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salmonella genotype diversity in nonlactating and lactating dairy cows.dairy cows may serve as asymptomatic carriers of salmonella. the potential for herd carrier status increases with herd size, and salmonella shedding may be triggered by stresses placed on the animals. the scope of the current study is to determine the effects lactation may have on salmonella genotypic diversity among detected serotypes. fecal samples were collected on two sampling dates from 60 nonlactating and 60 lactating holstein cows. no serotype was predominant over the two collection dates ...200415508643
surface disinfection tests with salmonella and a putative indicator bacterium, mimicking worst-case scenarios in poultry houses.surface disinfection studies mimicking worst-case scenarios in badly cleaned poultry houses were made with 3 bacterial isolates (salmonella enteritidis, salmonella senftenberg, and enterococcus faecalis), and 3 1% disinfectant solutions, formaldehyde (f; 24.5% vol/vol), glutaraldehyde/benzalkonium chloride (g; bio komplet plus), and a peroxygen compound (p; virkon s), with world health organization (who) standard hard water as a control. materials (concrete paving stones, steel feed chain links, ...200415510546
eight-hour pcr-based procedure for the detection of salmonella in raw oysters.the aim of this study was to evaluate the ability of a nested pcr system to detect salmonella senftenberg in raw oysters. the specific primers of the pcr were derived from the inva gene sequence, essential for salmonella invasiveness into epithelial cells. first, for the extraction of dna, four methods (guanidine isothiocyanate, e.z.n.a. mollusc kit, chelex-100, and lysis with detergents) were compared. a nested pcr method combined with 3.5 h pre-enrichment in buffered peptone water (bpw) and dn ...200515668030
destruction of salmonella senftenberg on the shells of intact eggs by thermoultrasonication.the combined effect of ultrasonic waves and heat treatment applied simultaneously was evaluated on the survival of strains of salmonella senftenberg on shells of intact eggs. this combined process has a higher killing effect than heat treatment alone. the decimal reduction times (d-values) were decreased by 65.2 to 11.1% in the temperature range studied (57.8 to 67 degrees c). in contrast to the effect on salmonella enterica serovar enteritidis in a previous study, thermoultrasonication had no i ...200515830681
use of pulsed-field gel electrophoresis to characterize the genetic diversity and clonal persistence of salmonella senftenberg in mussel processing facilities.salmonella senftenberg was detected in association with persistent contamination events in mussel processing facilities between 1998 and 2002 in spain. a total of 110 isolates from 8 facilities were subjected to molecular typing by pulsed-field gel electrophoresis (pfge). additionally, a selection of epidemiologically unrelated isolates of this serovar originating from human, animal, feed and environmental sources was included in the study. pfge analysis proved to be a useful tool for studying t ...200516109450
investigation of clonal distribution and persistence of salmonella senftenberg in the marine environment and identification of potential sources of contamination.salmonella senftenberg was detected in the coastal areas of galicia (nw spain) in 1998, where it remained the predominant serovar for the next four years. although the overall incidence of this serovar in the zone was lower than 1%, contamination by salmonella serovar senftenberg was located in very specific areas of the ría de arousa, where it persisted for more than five years. a total of 60 salmonella serovar senftenberg isolates, originating from surveillance activities in marine environment ...200416329911
inactivation of salmonella senftenberg 775w by ultrasonic waves under pressure at different water activities.the inactivation of salmonella senftenberg 775w by ultrasonic waves (20 khz, 117 microm) under pressure (175 kpa) treatments at sublethal (manosonication; ms) and lethal temperatures (manothermosonication; mts) in media of different water activities has been investigated. heat decimal reduction time values increased up to eighteen fold when the water activity was decreased from >0.99 to 0.93 at 65 degrees c, but hardly increased the ms resistance. in reduced water activity media (a(w) of 0.96 an ...200616488040
pulsed electric fields cause bacterial envelopes permeabilization depending on the treatment intensity, the treatment medium ph and the microorganism investigated.the relationship between membrane permeabilization and loss of viability by pulsed electric fields (pef) depending on the treatment intensity and the treatment media ph in two gram-positive (lactobacillus plantarum, listeria monocytogenes) and two gram-negative (escherichia coli, salmonella senftenberg 775w) bacterial species has been investigated. loss of membrane integrity was measured as increased uptake of the fluorescent dye propidium iodide (pi). non-permanent/reversible permeabilization w ...200716987561
inactivation of salmonella serovars in liquid whole egg by heat following irradiation treatments.salmonella is a frequent contaminant on eggs and is responsible for foodborne illnesses in humans. ionizing radiation and thermal processing can be used to inactivate salmonella in liquid whole egg, but when restricted to doses that do not affect egg quality, these technologies are only partially effective in reducing salmonella populations. in this study, the effect of ionizing radiation in combination with thermal treatment on the survival of salmonella serovars was investigated. of the six sa ...200616995507
development of thermal surrogate microorganisms in ground beef for in-plant critical control point validation studies.in search of a suitable surrogate microorganism for in-plant critical control point validation, we compared the rates of thermal inactivation of three bacteria, enterococcus faecium b2354, pediococcus parvulus hp, and pediococcus acidilactici lp, to those of listeria monocytogenes and salmonella. ground beef samples containing 4 and 12% fat were inoculated with e. faecium, l. monocytogenes, and salmonella senftenberg 775w and heated at 58, 62, 65, or 68 degrees c. the decimal reduction times (d- ...200717477266
