Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| proteins of viral and cellular origin bind to the aleutian disease virus (adv) dna 3'-terminal hairpin: presentation of a scheme for encapsidation of adv dna. | we have observed the binding of viral and cellular proteins to the aleutian disease virus (adv) 3' terminus of replicative-form dna. gel retardation assays showed specific band shifts produced by whole-cell extracts from either adv-infected or uninfected cells, as well as band reduction produced by adv capsids. in all cases, binding was confined to the turnaround, t-shaped terminal form; no binding to the extended conformation of replicative-form dna was detected. this indicates the importance o ... | 1990 | 2157046 |
| analysis of experimental mink enteritis virus infection in mink: in situ hybridization, serology, and histopathology. | strand-specific hybridization probes were used in in situ hybridization studies to localize cells containing mink enteritis virus (mev) virion dna or mev replicative-form dna and mrna. following the experimental mev infection of 3-month-old unvaccinated mink, a significant increase in serum antibodies to mev was detected at postinfection day (pid) 6, 2 days after the onset of fecal shedding of virus. prior to the appearance of virus in feces, viral dna could be detected in the mesenteric lymph n ... | 1990 | 2159543 |
| pathogenesis of disease caused by aleutian mink disease parvovirus. | a review of the pathogenesis of aleutian mink disease parvovirus (adv) infection based on recent knowledge gained by the author and collaborators is given. the review focuses mainly on the following topics. 1) development of an easy, sensitive and fast assay for detection of adv antigens and antibodies directed against these antigens. a highly sensitive rocket line immunoelectrophoretic assay (rlie) was developed. this assay turned out to be 32 times more sensitive than the counter current elect ... | 1990 | 2159766 |
| nucleotide sequence of the 5'-terminal palindrome of aleutian mink disease parvovirus and construction of an infectious molecular clone. | the 5'-terminal palindrome of the adv-g strain of aleutian mink disease parvovirus (adv) was molecularly cloned and sequenced. a full-length molecular clone of adv-g, denoted pxvb, was then constructed. when this clone was transfected into cell cultures, infectious adv could be rescued. virus derived from pxvb was nonpathogenic for adult mink, as is the parent adv-g strain. | 1990 | 2161958 |
| violet mink develop an acute disease after experimental infection with aleutian disease virus (adv) isolate adv sl3. | six-aleutian (aa)-genotype violet mink were infected intraperitoneally with the aleutian disease virus (adv) bone marrow derived isolate adv sl3. all animals developed virus-specific antibodies and hypergammaglobulinaemia. mortality during the fourteen week duration of the infection was 50%. the virus induced (histo)pathological lesions typical for aleutian disease. by immunohistochemical examination using a virus capsid-specific monoclonal antibody viral antigen was detected in lymph nodes, spl ... | 1990 | 2163578 |
| nucleotide sequence analysis of aleutian mink disease parvovirus shows that multiple virus types are present in infected mink. | different isolates of aleutian mink disease parvovirus (adv) were cloned and nucleotide sequenced. analysis of individual clones from two in vivo-derived isolates of high virulence indicated that more than one type of adv dna were present in each of these isolates. analysis of several clones from two preparations of a cell culture-adapted isolate of low virulence showed the presence of only one type of adv dna. we also describe the nucleotide sequence from map units 44 to 88 of a new type of adv ... | 1991 | 1649336 |
| virus-specific b-lymphocytes are probably the primary targets for aleutian disease virus. | 368 1- to 5-year-old mink of wild-type or black genetic background were infected with aleutian disease virus (adv) naturally or using virus-containing immune complexes or purified virus. thirty of the mink were immunized with dinitrophenol-conjugated ovalbumin (dnp-oa) before and during infection. blood samples were taken at monthly intervals. we found that weak (and transient) monoclonal or oligoclonal immunoglobulin components were present in the plasma or serum approximately 1 month after inf ... | 1991 | 1651029 |
| investigation of mink mhc (mhcmuvi) class i molecules by isoelectric focusing (ief). | mink (mustela vision) class i leucocyte antigens, here abbreviated mhcmuvi according to the proposal given by klein et al. (1990), were characterized by isoelectric focusing (ief), using triton x-114-extracted and neuraminidase-treated membrane proteins from spleen cells, followed by immunoblotting and development with a murine monoclonal antibody raised against denatured major histocompatibility complex (mhc) class i antigens. muvi class i antigens were investigated in a group of 97 danish mink ... | 1991 | 1681893 |
| genetic polymorphism of igg in the mink. 7. expression of the c gamma-allotypes in domestic mink infected with the aleutian disease virus. | the aleutian disease (ad), i.e., viral plasmocytosis in mink can be used as a model of the natural development of immune complex pathology in man. the immunogenetic aspect of ad was studied with the help of genetic markers of the constant region of the mink immunoglobulin gamma-heavy chain (the c gamma allotypes h2, h3, h4, h6, h7 and h8). the frequencies of 2 of the 6 allotypes, h3 and h4, were significantly higher in the ad-infected than in normal minks from the same population. this supports ... | 1991 | 1789990 |
| replication of aleutian mink disease parvovirus in lymphoid tissues of adult mink: involvement of follicular dendritic cells and macrophages. | by using strand-specific in situ hybridization and immunohistochemistry, evidence for replication of the aleutian mink disease parvovirus was observed in cells resembling macrophages and cells resembling follicular dendritic cells at 10 days after infection but only in macrophages at 60 days. sequestration of the aleutian mink disease parvovirus in larger numbers of macrophages and follicular dendritic cells was noted at both 10 and 60 days. | 1991 | 1846208 |
| [activation of expression of two immunoglobulin sn-genes in the american mink with aleutian disease]. | 110 ranch-raised minks were injected with the aleutian disease virus. allotypes of constant regions of gamma-heavy chains of the mink immunoglobulins secreted have been analysed during 3 months. activation of the expression of two markers (h3 and/or h4) up to minor or to nominal level (above 200 micrograms/ml) was observed. no such enhancement of expression of two other allotypes (h6 and h8) was found. the results suggest that the expression of two mink immunoglobulin ch genes induced by viral i ... | 1991 | 1916255 |
| topographical analysis of the g virion of aleutian mink disease parvovirus with monoclonal antibodies. | the topography of the aleutian mink disease parvovirus (adv) g virion was analyzed with monoclonal antibodies and polyclonal antiserum. there was homology between the two major structural proteins as others have previously reported. trypsin treatment of the virion with subsequent immunoblotting revealed that vp2 represents the main peptide on the exterior of virion and that vp1 is probably embedded within the capsid. additional analyses of the trypsin-treated virions showed that vp2 is responsib ... | 1992 | 1280944 |
