Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| phase-contrast microscopy of cell division in blastocystis hominis. | 1997 | 9307666 | |
| parasitological and nutritional situation of school children in the sukaraja district, west java, indonesia. | a parasitological survey of children aged 8 to 10 years from ten schools located in the rural district sukaraja, west java, indonesia was conducted in december 1995. a total of 348 fecal samples were examined by using modified kato-katz thick smear techniques, 365 blood samples for the measurement of hemoglobin concentration, and anthropometric data were obtained from 404 participants. four nematode (hookworm taken as one species), two cestode and nine protozoan species were detected, but no tre ... | 1997 | 9322303 |
| blastocystis hominis infection in cuban aids patients. | 1997 | 9332594 | |
| parasites elicited cross-reacting antibodies to opisthorchis viverrini. | two batches of crude antigens extracted from adult opisthorchis viverrini worms were compared. one was derived from adult worms harvested from the livers of laboratory infected hamsters and another was obtained from worms sedimented from the faeces of opisthorchiasis patients following treatment with praziquantel. sds-page and coomassie brilliant blue staining revealed that the two preparations had similar protein components of which the predominant ones were the 17-18 kda doublet. the antigens ... | 1997 | 9346276 |
| blastocystis hominis in animals: incidence of four serogroups. | in toto, 520 faecal samples from mammals, birds, reptiles, amphibians, fish, and snails were investigated (see table 1). 91 strains of blastocystis hominis could be isolated by culture. however, only 48 of them were suitable for axenisation. 96 percent of samples belonged to four serogroups detected in humans but two strains, one from a pig and another from a duck, could not be classified, suggesting the existence of one or two further serogroups. while humans showed mainly serogroups i and ii, ... | 1997 | 9361389 |
| usefulness of new dna extraction procedure for pcr technique in species identification of entamoeba isolates. | in this study we have developed a modified clark and diamond (1992, 1993) method using the polymerase chain reaction to amplify amoebic ribosomal rna genes that allow either specific detection of entamoeba histolytica s. str. or amoeba species identification. dna was isolated from cultured protozoa or from stool samples (cysts and/or trophozoites). cultures of xenic or axenic material (also stool samples) were treated with easy genomic dna prep or genomic dna prep plus (a&a biotechnology, poland ... | 1997 | 9374591 |
| [extraintestinal blastocystosis. a review of the literature]. | 1997 | 9380947 | |
| [parasitosis in an adult population with chronic gastrointestinal disorders]. | we worked with 185 middle-class patients above 18 years of age, both sexes, who presented diarrhea and/or chronic gastrointestinal disorders. the faeces were collected serially in formol 10% and processed in the following way: direct microscopy, with and without wet staining, concentration by ritchie's method, 1% safranine technique for a specific investigation of cryptosporidium sp., and faecal sieving macroparasites. twenty eight point six of the studied patients showed at least one enteropara ... | 1997 | 9412130 |
| blastocystis hominis among apparently healthy food handlers in jeddah, saudi arabia. | a prospective study was carried out to investigate the prevalence of blastocystis hominis among random sample of apparently healthy food handlers. a total of 250 non saudi males over 21 years of age were examined. ninety (36%) had pathogenic and non pathogenic intestinal parasites. a total of 143 parasites were detected in their stool specimens. twenty were b. hominis (13.99%) while other parasites were 123 (86.01%). b. hominis was found in 20 positive cases (22.22%) with an overall rate of 8%. ... | 1997 | 9425825 |
| [blastocystis hominis, intestinal pathogen]. | 1997 | 9453768 | |
| [external quality assessment in coproparasitology in havana city province, cuba] | an external quality assessment in coproparasitology was carried out in 77 laboratories from havana city. a questionnaire and ten plastic vials with different intestinal parasites in a small nylon bag, duly sealed, were sent to each laboratory. answers were collected during the 72 hours after delivery. results were analyzed by means of a computer program. the majority of the laboratories (70%) passed the test; the municipalities with the worst scores in the province were lisa, marianao, and haban ... | 1997 | 10886830 |
| description of an improved method for blastocystis hominis culture and axenization. | an improved method for blastocystis hominis culture and axenization was developed in the present study. stool samples were cultured in prereduced boeck-drbohlav nhi modified medium (with several modifications) supplemented with antibiotics (0.4% ampicillin, 0.1% streptomycin, 0.0006% amphotericin b). axenization was performed by the combination of partial purification of b. hominis by ficoll-metrizoic acid gradient and inoculation in fresh medium containing active antibiotics against remaining b ... | 1997 | 9000236 |
