Publications

TitleAbstractYear
Filter
PMID(sorted descending)
Filter
unveiling members of colletotrichum acutatum species complex causing colletotrichum leaf disease of hevea brasiliensis in sri lanka.colletotrichum is an important fungal genus with great diversity, which causes anthracnose of a variety of crop plants including rubber trees. colletotrichum acutatum and colletotrichum gloeosporioides have been identified as the major causative agents of colletotrichum leaf disease of rubber trees in sri lanka based on morphology, pathogenicity, and the analysis of internally transcribed spacer sequences of the nuclear ribosomal dna. this study has been conducted to investigate the members of t ...201728378161
construction of a system for the strawberry nursery production towards elimination of latent infection of anthracnose fungi by a combination of pcr and microtube hybridization.one of the major problems in strawberry production is difficulty in diagnosis of anthracnose caused by colletotrichum acutatum or glomerella cingulata in latent infection stage. we here developed a diagnostic tool for the latent infection consisting of initial culturing of fungi, dna extraction, synthesis of pcr-amplified probes and microtube hybridization (mth) using a macroarray. the initial culturing step is convenient to lure the fungi out of the plant tissues, and to extract pcr-inhibitor-f ...201728167891
effects of rhizobacteria paenibacillus polymyxa apec136 and >bacillus subtilis apec170 on biocontrol of postharvest pathogens of apple fruits.in this study, plant growth-promoting rhizobacteria (pgpr) were evaluated as potential biocontrol agents against postharvest pathogens of apple fruits. in vitro bioassays revealed that, out of 30 isolates screened, isolates apec136 and apec170 had the most significant inhibitory effects against the mycelial growth of several fungal pathogens. analysis of 16s ribosomal rna (rrna) sequences identified the two effective isolates as paenibacillus polymyxa and bacillus subtilis, respectively. the two ...201727921398
bacillus subtilis based-formulation for the control of postbloom fruit drop of citrus.postbloom fruit drop (pfd) caused by colletotrichum acutatum affects flowers and causes early fruit drop in all commercial varieties of citrus. biological control with the isolate acb-69 of bacillus subtilis has been considered as a potential method for controlling this disease. this study aimed to develop and optimize a b. subtilis based-formulation with a potential for large-scale applications and evaluate its effect on c. acutatum in vitro and in vivo. bacillus subtilis based-formulations wer ...201627804104
antimicrobial activity and chemical composition of three essential oils extracted from mediterranean aromatic plants.there is a growing interest in essential oils (eos) as possible alternatives for traditional chemical pesticides. this study was carried out to characterize the chemical composition of the three eos extracted from verbena officinalis, majorana hortensis, and salvia officinalis using gas chromatography (gc) and gc-mass spectrometry (gc-ms) and to evaluate in vitro their efficacy against some phyto or human pathogens. the antifungal activity was investigated against colletotrichum acutatum and bot ...201627792456
role of the colletotrichum acutatum sesquiterpene synthase catps in the biosynthesis of sesquiterpenoids.colletotrichum acutatum is a major fungal pathogen of fruit crops, which causes severe yield losses in strawberry production. a potential key factor in plant-pathogen interactions is fungal sesquiterpenoids which have mycotoxic and phytotoxic activities. the first committed step in sesquiterpenoid biosynthesis is performed by sesquiterpene synthases (tps). only a few tpss have been functionally characterized from filamentous fungi and none from the genus colletotrichum. despite being an importan ...201627553953
gene family expansions and contractions are associated with host range in plant pathogens of the genus colletotrichum.many species belonging to the genus colletotrichum cause anthracnose disease on a wide range of plant species. in addition to their economic impact, the genus colletotrichum is a useful model for the study of the evolution of host specificity, speciation and reproductive behaviors. genome projects of colletotrichum species have already opened a new era for studying the evolution of pathogenesis in fungi.201627496087
partial activation of sa- and ja-defensive pathways in strawberry upon colletotrichum acutatum interaction.understanding the nature of pathogen host interaction may help improve strawberry (fragaria × ananassa) cultivars. plant resistance to pathogenic agents usually operates through a complex network of defense mechanisms mediated by a diverse array of signaling molecules. in strawberry, resistance to a variety of pathogens has been reported to be mostly polygenic and quantitatively inherited, making it difficult to associate molecular markers with disease resistance genes. colletotrichum acutatum s ...201627471515
inactivation of plant-pathogenic fungus colletotrichum acutatum with natural plant-produced photosensitizers under solar radiation.the increasing tolerance to currently used fungicides and the need for environmentally friendly antimicrobial approaches have stimulated the development of novel strategies to control plant-pathogenic fungi such as antimicrobial phototreatment (apt). we investigated the in vitro apt of the plant-pathogenic fungus colletotrichum acutatum with furocoumarins and coumarins and solar radiation. the compounds used were: furocoumarins 8-methoxypsoralen (8-mop) and 5,8-dimethoxypsoralen (isopimpinellin) ...201627434699
changes in the aggressiveness and fecundity of hot pepper anthracnose pathogen (colletotricum acutatum) under elevated co2 and temperature over 100 infection cycles.we observed the changes in aggressiveness and fecundity of the anthracnose pathogen colletotrichum acutatum on hot pepper, under the ambient and the twice-ambient treatments. artificial infection was repeated over 100 cycles for ambient (25°c/400 ppm co2) and twice-ambient (30°c/700 ppm co2) growth chamber conditions, over 3 years. during repeated infection cycles (ics) on green-pepper fruits, the aggressiveness (incidence [% of diseased fruits among 20 inoculated fruits] and severity [lesion le ...201627298601
evaluation of leaf wetness duration models for operational use in strawberry disease-warning systems in four us states.leaf wetness duration (lwd) plays a key role in disease development and is often used as an input in disease-warning systems. lwd is often estimated using mathematical models, since measurement by sensors is rarely available and/or reliable. a strawberry disease-warning system called "strawberry advisory system" (sas) is used by growers in florida, usa, in deciding when to spray their strawberry fields to control anthracnose and botrytis fruit rot. currently, sas is implemented at six locations, ...201627180263
whole genome sequence and genome annotation of colletotrichum acutatum, causal agent of anthracnose in pepper plants in south korea.colletotrichum acutatum is a destructive fungal pathogen which causes anthracnose in a wide range of crops. here we report the whole genome sequence and annotation of c. acutatum strain kc05, isolated from an infected pepper in kangwon, south korea. genomic dna from the kc05 strain was used for the whole genome sequencing using a pacbio sequencer and the miseq system. the kc05 genome was determined to be 52,190,760 bp in size with a g + c content of 51.73% in 27 scaffolds and to contain 13,559 g ...201627114908
