) or chronic (19 cases) melioidosis in 40 of 48 pigs. fifteen (10 acute and 5 chronic) had been immunosuppressed by cyclophosphamide before inoculation. the major clinical signs were initial fever, marked neutrophilia and, in the acute cases, respiratory distress. there were no signs of the nasal and ocular discharge, paresis or diarrhoea seen in acute cases in south-east asia. th ...19902344335
in vitro activities of antimicrobial combination against pseudomonas pseudomallei.p pseudomallei infection was treated empirically with an antimicrobial combination without hard evidence of a more favorable outcome over single drug regimens. the so-called "conventional' agents, namely kanamycin (k), chloramphenicol (c), doxycycline (d) and sulfamethoxazole/trimethoprim (smz/tmp) are often combined. we determined the effects of the combination of these agents by standard time-kill curve. six combinations were tested, i.e. k and c, k and d, k and smz/tmp, c and d, c and smz/tmp ...19902351900
[the ultrastructural characteristics of the causative agent of melioidosis and its interaction with phagocytes in vivo].the electron microscopic studies have established that the virulent strain of pseudomonas pseudomallei c-141, an agent of melioidosis, being intraperitoneally administered to guinea pigs, forms in vivo three morphological variants. one variant is capsule-free, while two others have a capsule which in the second variant may be referred by its characters to the microcapsule and in the third one to the macrocapsule. it has been shown that under the interaction of these three morphological variants ...19902377083
[antigenic composition and the serological diagnosis of pseudomonas pseudomallei]. 19852412369
[the polysaccharide component of pseudomonas pseudomallei slime].the polysaccharide component contained in the slime of the causative agent of melioidosis was obtained. this component was found to be a biopolymer, mainly of the carbohydrate nature, consisting of galactose, glucose, mannose, rhamnose and two unidentified carbohydrates. the slime polysaccharide component contained two thermostable and acid-resistant antigens. the action of alkali led to the loss of one of these antigens. rabbit antisera to the preparations of the slime polysaccharide component ...19852418606
[immunomodulating activity of rna mononucleotides].the immunological action of rna mononucleotides was studied in animal experiments. the most pronounced activation of macrophagal glycolysis urea cycle, oxidative phosphorylation, lysosomal hydrolases was induced by uridine 5'-monophosphate (5'-ump) and guanosine 5'-monophosphate (5'-gmp); 5'-gmp also induced the maximum increase of the expression of fc gamma receptors. 5'-ump ensured cell activation comparable with the total action of all mononucleotides. 5'-ump and 5'-gmp, used in combination, ...19882452537
identification of pseudomonas pseudomallei in clinical practice: use of simple screening tests and api 20ne.the api 20ne kit and a simple screening system involving gram's stain, the oxidase reaction, colistin and gentamicin resistance, and colonial characteristics on a differential agar medium, were used to test 400 strains of pseudomonas pseudomallei. the api kit identified 390 (97.5%) strains correctly on first testing and all but one of the remainder on second testing. only one strain was initially misidentified (as ps cepacia). the screening system was 100% accurate in identifying ps pseudomallei ...19892472432
[a method of fixation and disinfection of microbial cells on magnetic sorbents].the 96% ethanol has been found fairly effective for the fixation and disinfection of yersinia pestis, vibrio cholerae, pseudomonas mallei, and pseudomonas pseudomallei after their contact with magnetic polyacrylamide sorbents containing specific immunoglobulins for ligands. such step is recommended for the quantitative immunofluorescence test employed for rapid analysis, diagnosis, and identification of the above microorganisms.19892474712
tropical disease in the immunocompromised host: melioidosis and pythiosis.melioidosis is an infection of humans and animals caused by a gram-negative motile bacillus, pseudomonas pseudomallei. forty-nine patients with melioidosis complicating diabetes mellitus, collagen vascular disorders, leukemia/lymphoma, and other hematologic malignancies are described. twenty-nine of these patients had disseminated/septicemic infection, two developed toxic shock syndrome, and one with aids experienced recrudescent melioidosis. patients with disseminated melioidosis often have a v ...19892602781
