| dynamics of scale regeneration in seawater- and brackish water-acclimated sea bass, dicentrarchus labrax. | scale loss is a common occurrence in both wild fish and those in aquaculture production systems, and regeneration of scales has been described in several freshwater species. relatively little information exists about this process in marine fish, and in the present study, the chronology of scale regeneration was characterized in juvenile sea bass dicentrarchus labrax, maintained in full seawater (sw; 36‰, 11.2 mm ca(2+)) or brackish water (bw; 3.5‰, 1.1 mm ca(2+)). despite the significant differe ... | 2013 | 23196667 |
| concentration of polychlorinated biphenyls and organochlorine pesticides in mullet (mugil cephalus) and sea bass (dicentrarchus labrax) from bizerte lagoon (northern tunisia). | concentrations of polychlorinated biphenyls (pcbs), and organochlorine pesticides (ocps) were determined in two fish species, mullet (mugil cephalus) and sea bass (dicentrarchus labrax), collected from bizerte lagoon and the mediterranean sea. in all samples, pcbs were found in higher concentrations than ocps. the highest concentrations of ocps and pcbs were found in sea bass, and in bizerte lagoon. concentrations of ddts and pcbs detected in this study were generally comparable or slightly high ... | 2013 | 23149188 |
| diversity, molecular characterization and expression of t cell receptor γ in a teleost fish, the sea bass (dicentrarchus labrax, l). | two lineages of t cells, expressing either the αβ t cell receptor (tr) or the γδ tr, exist in gnathostomes. the latter type of t cells account for 1-10 % of t cells in blood and up to 30 % in the small intestine. they may recognize unconventional antigens (phosphorylated microbial metabolites, lipid antigens) without the need of major histocompatibility class i (mh1) or class ii (mh2) presentation. in this work we have described cloning and structural characterization of tr -chain (trg) from the ... | 2012 | 23133531 |
| molecular cloning and characterization of sea bass (dicentrarchus labrax, l.) mhc class i heavy chain and β2-microglobulin. | in this work, the gene and cdna of sea bass (dicentrarchus labrax) β2-microglobulin (dila-β2m) and several cdnas of mhc class i heavy chain (dila-ua) were characterized. while dila-β2m is single-copy, numerous dila-ua transcripts were identified per individual with variability at the peptide-binding domain (pbd), but also with unexpected diversity from the connective peptide (cp) through the 3' untranslated region (utr). phylogenetic analysis segregates dila-β2m and dila-ua into each subfamily c ... | 2013 | 23116964 |
| genotype by diet interactions in european sea bass (dicentrarchus labrax l.): nutritional challenge with totally plant-based diets. | aquaculture of carnivorous species has strongly relied on fish meal and fish oil for feed formulation; however, greater replacement by terrestrial plant-based products is occurring now. this rapid change in dietary environment has been a major revolution and has to be taken into consideration in breeding programs. the present study analyzes potential consequences of this nutritional tendency for selective breeding by estimating genetic parameters of bw and growth rates estimated by the thermal g ... | 2013 | 23100583 |
| acceleration performance of individual european sea bass dicentrarchus labrax measured with a sprint performance chamber: comparison with high-speed cinematography and correlates with ecological performance. | locomotor performance can influence the ecological and evolutionary success of a species. for fish, favorable outcomes of predator-prey encounters are often presumably due to robust acceleration ability. although escape-response or "fast-start" studies utilizing high-speed cinematography are prevalent, little is known about the contribution of relative acceleration performance to ecological or evolutionary success in a species. this dearth of knowledge may be due to the time-consuming nature of ... | 2012 | 23099467 |
| vitamin c enhances vitamin e status and reduces oxidative stress indicators in sea bass larvae fed high dha microdiets. | docosahexaenoic acid (dha) is an essential fatty acid necessary for many biochemical, cellular and physiological functions in fish. however, high dietary levels of dha increase free radical injury in sea bass (dicentrarchus labrax) larvae muscle, even when vitamin e (α-tocopherol, α-toh) is increased. therefore, the inclusion of other nutrients with complementary antioxidant functions, such as vitamin c (ascorbic acid, vitc), could further contribute to prevent these lesions. the objective of th ... | 2012 | 23086553 |
| daily rhythms of blood glucose differ in diurnal and nocturnal european sea bass (dicentrarchus labrax l.) undergoing seasonal phase inversions. | sea bass change their feeding rhythms from diurnal to nocturnal in winter, returning to diurnal feeding in spring. despite behavioral data, the physiological changes that take place during such changes remain unexplored. in this paper, blood glucose rhythms of european sea bass with diurnal/nocturnal self-feeding rhythms were investigated during phase inversions of their feeding behavior (in winter and spring) when both diurnal and nocturnal fish coexist. blood glucose showed daily variations in ... | 2013 | 23053614 |
| kisspeptin and seasonal control of reproduction in male european sea bass (dicentrarchus labrax). | in the present study, we developed and validated real-time quantitative rt-pcr assays for a suite of genes involved in the brain-pituitary gonadal axis in fish including kisspeptin genes and its receptor (kiss1, kiss2, kissr4) and gonadotropin-releasing hormone genes (sbgnrh, sgnrh, cgnrhii) in the brain, and gonadotropin genes (fshβ and lhβ) in the pituitary. sex steroid profiles (t and 11-kt) and gonadal development were also studied over a full annual reproductive cycle in adult male sea bass ... | 2012 | 23036731 |
| trace elements in two marine fish species during estuarine residency: non-essential versus essential. | trace element levels in fish are of particular interest, owing the potential risk to human health. in accordance, juveniles of dicentrarchus labrax and of liza aurata were sampled and arsenic, cadmium, chromium, selenium and zinc were determined in the muscle. the levels of trace elements in muscle demonstrated to be similar for both species and sites, with the exception of selenium levels at reference, which seemed to be higher in d. labrax. moreover, apart from arsenic levels in muscle, all el ... | 2012 | 23017951 |
| effect of water temperature increase on ho-1 expression in european sea bass (dicentrarchus labrax l.) tissues. | one of the most pertinent environmental factors influencing the marine organism life is temperature. it has been demonstrated that an increase of temperature is able to induce the synthesis of heat shock proteins (hsp). in this study we investigated the expression of ho-1 mrna, also referred to as hsp32, in different tissues of european sea bass (dicentrarchus labrax, l.) at several time points after increased temperature exposure (from 12degc to 30degc). our results showed that ho-1 was not exp ... | 2012 | 22992441 |
| nodavirus infection induces a great innate cell-mediated cytotoxic activity in resistant, gilthead seabream, and susceptible, european sea bass, teleost fish. | viral nervous necrosis (vnn) virus produces great mortalities in fish having susceptible and reservoir species between the most important marine aquaculture species. cell-mediated cytotoxicity (cmc) is considered, towards the interferon (ifn), the most important mechanism of the immune response to fight against viral infections but it has been very scarcely evaluated. we aimed to evaluate the effects of vnnv infection in the reservoir gilthead seabream (sparus aurata) and susceptible european se ... | 2012 | 22981914 |
