Publications

multiple reproductive processes in blastocystis hominis.		2003	12855375
[intestinal parasitosis: our series from 1992 to 1995].	from 1992 to 1995 in our department 2236 researches of intestinal parasites (2194 on stool specimens, 34 on scotch tests, 8 on enterotests) were carried out on 1200 patients (703 hiv-ab negative and 497 hiv-ab positive). on the whole 387 samples (17.34%) of 203 subjects (16.92%) were found parasitized; 92 (13.08%) patients of them were hiv-ab negative and 111 (22.33%) were hiv-ab positive. we found more frequently blastocystis hominis and giardia intestinalis among hiv-ab negative subjects and c ...	1996	12858028
epidemiology and control of intestinal parasites with nitazoxanide in children in mexico.	the purpose of this study was to evaluate the efficacy and the tolerance of nitazoxanide in children as a single broad-spectrum antiparasitic agent in the treatment of mixed parasite infections with both intestinal protozoa and helminths. two hundred seventy-two children (age range = 2-14 years) participated in this study. we systematically surveyed every household head using questionnaires designed to obtain information about household socioeconomic status and hygiene. parasitic infections were ...	2003	12875284
prevalence of intestinal parasites among individuals with allergic skin diseases.	the prevalence of intestinal protozoans and helminths in stool samples of individuals with allergic cutaneous symptoms was evaluated to study a possible link between parasites and allergy. altogether, 218 patients who had chronic urticaria, atopic dermatitis, or pruritus of unknown origin were included in the study. standard laboratory tests for the detection of allergic etiology were performed for all patients. the presence of intestinal parasites was investigated using microscopy, immunofluore ...	2003	12880246
[study on morphology of blastocystis hominis in culture and from diarrhea patients].	to study morphology of different stage blastocystis hominis (b. h) for establishing a base in the research of life cycle and pathogenicity of b. h and providing information for clinical laboratory.	2003	12884629
[influence of the protozoa blastocystis hominis on hepatic function].		2003	12886585
[blastocystosis: nitazoxanide as a new therapeutic option].	we report a case of a patient with aids and severe immunosuppression who developed diarrhea due to a vacuolar stage of blastocystis hominis. the patient was treated with nitazoxanide, a 5-nitrothiazole broad-spectrum antiparasitic agent. we obtained complete remission of symptoms and eradication of the parasite. nitazoxanide is a new treatment option for blastocystosis in aids patients. further studies to test its efficacy are needed.	2003	12908045
intestinal parasitic infestations among children in an orphanage in pathum thani province.	infection caused by intestinal parasites is still a common health problem especially in children from developing countries. orphans are a group of underprivileged population in society. to evaluate the intestinal parasitic infections in children in an orphanage in pathum thani province, thailand, stool samples were collected during a cross-sectional study in april 2001. examination for intestinal parasites were performed by using simple smear, formalin-ether concentration, boeck and drbohlav's l ...	2003	12929999
[blastocystosis: controversy and indefinedness].	blastocystosis is the infection caused by blastocystis hominis. it is associated with frequent and unquestionably very important controversy and lack of definition, above all due to its implications for general assistance and medical care. in that connection, there is considerable disagreement on the subject of the pathogenicity of this protozoan, which should be categorically defined. other aspects besides the above, require clarification through results from well conducted studies aiming at at ...	2003	12937731
clinical characteristics and endoscopic findings associated with blastocystis hominis in healthy adults.	ninety-nine individuals with stools positive for blastocystis hominis but negative for other parasites were identified from medical records of healthy adults who had received a physical examination at taipei veterans general hospital from november 2000 to october 2002. the medical records of these 99 positive cases and 193 randomly selected controls, matched for age, sex, and date of examination, were retrospectively reviewed. the pathogenicity of b. hominis could not be demonstrated due to a la ...	2003	13677378
actidione on the growth of entamoeba histolytica and blastocystis hominis in vitro.		1963	14068656
[prevalence of microsporidia and other intestinal parasites in patients with hiv infection, bogota, 2001].	opportunistic intestinal parasites are a common cause of diarrhea in hiv-infected patients. to determine the prevalence of microsporidia and other opportunistic parasites infecting hiv patients in bogotá, colombia, 115 patients were examined for these infections during the year 2001. the institution and the sample percent from each are as follows: santa clara hospital, 33.0%; san pedro claver, 20.0%; simón bolívar hospital, 14.8%; san josé hospital, 13.9%; central de la policía hospital, 6.1%; c ...	2003	14582330
changing patterns in intestinal parasitic infections among southeast asian laborers in taiwan.	in order to determine whether there are changing patterns in intestinal parasitic infections among foreign laborers in north taiwan, one fecal specimen was obtained from each of 1,569 laborers and examined by the formalin-ethyl acetate sedimentation technique. the prevalence of intestinal parasitic infections was 8.2%. females (11.7%) had a significantly higher prevalence than males (6.9%), although there was no significant difference between filipinos (7.7%), thais (8.3%) and indonesians (12.5% ...	2004	14586642
polymerase chain reaction-based genotype classification among human blastocystis hominis populations isolated from different countries.	since the genotype of human blastocystis hominis isolates is highly polymorphic, pcr-based genotype classification using known sequenced-tagged site (sts) primers would allow the identification or classification of different genotypes. five populations of human b. hominis isolates obtained from japan, pakistan, bangladesh, germany, and thailand were subjected to genotype analysis by using seven kinds of sts primers. ninety-nine out of 102 isolates were identified as one of the known genotypes, w ...	2004	14598169
