Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| pathogenic potential of blastocystis hominis in laboratory mice. | blastocystis hominis is a ubiquitous enteric protozoan whose pathogenic potential is still controversial. this study was carried out to clarify the pathogenecity of b. hominis infection and to study the proper number of parasites for mice infection. a total of 15 albino mice were orally inoculated with b. hominis and divided according to the inoculums, 10(2), 10(5), and 4 x 10(7) b. hominis forms/100 microl saline, into three groups consisting of five mice each, gi, gii giii, respectively. in ad ... | 2010 | 20499092 |
| blastocystis hominis and dientamoeba fragilis in patients fulfilling irritable bowel syndrome criteria. | studies have suggested a possible role for blastocystis hominis and dientamoeba fragilis in the etiology of irritable bowel syndrome (ibs). we studied the prevalence of b. hominis and d. fragilis in patients with ibs-diarrhea (ibs-d). three hundred and thirty patients were enrolled, 171 (52%) with ibs-d and 159 (48%) were controls, respectively. stool microscopy, culture, and polymerase chain reaction (pcr) for b. hominis and d. fragilis were done. b. hominis was positive by stool microscopy in ... | 2010 | 20532564 |
| [the distribution of intestinal parasites detected in the dokuz eylul university medical faculty hospital between 2005 and 2008.] | a retrospective evaluation of the data from 14,246 patients with gastrointestinal complaints who presented at the parasitology laboratory of the dokuz eylul university medical faculty hospital between january 2005 and december 2008 was carried out. fecal samples of all patients were examined using native-lugol and the trichrome and kinyoun acid-fast staining method after sedimentation in fecal concentration tubes. one or more parasites were detected in 1320 (9.3%) of the patients. the distributi ... | 2010 | 20340084 |
| urinary hyaluronidase activity in rats infected with blastocystis hominis--evidence for invasion? | the fact whether blastocystis hominis can invade has always been in question. apart from a few sporadic studies such as that done on gnotobiotic guinea pigs which showed surface invasion and mucosal inflammation of the host's intestine caused by b. hominis infection, no real documentation of invasion has been proven. studies have shown that hyaluronidase is secreted during the penetration into the host's skin and gut by nematode parasites. hyaluronidase activity in protozoa namely entamoeba hist ... | 2010 | 20358228 |
| intestinal parasitic infections among intellectual disability children in rehabilitation centers of northern iran. | parasitic infection is highly prevalent throughout the developing countries of the world. these infections are the major problem in rehabilitation centers for the mentally retarded. there have been many reports about the prevalence of parasitic infection among different groups of people in iran; however, the epidemiological data in intellectual disability (id) individuals are rare and there are no such data from northern iran. the aim of the present study was to determine the incidence of intest ... | 2010 | 20363588 |
| [isoenzyme patterns of the isolates of blastocystis hominis from guangxi]. | to observe malate dehydrogenase and alkaline phosphatase patterns of ten isolates of blastocystis hominis (bh) from guangxi. | 2010 | 20411749 |
| eosinophilia secondary to strongyloides in rhode island. | 2010 | 20415117 | |
| intestinal protozoa infections among patients with ulcerative colitis: prevalence and impact on clinical disease course. | epidemiological and microbiologic studies suggest that enteropathogenic microorganisms play a substantial role in the clinical initiation and relapses of inflammatory bowel disease. | 2010 | 20145404 |
| solubilized antigen of blastocystis hominis facilitates the growth of human colorectal cancer cells, hct116. | blastocystis hominis is one of the most common intestinal protozoan parasites in humans, and reports have shown that blastocystosis is coupled with intestinal disorders. in the past, researchers have developed an in vitro model using b. hominis culture filtrates to investigate its ability in triggering inflammatory cytokine responses and transcription factors in human colonic epithelial cells. studies have also correlated the inflammation by parasitic infection with cancer. the present study pro ... | 2010 | 20165878 |
| [standardization of blastocystis hominis diagnosis using different staining techniques]. | the present study was carried out from march to may 2008, with the aim of evaluating the effectiveness of different techniques for diagnosing blastocystis hominis in a sample of the population attended at the biomedicine laboratory of feevale university, novo hamburgo, rio grande do sul. | 2010 | 20563502 |
| geographical location and age affects the incidence of parasitic infestations in school children. | environmental factors affect the dissemination and distribution of intestinal parasites in human communities. to comprehend the prevalence of parasitic infestation and to examine whether geographical location and age also influence the prevalence of infection, fecal samples from 195 school children (rural = 95; male = 39; female = 56) (urban = 100; male = 60; female = 40) of five age groups ranging from 5 to 11 years in two different socio-economic zones (rural and urban) were screened for speci ... | 2010 | 20699511 |
| intestinal parasites infection among immunocompromised patients in riyadh, saudi arabia. | this study was conducted to determine the prevalence of intestinal parasites among immunocompromised patients in riyadh, saudi arabia. fecal samples were collected from 136 immunocompromised patients (52 females and 84 males), aged 2 to 69 years. specimens were fixed with 10% formalin for 30 min (2-3 g fecal(-1)) and then concentrated by a formalin-ether sedimentation technique. samples were examined as wet saline mounts and in iodine preparation for detection of protozoan oocysts, cysts, helmin ... | 2010 | 20836300 |
| [blastocystis hominis and nonpathogenic enteric protozoa in patients with pulmonary tuberculosis and those with hiv infection]. | blastocystis hominis and nonpathogenic enteric protozoa were diagnosed in 300 patients with pulmonary tuberculosis mainly of its infiltrative form and 500 with stages ii and iii hiv infection; the patients received antituberculosis therapy (att) and antiretroviral therapy (art), respectively. control groups included 200 tashkent dwellers and 350 patients with various noninfectious diseases of the gastrointestinal tract. triple coproscopy was made. b. hominis was significantly more frequently det ... | 2010 | 20873179 |
