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a cross-sectional study on intestinal parasitic infections in rural communities, northeast thailand.despite the existence of effective anthelmintics, parasitic infections remain a major public health problem in southeast asia, including thailand. in rural communities, continuing infection is often reinforced by dietary habits that have a strong cultural basis and by poor personal hygiene and sanitation. this study presents a survey of the prevalence of intestinal parasitic infections among the people in rural thailand. the community-based cross-sectional study was conducted in villages in khon ...201324516280
[the prevalence of intestinal parasites in children with helicobacter pylori gastritis evaluated retrospectively].h. pylori infection is more frequent and is seen in younger ages in developing countries when compared to developed countries. etiopathogenetic factors include living in crowded families, low educational level of mother, low income and infected drinking water. intestinal parasites are more frequent in low socioeconomical populations. in this study, it was aimed to determine the prevalence of intestinal parasite in patients with h. pylori gastritis proven with endoscopic and histopathological stu ...201324412863
blastocystis: consensus of treatment and controversies.blastocystis is a highly controversial protozoan parasite. it has been variably regarded as a commensal and pathogen. scientists have for decades wondered whether it is truly an enteropathogen and if it is observed in symptomatic patients whether treatment is required because patient recovery and improvement has been noted even without any treatment. though associated with self-limiting infection, treatment is warranted in many patients due to persistence of symptoms. this particularly holds tru ...201323961439
[the case of henoch-schönlein purpura associated with blastocystis hominis].blastocystis hominis (b. hominis) is a parasite that often causes gastrointestinal symptoms in patients with immune deficiency and has a controversial pathogenicity in healthy people, although some symptoms are reported outside of the gastrointestinal system in healthy persons. henoch-schönlein purpura (hsp) vasculitis is an acute autoimmune disease characterised by iga storage of small vessels that is believed to include infectious factors in its aetiology. a 30-month follow-up with a boy diagn ...201323955912
[relationship between morphology and pathogenicity of blastocystis hominis trophozoites].six hundred and eighty-six fresh fecal specimens were collected from outpatients (663 well-formed feces and 23 watery feces) during march 2011 to march 2012. all specimens were examined microscopically by direct smear and iodine stained method. b. hominis obtained from the human positive fecal specimens were cultured in les medium, and inoculated into the abdominal cavity of 10 female mice of 6-8-week old. the abdominal fluid was examined with same methods. 103 of 686 patients were positive (80 ...201324809197
hiv and intestinal parasite co-infections among a chinese population: an immunological profile.parasite infections often result in a switch of the human body's predominant immune reaction from t-helper 1 (th1)-type to th2-type. hence, parasite infections are widely expected to accelerate the progression of human immunodeficiency virus (hiv) infections to acquired immunodeficiency syndrome (aids). in the people's republic of china, both parasitic diseases and aids are epidemic in certain rural areas, and co-infections are relatively common. however, no population-based studies have yet inv ...201323971713
nutritional status in parasitized and nonparasitized children from two districts of buenos aires, argentina.the program for the control of intestinal parasites and nutrition was designed to intervene in small communities to prevent and control the effects of parasitic infections on children's health.201324243498
hypoalbuminemia as a predictor of diarrhea caused by blastocystis hominis.blastocystis hominis is an intestinal protozoan found worldwide, particularly in developing countries, that may cause gastrointestinal symptoms, including diarrhea. we conducted a hospital-based study to identify clinical factors predictive of diarrhea caused by b. hominis. we studied patients with positive stool samples for b. hominis by formalin ethyl acetate concentration technique at srinagarind hospital, khon kaen university, khon kaen, thailand between 2003 and 2010. patients were divided ...201324050068
blastocystis hominis associated acute urticaria.acute urticaria has many causative factors, which may include infections, medications (nonsteroidal anti-inflammatory drugs, antibiotics, contraceptives and others), insect bites, physical stimuli, allergens or underlying systemic disorders. blastocystis spp, although ubiquitous in developing countries, is rarely implicated in causing disease in the developed world. the authors present a case of acute urticaria caused by blastocystis hominis (protozoon parasite) in an elderly farmer in rural uni ...201323360793
prevalence of intestinal protozoa infection among school-aged children on pemba island, tanzania, and effect of single-dose albendazole, nitazoxanide and albendazole-nitazoxanide.pathogenic intestinal protozoa infections are common in school-aged children in the developing world and they are frequently associated with malabsorption syndromes and gastrointestinal morbidity. since diagnosis of these parasites is difficult, prevalence data on intestinal protozoa is scarce.201323289920
hookworm with hypereosinophilia: atypical presentation of a typical disease.we describe a 55-year-old man returning from the philippines infected with a hookworm, the novel bacterium laribacter hongkongensis, and a blastocystis hominis and presenting with both gastrointestinal and neurological symptoms. the high eosinophilia caused by the hookworm infection resulted in both gastrointestinal and neurological symptoms, resembling a hypereosinophilic syndrome.201323809080
protozoa as a cause of recurrent abdominal pain in children.the aim of this study was to investigate whether protozoa can be identified as a cause of recurrent abdominal pain (rap), and whether protozoan infections can be recognized by a specific clinical presentation.201323783023
development of loop-mediated isothermal amplification for rapid detection of entamoeba histolytica.to develop a loop-mediated isothermal amplification (lamp) assay for the detection of entamoeba histolytica e. histolytica, the causative agent of amebiasis.201323711706
