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effect of exogenous adminstration of antioxidants on blastocystis hominis in vivo.the effect of exogenous administration of antioxidant (anttox) on the course of b. hominis in experimentally infected mice was studied. b. hominis isolates were obtained from 10 gastrointestinal symptomatic adult patients. three groups of 30 infected mice (3/isolate) were used. gi was untreated infected, gii was treated by antox for 4 weeks after infection diagnosis (treatment strategy), and giii antox treated by for 4 weeks before infection (prophylactic strategy). mild pathological changes wer ...200819143124
is intestinal parasitic infection still a public health concern among saudi children?to determine the possible health risk associated with intestinal parasite infections among children with gastroenteritis in jeddah, kingdom of saudi arabia.200818998015
cytologic diagnosis of blastocystis hominis in peritoneal fluid: a case report.blastocystis hominis is the most common parasite identified in s worldwide. although it is commonly identified in stool preparations, unusual to encounter b hominis in abdominal fluid.200819068678
clinical and serological evidence of toxocara infection in school children from morrope district, lambayeque, peru.the aim of this study was to estimate the frequency of human toxocariosis in a child population from morrope district, lambayeque, peru. from october to december 2005, 182 school children (96 male and 86 female) were studied. blood samples were collected for toxocara elisa-igg test and hematological examination. additionally, stool samples were collected for coproparasitological examination to check cross reactions. we found frequency of positives in 32.4% (59/182) with a significant higher prop ...200818488089
an outbreak of gastroenteritis associated with intestinal parasites.intestinal protozoa are one of the leading causes of waterborne outbreaks. stool samples of 196 residents from a village of izmir, using the public water supply, were collected during an outbreak of gastroenteritis. patients were asked to fill out a questionnaire reporting on gender, age, gastrointestinal symptoms, whether or not there was a toilet in the house, their hygiene practices, and similar symptoms in the household members. of the patients who had gastrointestinal symptoms (74.5%), diar ...200818985582
[the prevalence of intestinal parasites in children brought to the kars maternal and children's hospital with complaints of gastrointestinal symptoms].this study was carried out to determine the prevalence of intestinal parasites in 2-6 year-old children who were brought to kars maternal and children's hospital with complaints of gastrointestinal symptoms during march-june 2007. fecal samples were taken from children and brought to the parasitology laboratory of the faculty of veterinary medicine to be examined for intestinal parasites. fecal samples were examined by centrifugal formalin ether, zinc-sulphate floatation, and modified acid fast ...200818985583
intestinal parasitic infections in srimum suburban area of nakhon ratchasima province, thailand.a survey on intestinal parasitic infections and some risk factors of infection (social, economic and behavioral) was conducted in suburban area at simum subdistrict, mueang district, nakhon ratchasima province during the period of april 3rd to april 11th, 2007. a total of 214 stool samples, from 85 males and 129 females were examined using simple direct smear, kato's thick smear and modified harada-mori filter paper strip culture technique. the results showed that the prevalence rate of protozoa ...200819287363
[the frequency of intestinal parasites in primary school children in urban and rural regions].the aim of this study was to determine the prevalence of intestinal parasites in primary school children in sivas and to consider the changes in frequency and variety of intestinal parasites in the light of previous studies carried out since 1985. specimens were examined by direct microscopy and staining. in addition, the relationship of intestinal parasites with physical development, success and socio-economic situation was investigated using surveys. intestinal parasites were found in 191 (26. ...200818351553
a possible link between subtype 2 and asymptomatic infections of blastocystis hominis.blastocystis hominis is one of the most common eukaryotic organisms in the intestinal tract of humans, while its pathogenic potential is still controversial. a total of 286 stool samples obtained from adult and pediatric patients with or without gastrointestinal symptoms in two hospitals in manisa, turkey, were cultured to detect b. hominis infection. forty-one and 51 isolates were obtained from the adults and children, respectively, and these isolates were subjected to subtyping by polymerase c ...200818523804
prevalence of intestinal parasites in a population in south of tehran, iran.the aim of this study was to describe epidemiologic characteristics of intestinal parasites in a population in south of tehran, iran. a retrospective cross-sectional study of patients with suspicious intestinal parasitic infections referred to the zakaria razi laboratory in shahre-ray, southern tehran, iran, was conducted from april 21, 2004 to october 20, 2005. all stool samples were examined and socio-demographic informations were retrieved. of 4,371 referred patients, 466 (239 males and 227 f ...200818604414
novel algorithm for coexpression detection in time-varying microarray data sets.when analyzing the results of microarray experiments, biologists generally use unsupervised categorization tools. however, such tools regard each time point as an independent dimension and utilize the euclidean distance to compute the similarities between expressions. furthermore, some of these methods require the number of clusters to be determined in advance, which is clearly impossible in the case of a new dataset. therefore, this study proposes a novel scheme, designated as the variation-bas ...200818245881
giardiasis and other intestinal parasitic infections in relation to anthropometric indicators of malnutrition: a large, population-based survey of schoolchildren in tehran.the morbidity of intestinal parasitic infections is greatest among children of school age and may have an adverse effect on growth. in the present, cross-sectional study, the association between previously undiagnosed intestinal parasitic infections and growth was assessed in 19,209 children attending elementary schools in tehran. the physical growth of these children was investigated by recording body weights, heights and weight-for-age z scores. faecal samples were collected and each checked f ...200818348775