| cryptosporidiosis and blastocystosis in renal transplant recipients. | some intestinal parasitic infections are frequently seen in renal transplant recipients. parasites such as cryptosporidium spp. and blastocystis hominis are often asymptomatic or responsible for limited infections in normals, but may cause prolonged and heavy infections with gastrointestinal complaints, mainly diarrhea, in immunocompromised patients. such infections can often not be detected by routine diagnostic procedures, but special concentration and staining methods are needed. we investiga ... | 1997 | 9041537 |
| variation in the cyst morphology of blastocystis hominis. | two types of blastocystis hominis cyst are passed in human feces, one with an outer fibrillar coat and the other without it. the outer fibrillar coat is shed as the cyst matures, but another fibrillar coat forms that is closely attached to the cyst wall and is clearly visible at high magnification in the electron microscope. the internal structure of the two cyst form is similar. | 1997 | 9089732 |
| blastocystis hominis as a potential cause of diarrhea in aids patients: a report of six cases in bahia, brazil. | the role of blastocystis hominis as a human pathogen is controversial, although there is some evidence suggesting that it is the agent implicated in causing diarrhea in immunocompromised patients. we report 6 cases of aids patients presenting with diarrhea with no agents identified in their stools exceptb. hominis. in all cases, treatment was followed by complete recovery from symptoms and clearance of b. hominis from the patients' stools. in 2 cases, relapse of diarrhea was followed by a positi ... | 1997 | 11107245 |
| [intestinal parasitosis: our series from 1992 to 1995]. | from 1992 to 1995 in our department 2236 researches of intestinal parasites (2194 on stool specimens, 34 on scotch tests, 8 on enterotests) were carried out on 1200 patients (703 hiv-ab negative and 497 hiv-ab positive). on the whole 387 samples (17.34%) of 203 subjects (16.92%) were found parasitized; 92 (13.08%) patients of them were hiv-ab negative and 111 (22.33%) were hiv-ab positive. we found more frequently blastocystis hominis and giardia intestinalis among hiv-ab negative subjects and c ... | 1996 | 12858028 |
| [blastocystis hominis infection: a case report]. | blastocytis hominis has long been described as a non-pathogenetic protozoan parasite until recently, when claims have been made that it could be the cause of intestinal disorders. the clinical picture of b. hominis consists of non specific abdominal pain, watery diarrhea, anorexia, vomiting and weight loss. rarely a more invasive form of the disease with rectal bleeding can occur. we describe the case of a ten year-old girl who was admitted to our hospital for diarrhea, abdominal pain and fever. ... | 1996 | 9091775 |
| in vitro response of blastocystis hominis against traditional chinese medicine. | this is the first inn vitro study on the activity of 20 kinds of crude extracts of traditional chinese medicine (tcm) on the intestinal parasite, blastocystis hominis using the criteria of living cell count (lcc) and living cell rate (lcr). lcc and lcr were applied as observation indicators, the former as a fixed-quantity and the latter as a fixed-quality method. lcr calculated percentage rate of living cells using eosin-brilliant cresyl blue staining which could differentiate between living cel ... | 1996 | 9121165 |
| [identification of blastocystis hominis in concentrated fecal samples]. | 1996 | 9044649 | |
| intestinal sarcocystosis in thai laborers. | to determine the prevalence of sarcocystis and other intestinal parasites in thai laborers who were going abroad for work, stool examinations of 362 asymptomatic laborers were studied. the four most frequently parasites found in stool were sarcocystis sp (23.2%), opisthorchis viverini (40.3%), hookworm (21.5%), and strongyloides stercoralis (14.1%). giardia intestinalis (5.2%), entamoeba coli (1.7%), endolimax nana (2.5%), blastocystis hominis (4.1%), echinostoma sp (3.6%), trichuris trichiura ( ... | 1996 | 9031398 |
| human parasite finds taxonomic home. | 1996 | 8602239 | |
| obligate anaerobe or not. | 1996 | 8610009 | |
| prevalence of enteropathogens in stools of rural maasai children under five years of age in the maasailand region of the kenyan rift valley. | stool samples were collected during august 1994 from seventy rural maasai children under the age of five years who were living in the maasailand region of the kenyan rift valley. microbiological analysis was carried out on these samples to identify which intestinal pathogens were present among the infant population of the maasai. of the samples studied 54% were pathogen positive. the most common pathogen isolated was giardia lamblia which was detected in 31% of the samples. other pathogens that ... | 1996 | 8625866 |
| [blastocystis hominis in a zone of northern italy]. | 1996 | 8650434 | |
| blastocystis hominis infection. | 1996 | 8684227 | |
| blastocystosis or zierdt-garavelli disease. | 1996 | 8692759 | |
| dna polymorphism revealed by arbitrary primers polymerase chain reaction among blastocystis strains isolated from humans, a chicken, and a reptile. | dna polymorphisms of different strains of blastocystis isolated from humans, a chicken, and a reptile were examined by an arbitrary primer pcr method. two strains of blastocystis hominis isolated from humans in the usa and japan yielded nearly identical pcr products. however, one strain of b. hominis (isolated from a human in singapore) yielded quite different pcr products. blastocystis sp. isolated from a chicken yielded pcr products similar to those of the former two strains, while blastocysti ... | 1996 | 8720942 |