| follicular dendritic cell-b cell interactions in virus disease. common localization but different cell damage caused by antibody immobilized virus? | follicular dendritic cells (fdc) are involved in the trapping and retention of antigen-antibody complexes in lymphoid follicles. this fdc immobilized antigen is thought to be involved in the generation of memory b-lymphocytes. follicular trapping of both aleutian disease virus and hiv particles has been demonstrated. however as far as known their affects on fdc and follicular b-cells are completely different. it is hypothesized that the trapping of (antibody-complexed) virus particles by the fdc ... | 1992 | 1309642 |
| expression of aleutian mink disease parvovirus capsid proteins by a recombinant vaccinia virus: self-assembly of capsid proteins into particles. | a portion of a cdna clone containing coding sequences for both structural proteins (vp1 and vp2) of aleutian mink disease parvovirus (adv) was inserted into recombinant vaccinia viruses, vv:adsp. immunohistochemical staining of vv:adsp-infected cells revealed that the adv antigen was readily detected and localized in the nuclei of infected cells. analysis of vv:adsp-infected cell lystates indicated that both vp1 and vp2 were produced and comigrated with authentic vp1 and vp2 from adv-infected cr ... | 1992 | 1313919 |
| identification of aleutian mink disease parvovirus transcripts in macrophages of infected adult mink. | we examined aleutian mink disease parvovirus (adv) mrna expression in lymph nodes of adult mink infected with adv by northern (rna) blot and in situ hybridization. in northern blot analysis, adv transcripts were detected in the poly(a) rna fraction extracted from mesenteric lymph nodes of two of five mink 10 days after intraperitoneal inoculation with the virulent utah i strain of adv. in strand-specific in situ hybridization, adv dna and mrna were detected in some macrophagelike cells located i ... | 1992 | 1323697 |
| screening for antibodies against aleutian disease virus (adv) in mink. elucidation of dubious results by additive counterimmunoelectrophoresis. | in order to distinguish true positive results in counterimmunoelectrophoresis from false positive ones an additive counterimmunoelectrophoresis was developed. the method was tested on selected mink serum samples as part of a routine testing for antibodies towards aleutian disease virus on 3 million blood samples. the procedure of the method is, that a known positive serum sample is mixed with the patient serum to be tested. the result from a false positive sample will be one precipitin line towa ... | 1992 | 1335756 |
| nursing disease in mink: clinical and postmortem findings. | one hundred fifty lactating mink on one ranch in southern ontario were monitored over the lactation period in 1989 for evidence of clinical disease, and serial blood samples were collected for biochemical analysis. antemortem blood samples were collected and necropsies performed on the 17 females that developed nursing disease and on 17 controls matched to the same stage of lactation and on ten nonlactating controls. twenty-two additional nursing disease cases were selected from seven ranches in ... | 1993 | 8116144 |
| expression of aleutian mink disease parvovirus proteins in a baculovirus vector system. | we have previously published a detailed transcription map of aleutian mink disease parvovirus (adv) and proposed a model for the translation of the two virion structural proteins (vp1 and vp2) and three nonstructural proteins (ns-1, ns-2, and ns-3) (s. alexandersen, m. e. bloom, and s. perryman, j. virol. 62:3684-3994, 1988). to verify and further characterize this model, we cloned the predicted open reading frames for ns-1, ns-2, ns-3, vp1-vp2, and vp2 alone into a recombinant baculovirus and e ... | 1993 | 8380073 |
| comparison of promoter activity in aleutian mink disease parvovirus, minute virus of mice, and canine parvovirus: possible role of weak promoters in the pathogenesis of aleutian mink disease parvovirus infection. | aleutian mink disease parvovirus (adv) infection causes both acute and chronic disease in mink, and we have previously shown that it is the level of viral gene expression that determines the disease pattern. to study the gene regulation of adv, we have cloned the p3 adv and p36 adv promoters in front of a reporter gene, the chloramphenicol acetyltransferase (cat) gene, and analyzed these constructs by transient transfection in a feline kidney cell line and mouse nih 3t3 cells. the genes for adv ... | 1993 | 8383215 |
| cis-acting sequences in the aleutian mink disease parvovirus late promoter important for transcription: comparison to the canine parvovirus and minute virus of mice. | we are currently investigating the regulation of transcription of the aleutian mink disease parvovirus (adv). adv causes a chronic immune complex-mediated condition known as classical aleutian disease, characterized by slow viral replication. this slow replication is an intrinsic property of adv and distinguishes it from the more prototypic parvoviruses such as minute virus of mice (mvm) and canine parvovirus (cpv). we have previously suggested a role for the weak adv promoters in the slow repli ... | 1993 | 8383216 |
| aleutian mink disease parvovirus infection of mink peritoneal macrophages and human macrophage cell lines. | aleutian mink disease parvovirus (adv) mrnas are found in macrophages in lymph nodes and peritoneal exudate cells from adv-infected mink. therefore, we developed an in vitro infection system for adv by using primary cultures of mink macrophages or macrophage cell lines. in peritoneal macrophage cultures from adult mink, virulent adv-utah i strain showed nuclear expression of viral antigens with fluorescein isothiocyanate-labeled adv-infected mink serum, but delineation of specific viral proteins ... | 1993 | 8383229 |
| characterization of chimeric full-length molecular clones of aleutian mink disease parvovirus (adv): identification of a determinant governing replication of adv in cell culture. | the adv-g strain of aleutian mink disease parvovirus (adv) is nonpathogenic for mink but replicates permissively in cell culture, whereas the adv-utah 1 strain is highly pathogenic for mink but replicates poorly in cell culture. in order to relate these phenotypic differences to primary genomic features, we constructed a series of chimeric plasmids between a full-length replication-competent molecular clone of adv-g and subgenomic clones of adv-utah 1 representing map units (mu) 15 to 88. after ... | 1993 | 8396664 |
| aleutian mink disease parvovirus infection of mink macrophages and human macrophage cell line u937: demonstration of antibody-dependent enhancement of infection. | aleutian mink disease parvovirus (adv) infects macrophages in adult mink. the virulent adv-utah i strain, but not the cell culture-adapted adv-g strain, infects mink peritoneal macrophage cultures and the human macrophage cell line u937 in vitro. however, preincubation of adv-g with adv-infected mink serum enhanced its infectivity for u937 cells. the enhancing activity was present in the protein a-binding immunoglobulin g fraction in the serum, but f(ab')2 fragments failed to enhance the infecti ... | 1993 | 8230426 |
| pathogenesis of aleutian mink disease parvovirus infection: effects of suppression of antibody response on viral mrna levels and on development of acute disease. | we suppressed the b-cell development and antibody response in mink by using treatment with polyclonal anti-immunoglobulin m (anti-igm) to study the effects of antiviral antibodies on development of aleutian mink disease parvovirus (adv)-induced disease in more detail. newborn mink kits were injected intraperitoneally with 1 mg of either anti-igm or a control preparation three times a week for 30 to 34 days. at 21 days after birth, groups of mink kits were infected with the highly virulent united ... | 1994 | 8289377 |
