Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| genomic polymorphism among blastocystis hominis strains and development of subtype-specific diagnostic primers. | genomic polymorphisms among nine strains of blastocystis hominis were examined by random amplified polymorphic dna (rapd) using four different arbitrary polymerase chain reaction (pcr) primers. based on the rapd patterns, nine strains were classified into three groups. the specific primers designed from the unique bands yielded a single band from within each same group, but did not amplify between all the groups examined. specificity of these diagnostic primers was tested against several common ... | 1998 | 9664577 |
| [health examination of children from the democratic sahara republic (north west africa) on vacation in spain]. | we present results of a global health evaluation of groups of children from the democratic sahara republic who came to spain for vacation. | 1998 | 9718764 |
| [blastocystis hominis: the enigma continues]. | 1998 | 9789449 | |
| acute gvhd involving the gastrointestinal tract and infestation with blastocystis hominis in a patient with chronic myeloid leukaemia following allogeneic bone marrow transplantation. | a 21-year-old female underwent allogeneic bone marrow transplantation (abmt) from her hla matched brother for chronic myeloid leukaemia in the chronic phase. four weeks post transplant she developed tenesmus, mucoid stool mixed with blood and lower abdominal pain. this rapidly progressed to greenish watery diarrhoea with flakes of mucous membrane floating in it, conforming to the classical clinical description of acute gvhd of the bowel. stool microscopy showed profuse numbers of blastocystis ho ... | 1998 | 9877276 |
| etiologies of acute, persistent, and dysenteric diarrheas in adults in bangui, central african republic, in relation to human immunodeficiency virus serostatus. | a study of the etiologies of diarrhea in adults in relation to their human immunodeficiency virus (hiv) serostatus and number of cd4+ cells was carried out in the central african republic. in cases and controls, multi-parasitism was observed. salmonella spp. were identified mainly during acute diarrhea, with 50% of the s. enteritidis isolated during the study being responsible for septicemia and/or urinary tract infection in immunodeficient patients. enteroaggregative escherichia coli (eaggec) w ... | 1998 | 9886215 |
| hep-2 cell-adherent escherichia coli and intestinal secretory immune response to human immunodeficiency virus (hiv) in outpatients with hiv-associated diarrhea. | hep-2 cell-adherent escherichia coli and the human immunodeficiency virus (hiv) itself have recently been incriminated as causes of chronic hiv-associated diarrhea. this study sought to determine the prevalence of these two agents among hiv-infected patients with diarrhea in an outpatient setting in the united states and to compare their prevalence to that of other commonly recognized enteropathogens known to be present in this population. hep-2 cell-adherent e. coli was found in 20 of 83 (24.1% ... | 1998 | 9455887 |
| [morphological observation on blastocystis hominis]. | to observe the morphology of blastocystis hominis. | 1998 | 12078221 |
| diagnosis of blastocystis hominis by different staining techniques. | one hundred and fifty stool samples were collected from diarrheic patients of different ages, and examined for blastocystis hominis by direct smears and concentrated by sheather's sugar flotation. staining was done by: giemsa, two modifications of trichrome stain, modified ziehl-neelsen, safranin-methylene blue and two-auramine stains. out of the 150 cases nine were positive for blastocystosis. the best stains were safranin-methylene blue and modified ziehl-neelsen stains. they had the advantage ... | 1999 | 12561895 |
| opportunistic intestinal protozoal infections in immunocompromised children. | hundred immunocompromised children and 100 house contact controls were chosen. patients included: 52 nephrotic syndrome children receiving corticosteroids for more than one month (age 5.28 +/- 2.32 years), 14 protein-calorie malnutrition (pcm) patients (8 cases of marasmus aged 6 +/- 2.27 months and 6 cases of marasmic kwashiorkor aged 1.39 +/- 0.88 years) and 34 lymphomas patients (22 cases of hodgkin's disease and 12 cases of non-hodgkin's lymphoma; age 4.5 +/- 3.54 years). examination of conc ... | 1999 | 12561933 |
| soluble-protein and antigenic heterogeneity in axenic blastocystis hominis isolates: pathogenic implications. | the protein profile and the antigenic cross-reactivity of 18 axenic isolates of blastocystis hominis obtained from symptomatic patients with chronic diarrhea (14 isolates) showing no evidence of parasitic etiology and from patients with acute diarrhea attributable in 2 cases to salmonella spp. were analyzed. sodium dodecyl sulfate-polyacrylamide gel electrophoresis of soluble proteins showed the existence of a common profile composed of 31 bands, with molecular weights ranging between 24 and >20 ... | 1999 | 9934956 |
| development of blastocystis hominis cysts into vacuolar forms in vitro. | the development of cysts of blastocystis hominis isolated from human feces by the ficoll-paque concentration method and cultured in jones' medium containing 10% horse serum is described. the morphological changes were studied by light and transmission electron microscopy at different intervals for up to 48 h. the cysts developed into a large number of vacuolar forms within 24 h, and binary fission was the only mode of reproduction observed. | 1999 | 9934958 |
| irregular shedding of blastocystis hominis. | the shedding pattern of the protozoan parasite, blastocystis hominis, is investigated in man and in experimental animal infections. the shedding pattern of the vacuolar and cystic forms of blastocystis hominis in infected individuals have been shown in the present study to be irregular. the study shows that there is marked fluctuation in the shedding of the parasite from day to day, varying from as high as 17 to 0 per x40 microscopic field. the cystic stages when estimated in 8 blastocystis-infe ... | 1999 | 9934969 |
