Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [respiratory virus infections in adult patients hospitalized in an internal medicine unit]. | respiratory viral infections (rvi) can be associated with a wide range of clinical manifestations. | 2014 | 25327313 |
| immunity to rsv in early-life. | respiratory syncytial virus (rsv) is the commonest cause of severe respiratory infection in infants, leading to over 3 million hospitalizations and around 66,000 deaths worldwide each year. rsv bronchiolitis predominantly strikes apparently healthy infants, with age as the principal risk factor for severe disease. the differences in the immune response to rsv in the very young are likely to be key to determining the clinical outcome of this common infection. remarkable age-related differences in ... | 2014 | 25324843 |
| reactive oxygen species at the crossroads of inflammasome and inflammation. | inflammasomes form a crucial part of the innate immune system. these are multi-protein oligomer platforms that are composed of intracellular sensors which are coupled with caspase and interleukin activating systems. nod-like receptor protein (nlrp) 3, and 6 and nlrc4 and aim2 are the prominent members of the inflammasome family. inflammasome activation leads to pyroptosis, a process of programmed cell death distinct from apoptosis through activation of caspase and further downstream targets such ... | 2014 | 25324778 |
| therapy of respiratory viral infections with intranasal sirnas. | chemically synthesized short interfering rna (sirna) has ushered a new era in the application of rna interference (rnai) against viral genes. we have paid particular attention to respiratory viruses that wreak heavy morbidity and mortality worldwide. the clinically significant ones include respiratory syncytial virus (rsv), parainfluenza virus (piv) (two paramyxoviruses), and influenza virus (an orthomyxovirus). as the infection by these viruses is clinically restricted to the respiratory tissue ... | 2015 | 25319655 |
| detection of respiratory viruses by real-time polymerase chain reaction in outpatients with acute respiratory infection. | viruses are the major contributors to the morbidity and mortality of upper and lower acute respiratory infections (aris) for all age groups. the aim of this study was to determine the frequencies for a large range of respiratory viruses using a sensitive molecular detection technique in specimens from outpatients of all ages with aris. nasopharyngeal aspirates were obtained from 162 individuals between august 2007-august 2009. twenty-three pathogenic respiratory agents, 18 respiratory viruses an ... | 2014 | 25317699 |
| the role of multiplex pcr in respiratory tract infections in children. | infants, toddlers, and children of primary-school age without any special risk factors generally have three to ten febrile respiratory infections per year. most such infections are of viral origin and self-limiting, but viral infection is often hard to distinguish from bacterial infection. the use of a multiplex polymerase chain reaction (pcr) to detect viruses in respiratory secretions is potentially beneficial, as it might help physicians avoid giving antibiotics unnecessarily. | 2014 | 25316519 |
| central nervous system alterations caused by infection with the human respiratory syncytial virus. | worldwide, the human respiratory syncytial virus (hrsv) is the leading cause of infant hospitalization because of acute respiratory tract infections, including severe bronchiolitis and pneumonia. despite intense research, to date there is neither vaccine nor treatment available to control hrsv disease burden globally. after infection, an incubation period of 3-5 days is usually followed by symptoms, such as cough and low-grade fever. however, hrsv infection can also produce a larger variety of s ... | 2014 | 25316031 |
| risk factors of prolonged hospital stay in children with viral severe acute respiratory infections. | severe acute lower respiratory infections (saris) are one of the major causes of morbidity and mortality in young children, especially in developing countries. the present study focused on detection of risk factors for prolonged hospital stays among children with viral saris. | 2014 | 25313605 |
| does cesarean section pose a risk of respiratory syncytial virus bronchiolitis in infants and children? | to determine the risk of acquiring acute respiratory syncytial virus (rsv) bronchiolitis in infants and children delivered by the mode of cesarean section (c-section). | 2014 | 25312107 |
| reprint of: perinatal and early childhood environmental factors influencing allergic asthma immunopathogenesis. | the prevalence of asthma has increased dramatically over the past several decades. while hereditary factors are highly important, the rapid rise outstrips the pace of genomic variation. great emphasis has been placed on potential modifiable early life exposures leading to childhood asthma. | 2014 | 25308874 |
| partial palivizumab prophylaxis and increased risk of hospitalization due to respiratory syncytial virus in a medicaid population: a retrospective cohort analysis. | infection with respiratory syncytial virus (rsv) is common among young children insured through medicaid in the united states. complete and timely dosing with palivizumab is associated with lower risk of rsv-related hospitalizations, but up to 60% of infants who receive palivizumab in medicaid population do not receive full prophylaxis. the purpose of this study was to evaluate the association of partial palivizumab prophylaxis with the risk of rsv hospitalization among high-risk medicaid-insure ... | 2014 | 25308481 |
| humidity and respiratory virus transmission in tropical and temperate settings. | influenza and respiratory syncytial virus (rsv) are similarly structured viruses with similar environmental survival, but different routes of transmission. while rsv is transmitted predominantly by direct and indirect contact, influenza is also transmitted by aerosol. the cold, dry conditions of temperate winters appear to encourage the transmission of both viruses, by increasing influenza virus survival in aerosols, and increasing influenza and rsv survival on surfaces. in contrast, the hot, we ... | 2015 | 25307020 |