blastocystis hominis--an emerging cause of diarrhoea in renal transplant recipients.	blastocystis hominis is an intestinal protozoan that is emerging as an important cause of diarrhea in the immunosuppressed population. we report two cases of diarrhea caused by this organism in renal transplant recipients. the infection was diagnosed promptly by careful stool examination and treated successfully with metronidazole. these case report highlights the fact that unusual parasites like blastocystis hominis should be looked for and treated in cases of diarrhea occurring in renal transp ...	2003	14621046
intestinal parasites of the pacific.	information about intestinal parasites in hawaii and the pacific is not current. we reviewed reports on fecal samples obtained from two laboratories and found recovery rates of 9.3% in hawaii, 14.2% in saipan, 18% in rota and 9.5% in guam. the most frequently identified parasites were blastocystis hominis (7.6%), giardia lamblia (1.2%), and entamoeba coli (0.7%). although the incidence and types of organisms have changed with time, physicians in hawaii should continue looking for intestinal para ...	2003	14631593
metronidazole induces programmed cell death in the protozoan parasite blastocystis hominis.	previous studies by the authors have shown that the protozoan parasite blastocystis hominis succumbed to a cytotoxic monoclonal antibody with a number of cellular and biochemical features characteristic of apoptosis in higher eukaryotes. the present study reports that apoptosis-like features are also observed in growing cultures of axenic b. hominis upon exposure to metronidazole, a drug commonly used for the treatment of blastocystosis. upon treatment with the drug, b. hominis cells displayed k ...	2004	14702395
cryopreservation of protozoan parasites.	conventional methods for the propagation and preservation of parasites in vivo or in vitro have some limitations, including the need for labor, initial isolation and loss of strains, bacterial, and fungal contamination, and changes in the original biological and metabolic characteristics. all these disadvantages are considerably reduced by cryopreservation. in this study, we examined the effects of various freezing conditions on the survival of several protozoan parasites after cryopreservation. ...	2004	14969677
prevalence of enteric parasites in pet macaques in sulawesi, indonesia.	on the indonesian island of sulawesi, nonhuman primate pets come into frequent contact with humans, presenting the possibility of zoonotic and anthropozoonotic disease transmission. we collected fecal samples from 88 pet macaques representing six of the seven macaque species currently recognized as endemic to sulawesi (macaca nigra, m. nigrescens, m. hecki, m. tonkeana, m. maura, and m. ochreata) as well as two non-endemic species (m. fascicularis and m. nemestrina) in order to determine the pre ...	2004	14983465
[prevalence of blastocystis hominis among food handlers from caroni municipality, bolivar state, venezuela].	a cross-sectional survey was conducted to determine the prevalence of blastocystis hominis infection in a random sample of apparently healthy food handlers. a total of 415 individuals attending the manoa urban outpatient clinic (caroní municipality, bolívar state, venezuela) in the adult hygiene program and who requested health certification to work as food handlers were studied. stool samples obtained by spontaneous evacuation were examined by direct microscopy and the willis concentration meth ...	2004	14999338
molecular and phylogenetic analysis of blastocystis isolates from various hosts.	blastocystis hominis is a protozoan parasite found in humans. b. hominis-like organisms have been found in a variety of animals, but have been called blastocystis sp. because the isolates from animals were indistinguishable from b. hominis morphologically. recent molecular studies show that some isolates from animals have genetic similarity with b. hominis. however, it has been unclear whether the isolates from animals have zoonotic potential or not. in the present study, the ssurdna of 19 blast ...	2004	15041098
serum zinc and magnesium levels in patients with blastocystosis.	the aim of the study was to investigate the total content of the essential elements of zinc and magnesium levels in patients infected with blastocystis hominis. zinc and magnesium concentrations were measured in 52 patients who were positive for the intestinal parasite blastocystis hominis. scores were obtained for the positives and their age- and sex-matched 60 blastocystis hominis-negative healthy controls. for comparison of two groups of continuous variables, the independent samples t-test wa ...	2004	15051897
zoonotic intestinal parasites in papio anubis (baboon) and cercopithecus aethiops (vervet) from four localities in ethiopia.	a total of 59 faecal samples from ranging papio anubis (baboons) and another 41 from cercopithecus aethiops (vervet) from the rift valley areas of ethiopia were microscopically examined to determine the prevalence and species of major gastro-intestinal parasites of zoonotic importance. faecal smears were prepared from fresh faecal samples, stained using modified ziehl-neelsen method and microscopically examined. about 3 gm of the dropping was also preserved separately in clean and properly label ...	2004	15099809
irritable bowel syndrome: in search of an etiology: role of blastocystis hominis.	this study was designed to examine stool specimens of irritable bowel syndrome (ibs) patients for blastocystis hominis, a common intestinal parasite. one hundred fifty patients were enrolled, 95 ibs cases and 55 controls. these patients provided a medical history, and underwent physical and laboratory evaluations that included stool microscopy and culture for b. hominis and colonoscopy. the 95 cases (51 males and 44 females) had a mean +/- sd age of 37.8 +/- 13.2 years. stool microscopy was posi ...	2004	15100450
