| molecular cloning and allergenicity of pen j 1, a major allergen of kuruma prawn, penaeus japonicus. | tropomyosins have been identified as a common allergen in crustaceans, but their allergenicity is not well understood. in the present study, we isolated an allergen, pen j 1, a tropomyosin from kuruma prawn penaeus japonicus, and determined its n-terminal amino acid sequence. the cdna encoding the allergen was cloned by 5'- and 3'-rapid amplification of cdna ends (race), and was found to code for a protein which consists of 284 amino acid residues. sequencing analyses indicated for the first tim ... | 2009 | 19352041 |
| molecular cloning and phylogenetic analysis on alpha2-macroglobulin (alpha2-m) of white shrimp litopenaeus vannamei. | the full-length sequence of alpha2-macroglobulin (alpha2-m) was cloned from the hemocytes of white shrimp litopenaeus vannamei by reverse transcription polymerase chain reaction (rt-pcr), cloning and sequencing of overlapping pcr, and the rapid amplification of cdna ends method. the alpha2-m cdna consists of 4682bp with an open reading frame of 4479bp, a 52-bp 5'-untranslated region, and a 132-bp 3'-untranslated region. the predicted molecular mass of the mature protein (1475 amino acids) is 167 ... | 2008 | 17706773 |
| a simple non-enzymatic method for the preparation of white spot syndrome virus (wssv) dna from the haemolymph of marsupenaeus japonicus using fta matrix cards. | white spot syndrome virus (wssv) is an important shrimp pathogen responsible for large economic losses for the shrimp culture industry worldwide. the nucleic acids of the virus must be adequately preserved and transported from the field to the laboratory before molecular diagnostic analysis is performed. here, we developed a new method to isolate wssv-dna using flinders technology associates filter paper (fta matrix card; whatman) without centrifugation or hazardous steps involved. fta technolog ... | 2009 | 19476557 |
| regulation of phagocytosis against bacterium by rab gtpase in shrimp marsupenaeus japonicus. | rab gtpases, members of the ras superfamily, play important roles in phagosome formation and maturation. however, the involvement of rab protein in phagocytosis against invading pathogens in crustacean remains unknown. in the present study, the rnai and mrna overexpression assays were conducted to elucidate the function of shrimp rab gene (designated as pjrab) in hemocytic phagocytosis against bacterium. the results indicated that the phagocytic percentage and phagocytic index using fitc-labeled ... | 2008 | 18650102 |
| comparison of antiviral efficiency of immune responses in shrimp. | the antiviral effectiveness of three major immune responses including phagocytosis, apoptosis and propo system in the shrimp marsupenaeus japonicus was characterized. it was found that the suppression of apoptosis and phagocytosis in vivo by their corresponding inhibitors resulted in the increase of white spot syndrome virus (wssv) copies and shrimp mortality, whereas the inhibition of phenol oxidase generated the least influence on wssv infection and shrimp mortality. these results suggest that ... | 2008 | 18721886 |
| molecular cloning and characterization of a cytosolic manganese superoxide dismutase (cytmnsod) and mitochondrial manganese superoxide dismutase (mtmnsod) from the kuruma shrimp marsupenaeus japonicus. | a cytosolic manganese superoxide dismutase (cytmnsod) gene and a mitochondrial manganese superoxide dismutase (mtmnsod) gene were cloned from the kuruma shrimp marsupenaeus japonicus using reverse transcription-polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends (race) method. the open reading frame (orf) of cytmnsod is 861 bp and encodes a 287 amino acids (aa) protein with a 61 aa leader sequence, whereas the orf of mtmnsod is 663 bp and encodes a 221 aa protein with a 21 aa ... | 2010 | 19835962 |
| predicted sub-populations in a marine shrimp proteome as revealed by combined est and cdna data from multiple penaeus species. | abstract: | 2010 | 21067619 |
| a convenient method for preparation of biologically active recombinant chh of the kuruma prawn, marsupenaeus japonicus, using the bacterial expression system. | crustacean hyperglycemic hormone (chh) not only plays an important role in the modulation of hemolymph glucose level but also functions in other biological events including molting, reproduction and stress response. of the six chhs characterized in marsupenaeus japonicus, an expression system for recombinant pej-sgp-vii (rpej-sgp-vii-amide) has not yet been established. here, we established a procedure using a nus-tag for solubilization, thereby soluble and biologically active rpej-sgp-vii-amide ... | 2009 | 18722490 |
| upregulation of actin-like gene expression in giant freshwater prawns macrobrachium rosenbergii infected with metschnikowia bicuspidata. | the giant freshwater prawn macrobrachium rosenbergii is commercially cultured throughout the world including taiwan. from 1992 to 1995, taiwanese production decreased by approximately 50% due to disease. the yeast metschnikowia bicuspidata is considered to be one of the major causes of white muscle disease, but the molecular mechanism of its pathogenesis is not known. using rna differential display (dd) with muscle and hepatopancreatic tissue, we identified a 324 nucleotide (nt) message specific ... | 2005 | 16231645 |
| biochemical analysis and immunohistochemical examination of a gnrh-like immunoreactive peptide in the central nervous system of a decapod crustacean, the kuruma prawn (marsupenaeus japonicus). | we examined whether a gonadotropin-releasing hormone (gnrh)-like peptide exists in the central nervous system (cns) of the kuruma prawn, marsupenaeus japonicus, by reverse-phase high performance liquid chromatography (rphplc) combined with time-resolved fluoroimmunoassay (tr-fia) analysis and by immunohistochemistry. the displacement curve obtained for serially diluted extracts of the kuruma prawn brain paralleled the chicken gnrh-ii (cgnrh-ii) standard curve obtained by cgnrh-ii tr-fia using th ... | 2009 | 19968471 |
| identification of cdna encoding toll receptor, mjtoll gene from kuruma shrimp, marsupenaeus japonicus. | toll receptors are cell-surface receptors acting as pattern recognition receptors (prrs) that are involved in the signaling pathway for innate immunity activation and are genetically conserved from insects to mammals. tolls from penaeid shrimp are found in white leg shrimp litopenaeus vannamei (ltoll) and black tiger shrimp penaeus monodon (pmtoll). however, the molecular ligand-recognition patterns and identification of these penaeid toll classes remain unknown. here, we report cdna cloning of ... | 2008 | 18191582 |
| augmentation of protective immune responses against viral infection by oral administration of schizophyllan. | an oral administration of fungal polysaccharide schizophyllan has augmented protective immune responses to sendai virus infection in mice and the rodshaped dna virus of penaeus japonicus (rv-pj) infection in kuruma shrimps. when schizophyllan was administered orally at a dose of 50 or 100 mg/kg body weight per day, the survival rates after virus challenge were significantly higher than those of the control groups. high phagocytic activities were observed in the haemocytes of the schizophyllan-fe ... | 1997 | 18472856 |
| a novel focal adhesion kinase from marsupenaeus japonicus and its response to wssv infection. | focal adhesion kinase (fak) is a cytoplasmic protein-tyrosine kinase involved in integrin-mediated signal transduction which regulates multiple cell functions in mammalian cells. in contrast to the well document of fak in mammalian cells, the properties of fak in crustacean have not been reported yet and even none of their gene or protein sequences is known to date. here, we report for the first time the cloning of fak from marsupenaeus japonicus (designated as mjfak) and the identification of i ... | 2009 | 19013481 |