| mercury-induced chromosomal damage in wild fish (dicentrarchus labrax l.) reflecting aquatic contamination in contrasting seasons. | ria de aveiro (mainly laranjo basin, portugal) has been subjected to mercury contamination from a chlor-alkali plant, currently presenting a well-described mercury gradient. this study aimed to assess mercury genotoxicity in this area by measuring the frequency of erythrocytic nuclear abnormalities (ena) in the european sea bass (dicentrarchus labrax), addressing the relation with total mercury concentration in the blood and the modulatory role of seasonal variables. fish were collected, in warm ... | 2012 | 22968208 |
| quality of farmed and wild sea bass lipids studied by (1)h nmr: usefulness of this technique for differentiation on a qualitative and a quantitative basis. | proton nuclear magnetic resonance ((1)h nmr) spectroscopy was employed to study the lipids of farmed and wild european sea bass (dicentrarchus labrax). this technique provided a great deal of detailed information on sea bass lipids composition and, once the spectra signals have been assigned, the simple observation of the spectra enables one to distinguish cultured from wild fish. this distinction is possible due to the presence in the former of high proportions of diunsaturated acyl groups, mai ... | 2012 | 22953897 |
| characterisation and expression during sex differentiation of sox19 from the sea bass dicentrarchus labrax. | the sox family of transcription factors are involved in a variety of developmental processes including sex determination and gonadal differentiation. sox19 is a particularly interesting member of this family that has been found only in fish, though mammals have a very diverged orthologue that is designated sox15 and assigned to a different sox family subgroup. here we describe the cloning and characterisation of sox19 from the european sea bass (dicentrarchus labrax), an important aquaculture sp ... | 2012 | 22940139 |
| expression of kisspeptins in the brain and pituitary of the european sea bass (dicentrarchus labrax). | kisspeptins are now considered key players in the neuroendocrine control of puberty and reproduction, at least in mammals. most teleosts have two kiss genes, kiss1 and kiss2, but their sites of expression are still poorly documented. as a first step in investigating the role of kisspeptins in the european sea bass, a perciform fish, we studied the distribution of kiss1 and kiss2-expressing cells in the brain of males and females undergoing their first sexual maturation. animals were examined at ... | 2013 | 22886357 |
| cloning and expression analysis of follicle-stimulating hormone and luteinizing hormone receptor during the reproductive cycle in korean rockfish (sebastes schlegeli). | full-length cdna sequences encoding the receptors for follicle-stimulating hormone (fshr) and luteinizing hormone (lhr) were isolated from ovary of korean rockfish (sebastes schlegeli) using reverse transcription-polymerase chain reaction (pcr) and rapid amplification of cdna ends procedures. the cdna of the krfshr encodes a predicted protein of 703 amino acids that showed the greatest homology with european seabass (dicentrarchus labrax) (78 %) and gilthead seabream (sparus aurata) (73 %). the ... | 2013 | 22843313 |
| molecular cloning, tissue distribution and daily expression of cry1 and cry2 clock genes in european seabass (dicentrarchus labrax). | biological rhythms are driven by circadian oscillators, which are ultimately controlled by the cyclic expression of clock genes. cryptochromes (cry), blue light photoreceptors, belong to the negative elements of the transcriptional feedback loop into the molecular clock. this paper describes the cloning and characterization of two cryptochromes (cry1 and 2) in european seabass, which is considered an interesting chronobiology model due to its dual (diurnal/nocturnal) behavior. the cloned cdna fr ... | 2012 | 22841604 |
| development of an in vitro system for functional studies of ovarian follicular cells in european sea bass (dicentrarchus labrax). | the layers of follicular cells surrounding the oocyte and the interactions among them and the germ cells are critical for the successful maintenance of the ovarian functions. we have set up the isolation procedure and culture conditions of sea bass ovarian follicular cells. their behaviour at three different physiological temperatures (25, 18 and 15 °c) was evaluated by verifying their steroidogenic capacity along time together with the expression of follicular specific genes (cyp19a1, fshr, lhr ... | 2013 | 22760552 |
| osmoregulatory response to low salinities in the european sea bass embryos: a multi-site approach. | embryonic osmoregulation effected by embryonic ionocytes in the european sea bass dicentrarchus labrax has been studied at several sites, including the yolk sac membrane, the first gill slits and the gut ionocytes. d. labrax embryos, spawned in seawater (sw) (39 ‰), were exposed to dilute seawater (dsw) (5 ‰) during 48 h, from stage 10 pairs of somites (10s) to hatching time (ht). control embryos originating from the same spawn were maintained in sw. both sw and dsw embryos were examined after 2 ... | 2013 | 22752053 |
| short-term exposure of the european sea bass dicentrarchus labrax to copper-based antifouling treated nets: copper bioavailability and biomarkers responses. | we studied if the levels of copper released from antifouling treated nets used in finfish mariculture could affect the immune defense mechanism and/or induce oxidative stress in dicentrarchus labrax, after short term exposure in laboratory experiments. dissolved copper concentration released from the treated nets, copper bioavailability and a set of biomarkers responses were measured. biomarkers included hemoglobin concentration, activities of lysozyme, total complement, respiratory burst, gluta ... | 2012 | 22698372 |
| differentiation of fresh and frozen/thawed fish, european sea bass (dicentrarchus labrax), gilthead seabream (sparus aurata), cod (gadus morhua) and salmon (salmo salar), using volatile compounds by spme/gc/ms. | a simple method based on solid phase microextraction/gas chromatography/mass spectrometry (spme/gc/ms) was applied for studying the volatile profiles of whole fish samples of european sea bass (dicentrarchus labrax) and gilthead seabream (sparus aurata) and fillets of cod (gadus morhua) and salmon (salmo salar) during frozen storage in order to be able to differentiate a fresh product from one that has been frozen. analysis of volatile compounds was performed on these two product types, fresh an ... | 2012 | 22641336 |
| rodlet cells development in the intestine of sea bass (dicentrarchus labrax). | the enigmatic rodlet cells (rcs) are characterized by conspicuous inclusions named "rodlets". they were discovered over 100 years ago and were considered as parasites but shortly afterward interpreted as endogenous cells. the rcs have been described in different tissues of marine and freshwater teleosts, but their origin and function remain unknown. this work was designed to an ultrastructural study on rcs development and distribution in intestinal epithelium of dicentrarchus labrax. three diffe ... | 2012 | 22622998 |
| hepatic glycogen synthesis in farmed european seabass (dicentrarchus labrax l.) is dominated by indirect pathway fluxes. | hepatic glycogen synthesis fluxes from direct and indirect pathways were quantified in seabass by postmortem (2)h nmr analysis of plasma water (pw) and glycogen glucosyl (2)h enrichments from (2)h-enriched seawater. eighteen fish (28.0 ± 1.7 cm and 218.0 ± 43.0 g) were divided into three groups of 6 and studied over 24 days with transfer to 5% (2)h-seawater after day 21. over this period, one group was fed daily with fishmeal, a second group was fasted, and a third group was fasted for 21 days f ... | 2012 | 22561667 |
| 2d dige/ms to investigate the impact of slaughtering techniques on postmortem integrity of fish filet proteins. | two-dimensional difference gel electrophoresis (2d dige) was applied to investigate the impact of slaughtering on the postmortem integrity of muscle tissue proteins in european sea bass (dicentrarchus labrax). three different slaughtering techniques were evaluated: asphyxia in air (aa), asphyxia in ice (ai), and spinal cord severance (scs). principal components analysis (pca) revealed a significant divergence of scs samples, whereas aa and ai samples, although grouped separately, were less diver ... | 2012 | 22561645 |
