Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| phenotypic characterization of three clinical isolates of burkholderia pseudomallei in ceará, brazil. | burkholderia pseudomallei, the causative agent of melioidosis was found in a small cluster of cases in tejuçuoca, ceará, brazil. tests were carried out to determine its phenotypic characteristics: colony morphology on ashdown agar and macconkey agar, biochemical profile in conventional biochemical tests and api 20ne, arabinose assimilation and susceptibility testing by disk diffusion, comparing with data in the literature. this study confirms the presence of b. pseudomallei in brazil and describ ... | 2006 | 16699716 |
| environmental factors that affect the survival and persistence of burkholderia pseudomallei. | 2006 | 16980433 | |
| use of a variable amplicon typing scheme reveals considerable variation in the accessory genomes of isolates of burkholderia pseudomallei. | melioidosis, a disease caused by the bacterium burkholderia pseudomallei, is endemic in southeast asia and northern australia. we used suppression subtractive hybridization (ssh) to identify sequences that varied between two b. pseudomallei isolates from australia and determined the distribution of 45 ssh-derived sequences among a panel of b. pseudomallei and b. thailandensis isolates. sequences exhibiting variable prevalence were included in a variable amplicon typing (vat) scheme designed to s ... | 2006 | 16597858 |
| burkholderia pseudomallei rpos regulates multinucleated giant cell formation and inducible nitric oxide synthase expression in mouse macrophage cell line (raw 264.7). | burkholderia pseudomallei is the causative agent of melioidosis. this bacterium can invade and survive inside the phagocytic and nonphagocytic cells. after internalization, the bacteria can escape from the membrane-bound phagosome into the cytoplasm. internalised b. pseudomallei can also induce a cell-to-cell fusion, resulting in a multinucleated giant cell (mngc) formation. in the present study, we demonstrated that b. pseudomallei rpos null mutant was similar to its wild type parent in its abi ... | 2006 | 16524693 |
| [molecular typing of the burkholderia pseudomallei strains with different resistance to antibiotics]. | wild type strains of burkholderia pseudomallei, spontaneous mutants with high resistance to fluoroquinolones and ceftazidime, and tn5-induced mutants with reduced resistance level were studied using polymorphic and gene-specific dna fingerprinting. cluster analysis of genomic dna patterns obtained using pcr with arbitrary primer (5'-gtttcgctcc-3') and primer specific to the class i integrase intll gene (5'-cctcccgcacgatgatc-3') was performed. according to the dna pattern conformity, the distinct ... | 2006 | 16512603 |
| using real-time pcr to specifically detect burkholderia mallei. | burkholderia mallei is the causative agent of human and animal glanders and is a category b biothreat agent. rapid diagnosis of b. mallei and immediate prophylactic treatment are essential for patient survival. the majority of current bacteriological and immunological techniques for identifying b. mallei from clinical samples are time-consuming, and cross-reactivity with closely related organisms (i.e. burkholderia pseudomallei) is a problem. in this investigation, two b. mallei-specific real-ti ... | 2006 | 16585642 |
| melioidosis: insights into the pathogenicity of burkholderia pseudomallei. | burkholderia pseudomallei is a potential bioterror agent and the causative agent of melioidosis, a severe disease that is endemic in areas of southeast asia and northern australia. infection is often associated with bacterial dissemination to distant sites, and there are many possible disease manifestations, with melioidosis septic shock being the most severe. eradication of the organism following infection is difficult, with a slow fever-clearance time, the need for prolonged antibiotic therapy ... | 2006 | 16541135 |
| lipopolysaccharide heterogeneity among burkholderia pseudomallei from different geographic and clinical origins. | heterogeneous patterns were obtained for lipopolysaccharide (lps) from 1,327 burkholderia pseudomallei isolates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, silver staining, and immunoblot analysis. two lps serotypes (a and b) possessing different ladder profiles and a rough lps without ladder appearances were identified. all three lps types were antigenically distinct by immunoblotting. the predominant type a (97%) produced the lowest amount of biofilm. the two less common type ... | 2006 | 16525090 |
| endotoxin-like properties of a rhamnolipid exotoxin from burkholderia (pseudomonas) plantarii: immune cell stimulation and biophysical characterization. | here we report on the purification, structural characterization, and biological activity of a glycolipid, 2-o-alpha-l-rhamnopyranosyl-alpha-l-rhamnopyranosyl-alpha(r)-3-hydroxytetradecanoyl-(r)-3-hydroxytetradecanoate (rl-2,2(14)) produced by burkholderia (pseudomonas) plantarii. rl-2,2(14) is structurally very similar to a rhamnolipid exotoxin from pseudomonas aeruginosa and identical to the rhamnolipid of burkholderia pseudomallei, the causative agent of melioidosis. interestingly, rl-2,2(14) ... | 2006 | 16542152 |
| six cases of suppurative lymphadenitis caused by burkholderia pseudomallei infection. | melioidosis, an infection caused by burkholderia pseudomallei, is endemic in southeast asia and northern australia. it can have various presentations, ranging from acute septicaemia to chronic localized infection. uncomplicated, localized infection usually involves skin or subcutaneous tissues. suppurative lymphadenitis caused by melioidosis has been rarely encountered by clinicians practicing in endemic areas. we encountered six cases of suppurative lymphadenitis caused by burkholderia pseudoma ... | 2006 | 16542691 |
| in vitro antimicrobial activity of natural toxins and animal venoms tested against burkholderia pseudomallei. | burkholderia pseudomallei are the causative agent of melioidosis. increasing resistance of the disease to antibiotics is a severe problem in treatment regime and has led to intensification of the search for new drugs. antimicrobial peptides are the most ubiquitous in nature as part of the innate immune system and host defense mechanism. | 2006 | 16784542 |
| development of a polymerase chain reaction assay for the specific identification of burkholderia mallei and differentiation from burkholderia pseudomallei and other closely related burkholderiaceae. | burkholderia mallei and burkholderia pseudomallei, the etiologic agents responsible for glanders and melioidosis, respectively, are genetically and phenotypically similar and are category b biothreat agents. we used an in silico approach to compare the b. mallei atcc 23344 and b. pseudomallei k96243 genomes to identify nucleotide sequences unique to b. mallei. five distinct b. mallei dna sequences and/or genes were identified and evaluated for polymerase chain reaction (pcr) assay development. g ... | 2006 | 16546342 |