blastocystis hominis as a cause of hypoalbuminemia and anasarca.	the protozoan blastocystis hominis has been considered nonpathogenic, but this classification has come under scrutiny in light of reports in the medical literature indicating it could be the cause of intestinal disorders and, in one case, hypoalbuminemia. reported here is a severe case of infection with b. hominis that caused acute gastroenteritis with prolonged diarrhea, hypoalbuminemia and anasarca. the diagnosis was based on the parasitological finding, since no other pathological evidence wa ...	2004	15112065
polypeptides associated with in vitro cyst formation of blastocystis hominis.	the objective of this study was to characterize the polypeptides associated with cysts of blastocystis hominis. this form is believed to be infective and plays a role in parasite resistance to anti-b. hominis drugs currently used for treatment of blastocystis associated diarrhea. cysts were induced through in vitro culture of the parasite in complete medium supplemented with bacterial extract with trypticase, metronidazole or doxycycline. sds-page analysis showed almost similar polypeptide patte ...	2003	15115079
pcr-based identification of zoonotic isolates of blastocystis from mammals and birds.	the genotype of blastocystis isolated from humans and animals is highly polymorphic. therefore, it is important to compare the genotypes of blastocystis isolates from humans and animals to determine the zoonotic potential of animal isolates. pcr-based genotype classification using known sequence-tagged site (sts) primers allows identification of zoonotic isolates of animal origin. to this end, 51 isolates from monkeys, cattle, pigs, chickens, quails and pheasants were subjected to genotype analy ...	2004	15133074
comparison of methods for detecting blastocystis hominis.	in order to determine the comparative sensitivity of two methods of detecting blastocystis hominis and to investigate the seasonality of infection with this enteric protozoan parasite, the present study was conducted. in each of two 3-month periods representing winter, spring (february-april) and summer (july-september), 500 routine stool submissions were examined for b. hominis using microscopy following either formol-ether concentration or in vitro culture using jones' medium. the organism was ...	2004	15168139
a survey of blastocystis infection in anuran and urodele amphibians.	blastocystis infection in amphibians was surveyed in three species of anuran and one species of urodele amphibians captured at two distinct locations in japan. all three species of frogs were highly infected with blastocystis, while 69 individual urodele newts, cynopus pyrrhogaster, were negative for infection. eleven blastocystis isolates (47.8%) were recovered from 23 rana nigromaculata leopard frogs. twenty-three (92%) of 25 rana catesbeiana bullfrogs and all (100%) of 24 bufo japonicus japon ...	2004	15177714
isolation and characterization of the mitochondrion-like organelle from blastocystis hominis.	blastocystis hominis in an unusual protozoan parasite of the human intestinal tract. previous studies have described the presence of mitochondrial-like structures despite the anaerobic nature of the organism. in this study, we describe a simple and rapid technique to isolate and characterize mitochondrion-like organelles (mlo) from b. hominis. the parasite was disrupted using glass beads and the mlo were collected and purified using a sucrose gradient. negative staining and transmission electron ...	2004	15177908
infection status of intestinal parasites in children living in residential institutions in metro manila, the philippines.	a small scale survey was performed to know the infection status of intestinal parasite in children of the residential institutions and street communities in metro manila, philippines. a total of 284 stool samples from 11 institutions and 3 street communities was examined by the formalin-ether concentration method. the scotch tape anal swab was adapted to 121 children to investigate the infection status of enterovius vermicularis. it was found out that 62.0% of the children examined were positive ...	2004	15181346
evidence of waterborne transmission of blastocystis hominis.	a cross-sectional study was performed in february 2001 to evaluate the prevalence and risk factors of blastocystis hominis infection in army personnel who resided in an army base in chonburi, thailand. a total of 904 army personnel were enrolled in this study. short-term in vitro cultivation was used to detect b. hominis in stool samples. in this population, b. hominis was the parasite most frequently found, and was identified in 334 of 904 stool specimens (36.9%). a significant association betw ...	2004	15211009
enteropathogens associated with cases of gastroenteritis in a rural population in jordan.	stool specimens were collected from 180 patients belonging to a population of recently settled bedouins in jordan who presented with acute or persistent diarrhoea and other symptoms, and from 100 non-diarrhoeal controls. all samples were examined for parasites and bacterial pathogens by culture and pcr. bacterial isolates were tested for their susceptibility to common antimicrobial agents. pathogens and potential enteropathogens were identified from 140 (77.8%) of the patients, with more than on ...	2004	15214876
protozoal infections in the developmentally disabled: often overlooked cause of rectal digging and fecal smearing.		2004	15226486
the usefulness of short-term in vitro cultivation for the detection and molecular study of blastocystis hominis in stool specimens.	when in vitro cultivation was used as the 'gold standard' for the detection of blastocystis hominis in stool specimens, simple smear and trichrome staining showed sensitivities of 16.7% and 40.2% and specificities of 94% and 80.4%, respectively. in vitro cultivation also enhanced pcr amplification for the detection of b. hominis in stool specimens. our data show the usefulness of in vitro cultivation for the detection and molecular study of b. hominis in stool specimens.	2004	15243800