| respiratory syncytial virus (rsv) and its propensity for causing bronchiolitis. | infants and young children with acute onset of wheezing and reduced respiratory airflows are often diagnosed with obstruction and inflammation of the small bronchiolar airways, ie bronchiolitis. the most common aetological agents causing bronchiolitis in young children are the respiratory viruses, and of the commonly encountered respiratory viruses, respiratory syncytial virus (rsv) has a propensity for causing bronchiolitis. indeed, rsv bronchiolitis remains the major reason why previously heal ... | 2015 | 25302625 |
| antibiotics for bronchiolitis in children under two years of age. | bronchiolitis is a serious, potentially life-threatening respiratory illness commonly affecting babies. it is often caused by respiratory syncytial virus (rsv). antibiotics are not recommended for bronchiolitis unless there is concern about complications such as secondary bacterial pneumonia or respiratory failure. nevertheless, they are often used. | 2014 | 25300167 |
| protection and mechanism of action of a novel human respiratory syncytial virus vaccine candidate based on the extracellular domain of small hydrophobic protein. | infections with human respiratory syncytial virus (hrsv) occur globally in all age groups and can have devastating consequences in young infants. we demonstrate that a vaccine based on the extracellular domain (she) of the small hydrophobic (sh) protein of hrsv, reduced viral replication in challenged laboratory mice and in cotton rats. we show that this suppression of viral replication can be transferred by serum and depends on a functional igg receptor compartment with a major contribution of ... | 2014 | 25298406 |
| safety and effectiveness of palivizumab in children at high risk of serious disease due to respiratory syncytial virus infection: a systematic review. | lower respiratory tract infection (lrti) is the leading cause of infant mortality globally in post-neonatal infants (i.e., 28-364 days of age). respiratory syncytial virus (rsv) is the most commonly identified pathogen for infant lrti and is the second most important cause of death in post-neonatal infants. despite 50 years of rsv vaccine research, there is still no approved vaccine. therefore, passive immunity with the monoclonal antibody palivizumab is the sole regulatory-approved option for t ... | 2014 | 25297809 |
| respiratory syncytial virus-associated hospitalizations in pre-mature infants in lima, peru. | we conducted a prospective cohort study in four hospitals in lima, peru in infants with a birth weight ≤ 1,500 g followed from birth hospital discharge up to 1 year of age to determine the incidence of respiratory syncytial virus (rsv) hospitalizations. we enrolled 222 infants from march of 2009 to march of 2010: 48 infants with a birth weight < 1,000 g and 174 infants with a birth weight of 1,000-1,500 g (birth weight = 1,197 ± 224 g; gestational age = 30.1 ± 2.6 weeks). there were 936 episodes ... | 2014 | 25294617 |
| serial intervals of respiratory infectious diseases: a systematic review and analysis. | the serial interval of an infectious disease represents the duration between symptom onset of a primary case and symptom onset of its secondary cases. a good evidence base for such values is essential, because they allow investigators to identify epidemiologic links between cases and serve as an important parameter in epidemic transmission models used to design infection control strategies. we reviewed the literature for available data sets containing serial intervals and for reported values of ... | 2014 | 25294601 |
| novel respiratory syncytial virus (rsv) genotype on1 predominates in germany during winter season 2012-13. | respiratory syncytial virus (rsv) is the leading cause of hospitalization especially in young children with respiratory tract infections (rti). patterns of circulating rsv genotypes can provide a better understanding of the molecular epidemiology of rsv infection. we retrospectively analyzed the genetic diversity of rsv infection in hospitalized children with acute rti admitted to university hospital heidelberg/germany between october 2012 and april 2013. nasopharyngeal aspirates (npa) were rout ... | 2014 | 25290155 |
| molecular evolution of human respiratory syncytial virus attachment glycoprotein (g) gene of new genotype on1 and ancestor na1. | we conducted a comprehensive genetic analysis of the c-terminal 3rd hypervariable region of the attachment glycoprotein (g) gene in human respiratory syncytial virus subgroup a (hrsv-a) genotype on1 (93 strains) and ancestor na1 (125 strains). genotype on1 contains a unique mutation of a 72 nucleotide tandem repeat insertion (corresponding to 24 amino acids) in the hypervariable region. the bayesian markov chain monte carlo (mcmc) method was used to conduct phylogenetic analysis and a time scale ... | 2014 | 25283803 |
| leukemia inhibitory factor protects the lung during respiratory syncytial viral infection. | respiratory syncytial virus (rsv) infects the lung epithelium where it stimulates the production of numerous host cytokines that are associated with disease burden and acute lung injury. characterizing the host cytokine response to rsv infection, the regulation of host cytokines and the impact of neutralizing an rsv-inducible cytokine during infection were undertaken in this study. | 2014 | 25277705 |
| visualizing the replication of respiratory syncytial virus in cells and in living mice. | respiratory syncytial virus (rsv) is the most important cause of severe lower-respiratory tract disease in calves and young children, yet no human vaccine nor efficient curative treatments are available. here we describe a recombinant human rsv reverse genetics system in which the red fluorescent protein (mcherry) or the firefly luciferase (luc) genes are inserted into the rsv genome. expression of mcherry and luc are correlated with infection rate, allowing the monitoring of rsv multiplication ... | 2014 | 25277263 |
| comparison of the imdx influenza a virus, influenza b virus, and respiratory syncytial virus a/b assay on the m2000 platform with real-time reverse transcriptase pcr assays. | 2014 | 25275003 | |
| [detection of respiratory viruses in influenza-like illness in shijiazhuang, china in 2011]. | this study aimed to investigate viral infections and the prevalence of influenza-like illness (ili) in shijiazhuang, china, in 2011 and to provide a scientific basis for the diagnosis and control of respiratory tract infections. throat swab specimens were collected from 483 cases of ili who were outpatients in the influenza surveillance sentinel hospitals in shijiazhuang between january and december 2011. all specimens were examined by multiplex rt-pcr for the following 15 respiratory tract viru ... | 2014 | 25272592 |