prevalence and factors associated with intestinal parasitic infection among children in an urban slum of karachi.intestinal parasitic infections are endemic worldwide and have been described as constituting the greatest single worldwide cause of illness and disease. poverty, illiteracy, poor hygiene, lack of access to potable water and hot and humid tropical climate are the factors associated with intestinal parasitic infections. the study aimed to estimate prevalence and identify factors associated with intestinal parasitic infections among 1 to 5 years old children residing in an urban slum of karachi pa ...200818997865
patterns of infection with intestinal parasites in qatar among food handlers and housemaids from different geographical regions of origin.intestinal parasitic infections were surveyed among recently arrived immigrant workers in qatar destined for employment in food handling occupations. two overlapping datasets (female workers surveyed in 2005 and 2006, and both sexes in 2006) were analyzed. seven species were detected, 3 nematodes (trichuris trichiura, hookworms and ascaris lumbricoides) and 4 protozoans (entamoeba histolytica/dispar, non-pathogenic entamoebae, blastocystis hominis and giardia lamblia). overall prevalence of infe ...200818457813
is blastocystis hominis a new etiologic factor or a coincidence in iron deficiency anemia?iron deficiency anemia (ida) is a frequent health problem. gut parasites such as n. americanus and a. duodenale are known to cause blood loss, but the role of blastocystis hominis is uncertain. in this study, 212 patients (193 female, 19 male, mean age 41 sd 15 yrs) with ida were enrolled and 90 persons without ida (78 female, 12 male, mean age 45 sd 17 yrs). microscopic examination of stools for b. hominis using the native lugol method was done three times on each subject. if any specimen conta ...200818397391
pathophysiological variability of different genotypes of human blastocystis hominis egyptian isolates in experimentally infected rats.the genotyping of blastocystis hominis clinical isolates obtained from 28 gastrointestinal symptomatic patients and 16 asymptomatic individuals were identified by polymerase chain reaction using sequenced-tagged site (sts) primers. then, pathophysiological variability between different b. hominis genotypes was evaluated in experimentally infected rats. only four b. hominis subtypes (1, 2, 3, and 4) were detected (18.2%, 9.1%, 54.5%, and 18.2%, respectively) in human isolates. in symptomatic isol ...200818193282
extensive multiparasitism in a village of yunnan province, people's republic of china, revealed by a suite of diagnostic methods.intestinal multiparasitism, the accuracy of different diagnostic techniques, and the influence of sampling effort were studied among 215 individuals in a bulang village, yunnan province, people's republic of china. behavioral, demographic, and socioeconomic data were obtained by questionnaire. multiple stool specimens were examined by the kato-katz, koga agar plate, baermann, and ether-concentration methods. eight helminth and 7 protozoa species were diagnosed. the prevalence of each of the 3 ma ...200818458311
[the distribution of intestinal parasites and their causative factors in children].intestinal parasitic infection prevalence in children who had been brought to our hospital together with related factors. the study was carried out in 400 children who were chosen at random among those brought to our hospital. all stool samples were examined with nativelugol, formol-ether, kinyon acid-fast, methods. the cellophane tape preparations and hemoglobin concentration measurement were used. intestinal parasites found in 156 of 400 (39%) children were giardia intestinalis (19.8%) enterob ...200819156610
[the prevalence of microsporidium among patients given a diagnosis of cancer].the aim of this study was to determine the frequency of microsporidium among patients given a diagnosis of cancer. for this purpose fecal samples from 320 patients aged 23.60+/-23.00 years were examined using native-lugol and sedimentation methods and evaluated with modified trichrome, trichrome, and calcofluor dyes. moreover a control group of 320 non-cancer patients was set up. while 10.9% of the patient group was found to have microsporidium, only 5.6% of the control group did. comparison bet ...200818645938
enteroparasitosis and their ethnographic relationship to food handlers in a tourist and economic center in paraná, southern brazil.this study reports on the occurrence of enteroparasites based on data from an ethnographic study of food handlers in the city of cascavel, paraná, brazil. fecal material from 343 food handlers of both sexes, between 14 and 75 years of age, was analyzed using lutz, modified ritchie and ziehl-neelsen techniques. ethnographic relationships were investigated by means of specific questionnaires. positive fecal samples were found for 131 (38.2%) handlers. endolimax nana (67.9%) was the predominant spe ...200919229388
a retrospective analysis of prevalence of gastrointestinal parasites among school children in the palajunoj valley of guatemala.this study retrospectively analyzed demographic factors that may affect the prevalence of intestinal parasites among guatemalan school children. the findings of the study showed that young age, wet season, female gender, and severe malnutrition all correlated positively with increased rates of infection. clinical visits were performed on 10,586 school children aged 5-15 years over a four-year period (2004-2007) in the palajunoj valley of guatemala, during which 5,705 viable stool samples were sc ...200919248646
expression of interferon gamma and proinflammatory cytokines in the cecal mucosa of rats experimentally infected with blastocystis sp. strain rn94-9.blastocystis hominis is a zoonotic intestinal protozoan parasite whose pathogenic potential is still controversial. the aim of the present study was to clarify the pathogenicity of blastocystis parasites in rats. oral inoculation with 1 x 10(5) cysts of blastocystis sp. strain rn94-9 in rats resulted in chronic infection in the cecum at least until 4 weeks after infection. histological examination revealed neither mucosal sloughing nor inflammatory cell infiltration but showed a slight but signi ...200919255785
identification of blastocystis hominis isolates from asymptomatic and symptomatic patients by pcr.despite years of study, the pathogenic role of blastocytis hominis is still controversial. genotypic differences between the asymptomatic and symptomatic isolates should assist in determining the pathogenicity of blastocystis. in this study, we genotyped 32 blastocystis isolates obtained from 12 asymptomatic healthy individuals and 20 symptomatic patients pain by polymerase chain reaction using known seven kinds of sequence tagged site primers in this study. when we compared genotype of blastocy ...200919685075
