Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| complete genome sequences of human respiratory syncytial virus genotype a and b isolates from south korea. | there is a paucity of complete genome sequence information for human respiratory syncytial virus (hrsv). to this end, we sequenced the complete genome sequences of hrsv genotype a (hrsv-a/ic688/12) and genotype b (hrsv-b/gw0047/14 and hrsv-b/ic0027/14). this information will increase the understanding of hrsv genetic diversity, evolution, pathogenicity, antigenicity, and transmissibility. | 2015 | 25908140 |
| the impact of viral dynamics on the clinical severity of infants with respiratory syncytial virus bronchiolitis. | the impact of dynamic respiratory syncytial virus (rsv) load on the clinical severity of hospitalized infants with bronchiolitis has not been clarified. nasopharyngeal aspirates were obtained from 60 infants who were diagnosed with bronchiolitis within 96 hr of wheezing onset upon admission and on days 3, 5, and 7 in the hospital, and 17 respiratory viruses were detected. the rsv load was quantified by real-time qpcr for rsv subtypes a and b at different time points. scoring criteria were used t ... | 2015 | 25907780 |
| rt-bst: an integrated approach for reverse transcription and enrichment of cdna from viral rna. | the synthesis of cdna from rna is challenging due to the inefficiency of reverse transcription (rt). in order to address this, an rt-bst method was developed for sequential rt of rna and bst dna polymerase amplification for enrichment of cdna in a single-tube reaction. using genomic rna from bacteriophage ms2, the yield of cdna produced by rt alone and rt-bst were compared by analysis of polymerase chain reaction (pcr)-amplified products. a superior performance was observed when amplifying ms2 c ... | 2015 | 25906484 |
| detection of viral and bacterial pathogens in hospitalized children with acute respiratory illnesses, chongqing, 2009-2013. | acute respiratory infections (aris) cause large disease burden each year. the codetection of viral and bacterial pathogens is quite common; however, the significance for clinical severity remains controversial. we aimed to identify viruses and bacteria in hospitalized children with ari and the impact of mixed detections.hospitalized children with ari aged ≤16 were recruited from 2009 to 2013 at the children's hospital of chongqing medical university, chongqing, china. nasopharyngeal aspirates (n ... | 2015 | 25906103 |
| high viral load and respiratory failure in adults hospitalized for respiratory syncytial virus infections. | a prospective study among adults hospitalized for polymerase chain reaction-confirmed respiratory syncytial virus infections (n = 123) showed frequent occurrence of lower respiratory-tract complications causing respiratory insufficiency (52.8%), requirement for assisted ventilation (16.3%), and intensive care unit admission/death (12.2%). high viral rna concentration was detected at time of hospitalization, including in patients who presented later than 2 days of illness (day 1-2, 7.29 ± 1.47; d ... | 2015 | 25904604 |
| mixed infection of peste des petits ruminants virus (pprv) and other respiratory viruses in dromedary camels in sudan, an abattoir study. | this study was intended to determine the role played by peste des petits ruminants (ppr) in causing respiratory infections in camels and its association with other respiratory viruses. a total of 474 lung specimens showing pneumonia were collected from clinically healthy camels in slaughterhouses at five different areas in sudan. using immunocapture elisa (icelisa), 214 specimens (45.1 %) were found to be positive for ppr antigen. the highest prevalence was found in central sudan (59.9 %) then n ... | 2015 | 25904508 |
| editorial commentary: can a reduced-dose prophylaxis schedule provide adequate coverage against respiratory syncytial virus infection? | 2015 | 25904371 | |
| reduced-dose schedule of prophylaxis based on local data provides near-optimal protection against respiratory syncytial virus. | respiratory syncytial virus (rsv) is a major cause of respiratory infections among young children and can lead to severe disease among some infants. infants at high risk for severe rsv infection receive monthly injections of a prophylactic monoclonal antibody during the rsv season based on national guidelines. we considered whether a reduced-dose schedule tailored to the local rsv season in the continental united states would provide adequate protection. | 2015 | 25904370 |
| viral coinfection in acute respiratory infection in mexican children treated by the emergency service: a cross-sectional study. | acute respiratory infections (aris) cause illness. children under five years of age are highly vulnerable to these infections. viral coinfection or multiple viral infection is a variable that can have a significant impact on the evolution of these diseases. | 2015 | 25903455 |