| an insect cell derived respiratory syncytial virus (rsv) f nanoparticle vaccine induces antigenic site ii antibodies and protects against rsv challenge in cotton rats by active and passive immunization. | post-infectious immunity to respiratory syncytial virus (rsv) infection results in limited protection as evidenced by the high rate of infant hospitalization in the face of high titer, maternally derived rsv-specific antibodies. by contrast, rsv fusion (f) glycoprotein antigenic site ii humanized monoclonal antibodies, palivizumab and motavizumab, have been shown to reduce rsv-related hospitalization in infants. immunogenicity and efficacy studies were conducted in cotton rats comparing a recomb ... | 2014 | 25269094 |
| respiratory syncytial virus: pathology, therapeutic drugs and prophylaxis. | human respiratory syncytial virus (hrsv) is the leading cause of lower respiratory tract diseases, affecting particularly newborns and young children. this virus is able to modulate the immune response, generating a pro-inflammatory environment in the airways that causes obstruction and pulmonary alterations in the infected host. to date, no vaccines are available for human use and the first vaccine that reached clinical trials produced an enhanced hrsv-associated pathology 50 years ago, resulti ... | 2014 | 25268876 |
| impaired gamma delta t cell-derived il-17a and inflammasome activation during early respiratory syncytial virus infection in infants. | respiratory syncytial virus (rsv) infection remains a significant global health burden disproportionately affecting infants and leading to long-term lung disease. interleukin (il)-17a has been shown to be involved in regulating viral and allergic lung inflammatory responses, which has led to a more recent interest in its role in rsv infection. using a neonatal mouse model of rsv, we demonstrate that neonates fail to develop il-17a responses compared with adult mice; the main immediate il-17a con ... | 2015 | 25267484 |
| the association of meningococcal disease with influenza in the united states, 1989-2009. | prior influenza infection is a risk factor for invasive meningococcal disease. quantifying the fraction of meningococcal disease attributable to influenza could improve understanding of viral-bacterial interaction and indicate additional health benefits to influenza immunization. | 2014 | 25265409 |
| diagnosing heart failure in children with congenital heart disease and respiratory syncytial virus bronchiolitis. | the objective of this study is to examine if the b-type natriuretic peptide (bnp) can be used in diagnosing heart failure (hf) in children with congenital heart disease (chd) who present to the emergency department (ed) with acute bronchiolitis. | 2014 | 25261398 |
| nasopharyngeal gene expression, a novel approach to study the course of respiratory syncytial virus infection. | respiratory syncytial virus (rsv) causes mild infections in the vast majority of children. however, in some cases, it causes severe disease, such as bronchiolitis and pneumonia. development of severe rsv infection is determined by the host response. therefore, the main aim of this study was to identify biomarkers associated with severe rsv infection. to identify biomarkers, nasopharyngeal gene expression was profiled by microarray studies, resulting in the selection of five genes: ubiquitin d, t ... | 2015 | 25261323 |
| burden of respiratory syncytial virus infection in young children. | respiratory syncytial virus (rsv) is the most frequent and important cause of lower respiratory tract infection in infants and children. it is a seasonal virus, with peak rates of infection occurring annually in the cold season in temperate climates, and in the rainy season, as temperatures fall, in tropical climates. high risk groups for severe rsv disease include infants below six mo of age, premature infants with or without chronic lung disease, infants with hemodynamically significant congen ... | 2012 | 25254161 |
| ribavirin: the need for exposure precautions. | ribavirin is used in the treatment of respiratory syncytial virus (rsv) in high-risk patients, including patients who have undergone hematopoietic stem cell transplantation, to reduce mortality from rsv pneumonia. it is classified as a hazardous drug with potential for carcinogenicity and teratogenicity. very few recent studies have examined the risk of exposure, and recommendations for exposure precautions are lacking. administration should include the use of personal protective equipment and t ... | 2014 | 25253120 |
| detection of influenza and other respiratory viruses carried out in the influenza project - monitoring vaccine effectiveness (i-move). | the project influenza vaccine effectiveness-monitoring (i-move) is part of the european research carried out by the ecdc (european center for disease prevention and control), aimed at monitoring the effectiveness of vaccination in europe during the growing incidence of flu and influenza-like illnesses in the coming epidemic seasons. laboratory studies using molecular rt-pcr biology methods for detection of genetic material of influenza virus and other respiratory viruses were performed by voivod ... | 2015 | 25252895 |
| detection of respiratory tract pathogens with molecular biology methods. | this paper describes the use in routine diagnosis of virological kit, which was designed to identify the 15 most common respiratory viruses in clinical specimens of nasopharyngeal aspirates, swabs, and bronchoalveolar lavage. it is a one-step multiplex rt-pcr system for the detection of influenza virus type a and type b, human respiratory syncytial virus type a, b; human adenovirus, human metapneumovirus, human coronaviruses 229e/nl63 and oc43, human parainfluenza type 1, 2, 3, human rhinovirus ... | 2015 | 25252894 |
| common questions about outpatient care of premature infants. | preterm births (deliveries before 37 weeks' gestation) comprise 12% of all u.s. births and are responsible for onethird of all infant deaths. neonatal medical advances have increased survival, and primary care physicians often care for infants who were in the neonatal intensive care unit after birth. functions of the primary care physician include coordination of medical and social services, nutritional surveillance, and managing conditions associated with prematurity. parental guidance and enco ... | 2014 | 25250998 |