pseudomonas pseudomallei pneumonia with septicemia--case report.a case of pseudomonas pseudomallei pneumonia with septicemia is described. the onset was insidious with paucity of systemic symptoms except fever. diabetes mellitus and alcoholism were associated problems. initially blood cultures were negative but subsequently p. pseudomallei was isolated. the outcome was fatal. unless diagnosed early and treated appropriately, patients often succumb to septicemic shock.19892609180
inhibition of macromolecular synthesis in cultured macrophages by pseudomonas pseudomallei exotoxin.pseudomonas pseudomallei exotoxin was found to be a potent inhibitor of protein and dna synthesis in cultured macrophages. inhibition of dna synthesis occurred at toxin concentrations as low as 1-2 micrograms/ml and inhibition of 3h-thymidine uptake was almost complete at concentrations of 8 micrograms/ml or more. a close correlation between cell damage and inhibition by dna synthesis was observed. for protein synthesis, inhibition was obtained at much lower doses (0.06-2.0 micrograms/ml) of the ...19892615673
the activity of amoxycillin/clavulanic acid against pseudomonas pseudomallei. 19892621173
high activity of acid phosphatase of pseudomonas pseudomallei as a possible attribute relating to its pathogenicity.phosphatase activities were compared quantitatively among selected species of pseudomonads. p. pseudomallei showed the highest activity of a bell-shaped ph pattern with a peak at around ph 5.0. p. cepacia had a similar pattern of milder intensity. in contrast, p. aeruginosa revealed an alkaline phosphatase activity with a ph optimum higher than 8.0, but the level of activity was much lower than those of the above two species. the enzymatic reactions of other species were slight or negligible at ...19892632842
melioidosis with multiple cerebral abscesses.melioidosis from pseudomonas pseudomallei is common in endemic areas (particularly southeast asia) and is being recognized with increasing frequency in developed countries. central nervous system involvement is a rare complication with a high mortality. a patient with multiple cerebral abscesses caused by this organism is presented to demonstrate that successful treatment is possible when a high index of clinical suspicion leads to early diagnosis.19892653340
melioidosis: review and update.melioidosis prevails in southeast asia and northern australia. sporadic cases have been increasingly reported from countries located between 20 degrees north latitude and 20 degrees south latitude as well as in travelers and in soldiers who have resided in these areas. the organisms are commonly found in water and soil and are usually transmitted to humans by cutaneous or respiratory routes. clinical manifestations range from subclinical infection to overwhelming septicemia that resembles dissem ...19892665001
laboratory survey of fluoroquinolone activity.fluoroquinolones are active against a wide variety of bacteria. the antibacterial spectra of fluoroquinolones encompass staphylococci, bacillus species, and corynebacterium species implicated in infections of the immunocompromised host; enterobacteriaceae; most intestinal pathogens; and many gram-negative organisms commonly causing nosocomial infections. haemophilus influenzae, haemophilus ducreyi, neisseria gonorrhoeae, neisseria meningitidis, and branhamella catarrhalis are highly susceptible ...19892672262
the antimicrobial susceptibility of pseudomonas pseudomallei. emergence of resistance in vitro and during treatment.we have measured the in-vitro activity of 27 antimicrobials against 211 clinical and ten reference strains of pseudomonas pseudomallei. imipenem was the most active antibiotic tested, followed by piperacillin, doxycycline, amoxycillin/clavulanic acid, cefixime, cefetamet, azlocillin and ceftazidime, all of which had mics of less than or equal to 2 mg/l for the majority of strains. the measured mics were dependent on the media and inocula used, to an extent which varied with the antibiotic class ...19892681116
interactions in vitro between agents used to treat melioidosis.combinations of antimicrobial agents are usually recommended for the treatment of melioidosis. in thailand, the conventional treatment regimen for pseudomonas pseudomallei infections is a combination of chloramphenicol, doxycycline and cotrimoxazole. we have consistently observed antagonism between these agents during routine disc susceptibility testing. interactions between these antimicrobials were studied further by a chequerboard microdilution method, using five clinical isolates of p. pseud ...19892681117