intertransformability of salmonella simsbury and salmonella senftenberg. 194720270737
outbreak of healthcare-associated infection and colonization with multidrug-resistant salmonella enterica serovar senftenberg in florida.in july 1999, a rare strain of multidrug-resistant salmonella enterica serovar senftenberg was isolated from the sputum of a trauma patient. over a 6-year period (1999-2005) in northeast florida, this salmonella serovar spread to 66 other patients in 16 different healthcare facilities as a result of frequent transfers of patients among institutions. to our knowledge, this is the first outbreak of healthcare-associated infection and colonization with a fluoroquinolone-resistant strain of s. senft ...200717564982
inactivation of salmonella senftenberg strain w 775 during composting of biowastes and garden wastes.determination of the minimum requirements (time-temperature relationship and moisture content) that are needed for a sufficient eradication of an indicator organism.200717584452
inactivation of salmonella enteritidis and salmonella senftenberg in liquid whole egg using generally recognized as safe additives, ionizing radiation, and heat.the effect of combining irradiation and heat (i.e., irradiation followed by heat [ir-h]) on salmonella enteritidis and salmonella senftenberg inoculated into liquid whole egg (lwe) with added nisin, edta, sorbic acid, carvacrol, or combinations of these gras (generally recognized as safe) additives was investigated. synergistic reductions of salmonella populations were observed when lwe samples containing gras additives were treated by gamma radiation (0.3 and 1.0 kgy), heat (57 and 60 degrees c ...200717612070
predicting microbial heat inactivation under nonisothermal treatments.the aim of this study was to develop an equation that accurately predicts microbial heat inactivation under nonisothermal treatments at constantly rising heating rates (from 0.5 to 5 degrees c/min) in media with different ph values (4.0 or 7.4). the survival curves of all bacteria (enterococcus faecium, escherichia coli, listeria monocytogenes, salmonella senftenberg 775w, salmonella typhimurium, and staphylococcus aureus) tested under isothermal treatments were nearly linear. for the most heat- ...200717612077
international outbreak of salmonella senftenberg in 2007. 200717868576
modeling the irradiation followed by heat inactivation of salmonella inoculated in liquid whole egg.this study presents mathematical models that describe the inactivation of salmonella enteritidis, salmonella typhimurium, and salmonella senftenberg suspended in liquid whole egg (lwe) by irradiation followed by heat treatments (ir-h treatments). these models also enable prediction of cell injury in salmonella after exposure to ir-h. salmonella viability decreased exponentially (primary model) with heat treating time for all the radiation doses (0, 0.1, 0.3, 0.5, 1.0, and 1.5 kgy) and temperatur ...200717995736
human salmonellosis due to salmonella senftenberg. 194620999310
persistence and growth of different salmonella serovars on pre- and postharvest tomatoes.the interaction of a range of salmonella serovars with pre- and postharvest tomatoes was evaluated. serovars were selected on the basis of previous association in tomato-linked outbreaks of salmonellosis (salmonella javiana, salmonella montevideo, and salmonella newport) or those typically isolated from animal or clinical infections (salmonella dublin, salmonella enteritidis, salmonella hadar, salmonella infantis, salmonella typhimurium, and salmonella senftenberg). salmonella serovars introduce ...200718095423
molecular epidemiological analysis and microbial source tracking of salmonella enterica serovars in a preharvest turkey production environment.epidemiological studies were conducted to source track and delineate horizontal transmission pathways of salmonella serovars in a turkey production environment. salmonella enterica serovar heidelberg (n = 111), salmonella senftenberg (n = 14), salmonella muenster (n = 10), unidentifiable "roughs" (n = 5), salmonella anatum (n = 3), and salmonella worthington (n = 2) were isolated from the birds' cecal and crop contents, litter, environmental swabs, drinkers, and feed samples. these strains (n = ...200818361686
the yersinia high pathogenicity island is present in salmonella enterica subspecies i isolated from turkeys.the yersinia high pathogenicity island (hpi) encodes a yersiniabactin-mediated iron acquisition system present in highly pathogenic strains of yersinia and several members of the enterobacteriaceae. in this study, 420 salmonellae representing multiple serovars recovered from diverse hosts were investigated for the presence of the hpi. the isolates were initially screened via pcr with primers specific for irp2, a conserved gene involved in yersiniabactin biosynthesis. seventeen isolates produced ...200818495411
[survival of salmonella in spices and growth in cooked food].contamination of spices with pathogens has been reported worldwide, and salmonella might result in foodborne infections. in this study, we investigated the survival of salmonella in black pepper and red pepper, and the growth of the surviving salmonella in cooked food. salmonella enteritidis, salmonella weltevreden and salmonella senftenberg were inoculated into spices, and their survival during storage was examined. in black pepper, s. enteritidis was no longer viable after storage for 28 days, ...200818503241
persistence of salmonella senftenberg in poultry production environments and investigation of its resistance to desiccation.most salmonella serovars, including salmonella enterica subsp. enterica serovar senftenberg (s. senftenberg), are tolerant to desiccation and able to colonize and persist in feed mills. in addition, they may survive cleaning and disinfection procedures used on poultry farms. the present study was conducted to investigate the survival of s. senftenberg in broiler parent stock farms and broiler farms. the isolates from one of the parent stock farms investigated only differed by a single band in fl ...200818622860