| seasonal screening of ache, gsh and gonad histology, in european sea bass dicentrarchus labrax l. reared in three different fish farms. | the aim of this work was to do a preliminary seasonal screening of ecotoxicological biomarkers in european sea bass dicentrarchus labrax in three different fish farms, to know if the different location and typology can discriminate them. a set of selected biomarkers of xenobiotic exposure, such as acetylcholinesterase (ache) activity, glutathione (gsh) and gonad morphology were investigated seasonally in male european sea bass d. labrax (l.) reared in three different intensive farms: a land-base ... | 2013 | 22533608 |
| heritability of cortisol response to confinement stress in european sea bass dicentrarchus labrax. | in fish, the most studied production traits in terms of heritability are body weight or growth, stress or disease resistance, while heritability of cortisol levels, widely used as a measure of response to stress, is less studied. in this study, we have estimated heritabilities of two growth traits (body weight and length) and of cortisol response to confinement stress in the european sea bass. | 2012 | 22520515 |
| morphology of the european sea bass (dicentrarchus labrax) tongue. | the european sea bass, a member of the moronidae family, is a food fish, considered one of the first models for the intensive breeding in salt water. it has nowadays an important and increasing presence in the international fishing markets. sea basses are carnivorous, feeding on little fishes and invertebrates. considering the important role of the tongue during the intraoral transport and the swallowing of food, scarce data are present in literature about its morphology. the aim of this study w ... | 2012 | 22505185 |
| a monoclonal antibody for the cd45 receptor in the teleost fish dicentrarchus labrax. | the cd45 tyrosine phosphatase plays an important role in regulating t lymphocyte activation in vertebrate species. in this study we describe some molecular and functional features of the cd45 receptor molecule from the european sea bass dicentrarchus labrax. following immunization with fixed sea bass thymocytes, we obtained a murine monoclonal antibody (mab) able to stain fish leucocytes both alive, by immunofluorescence of thymus and mucosal tissues, and fixed, by in situ immunohistochemistry o ... | 2012 | 22504161 |
| sea bass sperm freezability is influenced by motility variables and membrane lipid composition but not by membrane integrity and lipid peroxidation. | cryopreserved sperm quality depends on the characteristics of fresh sperm. thus, it is necessary to establish a group of variables to predict the cryopreservation potential of the fresh samples with the aim of optimizing resources. motility, viability, lipid peroxidation and lipid profile of european sea bass (dicentrarchus labrax) sperm were determined before and after cryopreservation to establish which variables more accurately predict the sperm cryopreservation potential in this species. cry ... | 2012 | 22503480 |
| screening of ecotoxicological, qualitative and reproductive variables in male european sea bass dicentrarchus labrax (l.) reared in three different fish farms: facility location and typology. | the aim of this study was to investigate the influence of both facility location and typology of fish farm on some ecotoxicological, qualitative and reproductive variables in european sea bass dicentrarchus labrax l. several variables were investigated: gonado-somatic index (gsi), liver-somatic index (lsi); 7-ethoxyresorufin-o-deethylase (erod), benzo(a)pyrene monooxygenase and acetylcholinesterase activities; glutathione (gsh), testosterone, 17β-estradiol, total lipid, phospholipid (pl) and tri ... | 2013 | 22489960 |
| characteristics of fads2 gene expression and putative promoter in european sea bass (dicentrarchus labrax): comparison with salmonid species and analysis of cpg methylation. | marine fish species exhibit low capacity to biosynthesise highly unsaturated fatty acid (hufa) in comparison to strict freshwater and anadromous species. it is admitted that the delta(6) desaturase (fads2) is a key enzyme in the hufa biosynthetic pathway. we investigated by quantitative pcr the relative amounts of fads2 mrna in european sea bass (dicentrarchus labrax) in comparison with a salmonid species, the rainbow trout (oncorhynchus mykiss l.). the analysis of the expression data was perfor ... | 2012 | 22325717 |
| responses of juvenile sea bass, dicentrarchus labrax, exposed to acute concentrations of crude oil, as assessed by molecular and physiological biomarkers. | in the present study, juvenile sea bass were exposed for 48 and 96 h to an arabian light crude oil and their responses were assessed at the molecular and physiological levels. the aim of the study was therefore to assess (i) the short term effects of crude oil exposure by the measurement of several molecular biomarkers, (ii) the consequences of this short term exposure on fish health by using growth and condition indices measured after a decontamination period of 28 and 26 d in seawater. hydroca ... | 2012 | 22236592 |
| use of near-infrared spectroscopy for fast fraud detection in seafood: application to the authentication of wild european sea bass (dicentrarchus labrax). | the possibility of using near-infrared spectroscopy (nirs) for the authentication of wild european sea bass ( dicentrarchus labrax ) was investigated in this study. three different chemometric techniques to process the nir spectra were developed, and their ability to discriminate between wild and farmed sea bass samples was evaluated. one approach used spectral information to directly build the discrimination model in a latent variable space; the second approach first used wavelets to transform ... | 2012 | 22224758 |
| a phylogeny of the temperate seabasses (moronidae) characterized by a translocation of the mt-nd6 gene. | the entire mitochondrial genome of the striped bass morone saxatilis was sequenced together with the mitochondrial (mt) control regions of the white bass morone chrysops, white perch morone americana, yellow bass morone mississippiensis, spotted seabass dicentrarchus punctatus, european seabass dicentrarchus labrax and the japanese seabass lateolabrax japonicus. the resultant 17 580 base pair circular genome of m. saxatilis contains 38 genes (13 proteins, 23 transfer rnas and two ribosomal rnas) ... | 2012 | 22220893 |
| oxidative stress, genotoxicity and histopathology biomarker responses in mullet (mugil cephalus) and sea bass (dicentrarchus labrax) liver from bizerte lagoon (tunisia). | the aim of the study was to evaluate the impact of environmental contaminants on oxidative stress, genotoxic and histopathologic biomarkers in liver of mullet (mugil cephalus) and sea bass (dicentrarchus labrax) collected from a polluted coastal lagoon (bizerte lagoon) in comparison to a reference site (the mediterranean sea). antioxidant enzyme activities were lower in fish from the polluted site compared with fish from the reference site, suggesting deficiency of the antioxidant system to comp ... | 2012 | 22206722 |
| impact of acute stress on antimicrobial polypeptides mrna copy number in several tissues of marine sea bass (dicentrarchus labrax). | in comparison to higher vertebrates, fish are thought to rely heavily on innate immune system for initial protection against pathogen invasion because their acquired immune system displays a considerably poor immunological memory, and short-lived secondary response. the endogenous antimicrobial polypeptides (ampps) directly and rapidly killing pathogens such as bacteria, fungi, parasites, and viruses are included within the realm of innate defenses. in addition to piscidins, ampps that in recent ... | 2011 | 22204309 |
| lernanthropus kroyeri (van beneden and hesse 1851) parasitic copepoda (siphonostomatoidae, lernanthropidae) of european cultured sea bass dicentrarchus labrax (linnaeus 1758) from corsica: ecological and morphological study. | lernanthropus kroyeri (copepoda, siphonostomatoida: lernanthropidae) is a gill parasite found on the european sea bass dicentrarchus labrax. during a survey of sea bass of corsican fish farms, we studied the biology of this parasite under culture conditions. we first chose to conduct a scanning electron microscopic study to give additional information about the lifestyle of the parasite. our examinations made it possible to reveal some unreported superficial structures including details not desc ... | 2012 | 22179262 |