| clinical guideline for diagnosis and management of melioidosis. | melioidosis is an emerging infection in brazil and neighbouring south american countries. the wide range of clinical presentations include severe community-acquired pneumonia, septicaemia, central nervous system infection and less severe soft tissue infection. diagnosis depends heavily on the clinical microbiology laboratory for culture. burkholderia pseudomallei, the bacterial cause of melioidosis, is easily cultured from blood, sputum and other clinical samples. however, b. pseudomallei can be ... | 2006 | 16547571 |
| stent-graft for recurrent melioidosis mycotic aortic aneurysm. | melioidosis is a tropical disease caused by burkholderia pseudomallei and is prevalent in south east asia and northern australia. it can infect any organ system and is potentially deadly. melioidosis causing a mycotic aneurysm of the aorta is rare. we present a patient with a melioidosis mycotic aneurysm of the descending aorta presenting with fever and right pleural effusion, managed successfully with initial dacron graft repair with staged omental reinforcement, and subsequent endovascular ste ... | 2006 | 16551809 |
| actin-dependent movement of bacterial pathogens. | listeria, rickettsia, burkholderia, shigella and mycobacterium species subvert cellular actin dynamics to facilitate their movement within the host cytosol and to infect neighbouring cells while evading host immune surveillance and promoting their intracellular survival. 'attaching and effacing' escherichia coli do not enter host cells but attach intimately to the cell surface, inducing motile actin-rich pedestals, the function of which is currently unclear. the molecular basis of actin-based mo ... | 2006 | 16415925 |
| dural sinus thrombosis in melioidosis: the first case report. | melioidosis which is infection with burkholderia pseudomallei, is an important cause of sepsis in india, southeast asia and northern australia. mortality is high and treatment is problematic. neurological melioidosis is unusual but meningoencephalitis, encephalomyelitis and brain microabscess can occur dural sinus thrombosis is not an uncommon cerebrovascular disorder with various etiologies. hypercoagulable state, pregnancy, dehydration, certain blood dyscrasia and contraceptive pills are commo ... | 2006 | 16579013 |
| computed tomography characteristics of burkholderia pseudomallei liver abscess. | we report 3 cases of burkholderia pseudomallei liver abscesses that have a characteristic "honeycomb" appearance on computed tomography scans. such a finding should prompt physicians to include b. pseudomallei infection in the differential diagnoses of liver abscesses and consider initiation of empirical therapy for melioidosis in high-risk patients from areas of endemicity. | 2006 | 16511765 |
| quorum sensing in the genus burkholderia. | the genus burkholderia contains over 30 species, many of which are important human pathogens. in addition to the primary pathogens burkholderia pseudomallei and burkholderia mallei, several species have emerged as opportunistic pathogens in persons suffering from cystic fibrosis (cf) and immunocompromised individuals. all burkholderia species investigated so far employ quorum-sensing (qs) systems that rely on n-acyl-homoserine lactone (ahl) signal molecules to express certain phenotypic traits i ... | 2006 | 16490397 |
| characterization of the mrgrs locus of the opportunistic pathogen burkholderia pseudomallei: temperature regulates the expression of a two-component signal transduction system. | burkholderia pseudomallei is a saprophyte in tropical environments and an opportunistic human pathogen. this versatility requires a sensing mechanism that allows the bacterium to respond rapidly to altered environmental conditions. we characterized a two-component signal transduction locus from b. pseudomallei 204, mrgr and mrgs, encoding products with extensive homology with response regulators and histidine protein kinases of escherichia coli, bordetella pertussis, and vibrio cholerae. | 2006 | 16893462 |
| genomic fingerprinting of burkholderia pseudomallei and b. mallei pathogens with dna array based on interspecies sequence differences obtained by subtractive hybridization. | the ability to rapidly and efficiently identify causative agents of dangerous human and animal diseases is a prerequisite to diagnosis, prophylaxis and therapy. such identification systems can be developed based on dna markers enabling differentiation between various bacterial strains. one source of these markers is genetic polymorphism. an efficient method for detecting the most stable polymorphisms without knowledge of genomic sequences is subtractive hybridization. in this work we report an a ... | 2006 | 16621455 |
| the innate interferon gamma response of balb/c and c57bl/6 mice to in vitro burkholderia pseudomallei infection. | burkholderia pseudomallei is the causative agent for melioidosis. for many bacterial infections, cytokine dysregulation is one of the contributing factors to the severe clinical outcomes in the susceptible hosts. the c57bl/6 and balb/c mice have been established as a differential model of susceptibility in murine melioidosis. in this study, we compared the innate ifn-gamma response to b. pseudomallei between the c57bl/6 and balb/c splenocytes and characterized the hyperproduction of ifn-gamma in ... | 2006 | 16919160 |
| serological evidence for increased human exposure to burkholderia pseudomallei following the tsunami in southern thailand. | a serological study was performed to determine recent human exposure to burkholderia pseudomallei (the cause of melioidosis) in residents of southern thailand affected by the tsunami of 26 december 2004. the findings were suggestive of increased recent exposure in both tsunami survivors and uninjured bystanders. survivors of the thailand tsunami may be at increased risk of melioidosis. | 2006 | 16390980 |
| identification of burkholderia pseudomallei genes required for the intracellular life cycle and in vivo virulence. | the bacterial pathogen burkholderia pseudomallei invades host cells, escapes from endocytic vesicles, multiplies intracellularly, and induces the formation of actin tails and membrane protrusions, leading to direct cell-to-cell spreading. this study was aimed at the identification of b. pseudomallei genes responsible for the different steps of this intracellular life cycle. b. pseudomallei transposon mutants were screened for a reduced ability to form plaques on ptk2 cell monolayers as a result ... | 2006 | 16714590 |
