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infectivity of different genotypes of human blastocystis hominis isolates in chickens and rats.most blastocystis hominis isolates from humans are believed to be potentially zoonotic. this is because b. hominis isolates found in a variety of other host species have been found to have identical or relatively similar genotypes to those found in human isolates. however, the transmission of human b. hominis isolates to other animals has not been confirmed experimentally. in this study, the infectivity associated with several unique human blastocystis genotypes (subtypes 2, 3, 4 and 7) was ther ...200717251054
intestinal parasitic infection among children and neonatus admitted to ibn-sina hospital, sirt, libya.a total of 350 stool samples from 196 males and 154 female children and neonatus admitted in ibn-sina hospital, sirt, were examined from june 2001 to may 2002, to determine the prevalence of intestinal parasites. intestinal parasitic infections were identified in 196 (56%) of children and neonates. no intestinal helminthic parasites were detected but 13 intestinal protozoan parasites were detected. the most prevalent protozoan was entamoeba histolytica /e. dispar (36.57%); blastocystis hominis ( ...200717985574
prevalence and clinical features of dientamoeba fragilis infections in patients suspected to have intestinal parasitic infection.the present study was conducted to determine the prevalence and clinical features of dientamoebiasis in patients presumed to be infected with intestinal parasites. a total of 168 patients were examined for d. fragilis using microscopy (after wheatley's trichrome staining) and culture (using modified boeck and drbohlav's medium). d. fragilis trophozoites were detected in 15 samples (8.9%) examined using trichrome staining and in 50 samples (29.8%) by culture method. other enteric parasites were c ...200717985591
intestinal protozoa and helminths among terena indians in the state of mato grosso do sul: high prevalence of blastocystis hominis.a parasitological survey was carried out among terena indians living in the tereré settlement in the municipality of sidrolândia, state of mato grosso do sul, brazil. single samples of feces from 313 indians were processed by means of the spontaneous sedimentation method. in the population studied, 73.5% were infected with at least one intestinal parasite or commensal. protozoa predominated. blastocystis hominis (40.9%), entamoeba coli (33.2%) and entamoeba histolytica/entamoeba dispar (31.6%) w ...200718200414
[evaluation of intestinal parasites in a period of four years in the coprology laboratory of a training hospital].parasitological examination of stool samples of 9867 individuals, submitted from both inpatient and outpatient departments to the microbiology department of an 800-bed training hospital between 1.1.2003 and 31.12.2006, revealed that 582 individuals (5.9%) were infected with intestinal parasites. different diagnostic methods were employed in two different periods, and blastocystis hominis, giardia intestinalis and entamoeba histolytica/dispar were found to be the most common parasites, respective ...200718224623
demographic status and prevalence of intestinal parasitic infections in schoolchildren in izmir, turkey.thirteen primary schools from nine areas were randomly selected in the shantytown and apartment districts in izmir. fecal specimens were evaluated with native-lugol, formalin-ethyl-acetate sedimentation and with trichrome staining for protozoa and helminths and with cellophane tape for enterobius vermicularis. of the study group, 33.4% had one or more parasites. the most common parasite was blastocystis hominis (14.6%) followed by enterobius vermicularis (10.1%) and giardia intestinalis (7.8%). ...200717990581
[dot enzyme-linked immunosorbent assay for detection of serum antibody to blastocystis hominis in humans].serum and stool samples were collected from 322 undergraduate students in medical school. using stool in vitro cultivation as golden standard, 178 cases were found blastocystis hominis positive and 144 were negative. dot-elisa was used to examine the serum samples with a sensitivity of 92.1% (164/178) and specificity of 97.1% (141/144). this revealed that dot-elisa can be used for antibody detection against blastocystis hominis.200718038792
the use of the antifungal agent miconazole as an inhibitor of blastocystis hominis growth in entamoeba histolytica/e. dispar cultures.in regions with high prevalence, blastocystis hominis is frequently found in association with entamoeba histolytica/e. dispar in xenic cultures. its exacerbated growth is often superimposed on the growth of amebas, thus impeding the continuation of the amebas in the culture, within a few generations. the present study reports on the excellent efficacy (100%) of the antifungal agent miconazole in eliminating b. hominis from cultures of e. histolytica/e. dispar, thereby maintaining the integrity o ...200717625701
canine parasitic zoonoses in bangkok temples.fecal samples were collected from 204 humans and 229 dogs from 20 different temples in bangkok, as well as communities in the surrounding temple ground areas. human and dog stool samples were examined for intestinal parasites including giardia using zinc sulfate flotation and microscopy. hookworms were the most common parasite in dogs (58.1%) followed by trichuris (20.5%), isospora (10%), giardia (7.9%), toxocara (7.4%), dipylidium caninum (4.4%) and spirometra (3.1%). blastocystis hominis (5.9% ...200717539273
prevalence of intestinal parasites among primary schoolchildren in derna district, libya.the prevalence of intestinal parasites and some hygienic factors was evaluated in primary schoolchildren in derna district. a total of 1039 stool specimens were examined by direct smear and formaline-ether concentration methods. the results showed that 31% of the children were infected with at least one or two parasites. these parasites were giardia lamblia (12.7%), blastocystis hominis (6.7%), entamoeba histolytica/ entamoeba dispar (6.6%), entamoeba coli (3.2%), e. hartmanni (1.0%), enterobius ...200717580578
[evaluation of parasitology laboratory results of a group of people older than 15 years of age in kutahya].this research has been based on microscopic examination of stool specimens taken from 675 persons older than 15 years of age. the examinations were conducted in parasitology laboratory of the dumlupinar university hospital. laboratory examinations of stools were done with native and formalin-ethyl acetate sedimentation methods. different intestinal parasites were found in 52 (7.7%) of the stool specimens. at least one parasite species was found in 3.11% of women and 4.59% of men. the prevalence ...200717594654
