Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
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| molecular characterization of the spike gene of the porcine epidemic diarrhea virus in mexico, 2013-2016. | in mexico, the first outbreaks suggestive of the circulation of the porcine epidemic diarrhea virus (pedv) were identified at the beginning of july 2013. to identify the molecular characteristics of the pedv spike (s) gene in mexico, 116 samples of the intestine and diarrhea of piglets with clinical signs of porcine epidemic diarrhea (ped) were obtained. samples were collected from 14 farms located in six states of mexico (jalisco, puebla, sonora, veracruz, guanajuato, and michoacán) from 2013 t ... | 2017 | 29243063 |
| differential gene modulation of pattern-recognition receptor tlr and rig-i-like and downstream mediators on intestinal mucosa of pigs infected with pedv non s-indel and pedv s-indel strains. | porcine epidemic diarrhea virus (pedv) strains can be divided into non-s-indel and s-indel strains. pedv pathogenesis is strain-specific, and studies in neonatal pigs have demonstrated that the pedv non-s-indel strains are more pathogenic than the pedv s-indel strains. rna viruses, including pedv, can interact with a large number of pattern recognition receptors (prrs) in the intestinal mucosa, including toll-like receptors (tlrs) and rig-i-like receptors (rlrs). we investigated the differential ... | 2018 | 29249266 |
| construction and immunogenicity analysis of lactobacillus plantarum expressing a porcine epidemic diarrhea virus s gene fused to a dc-targeting peptide. | porcine epidemic diarrhea virus (pedv) is one of the most important causative pathogens of swine diarrhea, which is widely prevalent throughout the world and is responsible for significant economic losses in the commercial pig industry, both domestic and abroad. the spike (s) protein in the pedv capsid structure can carry the major b lymphocyte epitope, which induces production of neutralizing antibodies and provides immunoprotective effects. moreover, the conserved region encoded by the s gene ... | 2018 | 29288673 |
| a rapid method for antigen-specific hybridoma clone isolation. | using an enzyme-linked immunosorbent assay (elisa) and limited dilution methods to screen and clone antigen-specific hybridoma cells is extremely time-consuming and labor-intensive. this work features a simple and rapid cell surface fluorescence immunosorbent assay (csfia), designed for the detection and isolation of antigen-specific hybridoma clones. in this assay, antigens are first anchored to the hybridoma cell surface through a dual-functioning molecular oleyl-peg4000-nhs. specific antibodi ... | 2018 | 29290124 |
| mathematical modeling of porcine epidemic diarrhea virus dynamics within a farrow-to-finish swine farm to investigate the effects of control measures. | porcine epidemic diarrhea (ped) is a highly contagious enteric disease in swine that can cause devastating economic damage to pig producers. japan was severely affected by ped epidemics from 2013 through 2015, with over 1000 farms were affected during this period. although many studies have unraveled pathological and molecular characteristics of ped virus (pedv), the mechanism for within-farm spread is largely unknown. here, we constructed a deterministic compartmental model to quantitatively de ... | 2018 | 29290292 |
| identification of two antiviral inhibitors targeting 3c-like serine/3c-like protease of porcine reproductive and respiratory syndrome virus and porcine epidemic diarrhea virus. | porcine reproductive and respiratory syndrome virus (prrsv) and porcine epidemic diarrhea virus (pedv) are highly virulent and contagious porcine pathogens that cause tremendous economic losses to the swine industry worldwide. currently, there is no effective treatment for prrsv and pedv, and commercial vaccines do not induce sterilizing immunity. in this study, we screened a library of 1000 compounds and identified two specific inhibitors, designated compounds 2 and 3, which target the prrsv 3c ... | 2018 | 29291994 |
| vaccination of sows with a dendritic cell-targeted porcine epidemic diarrhea virus s1 protein-based candidate vaccine reduced viral shedding but exacerbated gross pathological lesions in suckling neonatal piglets. | porcine epidemic diarrhea virus (pedv) poses a serious threat to swine worldwide as evidenced by its recent introduction into the usa and the devastating economic impact it caused to the usa swine industry. commercial vaccines against pedv are available but their efficacies are inadequate. therefore, vaccines with improved efficacy are needed to effectively control pedv infections. we previously determined the immunogenicity of a novel dendritic cell (dc)-targeted pedv s1 protein-based subunit v ... | 2018 | 29300158 |
| dietary probiotic compound improves reproductive performance of porcine epidemic diarrhea virus-infected sows reared in a japanese commercial swine farm under vaccine control condition. | lactogenic immunity transferred to piglets after inoculation of a live vaccine to pregnant sows was proved limited to control porcine epidemic diarrhea (ped). hence, here we evaluated the efficacy of administration of a probiotic compound containingbacillus mesentericus, clostridium butyricum, andenterococcus faecalistogether with a commercial live-attenuated ped vaccine (nisseiken ped live vaccine, nisseiken, tokyo, japan) to improve the health and reproductive performance of ped-infected sows. ... | 2017 | 29312349 |
| real-time reverse transcription recombinase polymerase amplification assay for rapid detection of porcine epidemic diarrhea virus. | porcine epidemic diarrhea (ped), which is caused by porcine epidemic diarrhea virus (pedv), is an acute, highly contagious enteric disease characterized by severe watery diarrhea, vomiting, dehydration, and high mortality in suckling piglets. a real-time reverse-transcription recombinase polymerase amplification assay (rt-rpa) was developed based on the nucleocapsid gene of pedv. rt-rpa assay was performed at 40 °c for 20 min. the assay could detect both the classical and variant pedv strains, a ... | 2018 | 29330084 |
| glutathione-capped ag2s nanoclusters inhibit coronavirus proliferation through blockage of viral rna synthesis and budding. | development of novel antiviral reagents is of great importance for the control of virus spread. here, ag2s nanoclusters (ncs) were proved for the first time to possess highly efficient antiviral activity by using porcine epidemic diarrhea virus (pedv) as a model of coronavirus. analyses of virus titers showed that ag2s ncs significantly suppressed the infection of pedv by about 3 orders of magnitude at the noncytotoxic concentration at 12 h postinfection, which was further confirmed by the expre ... | 2018 | 29337529 |
| evidence of porcine epidemic diarrhea virus (pedv) shedding in semen from infected specific pathogen-free boars. | in 2013, ped emerged for the first time in the united states (us). the porcine epidemic diarrhea virus (pedv) spread quickly throughout north america. infection with pedv causes watery diarrhea and up to 100% mortality in piglets, particularly for highly pathogenic non-indel strains circulating in the us. pedv is mainly transmitted by the fecal-oral route. transmission via the venereal route has been suspected but not previously investigated. the aim of the study was to determine if pedv could b ... | 2018 | 29368629 |
| porcine epidemic diarrhea virus reduces feed efficiency in nursery pigs. | porcine epidemic diarrhea virus (pedv) infects enterocytes and in nursery pigs, results in diarrhea, anorexia, and reduced performance. therefore, the objective of this study was to determine how pedv infection influenced growth performance and repartitioning of amino acids and energy in nursery pigs. a total of 32 barrows and gilts, approximately 1 wk post-wean (bw = 8.46 ± 0.50 kg), and naïve for pedv were obtained, weighed, and allotted based on sex and bw to one of two treatments: 1) control ... | 2018 | 29378029 |