species of the colletotrichum acutatum complex associated with anthracnose diseases of fruit in brazil.although colletotrichum acutatum was recently investigated and shown to be a species complex comprising about 30 species, the name is still used in its broad sense for anthracnose pathogens of fruits in brazil. in this study, a multilocus molecular analysis was carried out based on a dataset of its, his3, gapdh, chs-1, tub2 and act sequences of colletotrichum strains belonging to the c. acutatum species complex from fruits collected in different regions in brazil combined with sequences of ex-ty ...201627020156
antifungal new oxepine-containing alkaloids and xanthones from the deep-sea-derived fungus aspergillus versicolor scsio 05879.phytopathogenic fungi remain a continuous and huge threat in the agricultural fields. the agrochemical industry has made great development of the use of microbial natural products, which has been regarded as an effective strategy against phytopathogenic fungi. antifungal bioassay-directed fractionation was used to isolate two new oxepine-containing alkaloids (1 and 2), two new 4-aryl-quinolin-2-one alkaloids (3 and 4), and four new prenylated xanthones (5-8) from the deep-sea-derived fungus aspe ...201626998701
polyphenols variation in fruits of the susceptible strawberry cultivar alba during ripening and upon fungal pathogen interaction and possible involvement in unripe fruit tolerance.strawberry (fragaria × ananassa) fruit contains high concentrations of health-promoting phenolic compounds, playing important roles for the fruit ontogenic tolerance to fungi. in the highly susceptible cultivar alba, the two major strawberry fungal pathogens, colletotrichum acutatum and botrytis cinerea, displayed disease symptoms only at red ripe stages because immature fruits are tolerant to diseases. we analyzed and compared the variation of 47 polyphenols in the surface of unripe and ripe al ...201626895094
characterization of a colletotrichum population causing anthracnose disease on olive in northern tunisia.to phenotypically, physiologically and molecularly characterize the causal agent of olive anthracnose in the northern tunisia and to study its genetic variability and pathogenicity.201626876102
an in vitro attempt for controlling severe phytopathogens and human pathogens using essential oils from mediterranean plants of genus schinus.growing concerns about food safety and environmental protection enhanced the need for new and safe plant disease control strategies. the chemical composition of the three essential oils (eos) extracted from leaves and fruits of schinus terebinthifolius and leaves of schinus molle, growing in tunisia, was studied by gc and gc-ms. in all, 12 compounds were identified. the oils were mainly composed of terpene compounds. α-pinene, α-phellandrene, and d-limonene were the major constituents. the aim o ...201626836214
antagonistic activity of fungi of olea europaea l. against colletotrichum acutatum.fungi naturally present in olive trees were identified and tested for their antagonistic potential against colletotrichum acutatum. a total of 14 isolates were identified, 12 belonged to genera alternaria, epicoccum, fusarium, aspergillus, anthrinium, chaetomium, diaporthe, nigrospora, one to family xylariaceae and one was unclassified. all fungal isolates showed some inhibitory action over the growth of c. acutatum during dual culture growth, however, when agar-diffusible tests were performed o ...201626805623
efficacy of sdhi fungicides, including benzovindiflupyr, against colletotrichum species.colletotrichum species cause anthracnose diseases on many plants and crops. a new generation of succinate dehydrogenase inhibitors (sdhis) was developed recently. the inhibitory activity of the five sdhi fungicides against colletotrichum species was determined in this study.201626732510
growth simulation and discrimination of botrytis cinerea, rhizopus stolonifer and colletotrichum acutatum using hyperspectral reflectance imaging.this research aimed to develop a rapid and nondestructive method to model the growth and discrimination of spoilage fungi, like botrytis cinerea, rhizopus stolonifer and colletotrichum acutatum, based on hyperspectral imaging system (his). a hyperspectral imaging system was used to measure the spectral response of fungi inoculated on potato dextrose agar plates and stored at 28°c and 85% rh. the fungi were analyzed every 12 h over two days during growth, and optimal simulation models were built ...201526642054
the complete mitochondrial genome sequence of the ascomycete plant pathogen colletotrichum acutatum.collectotrichum acutatum is a fungal plant pathogen that causes pre- and post-harvest anthracnose on a wide range of plants worldwide. the complete mitochondrial genome of c. acutatum has been determined for the first time. this study revealed that the mitogenome of c. acutatum is a closed circular molecule of 30 892 bp in length, with a g + c content of 34.7%, which include 15 protein-coding genes, 22 trna genes, and two rrna genes. all the protein-coding genes, accounting for 46.6% of the c. a ...201626539901
postharvest biological control of colletotrichum acutatum on apple by bacillus subtilis hm1 and the structural identification of antagonists.bacillus subtilis hm1 was isolated from the rhizosphere region of halophytes for its antifungal activity against colletotrichum acutatum, the causative agent of anthracnose. treatment of postharvest apples with the cell culture or with a cell-free culture supernatant reduced disease severity 80.7% and 69.4%, respectively. both treatments also exhibited antifungal activity against various phytopathogenic fungi in vitro. the antifungal substances were purified and analyzed by acid precipitation, g ...201526428548
ultrastructural changes in the epidermis of petals of the sweet orange infected by colletotrichum acutatum.postbloom fruit drop (pfd) is an important disease caused by the fungus colletotrichum acutatum. pfd is characterised by the formation of necrotic lesions on the petals and stigmas of flowers as well as premature abscission of the fruit in citrus spp. we compare the ultrastructure of the epidermis of uninoculated citrus sinensis petals with that of petals inoculated with the fungus to understand the changes that occur upon c. acutatum infection. healthy petals have a cuticle with parallel striat ...201626334287
a revision of the history of the colletotrichum acutatum species complex in the nordic countries based on herbarium specimens.herbaria collections containing plants with disease symptoms are highly valuable, and they are often the only way to investigate outbreaks and epidemics from the past as the number of viable isolates in culture collections is often limited. species belonging to the colletotrichum acutatum complex infect a range of important crops. as members of the c. acutatum complex are easily confused with other colletotrichum species, molecular methods are central for the correct identification. we performed ...201526296707
unfoldome variation upon plant-pathogen interactions: strawberry infection by colletotrichum acutatum.intrinsically disordered proteins (idps) are proteins that lack secondary and/or tertiary structure under physiological conditions. these proteins are very abundant in eukaryotic proteomes and play crucial roles in all molecular mechanisms underlying the response to environmental challenges. in plants, different idps involved in stress response have been identified and characterized. nevertheless, a comprehensive evaluation of protein disorder in plant proteomes under abiotic or biotic stresses ...201526245354