| the occurrence of blastocystis hominis in hiv-infected patients. | 1996 | 8728052 | |
| observations on the ultrastructure and viability of the cystic stage of blastocystis hominis from human feces. | this report describes the ultrastructure and viability of cysts of blastocystis hominis from feces of infected patients. the cysts were round to ovoid, measured 2-5 microns in size, and contained a condensed cytoplasm that had vacuoles of varying sizes, four nuclei, and as many as six cristate mitochondria. the cell wall was rather electron-lucent. surprisingly, chromatoid-like structures were found in the cytoplasm and nucleus of some of the cysts. these have not previously been reported in bla ... | 1996 | 8738284 |
| colony formation of blastocystis hominis in soft agar. | this is the first description of a method for growing axenized blastocystis hominis as colonies in petri dishes containing soft agar. blastocystis cells cultured in two types of agar appeared to show different colonial morphologies as well as differing colony yields. microscopic examination of the colonies revealed many amoeboid and giant cells. many cells were also shown to possess thin filament-like structures that appeared to stretch across the central vacuole. | 1996 | 8740557 |
| production and characterization of murine monoclonal antibodies to blastocystis hominis. | several hybridomas producing antibodies detected by enzyme-linked immunosorbent assay (elisa) were established by fusions of mouse myeloma p3.x63.ag8.u1 with spleen cells from balb/c mice immunized against an isolate of blastocystis hominis. five strongly positive hybrids (6b6, 1d5, 1e7, 4f7 and 4g11) were cloned and all were found to secrete igm monoclonal antibodies. four mabs (6b6, 1e7, 4f7 and 4g11) reacted in immunoblots with a number of b. hominis antigens (mol. wt ranging from 25,000 to 2 ... | 1996 | 8773525 |
| intestinal parasites in children and adults with and without abdominal discomfort from the kathmandu area of nepal. | the present study on intestinal parasites from the kathmandu area of nepal found that in subjectively healthy children (hc) and adults (ha) the total parasite load was 28.1% and 38.8%, respectively, whereas children (adc) and adults (ada) with abdominal discomfort had a load of 62.7% and 67.8%. the prevalence of nematodes in the 4 groups was significantly higher in those with abdominal discomfort, particularly of hookworm, enterobius, and ascaris. hymenolepis nana was the most common tapeworm, a ... | 1996 | 8783972 |
| phylogenetic position of blastocystis hominis that contains cytochrome-free mitochondria, inferred from the protein phylogeny of elongation factor 1 alpha. | 1996 | 8813670 | |
| identification of surface coat carbohydrates in blastocystis hominis by lectin probes. | the carbohydrate residues of the surface coat of 20 axenic cultures of blastocystis hominis were studied using fitc-labelled lectins (cona, wga, dba, hpa, sba, pna, ueai and lpa). the specific affinity of reactive lectins was determinated by competitive inhibition assay with specific carbohydrates or by enzymatic pre-treatment of cells. all stocks strongly bound cona and hpa; wga, ueai and lpa were partially reactive, and the remaining lectins were nonreactive. inhibition assays showed abolition ... | 1996 | 8818733 |
| axenic culture of reptilian blastocystis isolates in monophasic medium and speciation by karyotypic typing. | the growth of axenic reptilian isolates of blastocystis in iscove's modified dulbecco's medium (imdm) was studied and the morphology of the parasite was examined by phase-contrast microscopy. the chromosomal patterns of these reptilian isolates of blastocystis were examined by pulsed-field gel electrophoresis (pfge) and compared with those of b. hominis and b. lapemi, a sea snake blastocystis. imdm with 10% horse serum supported excellent growth of the reptilian blastocystis isolates. the parasi ... | 1996 | 8825212 |
| the diagnosis of blastocystis hominis cysts in human faeces. | blastocystis hominis cysts in faeces can be concentrated on ficoll column. they are pleomorphic in shape having a mean diameter of 6.65 microns (s.d. = 2.32). they may or may not have a membranous layer surrounding them. the membranous layer tends to come off with pressure or dying of the specimen and corresponds to the fibrillar layer observed under the electron microscope. the ficoll concentration technique is also useful for concentrating other protozoan cysts. | 1996 | 8842989 |
| severe blastocystis hominis in an elderly man. | we describe a unique case of severe blastocystis hominis infection in an elderly man with severe dehydration, marked leukocytosis and hypoalbuminaemia after antibiotic treatment for right pneumonia. the patient recovered after treatment with metronidazole. this case presentation demonstrates the ability of b. hominis to induce severe gastrointestinal manifestations and general deterioration, particularly in light of the controversy surrounding its possible potential pathogenicity. we believe, th ... | 1996 | 8842998 |
| gastrointestinal parasite infections. | 1996 | 8843840 | |