| characterization of protein profiles and cross-reactivity of blastocystis antigens by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblot analysis. | the protein profiles of blastocystis hominis, b. lapemi, and b. ratti were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and species could be differentiated by this means as well as by western-blot analysis with polyclonal antibodies. no intraspecies difference could be distinguished between the two b. hominis isolates or the three b. ratti isolates. western-blot analysis showed extensive cross-reactivity of b. lapemi and b. hominis antigens with anti-b. ratti ... | 1999 | 10099019 |
| postcystic development of blastocystis hominis. | a concentrated suspension of blastocystis hominis cysts was inoculated into jones' medium and removed after 24 h for study of their development at the ultrastructural level. the parasite divides in the cyst, and up to three daughter cells can be seen. during this process the cyst wall dissolves, leaving behind thin membranous remnants. excystation occurs mostly by the emergence of the daughter cells through an aperture in the outer fibrillar coat. before excystation the vacuolar and the granular ... | 1999 | 10344535 |
| isolate resistance of blastocystis hominis to metronidazole. | isolates of blastocystis hominis from infected immigrant workers from indonesia, bangladesh and infected individuals from singapore and malaysia were assessed for growth pattern and degree of resistance to different concentrations of metronidazole. viability of the cells was assessed using eosin-brillian cresyl blue which stained viable cells green and nonviable cells red. the bangladeshi and singaporean isolates were nonviable even at the lowest concentration of 0.01 mg/ml, whereas 40% of the i ... | 1999 | 10357863 |
| [hymenolepis nana. 45-year-old refugee from the kosovo region with epigastric pain and detection of hymenolepis nana and blastocystis hominis in the stool]. | 1999 | 10409887 | |
| prevalence and clinical relevance of blastocystis hominis in diverse patient cohorts. | the pathogenicity of blastocystis hominis is extensively debated in the medical literature. therefore, we did a prevalence study to investigate the association between the presence of several intestinal parasites and gastrointestinal symptoms in diverse patient cohorts. the study population consisted of 1216 adults, including immunocompromised patients, institutionalized psychiatric or elder subjects, immigrants from developing countries, travellers to developing tropical countries and controls. ... | 1999 | 10414382 |
| irritable bowel syndrome in patients with blastocystis hominis infection. | the prevalence of blastocystis hominis in stool specimens of individuals with gastrointestinal symptoms was evaluated to study a possible link between the protozoan and the irritable bowel syndrome. according to the rome diagnostic criteria, 388 patients were evaluated. altogether, 81 patients were classified as affected by irritable bowel syndrome. blastocystis hominis was recovered from the stools of 38 subjects, 15 of whom belonged to the group with irritable bowel syndrome (p = 0.006). in ad ... | 1999 | 10442423 |
| intestinal blockage by carcinoma and blastocystis hominis infection. | we detected heavy infections of blastocystis hominis in four individuals with intestinal obstruction due to cancerous growths. after surgery, the infections spontaneously resolved, without specific chemotherapy. it appears that the b. hominis infection was coincidental and not related to the neoplastic growth. we suggest that intestinal obstruction and concomitant stool retention, plus hemorrhage from cancerous lesions, may have permitted the more abundant growth of b. hominis. this is the first ... | 1999 | 10466967 |
| intestinal parasites in malaysian children with cancer. | in this prospective study, we examined stool specimens from children with cancer receiving chemotherapy who were admitted for fever to the universiti sains malaysia hospital in kota baru, kelantan. stool specimens were examined for ova and cysts of parasites. over a period of 15 months, there were 129 febrile episodes in 50 children with cancer and, in all, 237 stool specimens were examined. sixty-six per cent of febrile episodes were associated with neutropenia and 9 per cent were associated wi ... | 1999 | 10467838 |
| [seasonal variation of intestinal protozoa infections in outpatients of the north section of santiago, chile. 1995-1996]. | formalin preserved fecal samples from 6,058 and 5,863 outpatients were examined for intestinal parasites during 1995 and 1996 respectively. prevalence rates of infections by intestinal protozoa in both years were similar. by age group (0-9, 10-19 and > 20 years old) blastocystis hominis was observed in 18.6-19.3, 37.0-31.1 and 25.3-25.4% in 1995-1996 respectively. prevalence of giardia intestinalis infections decreased from 16.6-17.4% in the 0-9 year-old children group to 4.1-4.5% in patients ov ... | 1999 | 10488591 |
| [blastocystis hominis: epidemiological and clinical remarks from more than 3,500 stool examinations]. | 1999 | 10518063 | |
| intestinal amebae. | although the intestinal amebae that infect humans are not thought of as classic agents of food-borne disease, food plays an important role in the transmission of these protozoa. this is particularly true for areas of the world where the organisms are endemic. transmission of most intestinal protozoa occurs by the fecal-oral route via contaminated food or water. among the four genera of amebae that infect man, only entamoeba histolytica and blastocystis hominis are causes of disease. this article ... | 1999 | 10549428 |
| effect of trimethoprim-sulfamethaxazole in blastocystis hominis infection. | blastocystis hominis (b. hominis) is a common intestinal parasite that has long been considered nonpathogenic. recently there have been many reports supporting a role for the organism as a potential pathogen. we performed a study to examine the pathogenicity of b. hominis and the effect of trimethoprim-sulfamethaxazole (tmp-smx) on this organism. | 1999 | 10566723 |
| prevalence of intestinal parasitic infections in patients with acquired immunodeficiency syndrome in brazil. | to evaluate the prevalence of intestinal parasitic infections and to investigate the possible associations of clinical status and laboratory findings with the different parasites found in stool samples. | 1999 | 10575149 |