interaction of salmonella enterica with basil and other salad leaves.contaminated salad leaves have emerged as important vehicles for the transmission of enteric pathogens to humans. a recent outbreak of salmonella enterica serovar senftenberg (s. senftenberg) in the united kingdom has been traced to the consumption of contaminated basil. using the outbreak strain of s. senftenberg, we found that it binds to basil, lettuce, rocket and spinach leaves showing a pattern of diffuse adhesion. flagella were seen linking s. senftenberg to the leaf epidermis, and the del ...200918830276
packed with salmonella--investigation of an international outbreak of salmonella senftenberg infection linked to contamination of prepacked basil in 2007.salmonella senftenberg is uncommon in the united kingdom. in january-june 2007, the health protection agency reported on 55 primary human cases of salmonella senftenberg in england and wales. in may 2007, fresh basil sold in the united kingdom was found to be contaminated with salmonella senftenberg. we launched an investigation to elucidate the cause of this outbreak. isolates were examined using plasmid profiling and pulsed-field gel electrophoresis, and the outbreak strain (ssftxb.0014) was i ...200818851676
thermal treatment for pathogen inactivation as a risk mitigation strategy for safe recycling of organic waste in agriculture.thermal treatment at temperatures between 46.0°c and 55.0°c was evaluated as a method for sanitization of organic waste, a temperature interval less commonly investigated but important in connection with biological treatment processes. samples of dairy cow feces inoculated with salmonella senftenberg w775, enterococcus faecalis, bacteriophage ϕx174, and porcine parvovirus (ppv) were thermally treated using block thermostats at set temperatures in order to determine time-temperature regimes to ac ...201425035917
relationship between membrane fatty acid composition and heat resistance of acid and cold stressed salmonella senftenberg cect 4384.this study evaluates the adaptative response to heat (63 degrees c) and the modifications in membrane fatty acid composition of salmonella senftenberg after its growth in an acidified medium and after its exposure to combinations of acid and cold stresses. cells were grown in brain heart infusion (bhi) buffered at ph 7.0 and acidified up to ph 4.5 (fresh cultures) and kept at refrigeration temperature (4 degrees c) for 7 days (refrigerated cultures). the results indicate that previous adaptation ...200919269580
pathogen reduction in minimally managed composting of bovine manure.spread of manure pathogens is of considerable concern due to use of manure for land application. in this study, the effects of four static pile treatment options for bovine manure on die-off of a generic escherichia coli, e. coli o157:h7 surrogate, salmonella senftenberg, salm. typhimurium, and listeria monocytogenes were evaluated. bovine manure spiked with these bacteria were placed in cassettes at the top, middle, and bottom sections of four static pile treatments that reflect minimal changes ...201425151442
relationship between the physicochemical properties of nonchitinolytic listeria and salmonella and their attachment to shrimp carapace.listeria and salmonella are important foodborne pathogens normally associated with the shrimp production chain. this study investigated the potential of salmonella typhimurium, salmonella senftenberg, and listeria monocytogenes (scott a and v7) to attach to and colonize shrimp carapace. attachment and colonization of listeria and salmonella were demonstrated. shrimp abdominal carapaces showed higher levels of bacterial attachment (p < 0.05) than did head carapaces. listeria consistently exhibite ...200919610328
a comparative study of thermal and acid inactivation kinetics in fruit juices of salmonella enterica serovar typhimurium and salmonella enterica serovar senftenberg grown at acidic conditions.acid and heat inactivation in orange and apple juices of salmonella enterica serovar typhimurium colección española de cultivos tipo (i.e., spanish type culture collection) 443 (cect 443) (salmonella typhimurium) and s. enterica serovar senftenberg cect 4384 (salmonella senftenberg) grown in buffered brain heart infusion (ph 7.0) and acidified brain heart infusion up to ph 4.5 with acetic, citric, lactic, and hydrochloric acids was evaluated. acid adaptation induced an adaptive response that inc ...200919694554
emergence of extended-spectrum beta-lactamases and ampc-type beta-lactamases in human salmonella isolated in spain from 2001 to 2005.to study the resistance to third-generation cephalosporins in salmonella strains isolated from humans in a 5 year period in spain, and to identify the responsible genes and their dissemination.200919815633
temperature and deactivation of microbial faecal indicators during small scale co-composting of faecal matter.small scale co-composting of faecal matter from dry toilet systems with shredded plant material and food waste was investigated in respect to heat development and deactivation of faecal indicators under tropical semiarid conditions. open (uncovered) co-composting of faecal matter with shredded plant material alone did not generate temperatures high enough (<55 degrees c) to reduce the indicators sufficiently. the addition of food waste and confinement in chambers, built of concrete bricks and wo ...201019889525
salmonella serovars and their antimicrobial resistance in british turkey flocks in 1995 to 2006.serovar and antimicrobial resistance data from the scanning surveillance of british turkey flocks for salmonella between 1995 and 2006 were analysed and compared with prevalence data from other livestock and animal feed. a total of 2753 incidents of 57 different serovars were reported. the five most prevalent serovars were salmonella typhimurium (20.8%), salmonella newport (14.7%), salmonella derby (10.6%), salmonella indiana (8.3%) and salmonella agona (6.4%). s. typhimurium reports peaked in t ...200919937522
human salmonellosis due to salmonella senftenberg. 194619970904
the effects of temperature, chlorine and acids on the survival of listeria and salmonella strains associated with uncooked shrimp carapace and cooked shrimp flesh.the purpose of this study was to assess the influence of the association of listeria and salmonella with shrimp surfaces on the effects of temperature, chlorine and acids on their survival. planktonic, attached and colonized cells of listeria monocytogenes scott a, l. monocytogenes v7, salmonella senftenberg 1734b and s. typhimurium atcc 14028 were challenged with high (50 degrees , 60 degrees and 70 degrees c) and low (4 degrees c) temperature, 100 ppm sodium hypochlorite solution, and acetic, ...201020141943