[prevalence and risk factors associated with intestinal parasitism in preschool children from the urban area of calarcá, colombia].determining intestinal parasite prevalence and their relationship with social and demographic risk factors, hygiene procedures and sanitation in pre-school children.200919721981
[community participation in the control of intestinal parasitoses at a rural site in argentina].to design, implement, and evaluate a complex plan of actions aimed at reducing parasitoses in a rural town in the province of buenos aires, argentina, with the involvement of the community.200920107700
serum leptin concentrations in patients with intestinal parasites.leptin is a protein hormone controlling food intake and energy expenditure. in all infections including parasitic infections there is loss of appetite and anorexia. the aim of the present study was to clarify the relationship between intestinal parasites and serum leptin concentrations in children and adults. forty patients with intestinal parasites and 34 healthy subjects took part in this study. body weight, height and body mass index (bmi) were measured for all patients and controls. patients ...200919851965
study of the dynamics of blastocystis hominis reproduction in vitro.the dynamics of blastocystis hominis reproduction in vitro in pavlova's and nelson-jones media was studied. the time of generation in these media was 21.5 and 16.7 h, respectively. the duration of the lag phase was 1 day, of log phase 2 days, and of the stationary phase 6 days in both cases. the cell count in the logarithmic phase increased at the expense of the vacuolar forms proliferation. during the stationary phase, the granular forms quantitatively predominated over vacuolar forms, the shar ...200919902108
[parasite intestinal infection and factors associated with coccidian infection in adults at public hospital in lima, peru].to evaluate the frequency of intestinal parasites and risk factors associated with coccidian infection in patients cared for at a public hospital in lima-peru.200919915754
giardiasis in kindergartens: prevalence study in berlin, germany, 2006.in germany, an increase of notified giardiasis was observed between 2002 (n = 3,101) and 2007 (n = 3,651) with 62% of cases acquired in germany. the highest incidence was reported in 1- to 5-year-olds (2001-2007, 11.5/100,000 on average) and berlin is one of the most affected states (17.5/100,000). we performed a cross-sectional study in five berlin kindergartens differing in socioeconomic status and migrant proportion in order to estimate the prevalence and investigate routes of transmission am ...200919404678
high prevalence of subtype 4 among isolates of blastocystis hominis from symptomatic patients of a health district of valencia (spain).in order to know the genetic diversity of blastocystis hominis from a health district of valencia (spain) 51 clinical isolates from symptomatic patients, 31 axenic and 20 monoxenic, were ribotyped by analysing the restriction fragment length polymorphism (rflp) of amplicons obtained by polymerase chain reaction (pcr) of small-subunit of ribosomal dna genes (ssu-rdna). for this purpose, dna was subjected to two independent pcr (rd3-rd5, f1-r1) and to three independent treatments with restrictases ...200919471964
blastocystis hominis infection in long-term care facilities in taiwan: prevalence and associated clinical factors.blastocystis hominis is probably the most common protozoan found in the human gut worldwide. in taiwan, the prevalence of b. hominis infection is yet to be determined but is expected to be relatively higher among foreign workers. no data is available on the prevalence of b. hominis infection in long-term care facilities in taiwan. this study included 713 subjects (552 residents and 161 care workers) from ten long-term care facilities in taiwan who completed stool microscopic examinations with me ...200919488784
genetic analysis of blastocystis hominis isolated from symptomatic and asymptomatic human hosts in egypt.extensive genomic polymorphism was demonstrated among morphologically identical b. hominis isolates. a genetic diversity would be a powerful tool for identification or classification of b. hominis subtypes. in this study, 14 egyptian b. hominis isolates were collected, 5 of them were isolated from asymptomatic people whose infections were detected during routine medical check-up and 9 were isolated from patients with gastrointestinal symptoms. restriction fragment length polymorphism (rflp) anal ...200919530613
[blastocystis--an enigmatic parasite].blastocystis can be isolated from roughly 25% of patients suspected of intestinal parasitosis. at least ten subtypes (sts) have been isolated from humans and animals, and recent data demonstrate that the pathogenicity of the parasite is subtype-dependent. for instance, st1 and st7 are more prevalent among patients with symptoms than healthy individuals, whereas st3 predominates among healthy carriers. the article sums up basic aspects of the parasite and gives an introduction to new data and poi ...200919732521
[weight loss]. 200919774510
kinetic analysis of antibody responses to blastocystis hominis in sera and intestinal secretions of orally infected mice.the kinetics of antibody production against blastocystis hominis, an emerging infectious protozoan parasite which causes intestinal disorder in humans and animals, was studied. sera and intestinal secretions were collected from b. hominis-immunized balb/c mice for 8 weeks. flow cytometry was used to monitor the levels of immunoglobulins a (iga), g (igg), and m (igm) from both types of biological samples. the kinetic profile derived from flow cytometry analysis revealed that igm led the early imm ...200919597843
[the effect of trimethoprim-sulfamethoxazole in blastocystis hominis infection].the aim of this study was to investigate clinical findings and the effects of trimethoprim-sulfamethoxazole (tmp-smx) in cases of blastocystosis. a total of 37 cases whose stool specimens were sent to the parasitology laboratory from the outpatient clinics of our hospital for various reasons were included in the study. only five or more blastocystis hominis were found during examination with direct wet mount using the 40x objective. the stool specimens were tested for other agents (salmonella sp ...200920101575