| [blastocystis hominis infection in a baby with food allergy]. | the paper describes a rare manifestation of blastocystis hominis infection in a 3-month-old baby with food allergy that appeared as atopic dermatitis and coloproctitis induced by food proteins (cow's milk, hen's egg). the fact that food allergy may be one of the predisposing factors in the development of clinical manifestations of b. hominis infection is discussed. the assumption that the amoeboid form of blastocysts accompanies the clinical manifestation of the infection is confirmed. | 2010 | 20873373 |
| [eosinophilia in an adopted native ethiopian girl]. | 2010 | 19482381 | |
| microscopic diagnosis of sodium acetate-acetic acid-formalin-fixed stool samples for helminths and intestinal protozoa: a comparison among european reference laboratories. | the present study aimed to compare the diagnostic performance of different european reference laboratories in diagnosing helminths and intestinal protozoa, using an ether-concentration method applied to sodium acetate-acetic acid-formalin (saf)-preserved faecal samples. in total, 102 stool specimens were analysed during a cross-sectional parasitological survey in urban farming communities in côte d'ivoire. five saf-preserved faecal samples were prepared from each specimen and forwarded to the pa ... | 2010 | 19456836 |
| frequency of human toxocariasis in a rural population from cajamarca, peru determined by dot-elisa test. | the aim of this study was to estimate the frequency of human toxocariasis in cauday district, cajamarca, peru, using a dot-elisa test. from june to october 2005, a total of 256 adult subjects were studied. blood samples were collected for serology by a dot-elisa test and for hematological examination. parasitological examination was also carried out in stool samples to check cross-reactions in the dot-elisa. the frequency observed was 44.92%, with a significant higher proportion of positivity in ... | 2009 | 19390733 |
| [the role of protozoan parasites in etiology of urticaria]. | in order to investigate a possible link between parasites and chronic urticaria, the prevalence of intestinal protozoans in stool samples of individuals with chronic urticaria (n=55) and healthy controls (n=43) were evaluated with native lugol, formol-ethyl acetate concentration and trichrom dye, modified acid fast. in the case of giardia intestinalis (g. intestinalis) elisa was also used. in the study 29.1% of the patients were found to have protozoan (blastocystis hominis + g.intestinalis) inf ... | 2009 | 19598090 |
| [cryptosporidium sp infections and other intestinal parasites in food handlers from zulia state, venezuela]. | cryptosporidiosis in food handlers from venezuela is unknown, being this an important public health problem in immunosuppressed patients. to determine the prevalence of cryptosporidium sp and other intestinal parasites in food handlers from zulia state, one hundred nineteen fecal samples were evaluated by wet mount, concentrated according to ritchie and modified ziehl-neelsen staining. fourteen (11.8%) were positive for cryptosporidium sp and associated with other protozoosis (p < 0.05), being m ... | 2009 | 19418723 |
| intestinal parasitic infections among inhabitants of karaj city, tehran province, iran in 2006-2008. | karaj is an area with large influx of refugee people in iran. to increase knowledge about parasitic infections, we carried out this research during 2006-2008. we recorded the stool examination results and some of their personal characteristics. a total of 13,915 human stools were examined, and 649 (4.7%) were positive for intestinal parasites. among them, 13 (0.09%) had worm and 636 (4.6%) had protozoan infections. maximum infections belonged to giardia intestinalis, and 534 (3.8%) samples had t ... | 2009 | 19724700 |
| frequency of enteric protozoan parasites among patients with gastrointestinal complaints in medical centers of zahedan, iran. | we investigated the prevalence of intestinal protozoan parasites in patients with gastrointestinal complaints in medical centers in zahedan, iran. a total of 1562 stool samples was examined from july 2004 to january 2006 using microscopy (direct smear, formalin-ether concentration), xenic culture and pcr techniques. four hundred and twenty-seven (27.3%) of the patients were infected with one or more intestinal parasites. giardia lamblia (10.1%), entamoeba coli (10%), e. hartmanni (1.7%), blastoc ... | 2009 | 19084249 |
| enteroparasitosis and their ethnographic relationship to food handlers in a tourist and economic center in paraná, southern brazil. | this study reports on the occurrence of enteroparasites based on data from an ethnographic study of food handlers in the city of cascavel, paraná, brazil. fecal material from 343 food handlers of both sexes, between 14 and 75 years of age, was analyzed using lutz, modified ritchie and ziehl-neelsen techniques. ethnographic relationships were investigated by means of specific questionnaires. positive fecal samples were found for 131 (38.2%) handlers. endolimax nana (67.9%) was the predominant spe ... | 2009 | 19229388 |
| a retrospective analysis of prevalence of gastrointestinal parasites among school children in the palajunoj valley of guatemala. | this study retrospectively analyzed demographic factors that may affect the prevalence of intestinal parasites among guatemalan school children. the findings of the study showed that young age, wet season, female gender, and severe malnutrition all correlated positively with increased rates of infection. clinical visits were performed on 10,586 school children aged 5-15 years over a four-year period (2004-2007) in the palajunoj valley of guatemala, during which 5,705 viable stool samples were sc ... | 2009 | 19248646 |
| comment on: frequency of enteric protozoan parasites among patients with gastrointestinal complaints in medical centers of zahedan, iran. | 2009 | 19307007 | |
| blastocystis hominis infection in long-term care facilities in taiwan: prevalence and associated clinical factors. | blastocystis hominis is probably the most common protozoan found in the human gut worldwide. in taiwan, the prevalence of b. hominis infection is yet to be determined but is expected to be relatively higher among foreign workers. no data is available on the prevalence of b. hominis infection in long-term care facilities in taiwan. this study included 713 subjects (552 residents and 161 care workers) from ten long-term care facilities in taiwan who completed stool microscopic examinations with me ... | 2009 | 19488784 |
| genetic analysis of blastocystis hominis isolated from symptomatic and asymptomatic human hosts in egypt. | extensive genomic polymorphism was demonstrated among morphologically identical b. hominis isolates. a genetic diversity would be a powerful tool for identification or classification of b. hominis subtypes. in this study, 14 egyptian b. hominis isolates were collected, 5 of them were isolated from asymptomatic people whose infections were detected during routine medical check-up and 9 were isolated from patients with gastrointestinal symptoms. restriction fragment length polymorphism (rflp) anal ... | 2009 | 19530613 |