chemical composition and biological activity of essential oils from wild growing aromatic plant species of skimmia laureola and juniperus macropoda from western himalaya.the himalayan region is very rich in a great variety of medicinal plants. in this investigation the essential oils of two selected species are described for their antimicrobial and larvicidal as well as biting deterrent activities. additionally, the odors are characterized. analyzed by simultaneous gc-ms and gc-fid, the essential oils' chemical compositions are given. the main components of skimmia laureola oil were linalool and linalyl acetate whereas sabinene was found as the main compound for ...201526197554
expression of the β-1,3-glucanase gene bgn13.1 from trichoderma harzianum in strawberry increases tolerance to crown rot diseases but interferes with plant growth.the expression of antifungal genes from trichoderma harzianum, mainly chitinases, has been used to confer plant resistance to fungal diseases. however, the biotechnological potential of glucanase genes from trichoderma has been scarcely assessed. in this research, transgenic strawberry plants expressing the β-1,3-glucanase gene bgn13.1 from t. harzianum, under the control of the camv35s promoter, have been generated. after acclimatization, five out of 12 independent lines analysed showed a stunt ...201526178245
molecular diversity of anthracnose pathogen populations associated with uk strawberry production suggests multiple introductions of three different colletotrichum species.fragaria × ananassa (common name: strawberry) is a globally cultivated hybrid species belonging to rosaceae family. colletotrichum acutatum sensu lato (s.l.) is considered to be the second most economically important pathogen worldwide affecting strawberries. a collection of 148 colletotrichum spp. isolates including 67 c. acutatum s.l. isolates associated with the phytosanitary history of uk strawberry production were used to characterize multi-locus genetic variation of this pathogen in the uk ...201526086351
antagonistic activities of bacillus spp. strains isolated from tidal flat sediment towards anthracnose pathogens colletotrichum acutatum and c. gloeosporioides in south korea.anthracnose is a fungal disease caused by colletotrichum species that is detrimental to numerous plant species. anthracnose control with fungicides has both human health and environmental safety implications. despite increasing public concerns, fungicide use will continue in the absence of viable alternatives. there have been relatively less efforts to search antagonistic bacteria from mudflats harboring microbial diversity. a total of 420 bacterial strains were isolated from mudflats near the w ...201526060435
saccharomyces cerevisiae: a novel and efficient biological control agent for colletotrichum acutatum during pre-harvest.in this study, we evaluated the efficiency of six isolates of saccharomyces cerevisiae in controlling colletotrichum acutatum, the causal agent of postbloom fruit drop that occur in pre-harvest citrus. we analyzed the mechanisms of action involved in biological control such as: production of antifungal compounds, nutrient competition, detection of killer activity, and production of hydrolytic enzymes of the isolates of s. cerevisiae on c. acutatum and their efficiency in controlling postbloom fr ...201525960430
ieodoglucomide c and ieodoglycolipid, new glycolipids from a marine-derived bacterium bacillus licheniformis 09idym23.chemical examination of the ethyl acetate extract from the fermentation broth of the marine-derived bacterium bacillus licheniformis resulted in the isolation of two new glycolipids, ieodoglucomide c (1) and ieodoglycolipid (2). the structural characterization of 1 and 2 was achieved by extensive spectroscopic evidence, including 2d nmr experiments. a combination of chemical derivatization techniques followed by nmr studies, lc-ms data analysis and a literature review was deployed for the establ ...201525893812
loop-mediated isothermal amplification (lamp) based detection of colletotrichum falcatum causing red rot in sugarcane.red rot, caused by colletotrichum falcatum, is a destructive disease prevalent in most sugarcane-producing countries. disease-free sugarcane planting materials (setts) are essential as the pathogen spreads primarily through infected setts. the present study was undertaken to develop a loop-mediated isothermal amplification (lamp) assay for the detection of c. falcatum. c. falcatum genomic dna was isolated from pure mycelium culture and infected tissues. four sets of primers corresponding to a un ...201525861736
control of human and plant fungal pathogens using pentaene macrolide 32, 33-didehydroroflamycoin.the aim of this study was to address the toxicity of recently described polyene macrolide 32, 33-didehydroroflamycoin (ddhr) on a wide range of fungal pathogens and its potential to control plant fungal diseases.201525810243
gas chromatography-mass spectrometry method optimized using response surface modeling for the quantitation of fungal off-flavors in grapes and wine.an optimized method for the quantitation of volatile compounds responsible for off-aromas, such as earthy odors, found in wine and grapes was developed. the method involved a fast and simple headspace solid-phase microextraction-gas chromatography-mass spectrometry (hs-spme-gc-ms) for simultaneous determination of 2-isopropyl-3-methoxypyrazine, 2-isobutyl-3-methoxypyrazine, 3-octanone, fenchone, 1-octen-3-one, trans-2-octen-1-ol, fenchol, 1-octen-3-ol, 2-methylisoborneol, 2,4,6-trichloroanisole, ...201525703150
pcr-based specific techniques used for detecting the most important pathogens on strawberry: a systematic review.strawberry diseases are a major limiting factor that severely impact plant agronomic performance. regarding limitations of traditional techniques for detection of pathogens, researchers have developed specific dna-based tests as sensitive and specific techniques. the aim of this review is to provide an overview of polymerase chain reaction (pcr)-based methods used for detection or quantification of the most widespread strawberry pathogens, such as fusarium oxysporum f.sp. fragariae, phytophthora ...201525588564
growth under visible light increases conidia and mucilage production and tolerance to uv-b radiation in the plant pathogenic fungus colletotrichum acutatum.light conditions can influence fungal development. some spectral wavebands can induce conidial production, whereas others can kill the conidia, reducing the population size and limiting dispersal. the plant pathogenic fungus colletotrichum acutatum causes anthracnose in several crops. during the asexual stage on the host plant, colletototrichum produces acervuli with abundant mucilage-embedded conidia. these conidia are responsible for fungal dispersal and host infection. this study examined the ...201525535947
structural and biochemical characteristics of citrus flowers associated with defence against a fungal pathogen.the constitutive characters of plants can be structural or biochemical and play an important role in their defence against pathogens. citrus postbloom fruit drop (pfd) caused by colletotrichum spp. is one of the most important fungal diseases of citrus. the pathogen infects the flowers, leading to premature fruit drop and reducing citrus production. however, flower buds smaller than 8 mm long are usually not infected by colletotrichum spp. thus, this study investigated whether there are constitu ...201425535209
isolation and characterization of the colletotrichum acutatum abc transporter caabc1.fungi tolerate exposure to various abiotic stresses, including cytotoxic compounds and fungicides, via their atp-driven efflux pumps belonging to atp-binding cassette (abc) transporters. to clarify the molecular basis of interaction between the fungus and various abiotic stresses including fungicides, we constructed a cdna library from germinated conidia of colletotrichum acutatum, a major anthracnose pathogen of pepper (capsicum annum l.). over 1,000 cdna clones were sequenced, of which single ...201425506302