| tracking the origin and deciphering the phylogenetic relationship of porcine epidemic diarrhea virus in ecuador. | in 2010, new chinese strains of porcine epidemic diarrhea virus (pedv), clinically more severe than the classical strains, emerged. these strains were spread to united states in 2013 through an intercontinental transmission from china with further spreading across the world, evidencing the emergent nature of these strains. in the present study, an analysis of pedv field sequences from ecuador was conducted by comparing all the pedv s gene sequences available in the genbank database. phylogenetic ... | 2017 | 29379796 |
| porcine epidemic diarrhea virus-induced epidermal growth factor receptor activation impairs the antiviral activity of type i interferon. | porcine epidemic diarrhea virus (pedv) causes acute and devastating enteric disease in suckling piglets and results in huge economic losses in the pig industry worldwide. to establish productive infection, viruses must first circumvent the host innate immune response. in this study, we found that pedv infection stimulated epidermal growth factor receptor (egfr) activation, which has been linked to not only anticancer therapeutics, but also antiviral signaling. therefore, we determined whether eg ... | 2018 | 29386292 |
| efficacy of genogroup 1 based porcine epidemic diarrhea live vaccine against genogroup 2 field strain in japan. | porcine epidemic diarrhea (ped) is a lethal infectious disease in suckling piglets with symptoms including watery diarrhea caused by ped virus (pedv). since the late 1990's, live vaccines based on genogroup 1 virus have been used in japan, and a significant amount of the vaccine has been used even after new genogroups invaded in 2013. in this study, we evaluated the effect of a conventional ped live vaccine on a newly prevalent genogroup 2 field strain in experimental and field situations. | 2018 | 29394943 |
| efficacy of inactivated variant porcine epidemic diarrhea virus vaccines in growing pigs. | the first aim of this study was to develop a novel inactivated porcine epidemic diarrhea virus (pedv) vaccine using the recently isolated korean pedv qiap1401 strain and to evaluate its protective efficacy in growing pigs. the second was to determine the optimum adjuvant formulation of the inactivated pedv vaccine that induces protection against viral challenge. | 2018 | 29399581 |
| evaluation of a peroxygen-based disinfectant for inactivation of porcine epidemic diarrhea virus at low temperatures on metal surfaces. | porcine epidemic diarrhea virus (pedv) spread rapidly across the united states in part due to contaminated livestock trailers. the objective of this study was to test a peroxygen-based disinfectant for the ability to inactivate pedv on aluminum surfaces at 4 °c or -10 °c. forty 3-week-old individually housed barrows were used as a bioassay to determine the infectivity of pedv after treatment with either a 1:100 or 1:600 dilution of a peroxygen-based disinfectant with 10 or 30 min of contact time ... | 2018 | 29408040 |
| oral recombinant lactobacillus vaccine targeting the intestinal microfold cells and dendritic cells for delivering the core neutralizing epitope of porcine epidemic diarrhea virus. | porcine epidemic diarrhea caused by porcine epidemic diarrhea virus (pedv) has led to serious economic losses to the swine industry worldwide. in this study, an oral recombinant lactobacillus casei vaccine against pedv infection targeting the intestinal microfold (m) cells and dendritic cells (dcs) for delivering the core neutralizing epitope (coe) of pedv spike protein was developed with m cell-targeting peptide (col) and dendritic cell-targeting peptide (dcpep). the immunogenicity of the orall ... | 2018 | 29426335 |
| stability of porcine epidemic diarrhea virus on fomite materials at different temperatures. | indirect transmission of porcine epidemic diarrhea virus (pedv) ensues when susceptible animals contact pedv-contaminated fomite materials. although the survival of pedv under various phs and temperatures has been studied, virus stability on different fomite surfaces under varying temperature conditions has not been explored. hence, we evaluated the survival of pedv on inanimate objects routinely used on swine farms such as styrofoam, rubber, plastic, coveralls, and other equipment. the titer of ... | 2018 | 29438310 |
| complete genome sequence of a recombinant porcine epidemic diarrhea virus strain, ch/jxja/2017, isolated in jiangxi, china, in 2017. | the full-length genome sequence of a variant of porcine epidemic diarrhea virus (pedv), that of strain ch/jxja/2017, was highly homologous to ch/zmdzy/11, a highly virulent chinese pedv strain. ch/jxja/2017 had a distant relationship with the attenuated cv777 vaccine strain, but the insertion sites of the s1 gene were similar to those of the recombinant strain of ch/zmdzy/11. | 2018 | 29439052 |
| differences in the intestinal microbiota between uninfected piglets and piglets infected with porcine epidemic diarrhea virus. | porcine epidemic diarrhea, a disastrous gastrointestinal disease, causes great financial losses due to its high infectivity, morbidity and mortality in suckling piglets despite the development and application of various vaccines. in this study, high-throughput sequencing was used to explore differences in the intestinal microbiota between uninfected piglets and piglets infected with porcine epidemic diarrhea virus (pedv). the results revealed that the small intestinal microbiota of suckling pigl ... | 2018 | 29447243 |
| diarrhea caused by rotavirus a, b, and c in suckling piglets from southern brazil: molecular detection and histologic and immunohistochemical characterization. | rotavirus (rv) is an important viral pathogen causing diarrhea in piglets and other mammals worldwide. we describe 34 cases from 4 diarrheal outbreaks caused by rv in unvaccinated farrowing units in southern brazil from 2011 to 2013. we performed autopsy, histologic examinations, bacterial culture, rv immunohistochemistry (ihc), and enteric virus detection through molecular assays for rotavirus a, b, and c, transmissible gastroenteritis virus, porcine epidemic diarrhea virus, sapovirus, noroviru ... | 2018 | 29455625 |
| identification of porcine epidemic diarrhea virus variant with a large spike gene deletion from a clinical swine sample in the united states. | two genetically different porcine epidemic diarrhea virus (pedv) strains have been identified in the usa: us prototype (also called non-s indel) and s indel pedvs. in february 2017, a pedv variant (usa/ok10240-8/2017) was identified in a rectal swab from a sow farm in oklahoma, usa. complete genome sequence analyses indicated this pedv variant was genetically similar to us non-s indel strain but had a continuous 600-nt (200-aa) deletion in the n-terminal domain of the spike gene compared to non- ... | 2018 | 29468451 |
| metalloprotease adam17 regulates porcine epidemic diarrhea virus infection by modifying aminopeptidase n. | porcine epidemic diarrhea virus (pedv) is a causative agent of porcine epidemic diarrhea (ped). ped, characterized by acute diarrhea, vomiting, dehydration, has caused serious economic losses in pig industry worldwide. here, we demonstrate that activation of a disintergrin and metalloprotease 17 (adam17) induced the decrease of pedv infection in hek293 and ipec-j2 cells and the downregulation of cell surface aminopeptidase n (apn) expression, an important entry factor for pedv infection. further ... | 2018 | 29475600 |