[a 14-year-old healthy boy with splenic abscess due to salmonella enterica serovar senftenberg].salmonella enterica serovar senftenberg may very rarely cause splenic abscess, which can be diagnosed using gallium scintigraphy and drained. a 14-year-old boy admitted for stomachache, diarrhea and fever and diagnosed from his symptoms as having enteritis did not respond when treated with fosfomycin, meropenem, and clindamycin. a low-density splenic area seen in abdominal computed tomography on admission did not show contrast medium enhancement. gallium scintigraphy on hospital day 10, however, ...201020170018
[study on the epidemiological characteristics and molecular typing of salmonella enterica subsp. enterica serovar senftenberg in shanghai].to study the molecular epidemiological characteristics of salmonella enterica subsp. enterica serovar senftenberg (salmonella senftenberg) in shanghai, from 2006 to 2007.200920193231
characterization of multidrug-resistant, qnrb2-positive and extended-spectrum-beta-lactamase-producing salmonella concord and salmonella senftenberg isolates.to characterize plasmids and resistance genes of multidrug-resistant (mdr) salmonella senftenberg and salmonella concord isolated from patients in the netherlands.201020200038
fatal human salmonellosis due to salmonella senftenberg. 194520292079
salmonella senftenberg infections and fennel seed tea, serbia. 201020409401
hatchery hygiene evaluation by microbiological examination of hatchery samples.this study was conducted to investigate the bacterial contamination of air and the surface of equipment and facilities in hatchery. in addition, the inhibitory effects of formaldehyde application methods on aerosol bacterial counts in the hatchers were also investigated. in the operating hatchers, the contamination of air by aerobic bacteria, coliform, and fungi was high, measuring over 300 cfu/63.6 cm(2). in the egg sorting room, contamination was moderate, whereas in the remaining sampling sit ...201020548066
reduction of salmonella on inoculated almonds exposed to hot oil.the heat resistance of salmonella inoculated onto almonds was determined after immersion in hot oil. whole almonds were inoculated with salmonella enteritidis pt 30 or salmonella senftenberg 775w and heated in oil. after heating, almonds were drained, transferred to cold tryptic soy broth, and mixed with a stomacher, and samples were plated onto tryptic soy and bismuth sulfite agars. salmonella survivor inactivation curves were upwardly concave. rapid reductions of 2.9, 3.0, or 3.6 log cfu/g for ...201020615336
a collaborative study on a method to detect salmonella in food.fourteen laboratories with expertise in salmonella detection in food joined in a collaborative study. the laboratories performed qualitative analyses of ground pork samples using the proposed detection method. salmonella typhimurium (hydrogen sulfide-producing strain) and salmonella senftenberg (hydrogen sulfide-nonproducing strain) were used for inoculation. three levels of salmonella contamination were used for the study (0, 1-10, and 11-100 cfu/25 g). we evaluated the presence of salmonella i ...201020616435
evaluation of pulsed electric fields technology for liquid whole egg pasteurization.this investigation evaluated the lethal efficiency of pulsed electric fields (pefs) to pasteurize liquid whole egg (lwe). to achieve this aim, we describe the inactivation of salmonella enteritidis and the heat resistant salmonella senftenberg 775 w in terms of treatment time and specific energy at electric field strengths ranging from 20 to 45 kv/cm. based on our results, the target microorganism for this technology in lwe varied with intensity of the pef treatment. for electric field strengths ...201020688225
leaching of salmonella enterica in clay columns comparing two manure application methods.transfer of zoonotic bacterial pathogens through intact soil columns was monitored in an outdoor lysimeter over 36 d. manure spiked with salmonella enterica serovar senftenberg was applied to either the soil surface or injected 0.08 m into the soil to compare leaching associated with the two manure application methods. the highest concentrations of s. enterica (up to 60,000 s. enterica cfu/ml) were detected on day 1 in the first drainage samples, with measurable but declining concentrations pers ...201021039451
thermal resistance of salmonella senftenberg. 196621090498
fate of pathogenic bacteria associated with production of pickled sausage by using a cold fill process.preservation by pickling has been used for many years to extend the shelf life of various types of food products. by storing meat products in a brine solution containing an organic acid, salt, spices, as well as other preservatives, the ph of the product is reduced, thus increasing the safety and shelf life of the product. pickling may involve the use of heated brines to further add to the safety of the food product. when precooked, ready-to-eat (rte) sausages are pickled with a heated brine sol ...201628221846
potential for transfer of escherichia coli o157:h7, listeria monocytogenes and salmonella senftenberg from contaminated food waste derived compost and anaerobic digestate liquid to lettuce plants.the diversion of food wastes from landfill to sustainable disposal methods, such as composting and anaerobic digestion, has led to an increase in the soil amendment products that are now commercially available and which are derived from both of these processes. the use of such products as soil amendments during the production of ready-to-eat (rte) crops is increasing worldwide. the aim of this study was to investigate the potential of three well-recognised bacterial pathogens of importance to pu ...201627375239