[the role of protozoan parasites in etiology of urticaria].in order to investigate a possible link between parasites and chronic urticaria, the prevalence of intestinal protozoans in stool samples of individuals with chronic urticaria (n=55) and healthy controls (n=43) were evaluated with native lugol, formol-ethyl acetate concentration and trichrom dye, modified acid fast. in the case of giardia intestinalis (g. intestinalis) elisa was also used. in the study 29.1% of the patients were found to have protozoan (blastocystis hominis + g.intestinalis) inf ...200919598090
[intestinal parasitoses detected in primary schoolchildren in the van province].this study was carried out on school children in primary schools in the van province in order to investigate the distribution of intestinal parasitoses. the study was conducted in 7-15 year-old primary schoolchildren during the years from 2003-2005. stool specimens were taken from 2975 school children; of these, 1289 were female and 1686, male. the specimens were examined by native-lugol, flotation and trichrome staining methods in the parasitology laboratory of the health research and training ...200920101580
[intestinal parasites detected in süphan primary schoolchildren in van].this study was performed in order to determine the distribution of intestinal parasites among students of süphan primary school in van in 2005. in the study, stool specimens were taken from 395 students (166 girls and 229 boys) between 7-15 years of age. the specimens were examined by native-lugol, flotation and trichrome staining methods in the parasitology laboratory of health research and training hospital, yüzüncü yil university. one or more parasite species were detected in 28.9 % of the ch ...200920101581
epidemiology of blastocystis hominis and other intestinal parasites among the immigrant population in northeastern taiwan by routine physical examination for residence approval.blastocystis hominis has not been reported as an endemic disease in taiwan, but high prevalence rates have been found among immigrants. due to the increasing number of immigrants in taiwan, b. hominis may become a public health problem in taiwan. this study was performed to determine the prevalence of b. hominis among immigrant populations.200920422136
[the distribution of intestinal parasites among children in denizli].intestinal parasitic disease appears to be an important national health problem in our country. parasitic infection distribution is related to the socioeconomic status and environmental factors. the aim of this study was to determine the distribution of intestinal parasites among children in the 1-15 age group in the denizli province. parasitic infection was observed in 256 (10.2%) out of 2518 children. the highest rate of 31.4% was that of giardia intestinalis. the rates of enterobius vermicula ...200920101582
comment on: frequency of enteric protozoan parasites among patients with gastrointestinal complaints in medical centers of zahedan, iran. 200919307007
high prevalence of microsporidium infection in hiv-infected patients.sixty-four patients infected with human immunodeficiency virus (hiv) participated in a study to determine opportunistic enteric pathogens and compare them with the patients' clinical status. the most frequently found pathogens were microsporidium (81.2%), cryptosporidium parvum (20.3%), candida albicans (12.5%) and blastocystis hominis (10.9%). less frequently found pathogens were giardia intestinalis (6.2%), cyclospora (4.7%), opishorchis viverrini ova (3.1%), strongyloides stercoralis larvae ( ...200919323005
frequency of human toxocariasis in a rural population from cajamarca, peru determined by dot-elisa test.the aim of this study was to estimate the frequency of human toxocariasis in cauday district, cajamarca, peru, using a dot-elisa test. from june to october 2005, a total of 256 adult subjects were studied. blood samples were collected for serology by a dot-elisa test and for hematological examination. parasitological examination was also carried out in stool samples to check cross-reactions in the dot-elisa. the frequency observed was 44.92%, with a significant higher proportion of positivity in ...200919390733
in vivo encystation of blastocystis hominis.blastocystis from infected stools of a person who showed chronic symptoms of abdominal discomfort and diarrhea were examined over a 6-month period, using transmission electron microscopy, for the ultrastructural changes from vacuolar to cystic stage. the study confirms the irregular shedding phenomenon of the organism previously reported, and for the first time, records sequential changes in encystation in stools collected over a time period. the study also confirms the existence of a precystic ...200919238443
intestinal parasitic infections in hiv/aids patients presenting with diarrhoea in jakarta, indonesia.we investigated the occurrence of intestinal parasites in indonesian hiv/aids patients with chronic diarrhoea prior to administering antiretroviral therapy. the influence of age, cd4(+) cell count and season on parasite occurrence was also studied. in total, 318 unconcentrated stool samples were analysed using lugol's iodine and modified acid fast staining to detect intestinal coccidia. most samples (94.5%) were from males aged 21-40 years with cd4(+) counts < or = 50 cells/mm(3). parasites were ...200919327806
[cryptosporidium sp infections and other intestinal parasites in food handlers from zulia state, venezuela].cryptosporidiosis in food handlers from venezuela is unknown, being this an important public health problem in immunosuppressed patients. to determine the prevalence of cryptosporidium sp and other intestinal parasites in food handlers from zulia state, one hundred nineteen fecal samples were evaluated by wet mount, concentrated according to ritchie and modified ziehl-neelsen staining. fourteen (11.8%) were positive for cryptosporidium sp and associated with other protozoosis (p < 0.05), being m ...200919418723
elevated levels of urinary hydrogen peroxide, advanced oxidative protein product (aopp) and malondialdehyde in humans infected with intestinal parasites.oxidative stress has been implicated as an important pathogenic factor in the pathophysiology of various life-threatening diseases such as cancer, cardiovascular diseases and diabetes. it occurs when the production of free radicals (generated during aerobic metabolism, inflammation, and infections) overcome the antioxidant defences in the body. although previous studies have implied that oxidative stress is present in serum of patients with parasitic infection there have been no studies confirmi ...200919154644
intestinal parasitic infections among inhabitants of karaj city, tehran province, iran in 2006-2008.karaj is an area with large influx of refugee people in iran. to increase knowledge about parasitic infections, we carried out this research during 2006-2008. we recorded the stool examination results and some of their personal characteristics. a total of 13,915 human stools were examined, and 649 (4.7%) were positive for intestinal parasites. among them, 13 (0.09%) had worm and 636 (4.6%) had protozoan infections. maximum infections belonged to giardia intestinalis, and 534 (3.8%) samples had t ...200919724700