| identification and phylogenetic analysis on lipopolysaccharide and beta-1,3-glucan binding protein (lgbp) of kuruma shrimp marsupenaeus japonicus. | a lipopolysaccharide (lps) and beta-1,3-glucan binding protein (lgbp) gene was cloned from hemocytes of kuruma shrimp marsupenaeus japonicus by reverse-transcription polymerase chain reaction (rt-pcr), cloning and sequencing of overlapping pcr, and rapid amplification of cdna ends (race) method. the open reading frame (orf) of m. japonicus lgbp is 1062 bp and encodes a 354 amino acid (aa) sequence with a 23 aa signal peptide. the calculated molecular mass of the mature protein (331 aa) is 40.15 ... | 2008 | 18572243 |
| genomic organization of the translationally controlled tumor protein (tctp) gene from shrimp marsupenaeus japonicus. | the shrimp translationally controlled tumor protein (tctp) gene was previously found up-regulated in response to viral infection. in order to further understand the transcriptional regulation of tctp gene expression under viral challenge, the 5'-flanking promoter region of the tctp gene in shrimp marsupenaeus japonicus (mj-tctp) was cloned by using genomic walking method. the sequence shows that this region contains many important potential binding sites for transcription factors, such as activa ... | 2009 | 18587664 |
| molecular characterization of alanine racemase in the kuruma prawn marsupenaeus japonicus. | aquatic crustaceans and some bivalve mollusks are known to contain copious amounts of free d-alanine in their tissues. for the first time in the animal kingdom, we have isolated a cdna clone encoding alanine racemase from the muscle and hepatopancreas of the kuruma prawn marsupenaeus japonicus. the recombinant enzyme expressed in escherichia coli exhibited alanine recemase activity. the deduced amino-acid sequence showed only 23-31% identity to bacterial alanine racemases. however, the active si ... | 2009 | 19060308 |
| protection against white spot syndrome virus (wssv) infection in kuruma shrimp orally vaccinated with wssv rvp26 and rvp28. | white spot syndrome virus (wssv) is the causative agent of white spot disease (wsd), one of the most serious diseases affecting global shrimp farming. we compared wssv infection induction in kuruma shrimp marsupenaeus japonicus by oral, immersion, and intramuscular injection (im) exposure methods and evaluated the oral vaccine prepared from the recombinant wssv proteins rvp26 and rvp28. the 50% lethal doses (ld50) of wssv by oral, immersion, and im challenges were 10(-0.4), 109-4.4), and 10(-7.7 ... | 2008 | 19149371 |
| purification and properties of a beta-mannosidase from shrimp (penaeus japonicus) hepatopancreas. | a beta-mannosidase was purified ca 720-fold to homogeneity from penaeus japonicus, with a final spec. act. of 252 u/mg of protein. 2. by using sds-polyacrylamide gel electrophoresis, the monomers of shrimp beta-mannosidase were discovered to have mol. wts of 31,000 and those of human placental enzyme have similar mol. wts. 3.the shrimp beta-mannosidase has an isoelectric point (pi) of 5.6 +/- 0.1, and the human placental enzyme has an identical pi. both enzymes were sialyated. 4.the shrimp beta- ... | 1991 | 20506606 |
| characterization of phosphotyrosyl protein phosphatase fom the hepatopancreas of the shrimp penaeus japonicus (crustacea: decapoda). | phosphotyrosyl protein phosphatase, purified from the hepatopancreas of panaeus japonicus, is a monomeric enzyme with a relative mass (mr) of 28,000, as estimated by size-exclusion fplc on a superose 12. it has a hydrophobic domain and can be extracted with the detergent chaps.the specific activity of the purified enzyme was 9,800 units/mg of protein. the purified enzyme had an isoelectric point less than 4.6, and an optimal ph of 6.5 with either a synthetic peptide or autophosphorylated recepto ... | 1993 | 20509211 |
| studies on the transmission of wssv (white spot syndrome virus) in juvenile marsupenaeus japonicus via marine microalgae. | we studied the possible role that marine microalgae may play during the outbreaks of wss (white spot syndrome). in order to elucidate the possibility of marine microalgae carrying wssv (white spot syndrome virus), six marine microalgae (isochrysis galbana, skeletonema costatum, chlorella sp., heterosigma akashiwo, scrippsiella trochoidea, dunaliella salina) were co-cultured with adult marsupenaeus japonicus infected with wssv and were assayed daily by nested-pcr to study whether they could carry ... | 2007 | 17383676 |
| mj-dwd, a double wap domain-containing protein with antiviral relevance in marsupenaeus japonicus. | the mj-dwd (marsupenaeus japonicus' double-wap domains) gene was originally found up-regulated in virus-resistant shrimp m. japonicus by suppression subtractive hybridization (ssh). the full-length cdna of mj-dwd encodes a novel protein containing a kgd (lys-gly-asp) motif and double wap domains. performed by quantitative real-time pcr, the expression level of mj-dwd gene was consistently maintained at a high level in the newly prepared virus-resistant shrimp compared to the normal one. in addit ... | 2008 | 18974012 |
| isolation and cdna cloning of ovarian cortical rod protein in kuruma prawn marsupenaeus japonicus (crustacea: decapoda: penaeidae). | two cortical rod proteins having molecular weights of 28.6 kda and 30.5 kda were isolated from the mature ovary of marsupenaeus japonicus using gel filtration and reversed-phase hplc. analysis of the n-terminal amino acid sequence of the 28.6 kda molecule revealed that amino acid residues 1-21 corresponded to residues 9-29 of the 30.5 kda molecule. examination of homology using blast showed that 21 amino acids out of 29 residues of the 28.6 kda molecule, and 14 out of 29 residues of the 30.5 kda ... | 2004 | 15572862 |
| characterization of myosin light chain in shrimp hemocytic phagocytosis. | myosin light chain, a well-known cytoskeleton gene, regulates multiple processes that are involved in material transport, muscle shrink and cell division. however, its function in phagocytosis against invading pathogens in crustacean remains unknown. in this investigation, a myosin light chain gene was obtained from marsupenaeus japonicus shrimp. the full-length cdna of this gene was of 766 bp and an open reading frame (orf) of 462 bp encoding a polypeptide of 153 amino acids. the myosin light c ... | 2010 | 20691789 |
| immunomodulatory effect of plant-mixed feed in kuruma shrimp, marsupenaeus japonicus, and its protective efficacy against white spot syndrome virus infection. | | 2010 | 20873032 |
| scavenger receptor c mediates phagocytosis of white spot syndrome virus and restricts virus proliferation in shrimp. | scavenger receptors are an important class of pattern recognition receptors that play several important roles in host defense against pathogens. the class c scavenger receptors (srcs) have only been identified in a few invertebrates, and their role in the immune response against viruses is seldom studied. in this study, we firstly identified an src from kuruma shrimp, marsupenaeus japonicus, designated mjsrc, which was significantly upregulated after white spot syndrome virus (wssv) challenge at ... | 2016 | 28027319 |
| trbp and eif6 homologue in marsupenaeus japonicus play crucial roles in antiviral response. | plants and invertebrates can suppress viral infection through rna silencing, mediated by rna-induced silencing complex (risc). trans-activation response rna-binding protein (trbp), consisting of three double-stranded rna-binding domains, is a component of the risc. in our previous paper, a trbp homologue in fenneropenaeus chinensis (fc-trbp) was reported to directly bind to eukaryotic initiation factor 6 (fc-eif6). in this study, we further characterized the function of trbp and the involvement ... | 2012 | 22279564 |