| refining the balance of attenuation and immunogenicity of respiratory syncytial virus by targeted codon deoptimization of virulence genes. | respiratory syncytial virus (rsv) is the most important pathogen for lower respiratory tract illness in children for which there is no licensed vaccine. live-attenuated rsv vaccines are the most clinically advanced in children, but achieving an optimal balance of attenuation and immunogenicity is challenging. one way to potentially retain or enhance immunogenicity of attenuated virus is to mutate virulence genes that suppress host immune responses. the ns1 and ns2 virulence genes of the rsv a2 s ... | 2014 | 25249281 |
| presentation of severe combined immunodeficiency with respiratory syncytial virus and pneumocystis co-infection. | severe combined immunodeficiency can cause severe, life-threatening viral, bacterial and fungal infections at an early age. we report a case of a 4-month-old boy with co-infection by respiratory syncytial virus and pneumocystis jiroveci infection that led to recognition of severe combined immunodeficiency. | 2015 | 25247585 |
| seroprevalence of respiratory syncytial virus igg among healthy young adults in basic training for the republic of korea air force. | this investigation enrolled 570 healthy young males gathered from all over the country for military service at the republic of korea air force boot camp. it confirmed rsv igg seroprevalence by utilizing the enzyme immunoassay method just prior to undergoing basic training. the mean age of this study was 20.25 ± 1.34 yr old. the results of their immunoassay seroprofiles showed that 561 men (98.4%) were positive, 2 (0.4%) were negative and 7 (1.2%) were equivocal belonging to the grey zone. it was ... | 2014 | 25246755 |
| evaluation of a rapid antigen detection point-of-care test for respiratory syncytial virus and influenza in a pediatric hospitalized population in the netherlands. | this pilot study evaluates the diagnostic performance of sofia rsv fluorescent immunoassay analyzer (fia) and sofia influenza a + b fia for rapid detection of respiratory syncytial virus and influenza a and b. sofia had a lower-than-expected sensitivity for all viruses and a high rate of false-positive results for influenza b virus. | 2014 | 25241640 |
| additive protection induced by mixed virus-like particles presenting respiratory syncytial virus fusion or attachment glycoproteins. | respiratory syncytial virus (rsv) is the most important pathogen for lower respiratory tract illness in infants and a high priority for vaccine development. we previously reported that rsv virus-like particles (vlps) expressing either the fusion (f) or attachment (g) glycoprotein could confer protection against rsv challenge in balb/c mice. here, we tested the hypothesis that rsv vlp vaccine efficacy can be enhanced by mixing rsv vlp f and rsv vlp g, and we analyzed host responses to these rsv v ... | 2014 | 25239522 |
| an outbreak of acute respiratory disease in china caused by human adenovirus type b55 in a physical training facility. | to investigate the cause of an acute respiratory tract infection (arti) outbreak. | 2014 | 25236387 |
| caesarean section and hospitalization for respiratory syncytial virus infection: a population-based study. | hospitalization for respiratory syncytial virus (rsv) infection and asthma share common determinants, and meta-analyses indicate that children delivered by caesarean section (cs) are at increased risk of asthma. we aimed to investigate whether birth by cs is associated with an increased risk of hospitalization for rsv illness. | 2015 | 25232778 |
| human respiratory syncytial virus infection is inhibited by ifn-induced transmembrane proteins. | the ifn immune system plays an essential role in protecting the host against most viral infections. in order to explore the interactions between the ifn pathway and human respiratory syncytial virus (rsv) infection, and to identify potential ifn-stimulated genes (isgs) that may be involved in suppressing the replication of rsv, we utilized an ifn pathway-specific microarray to study the effects of rsv infection on the ifn pathway in hela cells. we showed that rsv infection enhanced the expressio ... | 2015 | 25228491 |
| double-stapled respiratory syncytial virus may prevent nasopulmonary infection. | 2014 | 25226620 | |
| the use of a multiplex real-time pcr assay for diagnosing acute respiratory viral infections in children attending an emergency unit. | the use of a multiplex molecular technique to identify the etiological pathogen of respiratory viral infections might be a support as clinical signs are not characteristic. | 2014 | 25223920 |
| cd8+ t cell immunity against human respiratory syncytial virus. | human respiratory syncytial virus (hrsv) was first discovered in the 1950s, but despite decades of research, a licensed vaccine against it is not available. epidemiological studies indicate that antibodies directed against the fusion protein (f) partially correlate with protection. in addition, an f-specific monoclonal antibody is licensed as a prophylactic treatment in children who are at high risk of developing complications following hrsv infection. therefore, most hrsv-oriented vaccination s ... | 2014 | 25223272 |
| apnea induced by respiratory syncytial virus infection is not associated with viral invasion of the central nervous system. | we aimed to study whether direct central nervous system invasion is responsible for the neurologic manifestations seen in hospitalized infants with respiratory syncytial virus (rsv) infection. cerebrospinal fluid from infants with rsv infection was tested for the detection of the following respiratory rna viruses: rsv, influenza a and b, pandemic influenza h1n1, parainfluenza-3, human metapneumovirus, adenovirus, parechovirus and enterovirus. all children tested negative for the presence of vira ... | 2014 | 25222310 |
| hospital length-of-stay is associated with rhinovirus etiology of bronchiolitis. | to determine whether hospital length-of-stay (los) for bronchiolitis is influenced by the causative virus: respiratory syncytial virus (rsv) or rhinovirus. | 2014 | 25222302 |
| interferon response and respiratory virus control are preserved in bronchial epithelial cells in asthma. | some investigators find a deficiency in ifn production from airway epithelial cells infected with human rhinovirus in asthma, but whether this abnormality occurs with other respiratory viruses is uncertain. | 2014 | 25216987 |