combination of pulsed electric fields, mild heat and essential oils as an alternative to the ultrapasteurization of liquid whole egg.the production of microbiologically safe liquid whole egg (lwe) by industrial ultrapasteurization is restricted by the high thermal sensitivity of lwe components. this research proposes an alternative treatment based on the application of pulsed electric fields (pef) and mild heat, in the presence of natural essential oils (eos) or their individual components (ics). the obtained results indicate that the successive application of pef (25kv/ and 100kj/kg) followed by heat (60°c during 3.5') to lw ...201425146463
genome sequence of the thermotolerant foodborne pathogen salmonella enterica serovar senftenberg atcc 43845 and phylogenetic analysis of loci encoding increased protein quality control mechanisms.salmonella enterica subsp. enterica bacteria are important foodborne pathogens with major economic impact. some isolates exhibit increased heat tolerance, a concern for food safety. analysis of a finished-quality genome sequence of an isolate commonly used in heat resistance studies, s. enterica subsp. enterica serovar senftenberg 775w (atcc 43845), demonstrated an interesting observation that this strain contains not just one, but two horizontally acquired thermotolerance locus homologs. these ...201728293682
salmonella senftenberg adaptation to linalool and its association with antibiotic resistance and environmental persistence.a clinical isolate of salmonella enterica serovar senftenberg, isolated from an outbreak linked to the herb ocimum basilicum l. (basil), has been shown resistance to basil oil and to the terpene alcohol linalool. to better understand how human pathogens might develop resistance to linalool and to investigate the association of this resistance with resistance to different antimicrobial agents, selective pressure was applied to the wild-type strain by sequential exposure to increasing concentratio ...201728258149
homologous prime-boost immunization with live attenuated salmonella enterica serovar senftenberg and its preventive efficacy against experimental challenge with various strains of s. senftenberg.the heterogeneity observed regarding persistence, and subsequent fecal shedding pattern of the salmonella senftenberg (s. senftenberg) serovar in chicken's calls for development of the optimized immunization strategy which can provide protection against various s. senftenberg isolated. optimization of an immunization strategy with a live attenuated s. senftenberg (δlon and δcpxr) vaccine candidate (jol1587) was undertaken in this study to evaluate the ability of a homologous prime-boost immuniza ...201728143524
leaching of human pathogens in repacked soil lysimeters and contamination of potato tubers under subsurface drip irrigation in denmark.the risk for contamination of potatoes and groundwater through subsurface drip irrigation with low quality water was explored in 30 large-scale lysimeters containing repacked coarse sand and sandy loam soils. the human pathogens, salmonella senftenberg, campylobacter jejuni and escherichia coli o157:h7, and the virus indicator salmonella typhimurium bacteriophage 28b, were added weekly through irrigation tubes for one month with low irrigation rates (8 mm per week). in the following six months l ...201121762945
new delhi metallo-β-lactamase-producing enterobacteriaceae, united states.we characterized 9 new delhi metallo-β-lactamase-producing enterobacteriaceae (5 klebsiella pneumoniae, 2 escherichia coli, 1 enterobacter cloacae, 1 salmonella enterica serovar senftenberg) isolates identified in the united states and cultured from 8 patients in 5 states during april 2009-march 2011. isolates were resistant to β-lactams, fluoroquinolones, and aminoglycosides, demonstrated mics ≤1 µg/ml of colistin and polymyxin, and yielded positive metallo-β-lactamase screening results. eight ...201323731823
efficacy of ultraviolet (uv-c) light in a thin-film turbulent flow for the reduction of milkborne pathogens.nonthermal technologies are being investigated as viable alternatives to, or supplemental utilization, with thermal pasteurization in the food-processing industry. in this study, the effect of ultraviolet (uv)-c light on the inactivation of seven milkborne pathogens (listeria monocytogenes, serratia marcescens, salmonella senftenberg, yersinia enterocolitica, aeromonas hydrophila, escherichia coli, and staphylococcus aureus) was evaluated. the pathogens were suspended in ultra-high-temperature w ...201525884367
effects of spring season solar drying process on sanitation indicators in sewage sludge and potential as a method for fertilizer production.the agricultural use of sewage sludge is possible on condition of maintaining microbiological and parasitological standards, and one of the most modern methods improving its sanitary state is solar drying. in the presented study, the effect of this process on the elimination of indicator microorganisms (escherichia coli, salmonella senftenberg w775, enterococcus spp.) and eggs of ascaris suum introduced into the biomass of sludge was examined. the experiment was carried out in the spring period ...201323540205
recovery estimation of dried foodborne pathogens is directly related to rehydration kinetics.drying is a common process which is used to preserve food products and technological microorganisms, but which is deleterious for the cells. the aim of this study is to differentiate the effects of drying alone from the effects of the successive and necessary rehydration. rehydration of dried bacteria is a critical step already studied in starter culture but not for different kinetics and not for pathogens. in the present study, the influence of rehydration kinetics was investigated for three fo ...201627494169
attributes of carbapenemase encoding conjugative plasmid pndm-sal from an extensively drug-resistant salmonella enterica serovar senftenberg.a carbapenem resistant salmonella enterica serovar senftenberg isolate bch 2406 was isolated from a diarrheal child attending an outpatient unit of b.c. roy hospital in kolkata, india. this isolate was positive for the bla ndm-1 in the pcr assay, which was confirmed by amplicon sequencing. except for tetracycline, this isolate was resistant to all the tested antimicrobials. the bla ndm-1 was found to be located on a 146.13-kb mega plasmid pndm-sal, which could be conjugally transferred into esch ...201526441902