| cleavage and gastrulation in the kuruma shrimp penaeus (marsupenaeus) japonicus (bate): a revised cell lineage and identification of a presumptive germ cell marker. | a previous study suggested that mesendoderm (me) cell arrest occurred at the 64-cell stage and a ring of eight presumptive naupliar mesoderm cells or crown cells surrounded the blastopore in the kuruma shrimp penaeus (marsupenaeus) japonicus. since this varied from the pattern observed in other penaeoidean shrimp, cleavage and gastrulation was re-examined in p. japonicus using the nucleic acid stain sytox green and confocal microscopy. in contrast to the earlier study, cleavage and gastrulation ... | 2010 | 20874712 |
| involvement of wssv-shrimp homologs in wssv infectivity in kuruma shrimp: marsupenaeus japonicus. | white spot syndrome virus (wssv) is pathogenic and specific to shrimp, and is capable of producing a persistent infection in the host. moreover, shrimp are capable of persistently carrying a single or multiple viruses, allowing them to survive for long periods with latent infections. in order to identify genes that are specially involved in the intricate wssv-shrimp association, we focused on homologs between the wssv and shrimp genomes. we here investigated whether homologous wssvorfs (wssvorf2 ... | 2010 | 20826185 |
| regulation of shrimp pjcaspase promoter activity by wssv vp38 and vp41b. | members of the caspase family play essential roles in apoptosis. in kuruma shrimp marsupenaeus japonicus the caspase gene (pjcaspase) was previously found dramatically up-regulated in viral-challenged and -resistant shrimp, suggesting that pjcaspase plays an important role in protecting host from viral infection. in order to further delineate the transcriptional regulation of pjcaspase in response to viral infection, the promoter activity was confirmed by fusing the 5'-flanking promoter region o ... | 2011 | 21362480 |
| deciphering the dna repair protein, rad23 from kuruma shrimp marsupenaeus japonicus: full-length cdna cloning and characterization. | aims: lesions of dna are removed by nucleotide excision repair (ner) process in the living systems. ner process-related host factors are believed to aid recovery steps during viral integration. here, we report identification and characterization of a dna repair molecule rad23 from kuruma shrimp marsupenaeus japonicus. methods and results: the full-length cdna of m. japonicus rad23 gene (mjrad23) has 1149 bp coding for a putative protein of 382 amino acids with a 5' untranslated region (utr) of ... | 2011 | 21535050 |
| characterization of two novel adp ribosylation factors from the shrimp marsupenaeus japonicus. | adp-ribosylation factors (arfs) that play an essential role in intracellular trafficking and organelle structure are small gtp-binding proteins, which have been identified recently to be involved in virus infection. however, little is known about the arfs and their relationships with viral infection in the economically important crustaceans to date. in the present study, two novel members of the arf family, designated as mjarf1 and mjarfn respectively, were cloned from the shrimp marsupenaeus ja ... | 2010 | 20713160 |
| molecular characterization and expression analysis of chitinase (fcchi-3) from chinese shrimp, fenneropenaeus chinensis. | complementary dna (cdna) and genomic dna, including flanking regions of the chitinase gene (fcchi-3) of fenneropenaeus chinensis, were cloned and sequenced. fcchi-3 was found to have 92.0 and 91.4% identity at the cdna level to that of litopenaeus vannamei and marsupenaeus japonicus, respectively. the predicted amino acid sequence had an overall similarity with a comparable region of l. vannamei (96.8%) and m. japonicus (93.4%). based on the cdna sequence, the genomic structure of the gene was c ... | 2010 | 19642017 |
| bioinformatic analyses of the publicly accessible crustacean expressed sequence tags (ests) reveal numerous novel neuropeptide-encoding precursor proteins, including ones from members of several little studied taxa. | ests have been generated for many crustacean species, providing an invaluable resource for peptide discovery in members of this arthropod subphylum. here, these data were mined for novel peptide-encoding transcripts, with the mature peptides encoded by them predicted using a combination of online peptide prediction programs and homology to known arthropod sequences. in total, 70 mature full-length/partial peptides representing members of 16 families/subfamilies were predicted, the vast majority ... | 2010 | 20064519 |
| isolation and identification of novel micrornas from marsupenaeus japonicus. | micrornas (mirnas) are a class of small noncoding rnas that function as regulators of gene expression. they play essential roles in various biological processes, such as development, differentiation and immune response. in this study, we identified 35 mirnas from marsupenaeus japonicus. among them, fifteen mirnas exhibited high homology to the known mirnas from other arthropods, while the rest might represent novel mirnas. we further showed a correlation of wssv infection and the expression leve ... | 2011 | 21658454 |
| molecular cloning and characterization of the nitric oxide synthase gene from kuruma shrimp, marsupenaeus japonicus. | nitric oxide (no) signaling is involved in many physiological processes in vertebrates and invertebrates. in crustaceans, nitric oxide synthase (nos) plays a significant role in the regulation of the nervous system and in innate immunity. here, we describe the entire cdna sequence (4616 bp) of the kuruma shrimp marsupenaeus japonicus nos (mj nos) generated using the reverse transcriptase-polymerase chain reaction (rt-pcr) and 5'- and 3'- rapid amplification pcrs of cdna ends from brain and gill ... | 2010 | 20109558 |
| cloning and characterization of three novel wssv recognizing lectins from shrimp marsupenaeus japonicus. | c-type lectins (ctls) acting as pattern recognition receptors play essential roles in shrimp innate immune responses. using wssv envelope proteins (vp26, vp28, and vp281) to screen a phage display library of marsupenaeus japonicus, three lectins (termed as mjleca, mjlecb, and mjlecc) were found to interact with wssv. sequence analysis revealed that these mjlecs shared low similarities with each other. phylogenetic analysis indicated mjleca and mjlecb are likely to belong to the same lectin sub-f ... | 2010 | 20045060 |
| molecular cloning and characterization of alpha 2-macroglobulin (alpha2-m) from the haemocytes of chinese mitten crab eriocheir sinensis. | alpha 2-macroglobulin (alpha2-m) is a large protein in blood and is able to inactivate a variety of proteinases in invertebrates and vertebrates. the alpha2-m gene was obtained from the chinese mitten crab, eriocheir sinensis, by the reverse transcript-polymerase chain reaction (rt-pcr) and the rapid amplification of cdna ends (race). the alpha2-m cdna of e. sinensis contained 4851 bp with an open reading frame of 4371 bp, a 70 bp 5'-untranslated region, a 410 bp 3'-untranslated region, and thre ... | 2010 | 20394825 |
| class b scavenger receptor, croquemort from kuruma shrimp marsupenaeus japonicus: molecular cloning and characterization. | the scavenger receptor, croquemort is a member of the cd36 superfamily comprising transmembrane proteins involved in the recognition of polyanionic ligands. various researchers have proved that members of the cd36 superfamily are involved in immunity and developmental processes. in the present study, we report a cdna encoding the kuruma shrimp, marsupenaeus japonicus croquemort scavenger receptor (mjscrbq) obtained from a cdna library of lymphoid organ by race amplification. the full-length cdna ... | 2011 | 21324353 |