[investigation on the infection of blastocystis hominis in populations in bama yao autonomous county of guangxi].497 fecal specimens were collected from 5 randomly selected villages of bama county in december 2011, and tested for blastocystis hominis infection using improved centrifugal sedimentation with hydrochloric acid-ether. data were analyzed by villages, gender, occupation, age groups and ethnic populations. the results showed that 215 people of 497 were positive, with a prevalence of 43.3% (215/497). pandang village had the highest infection rate of 55.7% (68/122), significantly higher than the oth ...201324812846
intestinal parasitic infections in suburban government schools, lak hok subdistrict, muang pathum thani, thailand.to provide baseline information of parasitic infections in 3 suburban government schools, lakhok subdistrict of muang pathum thani, thailand.201323827146
outbreak of gastrointestinal illness during operation new horizons in pisco, peru, july 2012.in july 2012, the u.s. naval medical research unit no. 6 investigated an outbreak of gastrointestinal illness characterized by diarrhea among u.s. service members participating in operation new horizons in pisco, peru. overall, there were 25 cases of self-reported diarrheal illness among 101 respondents to a questionnaire (attack rate: 24.8%). personnel who consumed food that was prepared at the two hotels where they were lodged were more likely to report diarrhea than those who did not eat at t ...201223231049
[blastocystis hominis in symptomatic celiac patients].celiac disease (ec) not diagnosed or treated affect histological, immunological and nutritional status of patients who suffer it. these changes allow infection by parasites that cause no symptoms in immunocompetent patients, such as blastocystis hominis (bh). objetive. to analyze the presence of bh in symptomatic celiac patients and describe the clinical, histological, immune and nutritional status in these patients. material and method an observational descriptive cross sectional study was perf ...201223214346
infections of blastocystis hominis and microsporidia in cancer patients: are they opportunistic?chemotherapy can cause immunosuppression, which may trigger latent intestinal parasitic infections in stools to emerge. this study investigated whether intestinal parasites can emerge as opportunistic infections in breast and colorectal cancer patients (n=46 and n=15, respectively) undergoing chemotherapy treatment. breast cancer patients were receiving a 5-fluorouracil/epirubicin/cyclophosphamide (fec) regimen (6 chemotherapy cycles), and colorectal cancer patients were receiving either an oxal ...201222340948
prevalence of strongyloides stercoralis and other intestinal parasitic infections among mentally retarded residents in central institution of southern iran.to determine the prevalence of intestinal parasitic infections among mentally retarded residents of rehabilitation center of bandar abbas, hormozgan province, southern iran.201223569874
effects of symptomatic and asymptomatic isolates of blastocystis hominis on colorectal cancer cell line, hct116.the pathogenesis of blastocystis hominis in human hosts has always been a matter of debate as it is present in both symptomatic and asymptomatic individuals. a recent report showed that b. hominis isolated from an asymptomatic individual could facilitate the proliferation and growth of existing cancer cells while having the potential to downregulate the host immune response. the present study investigated the differences between the effects of symptomatic and asymptomatic derived solubilized ant ...201222278727
[survey on blastocystis hominis infection in hiv positive individuals in fuyang city, anhui province].to understand the epidemiological characteristics of co-infection of hiv and blastocystis hominis and its risk factors.201223012954
[genotype analysis and isoenzyme patterns of ten isolates of blastocystis hominis from guangxi].to analyze genotypes and lactate dehydrogenase (ldh) and esterase (est) patterns in 10 isolates of blastocystis hominis collected from guangxi.201222913187
[monthly distribution of intestinal parasites detected in a part of western turkey between may 2009-april 2010-results of acid fast and modified trichrome staining methods].in this study, 5073 fecal specimens and cellophane tapes from patients were examined during the period of may 1, 2009-april 30, 2010 in the parasitology laboratory of the ege university medical school.201222801909
blastocystis hominis and endolimax nana co-infection resulting in chronic diarrhea in an immunocompetent male.blastocystis hominis and endolimax nana exist as two separate parasitic organisms; however co-infection with the two individual parasites has been well documented. although often symptomatic in immunocompromised individuals, the pathogenicity of the organisms in immunocompetent subjects causing gastrointestinal symptoms has been debated, with studies revealing mixed results. clinically, both b. hominis and e. nana infection may result in acute or chronic diarrhea, generalized abdominal pain, nau ...201222740811
a hospital-based study of epidemiological and clinical data on blastocystis hominis infection.blastocystis hominis is a foodborne protozoan found in the human feces worldwide. one hundred and ninety-nine individuals with stool samples positive for b. hominis were identified from a pool of 14,325 patient stools collected between 2003 and 2010 from srinagarind hospital in thailand. the medical records of patients were reviewed for demographic and clinical data. of the 85 patients (42.7%) who had b. hominis infection with no co-infections, 42.5% experienced gastrointestinal symptoms. abdomi ...201223075461
epidemiological survey of the prevalence of intestinal parasites among schoolchildren in sari, northern iran.parasitic infection is highly prevalent throughout the developing countries of the world. research on the prevalence of intestinal parasitic infections in various geographic regions is a prerequisite for the development of appropriate control strategies. a cross-sectional study was conducted to determine the prevalence of intestinal parasitic infections among schoolchildren in public primary and secondary schools in the urban areas of sari, mazandaran province, northern iran. the study was condu ...201222703897