development of challenge models to evaluate the efficacy of a vaccine to reduce carriage of salmonella in peripheral lymph nodes of cattle.because challenge models to infect peripheral lymph nodes (plns) with salmonella have not been reported, we performed a series of experiments to develop and refine challenge models to evaluate an intervention applied at the animal level and to provide initial estimates of efficacy of an intervention (i.e., a vaccine) to aid in the design of future studies. in each of four experiments, steers (control or vaccinated) were inoculated with salmonella strains montevideo or newport, and in experiment ...201323834803
use of the filter-sandwich carriers in continuous effectiveness monitoring of slurry treatment methods as an element improving biosafety in agriculture.slurry, due to high microbiological contamination, requires hygienization before spreading. the agricultural usage of treated slurry has to guarantee biosafety. therefore, constant monitoring of the slurry treatment process should be conducted. the use of filter-sandwich carriers seems to be a prospective solution. the aim of the research was to test whether filter-sandwich carriers influence the survivability of microorganisms during the slurry hygienization process and hence, whether they are ...201323772570
relationship of triamine-biocide tolerance of salmonella enterica serovar senftenberg to antimicrobial susceptibility, serum resistance and outer membrane proteins.a new emerging phenomenon is the association between the incorrect use of biocides in the process of disinfection in farms and the emergence of cross-resistance in salmonella populations. adaptation of the microorganisms to the sub-inhibitory concentrations of the disinfectants is not clear, but may result in an increase of sensitivity or resistance to antibiotics, depending on the biocide used and the challenged salmonella serovar. exposure of five salmonella enterica subsp. enterica serovar se ...201728696348
a comparative study on invasion, survival, modulation of oxidative burst, and nitric oxide responses of macrophages (hd11), and systemic infection in chickens by prevalent poultry salmonella serovars.poultry is a major reservoir for foodborne salmonella serovars. salmonella typhimurium, salmonella enteritidis, salmonella heidelberg, salmonella kentucky, and salmonella senftenberg are the most prevalent serovars in u.s. poultry. information concerning the interactions between different salmonella species and host cells in poultry is lacking. in the present study, the above mentioned salmonella serovars were examined for invasion, intracellular survival, and their ability to modulate oxidative ...201223067396
nanoemulsified d-limonene reduces the heat resistance of salmonella senftenberg over 50 times.salmonella senftenberg is a pathogen agent causative of foodborne disease and it is considered the most heat-resistant serovar within this genus. food industries use heat treatment and chemical antimicrobials in order to eliminate this microorganism in food, but consumers prefer natural antimicrobials as essential oils and their components. this study evaluates the combined effect of thermal treatments and different concentrations of d-limonene nanoemulsion on the inactivation of salmonella (s.) ...201728336899
thermal inactivation of salmonella spp. in pork burger patties.predictive models, to estimate the reduction in escherichia coli o157:h7 concentration in beef burgers, have been developed to inform risk management decisions; no analogous model exists for salmonella spp. in pork burgers. in this study, "extra lean" and "regular" fat pork minces were inoculated with salmonella spp. (salmonella 4,[5],12,i:-, salmonella senftenberg and salmonella typhimurium) and formed into pork burger patties. patties were cooked on an electric skillet (to imitate home cooking ...201626686598
epizootiologic role of feeds in the epidemiology of salmonella senftenberg contamination in commercial layer farms in eastern japan.in total, 40 commercial layer farms and 32 replacement pullet farms with a combined population of 7.5 million adult layers and 6.6 million replacement pullets from six prefectures in eastern japan were investigated for salmonella senftenberg contamination. we randomly collected 17,956 environmental samples, 5816 feed samples, and 218,470 egg samples from commercial layer farms; and 427 feed samples and 2896 environmental samples from replacement pullet farms. we monitored all samples for salmone ...201223050468
salmonella senftenberg osteomyelitis. 199928775610
modeling the heat inactivation of foodborne pathogens in milk powder: high relevance of the substrate water activity.due to the ability of foodborne pathogens to survive in low moisture foods, the decontamination of these products is an important issue in food hygiene. up to now, such decontamination has mostly been achieved through empirical methods. the intention of this work is to establish a more rational use of heat treatment cycles. the effects of thermal treatment cycles on the inactivation of dried salmonella typhimurium, salmonella senftenberg, cronobacter sakazakii and escherichia coli were assessed. ...201728784519
characterization and evaluation of a salmonella enterica serotype senftenberg mutant created by deletion of virulence-related genes for use as a live attenuated vaccine.natural infections of chickens with salmonella enterica subsp. enterica serovar senftenberg (s. senftenberg) are characterized by low-level intestinal invasiveness and insignificant production of antibodies. in this study, we investigated the potential effects of lon and cpxr gene deletions on the invasiveness of s senftenberg into the intestinal epithelium of chickens and its ability to induce an immune response, conferring protection against s senftenberg infection. with the allelic exchange m ...201627489135
genomic and phenotypic analyses reveal the emergence of an atypical salmonella enterica serovar senftenberg variant in china.human infections with salmonella enterica subspecies enterica serovar senftenberg are often associated with exposure to poultry flocks, farm environments, or contaminated food. the recent emergence of multidrug-resistant isolates has raised public health concerns. in this study, comparative genomics and phenotypic analysis were used to characterize 14 salmonella senftenberg clinical isolates recovered from multiple outbreaks in shenzhen and shanghai, china, between 2002 and 2011. single-nucleoti ...201627225410