| oral immunization against pedv with recombinant lactobacillus casei expressing dendritic cell-targeting peptide fusing coe protein of pedv in piglets. | porcine epidemic diarrhea (ped) is a highly contagious disease in newborn piglets. in our previous study, a genetically engineeredlactobacillus caseioral vaccine (ppg-coe-dcpep/l393) expressing a dendritic cell (dc)-targeting peptide fused with porcine epidemic diarrhea virus (pedv) coe antigen was developed. this vaccine induced significant levels of anti-pedv specific igg and iga antibody responses in mice, indicating a potential strategy against pedv infection. in this study,ppg-coe-dcpep/l39 ... | 2018 | 29494530 |
| development of a one-step rt-pcr assay for detection of pancoronaviruses (α-, β-, γ-, and δ-coronaviruses) using newly designed degenerate primers for porcine and avian `fecal samples. | coronaviruses (covs) are critical human and animal pathogens because of their potential to cause severe epidemics of respiratory or enteric diseases. in pigs, the newly emerged porcine deltacoronavirus (pdcov) and re-emerged porcine epidemic diarrhea virus (pedv) reported in the us and asia, as well as the discovery of novel covs in wild bats or birds, has necessitated development of improved detection and control measures for these covs. because the previous pancoronavirus (pancov) rt-pcr estab ... | 2018 | 29499225 |
| aminopeptidase-n-independent entry of porcine epidemic diarrhea virus into vero or porcine small intestine epithelial cells. | a monkey cell line vero (atcc ccl-81) is commonly used for porcine epidemic diarrhea virus (pedv) propagation in vitro. however, it is still controversial whether the porcine aminopeptidase n (papn) counterpart on vero cells (vero-apn) confers pedv entry. we found that endogenous expression of vero-apn was undetectable in the mrna and the protein levels in vero cells. we cloned the partial vero-apn gene (3340-bp) containing exons 1 to 9 from cellular dna and subsequently generated two apn-knocko ... | 2018 | 29502803 |
| complete genomic characterization of korean porcine epidemic diarrhea virus strain kupe21. | nationwide porcine epidemic diarrhea virus (pedv) outbreaks occurred in late 2013 in the republic of korea, resulting in an expansion of genomic data for korean pedvs. however, the data available for korean pedvs before 2013 are insufficient. therefore, we sequenced and analyzed the complete genome of a korean pedv strain, kupe21, which was isolated in the early 2000s. | 2018 | 29519828 |
| cellular hnrnp a1 interacts with nucleocapsid protein of porcine epidemic diarrhea virus and impairs viral replication. | the nucleocapsid (n) protein is a major structural component of porcine epidemic diarrhea virus (pedv), which is predicted to be a multifunctional protein in viral replication. heterogeneous nuclear ribonucleoprotein a1 (hnrnp a1) is a cellular protein participating in the splicing of pre-mrna in the nucleus and translation regulation in the cytoplasm. according to our previous proteomic study about pedv infection in vivo, hnrnp a1 was thought to be a cellular factor influencing pedv replication ... | 2018 | 29534017 |
| molecular characterization of us-like and asian non-s indel strains of porcine epidemic diarrhea virus (pedv) that circulated in japan during 2013-2016 and pedvs collected from recurrent outbreaks. | since late 2013, porcine epidemic diarrhea virus (pedv) has reemerged in japan and caused severe economic losses to the swine industry. although pedv vaccines have been used widely, the disease has swept rapidly across the county, and is commonly observed in ped-vaccinated farms, and has recurred in domestic herds. to better understand pedvs responsible for the reemerging outbreaks in japan, full-length spike (s), membrane (m), and nucleocapsid (n) genes of 45 pedvs collected in japan during 201 ... | 2018 | 29540176 |
| antiviral effect of lithium chloride on porcine epidemic diarrhea virus in vitro. | porcine epidemic diarrhea virus (pedv), a member of the coronaviridae family, causes acute diarrhea, vomiting, dehydration, and high mortality rates in neonatal piglets. severe outbreaks of pedv variants have re-emerged in asia and north america since 2010, causing tremendous economic losses to the swine industry. the lack of effective therapeutic treatment promotes the research for new antivirals. lithium chloride (licl) has been reported as a potential antiviral drug for certain viruses. in th ... | 2018 | 29547727 |
| epidemiology and phylogeny of spike gene of porcine epidemic diarrhea virus from yunnan, china. | porcine epidemic diarrhea (ped) causes acute enteric disease and yellowish watery diarrhea, making piglets fast dehydration to death. ped threatens pig industry and leads to substantial economic losses. after the first reports, ped in yunnan province, china was again identified in 2013 during an epidemiological survey, with follow-up data showing an overall positive rate of 17.47% during 2013-2017, lower than that in other provinces in china. the complete s gene of porcine epidemic diarrhea viru ... | 2018 | 29548744 |
| assessment of reproductive performance in f1sows exposed to the porcine epidemic diarrhea virus at different periods of production stage on farms with different hygienic environments. | porcine epidemic diarrhea (ped) occurred in japan in 2013 after an interval of 7 years. the present study assessed individual productivity of sows exposed to ped virus at different periods of the production stage. the present study was performed at three commercial farms that had ped outbreak during the month of december 2013. herd immunization was conducted for all gilts and sows. the production records were obtained for sows that were alive during the ped outbreak at each farm. the sows were c ... | 2018 | 29567203 |
| development of a rapid immunochromatographic assay for detection of antibodies against porcine epidemic diarrhea virus. | an immunochromatographic test strip was developed for the detection of antibodies against porcine epidemic diarrhea virus (pedv) in porcine serum. in this test, the recombinant nucleocapsid protein of pedv was expressed in soluble form and purified by using ni-nta resin and gel filtration chromatography. the purified recombinant n protein conjugated with colloidal gold was dispensed on a conjugate pad as the detector. staphylococcal protein a and rabbit anti-n protein igg were blotted on a nitro ... | 2018 | 29624018 |
| infection, genetic and virulence characteristics of porcine epidemic diarrhea virus in northwest china. | from september 2015 to may 2016, epidemic outbreaks of a diarrheal disease caused severe economic losses to the swine industry in northwest china. typical clinical symptoms of the disease included severe diarrhea, vomiting, dehydration and death. in order to identify the pathogen, 27 intestinal samples were collected from dead piglets in shaanxi, gansu and qinghai provinces and from ningxia hui autonomous region. all samples were tested using rt-pcr to detect rotavirus (rv), porcine epidemic dia ... | 2018 | 29625238 |
| role of animal movement and indirect contact among farms in transmission of porcine epidemic diarrhea virus. | epidemiological models of the spread of pathogens in livestock populations primarily focus on direct contact between farms based on animal movement data, and in some cases, local spatial spread based on proximity between premises. the roles of other types of indirect contact among farms is rarely accounted for. in addition, data on animal movements is seldom available in the united states. however, the spread of porcine epidemic diarrhea virus (pedv) in u.s. swine represents one of the best docu ... | 2018 | 29673815 |
| the s gene is necessary but not sufficient for the virulence of porcine epidemic diarrhea virus novel variant strain bj2011c. | the recently emerged highly virulent variants of porcine epidemic diarrhea virus (pedv) have caused colossal economic losses to the worldwide swine industry. in this study, we investigated the viral virulence determinants by constructing a series of chimeric mutants between the highly virulent strain bj2011c and the avirulent strain chm2013. when tested in the 2-day-old piglet model, wild-type (wt) bj2011c caused severe diarrhea and death of the piglets within 72 h. in contrast, its chimeric der ... | 2018 | 29695430 |