melioidosis: a major cause of community-acquired septicemia in northeastern thailand.in a prospective study of all patients with pseudomonas pseudomallei infections admitted to a large provincial hospital in northeastern thailand, 63 cases of septicemic melioidosis and 206 patients with other community-acquired septicemias were documented during a 1-y period. apart from p. pseudomallei, the spectrum of bacteria isolated from blood cultures and the overall mortality (32%) were similar to those previously reported elsewhere. death from septicemia was associated with failure to dev ...19892708842
enzyme-linked immunosorbent assay for the diagnosis of clinical and subclinical melioidosis.an enzyme-linked immunosorbent assay (elisa) for the detection of specific igg and igm antibody to pseudomonas pseudomallei was developed. the igg-elisa was compared with the indirect fluorescence assay for igg antibody (igg-ifa) and the indirect hemagglutination (iha) test in studies with serum specimens from persons from endemic areas for melioidosis and from persons from nonendemic areas of australia. the sensitivity and specificity of the igg-elisa were 90% and 99%, respectively, comparable ...19892760482
multiple splenic abscesses following pseudomonas sepsis.splenic abscess is an unusual clinical entity, which in most cases is secondary to metastatic spread of bacteria from a primary site of infection. before the antibiotic era, salmonella typhi was a common offending organism but now staphylococci and streptococci are predominant organisms. we report two rare cases of multiple splenic abscesses caused by pseudomonas infection. one case followed pseudomonas septicaemia and the other was secondary to pseudomonas pseudomallei osteomyletis of the femur ...19892774478
splenic abscess at siriraj hospital, thailand.splenic abscess is an unusual disease and may be presented either as a localized area of infection in the spleen or as a part of generalized sepsis. population-based autopsy studies have established the incidence of splenic abscess at between 0.2-0.7 per cent. an eleven-year retrospective study of cases of splenic abscess treated at siriraj hospital, a total of 9 cases, is presented. pseudomonas pseudomallei is the most frequent causative agent, found in one-third of the cases, especially if the ...19892809452
in-vitro susceptibility of pseudomonas pseudomallei to dna gyrase inhibitors. 19872824429
[characteristics of the conjugation transfer of the plasmids of p-1-group incompatibility to pseudomonas pseudomallei strains]. 19882854878
antibiotic resistance in pseudomonas pseudomallei. 19882896855
acute suppurative parotitis caused by pseudomonas pseudomallei in children.during a prospective clinical study of melioidosis in northeast thailand, suppurative parotitis was observed as a characteristic presentation in children. parotitis constituted 6.3% of all culture-positive melioidosis and 38% of melioidosis in children. nine cases are described. none had apparent predisposition to infection, although two patients developed rising mumps virus antibody titers, suggesting a possible relation between these conditions. complications included abscess formation (nine), ...19892926159
in vitro susceptibility of strains of pseudomonas pseudomallei isolated in thailand and hong kong to some newer beta-lactam antibiotics and quinolone derivatives. 19862934486
[prospects of the use of chromosomal transformation for the identification of the causative agent melioidosis].prospects for the use of chromosomal transformation for the identification of pseudomonas pseudomallei and some other medically important pseudomonas species are considered. the method is simple, reproducible and highly specific. it permits the screening of a great number of cultures and can facilitate further study of the biology of p. pseudomallei and the epidemiology of melioidosis.19883057775
pseudomonas pseudomallei and melioidosis, with special reference to the status in thailand.melioidosis is a long-known disease since 1912, but only quite recently we have obtained the knowledges about its actual clinical and epidemiological features. the disease is so unique in having a wide spectrum of disease course and clinical manifestation. the causative agent, p. pseudomallei, is free-living bacterium in the natural environments (soil and surface water) of tropical and subtropical areas. just like legionnaires' disease, melioidosis is a good example of infectious disease in whic ...19883075004