| murine immune responses to virus-like particle-associated pre- and postfusion forms of the respiratory syncytial virus f protein. | virus-like particles (vlps) built on the newcastle disease virus (ndv) core proteins, np and m, and containing two chimeric proteins, f/f and h/g, composed of respiratory syncytial virus (rsv) fusion protein (f) and glycoprotein (g) ectodomains fused to the transmembrane and cytoplasmic domains of the ndv f and hn proteins, respectively, stimulate durable, protective rsv neutralizing antibodies in mice. here, we report the properties of vlps constructed to contain mutant rsv f protein ectodomain ... | 2015 | 25903340 |
| the importance of bacterial and viral infections associated with adult asthma exacerbations in clinical practice. | viral infection is one of the risk factors for asthma exacerbation. however, which pathogens are related to asthma exacerbation in adults remains unclear. | 2015 | 25901797 |
| monoclonal antibodies against the human respiratory syncytial virus obtained by immunization with epitope peptides and cpg-dna-liposome complex. | respiratory syncytial virus (rsv) is the major cause of pulmonary inflammation in infants, young children, and immunocompromised adults. however, the rsv vaccine is not yet available commercially. the rsv-f glycoprotein mediates virus-host cell fusion, leading to syncytial formation; therefore, the rsv-f glycoprotein has been a treatment target for prevention and therapy of rsv infection. to produce the rsv-f-protein epitope-specific monoclonal antibody (mab), balb/c mice were immunized with a c ... | 2015 | 25897608 |
| alveolar macrophage-derived type i interferons orchestrate innate immunity to rsv through recruitment of antiviral monocytes. | type i interferons (ifns) are important for host defense from viral infections, acting to restrict viral production in infected cells and to promote antiviral immune responses. however, the type i ifn system has also been associated with severe lung inflammatory disease in response to respiratory syncytial virus (rsv). which cells produce type i ifns upon rsv infection and how this directs immune responses to the virus, and potentially results in pathological inflammation, is unclear. here, we s ... | 2015 | 25897172 |
| influenza and other respiratory viruses detected by influenza-like illness surveillance in leyte island, the philippines, 2010-2013. | this study aimed to determine the role of influenza-like illness (ili) surveillance conducted on leyte island, the philippines, including involvement of other respiratory viruses, from 2010 to 2013. ili surveillance was conducted from january 2010 to march 2013 with 3 sentinel sites located in tacloban city, palo and tanauan of leyte island. ili was defined as fever ≥38°c or feverish feeling and either cough or running nose in a patient of any age. influenza virus and other 5 respiratory viruses ... | 2015 | 25893441 |
| structured performance assessment in three pediatric emergency scenarios: a validation study. | to develop and validate 3 performance evaluation checklists (pecs) for systematic performance assessment in 3 clinical scenarios: cardiopulmonary arrest, dyspnea with oxygen desaturation after intubation, and respiratory syncytial virus (rsv). | 2015 | 25890675 |
| a bovine respiratory syncytial virus model with high clinical expression in calves with specific passive immunity. | bovine respiratory syncytial virus (brsv) is a major cause of respiratory disease in cattle worldwide. calves are particularly affected, even with low to moderate levels of brsv-specific maternally derived antibodies (mda). available brsv vaccines have suboptimal efficacy in calves with mda, and published infection models in this target group are lacking in clinical expression. here, we refine and characterize such a model. | 2015 | 25890239 |
| identification of viral and bacterial pathogens from hospitalized children with severe acute respiratory illness in lusaka, zambia, 2011-2012: a cross-sectional study. | morbidity and mortality from respiratory infections are higher in resource-limited countries than developed countries, but limited studies have been conducted in resource-limited settings to examine pathogens from patients with acute respiratory infections. influenza surveillance has been conducted in zambia since 2008; however, only 4.3% of patients enrolled in 2011-2012 were positive for influenza. therefore, we examined non-influenza respiratory pathogens in children with severe acute respira ... | 2015 | 25888024 |
| viral infections in outpatients with medically attended acute respiratory illness during the 2012-2013 influenza season. | while it is known that acute respiratory illness (ari) is caused by an array of viruses, less is known about co-detections and the resultant comparative symptoms and illness burden. this study examined the co-detections, the distribution of viruses, symptoms, and illness burden associated with ari between december 2012 and march 2013. | 2015 | 25887948 |