blastocystis hominis and recurrent megacolon: a causative or fortuitous association? 201222472370
opportunistic parasites among immunosuppressed children in minia district, egypt.a total of 450 stool samples were collected from inpatient and outpatient clinics of pediatric department, minia university hospital, minia district, egypt. two groups of patients were studied, including 200 immunosuppressed and 250 immunocompetent children. stool samples were subjected to wet saline and iodine mounts. a concentration technique (formol-ether sedimentation method) was carried out for stool samples diagnosed negative by wet saline and iodine mounts. samples were stained by 2 diffe ...201222451735
[the distribution of intestinal parasites in people admitted to the yüzüncü yıl university parasitology laboratory of health research and training hospital, in 2009].this study was performed to present the distribution of intestinal parasites in parients admitted to the parasitology laboratory of the health research and training hospital of yüzüncü yıl university in 2009.201222801916
[survey on co-infection with hiv and intestinal parasites in high prevalence areas of hiv/aids, china].to understand the co-infection status of hiv and intestinal parasites in a hiv/aids highly endemic area in china.201222799161
a study on parasites in mexican rheumatic disease patients.blastocystis hominis is a common human parasite with infection rates up to 50% in developing countries, and giardiasis is the commonest intestinal one in mexico. no doubt, various parasites as giardia lamblia and entamoeba histolytica can cause rheumatic diseases. this study coproparasitoscopic analysis evaluated the cysts by b. hominis, g. lamblia, e. hartmani, e. coli and e. histolytica in mexican rheumatic disease patients. also, elisa was used to detect e. histolytica, ascaris lumbricoides, ...201223214207
intestinal parasite infections in immigrant children in the city of rome, related risk factors and possible impact on nutritional status.parasitic diseases can represent a social and economic problem among disadvantaged people--even in developed countries. due to the limited data available concerning europe, the aims of the present study were to evaluate the presence of parasites in immigrant children and the risk factors favouring the spread of parasites. subsequently, the possible correlation between nutritional status and parasitic infections was also investigated.201223168023
determining the prevalence of intestinal parasites in three orang asli (aborigines) communities in perak, malaysia.this study was conducted to determine the prevalence of intestinal parasites among children and adult orang aslis (aborigines) from different locations in perak. faecal samples were collected and analyzed using the direct smear and formal ether sedimentation technique. some of the faecal samples were stained using the modified acid fast stain for cryptosporidium. nail clippings of the respondents and the soil around their habitat were also analyzed. of the 77 stool samples examined, 39 (50.6%) w ...201222735840
diagnosis of soil-transmitted helminthiasis in an amazonic community of peru using multiple diagnostic techniques.an observational descriptive study was conducted in a shipibo-conibo/ese'eja community of the rainforest in peru to compare the kato-katz method and the spontaneous sedimentation in tube technique (sstt) for the diagnosis of intestinal parasites as well as to report the prevalence of soil-transmitted helminth (sth) infections in this area. a total of 73 stool samples were collected and analysed by several parasitological techniques, including kato-katz, sstt, modified baermann technique (mbt), a ...201222515992
co-infection of hiv and intestinal parasites in rural area of china.intestinal parasite infections (ipis) are among the most significant causes of illness and disease of socially and economically disadvantaged populations in developing countries, including rural areas of the people's republic of china. with the spread of the human immunodeficiency virus (hiv) among rural chinese populations, there is ample scope for co-infections and there have been increasing fears about their effects. however, hardly any relevant epidemiological studies have been carried out i ...201222330320
parasitic prevalence in a suburban school of famaillá, tucumán, argentina.prevalence of intestinal parasites was investigated in rural primary school children in famaillá city, tucumán province, argentina. stool specimens from 149 school children were collected. the prevalence rate of intestinal parasite infections was 86.6%. no significant differences were observed in the distribution by age or by sex. blastocystis hominis was the most commonly found protozoan parasite (54.4%), followed by entamoeba coli (35.6%), giardia lamblia (24.8%), and others (16.7%). enterobiu ...201223724325
broad distribution of tpi-gapdh fusion proteins among eukaryotes: evidence for glycolytic reactions in the mitochondrion?glycolysis is a central metabolic pathway in eukaryotic and prokaryotic cells. in eukaryotes, the textbook view is that glycolysis occurs in the cytosol. however, fusion proteins comprised of two glycolytic enzymes, triosephosphate isomerase (tpi) and glyceraldehyde-3-phosphate dehydrogenase (gapdh), were found in members of the stramenopiles (diatoms and oomycetes) and shown to possess amino-terminal mitochondrial targeting signals. here we show that mitochondrial tpi-gapdh fusion protein genes ...201223284996
intestinal protozoan parasitic infection among school children.intestinal protozoan parasitosis is highly prevalent among general population, majority of them are children. the objective of the study is to find out the prevalence of intestinal protozoan infection in school children of sindhuli.201223281452
intestinal parasitosis among yemeni patients with cancer, sana'a, yemen.the profile of intestinal parasitosis was assessed among patients on anticancer chemotherapy in sana'a city, yemen during the period from april to december 2011. a total of 206 patients (115 males & 91 females), aged 3 to 18 years with mean of 14.17 +/- 3.13 were subjected to stool examinations by different techniques. the overall rate of intestinal parasites was 63.1%. cryptosporidium parvum was the highest (30.1%) followed by g. lamblia (18.0%) and then c. cayetanensis (5.3%). blastocystis hom ...201223469646