| high prevalence of subtype 4 among isolates of blastocystis hominis from symptomatic patients of a health district of valencia (spain). | in order to know the genetic diversity of blastocystis hominis from a health district of valencia (spain) 51 clinical isolates from symptomatic patients, 31 axenic and 20 monoxenic, were ribotyped by analysing the restriction fragment length polymorphism (rflp) of amplicons obtained by polymerase chain reaction (pcr) of small-subunit of ribosomal dna genes (ssu-rdna). for this purpose, dna was subjected to two independent pcr (rd3-rd5, f1-r1) and to three independent treatments with restrictases ... | 2009 | 19471964 |
| elevated levels of urinary hydrogen peroxide, advanced oxidative protein product (aopp) and malondialdehyde in humans infected with intestinal parasites. | oxidative stress has been implicated as an important pathogenic factor in the pathophysiology of various life-threatening diseases such as cancer, cardiovascular diseases and diabetes. it occurs when the production of free radicals (generated during aerobic metabolism, inflammation, and infections) overcome the antioxidant defences in the body. although previous studies have implied that oxidative stress is present in serum of patients with parasitic infection there have been no studies confirmi ... | 2009 | 19154644 |
| prevalence of dientamoeba fragilis among patients consulting complementary medicine practitioners in the british isles. | a high prevalence of dientamoeba fragilis is reported in faecal samples collected from patients attending complementary medicine practitioners in the british isles. specimens were collected directly after passing into sodium acetate-acetic acid-formalin (saf). during two observation periods in 2002-04 and 2005-07, a d fragilis prevalence of 14.6% (n = 543) and 16.9% (n = 421), respectively, was recorded. these results confirm a surprisingly high prevalence of d fragilis among a selected populati ... | 2009 | 18952691 |
| high prevalence of microsporidium infection in hiv-infected patients. | sixty-four patients infected with human immunodeficiency virus (hiv) participated in a study to determine opportunistic enteric pathogens and compare them with the patients' clinical status. the most frequently found pathogens were microsporidium (81.2%), cryptosporidium parvum (20.3%), candida albicans (12.5%) and blastocystis hominis (10.9%). less frequently found pathogens were giardia intestinalis (6.2%), cyclospora (4.7%), opishorchis viverrini ova (3.1%), strongyloides stercoralis larvae ( ... | 2009 | 19323005 |
| intestinal parasitic infections in hiv/aids patients presenting with diarrhoea in jakarta, indonesia. | we investigated the occurrence of intestinal parasites in indonesian hiv/aids patients with chronic diarrhoea prior to administering antiretroviral therapy. the influence of age, cd4(+) cell count and season on parasite occurrence was also studied. in total, 318 unconcentrated stool samples were analysed using lugol's iodine and modified acid fast staining to detect intestinal coccidia. most samples (94.5%) were from males aged 21-40 years with cd4(+) counts < or = 50 cells/mm(3). parasites were ... | 2009 | 19327806 |
| giardiasis in kindergartens: prevalence study in berlin, germany, 2006. | in germany, an increase of notified giardiasis was observed between 2002 (n = 3,101) and 2007 (n = 3,651) with 62% of cases acquired in germany. the highest incidence was reported in 1- to 5-year-olds (2001-2007, 11.5/100,000 on average) and berlin is one of the most affected states (17.5/100,000). we performed a cross-sectional study in five berlin kindergartens differing in socioeconomic status and migrant proportion in order to estimate the prevalence and investigate routes of transmission am ... | 2009 | 19404678 |
| expression of interferon gamma and proinflammatory cytokines in the cecal mucosa of rats experimentally infected with blastocystis sp. strain rn94-9. | blastocystis hominis is a zoonotic intestinal protozoan parasite whose pathogenic potential is still controversial. the aim of the present study was to clarify the pathogenicity of blastocystis parasites in rats. oral inoculation with 1 x 10(5) cysts of blastocystis sp. strain rn94-9 in rats resulted in chronic infection in the cecum at least until 4 weeks after infection. histological examination revealed neither mucosal sloughing nor inflammatory cell infiltration but showed a slight but signi ... | 2009 | 19255785 |
| epidemiology of blastocystis hominis and other intestinal parasites among the immigrant population in northeastern taiwan by routine physical examination for residence approval. | blastocystis hominis has not been reported as an endemic disease in taiwan, but high prevalence rates have been found among immigrants. due to the increasing number of immigrants in taiwan, b. hominis may become a public health problem in taiwan. this study was performed to determine the prevalence of b. hominis among immigrant populations. | 2009 | 20422136 |
| screening for intestinal parasitic infections among myanmar migrant workers in thai food industry: a high-risk transmission. | the impact of intestinal parasitic infections on public health has been neglected. millions of myanmar natives have migrated to work in thailand. we performed a study of intestinal parasitic infections in myanmar-migrants working in the thai food industry. a total of 338 myanmar migrant workers in a food plant at samut sakhon province, thailand, were recruited for this study. 284 (84%) returned requested stool samples. samples were examined for intestinal parasites by means of simple smear, form ... | 2009 | 18815883 |
| serum leptin concentrations in patients with intestinal parasites. | leptin is a protein hormone controlling food intake and energy expenditure. in all infections including parasitic infections there is loss of appetite and anorexia. the aim of the present study was to clarify the relationship between intestinal parasites and serum leptin concentrations in children and adults. forty patients with intestinal parasites and 34 healthy subjects took part in this study. body weight, height and body mass index (bmi) were measured for all patients and controls. patients ... | 2009 | 19851965 |
| [the effect of trimethoprim-sulfamethoxazole in blastocystis hominis infection]. | the aim of this study was to investigate clinical findings and the effects of trimethoprim-sulfamethoxazole (tmp-smx) in cases of blastocystosis. a total of 37 cases whose stool specimens were sent to the parasitology laboratory from the outpatient clinics of our hospital for various reasons were included in the study. only five or more blastocystis hominis were found during examination with direct wet mount using the 40x objective. the stool specimens were tested for other agents (salmonella sp ... | 2009 | 20101575 |