| aleutian mink disease: puzzles and paradigms. | aleutian mink disease (ad) is a naturally occurring persistent virus infection of mink caused by the aleutian mink disease parvovirus (adv). the classical form of ad, which occurs in adult mink, is notable for high titers of antiviral antibodies, hypergammaglobulinemia, plasmacytosis, and immune complex disease. in addition, there is a progressive renal disease characterized by mesangial proliferative glomerulonephritis and severe interstitial nephritis. development of ad depends on both host an ... | 1994 | 7889316 |
| sequence comparison of the non-structural genes of four different types of aleutian mink disease parvovirus indicates an unusual degree of variability. | the present work shows that at least four different sequence types of aleutian mink disease parvovirus (adv) are present in adv isolates from mink. we here report the nucleotide sequences of these four types of adv from nucleotide 123 to 2208 (map unit 3 to 46). this part of the genome encodes three non-structural (ns) proteins of adv. comparison of the deduced amino acid sequences of these ns proteins showed that the adv proteins are much less conserved than the ns proteins from other members o ... | 1994 | 7998830 |
| expression of aleutian mink disease parvovirus capsid proteins in a baculovirus expression system for potential diagnostic use. | a 2.3-kb cdna clone encoding aleutian mink disease parvovirus (adv) structural proteins vp1 and vp2 was inserted into the polyhedron gene of autographa californica nuclear polyhedrosis virus (acnpv) and expressed by the recombinant virus, acadv-1, in spodoptera frugiperda-9 cells. sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western immunoblot analysis (wia) indicated that synthesis of both vp1 and vp2 was being directed by acadv-1. fluorescence microscopic examination of acadv- ... | 1994 | 8011776 |
| role of alveolar type ii cells and of surfactant-associated protein c mrna levels in the pathogenesis of respiratory distress in mink kits infected with aleutian mink disease parvovirus. | neonatal mink kits infected with aleutian mink disease parvovirus (adv) develop an acute interstitial pneumonia with clinical symptoms and pathological lesions that resemble those seen in preterm human infants with respiratory distress syndrome and in human adults with adult respiratory distress syndrome. we have previously suggested that adv replicates in the alveolar type ii epithelial cells of the lung. by using double in situ hybridization, with the simultaneous use of a probe to detect adv ... | 1994 | 8139047 |
| acute interstitial pneumonia in mink kits inoculated with defined isolates of aleutian mink disease parvovirus. | the present study addressed the causal role of aleutian mink disease parvovirus (adv) in acute interstitial pneumonia in mink kits. all the examined isolates of adv caused interstitial pneumonia in newborn kits, although the severity of disease and the mortality varied. these findings indicate that adv is the direct causal agent of this disease in mink kits and that cofactors, which could have been present in the original adv-k isolate, do not play a role. acute interstitial pneumonia characteri ... | 1994 | 8203085 |
| interstitial nephritis in aleutian mink disease. possible role of cell-mediated immunity against virus-infected tubular epithelial cells. | aleutian mink disease (ad) has been characterized by immune complex glomerulonephritis associated with persistent infection of aleutian mink disease parvovirus (adv). histopathological examination of kidneys from adv-infected mink in this study revealed that interstitial nephritis characterized by prominent damage of renal tubuli and lymphocyte infiltration was also common in ad along with glomerulonephritis. by using strand-specific in situ molecular hybridization technique, replication of adv ... | 1994 | 8203470 |
| purification and characterization of the major nonstructural protein (ns-1) of aleutian mink disease parvovirus. | we have previously described the expression of the major nonstructural protein (ns-1) of aleutian mink disease parvovirus (adv) in insect cells by using a baculovirus vector (j. christensen, t. storgaard, b. bloch, s. alexandersen, and b. aasted, j. virol. 67:229-238, 1993). to study its biochemical properties, adv ns-1 was expressed in sf9 insect cells and purified to apparent homogeneity with a combination of nuclear extraction, zn2+ ion chromatography, and immunoaffinity chromatography on mon ... | 1995 | 7853520 |
| the relationship between capsid protein (vp2) sequence and pathogenicity of aleutian mink disease parvovirus (adv): a possible role for raccoons in the transmission of adv infections. | aleutian mink disease parvovirus (adv) dna was identified by pcr in samples from mink and raccoons on commercial ranches during an outbreak of aleutian disease (ad). comparison of dna sequences of the hypervariable portion of vp2, the major capsid protein of adv, indicated that both mink and raccoons were infected by a new isolate of adv, designated adv-tr. because the capsid proteins of other parvoviruses play a prominent role in the determination of viral pathogenicity and host range, we decid ... | 1996 | 8551624 |
| investigation of the pathogenesis of transplacental transmission of aleutian mink disease parvovirus in experimentally infected mink. | the transplacental transmission of aleutian mink disease parvovirus (adv) was studied in experimental infection of 1-year-old female non-aleutian mink. the adv-seronegative female mink were inoculated with adv prior to mating or after the expected implantation of the embryos during pregnancy. a group of uninfected females served as a control group. animals from each group were killed prior to or shortly after parturition. the in situ hybridization technique with radiolabeled strand-specific rna ... | 1996 | 8627663 |
| subcellular localization of aleutian mink disease parvovirus proteins and dna during permissive infection of crandell feline kidney cells. | confocal microscopy allowed us to localize viral nonstructural (ns) and capsid (vp) proteins and dna simultaneously in cells permissively infected with aleutian mink disease parvovirus (adv). early after infection, ns proteins colocalized with viral dna to form intranuclear inclusions, whereas vp proteins formed hollow intranuclear shells around the inclusions. later, nuclei had irregular outlines and were virtually free of adv products. in these cells, inclusions of viral dna with or without as ... | 1996 | 8627805 |
| clusters of lymphoma in ferrets. | cluster outbreaks of lymphoma and leukemia have been associated with viral infections in many species including humans, cattle, and cats. this study describes epidemiological, clinical, and pathological features of cluster outbreaks of lymphoma in multiferret households and examines and compares the aleutian disease virus (adv) and feline leukemia virus (felv) status of cases, ferrets at risk, and controls. three ferret groups with 21 cases of histologically diagnosed lymphoma (12.6% cumulative ... | 1996 | 8630683 |
| identification of a dna segment in ferret aleutian disease virus similar to a hypervariable capsid region of mink aleutian disease parvovirus. | a 401bp dna fragment of ferret aleutian disease virus (adv) was amplified using pcr primers spanning a hypervariable region of mink adv capsid sequence. the amplified fragment was 88-89% homologous to the same region of previously known sequence of three different strains of mink adv, however, as low as 54% homology was observed when compared with a 39bp segment known as hypervariable region. within the predicted 13 amino acid hypervariable region, the ferret adv sequence differed at 6 positions ... | 1996 | 8774691 |