| passive immunity to porcine epidemic diarrhea virus following immunization of pregnant gilts with a recombinant orf virus vector expressing the spike protein. | passive immunity is critical for protection of neonatal piglets against porcine epidemic diarrhea virus (pedv). here, we investigated the immunogenicity of an orf virus (orfv) vector expressing the full-length spike (s) protein of pedv (orfv-pedv-s) in pregnant gilts and its ability to confer passive immunity and protection in piglets. three doses of orfv-pedv-s were given to two groups of pedv-negative pregnant gilts, with the last dose being administered two weeks prior to farrowing. one of th ... | 2018 | 29725899 |
| porcine rotavirus c in pigs with gastroenteritis on thai swine farms, 2011-2016. | swine are economically important food animals, but highly contagious porcine epidemic diarrhea virus (pedv) and rotavirus can afflict pig herds and contribute significantly to piglet morbidity and mortality. while there have been studies on rotavirus group a (rva) in thailand, reports of rotavirus group c (rvc) are limited. here, we aimed to identify the prevalence of rvc circulating on thai commercial swine farms. we analyzed 769 feces and intestine mucosal contents of pigs affected with diarrh ... | 2018 | 29761045 |
| new variants of porcine epidemic diarrhea virus with large deletions in the spike protein, identified in the united states, 2016-2017. | four types of porcine epidemic diarrhea virus (pedv) variants with a large deletion in the spike protein were detected, together with the original us pedv, from pig fecal and oral fluid samples collected during 2016-2017 in the us. two of the variants are similar to those identified in japan: one contains a 194-aa deletion, the same as pedv variant ttr-2/jpn/2014, while the other contains a 204-aa deletion, the same as pedv variant jka-292/cs1de204. two new s1 ntd-del pedv variants were found: o ... | 2018 | 29789941 |
| porcine epidemic diarrhea virus through p53-dependent pathway causes cell cycle arrest in the g0/g1 phase. | porcine epidemic diarrhea virus (pedv), an enteropathogenic alphacoronavirus, has caused enormous economic losses in the swine industry. p53 protein exists in a wide variety of animal cells, which is involved in cell cycle regulation, apoptosis, cell differentiation and other biological functions. in this study, we investigated the effects of pedv infection on the cell cycle of vero cells and p53 activation. the results demonstrated that pedv infection induces cell cycle arrest at g0/g1 phase in ... | 2018 | 29800601 |
| metagenomic analysis of the rna fraction of the fecal virome indicates high diversity in pigs infected by porcine endemic diarrhea virus in the united states. | emergence and re-emergence of porcine epidemic diarrhea virus (pedv) in north america, asia and europe has caused severe economic loss to the global swine industry. however, the virome of pedv infected pigs and its effect on disease severity remains unknown. the advancements of sequencing technology have made it possible to characterize the entire microbiome of different body sites for any host. | 2018 | 29801460 |
| characterization of the interaction between recombinant porcine aminopeptidase n and spike glycoprotein of porcine epidemic diarrhea virus. | porcine epidemic diarrhea (ped) has caused huge economic losses to the global pork industry. infection by its causative agent ped virus (pedv), an alpha-coronavirus, was previously proven to be mediated by its spike (s) glycoprotein and a cellular receptor porcine aminopeptidase n (papn). interestingly, some recent studies have indicated that papn is not a functional receptor for pedv. to date, there is a lack of a direct evidence for the interaction between papn and pedv s protein in vitro. her ... | 2018 | 29802920 |
| probiotic supplementation improves reproductive performance of unvaccinated farmed sows infected with porcine epidemic diarrhea virus. | we investigated if probiotic supplementation could improve the health and reproductive performance of unvaccinated lactating sows infected with porcine epidemic diarrhea (ped) virus. twenty unvaccinated pregnant sows were equally allocated to probiotic-supplemented (p) and control (c) groups. for the experiment, 15 g/day of probiotic compound bio-three pz was given to p sows. reproductive performance was checked daily. the number of neonates fostered by each sow was maintained at eight throughou ... | 2018 | 29806133 |
| insights into recombination-like events leading to outbreaks in usa through a retrospective study of porcine epidemic diarrhea virus isolates from china. | in april 2013, severe porcine epidemic diarrhea (ped) outbreaks first appeared in usa, causing significant financial losses. to detect the geographic origin and timing of usa porcine epidemic diarrhea virus (pedv) isolates, we conducted a retrospective study to isolate 19 pedv strains from positive samples obtained prior to april 2013. the results of phylogenetic and recombination analyses showed that gds10 strain shared a common ancestor with co13 strain, which was obtained from a piglet with s ... | 2018 | 29879479 |
| development of sandwich enzyme-linked immunosorbent assay for the detection of porcine epidemic diarrhea virus in fecal samples. | porcine epidemic diarrhea virus (pedv) causes acute diarrhea and dehydration in new-born piglets with subsequent economic losses to swine industry. in the current study, gene encoding of 381aa-792aa spike protein (s1) with the main epitope relative to virus neutralization of pedv was amplified by rt-pcr and inserted into vector pet-30a(+). the plasmid was transferred into escherichia coli bl21 (de3). meanwhile, recombinant protein expression was induced by isopropy1-β-galactopyranoside (iptg). a ... | 2018 | 29894809 |
| immunohistochemical detection of the vomiting-inducing monoamine neurotransmitter serotonin and enterochromaffin cells in the intestines of conventional or gnotobiotic (gn) pigs infected with porcine epidemic diarrhea virus (pedv) and serum cytokine responses of gn pigs to acute pedv infection. | serotonin is a critical monoamine neurotransmitter molecule stored and released from enterochromaffin (ec) cells into the gut submucosa, transmitting the vomiting signal to the brain. we studied one mechanism by which vomiting is induced in pigs infected with porcine epidemic diarrhea virus (pedv) by characterization of swine ec cells by immunohistochemistry. conventional or gnotobiotic (gn) 9-day-old pigs [pedv-inoculated (n = 12); mock (n = 14)] were inoculated orally (8.9-9.2 log10 genomic eq ... | 2018 | 29909130 |
| spillover of swine coronaviruses, united states. | porcine epidemic diarrhea virus, a pathogen first detected in us domestic swine in 2013, has rapidly spilled over into feral swine populations. a better understanding of the factors associated with pathogen emergence is needed to better manage, and ultimately prevent, future spillover events from domestic to nondomestic animals. | 2018 | 29912697 |
| epitope selection and their placement for increased virus neutralization in a novel vaccination strategy for porcine epidemic diarrhea virus utilizing the hepatitis b virus core antigen. | porcine epidemic diarrhea virus (pedv) is a member of the alphacoronaviridae genus within the coronaviridae family. it is the causative agent of porcine epidemic diarrhea, a disease that can have mortality rates as high as 100% in suckling piglets. pedv causes severe economic loss, and has been in existence for decades. a panzootic starting in 2010 renewed interest in the development of a universal vaccine toward pedv. this report details several design changes made to a hepatitis b virus core a ... | 2018 | 29914846 |