| [protozoan infection (blastocystis hominis) concomitant with pseudomonas sp. peritonitis in continuous ambulatory peritoneal dialysis (capd)]. | case-report of protozoal infection (blastocystis bominis) during pseudomonas peritonitis in male patient with intestinal diverticulosis on continuous ambulatory peritoneal dialysis (capd) treatment for chronic renal failure (crf). microscopic morphology and cultural characteristics are summarized from current literature. photographic images in phase contrast from fresh-observation of faeces and peritoneal fluid are reported. although other protozoa (e.g. acanthamoeba free-living) have already be ... | 1996 | 8848771 |
| blastocystis hominis revisited. | blastocystis hominis is a unicellular organism found commonly in the intestinal tract of humans and many other animals. very little is known of the basic biology of the organism, and controversy surrounds its taxonomy and pathogenicity. there morphological forms (vacuolar, granular, and ameboid) have been recognized, but recent studies have revealed several additional forms (cyst, avacuolar, and multivacuolar). the biochemistry of the organism has not been studied to any extent, and organelles a ... | 1996 | 8894352 |
| clonal growth of blastocystis hominis in soft agar with sodium thioglycollate. | the present report describes a method for establishment of colonies of blastocystis hominis from single cells in soft agar. the percentage of colony-forming efficiency (% cfe = number of colonies grown/number of cells inoculated x 100) for the cultures was greatly improved by the addition of sodium thioglycollate. five human blastocystis isolates chosen for this study showed no apparent variation in colonial morphology. isolated colonies were also successfully grown in liquid medium, providing a ... | 1996 | 8897510 |
| intestinal parasitism in san cayetano, corrientes, argentina. | an epidemiologic study was conducted in san cayetano, a village in the province of corrientes, argentina, to determine the prevalence of intestinal parasitoses in children. eighty-eight households were randomly selected. stool samples were collected from 207 children (72% of the school-age population and 12% of the total village population) over a period of six consecutive days, and were subjected to microscopic examination. of the samples examined, 170 (83%) contained one or more parasites, of ... | 1996 | 8897723 |
| [case report of colitis associated with blastocystis hominis infection]. | 1996 | 8905973 | |
| resistance of blastocystis hominis cysts to metronidazole. | blastocystis hominis cysts concentrated from faeces were resistant to metronidazole up to a concentration of 5 mg/ml. a cysticidal drug is therefore needed for radical treatment, to avoid relapse and to prevent transmission. | 1996 | 8911453 |
| hemorrhagic proctosigmoiditis and blastocystis hominis. | 1996 | 8929001 | |
| resistance of blastocystis hominis cysts to chlorine. | 1996 | 8936976 | |
| freeze-fracture cytochemistry of membrane cholesterol in blastocystis hominis. | membrane cholesterol in blastocystis hominis was detected by freeze-fracture methods using a polyene antibiotic, filipin. since the intramembrane particles (imp) were distributed heterogeneously on both plasma and vacuole membranes, many imp-free areas were observed. even in filipin-treated cells, filipin-cholesterol complexes were not detected in imp-free areas on the plasma membrane, whereas on the central vacuole membrane the complexes were mainly observed in imp-free regions. these results i ... | 1996 | 8982792 |
| [chronic diarrheic syndrome from blastocystis hominis]. | 1996 | 8983372 | |
| hemorrhagic proctosigmoiditis and blastocystis hominis infection. | 1996 | 8534017 | |
| intestinal parasitism--protozoa and helminths--in primates at the barcelona zoo. | the faunistic results regarding intestinal parasitism by protozoa and helminths in 21 primate species (three cebidae, thirteen cercopithecidae, one hylobatidae, one lemuridae, three pongidae) are reported. the primate species were housed in four separate galleries. six faecal samples of each host species were subjected to coprological analysis. fifteen parasite species were detected: 11 protozoa (entamoeba coli, e. chattoni, e. hartmanni, iodamoeba bütschlii, endolimax nana, giardia intestinalis ... | 1996 | 9210027 |
| a comparative morphology of blastocystis hominis cysts with and without the "fibrillar layer". | 1995 | 9139399 | |
| [blastocystis hominis, a potentially pathogenic parasite frequently identified after a travel in tropical regions]. | 1995 | 8539222 | |
| polyparasitism in two rural communities with endemic schistosoma mansoni infection in machakos district, kenya. | formol-ether concentration supplemented by fresh saline smears was used to study intestinal parasites in two communities, miu and kitengei, in machakos district, kenya. these communities differed markedly in schistosome associated morbidity, in spite of similar prevalence and intensities of infection as revealed by kato examination, seven helminth and nine protozoan species were detected among 1011 samples examined. more than 60% of the subjects were infected with more than one parasite: one had ... | 1995 | 8544228 |
| [blastocystis hominis: a common commensal in the colon. study of prevalence in different populations of paris]. | to compare the prevalence of blastocystis hominis in different population categories in paris, including immunodepressed subjects. | 1995 | 8545396 |