| genomic patterns of pathogen evolution revealed by comparison of burkholderia pseudomallei, the causative agent of melioidosis, to avirulent burkholderia thailandensis. | the gram-negative bacterium burkholderia pseudomallei (bp) is the causative agent of the human disease melioidosis. to understand the evolutionary mechanisms contributing to bp virulence, we performed a comparative genomic analysis of bp k96243 and b. thailandensis (bt) e264, a closely related but avirulent relative. | 2006 | 16725056 |
| role of selective and nonselective media for isolation of burkholderia pseudomallei from throat swabs of patients with melioidosis. | 2006 | 16757651 | |
| role of inducible nitric oxide synthase and nadph oxidase in early control of burkholderia pseudomallei infection in mice. | infection with the soil bacterium burkholderia pseudomallei can result in a variety of clinical outcomes, including asymptomatic infection. the initial immune defense mechanisms which might contribute to the various outcomes after environmental contact with b. pseudomallei are largely unknown. we have previously shown that relatively resistant c57bl/6 mice can restrict bacterial b. pseudomallei growth more efficiently within 1 day after infection than highly susceptible balb/c mice. by using thi ... | 2006 | 17000727 |
| development of antibodies to burkholderia pseudomallei during childhood in melioidosis-endemic northeast thailand. | a cross-sectional serological survey of 2,214 children living in northeast thailand was conducted to define the antibody response to burkholderia pseudomallei from birth to 14 years. there was a sharp rise in detectable antibodies from birth to 4 years followed by reactivity in approximately 60-70% of children thereafter. | 2006 | 16760522 |
| method for regulated expression of single-copy efflux pump genes in a surrogate pseudomonas aeruginosa strain: identification of the bpeef-oprc chloramphenicol and trimethoprim efflux pump of burkholderia pseudomallei 1026b. | construction and integration of recombinant mini-tn7 expression vectors into the chromosome of a surrogate, efflux-sensitized, and biosafe pseudomonas aeruginosa host was validated as a generally applicable method for studies of uncharacterized bacterial efflux pumps. using this method, the burkholderia pseudomallei bpeef-oprc operon was shown to encode a chloramphenicol and trimethoprim efflux pump. | 2006 | 17005832 |
| polysaccharide microarray technology for the detection of burkholderia pseudomallei and burkholderia mallei antibodies. | a polysaccharide microarray platform was prepared by immobilizing burkholderia pseudomallei and burkholderia mallei polysaccharides. this polysaccharide array was tested with success for detecting b. pseudomallei and b. mallei serum (human and animal) antibodies. the advantages of this microarray technology over the current serodiagnosis of the above bacterial infections were discussed. | 2006 | 16765554 |
| is a honeycomb appearance on computer tomography characteristic for burkholderia pseudomallei liver abscess? | 2006 | 16779761 | |
| genome-wide expression analysis of burkholderia pseudomallei infection in a hamster model of acute melioidosis. | burkholderia pseudomallei is the causative agent of melioidosis and represents a potential bioterrorism threat. in the current studies we have examined gene expression in b. pseudomallei in an animal model of acute melioidosis using whole-genome microarrays. gene expression profiles were generated by comparing transcriptional levels of b. pseudomallei-expressed genes in infected hamster organs including liver, lung, and spleen following intraperitoneal and intranasal routes of infection to those ... | 2006 | 16988221 |
| global map of growth-regulated gene expression in burkholderia pseudomallei, the causative agent of melioidosis. | many microbial pathogens express specific virulence traits at distinct growth phases. to understand the molecular pathways linking bacterial growth to pathogenicity, we have characterized the growth transcriptome of burkholderia pseudomallei, the causative agent of melioidosis. using a fine-scale sampling approach, we found approximately 17% of all b. pseudomallei genes displaying regulated expression during growth in rich medium, occurring as broad waves of functionally coherent gene expression ... | 2006 | 16997946 |
| melioidosis in an italian tourist injured by the tsunami in thailand. | 2006 | 17024805 | |
| a live experimental vaccine against burkholderia pseudomallei elicits cd4+ t cell-mediated immunity, priming t cells specific for 2 type iii secretion system proteins. | burkholderia pseudomallei is the etiological agent of melioidosis, a serious human disease for which no vaccine is available. immunization of susceptible balb/c mice with the live attenuated mutant b. pseudomallei ilvi (referred to as "2d2") generated significant, although incomplete, immunity. splenic b. pseudomallei-specific t cells, detected in immunized mice, proliferated and produced interferon-gamma in vitro in response to dead bacteria. assessment of t cell antigen specificity indicated t ... | 2006 | 17041850 |
| activity of tigecycline in the treatment of acute burkholderia pseudomallei infection in a murine model. | burkholderia pseudomallei is the causative agent of melioidosis. standard therapy includes ceftazidime alone or in combination with co-trimoxazole. tigecycline, a novel agent, has displayed activity against b. pseudomallei. we evaluated the in vivo efficacy of tigecycline using a murine model of melioidosis. mice were infected with either a high or low virulence b. pseudomallei isolate followed by administration of antibiotics alone or in combination (tigecycline, ceftazidime, tigecycline plus c ... | 2006 | 17046208 |
| cutaneous melioidosis. | melioidosis is an infection caused by the gram-negative bacterium burkholderia pseudomallei. this disease is endemic in southeast asia and north australia with sporadic occurrence in temperate countries, mostly imported from travellers. any organ can be involved in melioidosis whereby burkholderia pseudomallei causes an acute inflammatory reaction with rapid development of small abcesses which tend to coalesce to form larger abscesses. cutaneous manifestations vary greatly. we report a man with ... | 2006 | 17062053 |