[automated micromethod for the determination of the utilization of carbon sources by clinically significant pseudomonas species].the assimilation of 43 different carbon substrates by 93 clinical strains of pseudomonas aeruginosa was studied by a new miniaturized rapid method. reading of assimilation results was done photometrically after 18-20 h incubation and the resulting data were captured and stored by a microcomputer. the differentiating capacity of the assimilation tests were verified by comparing the results of 41 strains of pseudomonas fluorescens, 48 strains of pseudomonas putida, 52 strains of pseudomonas maltop ...19873118596
susceptibility of pseudomonas pseudomallei to new beta-lactam and aminoglycoside antibiotics.pseudomonas pseudomallei is resistant to many antimicrobial agents. we determined the susceptibility to twelve beta-lactams and six aminoglycosides of twelve isolates of p. pseudomallei. imipenem (mic range 0.5-1.0 mg/l; mic90 1.0 mg/l), ceftazidime (mic range 1-8 mg/l; mic90 8.0 mg/l), amoxycillin/clavulanate (mic range 4-8 mg/l; mic90 8.0 mg/l), piperacillin (mic range 4-16 mg/l; mic90 8.0 mg/l), and carumonam (mic range 4-16 mg/l; mic90 8.0 mg/l) were the most active. aminoglycosides were rel ...19883162898
neonatal melioidosis: a report of 5 cases.melioidosis, caused by pseudomonas pseudomallei, occurs in tropical areas and is diagnosed mostly in adults. in khon kaen, a province of northeast thailand, five cases of infantile melioidosis were managed at srinagarind hospital. the patient's specimens were submitted to microbiologic and serologic examination for p. pseudomallei demonstrated by indirect hemagglutination. possible modes of transmission such as environment, perinatal exposure and venereal transmission were investigated.19883174307
melioidosis with brain abscess.central nervous system involvement in melioidosis is rare. we describe a 48 year old woman who developed septicaemia and a brain abscess due to pseudomonas pseudomallei. since there is a continuing practical problem in bacteriological confirmation of the aetiological agent, diagnosis of melioidosis has to be made on clinical suspicion.19883174527
evaluation of four serological tests for the diagnosis of caprine melioidosis.a complement fixation (cf) test, 2 indirect haemagglutination (iha-a; iha-l) tests which differed in antigen preparation and technique, and a microtitre agglutination (ma) test were compared in the serodiagnosis of melioidosis in goats. one hundred and eighteen experimental serums and 3143 field serums from goats in endemic and non-endemic areas of north queensland were used in the evaluation. culture of samples for pseudomonas pseudomallei from 112 goats provided substantiating evidence of infe ...19883190597
resistance of pseudomonas pseudomallei to normal human serum bactericidal action.the effect of human normal serum (hns) on pseudomonas pseudomallei was determined. it is apparent from our data that the organism is resistant to the normal serum bactericidal mechanism. ancillary experiments to confirm this serum-resistant property of p. pseudomallei were done by examining the effects of growth phase conditions of the bacteria (i.e., logarithmic and stationary phases) and different buffered systems used as diluent in our bactericidal assay. results obtained showed similar degre ...19883193910
in vitro activities of the newer beta-lactam and quinolone antimicrobial agents against pseudomonas pseudomallei.imipenem was highly active and bactericidal against all 100 strains of pseudomonas pseudomallei tested, followed in activity by piperacillin, carumonam, ceftazidime, cefotaxime, and ceftriaxone. the addition of clavulanic acid significantly increased the activities of both amoxicillin and ticarcillin. ciprofloxacin and norfloxacin showed poor activity against test strains.19883196005
childhood melioidosis in northeastern thailand.eighteen cases of childhood melioidosis in northeastern thailand were reviewed. the mean age was 6.8 years with a range from eight months to 15 years. twelve cases (66.7%) had localized melioidosis, six of which had pneumonia. three patients were diagnosed as pharyngocervical melioidosis, the newly recognized syndrome. nine cases (50.0%) had associated diseases including dengue hemorrhagic fever (dhf) in five cases. in all five cases, melioidosis was diagnosed during the convalescent stage as a ...19883227408