| a cluster of cases of juvenile mediastinal lymphoma in a ferret colony. | three cases of juvenile mediastinal lymphoma developed in a laboratory colony of ferrets. two ferrets became acutely moribund, and one was found dead with no preceding signs of illness. splenomegaly, hepatomegaly, and a large thoracic mass were the primary features in each case. all three ferrets had multiorgan metastasis of the tumor. two ferrets were tested for feline leukemia virus and aleutian disease virus with negative results. | 1996 | 8799931 |
| investigation of an outbreak of aleutian disease on a commercial mink ranch. | to determine the modes of transmission of aleutian mink disease in a natural outbreak. | 1996 | 8950422 |
| expression of aleutian mink disease parvovirus capsid proteins in defined segments: localization of immunoreactive sites and neutralizing epitopes to specific regions. | the capsid proteins of the adv-g isolate of aleutian mink disease parvovirus (adv) were expressed in 10 nonoverlapping segments as fusions with maltose-binding protein in pmal-c2 (pvp1, pvp2a through pvp2i). the constructs were designed to capture the vp1 unique sequence and the portions analogous to the four variable surface loops of canine parvovirus (cpv) in individual fragments (pvp2b, pvp2d, pvp2e, and pvp2g, respectively). the panel of fusion proteins was immunoblotted with sera from mink ... | 1997 | 8985402 |
| s-phase-dependent cell cycle disturbances caused by aleutian mink disease parvovirus. | we examined replication of the autonomous parvovirus aleutian mink disease parvovirus (adv) in relation to cell cycle progression of permissive crandell feline kidney (crfk) cells. flow cytometric analysis showed that adv caused a composite, binary pattern of cell cycle arrest. adv-induced cell cycle arrest occurred exclusively in cells containing de novo-synthesized viral nonstructural (ns) proteins. production of adv ns proteins, indicative of adv replication, was triggered during s-phase trav ... | 1997 | 8995664 |
| aleutian mink disease parvovirus infection of k562 cells is antibody-dependent and is mediated via an fc(gamma)rii receptor. | aleutian mink disease parvovirus (adv) infects macrophages but infection is restricted. using the human macrophage cell line u937, we reported infection to be antibody-dependent [22,23]. recently, we learned that the u937 cells were contaminated with the human cell line, k562 [12]. to clarify which cell line actually supported adv infection, we studied the antibody-dependent enhancement (ade) of adv infection in pure lines of u937 and k562. after infection with adv complexed with mink anti-adv a ... | 1997 | 9125049 |
| two parvoviruses that cause different diseases in mink have different transcription patterns: transcription analysis of mink enteritis virus and aleutian mink disease parvovirus in the same cell line. | the two parvoviruses of mink cause very different diseases. mink enteritis virus (mev) is associated with rapid, high-level viral replication and acute disease. in contrast, infection with aleutian mink disease parvovirus (adv) is associated with persistent, low-level viral replication and chronic severe immune dysregulation. in the present report, we have compared viral transcription in synchronized crfk cells infected with either mev or adv using a nonradioactive rnase protection assay. the ov ... | 1997 | 9188563 |
| analyses of leucocytes in blood and lymphoid tissues from mink infected with aleutian mink disease parvovirus (amdv). | mink were infected with aleutian mink disease parvovirus (amdv) and sacrificed at monthly intervals after infection. during this time humoral immune responses and leucocyte numbers in blood, mesenteric lymph node, spleen and thymus were monitored. serum hypergammaglobulinaemia was observed together with elevated antibody responses to amdv ns1 and vp1/2 proteins. in blood, a highly significant increase in cd8+ lymphocytes was observed. however, (presumed)cd4+ cells defined as cd3+cd8- cells, and ... | 1998 | 9656422 |
| vaccination with aleutian mink disease parvovirus (amdv) capsid proteins enhances disease, while vaccination with the major non-structural amdv protein causes partial protection from disease. | vaccination studies were performed with partially purified recombinant amdv vp1/2 capsids as well as with the major amdv non-structural protein (ns1). all vaccine constructs induced an antibody response, but did not prevent infection upon challenge with amdv. the severity of aleutian disease (ad) was judged by the serum gammaglobulin level, the quantity of peripheral blood cd8 lymphocytes, antibody titers to vp1/2 and ns1 proteins and mink death rates. the vp1/2 vaccine constructs enhanced the d ... | 1998 | 9682374 |
| a comparison between permissive and restricted infections with aleutian mink disease parvovirus (adv): characterization of the viral protein composition at nuclear sites of virus replication. | we used three-color fluorescent labeling and confocal microscopy to compare the permissive and the antibody-mediated, restricted replication of aleutian mink disease parvovirus (adv). in both permissive (crfk cells) and restricted (k562 cells) situations, both adv non-structural proteins (ns1 and ns2) concentrated at focal sites in the nucleus, which also contained viral dna. bromodeoxyuridine labeling demonstrated that these sites also supported active adv single-strand dna synthesis, indicatin ... | 1998 | 9784064 |
| construction of pathogenic molecular clones of aleutian mink disease parvovirus that replicate both in vivo and in vitro. | the adv-g isolate of aleutian mink disease parvovirus (adv) replicates permissively in crandell feline kidney (crfk) cells but is nonpathogenic for mink, whereas the highly pathogenic adv-utah isolate is nonviable in crfk cells. to assign control of host range in crfk cells and pathogenicity to specific regions of the adv genome, we constructed a full-length molecular clone chimeric between adv-g and adv-utah. if either the map unit (mu) 54-65 (clone g/u-5) or mu 65-88 (clone g/u-7) sections wer ... | 1998 | 9837793 |
| identification of a cell surface protein from crandell feline kidney cells that specifically binds aleutian mink disease parvovirus. | aleutian mink disease parvovirus (adv) is the etiological agent of aleutian disease of mink. the acute disease caused by adv consists of permissive infection of alveolar type ii cells that results in interstitial pneumonitis. the permissive infection is experimentally modeled in vitro by infecting crandell feline kidney (crfk) cells with a tissue culture-adapted isolate of adv, adv-g. adv-g vp2 empty virions expressed in a recombinant baculovirus system were analyzed for the ability to bind to t ... | 1999 | 10196278 |
| epitope mapping of aleutian mink disease parvovirus virion protein vp1 and 2. | six overlapping fragments of the aleutian mink disease parvovirus (amdv) virion protein vp1 and 2 (vp1/2) gene were inserted into the expression vector pmal-c2. four of the clones carried large overlapping fragments covering the entire vp1/2 gene. the remaining two clones covered specifically chosen regions within the vp1/2 gene. using a western blotting detection system, sera from amdv-infected mink were tested against the recombinant polypeptides. these studies showed reactions primarily direc ... | 1999 | 10219758 |