[distribution of intestinal parasites detected in the parasitology laboratory of the ege university medical school hospital, in 2005].the aim of this study was to determine the parasite frequency in 3925 patients during 2005 from january 1- december 31 in the parasitology laboratory of the ege university medicine school. during the laboratory investigation, 3925 fecal specimens and cellophane tapes from the patients were examined. after the microscope examination of 3925 feces samples, it was found that 590 (15.03%) of these samples contained one or more intestinal parasites. blastocystis hominis (4.96%), cyclospora spp. (1.91 ...200717594655
[chronic angioedema and blastocystis hominis infection].the presence of urticaria associated with blastocystic hominis infection has been described in very few studies. to the best of our knowledge, no cases of chronic angioedema associated with blastocystic hominis have been published. the clinical and immunological data of a patient with said association is presented. in the last 5 years, a 21 year old woman suffered episodic spells of angioedema which affected her lips, face and upper limbs accompanied by recurring urticaria. the patient continual ...200717712397
evidence of plasmotomy in blastocystis hominis.blastocystis hominis has been regarded as an enigmatic parasite as many aspects of its basic biology remain uncertain. many reproductive processes have been suggested for the organism; however, to date, only the binary fission has been proven. plasmotomy is one of the modes of reproduction previously suggested to be seen in in vitro cultures. the present study provides trichrome and acridine orange staining evidence for the existence of nucleic acid suggestive of division of nucleus into multinu ...200717701428
pathogenicity of blastocystis hominis, a clinical reevaluation.blastocystis (b.) hominis was considered to be a member of normal intestinal flora in the past, but in recent years it has been accepted as a very controversial pathogenic protozoan. in this study, 52 individuals whose stool examination revealed b. hominis were evaluated for clinical symptoms. metronidazole was administered for 2 weeks to the patients infected with b. hominis. after 2 weeks of treatment they were called for a follow-up stool examination. no other bacteriological and parasitologi ...200717918055
prevalence of blastocystis hominis and strongyloides stercoralis infection in okinawa, japan.this study was conducted to clarify the prevalence of blastocystis hominis and strongyloides stercoralis infection in ryukyu university hospital, okinawa, japan, between january 2004 and november 2006. stool samples collected from 3,292 patients were examined by the direct smear method, formalin-ether sedimentation method, and agar plate culture method. the prevalence rate of b. hominis and s. stercoralis infection was 1.0 and 3.4%, respectively. the prevalence rate of b. hominis infection in pa ...200717717704
the effect of blastocystis hominis on the growth status of children.b. hominis is a protozoan parasite commonly found in the human gastrointestinal tract. the pathogenesis of b. hominis is still controversial, although it is one of the most common parasites found in stool samples.200717179909
morphology and reproductive mode of blastocystis hominis in diarrhea and in vitro.the in vitro culture of blastocystis hominis in roswell park memorial institute (rpmi) 1,640 medium containing 20% calf serum is described. the morphological and reproductive mode studies of b. hominis in symptomatic patients' faeces and in further rpmi 1,640 medium-cultured samples were undertaken by light microscopy using iodine staining and hematoxylin staining. three distinct morphological forms, vacuolar, granular and amoeboid, were distinguished in faeces and in vitro cultures. the cystic ...200717216486
colony growth of philippine isolates of blastocystis hominis in simplified, soft agar medium.the agar-cloning technique of blastocystis hominis has been observed in both solid and semisolid agar using iscove's modified dulbecco's medium. in this study, philippine isolates of b. hominis were grown by pour-plate method in semisolid agar using locke's solution. inoculated plates contained 0.7% agar, 10% horse serum, and 0.1% sodium thioglycollate. plates were incubated at 37 degrees c in a microaerophilic jar for 7-10 days. biconvex disk-shaped colonies were seen abound at the bottom half ...200717235545
blastocystis hominis colitis in a haemophilic patient as a cause of lower gastrointestinal bleeding. 200717286783
waterborne transmission of protozoan parasites: a worldwide review of outbreaks and lessons learnt.at least 325 water-associated outbreaks of parasitic protozoan disease have been reported. north american and european outbreaks accounted for 93% of all reports and nearly two-thirds of outbreaks occurred in north america. over 30% of all outbreaks were documented from europe, with the uk accounting for 24% of outbreaks, worldwide. giardia duodenalis and cryptosporidium parvum account for the majority of outbreaks (132; 40.6% and 165; 50.8%, respectively), entamoeba histolytica and cyclospora c ...200717402277
[is blastocystis hominis an opportunist agent?].despite its high prevalence throughout the world, major issues about blastocystis hominis remain unresolved, including fundamental areas such as taxonomy and pathogenicity. sequences of the ssurrna gene place blastocystis in the stramenophiles. analysis of the elongation factor 1-alpha gene, however, indicates similarity to entamoeba histolytica. there is considerable morphological variability and karyotype diversity, and it appears that more than one species is present in humans and animals. in ...200717471409
[evaluation of parasitological laboratory results of a group of people older than 15 years of age in kutahya].this research has been based on microscopic examination of samples of stool specimens taken from 675 persons older than 15 years of age. the examinations were conducted in the parasitological laboratory of the dumlupinar university hospital. laboratory examinations of stools were done with native and formalin-ethyl acetate sedimentation methods. different intestinal parasites were found in 52 (7.7%) of the stool specimens. at least one parasite species was found in 3.11% of women and 4.59% of me ...200717471410