characterization of monoclonal antibodies to protein antigen of salmonella typhi.two monoclonal antibodies were produced against protein antigens of salmonella typhi. one of the antibodies (stp14) belongs to the immunoglobulin g1k subclass, and the other (stp13) was assigned to the immunoglobulin g2a(kappa) subclass. both antibodies could recognize the 34.0-kilodalton protein antigen from s. typhi. the specificity of these antibodies was tested by immunoblotting with a panel of crude protein antigens from 12 bacteria causing enteric fever and enteric fever-like illness: s. t ...19883281976
clinical and pathological observations on goats experimentally infected with pseudomonas pseudomallei.the effects in goats of the subcutaneous injection of varying doses of pseudomonas pseudomallei (90 to 500,000 bacilli) suspended in normal saline are described. high doses (greater than or equal to 500 bacilli) caused acute, fatal infections. lower doses (90 to 225 bacilli) caused acute or chronic disease when infection became established. however, 11 of 18 goats injected with the lower doses of bacilli showed no sign of infection on clinical or bacteriological examination. response to antibiot ...19883355451
melioidosis: a reminder.recrudescent pulmonary melioidosis developed in two patients 12 and 16 years after their last travels to an endemic area. in one, a clinically silent prostatic abscess may have been the focus; and in both, the diagnosis was difficult to make even when the laboratory was notified of the possibility of infection with pseudomonas pseudomallei. recrudescent melioidosis should be considered in febrile patients who have been in endemic areas regardless of the interval from last exposure to the develop ...19883364451
[septic melioidosis following a visit to india].a case is reported of lethal septicemic melioidosis due to pseudomonas pseudomallei in a 40-year-old woman who had been in india. the epidemiology, clinical findings and management of this unusual disease are discussed. the diagnostic value of serological tests and in vitro sensitivity of pseudomonas pseudomallei to various antibiotics are outlined. melioidosis should be considered in the differential diagnosis of any febrile condition in a person returning from a tropical country.19883375792
[ecological and biological maintenance of sapronoses exemplified by melioidosis].the growth and death of pseudomonas pseudomallei, the causative agent of melioidosis, in the soil and the antigenic properties of this microorganism in the soil, in culture media, and in the body of animals have been studied. as revealed in this study, p. pseudomallei can grow in nonsterile soil substrates without the loss of virulence and changes in its antigenic structure. in the body of animals this microorganism rapidly adapts its virulence to host species by the transformation of its antige ...19883389005
activities of five new fluoroquinolones against pseudomonas pseudomallei.thirty-four isolates of pseudomonas pseudomallei were tested by a broth microdilution technique for susceptibility to amifloxacin, ciprofloxacin, enoxacin, norfloxacin, and ofloxacin. ciprofloxacin was the most active agent tested, with an mic for 90% of the strains tested of 8 micrograms/ml. these in vitro results suggest that the fluoroquinolones tested would not be optimal for therapy of melioidosis.19883415212
antimicrobial susceptibility of pseudomonas pseudomallei. 19873440775
brain abscess due to pseudomonas pseudomallei.a brain abscess due to pseudomonas pseudomallei is presented. central nervous system involvement in melioidosis is rare and generally occurs in association with disseminated infection. the importance of early diagnosis and prompt treatment with prolonged antibiotic therapy is emphasised.19863458450
pseudomonas pseudomallei (melioidosis): acute septicemia and meningitis in patient with systemic lupus erythematosus. 19863463317
in-vitro susceptibility of pseudomonas pseudomallei isolated in malaysia to some new cephalosporins and a quinolone.the current drugs recommended for treatment of melioidosis are tetracycline, chloramphenicol and cotrimoxazole. unfortunately these drugs are not the drug of choice in an acutely ill patient with septicaemia prior to the availability of laboratory results. with the discovery of the new cephalosporins which have a broad spectrum of activity clinicians are using them either alone or in combination with other antibiotics in such critical situations. hence, an in-vitro study was carried out on the s ...19873477872