| [intestinal parasitoses detected in primary schoolchildren in the van province]. | this study was carried out on school children in primary schools in the van province in order to investigate the distribution of intestinal parasitoses. the study was conducted in 7-15 year-old primary schoolchildren during the years from 2003-2005. stool specimens were taken from 2975 school children; of these, 1289 were female and 1686, male. the specimens were examined by native-lugol, flotation and trichrome staining methods in the parasitology laboratory of the health research and training ... | 2009 | 20101580 |
| [intestinal parasites detected in süphan primary schoolchildren in van]. | this study was performed in order to determine the distribution of intestinal parasites among students of süphan primary school in van in 2005. in the study, stool specimens were taken from 395 students (166 girls and 229 boys) between 7-15 years of age. the specimens were examined by native-lugol, flotation and trichrome staining methods in the parasitology laboratory of health research and training hospital, yüzüncü yil university. one or more parasite species were detected in 28.9 % of the ch ... | 2009 | 20101581 |
| [the distribution of intestinal parasites among children in denizli]. | intestinal parasitic disease appears to be an important national health problem in our country. parasitic infection distribution is related to the socioeconomic status and environmental factors. the aim of this study was to determine the distribution of intestinal parasites among children in the 1-15 age group in the denizli province. parasitic infection was observed in 256 (10.2%) out of 2518 children. the highest rate of 31.4% was that of giardia intestinalis. the rates of enterobius vermicula ... | 2009 | 20101582 |
| in vivo encystation of blastocystis hominis. | blastocystis from infected stools of a person who showed chronic symptoms of abdominal discomfort and diarrhea were examined over a 6-month period, using transmission electron microscopy, for the ultrastructural changes from vacuolar to cystic stage. the study confirms the irregular shedding phenomenon of the organism previously reported, and for the first time, records sequential changes in encystation in stools collected over a time period. the study also confirms the existence of a precystic ... | 2009 | 19238443 |
| [community participation in the control of intestinal parasitoses at a rural site in argentina]. | to design, implement, and evaluate a complex plan of actions aimed at reducing parasitoses in a rural town in the province of buenos aires, argentina, with the involvement of the community. | 2009 | 20107700 |
| [intestinal parasitosis in indians of the mapuera community (oriximiná, state of pará, brazil): high prevalence of blastocystis hominis and finding of cryptosporidium sp and cyclospora cayetanensis]. | occurrences of intestinal parasitosis in indians of the mapuera community (oriximiná, state of pará, brazil) were evaluated. within the context of group assessment, this study makes a contribution towards adequate knowledge of this subject, which is significant from a medical-sanitary point of view. parasitological examination of feces from 83 individuals, performed using four different methods, could be considered to have reasonable amplitude for establishing diagnoses. protozoan cysts and helm ... | 2009 | 19684989 |
| identification of blastocystis hominis isolates from asymptomatic and symptomatic patients by pcr. | despite years of study, the pathogenic role of blastocytis hominis is still controversial. genotypic differences between the asymptomatic and symptomatic isolates should assist in determining the pathogenicity of blastocystis. in this study, we genotyped 32 blastocystis isolates obtained from 12 asymptomatic healthy individuals and 20 symptomatic patients pain by polymerase chain reaction using known seven kinds of sequence tagged site primers in this study. when we compared genotype of blastocy ... | 2009 | 19685075 |
| [prevalence and risk factors associated with intestinal parasitism in preschool children from the urban area of calarcá, colombia]. | determining intestinal parasite prevalence and their relationship with social and demographic risk factors, hygiene procedures and sanitation in pre-school children. | 2009 | 19721981 |
| [blastocystis--an enigmatic parasite]. | blastocystis can be isolated from roughly 25% of patients suspected of intestinal parasitosis. at least ten subtypes (sts) have been isolated from humans and animals, and recent data demonstrate that the pathogenicity of the parasite is subtype-dependent. for instance, st1 and st7 are more prevalent among patients with symptoms than healthy individuals, whereas st3 predominates among healthy carriers. the article sums up basic aspects of the parasite and gives an introduction to new data and poi ... | 2009 | 19732521 |
| kinetic analysis of antibody responses to blastocystis hominis in sera and intestinal secretions of orally infected mice. | the kinetics of antibody production against blastocystis hominis, an emerging infectious protozoan parasite which causes intestinal disorder in humans and animals, was studied. sera and intestinal secretions were collected from b. hominis-immunized balb/c mice for 8 weeks. flow cytometry was used to monitor the levels of immunoglobulins a (iga), g (igg), and m (igm) from both types of biological samples. the kinetic profile derived from flow cytometry analysis revealed that igm led the early imm ... | 2009 | 19597843 |
| [weight loss]. | 2009 | 19774510 | |
| study of the dynamics of blastocystis hominis reproduction in vitro. | the dynamics of blastocystis hominis reproduction in vitro in pavlova's and nelson-jones media was studied. the time of generation in these media was 21.5 and 16.7 h, respectively. the duration of the lag phase was 1 day, of log phase 2 days, and of the stationary phase 6 days in both cases. the cell count in the logarithmic phase increased at the expense of the vacuolar forms proliferation. during the stationary phase, the granular forms quantitatively predominated over vacuolar forms, the shar ... | 2009 | 19902108 |
| [parasite intestinal infection and factors associated with coccidian infection in adults at public hospital in lima, peru]. | to evaluate the frequency of intestinal parasites and risk factors associated with coccidian infection in patients cared for at a public hospital in lima-peru. | 2009 | 19915754 |
| effect of exogenous adminstration of antioxidants on blastocystis hominis in vivo. | the effect of exogenous administration of antioxidant (anttox) on the course of b. hominis in experimentally infected mice was studied. b. hominis isolates were obtained from 10 gastrointestinal symptomatic adult patients. three groups of 30 infected mice (3/isolate) were used. gi was untreated infected, gii was treated by antox for 4 weeks after infection diagnosis (treatment strategy), and giii antox treated by for 4 weeks before infection (prophylactic strategy). mild pathological changes wer ... | 2008 | 19143124 |