| is radiological appearance of lower respiratory tract infection due to respiratory syncytial virus a predictor of clinical outcome? | respiratory syncytial virus (rsv) lower respiratory tract infection is the most common viral infection in childhood. rsv-infected infants demonstrate various radiographic findings. the aim of the study is to evaluate whether assessment of radiological characteristics of lower tract infection due to rsv may be a predictor of clinical outcome in newborns. | 2015 | 25211292 |
| comparison of the simplexa™ flu a/b & rsv kit (nucleic acid extraction-dependent assay) and the prodessa proflu+™ assay for detecting influenza and respiratory syncytial viruses. | the relative performance of 2 widely used reverse transcription polymerase chain reaction (rt-pcr) assays, the focus diagnostics simplexa™ flu a/b & rsv kit (nucleic acid extraction-dependent assay) and the prodessa proflu+™ assay, was evaluated using 735 prospectively and retrospectively collected nasopharyngeal swab specimens. overall, the assays showed positive and negative agreements of 100% and 99.7% for influenza a, 98.1% and 99.9% for influenza b, and 99.3% and 99.5% for respiratory syncy ... | 2014 | 25209363 |
| symptomatic and asymptomatic respiratory viral infections in the first year of life: association with acute otitis media development. | sensitive diagnostic assays have increased the detection of viruses in asymptomatic individuals. the clinical significance of asymptomatic respiratory viral infection in infants is unknown. | 2015 | 25205769 |
| [analysis of coinfections detected among respiratory syncytial virus subtypes and other respiratory viruses]. | 2015 | 25205177 | |
| [epidemiological study of respiratory viruses detected in patients under two years old who required admission because of lower respiratory disease]. | the etiologic role of recently identified respiratory viruses for lower respiratory tract disease (lrtd) remains unclear in japan. the purpose of this study was to investigate the prevalence of respiratory viruses in young children with lrtd. we prospectively examined 721 children who were under two years old and admitted to a single medical center in japan with lrtd between april 2007 and march 2012. viral nucleotic acids were taken from their nasal swabs. each sample was examined with (rt-) pc ... | 2014 | 25199375 |
| performance of the simplexa™ flu a/b & rsv direct kit on respiratory samples collected in saline solution. | molecular assays for diagnosis of influenza a, influenza b, and respiratory syncytial virus (rsv) with short turnaround time are of considerable clinical importance. we have evaluated the diagnostic performance of the simplexa(™) flu a/b & rsv direct kit, which has a run time of 60 min, using different types of respiratory samples collected from patients with a suspected respiratory tract infection, including materials not previously evaluated on this kit. | 2014 | 25195649 |
| respiratory syncytial virus: clinical and epidemiological pattern in pediatric patients admitted to a children's hospital between 2000 and 2013. | respiratory syncytial virus (rsv) is the major causative organism associated with acute lower respiratory tract infections in children.the objective of this study was to describe the clinical and epidemiological pattern of rsv and identify risk factors for rsv infection. | 2014 | 25192519 |
| [nosocomial respiratory viral infection]. | nosocomial viral infections have not been well studied. in this context, the aim of this work is to review epidemiological and therapeutic data published on the main viral agents that can produce nosocomial respiratory infection. the study thus aims to expand knowledge of behaviour of these agents in nosocomial infections and provide information to improve the implementation of preventive measures. the concepts of mimivirus, herpes simplex virus, varicella-zoster virus, cytomegalovirus, respirat ... | 2014 | 25189985 |
| rsv infection among children born moderately preterm in a community-based cohort. | we aimed to determine the rates of proven respiratory syncytial virus (rsv) hospitalization and disease severity among children born moderately preterm (mp, gestational age [ga] 32-36 weeks, n = 964), children born full-term (ft, ga 38-42 weeks, n = 572), and children born early preterm (ep, ga <32 weeks, n = 524). our second aim was to identify risk factors for rsv hospitalization among mp. we extracted data from parental questionnaires and medical records, retrieved from a community-based coho ... | 2015 | 25189655 |
| comparison of three commercial rt-pcr systems for the detection of respiratory viruses. | due to the insensitivity of rapid tests for respiratory viruses, nucleic acid amplification tests are quickly becoming the standard of care. | 2014 | 25183359 |
| epidemiologic differences of four major respiratory viruses between children, adolescents, and adults in korea. | the purpose of this study was to investigate the epidemiology of four major respiratory viruses among the korean population. this retrospective study was conducted over four years, from january 2005 to december 2008. among a total of 23,806 specimens, 5512 virus isolates underwent culture for influenza a and b viruses (ifa/b), parainfluenza virus (piv), respiratory syncytial virus (rsv), and adenovirus (adv). patients were divided into two groups: children/adolescents and adults. the viruses det ... | 2014 | 25179390 |
| nosocomial respiratory syncytial virus infections in the palivizumab-prophylaxis era with implications regarding high-risk infants. | although respiratory syncytial virus (rsv) infection continues to be a leading cause of infant hospitalization with a high transmission rate, recent data on nosocomial rsv infection are scarce. this study investigated the clinical and epidemiologic characteristics of nosocomial rsv infection in the palivizumab-prophylaxis era. | 2014 | 25179332 |
| [viral etiologies of hospitalized pneumonia patients aged less than five years in six provinces, 2009-2012]. | to analyze the viral etiologies of hospitalized pneumonia patients aged less than five years in six provinces during 2009-2012, and to describe the seasonality of the detected viral etiologies. | 2014 | 25174464 |