| host response to respiratory syncytial virus infection. | respiratory syncytial virus (rsv) infection is the leading cause of bronchiolitis and hospitalization in young infants and causes 100, 000-200, 000 deaths annually. there is still no licensed vaccine against rsv infection and the therapeutic options are mainly supportive. despite almost six decades of research, important knowledge gaps remain with respect to the characterization of immune mechanisms responsible for protection and pathogenesis, as well as to the identification of risk factors tha ... | 2015 | 25887611 |
| molecular characterization of influenza viruses collected from young children in uberlandia, brazil - from 2001 to 2010. | influenza remains a major health problem due to the seasonal epidemics that occur every year caused by the emergence of new influenza virus strains. hemagglutinin (ha) and neuraminidase (na) glycoproteins are under selective pressure and subjected to frequent changes by antigenic drift. therefore, our main objective was to investigate the influenza cases in uberlândia city, midwestern brazil, in order to monitor the appearance of new viral strains, despite the availability of a prophylactic vacc ... | 2015 | 25886886 |
| nasal mucosal microrna expression in children with respiratory syncytial virus infection. | respiratory syncytial virus (rsv) infection is a common cause of pediatric hospitalization. microrna, key regulators of the immune system, have not previously been investigated in respiratory specimens during viral infection. we investigated microrna expression in the nasal mucosa of 42 rsv-positive infants, also comparing microrna expression between disease severity subgroups. | 2015 | 25884957 |
| transmission of respiratory syncytial virus infection within families. | background. because the production of an effective respiratory syncytial virus (rsv) vaccine for infants is challenging, vaccination of other family members is one viable alternative to prevent severe rsv illnesses in infants. methods. in a prospective study, we enrolled all family members of children who were hospitalized with rsv infection. nasal swabs for rsv detection were obtained from all participating family members. data on respiratory symptoms in the family members prior to and after ... | 2015 | 25884006 |
| structural virology. near-atomic cryo-em structure of the helical measles virus nucleocapsid. | measles is a highly contagious human disease. we used cryo-electron microscopy and single particle-based helical image analysis to determine the structure of the helical nucleocapsid formed by the folded domain of the measles virus nucleoprotein encapsidating an rna at a resolution of 4.3 angstroms. the resulting pseudoatomic model of the measles virus nucleocapsid offers important insights into the mechanism of the helical polymerization of nucleocapsids of negative-strand rna viruses, in parti ... | 2015 | 25883315 |
| dose-response models for selected respiratory infectious agents: bordetella pertussis, group a streptococcus, rhinovirus and respiratory syncytial virus. | dose-response assessment is one step in quantitative microbial risk assessment (qmra). four infectious microbes capable of causing respiratory diseases important to public health, and for which dose-response functions have not been available are: bordetella pertussis (whooping cough), group a streptococcus (pharyngitis), rhinovirus (common cold) and respiratory syncytial virus (common cold). the objective of this study was to fit dose-response functions for these microbes to published experiment ... | 2015 | 25880210 |
| the aetiology of community associated pneumonia in children in nanjing, china and aetiological patterns associated with age and season. | viral and atypical bacterial pathogens play an important role in respiratory tract infection. using the pneumoslide igm test, the presented study explored the aetiology of community-acquired pneumonia and investigated further whether there was an association between age or season and aetiological organisms. | 2015 | 25879996 |
| severe acute respiratory infection in children in a densely populated urban slum in kenya, 2007-2011. | reducing acute respiratory infection burden in children in africa remains a major priority and challenge. we analyzed data from population-based infectious disease surveillance for severe acute respiratory illness (sari) among children <5 years of age in kibera, a densely populated urban slum in nairobi, kenya. | 2015 | 25879805 |