| protein profile and morphometry of cultured human blastocystis hominis from children with gastroenteritis and healthy ones. | a total of 180 children of age group 5-12 years old in both sexes, of whom 90 were symptomatic and negative for other parasites, rotavirus or pathogenic bacteria. another 90 children were asymptomatic, but with b. hominis in stools. direct smear, formaline-ethyl acetate sedimentation concentration, kinyon carbol-fuchin stain, stool culture, enzyme immunoassay, culturing, morphometric study, gel electrophoresis and experimental infection of mice were done. the results showed that the central body ... | 2008 | 18853619 |
| evaluation of the nitric oxide activity against blastocystis hominis in vitro and in vivo. | the effect of exogenous nitric oxide (no) on growth, viability and ultra-structural of b. hominis was assessed in vitro by sodium nitrite (nano2) in 0.6 mm, 0.8 mm & 1 mm concentrations. the viability of b. hominis was identified using neutral red stain. the role of no as an endogenous oxidant was assessed by identifying its level in cecum tissue, ileum tissue, blood and stool elutes of mice infected with b. hominis symptomatic human isolates using reactive nitrogen assay compared to control. in ... | 2008 | 18853625 |
| [staining improvement for blastocystis hominis specimen]. | schaudinn solution was used to fix the blastocystis hominis specimen and an improved harris hematoxylin staining was applied to stain it. the method shows clearer internal structure of the parasite, simpler and less time-consuming than the traditional iron hematoxylin solution. | 2008 | 19288920 |
| bacterial, archaeal, and eukaryal diversity in the intestines of korean people. | the bacterial, archaeal, and eukaryal diversity in fecal samples from ten koreans were analyzed and compared by using the pcr-fingerprinting method, denaturing gradient gel electrophoresis (dgge). the bacteria all belonged to the firmicutes and bacteroidetes phyla, which were known to be the dominant bacterial species in the human intestine. most of the archaeal sequences belonged to the methane-producing archaea but several halophilic archarea-related sequences were also detected unexpectedly. ... | 2008 | 18974948 |
| [the distribution of intestinal parasites and their causative factors in children]. | intestinal parasitic infection prevalence in children who had been brought to our hospital together with related factors. the study was carried out in 400 children who were chosen at random among those brought to our hospital. all stool samples were examined with nativelugol, formol-ether, kinyon acid-fast, methods. the cellophane tape preparations and hemoglobin concentration measurement were used. intestinal parasites found in 156 of 400 (39%) children were giardia intestinalis (19.8%) enterob ... | 2008 | 19156610 |
| intestinal parasitic infections in srimum suburban area of nakhon ratchasima province, thailand. | a survey on intestinal parasitic infections and some risk factors of infection (social, economic and behavioral) was conducted in suburban area at simum subdistrict, mueang district, nakhon ratchasima province during the period of april 3rd to april 11th, 2007. a total of 214 stool samples, from 85 males and 129 females were examined using simple direct smear, kato's thick smear and modified harada-mori filter paper strip culture technique. the results showed that the prevalence rate of protozoa ... | 2008 | 19287363 |
| frequency of blastocystis hominis and other intestinal parasites in stool samples examined at the parasitology laboratory of the school of pharmaceutical sciences at the são paulo state university, araraquara. | blastocystis homins is a protozoan that causes an intestinal infection known as human blastocystosis. this infection is diagnosed by means of parasitological examination of stools and by permanent staining techniques. the present study was developed to evaluate the frequency of blastocystis hominis infection among inhabitants of the araraquara region, state of são paulo, and to compare different methods for investigating this protozoan in feces samples. evaluations on 503 stool samples were perf ... | 2008 | 19142433 |
| an outbreak of gastroenteritis associated with intestinal parasites. | intestinal protozoa are one of the leading causes of waterborne outbreaks. stool samples of 196 residents from a village of izmir, using the public water supply, were collected during an outbreak of gastroenteritis. patients were asked to fill out a questionnaire reporting on gender, age, gastrointestinal symptoms, whether or not there was a toilet in the house, their hygiene practices, and similar symptoms in the household members. of the patients who had gastrointestinal symptoms (74.5%), diar ... | 2008 | 18985582 |
| [the prevalence of intestinal parasites in children brought to the kars maternal and children's hospital with complaints of gastrointestinal symptoms]. | this study was carried out to determine the prevalence of intestinal parasites in 2-6 year-old children who were brought to kars maternal and children's hospital with complaints of gastrointestinal symptoms during march-june 2007. fecal samples were taken from children and brought to the parasitology laboratory of the faculty of veterinary medicine to be examined for intestinal parasites. fecal samples were examined by centrifugal formalin ether, zinc-sulphate floatation, and modified acid fast ... | 2008 | 18985583 |
| [distribution of intestinal parasites in patients presenting at the parasitology laboratory of the medical school of erciyes university between the years of 2005 and 2008]. | in this study carried out from january 2005-june 2008, a total of 28,911 stool samples were examined using native-lugol and flotation/sedimentation methods and 7,164 cellophane tape preparations were examined directly. intestinal parasites were found in 6,975 (24.13%). of the patients, 52.90% were female and 47.10%, male. blastocystis hominis, 5,701 (19.72%); entamoeba coli, 910 (3.15%); giardia intestinalis, 568 (1.96%); endolimax nana, 363 (1.26%); entamoeba hartmann i, 357 (1.23%); entamoeba ... | 2008 | 18985586 |
| prevalence and factors associated with intestinal parasitic infection among children in an urban slum of karachi. | intestinal parasitic infections are endemic worldwide and have been described as constituting the greatest single worldwide cause of illness and disease. poverty, illiteracy, poor hygiene, lack of access to potable water and hot and humid tropical climate are the factors associated with intestinal parasitic infections. the study aimed to estimate prevalence and identify factors associated with intestinal parasitic infections among 1 to 5 years old children residing in an urban slum of karachi pa ... | 2008 | 18997865 |