| three-dimensional structure of aleutian mink disease parvovirus: implications for disease pathogenicity. | the three-dimensional structure of expressed vp2 capsids of aleutian mink disease parvovirus strain g (advg-vp2) has been determined to 22 a resolution by cryo-electron microscopy and image reconstruction techniques. a structure-based sequence alignment of the vp2 capsid protein of canine parvovirus (cpv) provided a means to construct an atomic model of the advg-vp2 capsid. the advg-vp2 reconstruction reveals a capsid structure with a mean external radius of 128 a and several surface features si ... | 1999 | 10400786 |
| replication of aleutian mink disease parvovirus in vivo is influenced by residues in the vp2 protein. | aleutian mink disease parvovirus (adv) is the etiological agent of aleutian disease of mink. several adv isolates have been identified which vary in the severity of the disease they elicit. the isolate adv-utah replicates to high levels in mink, causing severe aleutian disease that results in death within 6 to 8 weeks, but does not replicate in crandell feline kidney (crfk) cells. in contrast, adv-g replicates in crfk cells but does not replicate in mink. the ability of the virus to replicate in ... | 1999 | 10482625 |
| molecular and structural basis of the evolution of parvovirus tropism. | parvoviruses have small genomes and, consequently, are highly dependent on their host for various functions in their reproduction. since these viruses generally use ubiquitous receptors, restrictions are usually intracellularly regulated. a lack of mitosis, and hence absence of enzymes required for dna replication, is a powerful block of virus infection. allotropic determinants have been identified for several parvoviruses: porcine parvovirus, canine parvovirus (cpv), feline parvovirus (feline p ... | 1999 | 10497831 |
| unusual, high genetic diversity of aleutian mink disease virus. | the genetic diversity of aleutian mink disease virus (amdv) was examined. sequences obtained from 35 clinical samples were compared with five published sequences. an unusual, high genetic variability was revealed. three phylogenetic subgroups of amdv were identified, and the presence of more than one genotype at some farms was detected. | 1999 | 10565948 |
| replication of aleutian mink disease parvovirus in mink lymph node histocultures. | aleutian mink disease parvovirus (adv), causes an immune disorder with a persistent infection of lymphoid organs in adult mink. we studied replication of adv in gel-supported histocultures prepared from adult mink mesenteric lymph node (mln). evidence of virus replication in the histocultures was first observed by indirect immunofluorescence 72 h after incubation with virus. cells resembling lymphocytes and macrophages contained both adv capsid (vp2) and nonstructural (ns1 and ns2) proteins, and ... | 2000 | 10644831 |
| nonsuppurative meningoencephalitis associated with aleutian mink disease parvovirus infection in ranch mink. | severe nonsuppurative meningoencephalitis associated with aleutian mink disease parvovirus (adv) infection was observed in adult ranch mink. brain lesions included severe, locally extensive to coalescing lymphoplasmacytic meningoencephalitis with accompanying gliosis, satellitosis, and mild extension of inflammation into the leptomeninges. adv was identified in mesenteric lymph node, spleen, brain, and liver of affected mink by polymerase chain reaction techniques. sequences of the adv isolate ( ... | 2000 | 10730948 |
| aleutian mink disease parvovirus in wild riparian carnivores in spain. | serious declines in populations of native european mink (mustela lutreola) have occurred in europe. one responsible factor may be infectious diseases introduced by exotic american mink (mustela vison). in order to investigate a possible role for aleutian mink disease parvovirus (adv), we surveyed native riparian carnivores and feral american mink. when serum samples from 12 free-ranging european and 16 feral american mink were tested, antibodies to adv were detected from three of nine european m ... | 2001 | 11272488 |
| [dynamic of dnase activity induction in serum of normal animals after heterogeneous dna injection and in hereditary pathology]. | studies of reactions in animals with hereditary diseases (sapphire minks highly sensitive to aleutian disease virus, adv; cba mice with 60-70% incidence of tumors; akr mice with 90% incidence of leukemia) showed that serum dnase activity in these animals dropped after injection of a foreign heterogeneous dna and remained decreased during 72 h. by contrast, serum dnase activity considerably and persistently increased after injection of dna in standard minks resistant to adv, c57bi/6j mice with 1% ... | 2001 | 11392967 |
| nucleotide sequence and polymerase chain reaction/restriction fragment length polymorphism analyses of aleutian disease virus in ferrets in japan. | two ferrets with spontaneous aleutian disease (ad) were found in japan. the diagnosis was verified by polymerase chain reaction (pcr) amplification of part of the capsid gene specific to ad virus (adv). the nucleotide sequences (365 bp in length) of the amplified fragments from the 2 ferrets differed by a single nucleotide, producing an amino acid alteration. compared with other types of adv, these isolates had 96% sequence similarity to a published ferret adv (fadv) in contrast to <91% homology ... | 2001 | 11478607 |
| identification of aleutian mink disease parvovirus capsid sequences mediating antibody-dependent enhancement of infection, virus neutralization, and immune complex formation. | aleutian mink disease parvovirus (adv) causes a persistent infection associated with circulating immune complexes, immune complex disease, hypergammaglobulinemia, and high levels of antiviral antibody. although antibody can neutralize adv infectivity in crandell feline kidney cells in vitro, virus is not cleared in vivo, and capsid-based vaccines have proven uniformly ineffective. antiviral antibody also enables adv to infect macrophages, the target cells for persistent infection, by fc-receptor ... | 2001 | 11602751 |
| caspase activation is required for permissive replication of aleutian mink disease parvovirus in vitro. | aleutian mink disease parvovirus (adv) is distinct among the parvoviruses as infection in vivo is persistent, restricted, and noncytopathic. in contrast, infections with other more prototypic parvoviruses, like mink enteritis virus (mev), are acute, cytopathic, and characterized by permissive replication in vivo. although apoptosis results in the death of cells acutely infected by parvoviruses, the role of apoptosis in adv infections is unknown. permissive infection of adv resulted in apoptosis ... | 2002 | 11878925 |
| caspase cleavage of the nonstructural protein ns1 mediates replication of aleutian mink disease parvovirus. | virus-induced apoptosis of infected cells can limit both the time and the cellular machinery available for virus replication. hence, many viruses have evolved strategies to specifically inhibit apoptosis. however, aleutian mink disease parvovirus (adv) is the first example of a dna virus that not only induces apoptosis but also utilizes caspase activity to facilitate virus replication. to determine the function of caspase activity during adv replication, virus-infected cell lysates or purified a ... | 2003 | 12692232 |
| cytokine profiles in adult mink infected with aleutian mink disease parvovirus. | the aim of this study was to examine the levels of gamma interferon (ifn-gamma)-, interleukin 4 (il-4)-, and il-8-producing cells in peripheral blood mononuclear cells from mink infected with the aleutian mink disease parvovirus (adv). as expected, adv-infected mink developed high plasma gamma globulin values (hypergammaglobulinemia) and enhanced quantities of cd8-positive (cd8(+)) cells in the blood during the infection. we quantified the percentages of ifn-gamma- and il-4-positive lymphocytes ... | 2003 | 12805443 |