isospora belli in a patient with liver transplantation.isospora belli is an opportunistic protozoon which should be monitored in patients with gastrointestinal complaints such as abdominal pain, nausea and diarrhoea, in both immune-compromised and immune-competent patients. our case was a 35 year-old male patient who had received a liver transplant because of cirrhosis and hepatic fibrosis. a diarrhoeic stool sample of the patient was sent to the laboratory for microbiological and parasitological analyses. faecal occult blood was positive and bacter ...201223339948
a parasitological survey of natural water springs and inhabitants of a tourist city in southeastern brazil.abstract the goals of this study were to investigate the occurrence of cryptosporidium oocysts and giardia cysts in main springs of campos do jordão, an important tourist city, in brazil and to gather the largest amount of parasitological data from autochthonous population that live in rural areas of this city. the membrane filtration technique followed by direct immunofluorescence assay was employed for concentration and visualization of waterborne protozoa. in the period between june 2003 an ...201222217166
[expressions and significance of il-17 and il-23 in intestinal mucosa of mice infected with blastocystis hominis].to study the expressions of interleukin-17 (il-17) and interleukin-23 (il-23) in the intestinal mucosa of babl/c mice infected with blastocystis hominis.201223593840
intestinal parasitic infections in hosted saharawi children.literatures on intestinal parasitic infections in saharawi children were scarce and distributed in non parasitological journals. this was the first article that specifically highlighted on the prevalence of these infections in 270 saharawi children aged from 6 to 12 years hosted in spain. six different intestinal parasites were identified in this study and 78, 46, 40, 24, 13 and 5 were positive for giardia lamblia (29%), entamoeba coli (17%), blastocystis hominis (15%), endolimax nana (9%), hyme ...201122433884
prevalence of microsporidium and other intestinal parasites in children from malatya, turkey.parasite infections are common during the critical developmental period in children. the occurrences of intestinal parasites are also common in orphanage, nurseries and schools in turkey. the study was carried out to determine the percentage of microsporidium and intestinal parasites in children from malatya, turkey. this study was carried out at the department of parasitology of inonu university, turgut ozal medical center, during january-december 2006. totally, 1,181 stool samples were examine ...201122754014
enteric protozoan parasites in rural areas of bandar-abbas, southern iran: comparison of past and present situation.the main goal was to address the prevalence of enteric protozoan parasites in rural areas of bandar-abbas, southern iran and to compare the results with the only conducted study in 1978.201123113059
in vitro culture of blastocystis hominis in three liquid media and its usefulness in the diagnosis of blastocystosis.blastocystis hominis is a common protozoan in the human intestinal tract and can cause the so-called blastocystosis characterized by diarrhea. to date, its identification has depended on the discovery of vacuolar, granular, amoebic, or cystic forms in stool samples using wet mount smears, iodine staining, trichrome staining, or iron hematoxylin staining. the permanent staining methods provide more positive findings. however, mercuric chloride (hgcl(2))-based polyvinyl alcohol (pva) and schaudinn ...201122047715
a study on blastocystis hominis in food-handlers: diagnosis and potential pathogenicity.proper diagnosis of blastocystis hominis in not performed routinely in medical laboratories of developing countries; consequently clinical significance of this common intestinal protozoon is liable to remain unsettled. food-handlers are more prone to get and transmit this feco-oral infection. this work compared the sensitivity of direct diagnostic methods to detect b. hominis in stool, estimate the true prevalence among food-handlers in sirte-libya, to clarify the association between the parasit ...201121980782
[Reactive arthritis caused by Blastocistys hominis.] 201122178255
the pathogenic role of different blastocystis hominis genotypes isolated from patients with irritable bowel syndrome.the pathogenic role of blastocystis hominis is still regarded by some as controversial. studies have been in progress for years to evaluate the role of blastocystosis in irritable bowel syndrome (ibs) and demonstrated that faecal carriage of b. hominis was frequent in these patients. this study attempted to distinguish different genotypes of b. hominis isolates obtained from patients with ibs and to evaluate their pathogenic potentials.201122305500
waterborne transmission of protozoan parasites: review of worldwide outbreaks - an update 2004-2010.the present update gives a comprehensive review of worldwide waterborne parasitic protozoan outbreaks that occurred and were published globally between january 2004 and december 2010. at least one hundred and ninety-nine outbreaks of human diseases due to the waterborne transmission of parasitic protozoa occurred and were reported during the time period from 2004 to 2010. 46.7% of the documented outbreaks occurred on the australian continent, 30.6% in north america and 16.5% in europe. cryptospo ...201122048017
No Advantage For Antibiotic Treatment Over Placebo In Blastocystis Hominis-Positive Children With Recurrent Abdominal Pain.OBJECTIVES:: The aim of the study was to investigate if recurrent abdominal pain (RAP) in Blastocystis hominis (B. hominis)-positive children can be treated successfully with trimethoprim-sulfamethoxazole (TMP/SMX). METHODS:: From October 2004 to December 2008 all patients referred to the Division of Gastroenterology and Nutrition of the University Children's Hospital Zurich because of RAP, and detection of B. hominis in stool samples as the only pathological finding after a standard work up, we ...201122002479
Opportunistic and other intestinal parasites among HIV/AIDS patients attending Gambi higher clinic in Bahir Dar city, North West Ethiopia.To determine the magnitude of opportunistic and non-opportunistic intestinal parasitic infections among HIV/AIDS patients in Bahir Dar.201121914548