| is intestinal parasitic infection still a public health concern among saudi children? | to determine the possible health risk associated with intestinal parasite infections among children with gastroenteritis in jeddah, kingdom of saudi arabia. | 2008 | 18998015 |
| cytologic diagnosis of blastocystis hominis in peritoneal fluid: a case report. | blastocystis hominis is the most common parasite identified in s worldwide. although it is commonly identified in stool preparations, unusual to encounter b hominis in abdominal fluid. | 2008 | 19068678 |
| complete circular dna in the mitochondria-like organelles of blastocystis hominis. | blastocystis hominis is an anaerobic parasite of the human intestinal tract belonging to the stramenopile group. using genome sequencing project data, we describe here the complete sequence of a 29,270-bp circular dna molecule that presents mitochondrial features (such as oxidative phosphorylation complex i subunits) but lacks complexes iii, iv and v. transmission electron microscopy analyses reveal that this molecule, as well as mitochondrial (nadh:ubiquinone oxidoreductase subunit 7 (nad7), be ... | 2008 | 18694756 |
| [the prevalence of microsporidium among patients given a diagnosis of cancer]. | the aim of this study was to determine the frequency of microsporidium among patients given a diagnosis of cancer. for this purpose fecal samples from 320 patients aged 23.60+/-23.00 years were examined using native-lugol and sedimentation methods and evaluated with modified trichrome, trichrome, and calcofluor dyes. moreover a control group of 320 non-cancer patients was set up. while 10.9% of the patient group was found to have microsporidium, only 5.6% of the control group did. comparison bet ... | 2008 | 18645938 |
| high prevalence of blastocystis sp. in pigs reared under intensive growing systems: frequency of ribotypes and associated risk factors. | three hundred and ninety-five pig fecal samples were analyzed looking for blastocystis sp. using optical microscopy and pcr. a global prevalence of 46.8% has been observed in this study, although relative values of prevalence differ notably according to the strata examined, ranging from 9.3% in sows to 75% in weaners. statistic analysis of the data included several risk factors such as different management systems, date of sample collection, fecal consistency, age and sex of the animals. the pre ... | 2008 | 18374492 |
| is blastocystis hominis a new etiologic factor or a coincidence in iron deficiency anemia? | iron deficiency anemia (ida) is a frequent health problem. gut parasites such as n. americanus and a. duodenale are known to cause blood loss, but the role of blastocystis hominis is uncertain. in this study, 212 patients (193 female, 19 male, mean age 41 sd 15 yrs) with ida were enrolled and 90 persons without ida (78 female, 12 male, mean age 45 sd 17 yrs). microscopic examination of stools for b. hominis using the native lugol method was done three times on each subject. if any specimen conta ... | 2008 | 18397391 |
| pathophysiological variability of different genotypes of human blastocystis hominis egyptian isolates in experimentally infected rats. | the genotyping of blastocystis hominis clinical isolates obtained from 28 gastrointestinal symptomatic patients and 16 asymptomatic individuals were identified by polymerase chain reaction using sequenced-tagged site (sts) primers. then, pathophysiological variability between different b. hominis genotypes was evaluated in experimentally infected rats. only four b. hominis subtypes (1, 2, 3, and 4) were detected (18.2%, 9.1%, 54.5%, and 18.2%, respectively) in human isolates. in symptomatic isol ... | 2008 | 18193282 |
| novel algorithm for coexpression detection in time-varying microarray data sets. | when analyzing the results of microarray experiments, biologists generally use unsupervised categorization tools. however, such tools regard each time point as an independent dimension and utilize the euclidean distance to compute the similarities between expressions. furthermore, some of these methods require the number of clusters to be determined in advance, which is clearly impossible in the case of a new dataset. therefore, this study proposes a novel scheme, designated as the variation-bas ... | 2008 | 18245881 |
| giardiasis and other intestinal parasitic infections in relation to anthropometric indicators of malnutrition: a large, population-based survey of schoolchildren in tehran. | the morbidity of intestinal parasitic infections is greatest among children of school age and may have an adverse effect on growth. in the present, cross-sectional study, the association between previously undiagnosed intestinal parasitic infections and growth was assessed in 19,209 children attending elementary schools in tehran. the physical growth of these children was investigated by recording body weights, heights and weight-for-age z scores. faecal samples were collected and each checked f ... | 2008 | 18348775 |
| [the frequency of intestinal parasites in primary school children in urban and rural regions]. | the aim of this study was to determine the prevalence of intestinal parasites in primary school children in sivas and to consider the changes in frequency and variety of intestinal parasites in the light of previous studies carried out since 1985. specimens were examined by direct microscopy and staining. in addition, the relationship of intestinal parasites with physical development, success and socio-economic situation was investigated using surveys. intestinal parasites were found in 191 (26. ... | 2008 | 18351553 |
| genomic diversity of blastocystis hominis from patients in southern iran. | 2008 | 18186983 | |
| [investigation of cryptosporidium sp. in workers of the van municipality slaughterhouse and in slaughtered animals]. | this study was carried out in order to investigate the prevalence of cryptosporidium sp. in slaughtered animals and workers of the van municipality slaughterhouse in van. animals slaughtered at different times and workers who had been working in different departments of the slaughter house were included in the study for three months. a total of 309 fecal specimens from animals including 167 sheep, 56 goats and 86 cattle and 87 fecal specimens from workers were examined for cryptosporidium sp. oo ... | 2008 | 18351543 |
| patterns of infection with intestinal parasites in qatar among food handlers and housemaids from different geographical regions of origin. | intestinal parasitic infections were surveyed among recently arrived immigrant workers in qatar destined for employment in food handling occupations. two overlapping datasets (female workers surveyed in 2005 and 2006, and both sexes in 2006) were analyzed. seven species were detected, 3 nematodes (trichuris trichiura, hookworms and ascaris lumbricoides) and 4 protozoans (entamoeba histolytica/dispar, non-pathogenic entamoebae, blastocystis hominis and giardia lamblia). overall prevalence of infe ... | 2008 | 18457813 |