| challenges in assessing the toxic effects of polycyclic aromatic hydrocarbons to marine organisms: a case study on the acute toxicity of pyrene to the european seabass (dicentrarchus labrax l.). | the acute toxicity (96 h) of pyrene (py) to european seabass (dicentrachus labrax) juveniles assessed in a semi-static bioassay (ssb) with medium renewal at each 12h, and in a static bioassay (sb) without medium renewal was compared in laboratorial conditions (water py concentrations: 0.07-10 mg l(-1)). main findings in the ssb that assessed mainly the toxicity of py and its metabolites were: increased levels of bile py metabolites in good agreement with the profile of lipid peroxidation levels ... | 2012 | 22154001 |
| incorporation of ascorbic acid and α-tocopherol to the extender media to enhance antioxidant system of cryopreserved sea bass sperm. | despite the overwhelming application of sperm cryopreservation in aquaculture and broodstock management, its detrimental effects on sperm quality must be taken into account. imbalance of reactive oxygen species is considered one of the main triggers of cell damage after cryopreservation, because the spermatozoa antioxidant system is decimated during this process, mainly because the natural antioxidants present in seminal plasma diminish when sperm is diluted in extenders. it has been demonstrate ... | 2012 | 22153272 |
| the retina is a target for gnrh-3 system in the european sea bass, dicentrarchus labrax. | the european sea bass expresses three gnrh (gonadotrophin releasing hormone) forms that exert pleiotropic actions via several classes of receptors. the gnrh-1 form is responsible for the endogenous regulation of gonadotrophin release by the pituitary gland but the role of gnrh-2 and gnrh-3 remains unclear in fish. in a previous study performed in sea bass, we have provided evidence of direct links between the gnrh-2 cells and the pineal organ and demonstrated a functional role for gnrh-2 in the ... | 2012 | 22138555 |
| multicentric infiltrative lipoma in a farmed mediterranean seabass dicentrarchus labrax: a pathological and biochemical case study. | a tumour diagnosed as multicentric infiltrative lipoma affecting a single farmed seabass is described. the fish had 3 masses on the lateral side of its back, deforming the skin surface. the masses showed a tendency to invade the underlying musculature. histologically, the neoplasm consisted of differentiated adipocytes. biochemically, the neoplastic tissue showed lower values of monounsaturated fatty acids and higher values of polyunsaturated fatty acids compared with adjacent normal muscular ti ... | 2011 | 22132504 |
| trace elements and metals in farmed sea bass and gilthead bream from tenerife island, spain. | the aim of this study was to determine the levels of metals (ca, k, na, mg) and trace metals (ni, fe, cu, mn, zn, pb, cd) in two fish species (gilthead bream [sparus aurata] and sea bass [dicentrarchus labrax]) collected from fish farms located along the coast of tenerife island. ca, k, na, mg, fe, cu, zn, and mn were measured by flame atomic absorption spectrometry, whereas pb, cd, and ni were determined using graphite furnace atomic absorption spectrometry. mean fe, cu, mn, and zn contents wer ... | 2011 | 22054198 |
| effects of water temperature increase and heavy metals contamination on wap65 gene expression in sea bass (dicentrarchus labrax) liver. | it has been previously demonstrated that "warm temperature acclimation-related 65 kd protein" (wap65) is involved in temperature acclimation, response to intoxication and infection, as well as in development. the expression of wap65-1 was investigated in the liver of european sea bass (dicentrarchus labrax) during exposure to the increased temperature (from 12 deg c to 30 deg c) and during intoxication with four heavy metals: lead, cadmium, copper and zinc. post temperature increase wap65 expres ... | 2011 | 22047796 |
| fish consumption and risk of contamination by mercury---considerations on the definition of edible parts based on the case study of european sea bass. | in the present study, the risk to humans by consuming european sea bass (dicentrarchus labrax), captured at three sites along a hg contamination gradient, was evaluated by comparing muscle and kidney total hg (t-hg) levels with the european regulations for marketed fish. moreover, t-hg and organic hg (o-hg) levels in muscle were compared with the provisional tolerable weekly intake (ptwi) and the reference dose (rfd). although t-hg levels in muscle were below the european value allowable for mar ... | 2011 | 22041498 |
| impact of ultraviolet-b radiation on planktonic fish larvae: alteration of the osmoregulatory function. | coastal marine ecosystems are submitted to variations of several abiotic and biotic parameters, some of them related to global change. among them the ultraviolet-b (uv-b) radiation (uvbr: 280-320 nm) may strongly impact planktonic fish larvae. the consequences of an increase of uvbr on the osmoregulatory function of dicentrarchus labrax larvae have been investigated in this study. in young larvae of d. labrax, as in other teleosts, osmoregulation depends on tegumentary ion transporting cells, or ... | 2012 | 22018917 |
| effects of the total replacement of fish-based diet with plant-based diet on the hepatic transcriptome of two european sea bass (dicentrarchus labrax) half-sibfamilies showing different growth rates with the plant-based diet. | efforts towards utilisation of diets without fish meal (fm) or fish oil (fo) in finfish aquaculture have been being made for more than two decades. metabolic responses to substitution of fishery products have been shown to impact growth performance and immune system of fish as well as their subsequent nutritional value, particularly in marine fish species, which exhibit low capacity for biosynthesis of long-chain poly-unsaturated fatty acids (lc-pufa). the main objective of the present study was ... | 2011 | 22017880 |
| toxic effects of harmful benthic dinoflagellate ostreopsis ovata on invertebrate and vertebrate marine organisms. | harmful benthic microalgae blooms are an emerging phenomenon causing health and economic concern, especially in tourist areas. this is the case of the mediterranean sea, where ostreopsis ovata blooms occur in summer, with increasing regularity. ostreopsis species produce palytoxin (ptx) and analogues, and a number of deaths directly associated with the ingestion of ptx contaminated seafood have been reported. ptx is considered one of the most toxic molecules occurring in nature and can provoke s ... | 2012 | 22000703 |
| effects of "in vivo" exposure to toxic sediments on juveniles of sea bass (dicentrarchus labrax). | aquatic ecosystems are affected by all the impacts generated by a variety of anthropogenic activities present along coastal environments. the sediment compartment is the final receptor of water-insoluble pollutants, acting both as a sink and as a source of pollutants to the water column, and affecting both nektonic and benthic organisms. the aim of this study is to assess the impact of metals in the sediments collected from two sites in the petrochemical area between augusta and priolo (sr, sici ... | 2011 | 21996255 |
| polybrominated diphenyl ethers and their methoxylated analogs in mullet (mugil cephalus) and sea bass (dicentrarchus labrax) from bizerte lagoon, tunisia. | concentrations of ten polybrominated diphenyl ethers (pbdes) and eight methoxylated polybrominated diphenyl ethers (meo-pbdes) in mullet (mugil cephalus) and sea bass (dicentrarchus labrax) collected from the bizerte lagoon and the mediterranean sea were investigated. to the best of our knowledge, this is the first report of these compounds in marine fishes from tunisia. the pbde mean concentrations in fish from bizerte lagoon were 45.3 and 96.2 ng g(-1) lw respectively in mullet and sea bass, w ... | 2011 | 21983038 |