in vitro susceptibility of blastocystis hominis isolated from patients with irritable bowel syndrome.	this study aims to determine the growth pattern and in vitro susceptibility of clinical isolates of blastocystis hominis to different concentrations of metronidazole, furazolidone and ciprofloxacin. stool specimens from 25 consecutive patients with irritable bowel syndrome presenting to the gastroenterology department of aga khan university hospital between january and may 2003 are examined by microscopy and cultured for b. hominis. drug susceptibility assays are performed for metronidazole, fur ...	2004	15250669
enteric infection in relapse of inflammatory bowel disease: importance of microbiological examination of stool.	previous reports have suggested that diarrhoeal relapses of inflammatory bowel disease (ibd) may be associated or confused with enteric infection, and that treatment of such infections with appropriate antibiotics may be beneficial. to re-evaluate the suggestion that enteric infection is rare and microbiological testing of stool not routinely necessary in patients presenting with relapse of ibd, we have reviewed the incidence of concurrent infection in patients presenting in relapse over a recen ...	2004	15256979
point prevalence of cryptosporidium, cyclospora, and isospora infections in patients being evaluated for diarrhea.	from march to september 2001, 315 specimens from "nonrepeat" patients that were submitted for ova and parasite examination were stained using the kinyoun modified acid-fast stain to detect the intestinal coccidians. four patients (1.3%) were infected with coccidians, 2 with cryptosporidium parvum and 2 with cyclospora cayetanensis. no infections with isospora belli were detected. in comparison, 15 patients (4.8%) had infections with one or more intestinal parasites detected by routine trichrome ...	2004	15272527
[investigation of intestinal parasites in aids patients].	in this study, enteric parasites were investigated in the stool samples of 38 aids patients (23 with chronic diarrhea and 15 without diarrhea) prospectively. at least three stool samples from each patient were investigated microscopically for ova or trophozoites. the samples were concentrated with formol-ether method and wet preparations stained with lugol were examined. in addition, the concentrated samples were stained with modified asid-fast (kinyoun's), rhodamine-auramine, modified trichrom ...	2004	15293911
[blastocystosis: a high proportion of cases found in schoolchildren of são paulo, state of são paulo, brazil].	as a part of medical assistance activities, parasitological examination of fecal samples from 227 school children from a public institution of são paulo (sp) revealed a rather high proportion of results positive for blastocystis hominis. other protozoan and worm species were markedly scarcer, a peculiar situation according to our judgement. it is acknowledged that blastocystosis is still largely an indefinite and controversial subject, which deserves adequate analysis to avoid drawbacks in the s ...	2004	15334272
cutaneous lesions in blastocystis hominis infection.		2004	15339085
intestinal parasites, including cryptosporidium species, in iraqi patients with sickle-cell anaemia.	stool samples were obtained from individuals admitted to three hospitals in basra during november 1997-may 1998. of 40 patients with sickle-cell anaemia, 25 (62.5%) had parasitic infections. in the apparently healthy comparison group, 26 of 175 individuals (14.8%) had intestinal parasitic infections, a statistically significant difference. the most common intestinal parasites isolated in the sickle-cell patients were blastocystis hominis (36%) and giardia lamblia (28%). the isolation rate of cry ...	2002	15339123
[treatment of blastocystis hominis protozoosis with baitouweng decoction: a report of two cases].		2003	15339549
chronic urticaria and blastocystis hominis infection: a case report.	we report a case of a 45 year old woman which fulfilled the criteria of chronic urticaria (remitting and relapsing bouts of erythematous and pruriginuos lesions without angioedema, lasted four months). cutaneous manifestations were not related to a specific inducing factor, had no benefit from antihystamine and steroid drugs and were associated sometimes with mild gastroentric disorders. patient was submitted to extensive clinical, laboratory and intrumental investigations which permit to exclud ...	2004	15368795
epidemiological survey of giardia spp. and blastocystis hominis in an argentinian rural community.	the aim of this study was to relate personal data, socio-cultural and environmental characteristics, and the presence of symptoms/signs with the frequencies of giardia spp. and blastocystis hominis among a rural population in buenos aires province, argentina. of the surveyed population (350), 3.7% were infected with only giardia spp. or 22.9% with b. hominis, and 2.3% were infected with both protozoa. the frequency of infection according to sex; 6.1% of males were infected and 1.6% of females by ...	2004	15381860
[digestive parasitism in sousse area. results of a 16 years retrospective study].	to report the prevalence and type of intestinal parasites together with their kinetics in sousse region. our retrospective study concern data from the examination of 56,268 stool samples and 7552 tape tests, performed in the laboratory of parasitology of f. hached sousse hospital from 1st january 1987 up to 31 december 2002. we observed an overall parasite prevalence of 31.5%. most common parasites were protozoa, giardia lamblia, dientamoeba fragilis and blastocystis hominis being the most frequ ...	2004	15453028
blastocystis hominis, a long-misunderstood intestinal parasite.	for over 50 years, blastocystis hominis has been held to be a harmless intestinal yeast-probably frequent in stool samples from man and other primates, but usually ignored except as a possible source of confusion with entamoeba histolytica. more recently, its status as a protozoan parasite has been accepted, and it is now increasingly recognized as an agent of intestinal disease - usually self-limiting but occasionally fatal in monkeys. here, charles zierdt reviews the status o f this intriguing ...	1988	15462991
taxonomic status of blastocystis hominis.		1993	15463653
taxonomic status of blastocystis hominis: reply.		1993	15463657
the current status of blastocystis hominis.		1993	15463770