| a novel gene of tumor necrosis factor ligand superfamily from kuruma shrimp, marsupenaeus japonicus. | a tumor necrosis factor (tnf) gene has been isolated and characterized in kuruma shrimp, marsupenaeus japonicus, providing the first conclusive evidence for the existence of the tnf ligand in shrimp. the kuruma shrimp tnf (mjtnf) cdna was composed of 1868 bp with a 262 bp 5'-untranslated region (utr) and a 220 bp 3'-utr, which was translated into a protein of 462 amino acid residues that included a predicted transmembrane domain of 23 amino acid residues (trp20-val42) and the tnf family signatur ... | 2010 | 20045063 |
| gastrointestinal digestion and absorption of pen j 1, a major allergen from kuruma prawn, penaeus japonicus. | tropomyosin had been identified as a major allergen in shrimp. the digestion and absorption of tropomyosin (pen j 1) from kuruma prawn were investigated by ex vivo, in vitro, and in vivo techniques in order to elucidate the relationship between the allergenicity of the allergen and its gastrointestinal behavior. pen j 1 transported the caco-2 monolayer in a dose-dependent manner, and also enhanced the permeability of lucifer yellow, a marker of paracellular transportation, at high concentrations ... | 2011 | 21737942 |
| Molecular cloning, characterization and expression analysis of the tumor necrosis factor (TNF) superfamily gene, TNF receptor superfamily gene and lipopolysaccharide-induced TNF-+¦ factor (LITAF) gene from Litopenaeus vannamei. | In vertebrates, the tumor necrosis factor (TNF)-receptor (TNFR) system participates in diverse physiological and pathological events, such as inflammation and protective immune responses to microbial infections. There are few reports about the role of the invertebrate TNF-TNFR system in immune responses. Here, we isolated and characterized the TNF superfamily (LvTNFSF) gene, TNFR superfamily (LvTNFRSF) gene and lipopolysaccharide-induced TNF-+¦ factor (LvLITAF) gene from Litopenaeus vannamei. Lv ... | 2011 | 21736897 |
| anti-lipopolysaccharide factor evokes indirect killing of virulent bacteria in kuruma prawn. | aims: anti-lipopolysaccharide factor (alf) is an antimicrobial peptide (amp) and a key effector molecule of the innate immune system in crustaceans. however, little is known about the role of its indirect killing against bacteria. the possible regulatory role of this peptide (m-alf) in kuruma prawns, marsupenaeus japonicus, was investigated. | 2011 | 21753127 |
| thermodynamic characterization of muscle tropomyosins from marine invertebrates. | structural properties of invertebrate muscle tropomyosin (tm) have not been characterized in detail to date. tms were thus purified from the mantle muscle of japanese common squid todarodes pacificus, the foot muscle of tokobushi abalone haliotis diversicolor and the tail muscle of kuruma prawn marsupenaeus japonicus, and investigated for their thermodynamic properties by circular dichroism (cd) spectrometry and differential scanning calorimetry (dsc). from the cd spectrometry data, the apparent ... | 2011 | 21722747 |
| Functional Analysis of C-type Lysozyme in Penaeid Shrimp. | Lysozyme is an enzyme that cleaves the ß-1,4-glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine in peptidoglycan, leading to bacterial lysis. Recently, lysozyme has been found to have anti-HIV and anti-cancer properties in mammals. However, most functional analyses were done in vitro using purified or recombinant lysozyme protein. Here, we used RNA interference to silence c-type lysozyme expression in penaeid shrimp, Marsupenaeus japonicus, to analyze the function of lysozy ... | 2011 | 22069322 |
| natural host-range and experimental transmission of laem-singh virus (lsnv). | slow growth caused by viral diseases has become a major constraint in shrimp aquaculture. laem-singh virus (lsnv), a positive-sense single-stranded rna (ssrna) virus, has been identified in penaeus monodon showing slow growth syndrome. to examine the host-range and transmission modes of the virus, 6 species of penaeid shrimp of varying life stages, sourced from the wild and from farms, as well as juvenile mud crabs scylla serrata, were screened using rt-nested pcr. lsnv was detected in p. monodo ... | 2011 | 21991662 |
| Crystallization and preliminary X-ray analysis of crustacean hyperglycaemic hormone from the kuruma prawn Marsupenaeus japonicus in its weakly active precursor form. | Crustacean hyperglycaemic hormone (CHH) plays a pivotal role in the regulation of glucose metabolism in crustaceans. Pej-SGP-I, one of the six known CHHs in the kuruma prawn Marsupenaeus japonicus, was heterologously expressed in Escherichia coli as an N-terminally His-tagged and Nus-tagged protein in its weakly active precursor form, Pej-SGP-I-Gly, which has an extra glycine residue at the C-terminus. The recombinant peptide was subjected to affinity purification, tag removal, further purificat ... | 2011 | 22139173 |
| Identification and cloning of a transglutaminase from giant freshwater prawn, Macrobrachium rosenbergii, and its transcription during pathogen infection and moulting. | Complementary (c)DNA encoding transglutaminase (TG) messenger (m)RNA of the giant freshwater prawn, Macrobrachium rosenbergii, was cloned from haemocytes by a reverse-transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) using oligonucleotide primers based on the TG sequence of the horseshoe crab, Tachypleus tridentatus; tiger shrimp, Penaeus monodon; kuruma shrimp, Marsupenaeus japonicus; and crayfish, Pacifastacus leniusculus. The 2722-bp cDNA contained a ... | 2011 | 21854853 |
| hyper-expansion of large dna segments in the genome of kuruma shrimp, marsupenaeus japonicus. | higher crustaceans (class malacostraca) represent the most species-rich and morphologically diverse group of non-insect arthropods and many of its members are commercially important. although the crustacean dna sequence information is growing exponentially, little is known about the genome organization of malacostraca. here, we constructed a bacterial artificial chromosome (bac) library and performed bac-end sequencing to provide genomic information for kuruma shrimp (marsupenaeus japonicus), on ... | 2010 | 20187930 |
| Molecular characterization of a Ran isoform gene up-regulated in shrimp immunity. | Diseases caused by viruses are the greatest challenge to worldwide shrimp aquaculture. Ran gene was an important antiviral gene identified from shrimp and its mRNA level was up-regulated in response to viral infection. In this investigation, a Ran isoform gene (named Ran-iso) cDNA was cloned from shrimp, Marsupenaeus japonicus. The full-length cDNA of Ran-iso was 1286bp, including a 5'-terminal untranslated region (UTR) of 272bp, 3'-terminal UTR of 366bp and an open reading frame (ORF) of 648bp ... | 2011 | 22192911 |
| molecular cloning, characterization, and expression analysis of two different types of lectins from the oriental river prawn, macrobrachium nipponense. | lectins, which are widely expressed in invertebrates, play important roles in many biological processes, including protein trafficking, cell signaling, pathogen recognition, as effector molecules, and so on (wang and wang, 2013). this study identified one novel m-type lectin and one l-type lectin, designated as mnmtl1 and mnltl1, from the oriental river prawn macrobrachium nipponense. the full-length cdna of mnmtl1 was 2064 bp with a 1761 bp orf encoding a putative protein of 586 deduced amino a ... | 2015 | 25929240 |
| Alpha2-macroglobulin from an Atlantic shrimp: biochemical characterization, sub-cellular localization and gene expression upon fungal challenge. | In this study, we report on the isolation and characterization of an alpha2-macroglobulin (a2M) from the plasma of the pink shrimp Farfantepenaeus paulensis, its sub-cellular localization and transcriptional changes after infection by fungi. The molecular mass of the a2M was estimated at 389 kDa by gel filtration and 197 kDa by SDS-PAGE, under reducing conditions, suggesting that a2M from F. paulensis consists of two identical sub-units, covalently linked by disulphide bonds. The N-terminal amin ... | 2011 | 21888978 |