| mycoplasma pneumoniae and chlamydophila pneumoniae in hospitalized children with bronchiolitis. | bronchiolitis is an acute lower respiratory tract infection in early childhood caused mainly by different viruses. etiology of bronchiolitis have been studied in different environments and populations. respiratory syncytial virus (rsv), human metapneumovirus (hmpv), human bocavirus (hbov), human rhinoviruses (hrv) have consistently been shown to predominate. few studies however have attempted to determine whether other pathogens, particularly mycoplasma pneumoniae (mp) and chlamydophila pneumoni ... | 2015 | 25879563 |
| a network flow approach to predict protein targets and flavonoid backbones to treat respiratory syncytial virus infection. | respiratory syncytial virus (rsv) infection is the major cause of respiratory disease in lower respiratory tract in infants and young children. attempts to develop effective vaccines or pharmacological treatments to inhibit rsv infection without undesired effects on human health have been unsuccessful. however, rsv infection has been reported to be affected by flavonoids. the mechanisms underlying viral inhibition induced by these compounds are largely unknown, making the development of new drug ... | 2015 | 25879022 |
| receipt of palivizumab before birth hospitalization discharge among preterm infants in the united states. | this study aims to determine predischarge palivizumab receipt prevalence among infants ≤ 36 weeks' gestational age. | 2015 | 25877742 |
| viral bacterial co-infection of the respiratory tract during early childhood. | acute respiratory infection (ari) is an important cause of morbidity in children. mixed aetiology is frequent, with pathogenic viruses and bacteria co-detected in respiratory secretions. however, the clinical significance of these viral/bacterial co-infections has long been a controversial topic. while severe bacterial pneumonia following influenza infection has been well described, associations are less clear among infections caused by viruses that are more common in young children, such as res ... | 2015 | 25877546 |
| [clinical value of viral detection of liquid chip method in community-acquired pneumonia]. | to evaluate the clinical value of viral detection of liquid chip method in community-acquired pneumonia (cap). | 2015 | 25877241 |
| bronchiectasis exacerbations: the role of atypical bacteria, respiratory syncytial virus and pulmonary function tests. | aside from the known role of common bacteria, there is a paucity of data regarding the possible role of atypical bacteria and viruses in exacerbations of non-cystic fibrosis bronchiectasis. | 2015 | 25874735 |
| revealing the binding mode between respiratory syncytial virus fusion protein and benzimidazole-based inhibitors. | human respiratory syncytial virus (hrsv) is a major respiratory pathogen in newborn infants and young children and can also be a threat to some elderly and high-risk adults with chronic pulmonary disease and the severely immunocompromised. the rsv fusion (rsvf) protein has been an attractive target for vaccine and drug development. experimental results indicate a series of benzimidazole-based inhibitors which target rsvf protein to inhibit the viral entry of rsv. to reveal the binding mode betwe ... | 2015 | 25872614 |
| one-year prospective study of community acquired influenza and parainfluenza viral infections in hospitalized egyptian children with malignancy: single center experience. | respiratory viruses are widespread in the community and easily transmitted to immunocompromised patients. | 2015 | 25871509 |
| sedimentation equilibrium of a small oligomer-forming membrane protein: effect of histidine protonation on pentameric stability. | analytical ultracentrifugation (auc) can be used to study reversible interactions between macromolecules over a wide range of interaction strengths and under physiological conditions. this makes auc a method of choice to quantitatively assess stoichiometry and thermodynamics of homo- and hetero-association that are transient and reversible in biochemical processes. in the modality of sedimentation equilibrium (se), a balance between diffusion and sedimentation provides a profile as a function of ... | 2015 | 25867485 |
| molecular epidemiology and molecular characterization of respiratory syncytial viruses at a tertiary care university hospital in catalonia (spain) during the 2013-2014 season. | human respiratory syncytial virus (hrsv) is the main cause of lower respiratory tract infections among infants and young children. | 2015 | 25866332 |
| direct multiplexed whole genome sequencing of respiratory tract samples reveals full viral genomic information. | acute respiratory tract infections (rti) cause substantial morbidity during childhood, and are responsible for the majority of pediatric infectious diseases. although most acute rti are thought to be of viral origin, viral etiology is still unknown in a significant number of cases. | 2015 | 25866327 |