| scanning electron microscopy of the surface coat of blastocystis hominis. | scanning electron microscopy of blastocystis hominis showed that its outer coat has a fibrillar structure and individual fibrils may extend up to 5 microm from the periphery of the parasite. the surface coat remains intact during cell division. bacteria are often seen adhering to it, but for the first time a trophozoite of chilomastix mesnili was also seen in this position. it is postulated that breakdown of attached organisms may provide nutrients for blastocystis. | 1999 | 10599919 |
| blastocystis in animal handlers. | the present study investigated whether people working closely with animals were at higher risk of getting infected with blastocystis hominis. the prevalence of the parasite was determined in two population groups, i.e., animal handlers and normal healthy individuals who did not work with animals. in all, 105 stool samples were collected from animal handlers from 2 local research institutions, a local zoo, and a local abattoir and 163 stool samples were collected from normal healthy individuals r ... | 1999 | 10599928 |
| infestations and chronic infections in foreign pediatric patients with burns: is there a role for specific protocols? | infestations by parasites such as mycobacterium tuberculosis and other viral infections are common in third world countries. consequently, the admission of a significant number of foreign patients to burn centers in the united states may pose new problems, not only for inpatients but also for health care workers. to document infestations in patients from third world countries and to determine the need for specific protocols, we studied 62 consecutive foreign patients admitted to our pediatric bu ... | 1999 | 10613686 |
| intestinal protozoa in hiv-infected patients: effect of rifaximin in cryptosporidium parvum and blastocystis hominis infections. | in hiv-1 infected patients severe enteritis and chronic diarrhea are often documented as a consequence of multiple opportunistic infections. we analyzed 48 hiv-1 positive patients for the presence of intestinal pathogenic protozoa. patients with cd4 > or = 200/mm3 showed a higher prevalence of a single pathogenic protozoa than patients with cd4 < or =200/mm3, who showed the presence of multiple protozoal infections. patients who proved positive for only a single protozoa, cryptosporidium or blas ... | 1999 | 10632386 |
| intestinal protozoa in homosexual men in edinburgh. | our objective was to determine the prevalence of intestinal protozoa in homosexual men attending a sexually transmitted diseases (std) clinic, to compare it with the prevalence in a previous study from the same clinic, and to examine the relationship, if any, between the presence of protozoa and sexual practices. men who consecutively attended the clinic and who gave a history of having had recent sexual contact with another male were invited to provide a stool sample for protozoological examina ... | 1999 | 10639057 |
| [dientamoeba fragilis: pathogenic flagellate?]. | d. fragilis is an intestinal protozoa whose pathogenic characteristics are increasingly recognized. the aim of this study is to specify the epidemiologic, biological and clinical aspects of this protozoa. material used and methodology: survey conducted on 27,058 parasitological test of stools in parasitology-mycology laboratory of the sfax university hospital over a period of 5 years. | 1999 | 10690462 |
| intestinal protozoa in hiv-infected patients in apulia, south italy. | protozoa are important enteric pathogens in patients with human immunodeficiency virus (hiv) infection. in this study the prevalence of intestinal protozoa in 154 hiv-infected patients, with or without diarrhoea, in our region (apulia, south italy) was evaluated between december 1993 and february 1998. in the majority of patients cd4+ t cell count was below 200/microl. the overall prevalence of intestinal protozoa was 43/154 (27.92%). twenty-eight (43.08%) out of 65 patients with diarrhoea and 1 ... | 1999 | 10694157 |
| genomic analysis of blastocystis hominis strains isolated from two long-term health care facilities. | the genotype blastocystis hominis is highly polymorphic. therefore, a genetic marker would be a powerful tool for the identification or classification of b. hominis subtypes and could be used as a means to resolve the transmission route or origin of the parasite. to this end, 32 b. hominis isolates were collected from patients and/or staff members of two long-term health care facilities (facilities a and b), and these organisms were subjected to genotype analysis based on diagnostic pcr primers ... | 2000 | 10747102 |
| comparison of polyvinyl alcohol fixative with three less hazardous fixatives for detection and identification of intestinal parasites. | polyvinyl alcohol (pva) containing the fixative mercuric chloride is considered the "gold standard" for the fixation of ova and parasites in the preparation of permanently stained smears of stool specimens. however, mercuric chloride is potentially hazardous to laboratory personnel and presents disposal problems. we compared three new alternative, nontoxic fixatives with pva, analyzing ease of sample preparation and quality of smears. sixty-eight fresh stool specimens were divided into aliquots ... | 2000 | 10747149 |
| [intestinal parasites in school children at a public institution in maracaibo municipality, venezuela]. | to determine the prevalence and epidemiological parameters of intestinal parasites in schoolchildren, a faecal analysis was performed in 349 individuals of both sexes, between 5 and 16 years of age. the methodology used included, a fresh test, formol-ether technique and faecal egg counts by the kato-katz method. eighty three per cent of the children presented enteroparasites, with a high prevalence of polyparasitism (71.6%). there was not significative difference between parasitoses, sex and age ... | 2000 | 10758698 |
| prevalence of enteric pathogens among community based asymptomatic individuals. | the objective of this study was to describe the prevalence of pathogenic microorganisms in asymptomatic individuals in a community study in melbourne, australia. | 2000 | 10764030 |