fecal-oral transmission of the cyst form of blastocystis hominis in rats.	the infectivity of two blastocystis hominis strains, rn94-9 and nih:1295:1, was examined in 3-week-old spf wistar rats. the nih:1295:1 strain, originally isolated from a guinea pig, was only able to infect rats via intracecal inoculation of the cultured organisms, while the rn94-9 strain, originally isolated from a laboratory rat, was able to infect rats by oral inoculation of the cultures due to the presence of a cystic form in the in vitro culture. since many cysts were discharged in the feces ...	2004	15480786
caspase-3-like protease influences but is not essential for dna fragmentation in blastocystis undergoing apoptosis.	blastocystis hominis undergo apoptosis after treatment with a cytotoxic monoclonal antibody (mab), 1d5, by mechanisms that are not fully understood, although our previous study demonstrated that caspase-3-like protease activity is involved. to elucidate the mechanism of mab 1d5-induced apoptosis, we inhibited blastocystis caspase-3-like protease to investigate if there would be a concomitant decrease in in situ dna fragmentation. however, mab 1d5-induced apoptosis, evidenced by dna fragmentation ...	2004	15540464
surveillance for enteric parasites among u.s. military personnel and civilian staff on joint task force base-bravo in soto cano, honduras and the local population in comayagua and la paz, honduras.	a study was conducted in honduras to address whether military personnel assigned to joint task force-bravo in soto cano, honduras, routinely acquire parasite infections, and the results were compared with those collected from civilian base workers and the general local population in the nearby towns of comayagua and la paz. results from this study report 21 species of enteric parasites among hondurans living in comayagua and la paz, 13 species among local hondurans working as base civilian perso ...	2004	15605940
prevalence of intestinal parasitic pathogens among hiv-positive individuals in iran.	parasites are important enteric pathogens among patients with human immunodeficiency virus (hiv) infection. there have been very few reports on the prevalence of intestinal parasites among such patients in iran. to determine the prevalence of intestinal parasites among hiv-positive individuals, we collected single stool samples and analyzed them for detection of various intestinal parasites from 206 hiv-positive individuals with different immune status visited in different medical centers in ira ...	2004	15623953
disparities in parasitic infections, perceived ill health and access to health care among poorer and less poor schoolchildren of rural côte d'ivoire.	differences in the state of health between rural and urban populations living in africa have been described, yet only few studies analysed inequities within poor rural communities. we investigated disparities in parasitic infections, perceived ill health and access to formal health services among more than 4000 schoolchildren from 57 primary schools in a rural area of western côte d'ivoire, as measured by their socioeconomic status. in a first step, we carried out a cross-sectional parasitologic ...	2005	15655013
blastocystis hominis infection in irritable bowel syndrome patients.	irritable bowel syndrome (ibs) is a functional bowel disorder in which abdominal pain is associated with a defect or a change in bowel habits. subtle inflammation, especially after infectious enteritis, has been sometimes suspected as one mechanism of pathogenesis. this research was performed (1) to evaluate the prevalence of parasitic infections and (2) the possible association of ibs and parasitic infections. fifty-nine ibs patients were recruited using symptom-based criteria (rome criteria ii ...	2004	15689092
[blastocystosis is human protozoosis].		2004	15689141
etiology of diarrhea in pediatric outpatient settings.	the frequency with which bacteria cause diarrhea evaluated in ambulatory settings is often unknown. we attempted to determine the microbiologic etiology of diarrhea in a private pediatric practice (site a) and a clinic serving largely immigrant children (site b) and to establish guidelines for bacterial culture.	2005	15702043
intestinal parasites in children from three west texas border communities.		2004	15724569
the effects of extracts from anti-diarrheic thai medicinal plants on the in vitro growth of the intestinal protozoa parasite: blastocystis hominis.	the activities of n-hexane, dichloromethane and methanol extracts from five anti-diarrheic thai medicinal plants, acacia catechu (fabaceae) resin, amaranthus spinosus (amaranthaceae) whole plant, brucea javanica (simaroubaceae) seed, piper longum (piperaceae) fruit and quercus infectoria (fagaceae) nut gall were tested against the in vitro growth of fresh isolates of the intestinal protozoan parasite, blastocystis hominis. the extracts at concentrations that ranged from 62.5 to 2000 microg/ml, w ...	2005	15763365
[infective etiology of diarrhea in adults with hiv infection in dakar: a case-control study on 594 patients].	a survey was conducted in dakar, senegal from may 1997 to may 1999, to identify major types and prevalences of bacteria, parasites, fungi and rotaviruses associated with diarrhea in relation to human immunodeficiency virus serostatus with the aim to provide guidance to physicians for case management. etiologic agents were identified in a case-control study: cases were hiv-infected patients with diarrhea (hiv+ d+) and hiv seronegative patients with diarrhea (hiv- d+); controls were hiv-infected p ...	2001	15773157
parasitic zoonoses in papua new guinea.	relatively few species of zoonotic parasites have been recorded in humans in papua new guinea. a greater number of potentially zoonotic species, mostly nematodes, occur in animals but are yet to be reported from humans. protozoa is the best represented group of those infecting man, with giardia duodenalis, cryptosporidium parvum, cyclospora cayetanesis, toxoplasma gondii, sarcocystis spp., entamoeba polecki, balantidium coli and, possibly, blastocystis hominis. the only zoonotic helminths infect ...	2005	15831107