Intestinal Parasite Screening in Internationally Adopted Children: Importance of Multiple Stool Specimens.Objective: Our goal was to determine the prevalence of intestinal parasites in internationally adopted children, to examine factors associated with infection, and to determine if evaluating multiple stool specimens increases the yield of parasite identification. Methods: We evaluated internationally adopted children with at least 1 stool specimen submitted for ova and parasite testing within 120 days after arrival to the United States. In children submitting 3 stool specimens, in which at least ...201121824880
[the distribution of intestinal parasites detected in the uludag university medical school hospital between 2005 and 2008].objective: intestinal parasite infection is still an important public health problem. in this study, patients (outpatients and in-patients) with various gastrointestinal system complaints presenting at various clinics of the uludag university medical faculty, from january 2009-december 2010 were investigated retrospectively for intestinal parasites. methods: parasitological examinations were done with native-lugol, cellophane-tape methods and trichrome staining in ambiguo ...201122198917
study of blastocystis hominis isolates in urticaria: a case-control study.blastocystis hominis is a common intestinal parasite, with a prevalence in developing countries of up to 50%. the aim of this study was to investigate the association of this parasite with urticaria by determining the genotypic isotypes in the egyptian population. in total, 54 patients with urticaria and 50 controls were enrolled in the study. stool samples were examined and assessed by pcr. the parasite was detected in a significantly higher number (p < 0.001) of the patient group than the cont ...201121790724
blastocystis hominis as a contributing risk factor for development of iron deficiency anemia in pregnant women.intestinal parasitic infection increases the risk of developing iron deficiency anemia (ida) during pregnancy. the objective of this study was to assess blastocystis hominis as a contributing risk factor for development of ida in pregnant women. a total of 200 fecal specimens from 120 pregnant women with ida (mean hb = 9.6 g/dl), and 80 non-anemic controls were examined for blastocystis. fecal specimens were examined by the formalin/ethyl-acetate concentration technique, iron hematoxylin stainin ...201122167376
comparison of intestinal parasitic infection in newly arrived and resident workers in qatar.abstract:201122053720
gastrointestinal parasites of captive and free-roaming primates at the afi mountain primate conservation area in calabar, nigeria and their zoonotic implications.a study on the gastrointestinal parasites among free-living and captive primates at the afi mountain, primate conservation area in calabar, nigeria was undertaken for the first time to ascertain their zoonotic implications. faecal samples were subjected to direct smear, floatation, quantitative estimation of helminth eggs (epg) and oocysts (opg), larval isolation and identification by modified baerman's technique and oocyst sporulation for specie identification. out of the 108 primates examined, ...201122308652
an unusual case of invasive blastocystis hominis infection. 201121590594
[frequency of intestinal parasites among administrators and workers in sanitary and non-sanitary institutions.]objective: transmission of parasites generally occurs through fecal-oral means directly from human to human or through receiving eggs and cysts by means of nourishment. the aim of the study was to investigate the frequency of intestinal parasites among administrators and workers in sanitary and non-sanitary institutions. methods: stool specimens were examined using native-lugol, trichrome and acid-fast stains methods. results: 23.7% of the 2443 fecal specimens were found to be positive. the freq ...201121618189
[investigation of intestinal parasites among primary school students in kayseri-hacilar].objective: parasitic infections are an important health problem which affect children more than adults. especially in growth-age children, this leads to problems such as malnutrition, malabsorption, growth retardation and learning disabilities. in this study, 328 students who were investigated in two primary schools between the ages of 6 and14 in kayseri-hacilar region were analyzed for intestinal parasites. methods: stool samples were analyzed by light microscopy for the detection of helminths ...201121776595
[the epidemiology research of blastocystis hominis in the dokuz eylül university medical faculty hospital between 2005 and 2009].objective: an investigation of blastocystis hominis (b. hominis) prevalance in 17756 patients with gastrointestinal system complaints who presented at the parasitology laboratory of the dokuz eylul university medical faculty hospital between january 2005 and december 2009 was carried out. methods: fecal samples of all patients were examined using the native-lugol and trichrome and kinyoun acid-fast staining method after sedimentation in fecal concentration tubes. results: one or more parasites w ...201121776590
intestinal parasites isolated in a large teaching hospital, italy, 1 may 2006 to 31 december 2008.intestinal parasites account for the majority of parasitic diseases, particularly in endemic areas. most are transmitted via contaminated food. because of increased immigration and travel, enteric parasitoses are now distributed worldwide. between may 2006 and december 2008, we examined stool specimens from 5,351 patients (4,695 italians, 656 non-italians) for ova and parasites using microscopy, culture techniques, and molecular methods. stools from 594 patients (11.1%) were contaminated and for ...201121699767
epidemiological, clinical, and diagnostic data on intestinal infections with entamoeba histolytica and entamoeba dispar among returning travelers.background: among travelers returning from the tropics, entamoeba spp. are among the most frequently detected intestinal parasites, mainly the presumable apathogenic e. dispar and the pathogenic e. histolytica. methods: among 5,378 travelers seeking diagnosis and treatment for intestinal infections at the travel clinic of the university of munich between 2005 and 2009, 103 laboratory-confirmed amebiasis cases were detected. the study compares the results of various diagnostic tests among these p ...201121717146