[intestinal parasitosis in indians of the mapuera community (oriximiná, state of pará, brazil): high prevalence of blastocystis hominis and finding of cryptosporidium sp and cyclospora cayetanensis].occurrences of intestinal parasitosis in indians of the mapuera community (oriximiná, state of pará, brazil) were evaluated. within the context of group assessment, this study makes a contribution towards adequate knowledge of this subject, which is significant from a medical-sanitary point of view. parasitological examination of feces from 83 individuals, performed using four different methods, could be considered to have reasonable amplitude for establishing diagnoses. protozoan cysts and helm ...200919684989
frequency of enteric protozoan parasites among patients with gastrointestinal complaints in medical centers of zahedan, iran.we investigated the prevalence of intestinal protozoan parasites in patients with gastrointestinal complaints in medical centers in zahedan, iran. a total of 1562 stool samples was examined from july 2004 to january 2006 using microscopy (direct smear, formalin-ether concentration), xenic culture and pcr techniques. four hundred and twenty-seven (27.3%) of the patients were infected with one or more intestinal parasites. giardia lamblia (10.1%), entamoeba coli (10%), e. hartmanni (1.7%), blastoc ...200919084249
prevalence of dientamoeba fragilis among patients consulting complementary medicine practitioners in the british isles.a high prevalence of dientamoeba fragilis is reported in faecal samples collected from patients attending complementary medicine practitioners in the british isles. specimens were collected directly after passing into sodium acetate-acetic acid-formalin (saf). during two observation periods in 2002-04 and 2005-07, a d fragilis prevalence of 14.6% (n = 543) and 16.9% (n = 421), respectively, was recorded. these results confirm a surprisingly high prevalence of d fragilis among a selected populati ...200918952691
screening for intestinal parasitic infections among myanmar migrant workers in thai food industry: a high-risk transmission.the impact of intestinal parasitic infections on public health has been neglected. millions of myanmar natives have migrated to work in thailand. we performed a study of intestinal parasitic infections in myanmar-migrants working in the thai food industry. a total of 338 myanmar migrant workers in a food plant at samut sakhon province, thailand, were recruited for this study. 284 (84%) returned requested stool samples. samples were examined for intestinal parasites by means of simple smear, form ...200918815883
microscopic diagnosis of sodium acetate-acetic acid-formalin-fixed stool samples for helminths and intestinal protozoa: a comparison among european reference laboratories.the present study aimed to compare the diagnostic performance of different european reference laboratories in diagnosing helminths and intestinal protozoa, using an ether-concentration method applied to sodium acetate-acetic acid-formalin (saf)-preserved faecal samples. in total, 102 stool specimens were analysed during a cross-sectional parasitological survey in urban farming communities in côte d'ivoire. five saf-preserved faecal samples were prepared from each specimen and forwarded to the pa ...201019456836
[eosinophilia in an adopted native ethiopian girl]. 201019482381
urinary hyaluronidase activity in rats infected with blastocystis hominis--evidence for invasion?the fact whether blastocystis hominis can invade has always been in question. apart from a few sporadic studies such as that done on gnotobiotic guinea pigs which showed surface invasion and mucosal inflammation of the host's intestine caused by b. hominis infection, no real documentation of invasion has been proven. studies have shown that hyaluronidase is secreted during the penetration into the host's skin and gut by nematode parasites. hyaluronidase activity in protozoa namely entamoeba hist ...201020358228
the potential use of 29 kda protein as a marker of pathogenicity and diagnosis of symptomatic infections with blastocystis hominis.the present study was performed to characterize the protein profiles of blastocystis hominis isolates from symptomatic and asymptomatic individuals by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and immunoblotting using sera from symptomatic and asymptomatic patients. the presence of immunogenic bands associated with pathogenicity or of diagnostic potentials was also evaluated. the study comprised 80 individuals classified into four groups, 20 each: symptomatic blastocys ...201021136081
[protozoans in superficial waters and faecal samples of individuals of rural populations of the montes municipality, sucre state, venezuela].in sucre state, the manzanares river is threatened by domestic, agricultural and industrial activities, becoming an environmental risk factor for its inhabitants. in this sense, the presence of protozoans in superficial waters of tributaries of the manzanares river (orinoco river, quebrada seca, san juan river), montes municipality, sucre state, as well as the analysis of faecal samples from inhabitants of towns bordering these tributaries were evaluated. we collected faecal and water samples fr ...201021365874
a case-control study of blastocystis hominis among iranian population.a case-control study was conducted to investigate the epidemiology and clinical features of blastocystis hominis among iranian patients with and without gi symptoms.201021413584
changing trends in intestinal parasitic infections among long-term-residents and settled immigrants in qatar.abstract:201020946623
intestinal parasites in immigrants in the city of naples (southern italy).the present study was aimed at carrying out a cross-sectional copromicroscopic survey of helminths and intestinal protozoa in immigrants in naples (southern italy). between october 2008 and november 2009, a total of 514 immigrants were tested comparing the flotac dual technique and the ethyl acetate concentration technique. combined results of the two techniques served as a diagnostic 'gold' standard and revealed an overall prevalence of parasitic infections of 61.9% (318/514). the ethyl acetate ...201021195044