| recombinant bovine adenovirus-3 co-expressing bovine respiratory syncytial virus glycoprotein g and truncated glycoprotein gd of bovine herpesvirus-1 induce immune responses in cotton rats. | one of the impediments in the development of safe and cost effective vaccines for veterinary use has been the availability of appropriate delivery vehicle. we have chosen to develop and use bovine adenovirus (badv)-3 as vaccine delivery vector in cattle. here, we describe the construction of recombinant e3 deleted badv-3 vectors expressing single vaccine antigen (bav360; bovine respiratory syncytial virus g) or two vaccine antigens (bav851; bovine herpesvirus-1gdt and bovine respiratory syncytia ... | 2015 | 25173687 |
| baculovirus-expressed virus-like particle vaccine in combination with dna encoding the fusion protein confers protection against respiratory syncytial virus. | respiratory syncytial virus (rsv) is a major viral agent causing significant morbidity and mortality in young infants and the elderly. there is no licensed vaccine against rsv and it is a high priority to develop a safe rsv vaccine. we determined the immunogenicity and protective efficacy of combined virus-like particle and dna vaccines presenting rsv glycoproteins (fd.vlp) in comparison with formalin inactivated rsv (fi-rsv). immunization of mice with fd.vlp induced higher ratios of igg2a/igg1 ... | 2014 | 25173478 |
| maternal immunization with chimpanzee adenovirus expressing rsv fusion protein protects against neonatal rsv pulmonary infection. | respiratory syncytial virus (rsv) is a leading cause of lower respiratory tract disease with high morbidity and mortality in young infants and children. despite numerous efforts, a licensed vaccine against rsv remains elusive. since young infants form the primary target group of rsv disease, maternal immunization to boost the protection in neonates is an attractive strategy. in this study we tested the efficacy of maternal immunization with a chimpanzee adenovirus expressing codon-optimized rsv ... | 2014 | 25171847 |
| viral infections and the development of asthma in children. | viral aetiology, host susceptibility (in particular allergic predisposition and sensitization), and illness severity, timing and frequency all appear to contribute as synergistic factors to the risk of developing asthma. experimental models have shown both innate and adaptive immune responses contribute to this risk with lung inflammatory cells showing marked differences in phenotype and function in young compared with older animals, and these differences are further enhanced following virus inf ... | 2013 | 25165549 |
| [application possibility of the macrolides for the rs virus infections]. | human respiratory syncytial virus (rsv) is a major causative agent of respiratory infections. common cold syndrome caused by rs virus, which is prevalent in winter, occasionally develops into bronchitis, bronchiolitis and pneumonia. in particular, severe rs virus infections in infants with underlying cardiopulmonary diseases and with low-birth weight baby have been a problem. furthermore, repetitive rs virus infections occur for the entire lifetime, and the relationship between rs virus infectio ... | 2014 | 25163248 |
| when to perform urine cultures in respiratory syncytial virus-positive febrile older infants? | respiratory syncytial virus (rsv) infections are associated with clinically significant rate of urinary tract infections (utis) in young infants. previous research investigating rsv infections and utis has been performed mainly in infants younger than 2 to 3 months and has not focused on the risk of uti in infants 3 to 12 months. | 2014 | 25162692 |
| attenuation of human respiratory syncytial virus by genome-scale codon-pair deoptimization. | human respiratory syncytial virus (rsv) is the most important viral agent of serious pediatric respiratory-tract disease worldwide. a vaccine or generally effective antiviral drug is not yet available. we designed new live attenuated rsv vaccine candidates by codon-pair deoptimization (cpd). specifically, viral orfs were recoded by rearranging existing synonymous codons to increase the content of underrepresented codon pairs. amino acid coding was completely unchanged. four cpd rsv genomes were ... | 2014 | 25157129 |
| randomized, double-blind study of the safety of the liquid versus lyophilized formulation of palivizumab in premature infants and children with chronic lung disease of prematurity. | to avoid the need for reconstitution required by lyophilized palivizumab, a liquid formulation was developed. this study assessed the safety and antidrug antibodies (ada) of the liquid formulation of palivizumab compared with the lyophilized formulation. | 2014 | 25156956 |
| infection with novel respiratory syncytial virus genotype ontario (on1) in adult hematopoietic cell transplant recipients, texas, 2011-2013. | respiratory syncytial virus (rsv) can cause severe respiratory disease in adult hematopoietic cell transplant (hct) recipients. rsv subgroups a and b have evolved into multiple genotypes. we report on a recently described rsv genotype (on1) in a cohort of adult hct recipients in texas. | 2015 | 25156562 |
| impact of a rapid respiratory panel test on patient outcomes. | evolution of polymerase chain reaction testing for infectious pathogens has occurred concurrent with a focus on value-based medicine. | 2015 | 25152311 |
| challenges and future in vaccines, drug development, and immunomodulatory therapy. | pulmonary diseases and infections are among the top contributors to human morbidity and mortality worldwide, and despite the successful history of vaccines and antimicrobial therapeutics, infectious disease still presents a significant threat to human health. effective vaccines are frequently unavailable in developing countries, and successful vaccines have yet to be developed for major global maladies, such as tuberculosis. furthermore, antibiotic resistance poses a growing threat to human heal ... | 2014 | 25148426 |
| oral gs-5806 activity in a respiratory syncytial virus challenge study. | respiratory syncytial virus (rsv) is a common cause of infant hospitalizations and is increasingly recognized as a cause of considerable morbidity and mortality. no accepted antiviral treatment exists. | 2014 | 25140957 |
| 'they said it was bronchiolitis; is it going to turn into asthma doctor?'. | acute bronchiolitis is a common paediatric disease of infancy. its association with subsequent asthma development has puzzled clinicians and epidemiologists for decades. this article reviews the current state of knowledge regarding the role of acute bronchiolitis in the inception of asthma. there is little doubt that acute bronchiolitis is associated with an increased risk of recurrent wheezing throughout the primary school years although the direction of causality--i.e. whether bronchiolitis in ... | 2014 | 25138566 |