bacillus cereus ar156-induced resistance to colletotrichum acutatum is associated with priming of defense responses in loquat fruit.the effectiveness of a biocontrol agent bacillus cereus ar156 for control of anthracnose rot caused by colletotrichum acutatum in harvested loquat fruit and the possible mechanisms of its action have been investigated. treatment of fruit with b. cereus ar156 resulted in lower disease incidence and smaller lesion diameters compared with that of untreated fruit. the treatment enhanced activities of defense-related enzymes including chitinase, β-1, 3-glucanase, phenylalanine ammonia-lyase, peroxida ...201425386680
selection and a 3-year field trial of sorangium cellulosum kyc 3262 against anthracnose in hot pepper.kyc 3262 was selected as a biocontrol agent against anthracnose on hot pepper from 813 extracts of myxobacterial isolates. dual culture with colletotrichum acutatum and 813 myxobacterial extracts was conducted, and 19 extracts were selected that inhibited germination and mycelial growth of c. acutatum. all selections were sorangium cellulosum, which are cellulolytic myxobacteria from soil. with the infection bioassay on detached fruits in airtight containers, kyc 3262, kyc 3512, kyc 3279, and ky ...201425289014
biocontrol of phytophthora blight and anthracnose in pepper by sequentially selected antagonistic rhizobacteria against phytophthora capsici.we previously developed a sequential screening procedure to select antagonistic bacterial strains against phytophthora capsici in pepper plants. in this study, we used a modified screening procedure to select effective biocontrol strains against p. capsici; we evaluated the effect of selected strains on phytophthora blight and anthracnose occurrence and fruit yield in pepper plants under field and plastic house conditions from 2007 to 2009. we selected four potential biocontrol strains (pseudomo ...201325288942
antifungal compounds from turmeric and nutmeg with activity against plant pathogens.the antifungal activity of twenty-two common spices was evaluated against plant pathogens using direct-bioautography coupled colletotrichum bioassays. turmeric, nutmeg, ginger, clove, oregano, cinnamon, anise, fennel, basil, black cumin, and black pepper showed antifungal activity against the plant pathogens colletotrichum acutatum, colletotrichum fragariae, and colletotrichum gloeosporioides. among the active extracts, turmeric and nutmeg were the most active and were chosen for further investi ...201425173461
molecular and phytochemical investigation of angelica dahurica and angelica pubescentis essential oils and their biological activity against aedes aegypti, stephanitis pyrioides, and colletotrichum species.in this study, angelica dahurica and angelica pubescentis root essential oils were investigated as pest management perspectives, and root samples were also analyzed genetically using the nuclear ribosomal internal transcribed spacer (its) region as a dna barcode marker. a. pubescentis root essential oil demonstrated weak antifungal activity against colletotrichum acutatum, colletotrichum fragariae, and colletotrichum gloeosporioides, whereas a. dahurica root essential oil did not show antifungal ...201425133520
chemical composition and biological effects of artemisia maritima and artemisia nilagirica essential oils from wild plants of western himalaya.artemisia species possess pharmacological properties that are used for medical purposes worldwide. in this paper, the essential oils from the aerial parts of artemisia nilagirica and artemisia maritima from the western indian himalaya region are described. the main compounds analyzed by simultaneous gc/ms and gc/fid were camphor and 1,8-cineole from a. maritima, and camphor and artemisia ketone from a. nilagirica. additionally, the oils were evaluated for their antibacterial, antifungal, mosquit ...201425127023
molecular, phenotypic and pathogenic variability in colletotrichum isolates of subtropical region in north-eastern india, causing fruit rot of chillies.to study the genetic variability and population structure of colletotrichum species found to be associated with anthracnose of chilli in the north-eastern region of india.201425066262
isolation and characterization of genetic variability in bacteria with β-hemolytic and antifungal activity isolated from the rhizosphere of medicago truncatula plants.in the present study, we analyzed the frequency of hemolytic and antifungal activities in bacterial isolates from the rhizosphere of medicago truncatula plants. of the 2000 bacterial colonies, 96 showed β-hemolytic activities (frequency, 4.8 x 10(-2)). hemolytic isolates were analyzed for their genetic diversity by using random amplification of polymorphic dna, yielding 88 haplotypes. the similarity coefficient of nei and li showed a polymorphic diversity ranging from 0.3 to 1. additionally, 8 o ...201425062484
detection and characterization of a novel gammapartitivirus in the phytopathogenic fungus colletotrichum acutatum strain hnzj001.spherical virus-like particles about 40nm in diameter were observed under transmission electron microscope (tem) and two dsrna bands (dsrna-1 and dsrna-2) were detected on agarose gel after extraction from the mycelial preparation of a colletotrichum acutatum strain hnzj001 that isolated from an anthracnose lesion on immature pepper fruit. the complete nucleotide sequences of the dsrnas were determined. dsrna-1 (1762 nt) and dsrna-2 (1381 nt) each contained a single open reading frame and potent ...201425008759
antimicrobial activity of controlled-release chlorine dioxide gas on fresh blueberries.the effect of chlorine dioxide (clo2) gas on the safety and quality of blueberries was studied. in vitro studies revealed that both clo2 gas fumigation and clo2 direct contact in water killed food pathogen bacterium escherichia coli and fruit decay pathogen fungus colletotrichum acutatum. in vivo studies were conducted using noninoculated berries and berries inoculated with postharvest decay and foodborne pathogens. berries were inoculated with either e. coli (5.2 log cfu/g) or c. acutatum (3.9 ...201424988018
marine-derived penicillium in korea: diversity, enzyme activity, and antifungal properties.the diversity of marine-derived penicillium from korea was investigated using morphological and multigene phylogenetic approaches, analyzing sequences of the internal transcribed spacer region, β-tubulin gene, and rna polymerase subunit ii gene. in addition, the biological activity of all isolated strains was evaluated. we tested for the extracellular enzyme activity of alginase, endoglucanase, and β-glucosidase, and antifungal activity against two plant pathogens (colletotrichum acutatum and fu ...201424908060
non-cytotoxic antifungal agents: isolation and structures of gageopeptides a-d from a bacillus strain 109ggc020.antifungal resistance and toxicity problems of conventional fungicides highlighted the requirement of search for new safe antifungal agents. to comply with the requirement, we discovered four new non-cytotoxic lipopeptides, gageopeptides a-d, 1-4, from a marine-derived bacterium bacillus subtilis. the structures and stereochemistry of gageopeptides were determined by nmr data analysis and chemical means. gageopeptides exhibited significant antifungal activities against pathogenic fungi rhizocton ...201424857413