| dimerization of coronavirus nsp9 with diverse modes enhances its nucleic acid binding affinity. | coronaviruses pose serious health threats to humans and other animals. understanding the mechanisms of their replication has important implications for global health and economic stability. nonstructural protein 9 (nsp9) is an essential rna binding protein for coronavirus replication. however, the mechanisms of the dimerization and nucleic acid binding of nsp9 remain elusive. here, we report four crystal structures, including wild-type porcine delta coronavirus (pdcov) nsp9, pdcov nsp9-δn7 (n-te ... | 2018 | 29925659 |
| development of a real time reverse transcription loop-mediated isothermal amplification method (rt-lamp) for detection of a novel swine acute diarrhea syndrome coronavirus (sads-cov). | a novel swine acute diarrhea syndrome coronavirus (sads-cov) that causes severe diarrhea in suckling piglets was identified in southern china in 2017. a simple and rapid detection test was developed for this virus using real-time rt-lamp based on the conserved n gene of the virus. the method had a detection limit of 1.0 × 101 copies/μl with no cross-reactions with classical swine fever virus, porcine and respiratory syndrome virus na, porcine and respiratory syndrome virus eu, transmissible gast ... | 2018 | 29936095 |
| construction and characterization of thymidine auxotrophic (δthya) recombinant lactobacillus casei expressing bovine lactoferricin. | lactobacillus casei (l. casei) is well known for its probiotic property in human and animals. lactoferricin (lfcin) polypeptide can effectively modulate host immune responses and have antimicrobial activity in vivo and in vitro. in order to develop a food-grade l. casei system constitutively expressing bovine lfcin, this study constructed a thymidine auxotrophy (δthya) recombinant l. casei. | 2018 | 29945678 |
| porcine epidemic diarrhea virus and porcine deltacoronavirus not detected in waterfowl in the north american mississippi migratory bird flyway in 2013. | cloacal swab samples collected from 538 migratory waterfowl along the mississippi migratory bird flyway in 2013 were tested for porcine epidemic diarrhea virus and porcine deltacoronavirus. neither virus was detected in any of the samples, indicating that waterfowl likely did not contribute to the rapid spread of these viruses within central us. | 2019 | 29953312 |
| display of porcine epidemic diarrhea virus spike protein on baculovirus to improve immunogenicity and protective efficacy. | a new variant of the porcine epidemic diarrhea virus (pedv) is an emerging swine disease, killing considerable numbers of neonatal piglets in north america and asia in recent years. to generate immunogens mimicking the complex spike (s) protein folding with proper posttranslational modification to mount a robust immune response against the highly virulent pedv, two baculoviruses displaying the full-length s protein (s-bac) and the s1 protein (s1-bac) of the virulent taiwan genotype 2b (g2b) pedv ... | 2018 | 29954081 |
| nanopore sequencing as a revolutionary diagnostic tool for porcine viral enteric disease complexes identifies porcine kobuvirus as an important enteric virus. | enteric diseases in swine are often caused by different pathogens and thus metagenomics are a useful tool for diagnostics. the capacities of nanopore sequencing for viral diagnostics were investigated here. first, cell culture-grown porcine epidemic diarrhea virus and rotavirus a were pooled and sequenced on a minion. reads were already detected at 7 seconds after start of sequencing, resulting in high sequencing depths (19.2 to 103.5x) after 3 h. next, diarrheic feces of a one-week-old piglet w ... | 2018 | 29959349 |
| efficacy of heat-labile enterotoxin b subunit-adjuvanted parenteral porcine epidemic diarrhea virus trimeric spike subunit vaccine in piglets. | devastating outbreaks of porcine epidemic diarrhea (ped) started in china in late 2010 and rapidly spread to north america and asia causing severe diarrhea and high mortality in neonatal piglets, indicating that a new generation of vaccine against porcine epidemic diarrhea virus (pedv) is urgently needed. in the present study, to mimic the native spike (s) glycoprotein, a stable cell line producing the trimeric ectodomain of s glycoprotein of the pedv pintung-52 (pedv-pt) strain was successfully ... | 2018 | 29961099 |
| nucleocapsid protein from porcine epidemic diarrhea virus isolates can antagonize interferon-λ production by blocking the nuclear factor-κb nuclear translocation. | porcine epidemic diarrhea virus (pedv) is a highly infectious pathogen that can cause severe diseases in pigs and result in enormous economic losses in the worldwide swine industry. previous studies revealed that pedv exhibits an obvious capacity for modulating interferon (ifn) signaling or expression. the newly discovered type iii ifn, which plays a crucial role in antiviral immunity, has strong antiviral activity against pedv proliferation in ipec-j2 cells. in this study, we aimed to investiga ... | 2018 | 29971995 |
| porcine epidemic diarrhea virus (pedv) introduction into a naive dutch pig population in 2014. | porcine epidemic diarrhea virus (pedv) is the highly contagious, causative agent of an economically important acute enteric disease in pigs of all ages. the disease is characterized by diarrhea and dehydration causing mortality and growth retardation. in the last few decades, only classical pedv was reported sporadically in europe, but in 2014 outbreaks of pedv were described in germany. phylogenetic analysis showed a very high nucleotide similarity with a variant of pedv that was isolated in th ... | 2018 | 29981699 |
| porcine epidemic diarrhea vaccine evaluation using a newly isolated strain from korea. | porcine epidemic diarrhea virus (pedv) infects pigs and causes an enteric disease that is characterized by vomiting and watery diarrhea. pedv outbreaks have a tremendous financial impact on the worldwide pork industry. in south korea, the incidence of pedv has continued despite nationwide use of attenuated and inactivated vaccines, raising questions regarding the current vaccines' efficacy and the need for new vaccine development. in the present study, we isolated a new korean pedv epidemic stra ... | 2018 | 29981703 |
| isolation and characterization of chinese porcine epidemic diarrhea virus with novel mutations and deletions in the s gene. | porcine epidemic diarrhea (pedv) has raised growing concerns in the pig-breeding industry because it has caused significant economic losses. to better understand the molecular epidemiology and genetic diversity of pedv field isolates, in this study, the complete spike (s) and orf3 genes of 17 pedv variants in zhejiang, china during 2014 to 2017, were characterized and analyzed. phylogenetic analysis based on the s gene and orf3 gene of these 17 novel pedv strains and pedv reference strains indic ... | 2018 | 29981713 |
| n-terminal domain of the spike protein of porcine epidemic diarrhea virus as a new candidate molecule for a mucosal vaccine. | porcine epidemic diarrhea virus (pedv) is a contagious coronavirus infecting pigs that leads to significant economic losses in the swine industry. given that pedv infection occurs in gut epithelial cells mainly via the fecal-oral route, induction of pedv-specific immune responses in the mucosal compartment is required for protective immunity against viral infection. however, an effective mucosal vaccine against the currently prevalent pedv strain is not available. in this study, we demonstrated ... | 2018 | 29984039 |
| comparative pharmacokinetics and preliminary pharmacodynamics evaluation of piscidin 1 against prv and pedv in rats. | antimicrobial peptide (piscidin-1) is an effective natural polypeptide, which has great influence and potential on porcine epidemic diarrhea virus (pedv) and pseudorabies virus (prv). as an alternative antibiotic substitute, piscidin-1 was subjected for pharmacokinetics study with three administration routes (i.v, i.m, and p.o) after a single dose of 2 mg/kg in rats and preliminary pharmacodynamics including antiviral activity in cell against pedv and prv. based on 50 percent tissue culture infe ... | 2018 | 29988520 |