| nonrandom distribution of burkholderia pseudomallei clones in relation to geographical location and virulence. | burkholderia pseudomallei is a soil-dwelling saprophyte and the causative agent of melioidosis, a life-threatening human infection. most cases are reported from northeast thailand and northern australia. using multilocus sequence typing (mlst), we have compared (i) soil and invasive isolates from northeast thailand and (ii) invasive isolates from thailand and australia. a total of 266 thai b. pseudomallei isolates were characterized (83 soil and 183 invasive). these corresponded to 123 sequence ... | 2006 | 16825379 |
| temperature-regulated microcolony formation by burkholderia pseudomallei requires pila and enhances association with cultured human cells. | burkholderia pseudomallei is the causative agent of melioidosis, a potentially fatal disease that is endemic to northern australia and southeast asia and is acquired from soil or water. adherence of b. pseudomallei 08 to cultured cells increases dramatically following prior growth at 30 degrees c or less compared to that following prior growth at 37 degrees c. here, we show that this occurs almost entirely as the result of microcolony formation (bacterium-bacterium interactions) following growth ... | 2006 | 16926432 |
| comparison by electron microscopy of intracellular events and survival of burkholderia pseudomallei in monocytes from normal subjects and patients with melioidosis. | burkholderia pseudomallei (b. pseudomallei) has been shown to persist intracellularly in patients with melioidosis, until reactivated by decreasing immunocompetence. we have previously demonstrated by transmission electron microscopy, the internalisation of b. pseudomallei by human macrophages and the occurrence of phagosome-lysosome fusion. | 2006 | 16865211 |
| pneumonia and septicemia caused by burkholderia thailandensis in the united states. | burkholderia thailandensis is closely related to burkholderia pseudomallei, the causative agent of melioidosis. it is generally considered avirulent and previously has been reported to occur only in southeast asia. we report the first case of pneumonia and septicemia caused by b. thailandensis in the united states. | 2006 | 17050819 |
| functional genetic analysis reveals a 2-alkyl-4-quinolone signaling system in the human pathogen burkholderia pseudomallei and related bacteria. | pseudomonas aeruginosa synthesizes diverse 2-alkyl-4(1h)-quinolones (ahqs), including the signaling molecule 2-heptyl-3-hydroxy-4(1h)-quinolone (pqs), via the pqsabcde locus. by examining the genome databases, homologs of the pqs genes were identified in other bacteria. however, apart from p. aeruginosa, only burkholderia pseudomallei and b. thailandensis contained a complete pqsa-e operon (termed hhqa-e). by introducing the b. pseudomallei hhqa and hhqe genes into p. aeruginosa pqsa and pqse mu ... | 2006 | 16873018 |
| development of mouse hybridomas for production of monoclonal antibodies specific to burkholderia mallei and burkholderia pseudomallei. | burkholderia mallei and b. pseudomallei are designated category b biothreat agents on the "select agents" list established by the nih and cdc. development of monoclonal antibodies (mabs) that could effectively differentiate these two closely related species of bacteria and other non-pathogenic burkholderia bacteria is urgently needed. splenocytes from mice immunized with various antigen preparations from either b. mallei (american type culture collection [atcc] 23344) or b. pseudomallei (atcc 23 ... | 2006 | 16934015 |
| crystallization and preliminary x-ray diffraction analysis of bipd, a virulence factor from burkholderia pseudomallei. | burkholderia pseudomallei, the causative agent of melioidosis, possesses a protein-secretion apparatus that is similar to those found in salmonella and shigella. a major function of these secretion systems is to secrete virulence-associated proteins into target cells of the host organism. the bipd gene of b. pseudomallei encodes a secreted virulence factor that is similar in sequence and most likely functionally analogous to ipad from shigella and sipd from salmonella. thus, the bipd protein is ... | 2006 | 16880550 |
| pyogenic liver abscess caused by burkholderia pseudomallei in taiwan. | pyogenic liver abscess in taiwan is a well-known disease entity, commonly associated with a single pathogen, klebsiella pneumoniae. melioidosis is an endemic disease in taiwan that can manifest as multiple abscesses in sites including the liver. we report three cases of liver abscesses caused by burkholderia pseudomallei. the first patient was a 54-year-old diabetic woman, who presented with liver abscess and a left subphrenic abscess resulting from a ruptured splenic abscess, co-infected with k ... | 2006 | 16935773 |
| multilocus sequence typing of historical burkholderia pseudomallei isolates collected in southeast asia from 1964 to 1967 provides insight into the epidemiology of melioidosis. | a collection of 207 historically relevant burkholderia pseudomallei isolates was analyzed by multilocus sequence typing (mlst). the strain collection contains environmental isolates obtained from a geographical distribution survey of b. pseudomallei isolates in thailand (1964 to 1967), as well as stock cultures and colony variants from the u.s. army medical research unit (malaysia), the walter reed army institute for research, and the pasteur institute (vietnam). the 207 isolates of the collecti ... | 2006 | 16891516 |
| expression, purification, crystallization and preliminary crystallographic analysis of bipd, a component of the burkholderia pseudomallei type iii secretion system. | a construct consisting of residues 10-310 of bipd, a component of the burkholderia pseudomallei type iii secretion system (t3ss), has been overexpressed as a gst fusion, cleaved from the gst tag and purified. crystals were grown of native and selenomethionine-labelled bipd. the crystals grow in two different polymorphs from the same condition. the first polymorph belongs to space group c222, with unit-cell parameters a = 103.98, b = 122.79, c = 49.17 a, a calculated matthews coefficient of 2.4 a ... | 2006 | 16946464 |
| clinical evaluation of a type iii secretion system real-time pcr assay for diagnosing melioidosis. | a burkholderia pseudomallei type iii secretion system real-time pcr assay was evaluated on clinical specimens in a region where melioidosis is endemic. the pcr was positive in 30/33 (91%) patients with culture-confirmed melioidosis. all six patients with melioidosis septic shock were blood pcr positive, suggesting potential for rapid diagnosis and commencement of appropriate therapy. | 2006 | 16891534 |