[intestinal parasitic infections in 4 child day-care centers located in san miguel del padrón municipality, havana city, 1998].	a coproparasitologic study was performed on 456 children aged 1-5 years from 4 day-care centers located in san miguel del padrón municipality during november 1998, with the aim of finding out the behaviour of the main intestinal parasites and particularly how giardia lamblia was affecting this child population. three fecal specimens were collected from each child in every other day, which were processed by coproparasitologic methods such as direct testing and ritchie's concentration technique. t ...	2001	15846922
molecular characterization of blastocystis isolates in the philippines by riboprinting.	extensive genomic polymorphism has been demonstrated among morphologically identical blastocystis isolates. to this end, 32 blastocystis isolates from the philippines (12 from humans, 12 from pigs and 8 from chickens) were analyzed genetically by riboprinting or restriction fragment length polymorphism (rflp) analysis of polymerase chain reaction (pcr) amplified small subunit rdna. three distinct riboprint patterns were observed from the hinfi digestion, while four patterns resulted from the rsa ...	2005	15886995
programmed cell death in blastocystis hominis occurs independently of caspase and mitochondrial pathways.	we demonstrated previously that a cytotoxic monoclonal antibody (mab) 1d5 elicits a programmed cell death (pcd) response in blastocystis hominis and showed that caspase-3-like protease influences but is not essential for pcd in mab 1d5-treated b. hominis. we also showed that mitochondrial dysregulation played a role in cell death. in the current study, we further analyzed the signaling pathways involved in pcd mediated by mab 1d5. b. hominis cells were treated with mab 1d5 or control mab 5, eith ...	2005	15913879
blastocystis hominis and the evaluation of efficacy of metronidazole and trimethoprim/sulfamethoxazole.	blastocystis hominis is commonly found in the intestinal tract of humans. although the pathogenicity of this unicellular parasite is controversial, anti-protozoan agents are usually administered to infected individuals. at present, the first choice of chemotherapeutic agent is metronidazole as described in the literature. in this study, we evaluated the effects of metronidazole and trimethoprim/sulfamethoxazole (tmp/smx) on persons infected with b.hominis. a total of 104 subjects infected with b ...	2005	15915364
microorganisms and parasites in chronic infective diarrhea.	to reveal the pattern of microorganisms in chronic infective diarrhea cases.	2004	15931720
parasites in nosocomial diarrhoea: are they underestimated?	nosocomial diarrhoea, defined as diarrhoea occurring more than 72 h after hospital admission, is reported to account for <1% of endemic nosocomial infections and 17% of epidemic nosocomial infections. the yield of diagnoses from stool cultures in nosocomial diarrhoea is low, and information regarding the role of parasites is limited. we conducted a study to determine the responsible bacterial and parasitological pathogens from nosocomial diarrhoea cases in our 2000-bed tertiary care facility ove ...	2005	15949620
comparison of two methods (microscopy and enzyme-linked immunosorbent assay) for the diagnosis of amebiasis.	diagnosis of amebiasis is usually performed on a clinical basis alone in most endemic countries having limited economic resources. this epidemiological study was conducted using modern diagnostic tests for amebiasis in the southeastern region of turkey, an endemic area for amebiasis. the population of this study included patients with symptomatic diarrhea/dysentery attending both yuzuncu yil university, van and harran university, sanliurfa, turkey. a total of 380 stool specimens were collected a ...	2005	15955332
[blastocystosis: a high prevalence of cases found in patients from health center of soledad, anzoategui state, venezuela].	the parasitological examination of fecal samples from 98 patients from an ambulatory health center of soledad, anzoategui, venezuela revealed a rather high proportion of results positive for blastocystis hominis (46.9%).	2005	16082488
prevalence of blastocystis hominis among "healthy" food handlers in dammam, saudi arabia.	a prospective study was carried out to see the prevalence of b. hominis in food handlers in dammam, eastern province of saudi arabia. a total of 17073 food handlers were examined, 8.50% persons were positive for this organism in their stool. in 2.4% of the food handlers, b. hominis was the only parasite, whereas in 1.9% and 4.2% of the cases, this organism was found in association with pathogenic and non pathogenic parasites respectively. we could find the correlation between the dietary habits ...	2005	16083054
blastocystis hominis among symptomatic and asymptomatic individuals in talkha center, dakahlia governorate, egypt.	blastocystis hominis is now getting acceptance as an agent of human intestinal disease. b. hominis in stool samples of symptomatic and asymptomatic individuals was evaluated as a possible cause of gastro-intestinal troubles. b. hominis was found in 106 (10.1%) out of 1050 individuals examined from six villages and one city in talkha center, dakahlia governorate. the highest infection rate was in manshayt el-badawy village (25.47%), whereas talkha city showed the lowest rate (4.73%). age group 10 ...	2005	16083074
no correlation between clinical symptoms and blastocystis hominis in immunocompetent individuals.	abstract background and aim: previous reports regarding the clinical significance and pathogenicity of blastocystis hominis have been contradictory. the aim of this study was to examine the association between blastocystis and gastrointestinal symptoms in immunocompetent individuals. methods: we monitored over 2800 healthy people for a period of 15 months, and took stool specimens during both asymptomatic periods and during periods of gastrointestinal symptoms. results: after exclusion of indivi ...	2005	16105126
blastocystis hominis and other intestinal parasites in a community of pitanga city, paraná state, brazil.	the objective was to estimate the prevalence of blastocystis hominis, to evaluate the effectiveness of different techniques for its diagnosis as well as to estimate the prevalence of other intestinal parasites in the community of campo verde, a district of pitanga. the work was carried out from august to october 2004. samples of feces from children and adults were collected and submitted to the techniques of direct wet mount, flotation in zinc sulphate solution, tube sedimentation, sedimentation ...	2005	16138204