| blastocystis hominis in human immunodeficiency virus-related diarrhea. | a wide variety of bacterial, viral, and parasitic pathogens can cause severe diarrhea in patients with advanced human immunodeficiency virus (hiv) infection. conflicting evidence exists as to whether blastocystis hominis should also be included among the infectious agents capable of causing hiv-related diarrhea. | 1995 | 8578192 |
| tubulovesicular elements in blastocystis hominis from the caecum of experimentally-infected rats. | by transmission electron microscopy (tem), tubulovesicular elements were seen in blastocystis hominis obtained from the caecum of experimentally-infected rats. these appeared to arise from the peripheral cytoplasm and were rounded, oval or elongate in sections. it is suggested that these elements form a network for transfer of nutrients to the periphery during the process of encystation. | 1995 | 7797363 |
| occurrence of giardia lamblia in children of municipal day-care centers from botucatu, são paulo state, brazil. | considering that the number of day-care centers for pre-school-age children has expanded rapidly in developing countries, and that these institutions presenting conditions that facilitate the transmission of many enteric agents, a parasitological survey was carried out in three municipal day-cares from botucatu: two in the urban area (one in downtown area and the other one in the city periphery area) and the third in the rural area. three separate stool specimens were collected from 147 children ... | 1995 | 8731262 |
| enzyme-linked immunosorbent assay for detection of serum antibody to blastocystis hominis in symptomatic infections. | an enzyme-linked immunosorbent assay was devised in order to search for antibodies against blastocystis hominis in infected humans. reaction proteins were obtained from washed, axenic b. hominis cells, as sonicate. sonicate was diluted to provide 17 and 34 micrograms of protein per well. dilutions of patients' sera were applied, followed by phosphatase-conjugated goat anti-human serum and phosphatase substrate. color was measured at 405 microns wavelength. immunoglobulin g antibodies to high tit ... | 1995 | 7876972 |
| elucidation of the life cycle of the intestinal protozoan blastocystis hominis. | this paper elucidates the status of the different morphological forms of blastocystis and reports the existence of thin- and thick-walled cysts in b. hominis on the basis of current experimental evidence. it is suggested that the thin-walled cysts are autoinfectious, leading to multiplication of the organism in the intestinal tract. the thick-walled cysts are responsible for external transmission via the faecal-oral route. a life cycle for b. hominis is postulated on the basis of these findings. | 1995 | 7501648 |
| serum antibody detected by fluorescent antibody test in patients with symptomatic blastocystis hominis infection. | 1995 | 7501905 | |
| ultrastructure of blastocystis hominis cysts. | blastocystis hominis cysts were concentrated from human faeces by repeated washing in distilled water and centrifugation on ficoll-paque. this procedure gave a concentrated suspension of cysts without yielding any non-cystic forms. the cysts were examined by both transmission (tem) and scanning electron microscopy (sem). cysts were surrounded by a fibrillar layer that appeared porous in sem. many naked cysts without the fibrillar layer were also visible in sem. they were variable in shape but mo ... | 1995 | 7567903 |
| prevalence of intestinal parasitic infection in five farms in holambra, são paulo, brazil. | a parasitological survey was carried out on 222 inhabitants of five farms in holambra, located 30 km north of campinas, são paulo, brazil, on october 1992. approximately 70% of the inhabitants were found to be infected with at least one species of intestinal parasite. the positive rates of 6 helminths and 7 protozoan species detected are as follows: 5.4% ascaris lumbricoides; 8.6% trichuris trichiura; 19.8% necator americanus; 10.4% strongyloides stercoralis; 1.4% enterobius vermicularis; 0.9% h ... | 1995 | 7569635 |
| blastocystis hominis and traveler's diarrhea. | 1995 | 7578716 | |
| is there any reason to continue treating blastocystis infections? | 1995 | 7578717 | |
| is blastocystis hominis a cause of diarrhea in travelers? a prospective controlled study in nepal. | although the pathogenicity of blastocystis hominis has been extensively debated in the medical literature, controlled studies of the association between b. hominis and diarrhea are lacking. we conducted a case-control study among expatriates and tourists in kathmandu, nepal, in which we compared the prevalence of the organism among patients with diarrhea to that among a control group without diarrhea. b. hominis was detected in 56 (30%) of 189 patients with diarrhea, compared with 40 (36%) of 11 ... | 1995 | 7578767 |
| bacteria, viruses, yeasts and protozoans associated with diarrheal disease in singapore. | labile toxin producing enterotoxic e. coli (etec) were the commonest pathogen isolated from diarrheal stools of hospitalized children (21%) and adults (26%) in singapore. salmonellas ranked a close second in children (19%). other bacterial pathogens were isolated from less than 5% of subjects. blastocystis hominis was detected in 4.3% of diarrheal stools when a simple sedimentation technique was used. cryptosporidium was not detected at all. an analysis of yeast counts in smears of diarrheal and ... | 1995 | 7603751 |