| the burden of influenza and rsv among inpatients and outpatients in rural western kenya, 2009-2012. | in kenya, detailed data on the age-specific burden of influenza and rsv are essential to inform use of limited vaccination and treatment resources. | 2014 | 25133576 |
| respiratory syncytial virus infection in high-risk infants - an update on palivizumab prophylaxis. | morbidity due to respiratory syncytial virus (rsv) disease is still high in infants and children worldwide during the first two to five years of life. certain categories of high-risk infants with increased morbidity and mortality attributed to rsv disease have been identified and are included in national recommendations for prophylaxis with the monoclonal rsv antibody palivizumab. most guidelines recommend palivizumab for preterm infants born less than or equal to 32 weeks gestational age with o ... | 2014 | 25132870 |
| lactate dehydrogenase and caspase activity in nasopharyngeal secretions are predictors of bronchiolitis severity. | bronchiolitis is the leading cause of hospitalization in infants. biomarkers of disease severity might help in clinical management. | 2014 | 25132512 |
| alleviation of respiratory syncytial virus replication and inflammation by fungal immunomodulatory protein fip-fve from flammulina velutipes. | respiratory syncytial virus (rsv) causes bronchiolitis in children followed by inflammation and asthma-like symptoms. the development of preventive therapy for this virus continues to pose a challenge. fungal immunomodulatory proteins (fips) exhibit anti-inflammatory function. fip-fve is an immunomodulatory protein isolated from flammulina velutipes. to determine whether fip-fve affects the infection or consequence of immunity of rsv, we investigated viral titers of rsv and inflammatory cytokine ... | 2014 | 25131377 |
| a colorimetric immunoassay for respiratory syncytial virus detection based on gold nanoparticles-graphene oxide hybrids with mercury-enhanced peroxidase-like activity. | a novel colorimetric immunoassay for highly sensitive detection of respiratory syncytial virus (rsv), one of the leading causes of severe lower respiratory tract infections in all age groups, has been proposed based on hg(2+)-stimulated peroxidase-like activity of gold nanoparticles-graphene oxide (aunps-go) hybrids. this metal ion-enhanced immunoassay shows high promise in the field of biomedical sciences. | 2014 | 25131342 |
| ifn-γ ca microsatellite polymorphism is associated with susceptibility to respiratory syncytial virus infection and severity. | ifn-γ, an essential cytokine in the viral cell-mediated immune response, has been associated with the pathogenesis of respiratory syncytial virus (rsv) bronchiolitis and to the severity of the infection. the aim of this study was to investigate whether ifn-γ ca microsatellite (rs3138557) polymorphism was associated with susceptibility to rsv in chinese han children and with the severity of the infection. | 2014 | 25130433 |
| respiratory viruses are continuously detected in children with chronic tonsillitis throughout the year. | to evaluate the oscillations on the viral detection in adenotonsillar tissues from patients with chronic adenotonsillar diseases as an indicia of the presence of persistent viral infections or acute subclinical infections. | 2014 | 25128448 |
| [modelling respiratory syncytial virus (rsv) transmission children aged less than five years-old]. | studying respiratory syncytial virus (rsv) transmission dynamics in a population of children aged less than 5 years-old using deterministic models. | 2014 | 25124134 |
| epidemiological characteristics and immune status of children with respiratory syncytial virus. | respiratory syncytial virus (rsv) infections are the dominant cause of pneumonia in children. in order to determine the epidemiological characteristics and immune status of children with respiratory syncytial virus, a prospective study was performed among patients with rsv infection. comparisons between rsv pneumonia group and normal control group, rsv pneumonia group had lower il-2 (median levels, pg/ml: 3.8 vs. 5.1, p < 0.01), and higher il-4 (median levels, pg/ml: 3.2 vs. 2.4, p < 0.01), il-1 ... | 2015 | 25123681 |
| methyltransferase-defective avian metapneumovirus vaccines provide complete protection against challenge with the homologous colorado strain and the heterologous minnesota strain. | avian metapneumovirus (ampv), also known as avian pneumovirus or turkey rhinotracheitis virus, is the causative agent of turkey rhinotracheitis and is associated with swollen head syndrome in chickens. since its discovery in the 1970s, ampv has been recognized as an economically important pathogen in the poultry industry worldwide. the conserved region vi (cr vi) of the large (l) polymerase proteins of paramyxoviruses catalyzes methyltransferase (mtase) activities that typically methylate viral ... | 2014 | 25122790 |
| respiratory infections in children up to two years of age on prophylaxis with palivizumab. | to identify the viruses involved in acute respiratory tract infections and to analyze the rates of hospitalization and death in children on palivizumab prophylaxis. | 2014 | 25119744 |
| immunoprophylaxis against respiratory syncytial virus with palvizumab: what is new? | 2014 | 25119743 | |
| comparison of severe pneumonia caused by human metapneumovirus and respiratory syncytial virus in hospitalized children. | the objective of this study is to compare the incidence and clinical characteristics of severe pneumonia caused by human metapneumovirus (hmpv) to respiratory syncytial virus (rsv) infection in children. | 2014 | 25118733 |
| induction of long-term immunity against respiratory syncytial virus glycoprotein by an osmotic polymeric nanocarrier. | respiratory syncytial virus (rsv) is one of the most common causes of viral deaths in infants worldwide, yet no effective vaccines are available. here, we report an osmotically active polysaccharide-based polysorbitol transporter (pst) prepared from sorbitol diacrylate and low-molecular-weight polyethylenimine (pei) showing a potent, yet safe, adjuvant activity and acting as an effective delivery tool for rsv glycoprotein (rgp) antigen. pst showed no toxicity in vitro or in vivo, unlike pei and ... | 2014 | 25110285 |
