Publications
| Title | Abstract | Year Filter | PMID(sorted ascending) Filter |
|---|
| molecular cloning and characterization of mj-mov-10, a putative rna helicase involved in rnai of kuruma shrimp. | identification and characterization of the rnai-related genes is the key to understanding rnai mechanism in shrimp. in this study, we have identified and characterized a novel putative rna helicase gene, mj-mov-10 from the kuruma shrimp, marsupenaeus japonicus and its implication in shrimp rnai was demonstrated. the full-length mj-mov-10 gene contained 3536bp, including 239 bp of 5'utr, 2895 bp of the open reading frame (orf) and 402bp of 3'utr, respectively. an orf of mj-mov-10 could be transla ... | 2015 | 25724627 |
| syntenin is involved in the bacteria clearance response of kuruma shrimp (marsupenaeus japonicus). | syntenin is a multifunctional cytosolic adaptor protein that contributes to cell migration, proliferation, attachment, and apoptosis, as well as immune response to virus, in vertebrates. however, the functions of syntenin in the antibacterial response of invertebrates remain unclear. in this study, we identified a syntenin-like gene (mjsyn) from the kuruma shrimp (marsupenaeus japonicus) and detected its function in the antibacterial immunity of shrimp. the full-length mjsyn was 1223 bp with a 9 ... | 2015 | 25731918 |
| molecular cloning, characterization, and expression analysis of two different types of lectins from the oriental river prawn, macrobrachium nipponense. | lectins, which are widely expressed in invertebrates, play important roles in many biological processes, including protein trafficking, cell signaling, pathogen recognition, as effector molecules, and so on (wang and wang, 2013). this study identified one novel m-type lectin and one l-type lectin, designated as mnmtl1 and mnltl1, from the oriental river prawn macrobrachium nipponense. the full-length cdna of mnmtl1 was 2064 bp with a 1761 bp orf encoding a putative protein of 586 deduced amino a ... | 2015 | 25929240 |
| a homologue gene of β-catenin participates in the development of shrimps and immune response to bacteria and viruses. | β-catenin is a multifunctional protein that is involved in many physiological processes, including development, cell proliferation, cell migration, and apoptosis. however, the function of β-catenin in crustacean is unknown. in this study, the first shrimp homologous gene of β-catenin in marsupenaeus japonicus (i.e., mjβ-catenin) was identified and characterized. the full-length of the complementary dna of mjβ-catenin is 3130 bp, including a 2463 bp open reading frame that encodes 821 amino acid. ... | 2015 | 26334791 |
| identification and expression analysis of a novel stylicin antimicrobial peptide from kuruma shrimp (marsupenaeus japonicus). | antimicrobial peptides (amps) are important components of the innate immune system and function as the first line of defense against invading pathogens. in current study we identified, cloned and characterized a novel stylicin amp from kuruma shrimp marsupenaeus japonicus (mj-sty). the full-length cdna of mj-sty was 428 bp with an open reading frame of 315 bp that encoded 104 amino acids. the theoretical molecular mass of mature mj-sty was 8.693 kda with an isoelectric point (pi) of 4.79. a prol ... | 2015 | 26439413 |
| differential expression of micrornas in shrimp marsupenaeus japonicus in response to vibrio alginolyticus infection. | till date numerous micrornas (mirnas) have been discovered from various organisms, including mammals, plants, insects, nematodes and viruses. they are known to have antiviral functions in crustaceans such as shrimp marsupenaeus japonicas. however, little is known about the role of mirnas against bacterial infection in this shrimp caused by vibrio alginolyticus. we performed small rna sequencing to characterize the differentially expressed micrornas in v. alginolyticus challenged shrimp, in compa ... | 2016 | 26483347 |
| the effects of a thermophile metabolite, tryptophol, upon protecting shrimp against white spot syndrome virus. | white spot syndrome virus (wssv) is a shrimp pathogen responsible for significant economic loss in commercial shrimp farms and until now, there has been no effective approach to control this disease. in this study, tryptophol (indole-3-ethanol) was identified as a metabolite involved in bacteriophage-thermophile interactions. the dietary addition of tryptophol reduced the mortality in shrimp marsupenaeus japonicus when orally challenged with wssv. our results revealed that 50 mg/kg tryptophol ha ... | 2015 | 26492993 |