human antibody recognition of anisakidae and trichinella spp. in greenland.high levels of total ige are observed among children in greenland. to evaluate the extent to which anisakidae and trichinella spp. contribute to the high total ige level, an elisa and a western blot were developed for the detection of igg antibodies to anisakidae, based on excretory/secretory antigens from anisakidae larvae. western blots with anisakidae and trichinella antigens discriminated between anisakidae and trichinella infections, enabling cross-reactivity between the two parasite infect ...200717484764
etiologic agents of diarrhea in solid organ recipients.after transplantation, diarrhea may be caused by infectious agents, drug-specific effects, metabolic conditions, or mechanical complications of surgery. determining the cause helps to determine whether to initiate antimicrobial therapy and the duration of treatment. in this study we aimed to determine the causes of diarrhea in kidney or liver recipients. fifty-two diarrhea episodes among 43 solid organ recipients were evaluated. the cause of diarrhea was detected in 43 patients (82.6%). infectio ...200717511817
[blastocystis hominis infection and diarrhoea]. 200717524325
prevalence of intestinal protozoa in an aborigine community in pahang, malaysia.the objective was to estimate the prevalence of intestinal protozoa among the aborigines and to determine the problems regarding the infection. the study was carried out in january 2006 in pos senderut, pahang, malaysia. samples of faeces were collected from children and adults and these were fixed in pva and trichrome staining was carried out. from the 130 individuals studied, 94 (72.3%) were positive with at least one intestinal protozoa. nine intestinal protozoa namely blastocystis hominis, g ...200717568378
[observations on blastocystis hominis and cyclospora cayetanensis in routine parasitological examinations].we report some observations made from routine parasitological examinations on feces. the methods of faust et al. and of spontaneous sedimentation in water are not enough to identify blastocystis hominis. significant percentage presence of this protozoan was found, especially when staining with iron hematoxylin was performed. cyclospora cayetanensis was found in 0.7% of the cases, which suggests that this parasite should also routinely be investigated by appropriate techniques.200717568902
prevalence of enteric protozoa in human immunodeficiency virus (hiv)-positive and hiv-negative men who have sex with men from sydney, australia.a prospective, comparative study of the prevalence of enteric protozoa was determined among human immunodeficiency virus (hiv)- positive and hiv-negative men who have sex with men (msm) in sydney, australia. a total of 1,868 patients submitted stool specimens; 1,246 were from msm (628 hiv positive and 618 hiv positive) and 622 from non-msm were examined over a 36-month period. a total of 651 (52.2%) stool specimens from msm were positive for protozoa compared with 85 (13%) from non-msm. there wa ...200717360882
[investigation of intestinal parasites in food workers in hospitals in aydin, turkey].food workers are an important risk group for intestinal parasite contamination and dissemination. in the present study food workers, working in food preparation and distribution in the adnan menderes university hospital, aydin state hospital and 82. yil state hospital, were screened for the presence of intestinal parasites. out of 58 food workers 22 were females and 36 were males, and the age of workers ranged from 20 to 56. all workers included in the study answered a questionnaire concerned wi ...200717594656
restriction enzyme digestion analysis of pcr-amplified dna of blastocystis hominis isolates.genomic dna of blastocystis isolates released into 0.1% triton x-100 was suitable for amplification and yielded similar results as the genomic dna extracted with standard kit. the specific b. hominis primers (bh1: gct tat ctg gtt gat cct gcc agt and bh2: tga tcc ttc cgc agg ttc acc tac a) successfully produced the pcr product of about 1,770 bp with all the 7 blastocystis isolates tested. the restriction fragment length polymorphism (rflp) patterns yielded by 13 out of 25 restriction endonuclease ...200718613539
prevalence and clinical features of blastocystis hominis infection among patients in sebha, libya.to determine the prevalence and seasonal variation, and to assess the clinical manifestations and treatment of blastocystosis in libyan patients.200721654943
prevalence of intestinal parasites in hiv-positive/aids patients.the aim of this study was to assess the prevalence of intestinal parasites among hiv-positive/aids patients. a control group comprising 30 apparently healthy hiv-negative individuals was included. of the 60 samples collected from the patients and examined, 34 (56.7%) presented with diarrhoea, while 26 (43.3%) had no reported cases of diarrhoea at the time of study. seventeen (50%) of the parasites detected in the 34 patients (those with history of diarrhoea) were diarrhoea-related causative agen ...200622589594
[a case of infant infected by blastocystis hominis]. 200617366972
blastocystis hominis--an emerging and imitating cause of acute abdomen in children.two children aged 12 and 11 years with a similar history of abdominal pain, nausea, vomiting and fever with abdominal tenderness, and muscle guarding at the right lower quadrant for few days were admitted to our hospital. they subsequently developed diarrhea but without clinical relief. just before the decision of laparotomy, both patients were diagnosed as having blastocystis hominis infection with light microscopic examination of the stools and were treated uneventfully with the appropriate an ...200616863863
blastocystis hominis infection in patient with regular dialysis. 200616868812
[the distribution of patients with intestinal parasites presenting at the parasitology laboratory of the mustafa kemal university medical faculty].in this study, fecal specimens of 3,679 patients presenting at the parasitology laboratory of the medical faculty of the mustafa kemal university between september and may 2005 were examined for intestinal parasites. a total 3,679 stool specimens from 1,260 (34.3%) males and 2,419 (65.7%) females for intestinal parasites using the native-lugol method were examined. intestinal parasites were found in 265 (34.23%) male and 509 (65.76%) females. one or more intestinal parasites were detected in 774 ...200617309033