beta-lactamase of pseudomonas pseudomallei and its contribution to antibiotic resistance.beta-lactamase production was examined in nine strains of pseudomonas pseudomallei isolated from human, animal and environmental sources in thailand and hong kong. all produced the same weakly inducible, membrane associated chromosomal cephalosporinase, which had a molecular weight of 29,500 and an isoelectric point of 7.4-7.7. the enzyme resembled the cefuroximases of ps. cepacia and proteus vulgaris, but differed from the class i cephalosporinases typical of ps. aeruginosa and most enterobacte ...19873500161
melioidosis: a serological survey in a tuberculosis sanatorium in hong kong.a serological survey of 275 chinese patients with underlying pulmonary diseases in a tuberculosis sanatorium in hong kong showed that 39 (14%) had haemagglutinating antibody (ha) against pseudomonas pseudomallei in a titre of 1: 80 or above. only 9 of these 39 patients had travelled to endemic areas, suggesting that at least 30 patients (11%) had been exposed to ps. pseudomallei locally. females are affected as often as males, and the seropositive rate is the same whether patients are immunosupp ...19873503401
a competitive immunosorbent assay for detection of pseudomonas pseudomallei exotoxin.the development of monoclonal antibody and enzyme-linked immunosorbent assay (elisa) techniques has made possible the detection of specific antigens at extremely low concentrations. diagnosis of recalcitrant diseases such as melioidosis depends upon either early isolation and identification of the causative organism or the identification of a specific marker antigen, pseudomonas pseudomallei exotoxin, in serum; the latter is better because it allows more rapid and simple diagnosis. a method of d ...19873585969
subacute pulmonary melioidosis in a temperate climate.previous reports of cases of melioidosis that were seen in nonendemic areas of australia describe recrudescences of latent infection. we describe the case of a patient who presented in the cooler climate of melbourne with a probable primary, subacute pulmonary infection with pseudomonas pseudomallei. this case illustrates several points that bear consideration in the management of atypical pneumonia and, more specifically, pulmonary melioidosis. historical and occupational clues are easily misse ...19873600459
melioidosis in intensive piggeries in south eastern queensland.the epidemiology of melioidosis was investigated in 8 intensive piggery units which used water from the same river in south eastern queensland. in 3 consecutive years cases of disease followed heavy rainfall and flooding. although pseudomonas pseudomallei was not isolated from water or soil samples the water supply was suspected as the source of infection. affected pigs were detected at slaughter by the presence of abscesses most commonly in the bronchial lymph nodes (40%) and spleen (34%). one ...19863753342
naturally occurring melioidosis in a colonized rhesus monkey (macaca mulatta).an aged wild-caught male rhesus monkey (macaca mulatta), maintained in a research facility for 10 years, developed bilateral pelvic limb paralysis without other signs of disease. unresponsive to therapy, the monkey was killed and necropsied. chronic inflammation with osteolysis of thoracic vertebrae 10-13 was observed. pseudomonas pseudomallei was cultured and identified from cerebrospinal fluid obtained at the site of the thoracic lesion. this gram-negative bacterium can cause infection in anim ...19863773432
a case of melioidosis presenting with prostatic abscess in hong kong.melioidosis is endemic in southeast asia but reports of prostatic involvement are rare. we describe a patient with pseudomonas pseudomallei septicemia and a prostatic abscess who presented with signs and symptoms of a urinary tract infection. the organism was cultured from the blood, urine and prostatic pus, and was treated successfully with co-trimoxazole and surgical drainage. genitourinary infection with pseudomonas pseudomallei should be considered in patients from endemic areas, and bacteri ...19873795353
septic melioidosis after a visit to southeast asia.a case is reported of a fifty-seven year old man with fever, who was admitted to hospital after a recent visit to southeast asia. among the clinical findings prostatitis and broncho-pneumonia were noted. within twenty-four hours irreversible fulminant sepsis developed although he was treated with cefotaxime, tobramycin and erythromycin. post mortem pseudomonas pseudomallei was cultured from blood and aspirate collected by bronchoscopy. it is important to consider melioidosis as a cause of septic ...19863803377