| akirin interacts with bap60 and 14-3-3 proteins to regulate the expression of antimicrobial peptides in the kuruma shrimp (marsupenaeus japonicus). | akirin is a recently discovered nuclear factor that plays important roles in innate immune responses. akirin is a positive regulator of the nf-κb factor of the drosophila immune deficiency (imd) pathway, which shares extensive similarities with the mammalian tumor necrosis factor receptor (tnfr) signaling pathway. however, some studies found that the nf-κb transcriptional targets were also strongly repressed in akirin2 knockout mice following tlr, il-1β and tnfα treatment. therefore, the functio ... | 2016 | 26493016 |
| rna editing of microrna prevents rna-induced silencing complex recognition of target mrna. | micrornas (mirnas) integrate with argonaut (ago) to create the rna-induced silencing complex, and regulate gene expression by silencing target mrnas. rna editing of mirna may affect mirna processing, assembly of the ago complex and target mrna binding. however, the function of edited mirna, assembled within the ago complex, has not been extensively investigated. in this study, sequence analysis of the ago complex of marsupenaeus japonicus shrimp infected with white spot syndrome virus (wssv) rev ... | 2015 | 26674414 |
| identification and expression analysis of a new invertebrate lysozyme in kuruma shrimp (marsupenaeus japonicus). | lysozyme is an important component of the innate immunity system against invading pathogens. an invertebrate (i-type) lysozyme from the hepatopancreas of kuruma shrimp marsupenaeus japonicus (mj-ilys) was identified. the full-length cdna of mj-ilys was 580bp with a 429 bp open reading frame encoding a 142 amino acid polypeptide. the encoded polypeptide was predicted to have a 17 amino acid signal peptide, and a 125 amino acid mature protein with a theoretical mass of 14.099 kda and an isoelectri ... | 2016 | 26723264 |
| gene silencing of vp9 gene impairs wssv infectivity on macrobrachium rosenbergii. | white spot syndrome virus (wssv) remains the most widespread and devastating infectious agent that hit the shrimp aquaculture industry worldwide. to date, there are no known effective strategies yet to combat wssv infection. hence, functional studies on genes critical for viral infection is essential in elucidating shrimp-virus interaction. here we report the function of a gene from wssv coding for a non-structural protein, vp9, utilizing rna interference. silencing of vp9 gene also effectively ... | 2016 | 26811904 |
| dual oxidases participate in the regulation of intestinal microbiotic homeostasis in the kuruma shrimp marsupenaeus japonicus. | the metazoan gut lumen harbors numerous microbial communities. tolerance for high bacterial counts and maintenance of microbiota homeostasis remain insufficiently studied. in this study, we identified a novel dual oxidase (mjduox2) involved in reactive oxygen species (ros) production in the kuruma shrimp marsupenaeus japonicus. mjduox2 is a transmembrane protein with an n-signal peptide region (19 aa) and a peroxidase homology domain (phd, 554 aa) in the extracellular region; seven transmembrane ... | 2016 | 26845611 |
| carbon dioxide and methane fluxes from feeding and no-feeding mariculture ponds. | the co2 and ch4 fluxes at the water-air interface were measured in shrimp (marsupenaeus japonicus) monoculture ponds (s) with feed supply and shrimp-sea cucumber (apostichopus japonicus) polyculture ponds (ss) without feed supply. during farming seasons of the whole year, cumulated co2-c fluxes were -5.69 g m(-2) (s) and 11.23 g m(-2) (ss), respectively. the cumulated co2 emissions from s and ss did not differ significantly. the cumulated ch4-c emissions from s (0.57 g m(-2)) were significantly ... | 2016 | 26967535 |
| extracellular trap formation in kuruma shrimp (marsupenaeus japonicus) hemocytes is coupled with c-type lysozyme. | in invertebrates, hemocytes play an important role in immune responses. recently, a novel form of innate immune mechanism called extracellular traps (ets) was identified in shrimps, where dna and antimicrobial peptides form complex structure to entrap the invading microbes. in this study, we detected the formation of ets from hemocytes of kuruma shrimp in response to various stimulations, including phorbol myristate acetate (pma), lipopolysaccharide (lps), peptidoglycan (pgn) and escherichia col ... | 2016 | 27012393 |