| contribution of the argonaute-1 isoforms to invertebrate antiviral defense. | argonaute (ago) protein, the central component of the rna interference (rnai) pathway, plays important roles in host innate antiviral immunity. most organisms harbor a large number of different ago proteins and isoforms; however, the roles of ago isoforms in immune defense against pathogens remain unclear. in the present study, three argonaute-1 (ago1) isoforms, termed ago1a, ago1b, and ago1c, were found in marsupenaeus japonicus shrimp. quantitative real-time pcr (polymerase chain reaction) rev ... | 2012 | 23209784 |
| extracellular trap formation in kuruma shrimp (marsupenaeus japonicus) hemocytes is coupled with c-type lysozyme. | in invertebrates, hemocytes play an important role in immune responses. recently, a novel form of innate immune mechanism called extracellular traps (ets) was identified in shrimps, where dna and antimicrobial peptides form complex structure to entrap the invading microbes. in this study, we detected the formation of ets from hemocytes of kuruma shrimp in response to various stimulations, including phorbol myristate acetate (pma), lipopolysaccharide (lps), peptidoglycan (pgn) and escherichia col ... | 2016 | 27012393 |
| four crustins involved in antibacterial responses in marsupenaeus japonicus. | crustins are a family of cationic, cysteine-rich antimicrobial peptides with a whey acidic protein (wap) domain in the c-terminal. they have diverse functions in antimicrobial immune responses. four groups of crustins (crustins i, ii, iii, and iv) have been identified in crustaceans, but type i crustins have not been reported in penaeid shrimp until now. in this study, we identified four crustins in kuruma shrimp marsupenaeus japonicus, and named them mjcrus i-2, 3, 4 and 5. these four crustins ... | 2015 | 25583545 |
| transcriptome-wide identification and characterization of the procambarus clarkii micrornas potentially related to immunity against spiroplasma eriocheiris infection. | we used the illumina/solexa deep sequencing technology to sequence two small rna libraries prepared from hemocytes of procambarus clarkii under normal and infection conditions. the high-throughput sequencing approach resulted in approximately 12,801,827 and 8,410,455 raw reads corresponding to 10,949,754 and 6,648,161 high-quality mappable reads for the normal and infected hemocyte samples, respectively. bioinformatic analyses identified 195 unique mirnas, including 30 that are conserved in crus ... | 2013 | 23747834 |
| leucine-rich repeats containing protein functions in the antibacterial immune reaction in stomach of kuruma shrimp marsupenaeus japonicus. | leucine rich repeat (lrr) motif exists in many immune receptors of animals and plants. most lrr containing (lrrc) proteins are involved in protein-ligand and protein-protein interaction, but the exact functions of most lrrc proteins were not well-studied. in this study, an lrrc protein was identified from kuruma shrimp marsupenaeus japonicus, and named as mjlrrc1. mjlrrc1 was consistently expressed in different tissues of normal shrimp with higher expression in gills and stomach. at the transcri ... | 2017 | 28027987 |
| A novel protein with a fibrinogen-like domain involved in the innate immune response of Marsupenaeus japonicus. | Fibrinogen-related proteins play important roles in innate immunity. We isolated a fibrinogen-related protein gene (MjFREP1) in kuruma shrimp Marsupenaeus japonicus. MjFREP1 encoded a protein of 270 amino acids, including a 223 amino acid fibrinogen-like domain. Quantitative real-time polymerase chain reaction analysis shows that MjFREP1 is mainly expressed in the gills and the expression is significantly upregulated by Vibrio anguillarum, Staphylococcus aureus, or white spot syndrome virus (WSS ... | 2012 | 22142703 |
| Molecular cloning and characterization of the NADPH oxidase from the kuruma shrimp, Marsupenaeus japonicus: Early gene up-regulation after Vibrio penaeicida and poly(I:C) stimulations in vitro. | Free radicals such as nitric oxide (NO) and reactive oxygen species (ROS) are involved in many physiological processes. In humans, there are 5 homologs of nicotinamide adenine dinucleotide phosphate (NADPH) oxidases (Noxes) that generate superoxide (O(2)(-)), which can dismute to produce ROS, and play significant roles in innate immunity and cell proliferation. Though Noxes have been identified in vertebrates (humans and fishes) and some insects, there are very few reports investigating Noxes in ... | 2011 | 22133377 |
| differential expression of micrornas in shrimp marsupenaeus japonicus in response to vibrio alginolyticus infection. | till date numerous micrornas (mirnas) have been discovered from various organisms, including mammals, plants, insects, nematodes and viruses. they are known to have antiviral functions in crustaceans such as shrimp marsupenaeus japonicas. however, little is known about the role of mirnas against bacterial infection in this shrimp caused by vibrio alginolyticus. we performed small rna sequencing to characterize the differentially expressed micrornas in v. alginolyticus challenged shrimp, in compa ... | 2016 | 26483347 |
| molecular cloning and characterization of the lipopolysaccharide and β-1,3-glucan binding protein from oriental river prawn, macrobrachium nipponense. | the lipopolysaccharide and β-1,3-glucan binding protein (lgbp), one of the pattern recognition proteins, plays an important role in the innate immune response of invertebrates. a 1,506 bp full-length cdna of a lgbp gene was cloned and characterized from the oriental river prawn macrobrachium nipponense (named as mnlgbp). analysis of the nucleotide sequence revealed that the cdna clone has an open reading frame of 1,119 bp, encoding a protein of 372 amino acids including a 21-aa signal peptide. t ... | 2014 | 24584659 |
| dual oxidases participate in the regulation of intestinal microbiotic homeostasis in the kuruma shrimp marsupenaeus japonicus. | the metazoan gut lumen harbors numerous microbial communities. tolerance for high bacterial counts and maintenance of microbiota homeostasis remain insufficiently studied. in this study, we identified a novel dual oxidase (mjduox2) involved in reactive oxygen species (ros) production in the kuruma shrimp marsupenaeus japonicus. mjduox2 is a transmembrane protein with an n-signal peptide region (19 aa) and a peroxidase homology domain (phd, 554 aa) in the extracellular region; seven transmembrane ... | 2016 | 26845611 |
| molecular cloning and characterization of a toll receptor gene from macrobrachium rosenbergii. | toll receptors are cell surface molecules acting as pattern recognition receptors (prrs) that have been implicated in the signaling pathway of innate immune responses. in this study, the full-length cdna of a toll receptor gene of macrobrachium rosenbergii, designated mrtoll, was successfully isolated using designed degenerate primers and the rapid amplification of cdna ends (race). the mrtoll gene sequence contained an open reading frame (orf) of 2799 nucleotides encoding a protein of 932 amino ... | 2014 | 24398262 |
| a new group of anti-lipopolysaccharide factors from marsupenaeus japonicus functions in antibacterial response. | anti-lipopolysaccharide factors (alfs) are a group of critical effector molecules with a broad spectrum of antimicrobial activities in crustaceans. four groups of alfs (a, b, c, and d) have been identified in peneaid shrimp. in the study, we identified a new group of alfs (designated as mjalf-e) from marsupenaeus japonicus. this new group (group e) included mjalf-e1 and e2. mjalf-e1 was highly expressed in hemocytes, heart, and intestine, whereas e2 was highly expressed in gills, stomach, and in ... | 2015 | 25218642 |