| poor performance characteristics of conventional pcr in detection of respiratory syncytial virus-experience of a tertiary care centre in southern india. | respiratory syncytial virus (rsv) is a significant cause of contagious acute respiratory infections in children and older adults. since there are contradictory reports regarding the efficacy of different methods to detect rsv, we evaluated the performance of the conventional pcr versus real-time pcr in 222 patients with acute respiratory infections (ari) recruited between january 2012 and march 2013. conventional pcr had a very poor sensitivity of 40% (95% ci: 19.2-63.9%) and failed to detect rs ... | 2015 | 25865982 |
| development of two salmonella-based oral vaccines against human respiratory syncytial virus. | human respiratory syncytial virus is the most common cause of bronchiolitis and other respiratory infections in infants and the elderly worldwide. we have developed two new oral vaccines using salmonella typhi ty21a to carry and express the immunogenic epitopes of rsv fusion (f) and attachment (g) glycoproteins on its surface, separately. to evaluate the efficacy of the designed vaccines, balb/c mice were orally immunized and then infected with rsv. immune response analyses showed that cellmedia ... | 2015 | 25864737 |
| a phenotypic high-throughput screen with rsv-infected primary human small airway epithelial cells (saecs). | respiratory syncytial virus (rsv) is a commonly occurring pathogen that can cause severe disease in children, the elderly, and immunocompromised individuals with a large, unmet clinical need. we developed a high-throughput, primary cell-based antiviral rsv assay to enable identification of small molecules using cytopathic effect (cpe) as a phenotypic end point. to provide increased biological relevance, we developed our assay with primary human small airway epithelial cells (saecs), which origin ... | 2015 | 25862685 |
| prevalence of rhinovirus and respiratory syncytial virus among patients with chronic rhinosinusitis. | chronic rhinosinusitis (crs) is one of the most common chronic illnesses, but the etiology and pathogenesis of crs are not well understood. few studies have been carried out on the role of viruses in patients with chronic sinusitis so far. | 2015 | 25861437 |
| adverse effects of bronchodilators in infants with bronchiolitis. | 2017 | 25859174 | |
| [association between moderate-severe bronchiolitis and syndrome of inappropriate antidiuretic hormone secretion in emergency departments]. | to identify clinical characteristics that may lead to the early recognition of patients admitted to the hospital for moderate-to-severe bronchiolitis with urine results associated with the syndrome of inappropriate antidiuretic hormone secretion (siadh). | 2016 | 25858009 |
| evaluating vaccination strategies for reducing infant respiratory syncytial virus infection in low-income settings. | respiratory syncytial virus (rsv) is a leading cause of lower respiratory tract disease and related hospitalization of young children in least developed countries. individuals are repeatedly infected, but it is the first exposure, often in early infancy, that results in the vast majority of severe rsv disease. unfortunately, due to immunological immaturity, infants are a problematic rsv vaccine target. several trials are ongoing to identify a suitable candidate vaccine and target group, but no i ... | 2015 | 25857701 |
| respiratory syncytial virus fusion protein promotes tlr-4-dependent neutrophil extracellular trap formation by human neutrophils. | acute viral bronchiolitis by respiratory syncytial virus (rsv) is the most common respiratory illness in children in the first year of life. rsv bronchiolitis generates large numbers of hospitalizations and an important burden to health systems. neutrophils and their products are present in the airways of rsv-infected patients who developed increased lung disease. neutrophil extracellular traps (nets) are formed by the release of granular and nuclear contents of neutrophils in the extracellular ... | 2015 | 25856628 |
| zinc binding activity of human metapneumovirus m2-1 protein is indispensable for viral replication and pathogenesis in vivo. | human metapneumovirus (hmpv) is a member of the pneumovirinae subfamily in the paramyxoviridae family that causes respiratory tract infections in humans. unlike members of the paramyxovirinae subfamily, the polymerase complex of pneumoviruses requires an additional cofactor, the m2-1 protein, which functions as a transcriptional antitermination factor. the m2-1 protein was found to incorporate zinc ions, although the specific role(s) of the zinc binding activity in viral replication and pathogen ... | 2015 | 25855728 |