| co-immunization with virus-like particle and dna vaccines induces protection against respiratory syncytial virus infection and bronchiolitis. | this study demonstrates that immunization with non-replicating virus-like particle (ffg vlp) containing rsv f and g glycoproteins together with rsv f dna induced t helper type 1 antibody responses to rsv f similar to live rsv infection. upon rsv challenge 21weeks after immunization, ffg vlp vaccination induced protection against rsv infection as shown by clearance of lung viral loads, and the absence of eosinophil infiltrates, and did not cause lung pathology. in contrast, formalin-inactivated r ... | 2014 | 25110201 |
| the genetic variability of glycoproteins among respiratory syncytial virus subtype a in china between 2009 and 2013. | human respiratory syncytial virus (rsv) is the leading cause of acute lower respiratory tract infections in infants and children under 5years of age. the novel genotype on1 has a 72-nucleotide duplication, which is the largest duplicated genome portion of rsv. whether the on1 genotype will follow the pattern of the ba genotype, which has a 60-nucleotide duplication, and become the predominant rsv-a strain is a global concern. to obtain information regarding the prevalence of the on1 genotype in ... | 2014 | 25109878 |
| virus-like nanoparticle and dna vaccination confers protection against respiratory syncytial virus by modulating innate and adaptive immune cells. | respiratory syncytial virus (rsv) is an important human pathogen. expression of virus structural proteins produces self-assembled virus-like nanoparticles (vlp). we investigated immune phenotypes after rsv challenge of immunized mice with vlp containing rsv f and g glycoproteins mixed with f-dna (fdfg vlp). in contrast to formalin-inactivated rsv (fi-rsv) causing vaccination-associated eosinophilia, fdfg vlp immunization induced low bronchoalveolar cellularity, higher ratios of cd11c(+) versus c ... | 2015 | 25109662 |
| identification of cellular proteins that interact with newcastle disease virus and human respiratory syncytial virus by a two-dimensional virus overlay protein binding assay (vopba). | although it is well documented that the initial attachment receptors for newcastle disease virus (ndv) and respiratory syncytial virus (rsv) are sialic acid-containing molecules and glycosaminoglycans respectively, the exact nature of the receptors for both viruses remains to be deciphered. moreover, additional molecules at the host cell surface might be involved in the entry mechanism. with the aim of identifying the cellular proteins that interact with ndv and rsv at the cell surface, we perfo ... | 2014 | 25109545 |
| nitazoxanide: a first-in-class broad-spectrum antiviral agent. | originally developed and commercialized as an antiprotozoal agent, nitazoxanide was later identified as a first-in-class broad-spectrum antiviral drug and has been repurposed for the treatment of influenza. a phase 2b/3 clinical trial recently published in the lancet infectious diseases found that oral administration of nitazoxanide 600mg twice daily for five days reduced the duration of clinical symptoms and reduced viral shedding compared to placebo in persons with laboratory-confirmed influen ... | 2014 | 25108173 |
| development of a reverse genetics system for respiratory syncytial virus long strain and an immunogenicity study of the recombinant virus. | respiratory syncytial virus (rsv) is an important human respiratory pathogen, particularly of infants and older adults, and despite several decades of research and development, no licensed vaccine is available. studies have confirmed that enhancement of rsv disease does not occur after inoculation with rsv live-attenuated vaccine candidates, making such vaccines preferable. in this paper, reverse genetics was used to construct two recombinant viruses, a recombinant long strain (rlong) and rlong- ... | 2014 | 25107552 |
| a systematic review of predictive modeling for bronchiolitis. | bronchiolitis is the most common cause of illness leading to hospitalization in young children. at present, many bronchiolitis management decisions are made subjectively, leading to significant practice variation among hospitals and physicians caring for children with bronchiolitis. to standardize care for bronchiolitis, researchers have proposed various models to predict the disease course to help determine a proper management plan. this paper reviews the existing state of the art of predictive ... | 2014 | 25106933 |
| a comprehensive proteomic view of responses of a549 type ii alveolar epithelial cells to human respiratory syncytial virus infection. | human respiratory syncytial virus is a major respiratory pathogen for which there are no suitable antivirals or vaccines. a better understanding of the host cell response to this virus may redress this problem. the present report concerns analysis of multiple independent biological replicates of control and 24 h infected lysates of a549 cells by two different proteomic workflows. one workflow involved fractionation of lysates by in-solution protein ief and individual fractions were digested usin ... | 2014 | 25106423 |
| paramyxovirus glycoprotein incorporation, assembly and budding: a three way dance for infectious particle production. | paramyxoviruses are a family of negative sense rna viruses whose members cause serious diseases in humans, such as measles virus, mumps virus and respiratory syncytial virus; and in animals, such as newcastle disease virus and rinderpest virus. paramyxovirus particles form by assembly of the viral matrix protein, the ribonucleoprotein complex and the surface glycoproteins at the plasma membrane of infected cells and subsequent viral budding. two major glycoproteins expressed on the viral envelop ... | 2014 | 25105277 |
| the underrecognized burden of respiratory syncytial virus among infants presenting to us emergency departments. | 2015 | 25104728 | |
| programming of the lung by early-life infection. | many important human diseases, such as asthma, have their developmental origins in early life. respiratory infections in particular may alter the course of asthma and may either protect against or promote the development of this disease. it is likely that the nature of the effects depends on the type and age of infection and is determined by the impact of infection on the immune and respiratory systems. immunity in early life is plastic and can be moulded by antigen encounter, which may enhance ... | 2012 | 25102006 |