[comparison of the results of examination of fecal samples from students at six months intervals in the alahaci village primary school in sivas].the aim of this study was to determine the distribution of intestinal parasites in primary school children in the alahaci central village in the sivas province, but owing to high prevalence of parasites in soil, new stool specimens were taken from the same subject group after 6 months. in the first stage of investigation, stool samples from a total of 189 children were taken and examined. it was found that the 110 (58.2%) out of 189 subjects were infected with parasites. the distribution of para ...200617309034
[the distribution of intestinal parasites detected in the dokuz eylul university medical faculty hospital between 2003 and 2004].a retrospective evaluation of the data from 7,712 patients with gastrointestinal complaints who presented at the parasitology laboratory of the dokuz eylul university medical faculty hospital between january 2003 and december 2004 was carried out. fecal samples of all patients were examined using native-lugol and the trichrome staining method after sedimentation by the fecal concentration tube. one or more parasites were detected in 495 (6.41%) of the patients. the distribution of the intestinal ...200617309035
protease activity of blastocystis hominis.parasite-derived proteases are important for the parasite life cycle and the pathogenesis of the disease they produce. proteases of intestinal protozoan parasite blastocystis hominis were studied for the first time with azocasein assays and gelatin sds-page analysis. parasitic lysates were found to have high protease activity and nine protease bands of low (20-33 kda) and high (44-75 kda) molecular weights were reported. proteases were found to be ph-dependent and highest proteolytic activity wa ...200616518611
genetic variability of blastocystis hominis isolates in china.to determine if genetic diversity of blastocystis hominis exists in china, 35 b. hominis isolates obtained from 19 asymptomatic infected individuals and 16 patients with gastrointestinal symptoms were genotyped by pcr using seven pairs of known sequenced-tagged site (sts) primers. out of the 35 isolates, 29 were identified as one of the known genotypes, while five isolates showed two distinct genotypes, and one isolate was an unknown genotype as this was negative with all the sts primers. in thi ...200616688468
[vitro culture of blastocystis hominis in medium dmem].to establish the vitro culture of blastocystis hominis (b. h) in medium dmem for the further research on diagnosis, life cycle and pathogenicity of this intestinal protoza.200617290756
[incidence of intestinal parasites among primary school children in malatya].the aim of this study was to determine the incidence of intestinal parasites among primary school children in the central region of malatya and to educate the children about parasitic diseases. during the study, cellophane tape preparations and stool samples that had been prepared using direct mounting methods were examined. in addition the students were informed about intestinal parasites. parasitic infection was observed in 415 (22.5%) out of 1838 students and the highest rate of 10.6% was tha ...200617106853
epidemiology of blastocystis hominis and other intestinal parasites in a vietnamese female immigrant population in southern taiwan.there has been a rapid increase in cross-border female marriage immigrants in taiwan. in this study, 1,434 vietnamese female marriage immigrants arriving between july 1998 and june 2001 were examined for intestinal parasites. most (77.9%) of these women were 20-29 years of age. the prevalence of intestinal parasite infection was 37.7%, and the trend increased from 1999 to 2001 (statistically significant, p < 0.0001), but decreased among age subgroups (p < 0.0001). among the 20 species of intesti ...200616679297
prevalence of intestinal parasitic infections among school children in the central region of thailand.survey the prevalence of intestinal parasitic infections in 14 primary schools of central thailand.200617205876
[impact of blastocystis hominis infection on ultrastructure of intestinal mucosa in mice].to observe the ultrastructural change ot intestinal mucosa in mice infected with blastocystis hominis, and to study the pathogenic mechanism of b. hominis infection.200617094618
intestinal parasites in dakahlia governorate, with different techniques in diagnosing protozoa.a total of 3180 patients attending mansoura university hospitals' clinics, were subjected to stool examination by direct wet smear, formol-ether concentration, original formol-tween concentration, modified formol-tween concentration, modified sheather's sugar floatation, potassium hydroxide concentration and gomori's trichrome stain, and modified kinyoun's acid-fast stain, and ryan's trichrome blue stain for microsporidia. the intestinal helminthes in a descending order of abundance were: s. man ...200617153711
predominance of amoeboid forms of blastocystis hominis in isolates from symptomatic patients.blastocystis hominis is one of the most common human parasites that inhabit the intestinal tract. conflicting reports continue to exist regarding the existence and the functional role of the amoeboid forms in the life cycle of the parasite. the present study investigates the presence of these forms in 20 isolates obtained from ten symptomatic and asymptomatic patients respectively. a total of 10,000 parasite cells per ml from each isolate were inoculated into three culture tubes each containing ...200616323025
[prevalence of intestinal parasites in children of yukpa ethnia in toromo, zulia state, venezuela].yupkpa community in perijá sierra does not have drinking water or sewage elimination systems. thus it becomes a propitious place for infection with intestinal parasites.200616532165
patients with gastrointestinal complains due to enteric parasites, with reference to entamoeba histolytica/dispar as dected by elisa e. histolytica adhesion in stool.a total of 210 patients with gastrointestinal troubles, of both sex and a mean age of 32 +/- 6.1 years, selected from the outpatient's clinics of al-azhar university hospitals. 115 (54.76%) had dysentery, 95 (45.23%) did not have dysentery, 15 (14%) suffered flatulence, 20 (9.52%) had epi-gastric pain, 19 (9.05%) had vague abdominal pain, 5 vomiting (5.2%) and 10 (4.9%) had fever. two symptoms were in 29 (13.81%) patients and three symptoms in 12 (5.71%). of the 210 patients, 20 (9.9%) had helmi ...200616605100
detection of dientamoeba fragilis and blastocystis hominis using a simple staining method. 200616613140