| calnexin functions in antibacterial immunity of marsupenaeus japonicus. | calnexin (cnx) is an endoplasmic reticulum membrane-bound lectin chaperone that comprises a dedicated maturation system with another lectin chaperone calreticulin (crt). this maturation system is known as the cnx/crt cycle. the main functions of cnx are ca(2+) storage, glycoprotein folding, and quality control of synthesis. recent studies have shown that cnx is important in phagocytosis and in optimizing dendritic cell immunity. however, the functions of cnx in invertebrate innate immunity remai ... | 2014 | 24858031 |
| enzymatic elucidation of haemocyanin from kuruma shrimp marsupenaeus japonicus and its molecular recognition mechanism towards pathogens. | haemocyanin is an important non-specific immune protein present in the hemolymph of invertebrates, which have the ability to recognize the microbial pathogens and trigger the innate immune system. in this study, we isolated and purified the haemocyanin using gel filtration chromatography and investigated its microbial recognition mechanism against the invading pathogens. kuruma shrimp marsupenaeus japonicus haemocyanin showed the single band with a molecular weight of 76 kda on sds-page and its ... | 2015 | 25204648 |
| protection of shrimp against white spot syndrome virus (wssv) with β-1,3-d: -glucan-encapsulated vp28-sirna particles. | white spot syndrome virus (wssv) is a major shrimp viral pathogen responsible for large economic losses to shrimp aquaculture all over the world. the rnai mediated by sirna contributes a new strategy to control this viral disease. however, the efficient approach to deliver the sirna into shrimp remains to be addressed. in this investigation, an antiviral vp28-sirna was encapsulated in β-1,3-d: -glucan, and then the β-1,3-d: -glucan-encapsulated vp28-sirna particles (gerps) were delivered into ma ... | 2011 | 21590271 |
| suppressor of cytokine signaling 2 (socs2) negatively regulates the expression of antimicrobial peptides by affecting the stat transcriptional activity in shrimp marsupenaeus japonicus. | the suppressor of cytokine signaling (socs) family is a kind of negative regulators in the janus kinase/signal transducer and activator of transcription (jak/stat) pathway in mammals and drosophila. in kuruma shrimp, marsupenaeus japonicus, socs2 is identified and its expression can be stimulated by peptidoglycan and polycytidylic acid. however, if socs2 participates in regulating jak/stat pathway in shrimp still needs further study. in this study, socs2 with src homology 2 domain and socs box w ... | 2016 | 27492125 |
| characterization of a 2-cys peroxiredoxin iv in marsupenaeus japonicus (kuruma shrimp) and its role in the anti-viral immunity. | accumulating evidence suggests that peroxiredoxins (prx) are key molecules in the pathogenesis of various infectious diseases and are potential therapeutic targets for major diseases such as cancers. in this study, we report a peroxiredoxin iv (prx iv) in marsupenaeus japonicus, designated as mjprx iv, which exhibited peroxidase activity and participated in the anti-white spot syndrome virus (wssv) immune response. mjprx iv is a 245-amino acid polypeptide with a predicted 19-amino acid signal pe ... | 2013 | 24056278 |
| a galectin from the kuruma shrimp (marsupenaeus japonicus) functions as an opsonin and promotes bacterial clearance from hemolymph. | galectins are a lectin family characterized by a conserved sequence motif in the carbohydrate recognition domain, which preferential binds to galactosyl moieties. however, few studies about the biological roles of galectins in invertebrates have been reported except for the galectin (cvgal1) from the eastern oyster crassostrea virginica. furthermore, galectins have been described in only a few crustacean species, and no functional studies have been reported so far. in this study, we identified a ... | 2014 | 24618590 |
| a recombinant molt-inhibiting hormone of the kuruma prawn has a similar secondary structure to a native hormone: determination of disulfide bond arrangement and measurements of circular dichroism spectra. | in crustaceans, molt-inhibiting hormone (mih) is presumed to regulate molting through suppressing synthesis and/or secretion of ecdysteroids by the y-organ. recently, a recombinant mih of the kuruma prawn penaeus japonicus was produced in e. coli. to approximate the secondary structure of native and recombinant mih of p. japonicus containing six cysteine residues, the arrangements of disulfide bridges in both mihs were determined by characterizing their enzymatic digests, and their circular dich ... | 2001 | 11577724 |
| involvement of a lysm and putative peptidoglycan-binding domain-containing protein in the antibacterial immune response of kuruma shrimp marsupenaeus japonicus. | lysin motif (lysm) is a peptidoglycan and chitin-binding motif with multiple functions in bacteria, plants, and animals. in this study, a novel lysm and putative peptidoglycan-binding domain-containing protein was cloned from kuruma shrimp (marsupenaeus japonicus) and named as mjlpbp. the cdna of mjlpbp contained 1010 nucleotides with an open reading frame of 834 nucleotides encoding a protein of 277 amino acid residues. the deduced protein contained a lysin motif and a transmembrane region, wit ... | 2016 | 27142936 |
| isolation of lactic acid bacteria from kuruma shrimp (marsupenaeus japonicus) intestine and assessment of immunomodulatory role of a selected strain as probiotic. | fifty-one lactic acid bacteria (lab) strains were isolated and identified based on 16s ribosomal dna sequence from the intestinal tracts of 142 kuruma shrimps (marsupenaeus japonicus) collected from kanmon strait, fukuoka and tachibana bay, nagasaki, japan. cellular immunomodulatory function of 51 isolated lab strains was assessed by measuring the level of interferon (ifn)-γ induction in mouse spleen cell culture. the strain lactococcus lactis d1813 exhibited the highest amount of ifn-γ producti ... | 2014 | 24045901 |
| a novel crustin from marsupenaeus japonicus promotes hemocyte phagocytosis. | crustins are cationic cysteine-rich antimicrobial peptides (amps) that contain multiple domains (glycine-rich, cysteine-rich, or proline-rich) at the n-terminus and whey acidic protein (wap) domains at the c-terminus. crustins have multiple functions, including protease inhibition and antimicrobial activity. other functions of crustins need to be clarified. in this study, a novel crustin with a cysteine-rich region, and a single wap domain, belonging to type i crustins, was identified in marsupe ... | 2015 | 25479014 |
| syntenin is involved in the bacteria clearance response of kuruma shrimp (marsupenaeus japonicus). | syntenin is a multifunctional cytosolic adaptor protein that contributes to cell migration, proliferation, attachment, and apoptosis, as well as immune response to virus, in vertebrates. however, the functions of syntenin in the antibacterial response of invertebrates remain unclear. in this study, we identified a syntenin-like gene (mjsyn) from the kuruma shrimp (marsupenaeus japonicus) and detected its function in the antibacterial immunity of shrimp. the full-length mjsyn was 1223 bp with a 9 ... | 2015 | 25731918 |
| a fibrinogen-related protein (frep) is involved in the antibacterial immunity of marsupenaeus japonicus. | fibrinogen-related proteins (freps) in invertebrates have important functions in innate immunity. in this study, the cdna of frep was identified from the kuruma shrimp marsupenaeus japonicus (mjfrep2). the full-length cdna of mjfrep2 is 1138 bp with an open reading frame of 954 bp that encodes a 317-amino acid protein comprising a signal peptide and a fibrinogen-like domain. mjfrep2 could be detected in hemocytes, heart, hepatopancreas, gills, stomach, and intestines. mjfrep2 could also be upreg ... | 2014 | 24830772 |