| bone morphogenetic protein 15 and growth differentiation factor 9 expression in the ovary of european sea bass (dicentrarchus labrax): cellular localization, developmental profiles, and response to unilateral ovariectomy. | vertebrate oocytes actively contribute to follicle development by secreting a variety of growth factors, among which bone morphogenetic protein 15 (bmp15/bmp15) and growth differentiation factor 9 (gdf9/gdf9) have been paid particular attention. in the present study, we describe the cellular localization, the developmental profiles, and the response to unilateral ovariectomy (a procedure implying the surgical removal of one of the ovaries) of protein and mrna steady-state levels of bmp15 and gdf ... | 2011 | 21978589 |
| modulation of cyp1a and genotoxic effects in european seabass (dicentrarchus labrax) exposed to weathered oil: a mesocosm study. | the aim of this study was to assess medium-term toxicity of weathered oil on european seabass. a mesocosm system reproducing an oil spill at sea was applied. fish were collected after 48 h, 7, 30 and 60 days. cyp1a gene transcription, erod and udpgt activities, bile pahs metabolites and micronuclei frequency were investigated. a progressive disappearance of low molecular weight n-alkanes and pahs in the water of the mesocosm occurred during the experimentation. fishes exposed to oil displayed a ... | 2012 | 21963215 |
| assessing food web dynamics and relative importance of organic matter sources for fish species in two portuguese estuaries: a stable isotope approach. | stable carbon and nitrogen isotopes (δ¹³c, δ¹⁵n) were used to analyse food web dynamics of two of the main estuaries of the portuguese coast: tejo and mira. the ultimate sources of organic matter supporting production of some of the most abundant and commercially important fish species were determined; and seasonal, inter- and intra- estuarine differences in the trophic relations among producers and consumers were identified. stable isotope analysis was performed in different producers, primary ... | 2011 | 21958908 |
| seasonal effects on hematological and innate immune parameters in sea bass dicentrarchus labrax. | the temperate aquatic environment is affected by two primary components of season, temperature and photoperiod, during the annual cycle. many organisms respond to seasonal change physiologically, behaviorally or both. the aim of this study was to investigate the effect of seasonality on cortisol, hematological and innate immune parameters in european sea bass reared under traditional semi-intensive aquaculture. sea bass (dicentrarchus labrax) were reared in an outdoor pond and serum cortisol, he ... | 2011 | 21945635 |
| afferent and efferent connections of the pineal organ in the european sea bass dicentrarchus labrax: a carbocyanine dye tract-tracing study. | the pineal organ of fish is a photosensitive structure that receives light information from the environment and transduces it into hormonal (rhythmic melatonin secretion) and neural (efferent projections/neurotransmitters) signals. in this study, we focused on this neural output. thus, we performed a tract-tracing study using 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (dii), a fluorescent carbocyanine dye, in order to elucidate the efferent and afferent connections of the ... | 2011 | 21921581 |
| whole body cortisol and expression of hsp70, igf-i and mstn in early development of sea bass subjected to heat shock. | whole body cortisol levels were determined during early larval developmental stages of sea bass (dicentrarchus labrax) subjected to a heat shock with the aim to investigate the correlation between the stress event and the activation of the hypothalamic-pituitary-interrenal axis. moreover, the mrna expression of inducible heat shock protein 70 (hsp70), insulin-like growth factor i (igf-i) and myostatin (mstn) was also detected. whole body cortisol was determined by a radio-immunoassay (ria) techn ... | 2011 | 21872596 |
| thyroid dysfunction in sea bass (dicentrarchus labrax): underlying mechanisms and effects of polychlorinated biphenyls on thyroid hormone physiology and metabolism. | the current study examines the effect of subchronic exposure to a mixture of aroclor standards on thyroid hormone physiology and metabolism in juvenile sea bass. the contaminant mixture was formulated to reflect the persistent organic pollution to which the european sea bass population could conceivably be exposed (0.3, 0.6 and 1.0 μg σ7pcbs per g food pellets) and higher (10 μg σ7pcbs per g food pellets). after 120 days of exposure, histomorphometry of thyroid tissue, muscular thyroid hormone c ... | 2011 | 21872555 |
| behavioural and kinematic components of the fast-start escape response in fish: individual variation and temporal repeatability. | inter-individual variation in physiological performance traits, which is stable over time, can be of potential ecological and evolutionary significance. the fish escape response is interesting in this regard because it is a performance trait for which inter-individual variation may determine individual survival. the temporal stability of such variation is, however, largely unexplored. we quantified individual variation of various components of the escape response in a population of european sea ... | 2011 | 21865523 |
| effects of carbofuran on the sea bass (dicentrarchus labrax l.): study of biomarkers and behaviour alterations. | the objective of this study was to investigate the acute effects of the pesticide carbofuran on the sea bass (dicentrarchus labrax) using parameters at different levels of biological organisation (swimming behaviour and several biomarkers) and possible relationships between alterations found in different effect criteria. in a bioassay, sea bass juveniles were individually exposed to different doses of carbofuran (31, 63, 125 and 250 μg/l) for 96 h. at the end of the bioassay, the swimming perfor ... | 2011 | 21864905 |
| first record of anisakid juveniles (nematoda) in the european seabass dicentrarchus labrax (family: moronidae), and their role as bio-indicators of heavy metal pollution. | this study assessed the anisakid nematode distribution pattern in the fish collected from coasts of mediterranean sea, egypt, during the period september 2010-april 2011. two hundred thirty out of 300 (76.7%) dicentrarchus labrax (european seabass) marine fishes belonging to family moronidae were dissected and found to be infected with larva three nematodes. the larvae had been studied by light and scanning electron microscopy. the present work represents the first record of the presence of the ... | 2012 | 21845410 |
| effect of an experimental oil spill on vertebral bone tissue quality in european sea bass (dicentrarchus labrax l.). | in order to identify biomarkers of oil pollution in fish we tested the effects of an experimental light cycle oil (lco) exposure on vertebral bone of sea bass, dicentrarchus labrax l. a total of 60 adult fish were acclimated for fifteen days, then twenty were collected as controls (day 0) while 40 were exposed to a soluble fraction of lco (1136 ng l(-1) of ten polycyclic aromatic hydrocarbons, pahs) for seven days. twenty of them were sampled at the end of the exposure period and the twenty last ... | 2011 | 21831432 |
| expression and localization of aquaporin 1a in the sea-bass (dicentrarchus labrax) during ontogeny. | the successful establishment of a species in a given habitat depends on the ability of each of its developing stages to adapt to the environment. in order to understand this process we have studied the adaptation of a euryhaline fish, the sea-bass dicentrarchus labrax, to various salinities during its ontogeny. the expression and localization of aquaporin 1a (aqp1a) mrna and protein were determined in different osmoregulatory tissues. in larvae, the sites of aqp1a expression are variable and the ... | 2011 | 21808622 |