| high resolution structure of bipd: an invasion protein associated with the type iii secretion system of burkholderia pseudomallei. | burkoldheria pseudomallei is a gram-negative bacterium that possesses a protein secretion system similar to those found in salmonella and shigella. recent work has indicated that the protein encoded by the bipd gene of b. pseudomallei is an important secreted virulence factor. bipd is similar in sequence to ipad from shigella and sipd from salmonella and is therefore likely to be a translocator protein in the type-iii secretion system of b. pseudomallei. the crystal structure of bipd has been so ... | 2006 | 16950399 |
| imported melioidosis--south florida, 2005. | in 2005, two cases of melioidosis (one in august, one in october) were reported to the florida department of health, the first cases since reporting the disease became mandatory in florida in 2003. in one case, burkholderia pseudomallei was not recognized as the bacterium that causes the disease melioidosis, which led to a delay in reporting the case to the local health department. in both cases, delayed recognition and unsafe laboratory practices resulted in laboratory workers being exposed to ... | 2006 | 16915220 |
| in silico analysis of burkholderia pseudomallei genome sequence for potential drug targets. | recent advances in dna sequencing technology have enabled elucidation of whole genome information from a plethora of organisms. in parallel with this technology, various bioinformatics tools have driven the comparative analysis of the genome sequences between species and within isolates. while drawing meaningful conclusions from a large amount of raw material, computer-aided identification of suitable targets for further experimental analysis and characterization, has also led to the prediction ... | 2006 | 16922696 |
| antibiotic susceptibility of 50 clinical isolates of burkholderia pseudomallei from singapore. | 2006 | 16951412 | |
| burkholderia oklahomensis sp. nov., a burkholderia pseudomallei-like species formerly known as the oklahoma strain of pseudomonas pseudomallei. | c6786, the clinical isolate of the 'oklahoma' strain of pseudomonas (now burkholderia) pseudomallei, was originally isolated in 1973 from a wound infection resulting from a farming accident in oklahoma, usa. environmental isolates c7532 and c7533 from the oklahoma accident site were found to match c6786. these three isolates and a clinical isolate originally identified as b. pseudomallei that was recovered from a person in georgia, usa, involved in an automobile accident were characterized by bi ... | 2006 | 16957116 |
| melioidosis: an imported case from madagascar. | burkholderia pseudomallei, the causative agent of melioidosis, is endemic in southeast asia and northern australia but is a rare pathogen in other parts of the world. no human case of melioidosis has been reported in madagascar until now. we describe a case of pulmonary melioidosis probably acquired in madagascar. | 2006 | 16987131 |
| melioidosis: a potentially life threatening infection. | melioidosis is caused by the gram-negative bacillus, burkholderia pseudomallei. it is endemic in tropical australia and in southeast asian countries. the overall mortality from this infection remains extremely high despite recent advancement in its treatment. this review discuss about clinical manifestations, diagnosis and management of melioidosis. | 2006 | 17240600 |
| mycobacterium avium and burkholderia pseudomallei (melioidosis) coinfection in an hiv-positive patient. | a 29 year old hiv positive thai female with cd4 count of 10 cells/mm3 presented with chronic diffuse abdominal pain, fever, weight loss, anemia and leucopenia. ultrasonography demonstrated diffuse upper abdominal lymphadenopathy with ascites. microbiological and molecular work up of the specimen obtained by ultrasound-guided lymph node aspiration revealed co-infection with burkholderia pseudomallei and mycobacterium avium. indirect hemagglutination, igm-indirect fluorescent antibody, and igg-ind ... | 2006 | 17348247 |
| pcr-based identification of burkholderia pseudomallei. | dna amplification techniques are being used increasingly in clinical laboratories to confirm the identity of medically important bacteria. a pcr-based identification method has been in use in our centre for 10 years for burkholderia pseudomallei and was used to confirm the identity of bacteria isolated from cases of melioidosis in ceará since 2003. this particular method has been used as a reference standard for less discriminatory methods. in this study we evaluated three pcr-based methods of b ... | 2006 | 17086309 |
| computed tomography characteristics of burkholderia pseudomallei-associated liver abscess. | 2006 | 17109298 | |
| melioidosis in the americas. | melioidosis is a potentially severe bacterial infection caused by burkholderia pseudomallei. there has been growing awareness of the disease in the americas, particularly since the vietnam conflict when it was diagnosed in returning service personnel. accidental laboratory exposure indicates the difficulty making a culture-based diagnosis when melioidosis has not been considered in the differential diagnosis. melioidosis is most likely underdiagnosed in tropical central and south america where c ... | 2006 | 17123994 |
| management of melioidosis. | melioidosis is a serious human infection caused by the environmental gram-negative bacterium burkholderia pseudomallei. outcome following melioidosis remains poor despite 20 years of clinical research. overall mortality is 50% in north-east thailand (35% in children) and 19% in australia. relapse is common (13% over 10 years), and results from failure to eradicate the organism. treatment is required to complete 12-20 weeks, or longer if clinically indicated. this is divided into intravenous and ... | 2006 | 16771621 |
| melioidosis with endophthalmitis. | 2006 | 17030726 | |
| short report: melioidosis in myanmar: forgotten but not gone? | a serologic survey of adults resident in myanmar was conducted to define the presence of antibodies to burkholderia pseudomallei, the cause of melioidosis. antibodies were detectable by indirect hemagglutination assay (iha) in 757 (78%) of 968 adults, of whom 69 (7%) had an iha titer > or =1:160. | 2006 | 17123993 |
| in vitro motility of a population of clinical burkholderia pseudomallei isolates. | melioidosis, a serious infection caused by burkholderia pseudomallei, is a leading cause of community-acquired sepsis in northeast thailand, and the commonest cause of death from community-acquired pneumonia in the top end of northern australia. the causative organism is a gram-negative, motile bacillus that is a facultative intracellular pathogen. b. pseudomallei flagella have been proposed as a possible vaccine candidate and putative virulence determinant. flagella expression was highly conser ... | 2006 | 17100392 |