| structure of adeno-associated virus serotype 5. | adeno-associated virus serotype 5 (aav5) requires sialic acid on host cells to bind and infect. other parvoviruses, including aleutian mink disease parvovirus (adv), canine parvovirus (cpv), minute virus of mice, and bovine parvovirus, also bind sialic acid. hence, structural homology may explain this functional homology. the amino acids required for cpv sialic acid binding map to a site at the icosahedral twofold axes of the capsid. in contrast to aav5, aav2 does not bind sialic acid, but rathe ... | 2004 | 15016858 |
| antibodies to aleutian mink disease parvovirus in free-ranging european mink (mustela lutreola) and other small carnivores from southwestern france. | owing to the rapid decline of the european mink (mustela lutreola) in france, a national conservation action plan has been initiated, in which scientific research to improve understanding of the causes of the decline is one of the primary objectives. in order to investigate the possible role of aleutian disease parvovirus (adv) in decline of the species, a serologic survey was conducted from march 1996 to march 2002 in 420 free-ranging individuals of six species of small carnivores distributed i ... | 2004 | 15465705 |
| dna vaccination with the aleutian mink disease virus ns1 gene confers partial protection against disease. | aleutian disease virus (adv) causes severe losses in mink. this happens in nature as well as in farms. in spite of several attempts to provide an efficient protective protein based vaccine, experiments have failed so far. only partial protection has been obtained. the aim of this work was to construct and test a protective dna vaccine based on the gene encoding for the adv non-structural protein 1 (ns1) and to test this construct as a potential vaccine candidate against adv infection or disease. ... | 2005 | 15652664 |
| structure of adeno-associated virus type 4. | adeno-associated virus (aav) is a member of the parvoviridae, belonging to the dependovirus genus. currently, several distinct isolates of aav are in development for use in human gene therapy applications due to their ability to transduce different target cells. the need to manipulate aav capsids for specific tissue delivery has generated interest in understanding their capsid structures. the structure of aav type 4 (aav4), one of the most antigenically distinct serotypes, was determined to 13-a ... | 2005 | 15795290 |
| two mink parvoviruses use different cellular receptors for entry into crfk cells. | mink enteritis virus (mev) and aleutian mink disease parvovirus (adv) are two mink parvoviruses that replicate permissively in crandell feline kidney (crfk) cells. we have used this cell model to examine if these two mink parvoviruses use the same cellular receptor. whereas the cellular receptor for mev is expected to be the transferrin receptor (tfr), the cellular receptor for adv has not been clearly identified. we used short hairpin rnas (shrnas) produced from plasmids to trigger rna interfer ... | 2005 | 16040076 |
| pathogenesis of aleutian mink disease parvovirus and similarities to b19 infection. | aleutian mink disease parvovirus (adv) is an unusual member of the autonomous parvoviruses in both its replication and pathogenesis. infection of newborn mink kits results in an acute disease typified by virus replication in type ii pneumocytes in the lung. this replication is permissive and cytopathic, characterized by the production of high levels of viral replicative intermediates and infectious progeny. however, infection of adult aleutian mink leads to a chronic form of the disease termed a ... | 2005 | 16316395 |
| persistent viral shedding during asymptomatic aleutian mink disease parvoviral infection in a ferret. | a 2-year-old domestic ferret that appeared clinically healthy was repeatedly seropositive for aleutian mink disease parvovirus (adv) over a 2-year observation period. antibody titers, determined by counter-immunoelectrophoresis, ranged from 1024 to 4096. viral dna also was identified in serum, urine, feces, and blood cell fractions by polymerase chain reaction analysis. ultimately, dna in situ hybridization revealed adv dna in histologic sections of various tissues and organs. these data indicat ... | 2005 | 16475522 |
| the transcription profile of aleutian mink disease virus in crfk cells is generated by alternative processing of pre-mrnas produced from a single promoter. | a reevaluation of the transcription profile of aleutian mink disease parvovirus (amdv)-infected crfk cells at either 32 degrees c or 37 degrees c has determined that strain amdv-g encodes six species of mrnas produced by alternative splicing and alternative polyadenylation of a pre-mrna generated by a single promoter at the left end of the genome. three different splicing patterns are used, and each type is found polyadenylated at either the 3' end of the genome (the distal site) or at a site in ... | 2006 | 16378968 |
| the abundant r2 mrna generated by aleutian mink disease parvovirus is tricistronic, encoding ns2, vp1, and vp2. | the abundant r2 mrna encoded by the single left-end promoter of aleutian mink disease parvovirus is tricistronic; it not only expresses the capsid proteins vp1 and vp2 but is also the major source for the nonstructural protein ns2. a cis-acting sequence within the ns2 gene was shown to be required for efficient capsid protein production, and its effect displayed a distinct location dependence. ribosome transit through the upstream ns2 gene region was necessary for efficient vp1 and vp2 expressio ... | 2007 | 17428872 |
| [genetic analysis of aleutian mink disease virus isolated in the primorye territory]. | the nucleotide sequence of the vp2 gene for aleutian mink disease virus isolated in the fur farms in the primorye territory (russia) was determined. the isolated strain was shown to be genetically close to highly pathogenetic american strains of the utah group. an intraspecific classification of adv is proposed. | 2007 | 17601051 |
| aleutian disease in two domestic striped skunks (mephitis mephitis). | this report describes the use of polymerase chain reaction and dna in situ hybridization to diagnose aleutian mink disease parvovirus dna in various tissue specimens from 2 companion striped skunks. clinical, laboratory, and microscopic findings also support a clinical diagnosis of aleutian disease in these mink. | 2007 | 17846242 |
| [prokaryotic expression and detective application of the main antigenic region of vp2 protein of aleutian mink disease parvovirus]. | to research safer diagnosis antigen for adv, the main antigenic region vp2a and vp2b gene of adv were obtained by restriction digestion of the recombinant plasmids pmd-vp2a and pmd-vp2b. then the genes were respectively cloned into pmal-c2 to get two prokaryotic recombinant plasmids pmal-vpa and pmal-vpb. the target genes were successfully expressed in the host cell tb1 when induced by iptg. the western blot analysis proved the recombinant proteins have good antigenic. the recombinant proteins w ... | 2007 | 18271270 |
| infection with aleutian disease virus-like virus in a captive striped skunk. | a 5-month-old captive female striped skunk (mephitis mephitis) was evaluated because of lethargy, signs of depression, azotemia, and erythema of the skin around the eyes. | 2008 | 18312184 |
| aleutian disease serology, protein electrophoresis, and pathology of the european mink (mustela lutreola) from navarra, spain. | the european mink, mustela lutreola, has suffered a dramatic decline in europe during the 20th century and is one of the most endangered carnivores in the world. the subpopulation of european mink from navarra, spain, estimated to number approximately 420, represents approximately two thirds of the total number of mink in spain. aleutian disease virus (adv) is a parvovirus with a high degree of variability that can infect a broad range of mustelid hosts. the pathogenesis of this virus in small c ... | 2008 | 18816991 |