comparison of the flotac-400 dual technique and the formalin-ether concentration technique for diagnosis of human intestinal protozoon infection.there is a need for accurate diagnosis of intestinal parasite infections in humans, but currently available copromicroscopic techniques have shortcomings, such as low sensitivity. we compared the diagnostic accuracy of a further modified version of the recently developed flotac technique with that of the widely used formalin-ether concentration technique (fect) for the detection of intestinal protozoa in human stool samples. formaldehyde-preserved stool samples from 108 individuals in côte d'ivo ...201121525226
profiles of enteropathogens in asymptomatic children from indigenous communities of mérida, venezuela.in latin america, gastrointestinal infections represent one of the main causes of death among indigenous groups, with a mortality rate three times greater than in the general population. in this study, the carrier state of enteropathogens and the epidemiological risk factor in asymptomatic children from indigenous communities of mérida, venezuela, were determined.201121537069
[blastocystis hominis, brumpt 1912 is a mysterious organism belonging to intestinal protozoa]. 201121797069
ultrastructural insights into morphology and reproductive mode of blastocystis hominis.to understand well the morphology and reproductive mode of blastocystis hominis, with the help of transmission electron microscopy and scanning electron microscopy the ultrastructural details of b. hominis from fresh diarrheal specimens and cultured strains were observed. in both fecal samples and culture conditions, there were vacuolar and granular forms. in diarrhea, it exists in multivacuolar, avacuolar, and amoeboid forms. in the in vitro culture, vacuolar form could transform to granular fo ...201121845408
prevalence and associated factors of intestinal parasitisation: a cross-sectional study among outpatients with gastrointestinal symptoms in catalonia, spain.the aim of this study was to report the prevalence of intestinal parasites in stool specimens from outpatients in catalonia (spain), and to evaluate the association of age, seasonality, and gender on general parasitisation and by the most frequent detected species. a total of 13,913 samples from 8,313 patients (1-3 specimens per patient) reporting digestive disorders were examined between 1999 and 2005 as a part of medical examinations. samples were fixed with mif solution and microscopically ex ...201120862495
development of a new pcr protocol to detect and subtype blastocystis spp. from humans and animals.blastocystis spp. is commonly found in the feces of humans worldwide. infection has been reported as asymptomatic, acute symptomatic, and chronic symptomatic. this wide range of responses to infection could be related to the genetic diversity of morphologically indistinguishable specimens obtained from infected hosts. the former name blastocystis hominis is now reported as blastocystis spp. because of its genetic diversity. blastocystis is recognized as a complex of subtypes that have not been f ...201121210149
proteaese activity of blastocystis hominis subtype3 in symptomatic and asymptomatic patients.despite accumulating evidence indicating that blastocystis hominis is pathogenic and that cysteine proteases are involved in its pathogenesis, few researches discussed the protease activity of b. hominis genetic subtypes. therefore, the present study aims to identify the underlying pathogenic role of the proteases of b. hominis subtype 3 at different molecular weights in correlation to gastrointestinal symptoms. of 65 patients with various clinical presentations referred to our laboratory for st ...201121279383
parasitic infection of the digestive tract in children in a regional hospital center in gharb (kenitra, morroco): some epidemiological features.intestinal parasites are very common in children. we propose to study the prevalence and epidemiological profile of the port of intestinal parasites in 300 children hospitalized in the regional hospital of gharb area (kenitra, morocco) from june to december 2007. this study identified 11 intestinal parasites. of the 300 children, 157 at least one intestinal have parasitism was 52.3% with 91 boxes of poly-parasitism. among the intestinal protozoan identified, is the first amebiasis intestinal par ...201123120932
in vitro sensitivity of blastocystis hominis to garlic, ginger, white cumin, and black pepper used in diet.to determine the growth pattern and in vitro susceptibility of blastocystis hominis to metronidazole (mtz), garlic, ginger, white cumin, and black pepper. stool specimens were collected from 16 irritable bowel syndrome (ibs) and 10 controls between july-november 2010. stool microscopy and culture for b. hominis was performed. drug susceptibility assays was done using 0.01 and 0.1 mg/ml of mtz, garlic, ginger, white cumin, and black pepper. effect was assessed on b. hominis culture after 48 h. st ...201121431384
detection of microsporidia in local hiv-positive population in malaysia.the hiv-positive population, due to their immuno-compromised nature, is considered more susceptible to parasitic infections than other populations. however despite the reports of other opportunistic pathogens such as cryptosporidium and tuberculosis reported in vulnerable communities, microsporidia have not been highlighted in the local hiv-positive population in malaysia. this study aimed to provide preliminary information on the prevalence of microsporidia in the local hiv-population. microspo ...201121596411
[investigation of microsporidia in patients with acute and chronic urticaria].microsporidia species are one of the major causes of severe diarrhea especially in immunocompromised patients, however they also cause infections in immunocompetent subjects. urticaria, which is a common dermatologic disease may be triggered by drugs, infections, foods or food additives, psychogenic factors and autoimmune, metabolic and malignant diseases. while the etiologic grounds of acute urticaria are generally identified, the etiology remains unknown in most of the chronic urticaria cases. ...201121341171
[protozoans in superficial waters and faecal samples of individuals of rural populations of the montes municipality, sucre state, venezuela].in sucre state, the manzanares river is threatened by domestic, agricultural and industrial activities, becoming an environmental risk factor for its inhabitants. in this sense, the presence of protozoans in superficial waters of tributaries of the manzanares river (orinoco river, quebrada seca, san juan river), montes municipality, sucre state, as well as the analysis of faecal samples from inhabitants of towns bordering these tributaries were evaluated. we collected faecal and water samples fr ...201021365874