| involvement of a lysm and putative peptidoglycan-binding domain-containing protein in the antibacterial immune response of kuruma shrimp marsupenaeus japonicus. | lysin motif (lysm) is a peptidoglycan and chitin-binding motif with multiple functions in bacteria, plants, and animals. in this study, a novel lysm and putative peptidoglycan-binding domain-containing protein was cloned from kuruma shrimp (marsupenaeus japonicus) and named as mjlpbp. the cdna of mjlpbp contained 1010 nucleotides with an open reading frame of 834 nucleotides encoding a protein of 277 amino acid residues. the deduced protein contained a lysin motif and a transmembrane region, wit ... | 2016 | 27142936 |
| a novel method of real-time reverse-transcription loop-mediated isothermal amplification developed for rapid and quantitative detection of a new genotype (yhv-8) of yellow head virus. | a new genotype of yellow head virus (yhv), designated as yhv-8, was found in farmed shrimp fenneropenaeus chinensis suffering suspectedly from ems/ahpns (early mortality disease/acute hepatopancreatic necrosis disease) in china in 2012. in this study, a one-step, real-time reverse-transcription loop-mediated isothermal amplification (rrt-lamp) assay was developed for better detection of both genotypes of yhv-1 and yhv-8. a set of six specific primers was successfully designed targeting a conserv ... | 2016 | 27221155 |
| characterization of a kunitz-type protease inhibitor (mjkupi) reveals the involvement of mjkupi positive hemocytes in the immune responses of kuruma shrimp marsupenaeus japonicus. | serine proteases and their inhibitors play vital roles in biological processes. serine protease inhibitors, including kunitz-type protease inhibitors play important roles not only in physiological process (i.e. blood clotting and fibrinolysis) but also in immune responses. in this study, we characterized a kunitz-type protease inhibitor, designated mjkupi, from kuruma shrimp marsupenaeus japonicus. an expression profile showed that mjkupi was mainly expressed in hemocytes. immunostaining reveale ... | 2016 | 27255219 |
| minichromosome maintenance protein 7 regulates phagocytosis in kuruma shrimp marsupenaeus japonicas against white spot syndrome virus. | minichromosome maintenance protein (mcm7) belongs to the mcm protein family and participates in the mcm complex by playing a role in the cell replication cycle and chromosome initiation of eukaryotes. previously, we found that several genes, including mcm7, were over-expressed in drosophila melanogaster after white spot syndrome virus (wssv) infection. in this study, we aimed to further research the mcm7 of kuruma shrimp, marsupenaeus japonicus (mjmcm7) and determine its role in the innate immun ... | 2016 | 27276115 |
| three members of ras gtpase superfamily are response to white spot syndrome virus challenge in marsupenaeus japonicus. | members of the ras-like gtpase superfamily are key regulators of diverse cellular and developmental events, including differentiation, cell division, vesicle transport, nuclear assembly, and cytoskeleton control. in this study, three ras family members (mjrap, mjras, and mjral) were cloned from marsupenaeus japonicus. the full lengths of mjrap, mjras, and mjral are 788, 1330, and 2074 bp, which encode the proteins of 186, 202, and 198 amino acids respectively. phylogenetic analysis showed that r ... | 2016 | 27349204 |
| molecular and immune response characterizations of a novel aif and cytochrome c in litopenaeus vannamei defending against wssv infection. | apoptosis inducing factor (aif) and cytochrome c (cyc) are two mitochondrial apoptogenic factors. in the present study, the cdna sequences of aif (lvaif) and cyc (lvcyc) were cloned from pacific white shrimp, litopenaeus vannamei. the lvaif was 1664 bp, including a 5'-terminal untranslated region (utr) of 154 bp, an open reading frame (orf) of 1323 bp encoding a polypeptide of 440 amino acids (aa) and a 3' utr of 187 bp. the lvcyc was 582 bp, including a 50 bp 5' utr, a 315 bp orf encoding for 1 ... | 2016 | 27368536 |
| suppressor of cytokine signaling 2 (socs2) negatively regulates the expression of antimicrobial peptides by affecting the stat transcriptional activity in shrimp marsupenaeus japonicus. | the suppressor of cytokine signaling (socs) family is a kind of negative regulators in the janus kinase/signal transducer and activator of transcription (jak/stat) pathway in mammals and drosophila. in kuruma shrimp, marsupenaeus japonicus, socs2 is identified and its expression can be stimulated by peptidoglycan and polycytidylic acid. however, if socs2 participates in regulating jak/stat pathway in shrimp still needs further study. in this study, socs2 with src homology 2 domain and socs box w ... | 2016 | 27492125 |