draft genome sequence of colletotrichum acutatum sensu lato (colletotrichum fioriniae).in addition to its economic impact, colletotrichum acutatum sensu lato is an interesting model for molecular investigations due to the diversity of host-determined specialization and reproductive lifestyles within the species complex. the pathogen colletotrichum fioriniae forms part of this species complex and causes anthracnose in a wide range of crops and wild plants worldwide. some members of this species have also been reported to be entomopathogenic. here, we report the draft genome sequenc ...201424723700
the mannose-binding lectin gene fambl1 is involved in the resistance of unripe strawberry fruits to colletotrichum acutatum.the fungal pathogen colletotrichum acutatum is the causal agent of strawberry (fragaria × ananassa) anthracnose. although the fungus can infect strawberry fruits at both unripe and ripe stages, the symptoms appear only on red ripe fruits. on white unripe fruits, the pathogen becomes quiescent as melanized appressoria after 24 h of interaction. previous transcriptome analysis has indicated that a mannose-binding lectin (mbl) gene is the most up-regulated gene in 24-h-infected white strawberries, ...201424690196
characterization of volatile constituents from origanum onites and their antifungal and antibacterial activity.essential oils obtained by hydrodistillation (hd) and microwave-assisted hd (mwhd) of origanum onites aerial parts were analyzed by gc and gcims. thirty-one constituents representing 98.6% of the water-distilled oil and 52 constituents representing 99.6% of the microwave-distilled oil were identified. carvacrol (76.8% hd and 79.2% mwhd) and thymol (4.7% hd and 4.4% mwhd) were characterized as major constituents in both essential oils. separation of carvacrol and thymol was achieved by overpressu ...201424645494
gene silencing and gene expression in phytopathogenic fungi using a plant virus vector.rna interference (rnai) is a powerful approach for elucidating gene functions in a variety of organisms, including phytopathogenic fungi. in such fungi, rnai has been induced by expressing hairpin rnas delivered through plasmids, sequences integrated in fungal or plant genomes, or by rnai generated in planta by a plant virus infection. all these approaches have some drawbacks ranging from instability of hairpin constructs in fungal cells to difficulties in preparing and handling transgenic plant ...201424594602
furocoumarins and coumarins photoinactivate colletotrichum acutatum and aspergillus nidulans fungi under solar radiation.the increasing tolerance to currently-used fungicides is a major problem both in clinical and agricultural areas leading to an urgent need for the development of novel antifungal strategies. this study investigated the in vitro antimicrobial photo treatment (apt) of conidia of the plant-pathogenic fungus colletotrichum acutatum and the ascomycete aspergillus nidulans with the furocoumarins 8-methoxypsoralen (8-mop) and isopimpinellin, and a mixture of two coumarins (7-methoxy coumarin and citrop ...201424509069
recent introduction and recombination in colletotrichum acutatum populations associated with citrus postbloom fruit drop epidemics in são paulo, brazil.citrus crops in são paulo state, brazil, have been severely affected by postbloom fruit drop disease (pfd), which is caused by colletotrichum acutatum. this disease leads to the drop of up to 100% of young fruits. previous studies have assumed that this pathogen exhibits a clonal reproductive mode, although no population genetic studies have been conducted so far. thus, the genetic structure of six c. acutatum populations from sweet orange orchards showing pfd symptoms was determined using nine ...201424423403
functional properties and antifungal activity of films based on gliadins containing cinnamaldehyde and natamycin.gliadin films cross-linked with cinnamaldehyde (1.5, 3, and 5%) and incorporated with natamycin (0.5%) were prepared by casting, and their antifungal activity, water resistance, and barrier properties were characterized. incorporation of natamycin gave rise to films with greater water uptake, weight loss and diameter gain, and higher water vapor and oxygen permeabilities. these results may be associated to a looser packing of the protein chains as a consequence of the presence of natamycin. the ...201424412960
in vitro photodynamic inactivation of plant-pathogenic fungi colletotrichum acutatum and colletotrichum gloeosporioides with novel phenothiazinium photosensitizers.the increasing tolerance to currently used fungicides in both clinical and agricultural areas is of great concern. the nonconventional light-based approach of antimicrobial photodynamic treatment (apdt) is a promising alternative to conventional fungicides. we evaluated the effects of apdt with four phenothiazinium derivatives (methylene blue [mb], new methylene blue n [nmbn], toluidine blue o [tbo], and the novel pentacyclic phenothiazinium photosensitizer [ps] s137) on conidia of three fungal ...201424362436
methyl anthranilate and γ-decalactone inhibit strawberry pathogen growth and achene germination.plant volatile compounds have been shown to affect microbial growth and seed germination. here two fruity volatiles found in strawberry ( fragaria × ananassa ), γ-decalactone ("peachlike" aroma) and methyl anthranilate ("grapelike" aroma), were tested for effects on relevant pathogens and seedling emergence. significant growth reduction was observed for botrytis cinerea , colletotrichum gloeosporioides , colletotrichum acutatum , phomopsis obscurans , and gnomonia fragariae at 1 mm γ-decalactone ...201324328200
differential resistances to anthracnose in capsicum baccatum as responding to two colletotrichum pathotypes and inoculation methods.chili anthracnose, caused by colletotrichum spp., is one of the major diseases to chili production in the tropics and subtropics worldwide. breeding for durable anthracnose resistance requires a good understanding of the resistance mechanisms to different pathotypes and inoculation methods. this study aimed to investigate the inheritances of differential resistances as responding to two different colletotrichum pathotypes, pca2 and pca3 and as by two different inoculation methods, microinjection ...201324273429
control of postharvest fungal pathogens by antifungal compounds from penicillium expansum.the fungicidal effects of secondary metabolites produced by a strain of penicillium expansum (r82) in culture filtrate and in a double petri dish assay were tested against one isolate each of botrytis cinerea, colletotrichum acutatum, and monilinia laxa and six isolates of p. expansum, revealing inhibitory activity against every pathogen tested. the characterization of volatile organic compounds released by the r82 strain was performed by solid-phase microextraction-gas chromatographic technique ...201324215691
effect of meja treatment on polyamine, energy status and anthracnose rot of loquat fruit.the effect of methyl jasmonate (meja) on changes in polyamines content and energy status and their relation to disease resistance was investigated. freshly harvested loquat fruit were treated with 10 μmol l(-1) meja and wound inoculated with colletotrichum acutatum spore suspension (1.0 × 10(5) spores ml(-1)) after 24h, and then stored at 20 °c for 6 days. meja treatment significantly reduced decay incidence. meja treated fruit manifested higher contents of polyamines (putrescine, spermidine and ...201424128452
occurrence of anthracnose on fruits of asian pear tree caused by colletotrichum acutatum.anthracnose symptoms often occurred on fruits of asian pear trees grown in anseong, naju, seonghwan and pyeongtaek in korea during the harvesting period from 2000 to 2005. a total of 28 isolates of colletotrichum sp. were obtained from the anthracnose symptoms. all the isolates were identified as colletotrichum acutatum based on their morphological and cultural characteristics. four isolates of the fungus were tested for pathogenicity to fruits of asian pear tree by artificial inoculation. all t ...200724015105