intestinal parasitic infections among intellectual disability children in rehabilitation centers of northern iran.parasitic infection is highly prevalent throughout the developing countries of the world. these infections are the major problem in rehabilitation centers for the mentally retarded. there have been many reports about the prevalence of parasitic infection among different groups of people in iran; however, the epidemiological data in intellectual disability (id) individuals are rare and there are no such data from northern iran. the aim of the present study was to determine the incidence of intest ...201020363588
[a retrospective evaluation of the prevalence of intestinal parasites in istanbul, turkey].the aim of this study is to estimate the prevalence of intestinal parasites, in comparison to previous studies in istanbul and different geographic regions of turkey. between january 1999-december 2009, a total of 27664 stool samples from patients with suspicious intestinal parasitic infections who were admitted to istanbul university cerrahpasa medical school hospital were examined for intestinal parasites by using native lugol and formalin-ethyl acetate concentration technique, in adition to t ...201020954117
[investigation of intestinal parasites in students of mustafa cengiz primary school in van].intestinal parasites still maintain as a major public health problem in our country. in this study, we aimed to investigate the distribution of intestinal parasites in 1st and 2nd grade students of mustafa cengiz primary school, aged between 7-9 and to contribute to the parasitological data of our province. for this purpose, stool examinations of a total of 195 students, including 82 boys and 113 girls, were performed. the results of the microscopic analysis of stool samples revealed one or more ...201020954118
[isoenzyme patterns of the isolates of blastocystis hominis from guangxi].to observe malate dehydrogenase and alkaline phosphatase patterns of ten isolates of blastocystis hominis (bh) from guangxi.201020411749
[the investigation of intestinal parasites in foreign high school students].intestinal parasites are important health problem especially in undeveloped or underdeveloped countries with low socio-economic status,. in this study, stool and cellophane tape samples were analyzed for intestinal parasites in 192 foreign students who were came from 28 different countries and attending a high school with the age of 15 to 21 (age mean: 17.92 ± 1.30) in kayseri. at least one or more intestinal parasite species were found in 73 (38 %) of them. the distribution of parasites which w ...201020954119
eosinophilia secondary to strongyloides in rhode island. 201020415117
[clinical and epidemiological characteristics of blastocystis hominis].blastocystis hominis is an intestinal parasite known since long. it is cosmopolitan and lives in the colon. it is still the subject of controversy regarding its pathogenicity and possibly opportunistic character.201020415193
intestinal parasitic infection of immunocompromised children with diarrhoea: clinical profile and therapeutic response.parasitic gastrointestinal infections have been variably reported among immunocompromised adults while data on children have been limited. this prospective cross-sectional study aimed to assess the clinical profile of intestinal parasitic infections among immunocompromised children with diarrhoea and their treatment response.201020539063
evaluation of the transmission mode of b. hominis by using pcr method.blastocystis hominis is a common intestinal parasite observed in fecal examination. on the other hand, the transmission of this parasite is certainly unknown. the transmission of b. hominis can be realized by animal contact and the contamination by water and food with excreted cysts from the reservoir hosts. b. hominis isolated from 25 humans, their pets, and tap water was identified by polymerase chain reaction using sequenced tag site primers in this study. b. hominis isolates obtained from hu ...201020544220
geographical location and age affects the incidence of parasitic infestations in school children.environmental factors affect the dissemination and distribution of intestinal parasites in human communities. to comprehend the prevalence of parasitic infestation and to examine whether geographical location and age also influence the prevalence of infection, fecal samples from 195 school children (rural = 95; male = 39; female = 56) (urban = 100; male = 60; female = 40) of five age groups ranging from 5 to 11 years in two different socio-economic zones (rural and urban) were screened for speci ...201020699511
intestinal parasites infection among immunocompromised patients in riyadh, saudi arabia.this study was conducted to determine the prevalence of intestinal parasites among immunocompromised patients in riyadh, saudi arabia. fecal samples were collected from 136 immunocompromised patients (52 females and 84 males), aged 2 to 69 years. specimens were fixed with 10% formalin for 30 min (2-3 g fecal(-1)) and then concentrated by a formalin-ether sedimentation technique. samples were examined as wet saline mounts and in iodine preparation for detection of protozoan oocysts, cysts, helmin ...201020836300
pathogenic potential of blastocystis hominis in laboratory mice.blastocystis hominis is a ubiquitous enteric protozoan whose pathogenic potential is still controversial. this study was carried out to clarify the pathogenecity of b. hominis infection and to study the proper number of parasites for mice infection. a total of 15 albino mice were orally inoculated with b. hominis and divided according to the inoculums, 10(2), 10(5), and 4 x 10(7) b. hominis forms/100 microl saline, into three groups consisting of five mice each, gi, gii giii, respectively. in ad ...201020499092
[prevalence of intestinal parasitosis in reggio emilia (italy) during 2009].the purpose of this survey is to assess the prevalence of intestinal parasites in reggio emilia during 2009. we analyzed 1961 samples from 1503 subjects: a copro-parasitological standard examination was performed in 1416 of the subjects and the scotch-test in the remaining 87. of the 1503 subjects examined, 111 (7.4%) were positive for one or more parasites. pathogenic parasites were found in 44 subjects (39.6% of all positive subjects) and non-pathogenic protozoa were found in 67 subjects (60.4 ...201020956870
[evolution of the prevalence the enteroparasitoses in talca-chile].the prevalence of intestinal parasites in preschool and school children in the city of talca and rural areas belonging to the maule region, was assessed annually by means of the serial parasitological studies in stool which were performed in the parasitology laboratory of the "universidad de talca". for consecutive periods since 1980 until 2008, an estimated prevalence of parasitism of 76.2% in the population studied was found. these results show a marked decrease from 9.8% to 2.5% in pathogenic ...201021046719