[spontaneous genetic transformation in pseudomonas pseudomallei].spontaneous genetic transformation has been registered in pseudomonas pseudomallei cells. transforming frequencies registered reach 10(-4)-10(-5). spontaneous transformation has been simulated for pseudomonas pseudomallei in soil. intrageneric spontaneous transformation has been demonstrated to occur between pseudomonas pseudomallei and pseudomonas mallei.19853842757
pseudomonas pseudomallei in southern thailand.the distribution of pseudomonas pseudomallei was studied in soil and water from various sources of songkla province, southern thailand. p. pseudomallei was isolated from the surface soil of rubber plantations (60.9%) and from the bottom sediments of rice fields (78.1%). farmers and plantation workers will have a greater risk of contracting melioidosis.19854023820
pseudomonas pseudomallei infection from drowning: the first reported case in taiwan.we report a case of pseudomonas pseudomallei infection, in which the patient acquired the bacteria by aspiration of river water after a drowning incident near manila, the philippines. the pulmonary form of melioidosis was noted at the onset, but septicemia developed at a later stage. positive blood cultures were obtained 17 days after the accident. the patient was treated successfully with a combination of amikacin and cephalothin. this is the first report of p. pseudomallei infection documented ...19854044794
a case comparison of acquired immune deficiency syndrome (aids) in homosexual males with spindle-endothelial cell abnormalities and with recrudescent melioidosis.the aids syndrome includes cases of biopsy-proven kaposi's sarcoma in persons under 60 years of age, or biopsy- or culture-proven pneumocystis carinii pneumonia, or either of the life-threatening opportunistic infections in young previously healthy persons with no underlying cause of immunodeficiency (center for disease control criteria). here we described the first case of aids with early kaposi's sarcoma-like lesions in homosexual male drug addict and have compared the clinical and laboratory ...19854074477
acute pseudomonas pseudomallei pneumonia and septicemia following aspiration of contaminated water: a case report. 19854095613
melioidosis in malaysia. iv. intensive ecological study of carey island, selangor, for pseudomonas pseudomallei. 19694244150
morphological and cytological effects of carbenicillin on pseudomonas pseudomallei. 19744371050
inhibition of pseudomonas pseudomallei by carbenicillin, in vitro. 19744418167
pseudomonas pseudomallei: 1. infection in thailand. 19744499415
identification of pseudomonas pseudomallei. 19724552650
melioidoses, pseudomonas pseudomallei and the clinical laboratory. 19724556761
variable ammonia production among smooth and rough strains of pseudomonas pseudomallei: resemblance to bacteriocin production.the colonial morphology of some strains of pseudomonas pseudomallei was correlated with certain biochemical and physiological traits. after 3 days of growth on wahba or heart infusion agars, smooth-colony strains generated toxic amounts of ammonia. under the same conditions, the rough strains simultaneously produced oxalic acid which decreased the inhibitory concentration of ammonia. the ammonia-ammonium concentrations in smooth cultures exhibited certain bacteriocin-like characteristics. an unu ...19724562401
a serologic survey of military personnel and dogs in thailand and south vietnam for antibodies to arboviruses, rickettsia tsutsugamushi, and pseudomonas pseudomallei. 19724627553
[melioidosis and its causative agent: pseudomonas pseudomallei]. 19734703906
bactericidal synergism of novobiocin and tetracycline against pseudomonas pseudomallei. 19734726333
antibiotic susceptibility of pseudomonas pseudomallei.the in vitro activity of various antibiotics against pseudomonas pseudomallei was studied. the tetracyclines were the most active, followed by gentamicin, kanamycin, novobiocin, chloramphenicol, rifampin, cycloserine, erythromycin, and ampicillin.19734790939
pseudomonas pseudomallei (malleomyces pseudomallei) orchitis in a boar. 19744839140
selection and rapid identification of pseudomonas pseudomallei from other gram-negative bacteria. 19684871604
diagnostic criteria for differentiation of pseudomonads pathogenic for man.to determine the most useful diagnostic characters for the differentiation of pseudomonads pathogenic for man, including pseudomonas maltophilia (alcaligenes bookeri), p. stutzeri (bacillus denitrificans), p. pseudomallei (malleomyces pseudomallei), and apyocyanogenic strains of p. aeruginosa, a comparative examination was made of 58 strains of these bacilli for their morphological and biochemical characteristics. it was concluded that the criteria for differentiating these bacteria are type of ...19684879213