mechanisms of resistance to linalool in salmonella senftenberg and their role in survival on basil.fresh produce contaminated with human pathogens raises vital and ecological questions about bacterial survival strategies. such occurrence was basil harboring salmonella enterica serovar senftenberg that caused an outbreak in 2007. this host was unanticipated due to its production of antibacterial substances, including linalool. we show that linalool perforates bacterial membranes, resulting in increased permeability and leakage of vital molecules. it also inhibits cell motility and causes bacte ...201626914987
inactivation of salmonella senftenberg, salmonella typhimurium and salmonella tennessee in peanut butter by 915 mhz microwave heating.this study evaluated the efficacy of a 915 mhz microwave with 3 different levels to inactivate 3 serovars of salmonella in peanut butter. peanut butter inoculated with salmonella enterica serovar senftenberg, s. enterica serovar typhimurium and s. enterica serovar tennessee were treated with a 915 mhz microwave with 2, 4 and 6 kw and acid and peroxide values and color changes were determined after 5 min of microwave heating. salmonella populations were reduced with increasing treatment time and ...201626678129
characterization of antimicrobial resistance in salmonella enterica strains isolated from brazilian poultry production.antimicrobial resistance profiles and presence of resistance determinants and integrons were evaluated in salmonella enterica strains from brazilian poultry. the analysis of 203 isolates showed that those from the poultry environment (88 isolates) were significantly more resistant to antimicrobials than isolates from other sources, particularly those isolated from poultry by-product meal (106 isolates). thirty-seven isolates were resistant to at least three antimicrobial classes. class 1 integro ...201526337044
older leaves of lettuce (lactuca spp.) support higher levels of salmonella enterica ser. senftenberg attachment and show greater variation between plant accessions than do younger leaves.salmonella can bind to the leaves of salad crops including lettuce and survive for commercially relevant periods. previous studies have shown that younger leaves are more susceptible to colonization than older leaves and that colonization levels are dependent on both the bacterial serovar and the lettuce cultivar. in this study, we investigated the ability of two lactuca sativa cultivars (saladin and iceberg) and an accession of wild lettuce (l. serriola) to support attachment of salmonella ente ...201525953858
emergence and prevalence of non-h2s-producing salmonella enterica serovar senftenberg isolates belonging to novel sequence type 1751 in china.salmonella enterica serovar senftenberg is a common nontyphoidal salmonella serotype which causes human salmonella infections worldwide. in this study, 182 s. senftenberg isolates, including 17 atypical non-hydrogen sulfide (h2s)-producing isolates, were detected in china from 2005 to 2011. the microbiological and genetic characteristics of the non-h2s-producing and selected h2s-producing isolates were determined by using pulsed-field gel electrophoresis (pfge), multilocus sequence typing (mlst) ...201424829240
fate of salmonella senftenberg in broiler chickens evaluated by challenge experiments.experimental and epidemiological evidence has indicated the respiratory route to be a potential portal of entry for salmonellas in poultry. the purpose of this study was to evaluate and compare the infectivity of salmonella enterica serovar senftenberg following oral gavage, intratracheal or intravenous challenge in chickens. seven-day-old chicks were challenged with either 10(4) or 10(6) colony-forming units of s. senftenberg per chick by oral gavage, intratracheal or intravenous challenge, res ...201424820534
serotype profile and molecular characterization of antimicrobial resistance in non-typhoidal salmonella isolated from gastroenteritis cases over nine years.resistance to third-generation cephalosporins in non-typhoidal salmonella (nts) is emerging worldwide. we report the occurrence of extended-spectrum beta-lactamase (esbl) phenotypes in 53.4 % of nts isolated over a period of nine years from gastroenteritis cases. esbl and ampc co-production was observed in 21 % of the isolates. occurrence of blactx-m-15 and blacmy-2 resistance genes was observed in 11.6 % and 37 % of the isolates respectively. overall, salmonella enterica serovar senftenberg was ...201424149623
resistance to essential oils affects survival of salmonella enterica serovars in growing and harvested basil.the number of outbreaks of food-borne illness associated with consumption of fresh products has increased. a recent and noteworthy outbreak occurred in 2007. basil contaminated with salmonella enterica serovar senftenberg was the source of this outbreak. since basil produces high levels of antibacterial compounds the aim of this study was to investigate if the emerging outbreak reflects ecological changes that occurred as a result of development of resistance to ingredients of the basil oil. we ...201323648052
extremely drug-resistant salmonella enterica serovar senftenberg infections in patients in zambia.two cases of extremely drug-resistant salmonella enterica serovar senftenberg isolated from patients in zambia were investigated by utilizing mic determinations and whole-genome sequencing. the isolates were resistant to, and harbored genes toward, nine drug classes, including fluoroquinolones and extended-spectrum cephalosporins, contained two plasmid replicons, and differed by 93 single-nucleotide polymorphisms.201323077128
[characteristics of salmonella enterica serovar senftenberg lacking salmonella pathogenicity island 1].to study the characteristics of the strains of salmonella enterica (s. enterica) serovar senftenberg lacking salmonella pathogenicity island 1 (spi-1).201122321589