| virulence and antibiotic and heavy metal resistance of vibrio parahaemolyticus isolated from crustaceans and shellfish in shanghai, china. | vibrio parahaemolyticus can cause serious human seafoodborne gastroenteritis and even death. in this study, we isolated and characterized 208 v. parahaemolyticus strains from 10 species of commonly consumed crustaceans and shellfish available in fish markets in shanghai, people's republic of china, in 2014. most of these aquatic species had not been detected previously. the results revealed an extremely low occurrence of pathogenic v. parahaemolyticus carrying the toxin gene trh (1.9%). however, ... | 2016 | 27497124 |
| virulence of acute hepatopancreatic necrosis disease pirab-like relies on secreted proteins not on gene copy number. | to investigate the virulence of the vp_pirab-like genes in vibrio parahaemolyticus- acute hepatopancreatic necrosis disease (ahpnd)-causing strain and the factors that are associated with the virulence level. | 2016 | 27522063 |
| β-arrestin 1's interaction with tc45 attenuates stat signaling by dephosphorylating stat to inhibit antimicrobial peptide expression. | impaired phosphatase activity leads to the persistent activation of signal transducers and activators of transcription (stat). in mammals, stat family members are often phosphorylated or dephosphorylated by the same enzymes. to date, only one stat similar to mammalian stat5a/b has been found in crustaceans and there have been few studies in stat signal regulation in crustaceans. here, we report that β-arrestin1 interacts with tc45 (45-kda form of t cell protein tyrosine phosphatase) in the nucle ... | 2016 | 27782165 |
| scavenger receptor c mediates phagocytosis of white spot syndrome virus and restricts virus proliferation in shrimp. | scavenger receptors are an important class of pattern recognition receptors that play several important roles in host defense against pathogens. the class c scavenger receptors (srcs) have only been identified in a few invertebrates, and their role in the immune response against viruses is seldom studied. in this study, we firstly identified an src from kuruma shrimp, marsupenaeus japonicus, designated mjsrc, which was significantly upregulated after white spot syndrome virus (wssv) challenge at ... | 2016 | 28027319 |
| leucine-rich repeats containing protein functions in the antibacterial immune reaction in stomach of kuruma shrimp marsupenaeus japonicus. | leucine rich repeat (lrr) motif exists in many immune receptors of animals and plants. most lrr containing (lrrc) proteins are involved in protein-ligand and protein-protein interaction, but the exact functions of most lrrc proteins were not well-studied. in this study, an lrrc protein was identified from kuruma shrimp marsupenaeus japonicus, and named as mjlrrc1. mjlrrc1 was consistently expressed in different tissues of normal shrimp with higher expression in gills and stomach. at the transcri ... | 2017 | 28027987 |
| interaction of the small gtpase cdc42 with arginine kinase restricts white spot syndrome virus in shrimp. | many types of small gtpases are widely expressed in eukaryotes and have different functions. as a crucial member of the rho gtpase family, cdc42 serves a number of functions, such as regulating cell growth, migration, and cell movement. several rna viruses employ cdc42-hijacking tactics in their target cell entry processes. however, the function of cdc42 in shrimp antiviral immunity is not clear. in this study, we identified a cdc42 protein in the kuruma shrimp (marsupenaeus japonicus) and named ... | 2017 | 28031362 |
| purification and characterization of bioactive peptides ryamide and cchamide in the kuruma shrimp marsupenaeus japonicus. | to understand the regulation systems of appetite, bioactive peptides from the kuruma shrimp marsupenaeus japonicus (mj) were isolated and purified by reverse pharmacological assays using cho cells expressing the drosophila melanogaster g-protein-coupled receptors (gpcrs) cg5811 (a ryamide receptor) or cg14593 (a cchamide-2 receptor). four peptides having binding activity to gpcrs were obtained and named mj ryamide-1, mj ryamide-2, mj ryamide-3, and mj cchamide. genes encoding the prepropeptides ... | 2017 | 28062303 |
| two white spot syndrome virus micrornas target the dorsal gene to promote virus infection in marsupenaeus japonicus shrimp. | in eukaryotes, micrornas (mirnas) serve as regulators of many biological processes, including virus infection. an mirna can generally target diverse genes during virus-host interactions. however, the regulation of gene expression by multiple mirnas has not yet been extensively explored during virus infection. this study found that the spaztle (spz)-toll-dorsal-antilipopolysaccharide factor (alf) signaling pathway plays a very important role in antiviral immunity against invasion of white spot sy ... | 2017 | 28179524 |