| the mvin homolog in burkholderia pseudomallei is essential for viability and virulence. | the virulence factors of burkholderia pseudomallei, the causative agent of melioidosis, are not fully understood. we have identified a gene with homology to the salmonella typhimurium mouse virulence gene, mvin, a member of the mouse virulence factor family. expression studies with an insertional mutant containing a lux operon demonstrated that the expression of the gene is influenced by free-iron availability in the media and by growth phase. the mutant displayed an increased ld50 value in the ... | 2006 | 17110975 |
| quorum sensing regulates dpsa and the oxidative stress response in burkholderia pseudomallei. | burkholderia pseudomallei is the causative agent of melioidosis, a fatal human tropical disease. the non-specific dna-binding protein dpsa plays a key role in protecting b. pseudomallei from oxidative stress mediated, for example, by organic hydroperoxides. the regulation of dpsa expression is poorly understood but one possibility is that it is regulated in a cell population density-dependent manner via n-acylhomoserine lactone (ahl)-dependent quorum sensing (qs) since a lux-box motif has been l ... | 2006 | 17159218 |
| fatal burkholderia pseudomallei septicaemia. | 2006 | 17180814 | |
| pharmacokinetic and pharmacodynamic assessment of co-amoxiclav in the treatment of melioidosis. | we conducted a prospective pharmacokinetic study of oral co-amoxiclav in patients with melioidosis to determine the optimal dosage and dosing interval in this potentially fatal infection. | 2006 | 17003061 |
| [cutaneous melioidosis: report of a case]. | 2006 | 17374271 | |
| extreme weather events and environmental contamination are associated with case-clusters of melioidosis in the northern territory of australia. | melioidosis, the infection due to the environmental organism burkholderia pseudomallei, is endemic to northern australia and south east asia. it is associated with exposure to mud and pooled surface water, but environmental determinants of this disease are poorly understood. we defined case-clusters in northern australia, determined their contribution to the observed rate of melioidosis, and explored clinical features and associated environmental factors. | 2006 | 16326823 |
| burkholderia pseudomallei infection in chronic granulomatous disease. | melioidosis is a severe illness caused by burkholderia pseudomallei, a sepsis-causing bacterial pathogen that is common in east asia. we reexamined the underlying diagnosis in a 12-year-old boy who was diagnosed in the french west indies with melioidosis when he was 4 years old. our investigations led to the determination of chronic granulomatous disease (cgd), an inherited condition characterized by phagocytic cell dysfunction. this is the third reported case of melioidosis associated with cgd ... | 2006 | 16328364 |
| prospective evaluation of a rapid immunochromogenic cassette test for the diagnosis of melioidosis in northeast thailand. | a prospective study was performed to compare a rapid immunochromogenic cassette test (ict) with the indirect haemagglutination assay (iha) and clinical rules for the diagnosis of melioidosis in an endemic area. the sensitivity and specificity of the igg ict was 86% and 47%, and the igm ict was 82% and 47%, respectively. these were similar to the results for iha (sensitivity 73%, specificity 64%) and clinical rules (73% and 37%). ict lacks clinical utility as a result of high background rates of ... | 2006 | 16168447 |
| immunogenicity and anti-burkholderia pseudomallei activity in balb/c mice immunized with plasmid dna encoding flagellin. | plasmid dna encoding the flagella protein (flagellin) was used as a vaccination candidate for the evaluation of its immunogenicity and for protection against infection with burkholderia pseudomallei. firstly, flagellin encoding plasmid dna was injected into balb/c mice intramuscularly and this elicited both a humoral and a cellular immune response. total igg production and the clonal expansion of the spleen cells increased in response to flagellin. the igg subclass response exhibited a dominance ... | 2006 | 16169637 |
| imported cutaneous melioidosis in traveler, belgium. | 2007 | 17582903 | |
| to catch a killer gene. | 2007 | 17380132 | |
| using box-pcr to exclude a clonal outbreak of melioidosis. | although melioidosis in endemic regions is usually caused by a diverse range of burkholderia pseudomallei strains, clonal outbreaks from contaminated potable water have been described. furthermore b. pseudomallei is classified as a cdc group b bioterrorism agent. ribotyping, pulsed-field gel electrophoresis (pfge) and multilocus sequence typing (mlst) have been used to identify genetically related b. pseudomallei isolates, but they are time consuming and technically challenging for many laborato ... | 2007 | 17603903 |
| control of quorum sensing by a burkholderia pseudomallei multidrug efflux pump. | the burkholderia pseudomallei khw quorum-sensing systems produced n-octanoyl-homoserine lactone, n-decanoyl-homoserine lactone, n-(3-hydroxy)-octanoyl-homoserine lactone, n-(3-hydroxy)-decanoyl-homoserine lactone, n-(3-oxo)-decanoyl-homoserine lactone, and n-(3-oxo)-tetradecanoyl-homoserine lactone. the extracellular secretion of these acyl-homoserine lactones is dependent absolutely on the function of the b. pseudomallei bpeab-oprb efflux pump. | 2007 | 17384185 |
| paediatric melioidosis with septic shock in a previously-well child. | we present a previously-healthy 12-year old girl from a rural community and who was admitted to a district general hospital in malaysia with coagulopathy and septic shock. despite receiving intensive care, she succumbed to her illness. blood cultures grew burkholderia pseudomallei. melioidosis is an unusual cause of paediatric gram-negative sepsis among children in malaysia. | 2007 | 17384864 |
| atp-binding cassette systems in burkholderia pseudomallei and burkholderia mallei. | atp binding cassette (abc) systems are responsible for the import and export of a wide variety of molecules across cell membranes and comprise one of largest protein superfamilies found in prokarya, eukarya and archea. abc systems play important roles in bacterial lifestyle, virulence and survival. in this study, an inventory of the abc systems of burkholderia pseudomallei strain k96243 and burkholderia mallei strain atcc 23344 has been compiled using bioinformatic techniques. | 2007 | 17391530 |