occurrence of anthracnose on highbush blueberry caused by colletotrichum species in korea.a total of 82 isolates of colletotrichum species were obtained from anthracnose symptoms of highbush blueberry trees grown in the gochang area of korea during a disease survey in 2008. out of the isolates, 75 were identified as colletotrichum gloeosporioides and the others as c. acutatum based on their morphological and cultural characteristics. twenty six of c. gloeosporioides isolates produced their teleomorph glomerella cingulata in pda culture. three isolates of each c. gloeosporioides and c ...200923983555
antifungal, insecticidal, and plant growth promoting potential of streptomyces hydrogenans dh16.in the present study, an actinobacterium strain, possessing antagonistic activity against different fungal phytopathogens viz. colletotrichum acutatum, cladosporium herbarum, alternaria brassicicola, exserohilum sp., alternaria mali, colletotrichum gleospoiroides, alternaria alternata, cercospora sp., fusarium oxysporum f.sp. dianthi and fusarium moniliformae, was isolated from soil and identified as streptomyces hydrogenans dh16. application of culture supernatant (5%)/cells (10(7)  cfu ml(-1) ...201423765423
light quality influences the virulence and physiological responses of colletotrichum acutatum causing anthracnose in pepper plants.to explore the effects of light quality on the physiology and pathogenicity of colletotrichum acutatum, we analysed the morphological traits, melanin production and virulence of the pathogen under different light wavelengths.201323663215
antifungal activity against plant pathogens of metabolites from the endophytic fungus cladosporium cladosporioides.bioassay-guided fractionation of cladosporium cladosporioides (fresen.) de vries extracts led to the isolation of four compounds, including cladosporin, 1; isocladosporin, 2; 5'-hydroxyasperentin, 3; and cladosporin-8-methyl ether, 4. an additional compound, 5',6-diacetylcladosporin, 5, was synthesized by acetylation of compound 3. compounds 1-5 were evaluated for antifungal activity against plant pathogens. phomopsis viticola was the most sensitive fungus to the tested compounds. at 30 μm, comp ...201323651409
chemical composition, antifungal and insecticidal activities of hedychium essential oils.the antimicrobial properties of essential oils have been documented, and their use as "biocides" is gaining popularity. the aims of this study were to analyze the chemical composition and assess the biological activities of hedychium essential oils. oils from 19 hedychium species and cultivars were analyzed by gas chromatography (gc) and gas chromatography-mass spectrometry (gc-ms) techniques. the antifungal and insecticidal activities of these oils were tested against colletotrichum acutatum, c ...201323579997
composition, mosquito larvicidal, biting deterrent and antifungal activity of essential oils of different plant parts of cupressus arizonica var. glabra ('carolina sapphire').essential oils obtained by hydrodistillation of female cones (fc), male cones (mc), needle-twigs (nt) and wood-bark (wb) of 'carolina sapphire' [cupressus arizonica var glabra (sudw.) little] were analyzed simultaneously by gas chromatography (gc) and gas chromatography-mass spectrometry (gc/ms). the main constituents of the investigated essential oils were as follows: fc: alpha-pinene 68.5% and myrcene 11.9%; mc: alpha-pinene 22.5%, epi-zonarene 9.9%, zonarene 6.9%, limonene 6.8% and cadina-3,5 ...201323513743
proteomic analysis of conidia germination in colletotrichum acutatum.colletotrichum acutatum is an important phytopathogenic fungus causing anthracnose in commercially important fruit crops, such as strawberry. the conidia produced by the fungus are survival structures which play a key role in host infection and fungal propagation. despite its relevance to the fungal life cycle, conidial biology has not been extensively investigated. here, we provide the first proteomic description of the conidial germination in c. acutatum by comparing the proteomic profiles of ...201323371377
application of rhizobacteria for plant growth promotion effect and biocontrol of anthracnose caused by colletotrichum acutatum on pepper.in vitro and greenhouse screening of seven rhizobacterial isolates, ab05, ab10, ab11, ab12, ab14, ab15 and ab17, was conducted to investigate the plant growth promoting activities and inhibition against anthracnose caused by colletotrichum acutatum in pepper. according to identification based on 16s rdna sequencing, the majority of the isolates are members of bacillus and a single isolate belongs to the genus paenibacillus. all seven bacterial isolates were capable of inhibiting c. acutatum to v ...201223323049
fagast2, a strawberry ripening-related gene, acts together with fagast1 to determine cell size of the fruit receptacle.numerous gast-like genes have been reported in higher plants, but only one gast-like gene (fagast1) has been described in strawberry so far. herein, we have identified a novel strawberry fagast gene (fagast2) whose expression showed an increase throughout fruit receptacle development and ripening, coinciding with those stages where a decrease in fruit expansion processes (g3-w and r-or stages) occurs. fagast2 only shares 31% and 15.7% amino acid and nucleotide sequence homology, respectively, wi ...201323231876
the colletotrichum acutatum species complex.colletotrichum acutatum is known as an important anthracnose pathogen of a wide range of host plants worldwide. numerous studies have reported subgroups within the c. acutatum species complex. multilocus molecular phylogenetic analysis (its, act, tub2, chs-1, gapdh, his3) of 331 strains previously identified as c. acutatum and other related taxa, including strains from numerous hosts with wide geographic distributions, confirmed the molecular groups previously recognised and identified a series ...201223136458
a promising strain of streptomyces sp. with agricultural traits for growth promotion and disease management.a bacterial strain, streptomyces sp. cimap- a1 was isolated from geranium rhizosphere and identified by morphological, physiological, biochemical and molecular characters (16s rdna gene sequence). phylogenetically, it was found most closely related to s. vinacendrappus, strain nrrl-2363 with 99% sequence similarity. the strain had potential antagonistic activity (in vitro) against wide range of phytopathogenic fungi like stemphylium sp., botrytis cinerea, sclerotinia sclerotiorum, colletotrichum ...201223016493
effect of temperature, wetness duration, and planting density on olive anthracnose caused by colletotrichum spp.the influence of temperature, wetness duration, and planting density on infection of olive fruit by colletotrichum acutatum and c. simmondsii was examined in laboratory and field experiments. detached olive fruit of 'arbequina', 'hojiblanca', and 'picual' were inoculated with conidia of several isolates of the pathogen and kept at constant temperatures of 5 to 35°c in humid chambers. similarly, potted plants and stem cuttings with fruit were inoculated and subjected to wetness periods of 0 to 48 ...201222957821
isolation and molecular characterization of a novel strain of bacillus with antifungal activity from the sorghum rhizosphere.we looked for bacterial strains with antifungal activity in the sorghum rhizosphere. a prescreening procedure to search for hemolytic activity among the isolated strains allowed us to detect good fungitoxic activity in a bacterial isolate that we named um96. this bacterial isolate showed strong growth inhibition in bioassays against the pathogens diaporthe phaseolorum, colletotrichum acutatum, rhizoctonia solani, and fusarium oxysporum. the supernatant of isolate um96 also showed strong hemolyti ...201222869080