degradation of human secretory immunoglobulin a by blastocystis.	microbial immunoglobulin a (iga) proteases cleave human secretory iga, promoting the mucosal adhesion of pathogens. to investigate if the enteric protozoan blastocystis degrades human secretory iga, cell lysate and conditioned medium from two species were exposed to immunoglobulin a. secretory iga was cleaved by both cell lysate and conditioned medium with mainly cysteine proteinase activity in b. hominis b isolate and aspartic proteinase activity in b. ratii wr1 isolate. these findings suggest ...	2005	16151742
remission of delayed pressure urticaria after eradication of blastocystis hominis.		2005	16191864
[intestinal parasitic diseases in hiv-infected patients in uzbekistan].	intestinal parasitic diseases were diagnosed in 100 hiv-infected patients at different stages of disease (its asymptomatic form, persistent generalized lymphoadenopathy, pre-aids, and aids) (group 1), 100 tashkent residents (group 2), and 349 patients with gastrointestinal diseases, allergic dermatoses, and skin depigmentation foci (group 3). the hiv-infected patients were found to have virtually all parasites, such as giardia lamblia, cryptosporidium parvum, chilomastix mesnili, entamoeba coli, ...	2005	16212098
protozoan programmed cell death--insights from blastocystis deathstyles.	programmed cell death (pcd) is an essential process in the growth and development of multicellular organisms. however, accumulating evidence indicates that unicellular eukaryotes can also undergo pcd with apoptosis-like features. the protozoan parasite blastocystis hominis has been reported to exhibit both apoptotic and non-apoptotic features of pcd when exposed to a variety of stimuli. recent observations of pcd pathways in blastocystis suggest that this protozoan, as is the case with its multi ...	2005	16223601
effect of nitazoxanide in persistent diarrhea and enteritis associated with blastocystis hominis.	the aim of this study was to evaluate the efficacy of nitazoxanide for the treatment of diarrhea and enteritis associated with blastocystis hominis as the sole identified pathogen in children and adults from the nile delta of egypt.	2005	16234044
the association of blastocystis hominis and endolimax nana with diarrheal stools in zambian school-age children.	to determine the prevalence of endoparasites and their association with diarrhea, a survey was conducted in the southern province of zambia that used conventional and molecular techniques applied to stool and urine samples from school-age children (n = 93). almost half of the stools (49.5%) were diarrhetic. the overall prevalence of endolimax nana, schistosoma haematobium, blastocystis hominis, giardia lamblia, cryptosporidium parvum, encephalitozoon intestinalis, and strongyloides stercoralis w ...	2005	16249910
predominance of amoeboid forms of blastocystis hominis in isolates from symptomatic patients.	blastocystis hominis is one of the most common human parasites that inhabit the intestinal tract. conflicting reports continue to exist regarding the existence and the functional role of the amoeboid forms in the life cycle of the parasite. the present study investigates the presence of these forms in 20 isolates obtained from ten symptomatic and asymptomatic patients respectively. a total of 10,000 parasite cells per ml from each isolate were inoculated into three culture tubes each containing ...	2006	16323025
[cyclosporosis: an emergent parasitosis. (ii) microbiological diagnosis through a new staining technique].	describe a new staining technique for the diagnosis of cyclospora infection through lab tests. a differential microscopic diagnosis between cyclospora and blastocystis hominis is conducted.	2005	16333388
[prevalence of giardiasis and intestinal parasites in pre-school children from homes being attended as part of a state programme in armenia, colombia].	determining the prevalence of giardiasis and intestinal parasitism in pre-school children from the instituto colombiano de bienestar familiar (icbf).	2005	16396421
prevalence of blastocystis hominis infection in asymptomatic individuals from bangkok, thailand.	fresh stool examination was performed from 2,230 participants who enrolled in annual check-up programs of the faculty of medical technology, mahidol university in 1999-2000 and 2004. in this study, blastocystis hominis infection was diagnosed by culturing in jones' media. a total of 21% of fecal specimens (in 1999-2000) and 13.7% (in 2004) were positive for b. hominis. the vacuolated form was the predominant form found in culture solution after 48 hours of incubation. the distribution of infecti ...	2005	16438173
current infection rate of giardia lamblia in two provinces of thailand.	the aim of this study was to survey for the current rate of giardia lamblia infection in three different districts in two provinces of thailand, surin and samut sakhon, in march 2002, october 2003, and march 2004. two districts are rural areas and another is sub-urban. volunteers of any age from surin province and children aged between 5 to 7 years old from three secondary schools in samut sakhon were enrolled for the stool examination. the method used was stool examination by both simple smear ...	2005	16438174
protease activity of blastocystis hominis.	parasite-derived proteases are important for the parasite life cycle and the pathogenesis of the disease they produce. proteases of intestinal protozoan parasite blastocystis hominis were studied for the first time with azocasein assays and gelatin sds-page analysis. parasitic lysates were found to have high protease activity and nine protease bands of low (20-33 kda) and high (44-75 kda) molecular weights were reported. proteases were found to be ph-dependent and highest proteolytic activity wa ...	2006	16518611
[prevalence of intestinal parasites in children of yukpa ethnia in toromo, zulia state, venezuela].	yupkpa community in perijá sierra does not have drinking water or sewage elimination systems. thus it becomes a propitious place for infection with intestinal parasites.	2006	16532165