| histochemical detection of carbohydrates of blastocystis hominis. | the carbohydrates of blastocystis hominis were detected by histochemical techniques using light and electron microscopy. b. hominis, fixed with various fixatives, followed by treatment with detergents, were stained with periodic acid-schiff (pas) or alcian blue (ab). intense pas reactions were observed in cells fixed with glutaraldehyde or 1/2 karnovsky fixative. the cells fixed with other fixatives showed weak or no reactions with pas staining. similar results were seen in the case of ab stain. ... | 1995 | 7537145 |
| light and electron microscopic localization of lipids in blastocystis hominis. | localization of the lipid of blastocystis hominis was histochemically demonstrated. b. hominis was fixed with glutaraldehyde, treated with detergents, and stained with sudan black b or nile blue. the central vacuole of b. hominis stained with sudan black b showed many black droplets with great variation in staining intensity. in nile blue staining, the central vacuole showed many pinkish granules or homogeneous blue reactions. these results indicated the presence of neutral lipids and/or acidic ... | 1995 | 7544390 |
| practice guidelines for ordering stool ova and parasite testing in a pediatric population. the alberta children's hospital. | a comprehensive utilization review was done of all stool ova parasite examinations performed at a pediatric hospital during a 3-year period from june 1, 1989 to july 1, 1992. a total of 2,652 stool specimens were surveyed from 1,532 children. forty-one percent (1,081) of the workload was from inpatients, 37% (976) was from emergency room (er) visits/other outpatients, and 22% (233) was from patients attending the gastroenterology (gi) clinic. the prevalence of enteric parasites in hospitalized c ... | 1995 | 7677114 |
| biochemical characterisation of human isolates of blastocystis hominis. | sds-page and iso-enzyme analysis of 11 human isolates of blastocystis hominis revealed at least two variants with different polypeptide patterns and two zymodemes, respectively. this is the first iso-enzyme and the second protein analysis to indicate strain differences in b. hominis. | 1995 | 7707340 |
| [comparison of intestinal parasite infestation indexes among hiv positive and negative populations]. | this study was carried out on two groups of patients treated at the josé de san martín clinical hospital. one group was composed of 82 hiv-seropositive patients with no signs of diarrhea, and another one of 300 patients, not suspected of hiv infection, was considered as "control population". stool samples were collected from each patient and examined for intestinal parasites. these determinations were carried out at the department of clinical biochemistry, in the same hospital. three specimens w ... | 1994 | 7715428 |
| blastocystis hominis infection, a cause of human diarrhea. | blastocystis hominis has long been described as a non pathogenic protozoan parasite until recently when claims have been made that it can result in pathogenic conditions. of the 729 stool samples (614 from survey and 115 from pediatric wards) examined, 18.1% of them were found to be positive for one or more intestinal protozoan cyst. the commonest was giardia intestinalis (8.4%) followed by entamoeba coli (7.1%) and entamoeba histolytica (5.1%) in the normal children without symptoms of diarrhea ... | 1994 | 7777913 |
| [morphology of blastocystis hominis in feces and evaluation of parasitological methods]. | a study of the morphology of blastocystis hominis in stool in ninety-four cases humans is described as central body, ameba and granular form were found as previously described. in addition a "globulose" form as a variation of granular form is first described. the central body form (96.8%) was the most abundant form. three parasitological methods as direct microscopical examination sample with saline solution 0.85%, lugol, sudan iii, stained with quensel, iron hematoxylin and culture are evaluate ... | 1994 | 7557277 |
| [prevalence of infection by intestinal helminths and protozoa in school children from a coastal locality in the province of valdivia, chile]. | during july-august 1989, 219 coprological samples from primary school children from niebla and los molinos localities (39 degrees 52's, 73 degrees 26'w) in the coast of province of valdivia, chile, were analysed. prevalence % (in parentheses) of infection by intestinal protozoa and helminths were the followings: entamoeba histolytica: (18.0), entamoeba coli (34.0), endolimax nana (34.4), iodamoeba buetschlii (7.4), blastocystis hominis (64.3), giardia intestinalis (27.9) chilomastix mesnili (0.8 ... | 1994 | 7654292 |
| morphology of the cyst of blastocystis hominis. | 1994 | 7667736 | |
| a comparison of direct microscopy with culture for the diagnosis of blastocystis hominis. | 1994 | 7667737 | |
| morphological diversity of blastocystis hominis in sodium acetate-acetic acid-formalin-preserved stool samples stained with iron hematoxylin. | the objective of this investigation was to study the morphological characteristics of blastocystis hominis in sodium acetate-acetic acid-formalin-preserved stool samples. routinely processed samples were examined for morphological detail, including size, shape, nuclear detail, and central body characteristics. morphological findings revealing the importance of recognizing b. hominis in the diagnostic laboratory are described. | 1994 | 7510311 |