bioassay-directed isolation and identification of phytotoxic and fungitoxic acetylenes from conyza canadensis.conyza canadensis (l.) cronquist syn. (horseweed) is a problematic and invasive weed with reported allelopathic properties. to identify the phytotoxic constituents of the aerial parts, a systematic bioactivity-guided fractionation of the dichloromethane extract was performed. three active enyne derivatives, (2z,8z)-matricaria acid methyl ester, (4z,8z)-matricaria lactone, and (4z)-lachnophyllum lactone, were identified. the lactones inhibited growth of the monocot agrostis stolonifera (bentgrass ...201222612410
endophytic fungi associated with leaves of betulaceae in japan.diversity and species composition of endophytic fungi on leaves of 11 tree species in betulaceae were studied, with reference to climatic, tree species, and seasonal variations. a total of 186 fungal isolates were obtained from 190 leaves collected in a subalpine forest, a cool temperate forest, and a subtropical forest in japan, and were divided into 46 operational taxonomic units (otus) according to the base sequences of d1-d2 region of large subunit rdna. the 2 most frequent otus were muscodo ...201222452312
temporal accumulation of salicylic acid activates the defense response against colletotrichum in strawberry.many authors have reported interactions between strawberry cultivars and pathogenic microorganisms, yet little is known about the mechanisms triggered in the plant. in this paper we examine the participation of the salicylic acid (sa) signaling pathway involved in the response of fragaria x ananassa cv. pájaro plants to pathogens. strawberry plants were challenged with the virulent strain m11 of colletotrichum acutatum, or with the avirulent strain m23 of colletotrichum fragariae which confers r ...201222366637
diversity and antimicrobial activities of the fungal endophyte community associated with the traditional brazilian medicinal plant solanum cernuum vell. (solanaceae).the diversity and antimicrobial activity of endophytic fungi associated with the brazilian medicinal plant solanum cernuum vell. were studied during summer and winter seasons. a total of 246 fungal isolates were obtained, including 225 filamentous fungi and 21 yeasts. they were identified by morphological, physiological, and molecular methods. fifty-five different taxa represented by the phyla ascomycota (33 taxa), basidiomycota (21 taxa), and zygomycota (one taxon) were identified. the most a ...201122182199
development and characterization of antimicrobial poly(l-lactic acid) containing trans-2-hexenal trapped in cyclodextrins.trans-2-hexenal, a naturally occurring plant volatile with antimicrobial capacity, was encapsulated into β-cyclodextrins (β-cds), enzymatically modified starch, and shown effective to control main microorganisms causing food spoilage (alternaria solani, aspergillus niger, botrytis cinerea, colletotrichum acutatum, penicillium sp). loaded β-cds were incorporated into a poly(l-lactic acid) (pla) matrix by extrusion and casting, and yielded antimicrobial polymers made from natural resources. a mast ...201122177713
isolation of a strawberry gene fragment encoding an actin depolymerizing factor-like protein from genotypes resistant to colletotrichum acutatum.actin depolymerizing factors (adfs) have been recently implicated in plant defense against pathogenic fungi, associated with the cytoskeletal rearrangements that contribute to establish an effective barrier against fungal ingress. in this work, we identified a dna fragment corresponding to a part of a gene predicted to encode an adf-like protein in genotypes of fragaria ananassa resistant to the fungus colletotrichum acutatum. bulked segregant analysis combined with aflp was used to identify pol ...201122107362
the synthetic strigolactone gr24 influences the growth pattern of phytopathogenic fungi.strigolactones that are released by plant roots to the rhizosphere are involved in both plant symbiosis with arbuscular mycorrhizal fungi and in plant infection by root parasitic plants. in this paper, we describe the response of various phytopathogenic fungi to the synthetic strigolactone gr24. when gr24 was embedded in the growth medium, it inhibited the growth of the root pathogens fusarium oxysporum f. sp. melonis, fusarium solani f. sp. mango, sclerotinia sclerotiorum and macrophomina phase ...201121688170
essential oil composition of five collections of achillea biebersteinii from central turkey and their antifungal and insecticidal activity.the composition of the essential oils hydrodistilled from the aerial parts of five achillea biebersteinii afan samples, collected in central turkey from konya, isparta and ankara, were analyzed both by gas chromatography (gc-fid) and gas chromatography-mass spectrometry (gc-ms). eighty-four components were identified, representing 87 to 99% of the total oil composition. the identified major components were 1,8-cineole (9-37%), camphor (16-30%) and p-cymene (1-27%). two samples differed in piperi ...201121615036
identification of differentially expressed genes in a resistant versus a susceptible blueberry cultivar after infection by colletotrichum acutatum.anthracnose fruit rot, caused by the fungus colletotrichum acutatum, is an important disease of blueberry worldwide. the cultivar elliott is resistant, severely restricting fungal growth and sporulation relative to the susceptible cultivar jersey. the objectives of this research were as follows: (i) to analyse pre-penetration events in 'elliott' and 'jersey' at different stages of fruit development; (ii) to identify putative defence genes in 'elliott' fruit; and (iii) to monitor the timing of th ...201121535352
effect of wetness duration and temperature on the development of anthracnose on selected almond tissues and comparison of cultivar susceptibility.blossoms, leaves, fruit, and woody tissues of almond can be affected by anthracnose caused by colletotrichum acutatum. because the disease occurs throughout rainy spring seasons, the effect of temperature and wetness duration on disease development was evaluated in controlled studies. the lowest inoculum concentration where disease developed on leaves was 104 conidia/ml. longer wetness durations were needed for leaves than for blossoms and disease increased linearly with increasing wetness durat ...201121521000
vegetative compatibility groups and parasexual segregation in colletotrichum acutatum isolates infecting different hosts.heterokaryosis is an important mechanism which provides genetic variability increase in filamentous fungi. in order to assess the diversity of vegetative compatibility reactions existing among colletotrichum acutatum isolates derived from different hosts, complementary nit mutants of each isolate were obtained and paired in all possible combinations. vegetative compatibility groups (vcg) were identified among the isolates according to their ability to form viable heterokaryons. seven vcgs were i ...201121425929
root treatment with rhizobacteria antagonistic to phytophthora blight affects anthracnose occurrence, ripening, and yield of pepper fruit in the plastic house and field.we previously selected rhizobacterial strains ccr04, ccr80, gse09, ise13, and ise14, which were antagonistic to phytophthora blight of pepper. in this study, we investigated the effects of root treatment of rhizobacteria on anthracnose occurrence, ripening, and yield of pepper fruit in the plastic house and field in 2008 and 2009. we also examined the effects of volatiles produced by the strains on fruit ripening and on mycelial growth and spore development of colletotrichum acutatum and phytoph ...201121405997