| development and evaluation of an enzyme-linked immunosorbent assay based on recombinant vp2 capsids for the detection of antibodies to aleutian mink disease virus. | aleutian disease (ad), a common infectious disease in farmed minks worldwide, is caused by aleutian mink disease virus (amdv). serodiagnosis of ad in minks has been based on detection of amdv antibodies by counterimmunoelectrophoresis (cie) since the 1980s. the aim of this study was to develop and evaluate an enzyme-linked immunosorbent assay (elisa) based on recombinant virus-like particles (vlps) for identifying amdv antibodies from mink sera. amdv capsid protein (vp2) of a finnish wild-type s ... | 2009 | 19641102 |
| aleutian mink disease virus and humans. | reports of a possible relationship between aleutian mink disease parvovirus (amdv) and human infection are rare. however, 2 mink farmers with vascular disease and microangiopathy similar to that in mink with aleutian disease were found to have amdv-specific antibodies and amdv dna. these findings raise the suspicion that amdv may play a role in human disease. | 2009 | 19961696 |
| molecular epidemiology of aleutian mink disease virus in finland. | aleutian mink disease virus (amdv) is a parvovirus that causes an immune complex-mediated disease in minks. to gain a more detailed view of the molecular epidemiology of mink amdv in finland, we phylogenetically analysed 14 new finnish strains from 5 farms and all 40 strains with corresponding sequences available in genbank. a part of the major non-structural (ns1) protein gene was amplified and analysed phylogenetically. a rooted nucleotide tree was constructed using the maximum parsimony metho ... | 2009 | 18799272 |
| the capsid proteins of aleutian mink disease virus activate caspases and are specifically cleaved during infection. | aleutian mink disease virus (amdv) is currently the only known member of the genus amdovirus in the family parvoviridae. it is the etiological agent of aleutian disease of mink. we have previously shown that a small protein with a molecular mass of approximately 26 kda was present during amdv infection and following transfection of capsid expression constructs (j. qiu, f. cheng, l. r. burger, and d. pintel, j. virol. 80:654-662, 2006). in this study, we report that the capsid proteins were speci ... | 2010 | 20042496 |
| neuropathologic features of aleutian disease in farmed mink in ireland and molecular characterization of aleutian mink disease virus detected in brain tissues. | a neuropathologic survey was conducted on mink brains from the 5 licensed mink farms in ireland. the survey was part of a transmissible spongiform encephalopathy surveillance study. aleutian disease (ad) was present on 4 of the 5 farms (80%). neuropathologic features of nonsuppurative meningoencephalitis were common in mink from the 4 affected farms but were absent in the mink from the fifth farm, which was free of ad. the meningoencephalitis was characterized by infiltrates of lymphocytes and p ... | 2010 | 20093694 |
| a survey of aleutian mink disease virus infection of feral american mink in nova scotia. | spleen samples from 14 mink that were trapped in 4 counties of nova scotia were tested for the presence of the aleutian mink disease virus (amdv) by polymerase chain reaction. viral dna was not detected in samples from kings county (n = 2), but was detected in all the mink sampled from colchester (n = 2) and halifax (n = 6) counties, and 3 of 4 mink from yarmouth county. the high level of amdv-infected mink in colchester and halifax counties may pose a serious threat to the captive mink and wild ... | 2010 | 20357945 |
| implementation and validation of a sensitive pcr detection method in the eradication campaign against aleutian mink disease virus. | aleutian mink disease virus (amdv) is a severe progressive disease causing multiple different clinical syndromes in mink. in denmark, the disease is notifiable and under official control. the control programme, based on serological screening, has confined successfully amdv to the northern part of denmark. however, re-infections and new introductions of virus into farms require a confirmatory virological test to verify the positive test results of single animals and ultimately to investigate dise ... | 2010 | 20951744 |
| diversity and stability of aleutian mink disease virus during bottleneck transitions resulting from eradication in domestic mink in denmark. | aleutian mink disease (plasmacytosis) virus (amdv) in domestic mink (neovison vison) has been subject to eradication in denmark since 1976. in 2001, approximately 5% of danish mink farms were still infected and all were located in the northern part of the peninsula of jutland. in the present study a total of 274 danish isolates of amdv collected during the two seasons of 2004 and 2005 were characterized by partial sequencing of the coding region of the non-structural (ns) proteins. older amdv is ... | 2010 | 21112164 |
| histopathologic and immunohistochemical lesions in liver of mink infected with aleutian disease virus. | parvovirus of aleutian disease causes mainly damage to kidneys, but immune complexes deposition and damage may occur also in other organs. in mink farms of latvia the liver dystrophy or hepatic lipidosis of mink is widely distributed. the goal of this study was to examine probability of liver damage and regeneration of mink infected with aleutian disease virus. liver injury was assessed histologically. the mink liver demonstrated inflammation of liver parenchyma and foci of fatty liver. in immun ... | 2011 | 21528714 |
| persistent spatial clusters of plasmacytosis among danish mink farms. | aleutian disease (plasmacytosis) is caused by the aleutian mink disease virus (amdv), an autonomous parvovirus and affects many mustelid species, including the american mink (neovisonvison). in denmark, an eradication program reduced the prevalence of test-positive farms from 100% in 1976 to 15% in 1996. nevertheless, the disease persists in the vendsyssel district of northern jutland, despite the eradication efforts. in this study, we used spatial epidemiological analysis to test for spatial au ... | 2011 | 21788091 |
| Novel amdovirus in gray foxes. | We used viral metagenomics to identify a novel parvovirus in tissues of a gray fox (Urocyon cinereoargenteus). Nearly full genome characterization and phylogenetic analyses showed this parvovirus (provisionally named gray fox amdovirus) to be distantly related to Aleutian mink disease virus, representing the second viral species in the Amdovirus genus. | 2011 | 22000359 |
| naturally occurring influenza a virus subtype h1n2 infection in a midwest united states mink (mustela vison) ranch. | influenza a virus (fluav) causes acute respiratory disease in humans and a variety of animal species. the virus tends to remain within the species of origin; nonetheless, naturally occurring cross-species transmission of fluav has been periodically documented. multiple cross-species transmissions of fluav have been reported from companion animals and captive wild animals, neither of which is historically considered as natural hosts of fluav. in the fall of 2010, mink (mustela vison) inhabiting a ... | 2011 | 22362526 |