[blastocystis hominis and nonpathogenic enteric protozoa in patients with pulmonary tuberculosis and those with hiv infection].blastocystis hominis and nonpathogenic enteric protozoa were diagnosed in 300 patients with pulmonary tuberculosis mainly of its infiltrative form and 500 with stages ii and iii hiv infection; the patients received antituberculosis therapy (att) and antiretroviral therapy (art), respectively. control groups included 200 tashkent dwellers and 350 patients with various noninfectious diseases of the gastrointestinal tract. triple coproscopy was made. b. hominis was significantly more frequently det ...201020873179
[blastocystis hominis infection in a baby with food allergy].the paper describes a rare manifestation of blastocystis hominis infection in a 3-month-old baby with food allergy that appeared as atopic dermatitis and coloproctitis induced by food proteins (cow's milk, hen's egg). the fact that food allergy may be one of the predisposing factors in the development of clinical manifestations of b. hominis infection is discussed. the assumption that the amoeboid form of blastocysts accompanies the clinical manifestation of the infection is confirmed.201020873373
association of blastocystis hominis genetic subtypes with urticaria.although intestinal parasites are a possible cause of skin disorders, there are few case reports concerning the role of blastocystis hominis in urticaria. to clarify this association, we determined the frequency of b. hominis genetic subtype in urticarial patients by stool culture and polymerase chain reaction (pcr) and evaluated the clinical and parasitological recovery of urticarial patients after treatment with metronidazole. of 54 urticarial patients (group i), 18 (33.3%) were diagnosed as a ...201020922413
clinical efficacy of saccharomyces boulardii or metronidazole in symptomatic children with blastocystis hominis infection.although many blastocystis infections remain asymptomatic, recent data suggest it also causes frequent symptoms. therapy should be limited to patients with persistent symptoms and a complete workup for alternative etiologies. the goal of this study was to compare the natural evolution (no treatment) to the efficacy of saccharomyces boulardii (s. boulardii) or metronidazole for the duration of diarrhea and the duration of colonization in children with gastrointestinal symptoms and positive stool ...201020922415
controlled study on enteropathogens in travellers returning from the tropics with and without diarrhoea.clin microbiol infect abstract: diarrhoea is the most frequent health problem among travellers in the tropics. however, data on the spectrum and relevance of enteropathogens in international travellers with and without diarrhoea are limited. stool samples from 114 cases of diarrhoea in travellers returning from the tropics were collected for microbiological examination and pcr for norovirus genogroups i and ii, enteroaggregative escherichia coli (eaec), and enterotoxigenic e. coli (etec) produci ...201021054662
six ulcerative colitis patients with refractory symptoms co-infective with blastocystis hominis in china.blastocystis hominis is an enteric parasite which has long been considered as an innocuous commensal living in the intestinal tract. our research was to explore the role of b. hominis in refractory ulcerative colitis. our department admitted 122 cases of ulcerative colitis patients. in these patients, there were 73 cases of patients who were responsive to sulfasalazinec, mesalazine in a standard dosage, according to the symptoms change. there was one patient who was detected to have b. hominis i ...201021104272
nutritional status, body composition, and intestinal parasitism among the mbyá-guaraní communities of misiones, argentina.indigenous communities in argentina represent socially and economically neglected populations. they are living in extreme poverty and environmental degradation conditions. new information about health status and socio-environmental features is urgently needed to be applied in future sanitary policies. present study describes the nutritional status, body composition, and intestinal parasitism among mbyá-guaraní children from three communities in the misiones province. anthropometric parameters we ...201019642206
[standardization of blastocystis hominis diagnosis using different staining techniques].the present study was carried out from march to may 2008, with the aim of evaluating the effectiveness of different techniques for diagnosing blastocystis hominis in a sample of the population attended at the biomedicine laboratory of feevale university, novo hamburgo, rio grande do sul.201020563502
intestinal protozoa infections among patients with ulcerative colitis: prevalence and impact on clinical disease course.epidemiological and microbiologic studies suggest that enteropathogenic microorganisms play a substantial role in the clinical initiation and relapses of inflammatory bowel disease.201020145404
solubilized antigen of blastocystis hominis facilitates the growth of human colorectal cancer cells, hct116.blastocystis hominis is one of the most common intestinal protozoan parasites in humans, and reports have shown that blastocystosis is coupled with intestinal disorders. in the past, researchers have developed an in vitro model using b. hominis culture filtrates to investigate its ability in triggering inflammatory cytokine responses and transcription factors in human colonic epithelial cells. studies have also correlated the inflammation by parasitic infection with cancer. the present study pro ...201020165878
irritable bowel syndrome: is it associated with genotypes of blastocystis hominis.blastocystis hominis is the most common intestinal parasite in humans. an extensive genetic variability has been described recently in b. hominis isolates. the aim of this study was to analyze genotypes of b. hominis isolates obtained from the healthy individuals and patients with irritable bowel syndrome-diarrhea (ibs-d). the patients with ibs-d were enrolled from gastroenterology outpatient department at the aga khan university hospital. history and physical examination was done. stool microsc ...201020177906
intestinal parasitic infection detected by stool examination in foreign laborers in kaohsiung.most foreign laborers in taiwan come from southeast asia, where public health is not as well funded as in taiwan, and parasitic infections are still common. therefore, we recruited foreign laborers to undergo examination for parasitic infection to determine the infection conditions and follow-up conditions in foreign laborers whose stools were found to be abnormal. a total of 7,360 foreign laborers were enrolled for stool examination in our hospital, and the merthiolate iodine formaldehyde metho ...201020227653