| performance of para-pak ultra ecofix compared with para-pak ultra formalin/mercuric chloride-based polyvinyl alcohol for concentration and permanent stained smears of stool parasites. | ecofix is a mercury and formalin-free fecal preservative that can be used for concentration of stool specimens and preparation of permanently-stained slides. in this study, the standard two-vial parapak ultra system was compared with ecofix ultra for the detection of intestinal parasites. a total of 261 specimens in 92 sets (77 with 3 specimens, 15 with 2 specimens) were collected in ecofix, formalin, and low viscosity polyvinyl alcohol (lv-pva). concentrations were performed from ecofix using h ... | 2000 | 10794938 |
| inhibition of enteric parasites by emulsified oil of oregano in vivo. | oil of mediterranean oregano oreganum vulgare was orally administered to 14 adult patients whose stools tested positive for enteric parasites, blastocystis hominis, entamoeba hartmanni and endolimax nana. after 6 weeks of supplementation with 600 mg emulsified oil of oregano daily, there was complete disappearance of entamoeba hartmanni (four cases), endolimax nana (one case), and blastocystis hominis in eight cases. also, blastocystis hominis scores declined in three additional cases. gastroint ... | 2000 | 10815019 |
| enteropathogens in adult patients with diarrhea and healthy control subjects: a 1-year prospective study in a swedish clinic for infectious diseases. | a 1-year prospective study was conducted to identify enteropathogens in adults with diarrhea (n=851) and in healthy control subjects (n=203) by use of conventional laboratory methods. virulence factor genes for diarrheagenic escherichia coli were detected by polymerase chain reaction. enteropathogens were identified in 56% of patients and 16% of control subjects. the isolation rate was 65% for patients with symptoms for <1 week and for travelers; >1 pathogen was found in 11% of patients. the mos ... | 2000 | 10816147 |
| prevalence of enteroparasites in a residence for children in the córdoba province, argentina. | a study of the prevalence of enteroparasites in a population belonging to a substitute home that gives shelter to orphaned and homeless children was done using conventional methods of analysis. this home is located in córdoba province, argentina, and has the following characteristics: it has nine houses located inside the main plot of ground, that shelter 139 individuals, and 25 houses outside this plot distributed randomly in unquillo city and that shelter 257 individuals. the overall parasitic ... | 2000 | 10870946 |
| blastocystis hominis: origin and significance of vacuolar and granular forms. | using supravital neutral red staining and light microscopy, individual blastocystis organisms, subcultured from clinical isolates in modified monophasic robinson's medium, were followed over various periods on glass slides. a rapid transition from uniformly stained to granular and vacuolated forms preceded the organism's death as evidenced by pale staining and brownian motion in the cell's interior. granular and vacuolar forms of blastocystis may indicate degenerative changes in individual cells ... | 2000 | 10669129 |
| restriction-fragment-length polymorphism analysis of small-subunit rrna genes of blastocystis hominis isolates from geographically diverse human hosts. | genomic diversity among 14 isolates of blastocystis hominis from 4 different geographic locations was examined by small-subunit rrna (ssu rrna) restriction-fragment-length polymorphisms (rflp) using 5 different restriction endonucleases. on the basis of the observed rflp patterns among the isolates, a total of 12 genotypes were identified, with 7 isolates exhibiting mixed rflp genotypes. there was no correlation between b. hominis geographic origin and rflp banding pattern or genotype. | 2000 | 10669137 |
| serologic response to blastocystis hominis infection in asymptomatic individuals. | the pathogenic potential of blastocystis hominis in the human intestine is subject to controversy because the organism has been found in both symptomatic and asymptomatic individuals. to help clarify this issue, we monitored the serologic response to the organism in b. hominis-infected individuals free of gastroenterologic disorders. 1) serum antibodies to b. hominis were detected in 70% of infected asymptomatic individuals by an indirect immunofluorescence (ifa) test. 2) ifa and immunoelectron ... | 2000 | 11127507 |
| clinical significance and frequency of blastocystis hominis in turkish patients with hematological malignancy. | the effect of blastocystis hominis (b. hominis) in both immunocompetent and immunocompromised subjects has been the subject of debate in recent years, mostly in response to its unknown pathogenicity and frequency of occurrence. we performed a non-randomised, open labelled, single institute study in our hospital in order to investigate the clinical significance and frequency of b. hominis in patients suffering from hematological malignancy (hm) who displayed symptoms of gastrointestinal diseases ... | 2000 | 10925738 |
| blastocystis hominis: neutral red supravital staining and its application to in vitro drug sensitivity testing. | a simple in vitro drug sensitivity testing system for blastocystis hominis clinical isolates was developed. application of supravital staining by neutral red allowed quantitative viability assessment. four xenic cultures, isolated from human sources, were grown in modified monophasic robinson's medium and tested for sensitivities to nine available drugs. assessment was done using the cell-count method from air-dried preparations after supravital staining with neutral red. also, the light absorbe ... | 2000 | 10935909 |
| identification of enteric pathogens in hiv-positive patients with diarrhoea in northern india. | enteric pathogens associated with chronic diarrhoea in hiv-positive patients were studied. the study was conducted during january 1995-december 1998. stool specimens from all diarrhoea patients (n = 26) were examined microscopically for ova and parasites using wet preparations and stained smears. stool samples from diarrhoea patients were also cultured on appropriate media to isolate enteric bacterial pathogens. of the 59 patients, 26 (44%) had prolonged diarrhoea for more than 4 weeks. enteric ... | 2000 | 11014766 |