| role of evolutionarily conserved signaling intermediate in toll pathways (ecsit) in the antibacterial immunity of marsupenaeus japonicus. | the toll/toll-like receptor (tlr) signaling pathway has an important role in the innate immunity of animals. evolutionarily conserved signaling intermediate in toll pathways (ecsit) is a protein that functions as an adaptor protein for the toll/tlr and bone morphogenetic protein signaling pathways. ecsit is also a key component in the macrophage bactericidal activity of mammals. however, the function of ecsit in crustaceans remains unclear. in this study, we cloned and identified a functional ec ... | 2014 | 24796866 |
| identification and cloning of the second type transglutaminase from litopenaeus vannamei, and its transcription following pathogen infection and in relation to the haemolymph coagulation. | complementary (c)dna encoding transglutaminaseii (tgii) messenger (m)rna of white shrimp, litopenaeus vannamei, was cloned from haemocytes by a reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends (race) using oligonucleotide primers based on the tg sequence of the horseshoe crab, tachypleus tridentatus (accession no.: baa02134), tiger shrimp, penaeus monodon (aav49005; aao33455), kuruma shrimp, marsupenaeus japonicus (bad36808) and pacifastacus leniuscul ... | 2013 | 24036331 |
| β-arrestin 1's interaction with tc45 attenuates stat signaling by dephosphorylating stat to inhibit antimicrobial peptide expression. | impaired phosphatase activity leads to the persistent activation of signal transducers and activators of transcription (stat). in mammals, stat family members are often phosphorylated or dephosphorylated by the same enzymes. to date, only one stat similar to mammalian stat5a/b has been found in crustaceans and there have been few studies in stat signal regulation in crustaceans. here, we report that β-arrestin1 interacts with tc45 (45-kda form of t cell protein tyrosine phosphatase) in the nucle ... | 2016 | 27782165 |
| characterization of argonaute2 gene from black tiger shrimp (penaeus monodon) and its responses to immune challenges. | argonaute2 binds to a short guide rna (microrna or short interfering rna) and guides rnas direct risc to complementary mrnas that are targets for risc-mediated gene silencing. here we identified and characterized argonaute2 from black tiger shrimp penaeus monodon (designated as pmago2). the full-length cdna of pmago2 contained a 5' untranslated region (utr) of 106 bp, an open reading frame (orf) of 2616 bp and a 3' utr of 123 bp. the predicted pmago2 protein is 99.4 kda with the theoretical isoe ... | 2014 | 24262300 |
| a novel method of real-time reverse-transcription loop-mediated isothermal amplification developed for rapid and quantitative detection of a new genotype (yhv-8) of yellow head virus. | a new genotype of yellow head virus (yhv), designated as yhv-8, was found in farmed shrimp fenneropenaeus chinensis suffering suspectedly from ems/ahpns (early mortality disease/acute hepatopancreatic necrosis disease) in china in 2012. in this study, a one-step, real-time reverse-transcription loop-mediated isothermal amplification (rrt-lamp) assay was developed for better detection of both genotypes of yhv-1 and yhv-8. a set of six specific primers was successfully designed targeting a conserv ... | 2016 | 27221155 |
| role of marsupenaeus japonicus crustin-like peptide against vibrio penaeicida and white spot syndrome virus infection. | crustins are important amp that has been identified in crustaceans. in this study, the role of marsupenaeus japonicus crustin-like peptide (mjcrs) was examined in vivo by rna interference (rnai) using double-stranded rna (dsrna). tissue expression analysis revealed that mjcrs transcripts are expressed in different tissues tested with the highest expression observed in hemocytes. treatment with double-stranded rna specific to mjcrs led to a significant reduction of mjcrs transcripts within the he ... | 2014 | 24929027 |
| vibrio zhuhaiensis sp. nov., isolated from a japanese prawn (marsupenaeus japonicus). | a gram-negative, oxidase-positive, facultatively anaerobic bacterium, designated strain e20121, was isolated from the digestive tract of a japanese prawn (marsupenaeus japonicus) collected from the coastal sea water area of zhuhai, guangdong province, china. the new isolate was determined to be closely related to vibrio ponticus dsm 16217(t), having 97.6 % 16s rrna gene sequence similarity. phylogenetic analysis based on reca, pyrh and rpoa also showed low levels of sequence similarities (72.6-9 ... | 2013 | 23338602 |
| relationship between essential fatty acid requirements of aquatic animals and the capacity for bioconversion of linolenic acid to highly unsaturated fatty acids. | 1. [1-14c]linolenic acid was injected into the rainbow trout, salmo gairdnerii, ayu, plecoglossus altivelis, eel, anguilla japonica, red sea bream, chrysophrys major, rockfish, sebastiscus marmoratus, globefish, fugu rubripes rubripes and prawn, penaeus japonicus (molting stage d"1-d2), and the bioconversion of linolenic acid (18:3 omega 3) to highly unsaturated fatty acids such as eicosapentaenoic (20:5 omega 3) and docosahexaenoic (22:6 omega 3) acids was investigated. 2. linolenic acid was co ... | 1979 | 233802 |
| carbon dioxide and methane fluxes from feeding and no-feeding mariculture ponds. | the co2 and ch4 fluxes at the water-air interface were measured in shrimp (marsupenaeus japonicus) monoculture ponds (s) with feed supply and shrimp-sea cucumber (apostichopus japonicus) polyculture ponds (ss) without feed supply. during farming seasons of the whole year, cumulated co2-c fluxes were -5.69 g m(-2) (s) and 11.23 g m(-2) (ss), respectively. the cumulated co2 emissions from s and ss did not differ significantly. the cumulated ch4-c emissions from s (0.57 g m(-2)) were significantly ... | 2016 | 26967535 |
| a single whey acidic protein domain containing protein (swd) inhibits bacteria invasion and dissemination in shrimp marsupenaeus japonicus. | the single whey acidic protein (wap) domain containing proteins (swds) in crustacean belong to type iii crustins and have antiprotease activities and/or antimicrobial activities. their functions in vivo in crustacean immunity need to be clarify. in this study, a new single wap domain containing protein (swd) was obtained from marsupenaeus japonicus, designated as mjswd. the full-length cdna of mjswd was 522 bp.the open reading frame of mjswd encoded a protein of 79 amino acids, with a 24 amino a ... | 2013 | 23665548 |
| identification of novel copper/zinc superoxide dismutase (cu/znsod) genes in kuruma shrimp marsupenaeus japonicus. | superoxide dismutases (sods) protect cells from superoxides, but in invertebrates, they also have role in the innate immune system. in this study, the genes for five isoforms of copper/zinc superoxide dismutase (mjcu/znsod) gene were identified and sequenced in kuruma shrimp, marsupenaeus japonicus. the coding parts of the genes ranged from 516 to 585 bp in length and encoded from 172 to 194 amino acids. structure, phylogenetic and blast analyses indicated that mjcu/znsod isoform_3 and _5 belong ... | 2014 | 25107696 |
| characterization of a kunitz-type protease inhibitor (mjkupi) reveals the involvement of mjkupi positive hemocytes in the immune responses of kuruma shrimp marsupenaeus japonicus. | serine proteases and their inhibitors play vital roles in biological processes. serine protease inhibitors, including kunitz-type protease inhibitors play important roles not only in physiological process (i.e. blood clotting and fibrinolysis) but also in immune responses. in this study, we characterized a kunitz-type protease inhibitor, designated mjkupi, from kuruma shrimp marsupenaeus japonicus. an expression profile showed that mjkupi was mainly expressed in hemocytes. immunostaining reveale ... | 2016 | 27255219 |
| antibacterial activity of serine protease inhibitor 1 from kuruma shrimp marsupenaeus japonicus. | serine protease inhibitors (serpins) are a large family of protease inhibitors involved in many critical biological processes such as blood coagulation, fibrinolysis, programmed cell death, development, and innate immunity. we identified mjserp1, a serpin in the kuruma shrimp marsupenaeus japonicus. the mjserp1 cdna has a 1239 bp open reading frame (orf) that encodes a 412-amino acid protein with a 23 aa signal peptide and a classic serpin domain. mjserp1 has a calculated molecular mass of 46.3 ... | 2014 | 24412215 |