| [microrna expression profiles of porcine kidney 15 cell line infected with porcine epidemic diahorrea virus]. | to explore the effect of porcine epidemic diarrhea virus(pedv)on microrna expression profiles of porcine kidney 15cell(pk-15),total rna was isolated from pk-15 cells with or without pedv infection. then, we obtained the mirnas by using solexa sequencing technology and analyzed these differentially expressed mirnas. heatmap cluster analysis and go (ontology, go) (gene function, mf, molecular) analysis was performed on the significant differences in expression of the mirna, and 10 significant diff ... | 2016 | 29995369 |
| [identification and characterzation of the porcine epidemic diarrhea virus (pedv) isolates]. | to explore the porcine epidemic diarrhea virus(pedv)epidemiology in domestic pigs, virus isolation and identification were carried out in pig herds in shanghai and jiangsu province. based on sequences of pedv strains in genbank, a pair of primers targeting the m gene were designed for pedv detection. two clinical pig diarrhea samples were amplified and sequencing results confirmed that the fragments amplified were m gene. pedv-positive samples were inoculated in vero cells with different concent ... | 2016 | 30001583 |
| [development and application of reverse transcription loop-mediated isothermal amplification method for detection of porcine rotavirus of swine]. | we wished to establish a method for rapid and sensitive detection of reverse transcription loop-mediated isothermal amplification(rt-lamp)for the rapid and sensitive detection of porcine rotavirus (porv). according to the published porv vp7 sequences in genbank,6specific primers were designed. according to the concentrations of foward and reverse primers, bst dna polymerase, mg(2+), and dntp, reaction conditions were optimized. results revealed the concentration ratio of foward and reverse prime ... | 2016 | 30004206 |
| time-calibrated phylogenomics of the porcine epidemic diarrhea virus: genome-wide insights into the spatio-temporal dynamics. | porcine epidemic diarrhea virus (pedv), the causative agent of porcine epidemic diarrhea (ped), has led to tremendous economic losses in the global swine industry. although the phylogeny of pedv has been investigated extensively at the molecular level, there was no time-calibrated phylogenomic study on the virus. to improve insight into this topic, we analyzed 138 published genome sequences using the bayesian coalescent analyses as well as bayesian inferences and maximum likelihood methods. all ... | 2018 | 30047110 |
| detection, sequence analysis, and antibody prevalence of porcine deltacoronavirus in taiwan. | porcine deltacoronavirus (pdcov) was initially documented in hong kong and later in the united states, south korea, and thailand. to investigate if pdcov is also present in taiwan, three swine coronaviruses-pdcov, porcine epidemic diarrhea virus (pedv), and transmissible gastroenteritis coronavirus (tgev)-were tested using real-time reverse transcription polymerase chain reaction (rrt-pcr) in 172 rectal swab samples from piglets exhibiting diarrhea between january 2016 and may 2017 on 68 pig far ... | 2018 | 30051342 |
| evaluation of the effects of flushing feed manufacturing equipment with chemically treated rice hulls on porcine epidemic diarrhea virus cross-contamination during feed manufacturing. | various strategies have been proposed to mitigate potential risk of porcine epidemic diarrhea virus (pedv) transmission via feed and feed ingredients. wet disinfection has been found to be the most effective decontamination of feed mill surfaces; however, this is not practical on a commercial feed production scale. another potential mitigation strategy would be using chemically treated rice hulls flushed through the feed manufacturing equipment. therefore, the objective of this study was to dete ... | 2018 | 30052979 |
| the accessory protein orf3 contributes to porcine epidemic diarrhea virus replication by direct binding to the spike protein. | the porcine epidemic diarrhea virus (pedv) is an important swine pathogen responsible for severe watery diarrhea, particularly in neonatal piglets. despite extensive studies performed to elucidate the function of several viral proteins, the contribution of an accessory protein orf3 in pedv replication is still largely unknown. here, we constructed expression plasmids as well as recombinant pedv carrying myc-tagged orf3 to assess their expression and subcellular localization in both transfected a ... | 2018 | 30060558 |
| preparation and characterization of an attenuated porcine epidemic diarrhea virus strain by serial passaging. | porcine epidemic diarrhea virus (pedv) is prevalent in most parts of the world. owing to its antigenic variation, prevention of the diseases caused by this virus is difficult. in this study, two pedv isolates with similar growth kinetics were successfully propagated in vero cells. complete genome sequence analysis showed that they have a 49nt deletion in the orf3 gene and were classified into group 1, the same group that includes the classical cv777 strain. recombination analysis revealed that t ... | 2018 | 30062639 |
| surfactin inhibits membrane fusion during invasion of epithelial cells by enveloped viruses. | because membrane fusion is a crucial step in the process by which enveloped viruses invade host cells, membrane fusion inhibitors can be effective drugs against enveloped viruses. we found that surfactin from bacillus subtilis can suppress the proliferation of porcine epidemic diarrhea virus (pedv) and transmissible gastroenteritis virus (tgev) in epithelial cells at a relatively low concentration range (15 to 50 μg/ml), without cytotoxicity or viral membrane disruption. membrane fusion inhibiti ... | 2018 | 30068648 |
| different intestinal tropism of the g2b taiwan porcine epidemic diarrhea virus-pintung 52 strain in conventional 7-day-old piglets. | the group 2b (g2b) porcine epidemic diarrhea virus (pedv) that emerged in 2013 has since caused devastating diseases and economic loss. the full-length genome of the g2b taiwan pedv-pintung 52 (pedv-pt) strain and its intestinal tropism by evaluating the pathological changes in the original pedv-pt infected field piglet and orally inoculation of either 10, 103, or 105 50% tissue culture infective dose/ml (tcid50/ml) of the plaque-purified pedv-pt-passage 5 (p5) in 7-day-old conventional piglets ... | 2018 | 30089548 |
| feed batch sequencing to decrease the risk of porcine epidemic diarrhea virus (pedv) cross-contamination during feed manufacturing. | feed has been identified as a vector of transmission for porcine epidemic diarrhea virus (pedv). the objective of this study was to determine if feed batch sequencing methods could minimize pedv cross-contamination. porcine epidemic diarrhea virus-free swine feed was manufactured to represent the negative control. a 50 kg feed batch was mixed in a pilot scale feed mill for 5 min, sampled, then discharged for 10 min into a bucket elevator and sampled again upon exit. next, a pathogenic pedv isola ... | 2018 | 30099515 |
| evolutionary and genotypic analyses of global porcine epidemic diarrhea virus strains. | porcine epidemic diarrhea virus (pedv), which re-emerged in china in october 2010, has spread rapidly worldwide. detailed analyses of the complete genomes of different pedv strains are essential to understand the relationships among re-emerging and historic strains worldwide. here, we analysed the complete genomes of 409 strains from different countries, which were classified into five subgroup strains (i.e., gi-a, gi-b, gii-a, gii-b, and gii-c). phylogenetic study of different genes in the pedv ... | 2019 | 30102851 |