| transmission of intestinal blastocystosis related to the quality of drinking water. | a cross-sectional study was performed to evaluate the risk factors of blastocystis hominis infection in the thai army population of the 11th infantry division, chachoengsao province, thailand. 201 army personnel and their family members were enrolled in this study. intestinal parasitic infections in this population were assessed by stool examination using simple smear, formalin/ether technique and kato-thick smear. approximately one third of the specimens were positive for one or more intestinal ... | 2000 | 11023076 |
| blastocystis hominis: a simplified, high-efficiency method for clonal growth on solid agar. | colony growth of protozoan parasites in agar can be useful for axenization, cloning, and viability studies. this is usually achieved with the pour plate method, for which the parasite colonies are situated within the agar. this technique has been described for giardia intestinalis, trichomonas vaginalis, and entamoeba and blastocystis species. extracting such colonies can be laborious. it would be especially useful if parasites could be grown on agar as colonies. these colonies, being exposed on ... | 2000 | 11038315 |
| blastocystis elongation factor-1alpha: genomic organization, taxonomy and phylogenetic relationships. | the elongation factor-1 alpha (ef-1alpha) is a highly conserved ubiquitous protein that is involved in translation and is desirable for use in phylogenetic studies on blastocystis, an enigmatic intestinal parasite with a contentious taxonomic position. in the present study, a pcr product (bealpha) that codes for a major part of the coding region of the ef-lalpha protein was amplified. genome walking experiments together with cloning were implemented to elucidate the 5' and 3' ends of the ef-1alp ... | 2000 | 11085233 |
| a survey on the intestinal parasites of the school children in kaohsiung county. | the present study on the prevalence of intestinal parasites infection, from september to december 1999, was conducted among school children in taoyuan hsiang, kaohsiung county. the investigated areas included three (jiannshan, shingjong and taoyuan) primary schools. the overall infection rate in 305 children determined by merthiolate-iodine-formaldehyde concentration method of stools was 17%. four confirmed species of helminthes (ascaris lumbricoides, hookworm, trichuris trichiura and hymenolepi ... | 2000 | 11271730 |
| comparison of antigen-capture elisa to stool-culture methods for the detection of asymptomatic entamoeba species infection in kafer daoud, egypt. | we performed a prospective field study in the village of kafer daoud in menofia, egypt to compare the fecal culture method with enzyme linked immuno assay (elisa) for detection of 170 kda lectin antigen in feces for diagnosis of asymptomatic entamoeba histolytica and entamoeba dispar infection. all subjects with e. histolytica or e. dispar infection detected by culture also had positive elisa for amebic antigen in their feces and an additional 57 entameoba infections missed by culture were detec ... | 2000 | 11289667 |
| [acute infectious lymphocytosis associated to giardia lamblia and blastocystis hominis coinfection]. | acute infectious lymphocytosis is characterized by marked peripheral blood lymphocytosis, often associated with a mild, nonspecific febrile illness. we present a 4-year-old girl with acute infectious lymphocytosis associated with giardia lamblia and blastocystis hominis coinfection. analysis of peripheral lymphocyte markers showed overall proliferation of b and t cells with a reduction in the proportion of t cells, especially in the cd4t cell subpopulation. hematologic values returned to normal ... | 2001 | 11333485 |
| blastocystis hominis. causative organism or harmless commensal? | 2001 | 11355227 | |
| blastocystis hominis: a new pathogen in day-care centres? | 2001 | 11381629 | |
| do blastocystis hominis colony forms undergo programmed cell death? | ultrastructural observations were made on colony forms of the protozoan parasite, blastocystis hominis. cross-sections of entire colonies were observed by tem. cells within a colony were heterogeneous in morphology, consisting of vacuolar, amoeboid, multivacuolar and unusual forms. dying cells appeared to be in the process of fragmenting into numerous membranebound vesicles, giving rise to empty spaces within the colony. interestingly, older colonies appeared to show cell fragmentation which res ... | 2001 | 11403377 |
| cryptosporidiosis in kuwaiti children: seasonality and endemicity. | to detect the incidence of cryptosporidial infection in children presenting with gastrointestinal symptoms at the local hospital in kuwait. | 2001 | 11422253 |
| blastocystis hominis: a common yet neglected human parasite. | 2001 | 11440205 | |
| morphology, histochemistry and infectivity of blastocystis hominis cyst. | different morphological forms of blastocystis hominis had been identified in human stool samples. these included both cystic and trophic stages. the latter was induced to encyst by keeping them in potassium dichromate solution for two weeks. suspected of being the infective stage, cysts were studied in more detail as regards their morphology using both light and electron microscopy. histochemistry and infectivity studies were also carried out. light microscopy revealed the cysts to be ovoid or r ... | 2001 | 11478461 |
| exposure of blastocystis species to a cytotoxic monoclonal antibody. | a recently described cytotoxic monoclonal antibody (mab 1d5) raised against blastocystis hominis isolate b, was tested for reactivity with 13 different isolates of blastocystis. the isolates used were previously isolated from humans, rats and reptiles and were maintained as axenised cultures throughout the course of this study. five b. hominis isolates (b, c, e, g and h) were found to react with mab 1d5 in immunoblotting studies and the indirect fluorescence antibody test. the pattern of fluores ... | 2001 | 11484849 |