| purification and characterization of carbonic anhydrase from the teleost fish dicentrarchus labrax (european seabass) liver and toxicological effects of metals on enzyme activity. | carbonic anhydrase (ec 4.2.1.1; ca) was purified and characterized from the liver of the teleost fish dicentrarchus labrax (european seabass) for the first time. the purification procedure consisted of a single step affinity chromatography on sepharose 4b-tyrosine-sulfanilamide. the enzyme was purified 78.8-fold with a yield of 46%, and a specific activity of 751.72u/mg proteins. it has an optimum ph at 7.5; an optimum temperature at 25°c; an optimum ionic strength at 10mm and a stable ph at 8.5 ... | 2011 | 21787732 |
| targeted gene expression profiling in european sea bass (dicentrarchus labrax, l.) follicles from primary growth to late vitellogenesis. | a real-time pcr-based gene expression survey was performed on isolated european sea bass follicles from primary growth to late vitellogenesis. expression levels of 18 transcripts with demonstrated relevance during oogenesis, encoding gonadotropin, thyrotropin, estrogen, androgen, and vitellogenin receptors, steroidogenesis-related as well as growth and transcription factors were measured. primary oocytes showed high mrna levels of insulin-like growth factors 1 and 2, bone morphogenetic protein 4 ... | 2011 | 21782032 |
| analysis of glucose metabolism in farmed european sea bass (dicentrarchus labrax l.) using deuterated water. | glucose metabolism in free-swimming fasted and fed seabass was studied using deuterated water ((2)h(2)o). after transfer to seawater enriched with 4.9% (2)h(2)o for 6-h or for 72-h, positional and mole percent enrichment (mpe) of plasma glucose and water were quantified by (2)h nmr and esi-ms/ms. plasma water (2)h-enrichment reached that of seawater within 6h. in both fasted and fed fish, plasma glucose mpe increased asymptotically attaining ~55% of plasma water enrichment by 72 h. the distribut ... | 2011 | 21777686 |
| in vivo effects of the soluble fraction of light cycle oil on immune functions in the european sea bass, dicentrarchus labrax (linné). | hydrocarbons are major contaminants that may affect biota at various trophic levels in estuaries and coastal ecosystems. the effects of accidental pollution by light cycle oil (lco), a refined product of heavy fuel oil, on bioaccumulation, depuration processes and immune-related parameters in the european sea bass, dicentrarchus labrax, were investigated in the laboratory after 7 days of exposure and a 2-week recovery period. exposure of fish to the soluble fraction of lco (1600ngl(-1)) for 7 da ... | 2011 | 21764455 |
| determination of fsh quantity and bioactivity during sex differentiation and oogenesis in european sea bass. | follicle-stimulating hormone (fsh) is a glycoprotein hormone that plays a key role in the regulation of gonadal functions in vertebrates. the present study reports the monitoring of pituitary and plasma fsh levels during sex differentiation and oogenesis in european sea bass (dicentrarchus labrax) using a homologous immunoassay and an in vitro bioassay. both assays were used complementarily for the first time in a fish species. high levels of fsh bioactivity in plasma were found during the initi ... | 2011 | 21715715 |
| directed sequencing and annotation of three dicentrarchus labrax l. chromosomes by applying sanger- and pyrosequencing technologies on pooled dna of comparatively mapped bac clones. | dicentrarchus labrax is one of the major marine aquaculture species in the european union. in this study, we have developed a directed-sequencing strategy to sequence three sea bass chromosomes and compared results with other teleosts. three bac dna pools were created from sea bass bac clones that mapped to stickleback chromosomes/groups v, xvii and xxi. the pools were sequenced to 17-39x coverage by pyrosequencing. data assembly was supported by sanger reads and mate pair data and resulted in s ... | 2011 | 21693181 |
| metallothioneins failed to reflect mercury external levels of exposure and bioaccumulation in marine fish--considerations on tissue and species specific responses. | the suitability of metallothioneins (mt) in fish as biomarker of exposure to mercury has been questioned. therefore, this study aimed at investigating the relationship between external levels of exposure, mercury accumulation and mt content, assessing species and tissue specificities. two ecologically different fish species--dicentrarchus labrax and liza aurata--were surveyed in an estuary historically affected by mercury discharges. total mercury (t-hg) and mt content were determined in gills, ... | 2011 | 21680008 |
| effects of postmortem storage temperature on sea bass (dicentrarchus labrax) muscle protein degradation: analysis by 2-d dige and ms. | storage conditions are known to be important for postmortem deterioration of fish muscle, and temperature is one of the factors with the strongest impact on this process. in order to shed light on the influence of temperature on the status of sea bass (dicentrarchus labrax) muscle proteins during postmortem storage, a 2-d dige and mass spectrometry study was performed on fish kept at either 1 or 18°c for 5 days. as expected, the greatest alterations in sea bass filet protein composition were obs ... | 2011 | 21656684 |
| single and combined genotoxic and cytotoxic effects of two xenobiotics widely used in intensive aquaculture. | several chemicals are used in aquaculture to prevent or to treat disease outbreaks. these substances are mainly administered by two different routes: by prolonged immersion or by mixing into the diet. in the case of intensive aquaculture, the chemicals that are most frequently applied by immersion are formaldehyde (fa) 37% and oxytetracycline (otc). the first is highly effective against most protozoa, as well as some of the most common parasites such as monogenetic trematodes. otc presents a lar ... | 2011 | 21621636 |
| mechanisms of oocyte development in european sea bass (dicentrarchus labrax l.): investigations via application of unilateral ovariectomy. | unilateral ovariectomy (ulo) was performed in european sea bass (dicentrarchus labrax l.) during late pre-vitellogenesis/early vitellogenesis. plasma steroid levels and the expression of a suite of potential oogenesis-relevant genes in the ovary, brain, and pituitary were evaluated with the aim of understanding their involvement in the compensatory oocyte development occurring within the remaining ovarian lobe. after 69 days of surgery the remaining ovarian lobe in ulo fish was gravimetrically e ... | 2011 | 21610167 |
| stress-induced effects on feeding behavior and growth performance of the sea bass (dicentrarchus labrax): a self-feeding approach. | repetitive aquaculture-related protocols may act as cyclic stressors that induce chronic stress in cultured fish. the sea bass is particularly sensitive to stressful conditions and the mere presence of humans will disturb feeding behavior. in this paper, we study whether chronic stress induced by repetition of acute stress protocols affects long-term feeding behavior and growth performance in sea bass and whether exogenous cortisol may induce stress-like changes in these parameters. we demonstra ... | 2011 | 21594625 |
| transient receptor potential vanilloid 4 in the european sea bass dicentrarchus labrax: a candidate protein for osmosensing. | the transient receptor potential vanilloid 4 (trpv4) protein is a member of the trp ion channels superfamily that has been proposed as a potential fish osmosensor in previous studies. trpv4 has been widely studied in mammals, particularly for its involvement in sensing the hypotonicity. the european sea bass, dicentrarchus labrax, is a euryhaline teleost that is exposed to salinity changes due to its migrations between the sea and estuaries/lagoons. trpv4 expression and localization in d. labrax ... | 2011 | 21575738 |
| the influence of certain aminoacids and vitamins on post-thaw fish sperm motility, viability and dna fragmentation. | during cryopreservation, dilution in the extender media reduces the seminal plasma constituents being cells more vulnerable to oxidative stress. vitamins (c and e) and the amino acids taurine and hypotaurine are powerful antioxidants naturally present in seminal plasma. whether their effect may improve sperm quality and reduce sperm dna damage after cryopreservation in fish sperm still remains unclear. thus, the aim of the present work was to analyse the effect of extender supplementation with s ... | 2011 | 21482049 |