| extensive multiparasitism in a village of yunnan province, people's republic of china, revealed by a suite of diagnostic methods. | intestinal multiparasitism, the accuracy of different diagnostic techniques, and the influence of sampling effort were studied among 215 individuals in a bulang village, yunnan province, people's republic of china. behavioral, demographic, and socioeconomic data were obtained by questionnaire. multiple stool specimens were examined by the kato-katz, koga agar plate, baermann, and ether-concentration methods. eight helminth and 7 protozoa species were diagnosed. the prevalence of each of the 3 ma ... | 2008 | 18458311 |
| clinical and serological evidence of toxocara infection in school children from morrope district, lambayeque, peru. | the aim of this study was to estimate the frequency of human toxocariosis in a child population from morrope district, lambayeque, peru. from october to december 2005, 182 school children (96 male and 86 female) were studied. blood samples were collected for toxocara elisa-igg test and hematological examination. additionally, stool samples were collected for coproparasitological examination to check cross reactions. we found frequency of positives in 32.4% (59/182) with a significant higher prop ... | 2008 | 18488089 |
| a possible link between subtype 2 and asymptomatic infections of blastocystis hominis. | blastocystis hominis is one of the most common eukaryotic organisms in the intestinal tract of humans, while its pathogenic potential is still controversial. a total of 286 stool samples obtained from adult and pediatric patients with or without gastrointestinal symptoms in two hospitals in manisa, turkey, were cultured to detect b. hominis infection. forty-one and 51 isolates were obtained from the adults and children, respectively, and these isolates were subjected to subtyping by polymerase c ... | 2008 | 18523804 |
| prevalence of intestinal parasites in a population in south of tehran, iran. | the aim of this study was to describe epidemiologic characteristics of intestinal parasites in a population in south of tehran, iran. a retrospective cross-sectional study of patients with suspicious intestinal parasitic infections referred to the zakaria razi laboratory in shahre-ray, southern tehran, iran, was conducted from april 21, 2004 to october 20, 2005. all stool samples were examined and socio-demographic informations were retrieved. of 4,371 referred patients, 466 (239 males and 227 f ... | 2008 | 18604414 |
| the prevalence of intestinal parasites in the province of izmir, turkey. | stool samples of a total of 2,047 people in izmir province were examined by wet mount, formalin ethyl acetate concentration, and trichrome staining methods with an aim to reveal the prevalence of intestinal parasites in izmir together with related personal and environmental risk factors. geographical mapping showing the density and variation of the species of intestinal parasites in izmir was done after all maps were scaled, and the coordinates were determined with geomedia5.0. the prevalence of ... | 2008 | 18604653 |
| amoebic liver abscess or is it? | 2008 | 18408101 | |
| clinical significance of blastocystis hominis: experience from a medical center in northern taiwan. | blastocystis hominis is an intestinal protozoan. the pathogenic role of this organism in human beings is still controversial and has varied among reports from different geographic areas. the purpose of this study was to determine the clinical significance of b. hominis in northern taiwan. | 2008 | 18629417 |
| phenotypic and genotypic characterisation of blastocystis hominis isolates implicates subtype 3 as a subtype with pathogenic potential. | despite frequent reports on the presence of blastocystis hominis in human intestinal tract, its pathogenicity remains a matter of intense debate. these discrepancies may be due to the varying pathogenic potential or virulence of the isolates studied. the present study represents the first to investigate both phenotypic and genotypic characteristics of b. hominis obtained from symptomatic and asymptomatic individuals. symptomatic isolates had a significantly greater size range and lower growth ra ... | 2008 | 18795333 |
| [the prevalence of intestinal parasites according to the distribution of the patients' gender and parasite species for five years at the osmangazi university medical faculty]. | in this study, patients (outpatients and inpatients) with various gastrointestinal system complaints presenting at various clinics of the eskisehir osmangazi university medical faculty, from february 2003-december 2007 were investigated for the presence of intestinal parasites. the prevalence of intestinal parasites was evaluated according to parasite species, gender of the patients and the years, in which cases were seen. a total of 34,733 stool samples were prepared by formal-ethyl acetate con ... | 2008 | 18645941 |
| human antibody recognition of anisakidae and trichinella spp. in greenland. | high levels of total ige are observed among children in greenland. to evaluate the extent to which anisakidae and trichinella spp. contribute to the high total ige level, an elisa and a western blot were developed for the detection of igg antibodies to anisakidae, based on excretory/secretory antigens from anisakidae larvae. western blots with anisakidae and trichinella antigens discriminated between anisakidae and trichinella infections, enabling cross-reactivity between the two parasite infect ... | 2007 | 17484764 |
| canine parasitic zoonoses in bangkok temples. | fecal samples were collected from 204 humans and 229 dogs from 20 different temples in bangkok, as well as communities in the surrounding temple ground areas. human and dog stool samples were examined for intestinal parasites including giardia using zinc sulfate flotation and microscopy. hookworms were the most common parasite in dogs (58.1%) followed by trichuris (20.5%), isospora (10%), giardia (7.9%), toxocara (7.4%), dipylidium caninum (4.4%) and spirometra (3.1%). blastocystis hominis (5.9% ... | 2007 | 17539273 |
| infectivity of different genotypes of human blastocystis hominis isolates in chickens and rats. | most blastocystis hominis isolates from humans are believed to be potentially zoonotic. this is because b. hominis isolates found in a variety of other host species have been found to have identical or relatively similar genotypes to those found in human isolates. however, the transmission of human b. hominis isolates to other animals has not been confirmed experimentally. in this study, the infectivity associated with several unique human blastocystis genotypes (subtypes 2, 3, 4 and 7) was ther ... | 2007 | 17251054 |
| [survey of intestinal parasites among an aboriginal community in salta]. | the prevalence of intestinal parasitoses by protozoans and helminths was determined in an aboriginal community located 6 km from tartagal, province of salta, argentina. the age of the inhabitants studied ranged from 1 to 49 years old. a total of 112 stool samples were collected in sodium acetate-acetic acid- formalin solution (saf). each sample was obtained in three different days, alternatively. ritchie biphasic concentration method and faust flotation technique were applied. for survey of ente ... | 2007 | 18422062 |