pcr fingerprinting of blastocystis isolated from symptomatic and asymptomatic human hosts.genomic dna from 16 blastocystis hominis isolates comprising of eight asymptomatic isolates (a1-a8) and eight symptomatic isolates (s1-s8) was amplified by arbitrarily primed polymerase chain reaction (ap-pcr) using 38 arbitrary 10-mer primers. six primers (a10, b5, c20, d1, f6, and f10) generated reproducible dna fingerprints. ap-pcr amplification revealed similar dna fingerprints among all symptomatic isolates (s1-s8) with common bands at 850 bp using primer a10, 920 bp using primer b5, and 1. ...200616628457
amoeboid form of blastocystis hominis - a detailed ultrastructural insight.the amoeboid form of blastocystis hominis has been reported infrequently, and its morphological descriptions have yielded conflicting and confusing reports. in the present study, we used the amoeboid forms seen predominantly in symptomatic patients infected with blastocystis to provide detailed descriptions on the fine surface structure and intracellular morphology. scanning electron microscopy revealed the irregular shape of the amoeboid form, with an intercalated fibrillar structure and a high ...200616816959
the prevalence of parasites in commonly used leafy vegetables in south western, saudi arabia.to determine the degree of contamination caused by parasites in commonly used leafy vegetables in abha, kingdom of saudi arabia (ksa).200616680247
some epidemiological aspects of intestinal parasites in women workers before going abroad.a study was conducted in jakarta on 903 women workers before going abroad through stool examination by ritchie's technical method. of the women workers studied, 640 subjects (70.87%) were found to be infected with intestinal parasites either helminthes, protozoa or combination. out of those infected, 451 (70.47%) subjects were infected with intestinal helminthes, namely ascaris lumbricoides (38.13%), trichuris trichiura (28.13%), a combination of necator americanus and ancylostoma duodenale (13. ...200617041558
infection of blastocystis hominis in primary schoolchildren from nakhon pathom province, thailand.a study was conducted to evaluate the infection status of blastocystis hominis in children from four public schools in phuttamonthon district, nakhon pathom province, thailand during november to december 2004. a total of 814 faecal specimens were used for b. hominis cultivation using jones' medium. mixed infections with other intestinal parasites were also examined by formalin ethyl acetate concentration method. it was found that 13.51% (110 of 814) of the children examined were infected with b. ...200617041560
detection of blastocystis hominis in unpreserved stool specimens by using polymerase chain reaction.blastocystis hominis is a common enteric parasite of worldwide distribution. its pathogenetic potential has not yet been established, although numerous case reports suggest that b. hominis may cause the development of various gastrointestinal symptoms and disorders. the detection of the parasite in stool specimens is conventionally done by microscopy of direct smears, fecal concentrates, or permanently stained smears; however, morphology-based diagnosis is problematic. the aim of this study was ...200617152954
[investigation of intestinal parasites in residents of the kayseri karpuzsekisi basin].in this study, we collected stool and cellophane tape samples from residents in four villages located in the kayseri-karpuzsekisi basin in order to investigate the distribution of intestinal parasites. a total of 240 samples collected from hanyeri (40), sariküklü (20), dokuzpinar (80) and karpuzsekisi (120) were investigated. the parasites detected were as follows: blastocystis hominis, 82 (34.16%); enterobius vermicularis, 37 (15.41%); entamoeba coli 26, (10.83%); entamoeba hartmanni, 10 (4.16% ...200617160846
[incidence of intestinal parasites in municipal sanitary workers in malatya].the incidence of intestinal parasites is closely related to such factors as the socio-economic level of the society, nutritional and hygienic habits, climate, environmental conditions, infrastructure and degree of literacy. in this study, the municipal sanitary workers who are regarded as a high risk group in malatya were examined for intestinal parasites. cellophane slides and fecal samples from 241 workers were examined and intestinal parasites were found in 93 (39.0%). the most common parasit ...200617160847
chronic urticaria due to blastocystis hominis.a 24-year-old woman had a 9-week history of second to third daily urticaria that began after an episode of contact urticaria to topical bufexamac. she was found to have an underlying gastrointestinal infection with blastocystis hominis. this was thought to be clinically relevant as she had a history of mild chronic diarrhoea. after treatment of the blastocystis hominis, her urticaria ceased. this could indicate the importance of performing stool microscopy and culture on all patients with chroni ...200616637808
[blastocystosis or zierdt-garavelli disease: a clinical pathway].the author presents the clinical pathway of blastocystosis, a disease due to blastocystis hominis, an intestinal parasite that has long been enigmatic, which can occasionally be the cause of clinical manifestations.200616913176
[blastocystis hominis and bowel diseases].blastocystis hominis (b. hominis) is a parasite of uncertain role in human disease. it may be identified during a workup for gastrointestinal symptoms, usually in stools. the clinical consequences of b. hominis infection are mainly diarrhea and abdominal pain as well as nonspecific gastrointestinal symptoms such as nausea, anorexia, vomiting, weight loss, lassitude, dizziness, and flatulence. case reports and series have suggested a pathogenic role of b. hominis in causing intestinal inflammatio ...200617106862
comparison of fecal microflora in children with atopic eczema/dermatitis syndrome according to ige sensitization to food.atopic eczema/dermatitis syndrome (aeds) commonly often arises during early infancy. in several intervention studies a beneficial influence on aeds course of certain intestinal bacteria, administered as 'probiotics', has been described. to evaluate the possible role of the natural intestinal microflora in children with allergic eczema/dermatitis syndrome regarding immediate type hypersensitivity to food allergens, children with food allergy (aaeds, n = 68) have been compared with children withou ...200616618364