| oral administration of inactivated porcine epidemic diarrhea virus activate dcs in porcine peyer's patches. | peyer's patches (pps) can be considered as the immune site of the intestine. within pps, dendritic cells (dcs) can uptake antigens from the gut lumen by extending dendrites into epithelium, and process it and then present to lymphocytes, which effectively antigen produces an immune response. porcine epidemic diarrhea virus (pedv) is the causative agent of porcine epidemic diarrhea (ped), an acute and highly contagious enteric viral disease. the interaction between inactivated porcine epidemic di ... | 2018 | 30115049 |
| development and application of an indirect elisa for the detection of antibodies to porcine epidemic diarrhea virus based on a recombinant spike protein. | as the major causative agent of swine viral diarrhea, porcine epidemic diarrhea virus (pedv) has caused massive losses to the economies of swine raising countries. accordingly, the serological detection of corresponding antibodies would be beneficial to diagnose pedv indirectly to control the disease. in this study, an indirect enzyme-linked immunosorbent assay (elisa) based on the recombinant truncated spike (s) protein of pedv was developed and validated. | 2018 | 30126390 |
| characterization of influenza a virus pseudotyped with the spike protein of porcine epidemic diarrhea virus. | the coronavirus spike protein and the influenza virus hemagglutinin are class i viral membrane fusion proteins. while the two proteins display strong structural conservation and the mechanisms underlying membrane fusion are similar, they share no sequence similarity. whether they are functionally interchangeable is currently unknown. in this study, we constructed sciav-s, a single-cycle influenza a virus pseudotyped with the spike protein of porcine epidemic diarrhea virus (pedv), and demonstrat ... | 2018 | 30136251 |
| porcine epidemic diarrhea virus papain-like protease 2 can be noncompetitively inhibited by 6-thioguanine. | porcine epidemic diarrhea virus (pedv) is a coronavirus (cov) discovered in the 1970s that infects the intestinal tract of pigs, resulting in diarrhea and vomiting. it can cause extreme dehydration and death in neonatal piglets. in asia, modified live attenuated vaccines have been used to control pedv infection in recent years. however, a new strain of pedv that belongs to genogroup 2a appeared in the usa in 2013 and then quickly spread to canada and mexico as well as asian and european countrie ... | 2018 | 30138642 |
| single-domain antibodies as promising experimental tools in imaging and isolation of porcine epidemic diarrhea virus. | single-domain antibody (sdab) or nanobody possesses specific features non-accessible for conventional antibodies that make them suitable for research and biotechnological applications. porcine epidemic diarrhea virus (pedv) causes lethal diarrhea in piglets, resulting in great economic losses all over the world. to detect and isolate pedv rapidly and accurately is important for the control and further research of the clinical pedv strains. in this study, four sdab fragments (sdab-mc19/29/30/37) ... | 2018 | 30143837 |
| characterisation of porcine epidemic diarrhea virus isolates during the 2014-2015 outbreak in the philippines. | the viral agent of the porcine epidemic diarrhea (ped) was investigated during the reported 2014-2015 outbreaks in commercial farms in central luzon, philippines. the study covered detection of ped virus (pedv) in fecal and intestinal samples through reverse transcription pcr and sequence analysis of the nucleocapsid (n) gene. results showed that 10 out of 34 fecal and intestinal samples examined were positive for pedv. the partial nucleotide sequence of the n gene of the field samples showed 98 ... | 2018 | 30159369 |
| neutralizing antibodies against porcine epidemic diarrhea virus block virus attachment and internalization. | porcine epidemic diarrhea virus (pedv) is emerging as a pathogenic coronavirus that causes a huge economic burden to the swine industry. interaction of the viral spike (s) surface glycoprotein with the host cell receptor is recognized as the first step of infection and is the main determinant of virus tropism. the mechanisms by which neutralizing antibodies inhibit pedv have not been defined. isolating pedv neutralizing antibodies are crucial to identifying the receptor-binding domains of the vi ... | 2018 | 30165871 |
| three main inducers of alphacoronavirus infection of enterocytes: sialic acid, proteases, and low ph. | transmissible gastroenteritis virus (tgev) and porcine epidemic diarrhea virus (pedv) are similar coronaviruses, causing diseases characterized by vomiting, diarrhea, and death from severe dehydration in piglets. thus, they have caused huge losses to the swine-breeding industry worldwide. nowadays, they are easily transmitted among the continents via vehicles, equipment, and cargo. both viruses establish an infection in porcine enterocytes in the small intestine, and their spike (s) proteins pla ... | 2018 | 30176660 |
| porcine epidemic diarrhea: an emerging disease in tibetan pigs in tibet, china. | porcine epidemic diarrhea virus (pedv) is a devastating cause of diarrhea in pigs worldwide, and has caused enormous economic losses in the pork industry. to date, however, it is still unclear whether tibetan pigs from the tibetan plateau of china are exposed to ped. this study was to investigate the seroprevalence of ped in tibetan pigs in tibet, china. a total of 364 serum samples were collected from tibetan pigs in nyingchi in 2014 and 2015. each sample was assayed for ped antibodies by comme ... | 2019 | 30191422 |
| s1 subunit of spike protein from a current highly virulent porcine epidemic diarrhea virus is an important determinant of virulence in piglets. | base on the sequence of s genes, which encode spike proteins, we previously identified three different types (north american, s indel, and s large-del types) of porcine epidemic diarrhea virus (pedv) that have re-emerged in japan since 2013. based on experimental infections with the north american and s large-del types, we also hypothesized that pedv virulence may be linked to the s1 subunit of the s protein. to test this hypothesis, we have now assayed in gnotobiotic piglets various recombinant ... | 2018 | 30200258 |
| the effect of chemical clarification of oral fluids on porcine epidemic diarrhea virus antibody responses. | routine testing of breeding herd oral fluid (of) samples for porcine epidemic diarrhea virus (pedv) igg and/or iga is used to track levels of pedv immunity over time. however, ofs contain particles of feed, feces, and inorganic material that detract from the quality of the sample. we clarified swine of samples using lyophilized chitosan-based formulas (a-c) tested by pedv igg and iga elisas. to evaluate both the immediate and residual effects of treatment on antibody detection, samples were test ... | 2018 | 30204059 |
| a convenient colorimetric assay for the quantification of porcine epidemic diarrhea virus and neutralizing antibodies. | neonatal enteritis caused by the porcine epidemic diarrhea virus (pedv) is an important cause of high mortality and economic losses to the swine industry. virus neutralization (v/n) assays are commonly requested in diagnostic laboratories for the assessment of protective antibodies. however, the visual assessment of viral cytopathic effects by operators to determine antibody titers or for viral quantification is a tedious, subjective and time-consuming process, especially when high volume testin ... | 2018 | 30218684 |
| the immunological functions of muramyl dipeptide compound adjuvant on humoral, cellular-mediated and mucosal immune responses to pedv inactivated vaccine in mice. | the muramyl dipeptide compound adjuvant, cvc1303, was one new resigned adjuvant to pedv inactivated vaccine. exploring the effects of cvc1303 on the immune induction to pedv vaccine was of vital importance to the clinical application. | 2018 | 30227812 |