| morphology and reproductive mode of blastocystis hominis in diarrhea and in vitro. | the in vitro culture of blastocystis hominis in roswell park memorial institute (rpmi) 1,640 medium containing 20% calf serum is described. the morphological and reproductive mode studies of b. hominis in symptomatic patients' faeces and in further rpmi 1,640 medium-cultured samples were undertaken by light microscopy using iodine staining and hematoxylin staining. three distinct morphological forms, vacuolar, granular and amoeboid, were distinguished in faeces and in vitro cultures. the cystic ... | 2007 | 17216486 |
| evaluation of the in vitro effect of nigella sativa aqueous extract on blastocystis hominis isolates. | the effect of nigella sativa aqueous extract was evaluated against the in vitro growth of 2 different isolates of the intestinal protozoan parasite blastocystis hominis. different concentrations (10, 100, 500 microg/ml) of nigella aqueous extract and metronidazole, an active standard drug for b. hominis, were incubated with b. hominis isolates in culture media at 37 degrees c. their possible effect on b. hominnis living cell count (lcc) was assessed on day 1, 3 & 6. the aqueous extract of n. sat ... | 2007 | 18383782 |
| intestinal protozoan and helminth infections in the municipality san juan y martínez, pinar del río, cuba. | a cross-sectional study was carried out to determine the prevalence of intestinal parasitic infections and the intensity of soil-transmitted helminths (sths) among children who attend the educational centres of san juan y martinez, cuba. ascaris lumbricoides, blastocystis hominis and giardia lamblia were the most common parasites found, with prevalence rates of 40.5%, 38.5% and 25%, respectively. in total, 91% of children harboured at least one type of intestinal parasite. the rates and intensit ... | 2007 | 17988492 |
| [blastocystis hominis and diarrhea]. | 2007 | 17988622 | |
| intestinal protozoa and helminths among terena indians in the state of mato grosso do sul: high prevalence of blastocystis hominis. | a parasitological survey was carried out among terena indians living in the tereré settlement in the municipality of sidrolândia, state of mato grosso do sul, brazil. single samples of feces from 313 indians were processed by means of the spontaneous sedimentation method. in the population studied, 73.5% were infected with at least one intestinal parasite or commensal. protozoa predominated. blastocystis hominis (40.9%), entamoeba coli (33.2%) and entamoeba histolytica/entamoeba dispar (31.6%) w ... | 2007 | 18200414 |
| intestinal parasitic infections among school children in thailand. | a study was conducted to determine the prevalence of intestinal parasites in children from eight schools located in phuttamonthon district, nakhon prathom province during november 2004 to december 2004. stool samples were collected from 1920 students; age range from 7 to 12 years old, and examined for intestinal parasites by using formalin-ethyl acetate concentration technique. of these subjects, 242 (12.6%) were infected with one or more of 10 intestinal parasitic species. in these infected sub ... | 2007 | 18209713 |
| characteristics of blastocystis hominis infection in a turkish university hospital. | in order to determine characteristics of blastocystis (b.) hominis infection; 770 individuals' stool specimens were examined both by simple and concentration techniques and stained with iodine solution and trichrome in the parasitology laboratory of hacettepe university faculty of medicine, turkey. among the examined 770 specimens, b. hominis was detected in 94 (12.2%). b. hominis was the most common intestinal parasite among the study group. it was mostly detected with dientamoeba fragilis. amo ... | 2007 | 18224616 |
| [evaluation of intestinal parasites in a period of four years in the coprology laboratory of a training hospital]. | parasitological examination of stool samples of 9867 individuals, submitted from both inpatient and outpatient departments to the microbiology department of an 800-bed training hospital between 1.1.2003 and 31.12.2006, revealed that 582 individuals (5.9%) were infected with intestinal parasites. different diagnostic methods were employed in two different periods, and blastocystis hominis, giardia intestinalis and entamoeba histolytica/dispar were found to be the most common parasites, respective ... | 2007 | 18224623 |
| [prevalence of intestinal parasites among workers in food sector in van region]. | stool and cellophane tape specimens were taken for parasitological examination from 739 people who work in food sector and applied to the public health lab of the van health administration for porter examination. parasites were determined at 131 people (17.71%) of 739 worker whom samples were investigated. ninety-five people had one, 30 people had two, 5 people had three and one person had four parasite species. parasites determined in the study were 19.08% helminthes and 80.91% protozoon. in th ... | 2007 | 18224624 |
| [dot enzyme-linked immunosorbent assay for detection of serum antibody to blastocystis hominis in humans]. | serum and stool samples were collected from 322 undergraduate students in medical school. using stool in vitro cultivation as golden standard, 178 cases were found blastocystis hominis positive and 144 were negative. dot-elisa was used to examine the serum samples with a sensitivity of 92.1% (164/178) and specificity of 97.1% (141/144). this revealed that dot-elisa can be used for antibody detection against blastocystis hominis. | 2007 | 18038792 |
| health inequities: lower socio-economic conditions and higher incidences of intestinal parasites. | intestinal parasitic infections affect child health and development and slow down growth, while reducing adults' productivity and work capacity. the aim of the present study was to determine and compare the incidences of intestinal parasitic infections and the socio-economic status of two near primary school children in manisa, a western city of turkey. | 2007 | 18042287 |
| restriction enzyme digestion analysis of pcr-amplified dna of blastocystis hominis isolates. | genomic dna of blastocystis isolates released into 0.1% triton x-100 was suitable for amplification and yielded similar results as the genomic dna extracted with standard kit. the specific b. hominis primers (bh1: gct tat ctg gtt gat cct gcc agt and bh2: tga tcc ttc cgc agg ttc acc tac a) successfully produced the pcr product of about 1,770 bp with all the 7 blastocystis isolates tested. the restriction fragment length polymorphism (rflp) patterns yielded by 13 out of 25 restriction endonuclease ... | 2007 | 18613539 |