[enteric parasites and aids in haiti: utility of detection and treatment of intestinal parasites in family members].intestinal parasites and human immunodeficiency virus (hiv) are major health problems in haiti. both entities are known to interact strongly with cell-mediated immunity. the purpose of this study undertaken in port-au-prince, haiti was to evaluate the risk of enteric parasite transmission between hiv-infected patients and family members. routine examination of stool specimens for parasites was conducted in 90 hiv-infected undergoing treatment for intestinal disorders due mainly to cryptosporidiu ...200617201290
[study on the biological characteristic of blastocystis hominis: morphology, mode of reproduction and the relation to bacteria].to observe the reproductive modes of blastocystis hominis and study the relation between this protozoa and bacteria.200617290755
[the prevalence and distribution of intestinal parasites detected by the uludag university medical school].in this study, patients (outpatients and in-patients) with various gastrointestinal system complaints presenting at various clinics of the uludag university medical faculty, bursa, from january 2001-december 2004 were investigated for intestinal parasites. the prevalence of intestinal parasites was evaluated according to age, gender and the months in which cases were seen. saline and iodine preparations from 8,351 stool samples before and after formal-ethyl acetate concentration were examined un ...200617309032
frequency of giardia lamblia among children in dohuk, northern iraq.out of 1261 stool specimens collected from children in dohuk city, northern iraq, the prevalence of giardia lamblia infection was 38.5%. the highest rate of infection was in orphan care centres (48.1%) and the lowest in the paediatric hospital (31.3%). the age group 10-12 years had the highest rate (81.2%) and 7-9 years the lowest (22.9%); boys had a higher rate than girls. some infected samples (70/486) showed double or triple infections and g. lamblia was combined with hymenolepis nana, blasto ...200617333793
[the distribution of intestinal parasites in students of the mustafa kemal university school of health.].intestinal parasites are an important national health problem in our country as in the rest of the world. in our study, the prevalence of intestinal parasites in female students (aged from 16-18 years) in the mustafa kemal university school of health was investigated. fecal samples and cellophane tape preparations were used for diagnosis. for this reason 142 fecal samples and 136 cellophane tape preparations were examined. one or more parasites were found in 65 (45.77%). fecal samples. the preva ...200517124683
[distribution of intestinal parasites in children aged between 0 and 14 presenting at the laboratory of parasitology of the mustafa kemal university medical school.]the aim of this study was to determine the parasite frequency in children from 0-14 years old who had presented at the parasitology laboratory of the mustafa kemal university medicine school complaining of gastrointestinal and anal pruritus. during the laboratory investigation, 561 fecal specimens and 534 cellophane tapes obtained from the patients were examined. after the microscope examination of 561 feces samples, it was found that 104 of these samples (from 50 boys and 54 girls) contained on ...200517124682
[distribution of intestinal parasites among patients who presented at the department of parasitology of the erciyes university medical school.]in this study carried out from 2000-2004, a total of 34,883 stool samples were examined using native-lugol and flotation/sedimentation methods and 9,879 cellophane tape preparations were examined directly. intestinal parasites were found in 9,704 (27.8%) of the specimens. the parasites that were found and their prevalence is as follows: blastocystis hominis, 6,723 (19.3%); entamoeba coli, 1,007 (2.9%); giardia intestinalis, 892 (2.6%); entamoeba histolytica/e. dispar, 798 (2.3%); endolimax nana, ...200517124684
[distribution of intestinal parasites in children from the 23 nisan primary school in hakkari.].this study was performed on children ranging from 6-14 years old coming from 23 nisan primary school in hakkari. in this study, a total of 114 stool samples from 60 male students and 54 female students were examined for intestinal parasites. stool containers were given to the students. after the stool containers were collected, they were taken to the faculty of medicine of the yüzüncü yil university and examined using native lugol and flotation methods. one or more intestinal parasites were foun ...200517124686
[epidemiologic and laboratory assessments of etiologic implications of blastocystis hominis in gastrointestinal diseases].authors present the first laboratory and epidemiological results which reveal the circulation in a population of protozoan blastocystis hominis and its implication in the determinations of some gastrointestinal troubles, with fever, diarrhea and constipation, intense intestinal meteorism, associated with abdominal pain and cramps. out of the 3106 investigated patients, 9.7% presented b. hominis as a unique etiologic agent, with an increased prevalence in adults (74.3%) and women (65.3%). blastoc ...200516607843
the effects of extracts from anti-diarrheic thai medicinal plants on the in vitro growth of the intestinal protozoa parasite: blastocystis hominis.the activities of n-hexane, dichloromethane and methanol extracts from five anti-diarrheic thai medicinal plants, acacia catechu (fabaceae) resin, amaranthus spinosus (amaranthaceae) whole plant, brucea javanica (simaroubaceae) seed, piper longum (piperaceae) fruit and quercus infectoria (fagaceae) nut gall were tested against the in vitro growth of fresh isolates of the intestinal protozoan parasite, blastocystis hominis. the extracts at concentrations that ranged from 62.5 to 2000 microg/ml, w ...200515763365
programmed cell death in blastocystis hominis occurs independently of caspase and mitochondrial pathways.we demonstrated previously that a cytotoxic monoclonal antibody (mab) 1d5 elicits a programmed cell death (pcd) response in blastocystis hominis and showed that caspase-3-like protease influences but is not essential for pcd in mab 1d5-treated b. hominis. we also showed that mitochondrial dysregulation played a role in cell death. in the current study, we further analyzed the signaling pathways involved in pcd mediated by mab 1d5. b. hominis cells were treated with mab 1d5 or control mab 5, eith ...200515913879