| caerin1.1 suppresses the growth of porcine epidemic diarrhea virus in vitro via direct binding to the virus. | porcine epidemic diarrhea (ped) has re-emerged in recent years and has already caused huge economic losses to the porcine industry all over the world. therefore, it is urgent for us to find out efficient ways to prevent and control this disease. in this study, the antiviral activity of a cationic amphibian antimicrobial peptide caerin1.1 against porcine epidemic diarrhea virus (pedv) was evaluated by an in vitro system using vero cells. we found that even at a very low concentration, caerin1.1 h ... | 2018 | 30231560 |
| an alternative pathway of enteric pedv dissemination from nasal cavity to intestinal mucosa in swine. | porcine epidemic diarrhea virus (pedv) has catastrophic impacts on the global pig industry. although the fecal-oral route is generally accepted, an increased number of reports indicate that airborne transmission may contribute to pedv outbreak. here, we show that pedv could cause typical diarrhea in piglets through a nasal spray. firstly, pedv can develop a transient nasal epithelium infection. subsequently, pedv-carrying dendritic cells (dcs) allow the virus to be transferred to cd3+ t cells vi ... | 2018 | 30232333 |
| oral administration of coated pedv-loaded microspheres elicited pedv-specific immunity in weaned piglets. | porcine epidemic diarrhea virus (pedv) infects pigs of all ages by invading villous epithelial cells of the small intestine causing severe diarrhea with high mortality rate in suckling piglets. mucosal immunity is believed to play an important role in pedv control and mucosal delivery of vaccines induces mucosal immunity more efficiently than parenteral vaccination. in this study, coated pedv-loaded microspheres with the size range of 700-900 μm in diameter were developed by centrifugal granulat ... | 2018 | 30243502 |
| molecular evolution of porcine epidemic diarrhea virus and porcine deltacoronavirus strains in central china. | porcine epidemic diarrhea virus (pedv) and porcine deltacoronavirus (pdcov) are epizootic swine viruses. to detect and study the evolution of pedv and pdcov in central china (shanxi, henan, hubei province), 70 clinical intestinal and fecal samples from piglets with severe watery diarrhea during august 2015 and june 2016 were collected, tested and analyzed. pedv was more frequently detected by pcr than pdcov. phylogenetic analysis of s genes showed that the 10 pedv strains from this study cluster ... | 2018 | 30265872 |
| detection of neutralizing antibody against porcine epidemic diarrhea virus in subclinically infected finishing pigs. | the purpose of this study was to detect porcine epidemic diarrhea virus (pedv) subclinically infected pigs shipped from non-case farms to slaughterhouses. systematic sampling was conducted at two slaughterhouses. a total of 1,556 blood samples were collected from 80 case and non-case farms from pigs over 6 months old. blood samples were centrifuged to obtain sera. serial serum dilutions were subjected to serological examination for pedv presence using neutralization test (nt). the cut-off titer ... | 2018 | 30282841 |
| the influence of porcine epidemic diarrhea virus on pig small intestine mucosal epithelial cell function. | porcine epidemic diarrhea (ped) is a highly contagious, acute enteric tract infectious disease of pigs (sus domesticus) caused by porcine epidemic diarrhea virus (pedv). ped is characterized by watery diarrhea, dehydration, weight loss, vomiting and death. pedv damages pig intestinal epithelial tissue, causing intestinal hyperemia and atrophy of intestinal villi, with formation of intestinal epithelial cell cytoplasmic vacuoles. since pig small intestinal epithelial cells (iecs) are target cells ... | 2019 | 30284628 |
| correction to: oral administration of inactivated porcine epidemic diarrhea virus activate dcs in porcine peyer's patches. | the original article [1] contained an error whereby the leftmost graph in fig. 1a mistakenly had its x-axis labelled as 'cfst'; this has now been corrected to 'cfse'. | 2018 | 30285740 |
| the characterization of immunoprotection induced by a cdna clone derived from the attenuated taiwan porcine epidemic diarrhea virus pintung 52 strain. | the porcine epidemic diarrhea virus (pedv) poses a great threat to the global swine industries and the unreliable protection induced by the currently available vaccines remains a major challenge. we previously generated a genogroup 2b (g2b) pedv taiwan pintung 52 (pedvpt) strain, pedvpt-p96, and determined its promising host immune response against the virulent pedvpt-p5 strain. to study the attenuation determinants of pedvpt-p96 and establish a pedvpt-p96-based recombinant vector as a vaccine p ... | 2018 | 30287770 |
| resistance to coronavirus infection in amino peptidase n-deficient pigs. | the alphacoronaviruses, transmissible gastroenteritis virus (tgev) and porcine epidemic diarrhea virus (pedv) are sources of high morbidity and mortality in neonatal pigs, a consequence of dehydration caused by the infection and necrosis of enterocytes. the biological relevance of amino peptidase n (anpep) as a putative receptor for tgev and pedv in pigs was evaluated by using crispr/cas9 to edit exon 2 of anpep resulting in a premature stop codon. knockout pigs possessing the null anpep phenoty ... | 2019 | 30315482 |
| detection and differentiation of five diarrhea related pig viruses utilizing a multiplex pcr assay. | porcine viral diarrhea, mainly caused by porcine epidemic diarrhea virus (pedv), transmissible gastroenteritis virus (tgev), porcine group a rotaviruses (rva), porcine group c rotaviruses (rvc) and porcine circovirus 2 (pcv2), is a serious global problem, resulting in substantial economic losses to the swine industry. for fast and reliable diagnosis of the causative agent associated with viral diarrhea in pigs, an inexpensive and easy to perform gel-based multiplex pcr assay was developed in thi ... | 2019 | 30336161 |
| molecular detection of enteric viruses and the genetic characterization of porcine astroviruses and sapoviruses in domestic pigs from slovakian farms. | surveillance and characterization of pig enteric viruses such as transmissible gastroenteritis virus (tgev), porcine epidemic diarrhea virus (pedv), rotavirus, astrovirus (pastv), sapovirus (psav), kobuvirus and other agents is essential to evaluate the risks to animal health and determination of economic impacts on pig farming. this study reports the detection and genetic characterization of pastv, psav in healthy and diarrheic domestic pigs and pedv and tgev in diarrheic pigs of different age ... | 2018 | 30340595 |
| a newly isolated chinese virulent genotype giib porcine epidemic diarrhea virus strain: biological characteristics, pathogenicity and immune protective effects as an inactivated vaccine candidate. | since october 2010, severe porcine epidemic diarrhea (ped) outbreaks caused by highly virulent ped virus (pedv) strains have occurred continuously in the chinese pig population and caused considerable economic losses. although pedv vaccines based on classical pedv strains have been widely used in china in recent years, the morbidity and mortality in piglets remain high. therefore, virulent genotype gii pedv strains that are prevalent in the field should be isolated and used to develop next-gener ... | 2019 | 30342075 |
| microrna-221-5p inhibits porcine epidemic diarrhea virus replication by targeting genomic viral rna and activating the nf-κb pathway. | micrornas (mirnas) are a class of noncoding rnas involved in posttranscriptional regulation of gene expression and many critical roles in numerous biological processes. porcine epidemic diarrhea virus (pedv), the etiological agent of porcine epidemic diarrhea, causes substantial economic loss in the swine industry worldwide. previous studies reported mirna involvement in viral infection; however, their role in regulating pedv infection remains unknown. in this study, we investigated the regulato ... | 2018 | 30380612 |