evaluation of the transmission mode of b. hominis by using pcr method.blastocystis hominis is a common intestinal parasite observed in fecal examination. on the other hand, the transmission of this parasite is certainly unknown. the transmission of b. hominis can be realized by animal contact and the contamination by water and food with excreted cysts from the reservoir hosts. b. hominis isolated from 25 humans, their pets, and tap water was identified by polymerase chain reaction using sequenced tag site primers in this study. b. hominis isolates obtained from hu ...201020544220
clinical efficacy of saccharomyces boulardii or metronidazole in symptomatic children with blastocystis hominis infection.although many blastocystis infections remain asymptomatic, recent data suggest it also causes frequent symptoms. therapy should be limited to patients with persistent symptoms and a complete workup for alternative etiologies. the goal of this study was to compare the natural evolution (no treatment) to the efficacy of saccharomyces boulardii (s. boulardii) or metronidazole for the duration of diarrhea and the duration of colonization in children with gastrointestinal symptoms and positive stool ...201020922415
the potential use of 29 kda protein as a marker of pathogenicity and diagnosis of symptomatic infections with blastocystis hominis.the present study was performed to characterize the protein profiles of blastocystis hominis isolates from symptomatic and asymptomatic individuals by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and immunoblotting using sera from symptomatic and asymptomatic patients. the presence of immunogenic bands associated with pathogenicity or of diagnostic potentials was also evaluated. the study comprised 80 individuals classified into four groups, 20 each: symptomatic blastocys ...201021136081
[blastocystis hominis and nonpathogenic enteric protozoa in patients with pulmonary tuberculosis and those with hiv infection].blastocystis hominis and nonpathogenic enteric protozoa were diagnosed in 300 patients with pulmonary tuberculosis mainly of its infiltrative form and 500 with stages ii and iii hiv infection; the patients received antituberculosis therapy (att) and antiretroviral therapy (art), respectively. control groups included 200 tashkent dwellers and 350 patients with various noninfectious diseases of the gastrointestinal tract. triple coproscopy was made. b. hominis was significantly more frequently det ...201020873179
association of blastocystis hominis genetic subtypes with urticaria.although intestinal parasites are a possible cause of skin disorders, there are few case reports concerning the role of blastocystis hominis in urticaria. to clarify this association, we determined the frequency of b. hominis genetic subtype in urticarial patients by stool culture and polymerase chain reaction (pcr) and evaluated the clinical and parasitological recovery of urticarial patients after treatment with metronidazole. of 54 urticarial patients (group i), 18 (33.3%) were diagnosed as a ...201020922413
changing trends in intestinal parasitic infections among long-term-residents and settled immigrants in qatar.abstract:201020946623
[a retrospective evaluation of the prevalence of intestinal parasites in istanbul, turkey].the aim of this study is to estimate the prevalence of intestinal parasites, in comparison to previous studies in istanbul and different geographic regions of turkey. between january 1999-december 2009, a total of 27664 stool samples from patients with suspicious intestinal parasitic infections who were admitted to istanbul university cerrahpasa medical school hospital were examined for intestinal parasites by using native lugol and formalin-ethyl acetate concentration technique, in adition to t ...201020954117
[investigation of intestinal parasites in students of mustafa cengiz primary school in van].intestinal parasites still maintain as a major public health problem in our country. in this study, we aimed to investigate the distribution of intestinal parasites in 1st and 2nd grade students of mustafa cengiz primary school, aged between 7-9 and to contribute to the parasitological data of our province. for this purpose, stool examinations of a total of 195 students, including 82 boys and 113 girls, were performed. the results of the microscopic analysis of stool samples revealed one or more ...201020954118
[the investigation of intestinal parasites in foreign high school students].intestinal parasites are important health problem especially in undeveloped or underdeveloped countries with low socio-economic status,. in this study, stool and cellophane tape samples were analyzed for intestinal parasites in 192 foreign students who were came from 28 different countries and attending a high school with the age of 15 to 21 (age mean: 17.92 ± 1.30) in kayseri. at least one or more intestinal parasite species were found in 73 (38 %) of them. the distribution of parasites which w ...201020954119
[evolution of the prevalence the enteroparasitoses in talca-chile].the prevalence of intestinal parasites in preschool and school children in the city of talca and rural areas belonging to the maule region, was assessed annually by means of the serial parasitological studies in stool which were performed in the parasitology laboratory of the "universidad de talca". for consecutive periods since 1980 until 2008, an estimated prevalence of parasitism of 76.2% in the population studied was found. these results show a marked decrease from 9.8% to 2.5% in pathogenic ...201021046719
intestinal parasites in immigrants in the city of naples (southern italy).the present study was aimed at carrying out a cross-sectional copromicroscopic survey of helminths and intestinal protozoa in immigrants in naples (southern italy). between october 2008 and november 2009, a total of 514 immigrants were tested comparing the flotac dual technique and the ethyl acetate concentration technique. combined results of the two techniques served as a diagnostic 'gold' standard and revealed an overall prevalence of parasitic infections of 61.9% (318/514). the ethyl acetate ...201021195044