blastocystis hominis: its characteristics and its prevalance in intestinal content and feces in south carolina.		1917	16558755
[experimental infection of mice with blastocystis hominis].	to seek a better pathway and proper number of parasites for blastocystis hominis (b.h) infection in normal and immunocompromised icr mice.	2005	16566218
patients with gastrointestinal complains due to enteric parasites, with reference to entamoeba histolytica/dispar as dected by elisa e. histolytica adhesion in stool.	a total of 210 patients with gastrointestinal troubles, of both sex and a mean age of 32 +/- 6.1 years, selected from the outpatient's clinics of al-azhar university hospitals. 115 (54.76%) had dysentery, 95 (45.23%) did not have dysentery, 15 (14%) suffered flatulence, 20 (9.52%) had epi-gastric pain, 19 (9.05%) had vague abdominal pain, 5 vomiting (5.2%) and 10 (4.9%) had fever. two symptoms were in 29 (13.81%) patients and three symptoms in 12 (5.71%). of the 210 patients, 20 (9.9%) had helmi ...	2006	16605100
[epidemiologic and laboratory assessments of etiologic implications of blastocystis hominis in gastrointestinal diseases].	authors present the first laboratory and epidemiological results which reveal the circulation in a population of protozoan blastocystis hominis and its implication in the determinations of some gastrointestinal troubles, with fever, diarrhea and constipation, intense intestinal meteorism, associated with abdominal pain and cramps. out of the 3106 investigated patients, 9.7% presented b. hominis as a unique etiologic agent, with an increased prevalence in adults (74.3%) and women (65.3%). blastoc ...	2005	16607843
detection of dientamoeba fragilis and blastocystis hominis using a simple staining method.		2006	16613140
comparison of fecal microflora in children with atopic eczema/dermatitis syndrome according to ige sensitization to food.	atopic eczema/dermatitis syndrome (aeds) commonly often arises during early infancy. in several intervention studies a beneficial influence on aeds course of certain intestinal bacteria, administered as 'probiotics', has been described. to evaluate the possible role of the natural intestinal microflora in children with allergic eczema/dermatitis syndrome regarding immediate type hypersensitivity to food allergens, children with food allergy (aaeds, n = 68) have been compared with children withou ...	2006	16618364
pcr fingerprinting of blastocystis isolated from symptomatic and asymptomatic human hosts.	genomic dna from 16 blastocystis hominis isolates comprising of eight asymptomatic isolates (a1-a8) and eight symptomatic isolates (s1-s8) was amplified by arbitrarily primed polymerase chain reaction (ap-pcr) using 38 arbitrary 10-mer primers. six primers (a10, b5, c20, d1, f6, and f10) generated reproducible dna fingerprints. ap-pcr amplification revealed similar dna fingerprints among all symptomatic isolates (s1-s8) with common bands at 850 bp using primer a10, 920 bp using primer b5, and 1. ...	2006	16628457
chronic urticaria due to blastocystis hominis.	a 24-year-old woman had a 9-week history of second to third daily urticaria that began after an episode of contact urticaria to topical bufexamac. she was found to have an underlying gastrointestinal infection with blastocystis hominis. this was thought to be clinically relevant as she had a history of mild chronic diarrhoea. after treatment of the blastocystis hominis, her urticaria ceased. this could indicate the importance of performing stool microscopy and culture on all patients with chroni ...	2006	16637808
epidemiology of blastocystis hominis and other intestinal parasites in a vietnamese female immigrant population in southern taiwan.	there has been a rapid increase in cross-border female marriage immigrants in taiwan. in this study, 1,434 vietnamese female marriage immigrants arriving between july 1998 and june 2001 were examined for intestinal parasites. most (77.9%) of these women were 20-29 years of age. the prevalence of intestinal parasite infection was 37.7%, and the trend increased from 1999 to 2001 (statistically significant, p < 0.0001), but decreased among age subgroups (p < 0.0001). among the 20 species of intesti ...	2006	16679297
the prevalence of parasites in commonly used leafy vegetables in south western, saudi arabia.	to determine the degree of contamination caused by parasites in commonly used leafy vegetables in abha, kingdom of saudi arabia (ksa).	2006	16680247
genetic variability of blastocystis hominis isolates in china.	to determine if genetic diversity of blastocystis hominis exists in china, 35 b. hominis isolates obtained from 19 asymptomatic infected individuals and 16 patients with gastrointestinal symptoms were genotyped by pcr using seven pairs of known sequenced-tagged site (sts) primers. out of the 35 isolates, 29 were identified as one of the known genotypes, while five isolates showed two distinct genotypes, and one isolate was an unknown genotype as this was negative with all the sts primers. in thi ...	2006	16688468
[emphasizing of blastocystis hominis on culture media].	the aim of our study was to determine the frequency of blastocystis hominis in the stool specimens sent to the parasitological laboratory of the mureş county hospital between october 2003 and may 2004. the 124 inoculated specimens showed positive values in 41.93%. after an incubation in anaerobic conditions at 37 degrees c for 24, 72 and 96 hours in liquid and biphasic media, the last proved to be better (69,24%) at an incubation time of 72 hours.	2004	16752753
amoeboid form of blastocystis hominis - a detailed ultrastructural insight.	the amoeboid form of blastocystis hominis has been reported infrequently, and its morphological descriptions have yielded conflicting and confusing reports. in the present study, we used the amoeboid forms seen predominantly in symptomatic patients infected with blastocystis to provide detailed descriptions on the fine surface structure and intracellular morphology. scanning electron microscopy revealed the irregular shape of the amoeboid form, with an intercalated fibrillar structure and a high ...	2006	16816959