| computational analysis of molt-inhibiting hormone from selected crustaceans. | molt-inhibiting hormone (mih) is a principal endocrine hormone regulating the growth in crustaceans. in total, nine mih peptide sequences representing members of the family penaeidae (penaeus monodon, litopenaeus vannamei, marsupenaeus japonicus), portunidae (portunus trituberculatus, charybdis japonica, charybdis feriata), cambaridae (procambarus bouvieri), parastacidae (cherax quadricarinatus) and varunidae (eriocheir sinensis) were selected for our study. in order to develop a structure based ... | 2013 | 24041714 |
| characterization and expression pattern of p53 during spermatogenesis in the chinese mitten crab eriocheir sinensis. | p53, as a "guardian of the genome", plays an important role in cell cycle arrest, apoptosis, dna repair and inhibition of angiogenesis in different tissues including testis. p53 gene and its protein perform many essential roles for mammalian spermatogenesis. to explore its functions during spermatogenesis in eriocheir sinensis, we have cloned and sequenced the cdna (1,218 bp) of p53 from the testis by degenerating primer pcr and rapid-amplification of cdna ends. the protein alignment of p53 show ... | 2013 | 23065235 |
| a new nodavirus is associated with covert mortality disease of shrimp. | a new nodavirus, named covert mortality nodavirus (cmnv), is associated with covert mortality disease of shrimp which has caused serious loss in china since 2009. histopathological examination of shrimp suffering the disease revealed coagulative necrosis of striated muscle similar to typical histopathology features of infectious myonecrosis virus (imnv), penaeus vannamei nodavirus (pvnv) and macrobrachium rosenbergii nodavirus (mrnv). however, shrimp suffering this disease tested negative for im ... | 2014 | 25205685 |
| a homologue gene of β-catenin participates in the development of shrimps and immune response to bacteria and viruses. | β-catenin is a multifunctional protein that is involved in many physiological processes, including development, cell proliferation, cell migration, and apoptosis. however, the function of β-catenin in crustacean is unknown. in this study, the first shrimp homologous gene of β-catenin in marsupenaeus japonicus (i.e., mjβ-catenin) was identified and characterized. the full-length of the complementary dna of mjβ-catenin is 3130 bp, including a 2463 bp open reading frame that encodes 821 amino acid. ... | 2015 | 26334791 |
| the crustin-like peptide plays opposite role in shrimp immune response to vibrio alginolyticus and white spot syndrome virus (wssv) infection. | crustin is an antimicrobial peptide (amp) that plays a key role in innate immunity of crustaceans. in this study, we cloned the entire 660 bp crustin-like sequence with a 507 bp open reading frame encoding a 168 amino acid from marsupenaeus japonicus. the crustin-like gene was primarily expressed in gills and over-expressed in shrimp hemocytes after challenge with wssv or vibrio alginolyticus. after knockdown crustin-like gene using specific double-stranded rna (cru-dsrna), imd, rab7, l-lectin, ... | 2017 | 28546026 |
| molecular characterization of a p38 mapk from litopenaeus vannamei and its expression during the molt cycle and following pathogen infection. | the p38 mitogen-activated protein kinase (mapk), a serine/threonine-specific protein kinase, has been reported to be involved in innate immunity, development and muscle differentiation. to explore the function of p38 in shrimp, partial cdna sequence of p38 in litopenaeus vannamei (designated as lv-p38) was characterized and the expression of lv-p38 in hepatopancreas of the shrimp after being infected with vibrio parahaemolyticus and in muscle of the shrimp at different molt stages was detected b ... | 2013 | 23707783 |
| death associated protein 1 (dap 1) positively regulates virus replication and apoptosis of hemocytes in shrimp marsupenaeus japonicus. | death-associated protein 1 (dap1) is a small proline-rich cytoplasmic protein that functions both in the apoptosis and autophage process of mammalian and in the clinical cancer of human. however, little knowledge is known about the homologue gene of dap1 and its roles in the physiological process of invertebrates. in this paper, we report a novel function of dap1 in the antivirus immunity of shrimp. a homologue gene of dap1 was cloned from marsupenaeus japonicus and named as mjdap-1. the full-le ... | 2017 | 28212834 |
| two white spot syndrome virus micrornas target the dorsal gene to promote virus infection in marsupenaeus japonicus shrimp. | in eukaryotes, micrornas (mirnas) serve as regulators of many biological processes, including virus infection. an mirna can generally target diverse genes during virus-host interactions. however, the regulation of gene expression by multiple mirnas has not yet been extensively explored during virus infection. this study found that the spaztle (spz)-toll-dorsal-antilipopolysaccharide factor (alf) signaling pathway plays a very important role in antiviral immunity against invasion of white spot sy ... | 2017 | 28179524 |
| interaction of the small gtpase cdc42 with arginine kinase restricts white spot syndrome virus in shrimp. | many types of small gtpases are widely expressed in eukaryotes and have different functions. as a crucial member of the rho gtpase family, cdc42 serves a number of functions, such as regulating cell growth, migration, and cell movement. several rna viruses employ cdc42-hijacking tactics in their target cell entry processes. however, the function of cdc42 in shrimp antiviral immunity is not clear. in this study, we identified a cdc42 protein in the kuruma shrimp (marsupenaeus japonicus) and named ... | 2017 | 28031362 |
| l-type lectin from the kuruma shrimp marsupenaeus japonicus promotes hemocyte phagocytosis. | l-type lectins (ltls) contain a luminal carbohydrate recognition domain, which exhibits homology to leguminous lectins. these type i membrane proteins are involved in the early secretory pathway of animals, and have functions in glycoprotein sorting, trafficking and targeting. recent studies suggest that ltls may be involved in immune responses in vertebrates, but no functional studies have been reported. this study reports an ltl, designated as mjltl1, from the kuruma shrimp marsupenaeus japoni ... | 2014 | 24508102 |
| akirin interacts with bap60 and 14-3-3 proteins to regulate the expression of antimicrobial peptides in the kuruma shrimp (marsupenaeus japonicus). | akirin is a recently discovered nuclear factor that plays important roles in innate immune responses. akirin is a positive regulator of the nf-κb factor of the drosophila immune deficiency (imd) pathway, which shares extensive similarities with the mammalian tumor necrosis factor receptor (tnfr) signaling pathway. however, some studies found that the nf-κb transcriptional targets were also strongly repressed in akirin2 knockout mice following tlr, il-1β and tnfα treatment. therefore, the functio ... | 2016 | 26493016 |
| gene silencing of vp9 gene impairs wssv infectivity on macrobrachium rosenbergii. | white spot syndrome virus (wssv) remains the most widespread and devastating infectious agent that hit the shrimp aquaculture industry worldwide. to date, there are no known effective strategies yet to combat wssv infection. hence, functional studies on genes critical for viral infection is essential in elucidating shrimp-virus interaction. here we report the function of a gene from wssv coding for a non-structural protein, vp9, utilizing rna interference. silencing of vp9 gene also effectively ... | 2016 | 26811904 |