| identification of biosecurity measures and spatial variables as potential risk factors for aleutian disease in danish mink farms. | eradication of aleutian disease was initiated in denmark in 1976. the prevalence of positive farms has since then been reduced from 100% to only being continuously present in the region of vendsyssel, northern denmark since 2004. in this study, we attempted to identify risk factors for the infection in this region based on logistic regression of spatial (environmental, neighbourhood) variables and biosecurity measures. information on potential biosecurity (management) risk factors in the region ... | 2012 | 22717327 |
| molecular epidemiology of aleutian disease virus in free-ranging domestic, hybrid, and wild mink. | aleutian mink disease (amd) is a prominent infectious disease in mink farms. the amd virus (amdv) has been well characterized in europe where american mink (neovison vison) are an introduced species; however, in north america, where american mink are native and the disease is thought to have originated, the virus' molecular epidemiology is unknown. as such, we characterized viral isolates from ontario free-ranging mink of domestic, hybrid, and wild origin at two proteins: ns1, a nonstructural pr ... | 2012 | 25568054 |
| prevalence of the aleutian mink disease virus infection in nova scotia, canada. | despite many years of testing mink for serum antibodies against the aleutian mink disease virus (amdv) by counterimmunoelectrophoresis (ciep) and elimination of reactors, this virus has remained the number one disease threat for the mink industry in nova scotia (ns). the objective of this study was to analyze ciep test results to determine the success of the amdv-control strategy in ns. a total of 2,964,920 ciep test results from 82 ranches, spanning an eight-year period between 1998 and 2005, w ... | 2012 | 22497690 |
| high prevalence of aleutian mink disease virus in free-ranging mink on a remote danish island. | aleutian mink disease virus (amdv) causes severe disease in farmed mink (neovison vison) worldwide. in denmark, amdv in farmed mink has been confined to the northern part of the mainland since 2002. from 1998 to 2009, samples from 396 free-ranging mink were collected from mainland denmark, and a low amdv antibody prevalence (3% of 296) was found using countercurrent immune electrophoresis. however, on the island of bornholm in the baltic sea, a high prevalence (45% of 142 mink) was detected in t ... | 2012 | 22493130 |
| phylogenetic analysis of the vp2 gene of aleutian mink disease parvoviruses isolated from 2009 to 2011 in china. | aleutian mink disease parvovirus (amdv) is a non-enveloped virus with a single-stranded dna genome that causes a fatal, usually persistent immune complex disease in minks. in this study, a total of 18,654 serum samples were collected from minks that were farmed in china from 2009 to 2011. after testing by counter-current immunoelectrophoresis (cie), the seroprevalence of amdv was found to be 68.67 %. the results show that there is a serious epidemic among chinese minks used for breeding. to gain ... | 2012 | 22415542 |
| genetic characterization of aleutian mink disease viruses isolated in china. | aleutian mink disease virus (amdv) is a parvovirus that causes an immune complex mediated disease in minks. to understand the genetic characterization of amdv in china, the genomic sequences of three isolates, adv-ln1, adv-ln2, and adv-ln3, from different farms in the northern china were analyzed. the results showed that the lengths of genomic sequences of three isolates were 4,543, 4,566, and 4,566 bp, respectively. they shared only 95.5-96.3 % nucleotide identity with each other. the nucleotid ... | 2012 | 22415541 |
| internal polyadenylation of parvoviral precursor mrna limits progeny virus production. | aleutian mink disease virus (amdv) is the only virus in the genus amdovirus of family parvoviridae. in adult mink, amdv causes a persistent infection associated with severe dysfunction of the immune system. cleavage of amdv capsid proteins has been previously shown to play a role in regulating progeny virus production (fang cheng et al., j. virol. 84:2687-2696, 2010). the present study shows that amdv has evolved a second strategy to limit expression of capsid proteins by preventing processing o ... | 2012 | 22361476 |
| evaluation of two enzyme-linked immunosorbent assays for serodiagnosis of aleutian mink disease virus infection in mink. | aleutian disease in mink is caused by infection with aleutian mink disease virus (amdv). in sweden, the infection most commonly causes classical aleutian disease in which the immune system fails to neutralize the virus and the infection becomes persistent. diagnosis of amdv infection is based on serological methods that detect virus-specific antibodies. traditionally counterimmunoelectrophoresis (ciep) has been the preferred method, but in order to enable automation interest has been paid to oth ... | 2013 | 24274663 |
| aleutian mink disease virus in furbearing mammals in nova scotia, canada. | aleutian mink disease virus (amdv) is widespread among ranched and free-ranging american mink in canada, but there is no information on its prevalence in other wild animal species. this paper describes the prevalence of amdv of 12 furbearing species in nova scotia (ns), canada. | 2013 | 23394546 |
| detection of mink enteritis virus by loop-mediated isothermal amplification (lamp). | loop-mediated isothermal amplification (lamp) method was discovered in the last decade but only used for the first time in the diagnosis of mink enteritis virus (mev) infection in this study. the amplification could be completed within 60 min, under isothermal condition at 65°c, by employing a set of four primers targeting the vp2 gene of mev. the lamp was more sensitive than the conventional pcr, with a detection limit of 10(-1) median tissue culture infective doses (tcid(50))/ml per reaction, ... | 2013 | 23183142 |
| [eenie, meenie, miney, moe, who is responsible for the antibody-dependent enhancement of aleutian mink disease parvovirus infection?]. | aleutian mink disease parvovirus (amdv) causes a persistent infection associated with immune complex disease, hypergammaglobulinemia, and high levels of antiviral antibodies. despite the presence of an antibody, the virus is not cleared in vivo. pre-existing antibodies may enhance viral infections, by fc-receptor-mediated antibody-dependent enhancement (ade), but the mechanism that underlies ade has not been fully defined. three models have been proposed, including: (1) interactions between anti ... | 2014 | 25272602 |
| application of real-time pcr to detect aleutian mink disease virus on environmental farm sources. | the aleutian mink virus (amdv) causes the aleutian mink disease (amd) or mink plasmacytosis, a disease responsible of high economic losses for industry worldwide. despite there is evidence of the environmental persistence of the virus, there is not literature on the detection of this virus in environmental samples in farms and this fact would have great importance in the control programs of the disease. in order to detect contamination caused by amdv on farms, several environmental samples were ... | 2014 | 25183237 |
| inactivation of aleutian mink disease virus through high temperature exposure in vitro and under field-based composting conditions. | disposal of manure contaminated with aleutian mink disease virus (amdv) is a significant concern to the mink industry. inactivation of amdv under field conditions has received limited attention in the scientific literature. we evaluated the thermal inactivation of amdv in vitro and during composting of mink manure. spleen homogenate containing amdv was heated under controlled conditions at 45°c, 55°c, and 65°c for 3 days. results of the in vitro study identified complete absence of viral replica ... | 2014 | 25139658 |