| interaction between human bap31 and respiratory syncytial virus small hydrophobic (sh) protein. | the small hydrophobic (sh) protein is a short channel-forming polypeptide encoded by the human respiratory syncytial virus (hrsv). deletion of sh protein leads to the viral attenuation in mice and primates, and delayed apoptosis in infected cells. we have used a membrane-based yeast two-hybrid system (mby2h) and a library from human lung cdna to detect proteins that bind sh protein. this led to the identification of a membrane protein, b-cell associated protein 31 (bap31). transfected sh protein ... | 2015 | 25854864 |
| antiviral and antitumor activities of the lectin extracted from aspidistra elatior. | lectins, a group of highly diverse proteins of non-immune origin and are ubiquitously distributed in plants, animals and fungi, have multiple significant biological functions, such as anti-fungal, anti-viral and, most notably, anti-tumor activities. a lectin was purified from the rhizomes of aspidistra elatior blume, named a. elatior lectin (ael). in vitro experiments showed that the minimum inhibitory concentrations of ael against the vesicular stomatitis virus, coxsackie virus b4, and respirat ... | 2015 | 25854839 |
| respiratory syncytial virus infection in older adults: an under-recognized problem. | human respiratory syncytial virus (rsv) is an enveloped, single-stranded, negative-sense rna virus and member of the paramyxoviridae family of the genus pneumovirus that was first reported as a major pathogen in pediatric populations. however, since its discovery, rsv has not infrequently been detected in adults. reinfection occurs throughout life, with more severe disease occurring in older adults, immunocompromised patients, and those with underlying cardiopulmonary disease. initially describe ... | 2015 | 25851217 |
| clinical outcomes associated with respiratory virus detection before allogeneic hematopoietic stem cell transplant. | the management of respiratory virus infections prior to hematopoietic cell transplant (hct) is difficult. we examined whether respiratory virus detection before hct influenced the requirement for bronchoscopy, hospitalization, and overall survival following hct. | 2015 | 25847977 |
| respiratory syncytial virus, an ongoing medical dilemma: an expert commentary on respiratory syncytial virus prophylactic and therapeutic pharmaceuticals currently in clinical trials. | as the most important viral cause of severe respiratory disease in infants and increasing recognition as important in the elderly and immunocompromised, respiratory syncytial virus (rsv) is responsible for a massive health burden worldwide. prophylactic antibodies were successfully developed against rsv. however, their use is restricted to a small group of infants considered at high risk of severe rsv disease. there is still no specific therapeutics or vaccines to combat rsv. as such, it remains ... | 2015 | 25847510 |
| respiratory syncytial virus infection of airway cells: role of micrornas. | micrornas (mirnas) are small single-stranded rna molecules involved in the regulation of gene expression at the post-transcriptional level. in the airways, mirnas are implicated in the modulation of antiviral defense, through modulation of both innate and adaptive immune response in inflammatory and immune effector cells but also in parenchymal cells. the first target of respiratory viruses are airway epithelial cells. following infection, an altered expression of distinct mirnas occurs in airwa ... | 2015 | 25847505 |
| characteristics of respiratory syncytial virus-induced bronchiolitis co-infection with mycoplasma pneumoniae and add-on therapy with montelukast. | the influence of mycoplasma pneumoniae (mp) infection on bronchiolitis remains unclear. additionally, reports on the efficacies of leukotriene receptor antagonists in the treatment of bronchiolitis have been inconclusive. | 2016 | 25846070 |
| [acute tubulointerstitial nephritis following adenovirus and respiratory syncytial virus infection]. | acute tubulointerstitial nephritis (tin) is responsible for nearly 10% of acute renal failure (arf) cases in children. it is mostly drug-induced, but in a few cases viruses are involved, probably by an indirect mechanism. an immune-competent 13-month-old boy was admitted to the intensive care unit for severe arf with anuria in a context of fever, cough, and rhinorrhea lasting 1 week. the kidney biopsy performed early brought out tubulointerstitial damage with mild infiltrate of lymphocytes, with ... | 2015 | 25842199 |
| correction for kim et al., development of an adenovirus-based respiratory syncytial virus vaccine: preclinical evaluation of efficacy, immunogenicity, and enhanced disease in a cotton rat model. | 2015 | 25841050 | |
| effect of young sibling visitation on respiratory syncytial virus activity in a nicu. | to determine whether the restriction of young sibling (<13 years) visitation in the neonatal intensive care unit (nicu) during the respiratory syncytial virus (rsv) season was associated with a reduction in the rate of rsv infection among nicu patients. | 2015 | 25836315 |