assessing transmission of salmonella to bovine peripheral lymph nodes upon horn fly feeding.biting arthropods are implicated in the transdermal transmission of salmonella to bovine peripheral lymph nodes, and such contamination can contribute to increased salmonella prevalence in processed beef. since horn flies can acquire salmonella and then excrete the bacteria in their feces, on-animal fly infestations were conducted in this study to assess whether horn flies have a role in this bacterial transmission. three salmonella serotypes were used to assess fly acquisition from and excretio ...201627357032
effect of water activity on the thermal tolerance and survival of salmonella enterica serovars tennessee and senftenberg in goat's milk caramel.the low thermal tolerance of salmonella enterica in foods with intermediate moisture levels, such as caramel sauces, ensures that mild heat treatment is sufficient to achieve 5-log reductions of this pathogen. this treatment mitigates the risk posed by salmonellae in raw materials; however, recontamination might occur because of survival of the pathogen in products that are not heated before consumption. this study was conducted to evaluate the effect of water activity (aw) on the thermal tolera ...201728459616
efficacy of traditional almond decontamination treatments and electron beam irradiation against heat-resistant salmonella strains.two outbreaks of salmonellosis were linked to the consumption of raw almonds from california in 2001 and 2004. as a result, federal regulations were developed, which mandate that all almonds grown in california must be treated with a process that results in a 4-log reduction of salmonella. because most of the technologies approved to treat almonds rely on the application of heat to control salmonella, an evaluation of alternative technologies for inactivating heat-resistant salmonella enteritidi ...201626939646
a temporal study of salmonella serovars from fluff samples from poultry breeder hatcheries in ontario between 1998 and 2008.the objectives of this study were to determine the prevalence of salmonella, trends, major serovars, and their clusters from fluff samples, in poultry hatcheries in ontario between 1998 and 2008. multi-level logistic regression modelling with random effects for hatchery and sampling visit (day on which samples were collected from a hatchery) was used to identify factors [poultry breeder type, year (trend), and season] associated with the prevalence of salmonella and a cluster detection test was ...201323814352
combined effect of nisin, carvacrol and a previous thermal treatment on the growth of salmonella enteritidis and salmonella senftenberg.the aim of this study was to evaluate the combined effect of a previous mild heat treatment (15 min at 55 ) with the use of antimicrobials, nisin and carvacrol, on the growth of salmonella enteritidis and salmonella senftenberg. natural antimicrobials, alone or combined with a previous mild heat treatment, affected the growth of these two serovars in tryptone soy broth at 37 . increasing concentrations of carvacrol had a significant effect on both growth rate and lag phase duration of both strai ...201323729416
sanitization efficacy of anaerobic digestion and aeration of slurry from the aspect of limiting emission of salmonella into the environment.the aim of this study was to estimate the usefulness of mesophilic anaerobic digestion and aeration for sanitization of slurry from the aspect of limiting transmission of salmonella into the environment. material for the study was fresh pig slurry. collected samples were subjected to anaerobic digestion at 35°c and aeration with an initial temperature of 35°c. the efficacy of both methods was examined based on determination of the elimination rate and theoretical time of survival of salmonella s ...201223020034
inactivation of salmonella spp. in liquid whole egg using pulsed electric fields, heat, and additives.this paper evaluates the lethal effectiveness on 7 different salmonella serovars of the application, in static and continuous conditions, of pulsed electric fields (pef) followed by heat treatments in liquid whole egg (lwe) with additives (edta or triethyl citrate-tc-). compared to heat treatments, the pef (25 kv/cm and 75-100 kj/kg) followed by heat (52°c/3.5', 55°c/2', or 60°c/1') in lwe with 2% tc permitted the reduction of heat treatment time from 92 fold at 52°c to 3.4 fold at 60°c, and 4.8 ...201222365352
surveillance of salmonella prevalence in animal feeds and characterization of the salmonella isolates by serotyping and antimicrobial susceptibility.this article presents the surveillance data from the feed contaminants program (2002-2009) and salmonella assignment (2007-2009) of the u.s. food and drug administration (fda), which monitor the trend of salmonella contamination in animal feeds. a total of 2,058 samples were collected from complete animal feeds, feed ingredients, pet foods, pet treats, and supplements for pets in 2002-2009. these samples were tested for the presence of salmonella. those that were positive for salmonella underwen ...201222735034
extra-intestinal salmonellosis in a tertiary care center in south india.the present study is a retrospective analysis of a total of 36 cases of bacteriologically proven extra-intestinal salmonellosis, managed at nizam's institute of medical sciences, between 1987 and 2012 (25 years). the extra-intestinal sites involved were the skin, cerebrum, spleen, ovary, synovium, and the skeletal muscle.201425022292
antimicrobial susceptibility and molecular typing of salmonella senftenberg isolated from humans and other sources in shanghai, china, 2005 to 2011.salmonella senftenberg is an important nontyphoidal salmonella serovar that causes gastrointestinal disease worldwide. in total, 130 salmonella senftenberg strains obtained from humans, food, and the environment in shanghai, people's republic of china, were characterized for antimicrobial susceptibility and subjected to molecular typing. our findings indicated that most (96 of 130, 73.8%) of the strains were susceptible to all 13 antimicrobial compounds tested, whereas only two strains (1.5%) we ...201728221879
selection of indigenous indicator micro-organisms for validating desiccation-adapted salmonella reduction in physically heat-treated poultry litter.the thermal resistance of desiccation-adapted salmonella senftenberg 775/w was compared with those of indigenous enterococci and total aerobic bacteria in poultry litter.201728374481
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