| differentiation of the various stages of blastocystis hominis by acridine orange staining. | acridine orange staining differentiates the cystic and the central body forms of blastocystis hominis and offers a very convenient and easy method to observe the internal structure of the parasite. acridine orange stains the nuclei and the central body of the rounded vacuolar forms of the parasite bright and dull green, respectively. the colour changes to yellow and then to flaming red-orange when the rounded central body forms of the parasite become cystic. | 1994 | 7521862 |
| a concentration technique for obtaining viable cysts of blastocystis hominis from faeces. | 1994 | 7799511 | |
| a multiple fission-like mode of asexual reproduction in blastocystis hominis. | a non-axenic and an axenic isolate of blastocystis hominis have been induced to form cysts in vitro using an encystation medium. the morphology of the parasite at different time points was observed by scanning electron microscopy. in day-2 cultures the cysts were spherical and had a non-uniform, coarse outer surface around the body. a deep, pore-like opening was seen in some of the parasites. most of the cysts from day-4 and day-6 cultures ruptured, revealing small, uniformly sized spherical bod ... | 1994 | 7809004 |
| isolation of blastocystis hominis from sewage. | 1994 | 7825017 | |
| intestinal colonization of symptomatic and asymptomatic schoolchildren with blastocystis hominis. | a study of single stool specimens was done to determine the prevalence of intestinal parasites among 1,000 primary school children. a questionnaire was completed by each child's parents. specimens were examined by using wet-mount preparation, formaline-ether concentration, and sheather's flotation technique. trichrome and acid-fast stains were done. blastocystis hominis was observed in 203 (20.3%) of the specimens examined, and 175 specimens contained this organism in the absence of other pathog ... | 1994 | 7852590 |
| a study of the karyotypic patterns of blastocystis hominis by pulsed-field gradient electrophoresis. | 1994 | 7855129 | |
| touch cytology. a quick, simple, sensitive screening test in the diagnosis of infections of the gastrointestinal mucosa. | to assess the role of touch cytology (imprint from endoscopic biopsy specimens) in the diagnosis of mucosal infections of the gastrointestinal tract, we reviewed all records and specimens of patients seen during a 30-month period. touch cytology was performed by rolling biopsy specimens on glass slides. after air fixation, a rapid staining method similar to may-grünwald-giemsa was used. the following infections and pathogens were diagnosed (in decreasing order of frequency): helicobacter pylori ... | 1994 | 7979897 |
| [blastocystis hominis as a rare arthritogenic pathogen. a case report]. | blastocysts hominis, a protozoon considered to be a nonpathogen intestinal commensal, is now discussed to be a cause of intestinal infection under certain circumstances, e.g., immunosuppression. there are two published cases of blastocysts infection complicated by arthritis which was classified as "reactive" in one case, "infectious" in the other. we report a third case: a 46-year-old female patient developed a chronic diarrhea and oligoarthritis some days after returning from a trip to senegal. ... | 1994 | 8023590 |
| intestinal parasitism in the united states: update on a continuing problem. | to document patterns of intestinal parasitism in the united states, we analyzed results of 216,275 stool specimens examined by the state diagnostic laboratories in 1987; parasites were found in 20.0%. percentages were highest for protozoans: giardia lamblia (7.2%), entamoeba coli and endolimax nana (4.2% each), blastocystis hominis (2.6%), and entamoeba histolytica (0.9%). the most commonly identified helminths were nematodes: hookworm (1.5%), trichuris trichiura (1.2%), and ascaris lumbricoides ... | 1994 | 8024063 |
| incidence of blastocystis hominis in patients with diarrhoea. | we studied the occurrence of the parasite blastocystis hominis in 1066 stool specimens from patients with diarrhoea, and investigated the relationship between the presence of b. hominis in the faeces and the age of patients. the parasite was recovered from 3.7% samples, but as the sole species of micro-organism in the stool it was recovered from 1% samples. there was no statistically significant difference in the number of b. hominis-positive stools between the younger and the older patients (p ... | 1994 | 8034994 |
| blastocystis hominis infection and intestinal injury. | blastocystis hominis is an enteric protozoan associated with clinical illness. to determine the prevalence of intestinal injury in patients with b. hominis infection, the authors prospectively evaluated 18 patients with b. hominis infection by endoscopy and a test of intestinal permeability. seventeen patients had gastrointestinal symptoms. colonic mucosa appeared normal by lower endoscopy in 12 of 13 patients, and was friable slightly in 1. duodenal mucosa was normal by upper endoscopy in nine ... | 1994 | 8042662 |
| invasive blastocystis hominis infection in a child. | 1994 | 8044274 | |
| [intestinal parasitosis in the 90s: new microorganisms in new patients]. | 1994 | 8059032 | |