encystation--survival of blastocystis hominis in immunocompetent mice abdomen cavity.human blastocystis hominis were isolated from diarrhea patients' feces and cultured in vitro. then the cultures were inoculated intraperitoneally to laboratory mice. the b. hominis in living mice were collected and inoculated again to healthy mice. the b. hominis showed dose-dependent pathogenicity in the primary inoculation. no pathogenicity was observed in the secondary inoculation. the protozoan existed in the living mice abdomen cavity for more than 6 months and the cyst was the only form. t ...201020306208
[the distribution of intestinal parasites detected in the dokuz eylul university medical faculty hospital between 2005 and 2008.]a retrospective evaluation of the data from 14,246 patients with gastrointestinal complaints who presented at the parasitology laboratory of the dokuz eylul university medical faculty hospital between january 2005 and december 2008 was carried out. fecal samples of all patients were examined using native-lugol and the trichrome and kinyoun acid-fast staining method after sedimentation in fecal concentration tubes. one or more parasites were detected in 1320 (9.3%) of the patients. the distributi ...201020340084
blastocystis hominis and dientamoeba fragilis in patients fulfilling irritable bowel syndrome criteria.studies have suggested a possible role for blastocystis hominis and dientamoeba fragilis in the etiology of irritable bowel syndrome (ibs). we studied the prevalence of b. hominis and d. fragilis in patients with ibs-diarrhea (ibs-d). three hundred and thirty patients were enrolled, 171 (52%) with ibs-d and 159 (48%) were controls, respectively. stool microscopy, culture, and polymerase chain reaction (pcr) for b. hominis and d. fragilis were done. b. hominis was positive by stool microscopy in ...201020532564
high levels of oxidative stress in rats infected with blastocystis hominis.numerous studies have revealed the presence of oxidative stress in parasitic infections. however, such studies were lacking in the malaysian population. previously, we have provided evidence that oxidative stress is elevated in malaysians infected with intestinal parasites. stool examinations revealed that about 47.5% of them were infected with the polymorphic protozoa, blastocystis hominis. however, they were found to have mixed infection with other intestinal parasites.201019961647
intestinal protozoan infections in relation to nutritional status and gastrointestinal morbidity in colombian school children.while giardia duodenalis infection has been consistently associated with nutrient malabsorption and stunting in children, the effects of other protozoans on nutritional status or gastrointestinal morbidity are less clear. we sought to determine whether infection with common intestinal protozoans including giardia duodenalis, entamoeba coli and blastocystis hominis was associated with anthropometric and micronutrient status, gastrointestinal symptoms, visits to the doctor or school absenteeism in ...201020061400
prevalence of intestinal parasitic infections among mentally disabled children and adults of urmia, iran.the prevalence of intestinal parasites infection in institutions for mental retardation of urmia city, west azerbaijan province, iran was investigated.201022347245
parasitic infection in a young man presenting with non-specific abdominal pain.a 27-year-old man presented with a two-week history of central colicky abdominal pain associated with loose stools. further history revealed that he had been exposed to contaminated waters. stool investigation by direct wet stool smears revealed the presence of entamoeba histolytica and blastocystis hominis cysts. a diagnosis of amoebiasis secondary to e. histolytica and concurrent b. hominis infestation was made. we would like to emphasise the importance of clinical history including recent tra ...201025606208
[frequency of intestinal parasites among administrators and workers in sanitary and non-sanitary institutions.]objective: transmission of parasites generally occurs through fecal-oral means directly from human to human or through receiving eggs and cysts by means of nourishment. the aim of the study was to investigate the frequency of intestinal parasites among administrators and workers in sanitary and non-sanitary institutions. methods: stool specimens were examined using native-lugol, trichrome and acid-fast stains methods. results: 23.7% of the 2443 fecal specimens were found to be positive. the freq ...201121618189
[blastocystis hominis, brumpt 1912 is a mysterious organism belonging to intestinal protozoa]. 201121797069
comparison of the flotac-400 dual technique and the formalin-ether concentration technique for diagnosis of human intestinal protozoon infection.there is a need for accurate diagnosis of intestinal parasite infections in humans, but currently available copromicroscopic techniques have shortcomings, such as low sensitivity. we compared the diagnostic accuracy of a further modified version of the recently developed flotac technique with that of the widely used formalin-ether concentration technique (fect) for the detection of intestinal protozoa in human stool samples. formaldehyde-preserved stool samples from 108 individuals in côte d'ivo ...201121525226
prevalence and associated factors of intestinal parasitisation: a cross-sectional study among outpatients with gastrointestinal symptoms in catalonia, spain.the aim of this study was to report the prevalence of intestinal parasites in stool specimens from outpatients in catalonia (spain), and to evaluate the association of age, seasonality, and gender on general parasitisation and by the most frequent detected species. a total of 13,913 samples from 8,313 patients (1-3 specimens per patient) reporting digestive disorders were examined between 1999 and 2005 as a part of medical examinations. samples were fixed with mif solution and microscopically ex ...201120862495
development of a new pcr protocol to detect and subtype blastocystis spp. from humans and animals.blastocystis spp. is commonly found in the feces of humans worldwide. infection has been reported as asymptomatic, acute symptomatic, and chronic symptomatic. this wide range of responses to infection could be related to the genetic diversity of morphologically indistinguishable specimens obtained from infected hosts. the former name blastocystis hominis is now reported as blastocystis spp. because of its genetic diversity. blastocystis is recognized as a complex of subtypes that have not been f ...201121210149
an unusual case of invasive blastocystis hominis infection. 201121590594
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