| molecular cloning and characterization of european seabass (dicentrarchus labrax) and gilthead seabream (sparus aurata) complement component c3. | we present the complete c3 cdna sequence of gilthead seabream (sparus aurata) and european seabass (dicentrarchus labrax) and its molecular characterization with a descriptive analysis of their structural elements. we obtained one sequence for gilthead seabream (gsbc3) which encodes a predicted protein of 1656 amino acids, and two sequences for european seabass (esbc3_1 and esbc3_2) which encode two predicted proteins of 1654 and 1587 amino acids respectively. all sequences present the character ... | 2011 | 21421056 |
| oxytetracycline hydrochloride vital labelling revisited: the case of dicentrarchus labrax and diplodus puntazzo. | the main objective of this study was to determine the optimal oxytetracycline hydrochloride (otc) dosage for otolith marking in dicentrarchus labrax and diplodus puntazzo. adult reared individuals of these two species were marked with otc intraperitoneal injections at three recommended doses (25, 45 and 60 mg kg(-1) fish) and a blank. fishes were monitored for 36-49 days from when they were marked. the effectiveness of the otolith marking was inversely related to the otc dosage. the injections w ... | 2011 | 21366571 |
| analysis of three multigene families as useful tools in species characterization of two closely-related species, dicentrarchus labrax, dicentrarchus punctatus and their hybrids. | by analyzing three multigene families, two closely related and commercially important species, dicentrarchus labrax and dicentrarchus punctatus, were characterized by cytogenetic and molecular methods. the interspecies hybrid dicentrarchus labrax (♀) × dicentrarchus punctatus (♂) was also analyzed. the multigene families studied were the 5s rdna, 45s rdna and the u2 snrna. a microsatellite gtt motif was found within the non transcribed spacers (nts) of the 5s rdna from the two species. however, ... | 2010 | 21317546 |
| the pineal complex of the european sea bass (dicentrarchus labrax): i. histological, immunohistochemical and qpcr study. | the pineal organ of fish is a photosensory and neuroendocrine epithalamic structure that plays a key role in the temporal organisation of physiological and behavioural processes. in this study performed in the european sea bass, dicentrarchus labrax, we provided an in-depth description of the macroscopic and microscopic anatomy of the pineal organ and identified the presence of photoreceptor and presumed melatonin-producing cells using histological and immunohistochemical techniques. in addition ... | 2011 | 21310229 |
| imbalanced dietary ascorbic acid alters molecular pathways involved in skeletogenesis of developing european sea bass (dicentrarchus labrax). | the influence of dietary ascorbic acid (aa) on growth and morphogenesis during the larval development of european sea bass (dicentrarchus labrax) was evaluated until 45days post hatching. diets incorporated 0, 5, 15, 30, 50 or 400mg aa per kg diet to give aa-0, aa-5, aa-15, aa-30, aa-50 and aa-400 dietary treatments, respectively. dietary aa levels lower than 15mg/kg reduced larval growth and survival was affected in specimens fed diets devoid of aa. globally, disruption of the expression of gen ... | 2011 | 21281732 |
| embryonic ionocytes in the european sea bass (dicentrarchus labrax): structure and functionality. | early ionocytes have been studied in the european sea bass (dicentrarchus labrax) embryos. structural and functional aspects were analyzed and compared with those observed in the same conditions (38 ppt) in post hatching stages. immunolocalization of na(+) /k(+) -atpase (nka) in embryos revealed the presence of ionocytes on the yolk sac membrane from a stage 12 pair of somites (s), and an original cluster around the first gill slits from stage 14s. histological investigations suggested that from ... | 2011 | 21261608 |
| antigen-dependent t lymphocytes (tcrβ+) are primarily differentiated in the thymus rather than in other lymphoid tissues in sea bass (dicentrarchus labrax, l.). | all jawed vertebrates share lymphocyte receptors that allow the recognition of pathogens and the discrimination between self and non-self antigens. the t cell transmembrane receptor (tcr) has a central role in the maturation and function of t lymphocytes in vertebrates via an important role in positive selection of the variable region of tcr αβ/γδ chains. in this study, the tcrβ transcript expression and tcrβ(+) cell distribution during the ontogeny of the immune system of sea bass (dicentrarchu ... | 2011 | 21220030 |
| 3d modelling of three pro-inflammatory molecules in selected fish species. | the inflammatory response is the reaction of all metazoan organisms to pathogen invasion that initiates when pathogen-derived molecules are recognized by specific pattern recognition receptors expressed mainly on cells of the innate immune system. the successive expression of pro-inflammatory cytokines and chemokines limits pathogen spread, and attracts and activates immune cells to help in the elimination of the invaders. in this paper we focused on the analyses of the 3d structures of three pr ... | 2010 | 21184658 |
| intestinal t cells of dicentrarchus labrax (l.): gene expression and functional studies. | cellular and molecular data have evidenced a gut-associated lymphoid tissue in a variety of teleost species, abundantly containing t cells, whose origin, selection and functions are still unclear. this study reports cd4, cd8-α, mhci-α, mhcii-β, rag-1 and tcr-β gene transcription along the intestine (anterior, middle and posterior segments) and in the thymus of one year-old dicentrarchus labrax (l.). real-time pcr findings depicted a main role of the thymus in t-cell development, but also rag-1 a ... | 2011 | 21168509 |
| carbonate precipitates and bicarbonate secretion in the intestine of sea bass, dicentrarchus labrax. | the aim of this paper was to study the chemical composition of the precipitates found in the intestine of dicentrarchus labrax and the source of hco(3)(-) secreted into the intestinal lumen. the chemical analysis was performed by employing the potentiometric double titration method and by means of an electron microscope coupled with a spectrometer and x-ray powder diffraction. the results obtained suggest the presence of very insoluble intestinal precipitates, presumably formed by a mixture of c ... | 2011 | 21152925 |
| cloning and sequence analysis of a vasa homolog in the european sea bass (dicentrarchus labrax): tissue distribution and mrna expression levels during early development and sex differentiation. | vasa is a protein expressed mainly in germ cells and conserved across taxa. however, sex-related differences and environmental influences on vasa expression have not been documented. this study characterized the cdna of a vasa homolog in the european sea bass, dicentrarchuslabrax (sb-vasa), a gonochoristic fish with temperature influences on gonadogenesis. the 1911 bp open reading frame predicted a 637-amino acid protein with the eight conserved domains typical of vasa proteins. comparisons of t ... | 2011 | 20955711 |
| transcription of t cell-related genes in teleost fish, and the european sea bass (dicentrarchus labrax) as a model. | in recent years the cloning of genes coding for immuno-regulatory peptides, as well as the sequencing of genomes, provided fish immunologists with a growing amount of information on nucleotide sequences. research is now also addressed in investigating the functional immunology counterpart of nucleotide sequence transcripts in various fish species. in this respect, studies on functional immunology of t cell activities are still at their beginning, and much work is needed to investigate t cell res ... | 2011 | 20950688 |