azospirillum brasilense siderophores with antifungal activity against colletotrichum acutatum.anthracnose, caused by the fungus colletotrichum acutatum is one of the most important diseases in strawberry crop. due to environmental pollution and resistance produced by chemical fungicides, nowadays biological control is considered a good alternative for crop protection. among biocontrol agents, there are plant growth-promoting bacteria, such as members of the genus azospirillum. in this work, we demonstrate that under iron limiting conditions different strains of a. brasilense produce side ...201121234749
characterization of diversity in colletotrichum acutatum sensu lato by sequence analysis of two gene introns, mtdna and intron rflps, and mating compatibility.a diverse collection of isolates identified as colletotrichum acutatum, including a range of fruit-rot and foliar pathogens, was examined for mtdna rflps and rflps and sequence variation of a 900-bp intron of the glutamine synthetase (gs) gene and a 200-bp intron of the glyceraldehyde-3-phosphate dehydrogenase (gpdh) gene. rflps of mtdna, rflps of the 900-bp gs intron and sequence analysis of each intron identified the same seven distinct molecular groups, or clades, within c. acutatum sensu lat ...200321148995
characterization of colletotrichum species associated with diseases of proteaceae.colletotrichum spp. are known to occur on and cause diseases of proteaceae, but their identities are confused and poorly understood. the aim of the present study thus was to identify accurately the colletotrichum spp. associated with diseases of cultivated proteaceae. colletotrichum spp. associated with proteaceous hosts growing in various parts of the world were identified based on morphology, sequence data of the internal transcribed spacer region (its-1, its-2), the 5.8s gene, and partial seq ...200421148951
eupatorium capillifolium essential oil: chemical composition, antifungal activity, and insecticidal activity.natural plant extracts often contain compounds that are useful in pest management applications. the essential oil of eupatorium capillifolium (dog-fennel) was investigated for antifungal and insecticidal activities. essential oil obtained by hydrodistillation of aerial parts was analyzed by gas chromatography (gc) and gas chromatography-mass spectrometry (gc/ms). the major components were determined to be thymol methyl ether (=methyl thymol) (36.3%), 2,5-dimethoxy-p-cymene (20.8%) and myrcene (1 ...201020922999
effect of 1-methylcyclopropene on anthracnose rot caused by colletotrichum acutatum and disease resistance in loquat fruit.backgound: loquat (eriobotrya japonica lindl.) fruit are susceptible to fungal decay during postharvest storage at ambient temperature. the control of postharvest diseases of loquat is based primarily on the use of synthetic fungicides. to look for an alternative method for disease control, we investigated the effect of 1-methylcyclopropene (1-mcp) on controlling anthracnose rot caused by colletotrichum acutatum of loquat fruit. results: 1-mcp treatment significantly reduced decay incidence of l ...201020648530
a gene with domains related to transcription regulation is required for pathogenicity in colletotrichum acutatum causing key lime anthracnose.summary colletotrichum acutatum causes key lime anthracnose (kla) and postbloom fruit drop (pfd) of citrus. we utilized restriction enzyme-mediated integration (remi) mutagenesis to produce six non-pathogenic mutants from a kla isolate after screening 1064 transformants on detached key lime leaves. subsequently, a gene designated klap1 (key lime anthracnose pathogenicity) was identified from one of the mutants and was demonstrated genetically to be required for pathogenicity to key lime leaves. ...200520565676
genotypic and phenotypic diversity in colletotrichum acutatum, a cosmopolitan pathogen causing anthracnose on a wide range of hosts.summary colletotrichum acutatum causes anthracnose on a wide range of hosts including woody and herbaceous crops, ornamentals, fruits and conifers. almond, citrus, lupin, olive and strawberry are some of the crops in which c. acutatum diseases are economically important. with the application of molecular markers and diagnostic pcr over the last 10-15 years, c. acutatum was identified as a major pathogen on a number of hosts instead of or along with c. gloeosporioides. c. acutatum displays high l ...200520565664
a defect in nir1, a nira-like transcription factor, confers morphological abnormalities and loss of pathogenicity in colletotrichum acutatum.summary a non-pathogenic mutant of colletotrichum acutatum, designated ca5, exhibited epiphytic hyphal growth and did not cause lesions on strawberry plants but grew necrotrophically when inoculated directly onto wounded stolons. in the absence of an external nitrogen source, the mutant exhibited extended germ-tube growth prior to appressorium formation. the deduced product of the impaired gene (nir1) is similar to nira, an aspergillus nidulans transcriptional regulator of nitrogen metabolism. i ...200620507451
effects of heat treatment on wound healing in gala and red fuji apple fruits.this study investigated the effects of heat treatment (hot air at 38 degrees c for 4 days) on wound healing in gala and red fuji apple fruits (malus domestica borkh.) and the possible mechanism. wounded apples were healed at either 20 or 38 degrees c for 4 days. during the treatment, ethylene, phenylalanine ammonia-lyase (pal), peroxidase (pod), polyphenol oxidase (ppo), hydrogen peroxide (h(2)o(2)), and phenolic and lignin contents were measured. following the treatment, healed wounds were inoc ...201020232915
gas chromatographic-mass spectrometric analysis of volatiles obtained by four different techniques from salvia rosifolia sm., and evaluation for biological activity.four different isolation techniques, conventional hydrodistillation (hd), microwave-assisted hydrodistillation (mwhd), microdistillation (md) and micro-steam distillation-solid-phase microextraction (msd-spme), have been used to analyze the volatile constituents from the aerial parts of salvia rosifolia sm. by gas chromatography and gas chromatography coupled to mass spectrometry. hd and mwhd techniques produced quantitatively (yield, 0.39% and 0.40%) and qualitatively (aromatic profile) similar ...201020015509
a new selective medium for the recovery and enumeration of monilinia fructicola, m. fructigena, and m. laxa from stone fruits.isolation of monilinia spp. from stone and pome fruit surfaces is difficult due to the presence of several fast-growing fungal species such as rhizopus, alternaria, and penicillium spp. therefore, a new selective medium (acidified potato dextrose agar [ph 3.6] amended with fosetyl-aluminum [fosetyl-al] at 500 microg/ml) (apda-f500) was developed for the recovery of monilinia propagules. the antifungal agents fosetyl-al, dichloran, ammonium molybdate, and 2-deoxy-d-glucose (2-dd-glucose) were tes ...200919740034
identification of two new genes conferring resistance to colletotrichum acutatum in capsicum baccatum.resistance to anthracnose, caused by colletotrichum capsici and c. acutatum, was investigated in capsicum baccatum pbc80 and pbc1422 and c. chinense pbc932. mature green and ripe fruit were inoculated with 13 isolates of the two colletotrichum species pbc80 contained the broadest spectrum of resistance to both colletotrichum species because none of the isolates were able to infect the genotype. at both fruit maturity stages, pbc1422 was infected by only colletotrichum acutatum. pbc932 at ripe fr ...200919671013
Displaying items 1 - 100 of 173