[clinical symptoms in cases caused by entamoeba coli and blastocystis hominis.]e. coli and b. hominis are usually accepted as members of normal intestinal flora during stool examinations, but in recent years there has been controversy as to whether they may be pathogen protozoa. in this study, 92 individuals who were found to have e. coli (58/92) and b. hominis (34/92) in their stools were included in a study of clinical symptoms. no other parasitological or bacteriological agents were found in the stools of these persons. the percentages of intestinal symptoms were found ...200517124674
[blastocystosis: a high prevalence of cases found in patients from health center of soledad, anzoategui state, venezuela].the parasitological examination of fecal samples from 98 patients from an ambulatory health center of soledad, anzoategui, venezuela revealed a rather high proportion of results positive for blastocystis hominis (46.9%).200516082488
blastocystis hominis among symptomatic and asymptomatic individuals in talkha center, dakahlia governorate, egypt.blastocystis hominis is now getting acceptance as an agent of human intestinal disease. b. hominis in stool samples of symptomatic and asymptomatic individuals was evaluated as a possible cause of gastro-intestinal troubles. b. hominis was found in 106 (10.1%) out of 1050 individuals examined from six villages and one city in talkha center, dakahlia governorate. the highest infection rate was in manshayt el-badawy village (25.47%), whereas talkha city showed the lowest rate (4.73%). age group 10 ...200516083074
prevalence and effectiveness of an education program on intestinal pathogens in food handlers.to assess the prevalence of intestinal pathogens and the effectiveness of an education program in food handlers in a tertiary care hospital.200516850637
the association of blastocystis hominis and endolimax nana with diarrheal stools in zambian school-age children.to determine the prevalence of endoparasites and their association with diarrhea, a survey was conducted in the southern province of zambia that used conventional and molecular techniques applied to stool and urine samples from school-age children (n = 93). almost half of the stools (49.5%) were diarrhetic. the overall prevalence of endolimax nana, schistosoma haematobium, blastocystis hominis, giardia lamblia, cryptosporidium parvum, encephalitozoon intestinalis, and strongyloides stercoralis w ...200516249910
[prevalence of giardiasis and intestinal parasites in pre-school children from homes being attended as part of a state programme in armenia, colombia].determining the prevalence of giardiasis and intestinal parasitism in pre-school children from the instituto colombiano de bienestar familiar (icbf).200516396421
prevalence of blastocystis hominis infection in asymptomatic individuals from bangkok, thailand.fresh stool examination was performed from 2,230 participants who enrolled in annual check-up programs of the faculty of medical technology, mahidol university in 1999-2000 and 2004. in this study, blastocystis hominis infection was diagnosed by culturing in jones' media. a total of 21% of fecal specimens (in 1999-2000) and 13.7% (in 2004) were positive for b. hominis. the vacuolated form was the predominant form found in culture solution after 48 hours of incubation. the distribution of infecti ...200516438173
current infection rate of giardia lamblia in two provinces of thailand.the aim of this study was to survey for the current rate of giardia lamblia infection in three different districts in two provinces of thailand, surin and samut sakhon, in march 2002, october 2003, and march 2004. two districts are rural areas and another is sub-urban. volunteers of any age from surin province and children aged between 5 to 7 years old from three secondary schools in samut sakhon were enrolled for the stool examination. the method used was stool examination by both simple smear ...200516438174
[the distribution of intestinal parasites in patients presenting at the parasitology laboratory of the cumhuriyet university.]this study is concerned with the distribution of intestinal parasites detected in patients who presented at the routine parasitology laboratory of the cumhuriyet university faculty of medicine from may 2002-november 2004. a total of 5057 stool specimens from 2305 (45.6%) males and 2752 (54.4%) females were examined for intestinal parasites using direct examination and flotation methods. intestinal parasites were found in 231 (4.5%) females and 301(5.9%) males. a total of 1313 cellophane tape spe ...200517160838
[the prevalence of intestinal parasites in four different special daytime nursing homes and day-centers in antakya.].in this study, the prevalence of intestinal parasites in 109 students (68 boys, 41 girls) in the 1-6 age group in four different special daytime nursing home and day-centers (special anakucaği day-time nursing home, special evim day-centre and day time nursing home, special doğa daytime nursing home and special yaren daytime nursing home) in the antakya district of hatay was investigated. for this reason 86 feces and 109 cellophane tape preparations were investigated. concentration of feces and ...200517160839
prevalence of blastocystis hominis among "healthy" food handlers in dammam, saudi arabia.a prospective study was carried out to see the prevalence of b. hominis in food handlers in dammam, eastern province of saudi arabia. a total of 17073 food handlers were examined, 8.50% persons were positive for this organism in their stool. in 2.4% of the food handlers, b. hominis was the only parasite, whereas in 1.9% and 4.2% of the cases, this organism was found in association with pathogenic and non pathogenic parasites respectively. we could find the correlation between the dietary habits ...200516083054
degradation of human secretory immunoglobulin a by blastocystis.microbial immunoglobulin a (iga) proteases cleave human secretory iga, promoting the mucosal adhesion of pathogens. to investigate if the enteric protozoan blastocystis degrades human secretory iga, cell lysate and conditioned medium from two species were exposed to immunoglobulin a. secretory iga was cleaved by both cell lysate and conditioned medium with mainly cysteine proteinase activity in b. hominis b isolate and aspartic proteinase activity in b. ratii wr1 isolate. these findings suggest ...200516151742
[cyclosporosis: an emergent parasitosis. (ii) microbiological diagnosis through a new staining technique].describe a new staining technique for the diagnosis of cyclospora infection through lab tests. a differential microscopic diagnosis between cyclospora and blastocystis hominis is conducted.200516333388