intestinal parasitic infections among intellectual disability children in rehabilitation centers of northern iran.parasitic infection is highly prevalent throughout the developing countries of the world. these infections are the major problem in rehabilitation centers for the mentally retarded. there have been many reports about the prevalence of parasitic infection among different groups of people in iran; however, the epidemiological data in intellectual disability (id) individuals are rare and there are no such data from northern iran. the aim of the present study was to determine the incidence of intest ...201020363588
[isoenzyme patterns of the isolates of blastocystis hominis from guangxi].to observe malate dehydrogenase and alkaline phosphatase patterns of ten isolates of blastocystis hominis (bh) from guangxi.201020411749
[clinical and epidemiological characteristics of blastocystis hominis].blastocystis hominis is an intestinal parasite known since long. it is cosmopolitan and lives in the colon. it is still the subject of controversy regarding its pathogenicity and possibly opportunistic character.201020415193
intestinal parasites infection among immunocompromised patients in riyadh, saudi arabia.this study was conducted to determine the prevalence of intestinal parasites among immunocompromised patients in riyadh, saudi arabia. fecal samples were collected from 136 immunocompromised patients (52 females and 84 males), aged 2 to 69 years. specimens were fixed with 10% formalin for 30 min (2-3 g fecal(-1)) and then concentrated by a formalin-ether sedimentation technique. samples were examined as wet saline mounts and in iodine preparation for detection of protozoan oocysts, cysts, helmin ...201020836300
pathogenic potential of blastocystis hominis in laboratory mice.blastocystis hominis is a ubiquitous enteric protozoan whose pathogenic potential is still controversial. this study was carried out to clarify the pathogenecity of b. hominis infection and to study the proper number of parasites for mice infection. a total of 15 albino mice were orally inoculated with b. hominis and divided according to the inoculums, 10(2), 10(5), and 4 x 10(7) b. hominis forms/100 microl saline, into three groups consisting of five mice each, gi, gii giii, respectively. in ad ...201020499092
blastocystis hominis and dientamoeba fragilis in patients fulfilling irritable bowel syndrome criteria.studies have suggested a possible role for blastocystis hominis and dientamoeba fragilis in the etiology of irritable bowel syndrome (ibs). we studied the prevalence of b. hominis and d. fragilis in patients with ibs-diarrhea (ibs-d). three hundred and thirty patients were enrolled, 171 (52%) with ibs-d and 159 (48%) were controls, respectively. stool microscopy, culture, and polymerase chain reaction (pcr) for b. hominis and d. fragilis were done. b. hominis was positive by stool microscopy in ...201020532564
[the distribution of intestinal parasites detected in the dokuz eylul university medical faculty hospital between 2005 and 2008.]a retrospective evaluation of the data from 14,246 patients with gastrointestinal complaints who presented at the parasitology laboratory of the dokuz eylul university medical faculty hospital between january 2005 and december 2008 was carried out. fecal samples of all patients were examined using native-lugol and the trichrome and kinyoun acid-fast staining method after sedimentation in fecal concentration tubes. one or more parasites were detected in 1320 (9.3%) of the patients. the distributi ...201020340084
intestinal parasitic infection of immunocompromised children with diarrhoea: clinical profile and therapeutic response.parasitic gastrointestinal infections have been variably reported among immunocompromised adults while data on children have been limited. this prospective cross-sectional study aimed to assess the clinical profile of intestinal parasitic infections among immunocompromised children with diarrhoea and their treatment response.201020539063
[prevalence of intestinal parasitosis in reggio emilia (italy) during 2009].the purpose of this survey is to assess the prevalence of intestinal parasites in reggio emilia during 2009. we analyzed 1961 samples from 1503 subjects: a copro-parasitological standard examination was performed in 1416 of the subjects and the scotch-test in the remaining 87. of the 1503 subjects examined, 111 (7.4%) were positive for one or more parasites. pathogenic parasites were found in 44 subjects (39.6% of all positive subjects) and non-pathogenic protozoa were found in 67 subjects (60.4 ...201020956870
controlled study on enteropathogens in travellers returning from the tropics with and without diarrhoea.clin microbiol infect abstract: diarrhoea is the most frequent health problem among travellers in the tropics. however, data on the spectrum and relevance of enteropathogens in international travellers with and without diarrhoea are limited. stool samples from 114 cases of diarrhoea in travellers returning from the tropics were collected for microbiological examination and pcr for norovirus genogroups i and ii, enteroaggregative escherichia coli (eaec), and enterotoxigenic e. coli (etec) produci ...201021054662
six ulcerative colitis patients with refractory symptoms co-infective with blastocystis hominis in china.blastocystis hominis is an enteric parasite which has long been considered as an innocuous commensal living in the intestinal tract. our research was to explore the role of b. hominis in refractory ulcerative colitis. our department admitted 122 cases of ulcerative colitis patients. in these patients, there were 73 cases of patients who were responsive to sulfasalazinec, mesalazine in a standard dosage, according to the symptoms change. there was one patient who was detected to have b. hominis i ...201021104272
intestinal protozoa infections among patients with ulcerative colitis: prevalence and impact on clinical disease course.epidemiological and microbiologic studies suggest that enteropathogenic microorganisms play a substantial role in the clinical initiation and relapses of inflammatory bowel disease.201020145404
irritable bowel syndrome: is it associated with genotypes of blastocystis hominis.blastocystis hominis is the most common intestinal parasite in humans. an extensive genetic variability has been described recently in b. hominis isolates. the aim of this study was to analyze genotypes of b. hominis isolates obtained from the healthy individuals and patients with irritable bowel syndrome-diarrhea (ibs-d). the patients with ibs-d were enrolled from gastroenterology outpatient department at the aga khan university hospital. history and physical examination was done. stool microsc ...201020177906
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