| death associated protein 1 (dap 1) positively regulates virus replication and apoptosis of hemocytes in shrimp marsupenaeus japonicus. | death-associated protein 1 (dap1) is a small proline-rich cytoplasmic protein that functions both in the apoptosis and autophage process of mammalian and in the clinical cancer of human. however, little knowledge is known about the homologue gene of dap1 and its roles in the physiological process of invertebrates. in this paper, we report a novel function of dap1 in the antivirus immunity of shrimp. a homologue gene of dap1 was cloned from marsupenaeus japonicus and named as mjdap-1. the full-le ... | 2017 | 28212834 |
| effect of the dietary probiotic clostridium butyricum on growth, intestine antioxidant capacity and resistance to high temperature stress in kuruma shrimp marsupenaeus japonicus. | a 56-day feeding trial followed by an acute high temperature stress test were performed to evaluate the effect of dietary probiotic clostridium butyricum (cb) on growth performance and intestine antioxidant capacity of kuruma shrimp marsupenaeus japonicus. shrimp were randomly allocated in 9 tanks (30 shrimp per tank) and triplicate tanks were fed with diets containing different levels of c. butyricum (1×10(9) cfu/g): 0mgg(-1) feed (control), 100mgg(-1) feed (cb-100), 200mgg(-1) feed (cb-200) as ... | 2017 | 28477915 |
| the crustin-like peptide plays opposite role in shrimp immune response to vibrio alginolyticus and white spot syndrome virus (wssv) infection. | crustin is an antimicrobial peptide (amp) that plays a key role in innate immunity of crustaceans. in this study, we cloned the entire 660 bp crustin-like sequence with a 507 bp open reading frame encoding a 168 amino acid from marsupenaeus japonicus. the crustin-like gene was primarily expressed in gills and over-expressed in shrimp hemocytes after challenge with wssv or vibrio alginolyticus. after knockdown crustin-like gene using specific double-stranded rna (cru-dsrna), imd, rab7, l-lectin, ... | 2017 | 28546026 |
| scavenger receptor c promotes bacterial clearance in kuruma shrimp marsupenaeus japonicus by enhancing hemocyte phagocytosis and amp expression. | scavenger receptors (srs) comprise a large family of structurally diverse glycoproteins located on the cell membrane and function as pattern-recognition receptors (prrs) participating in innate immunity in different species. class c scavenger receptor (src) has been only identified in invertebrates and its biological functions still need to be researched. in this study, we characterized the anti-bacterial function of a src from kuruma shrimp marsupenaeus japonicus (mjsrc). the mrna level of mjsr ... | 2017 | 28602682 |
| shrimp mir-12 suppresses white spot syndrome virus infection by synchronously triggering antiviral phagocytosis and apoptosis pathways. | growing evidence has indicated that the innate immune system can be regulated by micrornas (mirnas). however, the mechanism underlying mirna-mediated simultaneous activation of multiple immune pathways remains unknown. to address this issue, the role of host mir-12 in shrimp (marsupenaeus japonicus) antiviral immune responses was characterized in the present study. the results indicated that mir-12 participated in virus infection, host phagocytosis, and apoptosis in defense against white spot sy ... | 2017 | 28824612 |
| activation of toll pathway is different between kuruma shrimp and drosophila. | the toll pathway is essential for inducing an immune response to defend against bacterial invasion in vertebrates and invertebrates. although toll receptors and the transcription factor dorsal were identified in different shrimp, relatively little is known about how the toll pathway is activated or the function of the pathway in shrimp antibacterial immunity. in this study, three tolls (toll1-3) and the dorsal were identified in marsupenaeus japonicus. the toll pathway can be activated by gram-p ... | 2017 | 28979261 |
| the shrimp hormone receptor acts as an anti-apoptosis and anti-inflammatory factor in innate immunity. | previously, we found that the expression of several genes, including hr, varied in drosophila melanogaster after white spot syndrome virus (wssv) infection. in this present study, we further investigated the role of hr in kuruma shrimp, marsupenaeus japonicus and determined its anti-apoptosis and anti-inflammation role in the innate immune system. we successfully identified a partial sequence (866 bp in length) of the m. japonicus hormone receptor ligand binding domain (mjhr_lbd/mjhr). the 5' en ... | 2018 | 29128494 |