| blastocystis hominis: evidence for caspase-3-like activity in cells undergoing programmed cell death. | we have shown previously that the human intestinal protozoan, blastocystis hominis, undergoes apoptosis-like programmed cell death (pcd) when exposed to a cytotoxic monoclonal antibody (mab), 1d5. in the present study, elisa and immunoblot assays employing chicken anti-cpp32 antibody suggest that caspase-3-like antigens exist in b. hominis. using colorimetric and flow cytometric assays for caspase-3 activity, we also observed an increase in activity between 1 h and 6 h after exposure to mab 1d5, ... | 2001 | 11484854 |
| intestinal parasites in hiv-2 associated aids cases with chronic diarrhoea in guinea-bissau. | previous studies from african countries where hiv-1 infection is prevalent have shown that infections with cryptosporidium parvum, isospora belli and microsporidia are frequently associated with chronic diarrhoea in aids patients. the information about the occurrence of these parasites in hiv-2 associated aids cases with chronic diarrhoea is limited. we have performed a study of stool parasites in patients from guinea-bissau, the country with the highest prevalence of hiv-2 in the world. stool s ... | 2001 | 11495643 |
| programmed cell death in a human intestinal parasite, blastocystis hominis. | although programmed cell death (pcd) has been associated with multicellular organisms, there have been more reports of its presence in some protozoans. our study shows the existence of pcd in an intestinal protozoan, blastocystis hominis. light and electron microscopy, biochemical and flow cytometry studies showed apoptosis-like death in b. hominis cells exposed to a cytotoxic monoclonal antibody (mab 1d5). b. hominis cells displayed key morphological and biochemical features of apoptosis, namel ... | 2001 | 11578087 |
| blastocystis hominis in high-rise flat dwellers in kuala lumpur, malaysia. | 2001 | 11579877 | |
| parasitic infections of the gastrointestinal tract. | this article updates recent advances in the body of knowledge of diagnosis and treatment of intestinal parasites. the articles focus on the manifestations of disease in the immunocompetent adult host from developed countries. specific pathogens discussed are giardia lamblia and dientamoeba fragilis, entamoeba histolytica, entamoeba dipar, blastocystis hominis, cyclospora cayetanensis, and cryptosporidium parvum. | 2001 | 11586558 |
| chronic intermittent diarrhoea and fatigue. | 2001 | 11676320 | |
| ribodemes of blastocystis hominis isolated in japan. | to determine if genetic diversity of blastocystis hominis exists in japan, we monitored 64 b. hominis-infected people: 39 asymptomatic people whose infections were detected during routine medical check-ups (32 japanese and 7 non-japanese) and 25 patients with gastrointestinal symptoms who visited the outpatient clinics of st. luke's international hospital (19 japanese and 6 non-japanese). we detected 6 known and 2 new riboprint patterns in isolates from the infected people. there were no differe ... | 2001 | 11693890 |
| multiyear prospective study of intestinal parasitism in a cohort of peace corps volunteers in guatemala. | we conducted a prospective, longitudinal study in a cohort of 36 peace corps volunteers (pcvs) in guatemala to study the incidence and natural history of intestinal parasitic infections during the pcvs' >2-year overseas stay. pcvs collected stool specimens at least monthly and when ill with gastrointestinal symptoms. of the 1,168 specimens tested, 453 (38.8%) were positive for at least one parasite and 48 (4.1%) were positive for a pathogenic parasite. a median interval of 187 days (range, 14 to ... | 2001 | 11136744 |
| blastocystis hominis modulates immune responses and cytokine release in colonic epithelial cells. | an experimental in vitro model has been developed in order to determine whether blastocystis hominis is able to trigger inflammatory cytokine response in colonic epithelial cells. after 24 h incubation of b. hominis with the cell lines ht-29 and t-84, b. hominis cells were not able to cause cytopathic effects, but significant increases in the release of the cytokines il-8 and gm-csf could be observed. however, after the first 6 h of co-incubation, the production of il-8 was not increased in ht-2 ... | 2001 | 11763434 |
| blastocystis hominis. | 2001 | 11788005 | |
| diagnosis of intestinal amebiasis using salivary iga antibody detection. | attempts were made to use soluble antigen extract of strain hk-9 of entamoeba histolytica to detect salivary iga antibodies in intestinal amebiasis patients by using elisa. total salivary samples of 109 individuals were divided into four groups. group i comprised 32 patients whose stools were positive only for e. histolytica cysts and/or trophozoites. group ii comprised 12 individuals whose stools were positive for e. histolytica and other intestinal parasites. group iii comprised 36 individuals ... | 2001 | 12041582 |
| ordinary and opportunistic enteropathogens associated with diarrhea in senegalese adults in relation to human immunodeficiency virus serostatus. | a survey was conducted in dakar, senegal, to identify major types and prevalences of bacteria, parasites, fungi, and rotaviruses associated with diarrhea in relation to human immunodeficiency virus (hiv) serostatus with the goal to provide guidance to physicians for case management. | 2001 | 11953215 |
| [morphological and ultrastructural observation of blastocystis hominis]. | to observe the morphology and ultrastructure of blastocystis hominis. | 2001 | 12571946 |
| [intestinal parasitic infections in 4 child day-care centers located in san miguel del padrón municipality, havana city, 1998]. | a coproparasitologic study was performed on 456 children aged 1-5 years from 4 day-care centers located in san miguel del padrón municipality during november 1998, with the aim of finding out the behaviour of the main intestinal parasites and particularly how giardia lamblia was affecting this child population. three fecal specimens were collected from each child in every other day, which were processed by coproparasitologic methods such as direct testing and ritchie's concentration technique. t ... | 2001 | 15846922 |