| imported melioidosis with an isolated cutaneous presentation in a 90-year-old traveller from bangladesh. | melioidosis is a tropical disease caused by infection with the bacterium burkholderia pseudomallei. most cases present as an acute febrile illness with severe pneumonia and sepsis. sub-acute and late onset disease can also occur melioidosis has been diagnosed among travellers who contracted the disease while staying in endemic areas during the rainy season. we report a case of travel-associated b. pseudomallei cutaneous infection in a febrile 90-year-old woman with diabetes mellitus, with early ... | 2007 | 17402688 |
| rapid multiplex immunofluorescent assay to detect antibodies against burkholderia pseudomallei and taxonomically closely related nonfermenters. | an indirect immunofluorescent assay to detect antibodies against the lipopolysaccharide (lps) of burkholderia pseudomallei and taxonomically closely related species was developed with the luminex system. lpss of pseudomonas aeruginosa, burkholderia cepacia, burkholderia thailandensis, burkholderia vietnamiensis, b. pseudomallei, and burkholderia mallei were successfully conjugated to luminex microspheres. antibodies measured against the lps of b. pseudomallei-conjugated luminex beads only cross- ... | 2007 | 17642542 |
| use of protein-specific monoclonal antibody-based latex agglutination for rapid diagnosis of burkholderia pseudomallei infection in patients with community-acquired septicemia. | a latex agglutination test employing monoclonal antibody specific to a 30-kda protein of burkholderia pseudomallei was used to detect the organisms in blood culture specimens from 1,139 patients with community-acquired septicemia. the sensitivity, specificity, and positive and negative predictive values of the test were 96.75%, 99.61%, 96.75%, and 99.61%, respectively. | 2007 | 17428952 |
| assessing the reliability of eburst using simulated populations with known ancestry. | the program eburst uses multilocus sequence typing data to divide bacterial populations into groups of closely related strains (clonal complexes), predicts the founding genotype of each group, and displays the patterns of recent evolutionary descent of all other strains in the group from the founder. the reliability of eburst was evaluated using populations simulated with different levels of recombination in which the ancestry of all strains was known. | 2007 | 17430587 |
| use of immunoblotting as an alternative method for serogrouping leptospira. | leptospirosis is a worldwide zoonotic disease caused by a spirochaete bacterium, leptospira. serological detection of this micro-organism basically relies on a conventional microscopic agglutination test (mat), which has some limitations and disadvantages. in the present study, immunoblotting has been applied as an alternative method for differentiating serogroups and serovars of leptospires. leptospiral whole-cell lysates from a total of 26 serovars were subjected to immunoblotting using rabbit ... | 2007 | 17446278 |
| melioidosis presenting as genitourinary infection in two men with diabetes. | melioidosis is an infectious disease caused by burkholderia pseudomallei. it occurs predominantly in tropical regions. the manifestations are protean which include pneumonia, visceral abscesses, septic arthritis, osteomyelitis, acute suppurative and chronic granulomatous lesions with involvement of almost all organ systems. fulminant sepsis is much more common and is associated with high mortality. hence prompt recognition and early treatment is warranted. we report unusual presentations of urin ... | 2007 | 17495376 |
| human single-chain fv antibodies against burkholderia mallei and burkholderia pseudomallei. | much effort has been devoted to the development of mouse monoclonal antibodies that react specifically with burkholderia mallei and burkholderia pseudomallei for diagnostic and/or therapeutic purposes. our present study focused on the screening of a phage-displayed nonimmune human single-chain fv (scfv) antibody library against heat-killed b. mallei and b. pseudomallei for the generation of human scfv antibodies specific to the two pathogenic species of bacteria. using two different panning proc ... | 2007 | 17392491 |
| melioidosis presenting with isolated splenic abscesses: a case report. | splenic abscesses caused by burkholderia pseudomallei are rarely reported in taiwan. here we report a middle-aged man who presented with fever, chills, and general malaise for several days. abdominal echo revealed isolated splenic abscesses and he received antibiotics treatment according to the initial blood culture result, serratia marcescens. however, fever did not subside. then he was referred to our hospital and meropenem was prescribed. fever subsided 5 days after the beginning of meropenem ... | 2007 | 17666309 |
| a serratia marcescens oxyr homolog mediates surface attachment and biofilm formation. | oxyr is a conserved bacterial transcription factor with a regulatory role in oxidative stress response. from a genetic screen for genes that modulate biofilm formation in the opportunistic pathogen serratia marcescens, mutations in an oxyr homolog and predicted fimbria structural genes were identified. s. marcescens oxyr mutants were severely impaired in biofilm formation, in contrast to the hyperbiofilm phenotype exhibited by oxyr mutants of escherichia coli and burkholderia pseudomallei. furth ... | 2007 | 17675374 |
| melioidosis complicated by pericarditis. | melioidosis is a bacterial infection caused by the gram-negative bacillus burkholderia pseudomallei. we report an unusual case of melioidosis that presented as a pyrexia of unknown origin complicated by pericardial effusion. our patient received a 6-week course of intravenous antibiotics, followed by 8 months of oral antibiotics, and made a complete recovery. this report illustrates the diagnostic and therapeutic challenge that clinicians may encounter when faced with this potentially fatal infe ... | 2007 | 17454903 |
| characteristics of phenol biodegradation in saline solutions by monocultures of pseudomonas aeruginosa and pseudomonas pseudomallei. | phenol is a highly toxic and carcinogenic compound and its biodegradation is very important to meet the environmental regulations. two bacterial strains capable of utilizing phenol as a sole source of carbon were isolated from the wastewater of a pharmaceutical industry. on the basis of morphological and biochemical characteristics these strains were identified as pseudomonas aeruginosa and pseudomonas pseudomallei. both of these strains were very efficient for phenol degradation. p. pseudomalle ... | 2007 | 17459580 |
| liver abscess caused by tuberculosis and melioidosis. | we report an unusual co-existence of burkholderia pseudomallei and acid fast bacilli in a young malay gentleman with liver abscess. he was treated with antibiotics and surgical drainage. this phenomenon has not been reported in previous literature and the dilemma of its management is discussed. | 2007 | 17475585 |