cross immunity between mycobacterial and pseudomonas pseudomallei infections in mice. 19714927632
identification of pseudomonas pseudomallei. 19714933030
[comparative action of malleomyces mallei, pseudomonas pseudomallei and pseudomonas aeruginosa on various nitrogen substances]. 19654953183
[thermostable antigens of pseudomonas pseudomallei and of malleomyces mallei and their characteristics in common]. 19674961924
[precipitating and agglutinating antigens of pseudomonas pseudomallei (whitmore's b.). i. thermostable and thermolabile complexes. serological typing]. 19704989883
[precipitating and agglutinating antigens of pseudomonas pseudomallei (whitmore's bacillus). ii. demonstration of precitating antigens common to yersinia pestis and pseudomonas pseudomallei]. 19704995342
growth of pseudomonas pseudomallei & ps. aeruginosa on petroleum fractions. 19714999149
pseudomonas pseudomallei in the surface water of singapore. 19715094428
focal encephalitis due to pseudomonas pseudomallei. 19715107344
in vitro susceptibility of strains of pseudomonas pseudomallei to rifampin.reported high activity of rifampin for pseudomonas pseudomallei could not be verified by extensive in vitro tests conducted with 31 recently isolated strains. minimal inhibitory concentrations of rifampin were 25 mug/ml for three strains and greater than 25 mug/ml for 28 strains. rifampin had relatively poor in vitro activity when compared with tetracycline drugs and chloramphenicol antibiotics now commonly used for treating melioidosis.19715111301
susceptibility in vitro and in vivo of pseudomonas pseudomallei to rifampin and tetracyclines.tests were performed on 64 strains of pseudomonas pseudomallei to compare rifampin, various tetracyclines, and other antibiotics for inhibitory activity in vitro. rifampin minimum inhibitory concentration (mic) values generally fell between 25 to 50 mug/ml. for deoxycycline, methacycline, tetracycline, and minocycline, mic means ranged from 1.3 to 2.7 mug/ml. delayed treatment tests in subacute mouse infections revealed a better rifampin activity than was expected from its weak activity in vitro ...19715111303
effect of gentamicin on pseudomonas pseudomallei. 19715126250
the sensitivity of pseudomonas pseudomallei to trimethoprim and sulphamethoxazole in vitro.twelve out of 14 strains of pseudomonas pseudomallei were inhibited in vitro by a combination of trimethoprim 1 mug per ml and sulphamethoxazole 20 mug per ml or by lower concentrations. of the remaining strains, one was marginally more resistant and one was distinctly so. in tests utilizing two of the sensitive strains, inhibitory concentrations of the individual drugs exerted a bactericidal effect when combined.19715139985
pseudomonas pseudomallei: susceptibility to chemotherapeutic agents. 19705197996
pseudomonas pseudomallei: isolation from a chronic sternal abscess. 19695352785
[multiplication and virulence of septicemic and blood-infective agents in the organism of the infected animals and in their cadavers. xvi. study on models of pseudomonas pseudomallei experimental infections]. 19695398234
[biological and biochemical studies of the growth of virulence of several septicemic and blood-infective agents. vi. dehydrogenase activity of pseudomonas pseudomallei with various virulence]. 19695398239
nucleic acid similarities among pseudomonas pseudomallei, pseudomonas multivorans, and actinobacillus mallei.annealing experiments on membrane filters were carried out with deoxyribonucleic acids (dna) from selected strains of the nomen-species of pseudomonas, actinobacillus, chromobacterium, and micrococcus, with the use of dna of pseudomonas pseudomallei and actinobacillus mallei as reference materials. under the usual conditions employed in these experiments, the results were not quantitatively reproducible. incorporation of dimethylsulfoxide (dmso) into the incubation medium greatly increased diffe ...19705438051
pseudomonas pseudomallei in a case of chronic melioidosis. 19705471230
laboratory evaluation of rifampin and other antimicrobial agents against selected strains of pseudomonas pseudomallei. 19705521374
[transaminases and virulence. i. pseudomonas pseudomallei variants with different virulence]. 19705526115
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