| [infective etiology of diarrhea in adults with hiv infection in dakar: a case-control study on 594 patients]. | a survey was conducted in dakar, senegal from may 1997 to may 1999, to identify major types and prevalences of bacteria, parasites, fungi and rotaviruses associated with diarrhea in relation to human immunodeficiency virus serostatus with the aim to provide guidance to physicians for case management. etiologic agents were identified in a case-control study: cases were hiv-infected patients with diarrhea (hiv+ d+) and hiv seronegative patients with diarrhea (hiv- d+); controls were hiv-infected p ... | 2001 | 15773157 |
| intestinal parasites and the environment: frequency of intestinal parasites in children of neuquén, patagonia, argentina. | the frequency of intestinal parasites (ip) in 69 children between 2 and 14 years of age was studied within the city of neuquén, patagonia, argentina in relation to socioeconomic, cultural and environmental conditions. soil type was determined and contamination with ip was investigated in 75 soil samples. frequency of ip in children was 50.7%. blastocystis hominis was the most frequent species (24.6%). simultaneous infection with 2 to 4 species was found in 47,5% of the infected children. statist ... | 2001 | 17061494 |
| intestinal parasites, including cryptosporidium species, in iraqi patients with sickle-cell anaemia. | stool samples were obtained from individuals admitted to three hospitals in basra during november 1997-may 1998. of 40 patients with sickle-cell anaemia, 25 (62.5%) had parasitic infections. in the apparently healthy comparison group, 26 of 175 individuals (14.8%) had intestinal parasitic infections, a statistically significant difference. the most common intestinal parasites isolated in the sickle-cell patients were blastocystis hominis (36%) and giardia lamblia (28%). the isolation rate of cry ... | 2002 | 15339123 |
| intestinal parasitic infections in bekasi district, west java, indonesia and a comparison of the infection rates determined by different techniques for fecal examination. | this study was undertaken to determine the current status of intestinal parasitic infections among schoolchildren in west java, indonesia, and to compare the infection rates obtained by three different methods of fecal examination. a total of 285 fecal samples were collected from 131 males and 154 females at a junior high school. samples were brought to the department of parasitology, faculty of medicine, university of indonesia, and were examined for parasites by the kato-katz thick smear metho ... | 2002 | 12693577 |
| in-vitro cultivation: a sensitive method for detecting blastocystis hominis. | currently, the detection of human infection with blastocystis hominis is usually based on the examination under a light microscope of faecal samples, either directly, as 'simple smears', or after some form of concentration. whether short-term, in-vitro cultivation would increase the sensitivity of such detection remains a matter of controversy. over 900 fresh stool specimens, from soldiers in the royal thai army, were each checked for the parasite using three methods: simple smears; formalin-eth ... | 2002 | 12625935 |
| [50-year-old patient returning from hawaii with changed stool habits. blastocystis hominis]. | 2002 | 12085551 | |
| methods for cultivation of luminal parasitic protists of clinical importance. | cultivation of luminal protistan parasites has a long history. in this review we discuss the methods and media that are most widely used for the establishment and maintenance of the following organisms in culture: entamoeba histolytica, giardia intestinalis, trichomonas vaginalis, dientamoeba fragilis, blastocystis hominis, and balantidium coli. while cultivation is of limited importance in the diagnostic laboratory, it is essential to most research laboratories, and it is toward the latter that ... | 2002 | 12097242 |
| in vitro effect of nitazoxanide against entamoeba histolytica, giardia intestinalis and trichomonas vaginalis trophozoites. | nitazoxanide, a 5-nitrothiazolyl derivative, is effective in the treatment of a broad range of parasitic infections. in vitro, it is active against several protozoa, including cryptosporidium parvum, blastocystis hominis, and giardia intestinalis. the objective of this study was to determine the in vitro effect of nitazoxanide on the growth and morphology of three anaerobic protozoa (entamoeba histolytica, giardia intestinalis, and trichomonas vaginalis) and to compare these effects with those o ... | 2002 | 12120985 |
| [blastocystis hominis and blastocystosis (zierdt-garavelli disease)]. | 2002 | 12154808 | |
| prevalence of blastocystis hominis carriage in thai army personnel based in chonburi, thailand. | blastocystis hominis is one of the most common intestinal parasites found in humans. the prevalence of b. hominis carriage in personnel who worked in the first army support command, chonburi, thailand, was determined. forty-four percent of 616 stool samples were positive for b. hominis using both simple smear and concentration (formalin/ethyl acetate) techniques. the usefulness of the concentration technique in the diagnosis of b. hominis has been confirmed. of 272 positive specimens, 75% were d ... | 2002 | 12188234 |
| [infections and diseases after travelling]. | with intensifying international travel numbers of travel associated infections and diseases will increase. systematic studies on infections and diseases with regard to the travel destination in tropical and subtropical areas are scarce in germany. | 2002 | 12192633 |
| prevalence of intestinal parasitic pathogens in hiv-seropositive individuals in northern india. | to assess the prevalence of intestinal parasitic infections in human immunodeficiency virus (hiv)-seropositive subjects, fecal samples were collected from 120 hiv-seropositive patients and were analyzed for various intestinal parasites. thirty-six patients (30%) were found to harbor an intestinal parasite. cryptosporidium parvum was the most common (10.8%), followed by giardia lamblia (8.3%). cyclospora cayetanensis and blastocystis hominis each were detected in 3.3% of the patients, while isosp ... | 2002 | 12195048 |
| [utility of studying feces for the diagnosis and management of infants and preschool children with acute diarrhea]. | to analyze the results of a stool work-up protocol in a series of infants and preschoolers with acute diarrhea. | 2002 | 12216520 |