| ultrastructural changes during in vitro encystment of blastocystis hominis. | the morphological changes occurring in blastocystis hominis at different time points following in vitro encystment were studied by electron microscopy. the following stages of the parasite were sequentially seen: (a) the amoebic form, which was irregular in shape, with a majority of the organelles being concentrated at the condensed cytoplasmic region; (b) the pre-cystic form, which was rounded and had an electron-dense material forming a homogeneous wall around the central body; and (c) the cys ... | 1994 | 8073021 |
| four serologically different groups within the species blastocystis hominis. | 61 strains of blastocystis hominis were isolated from 340 stool specimens coming from 140 patients with intestinal symptoms and from 200 healthy persons. the overall female to male ratio was 1.9 to 1. classification of the 61 isolates of b. hominis was determined with the aid of the immunodiffusion assay. four serologically different groups could be identified. their frequency ratio in the population studied was 39.5%:39.5%:18%:3%. group 3 was found more often than the other three groups in stoo ... | 1994 | 8167435 |
| lipid biosynthesis by axenic strains of blastocystis hominis. | axenic strains of blastocystis hominis incorporated 32p, added to the medium as orthophosphate, into a number of phospholipids, including sphingomyelin, cardiolipin, phosphatidic acid, the phosphoglycerides of choline, ethanolamine, serine, and inositol and some other minor phospholipids. radioactive palmitate and glycerol provided in the growth medium introduced radiolabel into diacylglycerols, triacylglycerols, and all major phosphoglycerides found in the organism. palmitate is a major fatty a ... | 1994 | 8205379 |
| morphology of blastocystis sp. isolated from circus animals. | blastocystis sp. is reported for the first time from faecal samples collected from a camel, a llama, a highland bull and a lion in a travelling circus. fresh faecal specimens were examined by light and electron microscopy, and vacuolar and cyst forms of similar morphology were present in all three ungulates. these cells were smaller than cultured vacuolar cells of blastocystis hominis isolated from humans and contained only a single vacuole in comparison to the multivacuolar cell found in fresh ... | 1993 | 8225774 |
| urticaria by blastocystis hominis. successful treatment with paromomycin. | urticaria and angioedema are easily recognized disorders, but in at least 70 percent of individuals, chronic episodes of urticaria are of unknown causes. we present 10 cases of chronic urticaria associated parasitation by blastocystis hominis. this parasite has not been previously related with urticaria. both intestinal parasitation as well as urticaria responded successfully to paromomycin sulfate. | 1993 | 8237719 |
| evidence of an epidemic of blastocystis hominis infections in preschool children in northern jordan. | blastocystis hominis is now gaining acceptance as an agent of human intestinal disease. a case-control study of the cause of gastroenteritis in children less than 6 years old was conducted. a total of 500 stool specimens were examined by wet mount preparation, formalin-ether concentration, sheather's sugar flotation technique, and permanent stains when necessary. b. hominis was found in 63 (25%) of 250 stool specimens of the cases examined; 38 (15%) of these specimens contained this parasite alo ... | 1993 | 8253970 |
| amebiasis and "nonpathogenic" intestinal protozoa. | infection with single or multiple species of intestinal protozoa is common in humans and can result in either asymptomatic colonization or symptoms of intestinal disease. entamoeba histolytica serves as a paradigm for invasive colonic protozoal infection. the key to diagnosis and treatment of amebiasis is knowledge of the epidemiologic risk factors and clinical manifestations, a rational approach to diagnosis, and an understanding of the sites of action and uses of anti-amebic drugs. this knowle ... | 1993 | 8254155 |
| intestinal parasitic infections in campalagian district, south sulawesi, indonesia. | intestinal parasitic infections were surveyed in the inhabitants of 3 coastal and 2 inland villages of campalagian district, south sulawesi, indonesia, in july 1992. a total of 398 fecal samples were examined by using kato-katz thick smear, harada-mori culture and agar-plate culture techniques. protozoan cysts were examined by formalin ether concentration technique on 380 fecal samples. soil-transmitted helminth infections were highly prevalent with the overall positive rates as follows: ascaris ... | 1993 | 8266235 |
| axenic culture of blastocystis hominis in iscove's modified dulbecco's medium. | 1993 | 8278347 | |
| diagnosis by faecal culture of dientamoeba fragilis infections in australian patients with diarrhoea. | this paper reports the first survey in australia to use faecal culture to detect dientamoeba fragilis in patients with diarrhoea. of 3 different protozoal culture media evaluated on a case of known infection, modified boeck & drbohlav's medium was the most suitable. the organism could be grown from faeces stored for up to 24 h at room temperature, but for only 10 h at 4 degrees c. culture was then used, in combination with microscopy of smears fixed with polyvinyl alcohol and trichrome-stained, ... | 1993 | 8337717 |