| application of nonparametric multivariate analyses to the authentication of wild and farmed european sea bass (dicentrarchus labrax). results of a survey on fish sampled in the retail trade. | the aim of this study was to apply biometric measurements and analyses of proximate composition, fatty acid composition, and ratios of stable isotopes of carbon (δ(13)c) and nitrogen (δ(15)n) in muscle tissue to reliably differentiate between wild and farmed european sea bass (dicentrarchus labrax). farmed (n = 20) and wild (n = 19) european sea bass were purchased between march and may 2008 and used as standard samples. in the same months, a survey was conducted to evaluate the truthfulness of ... | 2010 | 20857938 |
| new rt-qpcr assay for viral nervous necrosis virus detection in sea bass, dicentrarchus labrax (l.): application and limits for hatcheries sanitary control. | a sensitive and quantitative one step rt-qpcr method was developed to study viral nervous necrosis (vnn) virus loads in sea bass dicentrarchus labrax (l.) in hatcheries. after determining the limits of this new method, fin tissues were identified as an interesting new simple non-invasive sample source, which might be useful for screening d. labrax (l.) in hatcheries. we observed vnn virus strain v26 associated to d. labrax (l.) eggs and it's release in tank water during spawning suggesting both ... | 2011 | 20837148 |
| golden grey mullet and sea bass oxidative dna damage and clastogenic/aneugenic responses in a contaminated coastal lagoon. | several xenobiotics or their metabolites have redox-cycling properties and potential to induce oxidative stress and dna damage. the current work aimed to study, under environmental conditions, oxidative dna damage (8-hydroxy-2'-deoxyguanosine (8-ohdg)) and its association with chromosomal damage measured as erythrocytic nuclear abnormalities (enas), in liza aurata and dicentrarchus labrax, caught at a costal lagoon (ria de aveiro, portugal) having sites with different contamination profiles. the ... | 2010 | 20825987 |
| risk-taking behaviour variation over time in sea bass dicentrarchus labrax: effects of day-night alternation, fish phenotypic characteristics and selection for growth. | differences in bold and shy personality on sea bass dicentrarchus labrax were investigated between a population (wild) produced from wild-brood fish and a population (selected) produced from selected-brood fish. during the experiment (112 days), fish were reared under self-feeding condition to characterize the feeding behaviour of each individual fish. three risk-taking tests (t1, t2 and t3 of 24 h with day-night alternation) were carried out at >1 month intervals on 180 fish of each strain in o ... | 2009 | 20738645 |
| early development of the digestive tract (pharynx and gut) in the embryos and pre-larvae of the european sea bass dicentrarchus labrax. | the european sea bass dicentrarchus labrax is a marine teleost important in mediterranean aquaculture. the development of the entire digestive tract of d. labrax, including the pharynx, was investigated from early embryonic development to day 5 post hatching (dph), when the mouth opens. the digestive tract is initialized at stage 12 somites independently from two distinct infoldings of the endodermal sheet. in the pharyngeal region, the anterior infolding forms the pharynx and the first gill sli ... | 2009 | 20738616 |
| older triploid fish retain impaired reproductive endocrinology in the european sea bass dicentrarchus labrax. | this paper reports on an evaluation of growth, gonadal development and reproductive endocrinology of older triploid (3n) european sea bass dicentrarchus labrax in comparison with their diploid (2n) counterparts throughout their fifth and seventh annual cycle of life. while older triploids retained impaired reproductive endocrinology, a sexually related dimorphic growth was observed with 3n females attaining the largest sizes. comparisons of some body indexes showed that 3n females had a signific ... | 2009 | 20738514 |
| role of insulin and igf-i on the regulation of glucose metabolism in european sea bass (dicentrarchus labrax) fed with different dietary carbohydrate levels. | the roles of insulin and insulin-like growth factor-i (igf-i) in the regulation of glucose metabolism were assessed in european sea bass juveniles fed with distinct dietary carbohydrate levels. three isonitrogenous diets were formulated to contain 10% (10%pgs) or 30% (30%pgs) pregelatinized starch or no starch (control). the highest plasma glucose and insulin levels were observed 6h after feeding in fish receiving the 30%pgs diet. although plasma igf-i was higher at 6h than at 24h after feeding, ... | 2010 | 20696267 |
| linking behavioural alterations with biomarkers responses in the european seabass dicentrarchus labrax l. exposed to the organophosphate pesticide fenitrothion. | the acute effects of the organophosphate insecticide fenitrothion on dicentrarchus labrax juveniles were investigated through a bioassay using biomarkers and swimming behaviour as effect criteria. after 96 h of exposure to sub-lethal concentrations of fenitrothion, the swimming velocity and several biomarkers were individually determined, namely: brain acetylcholinesterase (ache) activity; muscle cholinesterases (che), lactate dehydrogenase and isocitrate dehydrogenase activities; liver ethoxyre ... | 2010 | 20686920 |
| searching for immunomodulatory sequences in sea bass (dicentrarchus labrax l.): transcripts analysis from thymus. | the thymus is a key organ of the immune system in most vertebrates and, for this reason, it has been used in this paper for the generation of a normalized cdna library from sea bass (dicentrarchus labrax), one of the most extensively cultured species in south mediterranean aquaculture. a total of 1632 ests from this library were initially analysed for sequence quality and vector sequences and, after this control, 1264 (77% of total clones sequenced) high-quality ests were further processed. the ... | 2010 | 20685619 |
| a radiation hybrid map of the european sea bass (dicentrarchus labrax) based on 1581 markers: synteny analysis with model fish genomes. | the selective breeding of fish for aquaculture purposes requires the understanding of the genetic basis of traits such as growth, behaviour, resistance to pathogens and sex determinism. access to well-developed genomic resources is a prerequisite to improve the knowledge of these traits. having this aim in mind, a radiation hybrid (rh) panel of european sea bass (dicentrarchus labrax) was constructed from splenocytes irradiated at 3000 rad, allowing the construction of a 1581 marker rh map. a to ... | 2010 | 20659549 |
| a serum fucose-binding lectin (dlfbl) from adult dicentrarchus labrax is expressed in larva and juvenile tissues and contained in eggs. | the purification, cloning, sequencing, molecular properties and expression of a fucose-binding lectin from the serum of dicentrarchus labrax (dlfbl) have been previously reported. we now describe the distribution and expression of dlfbl during fish ontogeny. immunohistochemistry and in situ hybridization assays were carried out at various developmental stages (from 10 days post-hatching larvae to juveniles). another fucose-binding lectin, similar to dlfbl in biochemical, immunochemical and agglu ... | 2010 | 20596876 |
| immunohistochemical localization of igf-i, igf-ii and mstn proteins during development of triploid sea bass (dicentrarchus labrax). | the cellular localization of igf-i, igf-ii and mstn proteins was investigated during ontogenesis of triploid sea bass (dicentrarchus labrax) by an immunohistochemical approach. the results were compared with those observed in diploids. igf-i immunostaining was mainly observed in skin, skeletal muscle, intestine and gills of both diploids and triploids. from day 30 of larval life, igf-i immunoreactivity observed in skeletal muscle, intestine, gills and kidney was stronger in triploids than in dip ... | 2010 | 20558336 |
| development of an oligo dna microarray for the european sea bass and its application to expression profiling of jaw deformity. | the european sea bass (dicentrarchus labrax) is a marine fish of great importance for fisheries and aquaculture. functional genomics offers the possibility to discover the molecular mechanisms underlying productive traits in farmed fish, and a step towards the application of marker assisted selection methods in this species. to this end, we report here on the development of an oligo dna microarray for d. labrax. | 2010 | 20525278 |