| seasonal prevalence of intestinal parasites in the united states during 2000. | one-third of 5,792 fecal specimens from 2,896 patients in 48 states and the district of columbia tested positive for intestinal parasites during the year 2000. multiple infections with 2-4 parasitic species constituted 10% of 916 infected cases. blastocystis hominis infected 662 patients (23% or 72% of the 916 cases). its prevalence appears to be increasing in recent years. eighteen other species of intestinal parasites were identified. cryptosporidium parvum and entamoeba histolytica/e. dispar ... | 2002 | 12224595 |
| evaluation of an immunochromatographic dip-strip test for the detection of cryptosporidium oocysts in stool specimens. | the study presented here examined the efficacy of a commercially available qualitative immunochromatographic assay for detecting cryptosporidium oocysts in stool samples. a total of 75 samples were tested, including 50 positive for cryptosporidium spp. by acid-fast stain, 20 positive for other parasites ( blastocystis hominis, endolimax nana, entamoeba coli, giardia lamblia, ascaris lumbricoides, strongyloides stercoralis and trichuris trichiura), and five negative samples. the observed sensitiv ... | 2002 | 12226697 |
| a cross-sectional study of intestinal parasitic infections among schoolchildren in nan province, northern thailand. | a cross-sectional study of the prevalence of intestinal parasitic infections at eight schools in bo klau district and four schools in chalerm prakiet district, nan province, in january and february, 2001. a total of 1,010 fecal samples were examined using the formalin-ether sedimentation technique. results revealed that the rate of helminthic infection was 60.0%, while protozoa accounted for 36.2% of infections; mixed infections were common, resulting in a total prevalence of both parasites of 6 ... | 2002 | 12236415 |
| incidence of blastocystis hominis in faecal samples submitted for routine microbiological analysis. | over a one-year period, 1390 faecal samples were submitted to aberystwyth public health laboratory for routine microbiological examination. all were stained using a commercial trichrome method. blastocystis hominis was detected in 96 (6.9%), making it the most common parasite found in the study. of the b. hominis-positive specimens, 73% were missed on direct microscopy. molecular typing of b. hominis has revealed extensive genetic diversity in morphologically identical strains and thus detection ... | 2002 | 12371057 |
| epidemiological survey of blastocystis hominis in huainan city, anhui province, china. | to provide scientific evidence for prevention and controlling of blastocystosis, the infection of blastocystis homonis and to study its clinical significance in huainan city, anhui province, china. | 2002 | 12378644 |
| enteropathogens associated with acute diarrhea in community and hospital patients in jakarta, indonesia. | the prevalence of bacteria, parasite and viral pathogens in 3875 patients with diarrhea in community and hospital settings from march 1997 through august 1999 in jakarta, indonesia was determined using routine bacteriology and molecular assay techniques. bacterial pathogens isolated from hospital patients were, in decreasing frequency, vibrio cholerae o1, shigella flexneri, salmonella spp. and campylobacter jejuni, while s. flexneri, v. cholerae o1, salmonella spp. and c. jejuni were isolated fr ... | 2002 | 12381465 |
| [distribution of entero-parasitic infections in the peruvian highland: study carried out in six rural communities of the department of puno, peru]. | a prevalence study was carried out in six rural communities in the peruvian highlands with the purpose of achieving a better understanding of the distribution of entero-parasites. the communities were located along the banks of lake titicaca, in the provinces of puno and el collao, department of puno, peru, at 3,800 m.a.s.l. to that effect, a total of 91 feces samples of adults and children from the following communities were analyzed: conchaca, puñutani, capalla, culta, maraesqueña, and jaillih ... | 2002 | 12525846 |
| [blastocytosis. an italian story]. | 2002 | 12532568 | |
| blastocystis hominis and blastocystosis (zierdt-garavelli disease). | 2002 | 11975117 | |
| palmoplantar pruritus subsiding after blastocystis hominis eradication. | 2002 | 12013204 | |
| a survey of blastocystis sp. in livestock, pets, and zoo animals in japan. | the prevalence of blastocystis sp. was examined in fecal samples collected from cattle, pigs, dogs, and a variety of zoo animals (primates, carnivores, herbivores, pheasants, and ducks) by direct observation of fresh fecal suspensions or cultured materials, using light microscopy. the cattle and pigs were randomly sampled from 11 and 12 commercial farms, respectively, located in the western region of japan. the dog material used in this study was obtained from pets housed in an animal shelter in ... | 2002 | 12062509 |
| recent advances in blastocystis hominis research: hot spots in terra incognita. | despite being discovered more than 80 years ago, progress in blastocystis research has been gradual and challenging, due to the small number of laboratories currently working on this protozoan parasite. to date, the morphology of blastocystis hominis has been extensively studied by light and electron microscopy but all other aspects of its biology remain little explored areas. however, the availability of numerous and varied molecular tools and their application to the study of blastocystis has ... | 2002 | 12062550 |
| hypersensitivity to non-steroidal anti-inflammatory drugs and chronic urticaria cured by treatment of blastocystis hominis infection. | 2002 | 12072100 | |
| phylogenetic position of blastocystis hominis and of stramenopiles inferred from multiple molecular sequence data. | blastocystis hominis, a parasite of the human intestine, has recently been positioned within stramenopiles by the small subunit rrna phylogeny. to further confirm its phylogenetic position using multiple molecular sequence data, we determined the nucleotide sequences putatively encoding small subunit ribosomal rna, cytosolic-type 70-kda heat shock protein, translation elongation factor 2, and the non-catalytic 'b' subunit of vacuolar atpase of b. hominis (he87-1 strain). moreover, we determined ... | 2002 | 11908898 |