effect of nitazoxanide in persistent diarrhea and enteritis associated with blastocystis hominis.the aim of this study was to evaluate the efficacy of nitazoxanide for the treatment of diarrhea and enteritis associated with blastocystis hominis as the sole identified pathogen in children and adults from the nile delta of egypt.200516234044
no correlation between clinical symptoms and blastocystis hominis in immunocompetent individuals.abstract background and aim: previous reports regarding the clinical significance and pathogenicity of blastocystis hominis have been contradictory. the aim of this study was to examine the association between blastocystis and gastrointestinal symptoms in immunocompetent individuals. methods: we monitored over 2800 healthy people for a period of 15 months, and took stool specimens during both asymptomatic periods and during periods of gastrointestinal symptoms. results: after exclusion of indivi ...200516105126
blastocystis hominis and travelers.b. hominis is a unicellular protozoan commonly identified in stool specimens of travelers who have returned from tropical countries. it has a world-wide distribution, and infection is more common in developing countries compared to industrialized nations. clinical features of illness which have been attributed to blastocystis include nausea, anorexia, abdominal pain, flatulence and acute or chronic diarrhea. the preferred method of diagnosis is a permanently stained smear of an unconcentrated st ...200517292002
[experimental infection of mice with blastocystis hominis].to seek a better pathway and proper number of parasites for blastocystis hominis (b.h) infection in normal and immunocompromised icr mice.200516566218
disparities in parasitic infections, perceived ill health and access to health care among poorer and less poor schoolchildren of rural côte d'ivoire.differences in the state of health between rural and urban populations living in africa have been described, yet only few studies analysed inequities within poor rural communities. we investigated disparities in parasitic infections, perceived ill health and access to formal health services among more than 4000 schoolchildren from 57 primary schools in a rural area of western côte d'ivoire, as measured by their socioeconomic status. in a first step, we carried out a cross-sectional parasitologic ...200515655013
etiology of diarrhea in pediatric outpatient settings.the frequency with which bacteria cause diarrhea evaluated in ambulatory settings is often unknown. we attempted to determine the microbiologic etiology of diarrhea in a private pediatric practice (site a) and a clinic serving largely immigrant children (site b) and to establish guidelines for bacterial culture.200515702043
parasitic zoonoses in papua new guinea.relatively few species of zoonotic parasites have been recorded in humans in papua new guinea. a greater number of potentially zoonotic species, mostly nematodes, occur in animals but are yet to be reported from humans. protozoa is the best represented group of those infecting man, with giardia duodenalis, cryptosporidium parvum, cyclospora cayetanesis, toxoplasma gondii, sarcocystis spp., entamoeba polecki, balantidium coli and, possibly, blastocystis hominis. the only zoonotic helminths infect ...200515831107
molecular characterization of blastocystis isolates in the philippines by riboprinting.extensive genomic polymorphism has been demonstrated among morphologically identical blastocystis isolates. to this end, 32 blastocystis isolates from the philippines (12 from humans, 12 from pigs and 8 from chickens) were analyzed genetically by riboprinting or restriction fragment length polymorphism (rflp) analysis of polymerase chain reaction (pcr) amplified small subunit rdna. three distinct riboprint patterns were observed from the hinfi digestion, while four patterns resulted from the rsa ...200515886995
parasites in nosocomial diarrhoea: are they underestimated?nosocomial diarrhoea, defined as diarrhoea occurring more than 72 h after hospital admission, is reported to account for <1% of endemic nosocomial infections and 17% of epidemic nosocomial infections. the yield of diagnoses from stool cultures in nosocomial diarrhoea is low, and information regarding the role of parasites is limited. we conducted a study to determine the responsible bacterial and parasitological pathogens from nosocomial diarrhoea cases in our 2000-bed tertiary care facility ove ...200515949620
comparison of two methods (microscopy and enzyme-linked immunosorbent assay) for the diagnosis of amebiasis.diagnosis of amebiasis is usually performed on a clinical basis alone in most endemic countries having limited economic resources. this epidemiological study was conducted using modern diagnostic tests for amebiasis in the southeastern region of turkey, an endemic area for amebiasis. the population of this study included patients with symptomatic diarrhea/dysentery attending both yuzuncu yil university, van and harran university, sanliurfa, turkey. a total of 380 stool specimens were collected a ...200515955332
blastocystis hominis and the evaluation of efficacy of metronidazole and trimethoprim/sulfamethoxazole.blastocystis hominis is commonly found in the intestinal tract of humans. although the pathogenicity of this unicellular parasite is controversial, anti-protozoan agents are usually administered to infected individuals. at present, the first choice of chemotherapeutic agent is metronidazole as described in the literature. in this study, we evaluated the effects of metronidazole and trimethoprim/sulfamethoxazole (tmp/smx) on persons infected with b.hominis. a total of 104 subjects infected with b ...200515915364
blastocystis hominis and other intestinal parasites in a community of pitanga city, paraná state, brazil.the objective was to estimate the prevalence of blastocystis hominis, to evaluate the effectiveness of different techniques for its diagnosis as well as to estimate the prevalence of other intestinal parasites in the community of campo verde, a district of pitanga. the work was carried out from august to october 2004. samples of feces from children and adults were collected and submitted to the techniques of direct wet mount, flotation in zinc sulphate solution, tube sedimentation, sedimentation ...200516138204
remission of delayed pressure urticaria after eradication of blastocystis hominis. 200516191864
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