| endogenous interleukin-18 improves the early antimicrobial host response in severe melioidosis. | melioidosis is caused by the soil saprophyte burkholderia pseudomallei and is endemic in southeast asia. the pathogenesis of melioidosis is still largely unknown, although gamma interferon (ifn-gamma) seems to play an obligatory role in host defense. previously, we have shown that ifn-gamma production in melioidosis is controlled in part by interleukin-18 (il-18). the aim of the present study was to determine the role of il-18 in the immune response to b. pseudomallei. for this the following inv ... | 2007 | 17517876 |
| [adequate selection of antigens for serologic diagnostics of experimental melioidosis]. | sera of burkholderia pseudomallei-infected golden hamsters, white mice, guinea pigs and white rats were studied. immunochemical analysis revealed presence of antibodies against antigens 2, 3, 6, d, and g with significant predominance of antibodies to antigens 6 and d. antigen d was detected irrespectively to strain used for experimental infection and experimental animal species. antibodies to antigen 6 were detected only when strains belonging to asian serovar, but not to australian serovar, wer ... | 2007 | 17523479 |
| melioidosis outbreak after typhoon, southern taiwan. | from july through september 2005, shortly after a typhoon, 40 cases of burkholderia pseudomallei infection (melioidosis) were identified in southern taiwan. two genotypes that had been present in 2000 were identified by pulsed-field gel electrophoresis. such a case cluster confirms that melioidosis is endemic to taiwan. | 2007 | 17553230 |
| [a japanese case of melioidosis presenting as multiple organ lesions accompanied by sepsis and disseminated intravascular coagulation, after a visit to thailand]. | a 65-year-old man with diabetes mellitus reporting fever and urination disturbance on a flight from bangkok back to japan in july 2003 was admitted elsewhere for acute prostatitis. despite intravenous antibiotics, his condition deteriorated. on admission to our hospital, he suffered from respiratory failure, with laboratory data showing disseminated intravascular coagulation (dic). computed tomography (ct) shows infiltrative and nodular shadows in both lung fields and low-density areas in the le ... | 2007 | 17564119 |
| fate of a burkholderia pseudomallei lipopolysaccharide mutant in the mouse macrophage cell line raw 264.7: possible role for the o-antigenic polysaccharide moiety of lipopolysaccharide in internalization and intracellular survival. | burkholderia pseudomallei is a facultative intracellular gram-negative bacterium that can survive and multiply inside macrophages. one of the mechanisms by which b. pseudomallei escapes macrophage killing is by interfering with the expression of inducible nitric oxide synthase (inos). however, the bacterial components that modulate antimicrobial activity of the macrophage have not been fully elucidated. in the present study, we demonstrated that b. pseudomallei strain srm117, a lipopolysaccharid ... | 2007 | 17576760 |
| isolated liver abscesses in melioidosis. | melioidosis is a suppurative chronic infection caused by a gramnegative bacterium, burkholderia pseudomallei. we report two patients who presented with isolated liver abscesses caused by this pathogen. both patients presented with high-grade fever and abdominal pain. on examination they were toxic and had tender hepatomegaly. investigations showed leucocytosis and a shift to the left. early diagnosis of melioidosis was made by culture and growth of burkholderia pseudomallei from aspirated pus fr ... | 2007 | 17582188 |
| prevalence of melioidosis in the er-ren river basin, taiwan: implications for transmission. | an increase in melioidosis cases compared to other areas in taiwan was observed in the er-ren river basin, southwestern taiwan, from november 2001 to august 2006. the objective of this study was to determine the association between the level of exposure to burkholderia pseudomallei and the incidence rate of melioidosis and to survey the transmission modes of b. pseudomallei in the er-ren river basin. the serosurveillance of melioidosis gave seropositivity rates of 36.6%, 21.6%, and 10.9%, respec ... | 2007 | 17596372 |
| accuracy of burkholderia pseudomallei identification using the api 20ne system and a latex agglutination test. | in an evaluation of the api 20ne for the identification of burkholderia spp., 792/800 (99%) burkholderia pseudomallei and 17/19 (89%) b. cepacia isolates were correctly identified but 10 b. mallei and 98 b. thailandensis isolates were not correctly identified. a latex agglutination test was positive for 796/800 (99.5%) b. pseudomallei isolates and negative for 120 other oxidase-positive gram-negative bacilli. | 2007 | 17804660 |
| melioidosis in a rural community of western province, papua new guinea. | a prospective study was conducted to determine the significance of melioidosis in the balimo district of western province, papua new guinea. during 1998, after the establishment of laboratory procedures and increasing local clinical awareness, the disease was found in 1.8% (95% ci 0.37-5.1%) of individuals presenting with fever refractory to standard treatment. the clinical incidence was 20.0 per 100,000 population (95% ci 12.2-30.9). the median age of culture-confirmed cases was 9.5 years (inte ... | 2007 | 17499321 |
| melioidotic septic arthritis: a case report and literature review. | burkholderia pseudomallei, the causative agent of melioidosis, is endemic in southeast asia and northern australia. in recent years, the incidence of melioidosis has increased worldwide. septic arthritis is a rare but well-recognized manifestation of melioidosis. patients with underlying medical conditions, such as diabetes mellitus, renal impairment, cirrhosis, and malignancy are at greater risk. the presentations of melioidotic septic arthritis often mimic other disease processes and patients ... | 2007 | 17446969 |
| sensitive and specific molecular detection of burkholderia pseudomallei, the causative agent of melioidosis, in the soil of tropical northern australia. | burkholderia pseudomallei, the cause of the severe disease melioidosis in humans and animals, is a gram-negative saprophyte living in soil and water of areas of endemicity such as tropical northern australia and southeast asia. infection occurs mainly by contact with wet contaminated soil. the environmental distribution of b. pseudomallei in northern australia is still unclear. we developed and evaluated a direct soil b. pseudomallei dna detection method based on the recently published real-time ... | 2007 | 17873073 |