| respiratory syncytial virus infection of primary human mast cells induces the selective production of type i interferons, cxcl10, and ccl4. | respiratory syncytial virus (rsv) causes severe respiratory tract infections, which might have a role in the development of airway hyperreactivity. mast cells are important effector cells in allergy, with sentinel cell roles in host defense. however, the role of mast cells in response to rsv infection is unknown. | 2015 | 25819983 |
| neutralizing epitopes on the respiratory syncytial virus fusion glycoprotein. | respiratory syncytial virus (rsv) is a leading cause of pneumonia and bronchiolitis, but despite decades of research a safe and effective vaccine has remained elusive. the viral fusion glycoprotein (rsv f) plays an obligatory role in the entry process and is the major target of neutralizing antibodies, making it an attractive target for vaccine development. this review will summarize the recently determined structures of rsv f in the prefusion and postfusion conformations and describe the locati ... | 2015 | 25819327 |
| viral etiology of acute lower respiratory tract infections in hospitalized young children in a children's referral hospital in iran. | viruses are considered major causes of acute respiratory tract infections among children under 5 years old. in this study we investigated the prevalence of three respiratory viruses--respiratory syncytial virus (rsv), influenza virus (inf) and adenovirus (adv)--among hospitalized children with acute viral lower respiratory tract infections (lrtis). nasopharyngeal aspirates were collected from children under five who had been hospitalized for lrtis. the clinical data, including demographic data ( ... | 2015 | 25818953 |
| [approach to a respiratory syncytial virus outbreak related to medical care in hematology]. | 2015 | 25817393 | |
| viral etiology of community-acquired pneumonia among adolescents and adults with mild or moderate severity and its relation to age and severity. | better knowledge of distribution of respiratory viruses (rvs) in adolescents and adults with community-acquired pneumonia (cap) is needed. | 2015 | 25812108 |
| self-sampling for analysis of respiratory viruses in a large-scale epidemiological study in sweden. | viral diagnosis of respiratory tract infections has so far required sampling by health professionals,hampering large-scale epidemiological studies of virus-specific disease outcomes. as part of a population-based, prospective study of work-related risk factors for transmission of viral infections (swede-i), we developed a scheme for self-sampling with nasal swabs. random selection from the gainfully employed population of a medium-sized town in central sweden resulted in a study cohort of 2,237 ... | 2015 | 25811646 |
| deaths associated with respiratory syncytial and influenza viruses among persons ≥5 years of age in hiv-prevalent area, south africa, 1998-2009(1). | we estimated deaths attributable to influenza and respiratory syncytial virus (rsv) among persons >5 years of age in south africa during 1998-2009 by applying regression models to monthly deaths and laboratory surveillance data. rates were expressed per 100,000 person-years. the mean annual number of seasonal influenza-associated deaths was 9,093 (rate 21.6). persons >65 years of age and hiv-positive persons accounted for 50% (n = 4,552) and 28% (n = 2,564) of overall seasonal influenza-associat ... | 2015 | 25811455 |
| small hydrophobic protein of respiratory syncytial virus as a novel vaccine antigen. | 2015 | 25804473 | |
| bd veritor system respiratory syncytial virus rapid antigen detection test: point-of-care results in primary care pediatric offices compared with reverse transcriptase polymerase chain reaction and viral culture methods. | during respiratory syncytial virus season, many children present to pediatric offices and urgent care medical facilities with cough, tachypnea, intercostal retraction, wheezing, as well as disturbed appetite and sleep. identification of the responsible viral pathogen is quite difficult because several pathogens can produce similar signs and symptoms. | 2015 | 25803752 |
| superinfection between influenza and rsv alternating patterns in san luis potosí state, méxico. | the objective of this paper is to explain through the ecological hypothesis superinfection and competitive interaction between two viral populations and niche (host) availability, the alternating patterns of respiratory syncytial virus (rsv) and influenza observed in a regional hospital in san luis potosí state, méxico using a mathematical model as a methodological tool. the data analyzed consists of community-based and hospital-based acute respiratory infections (ari) consultations provided by ... | 2015 | 25803450 |
| diagnosis of viral infections using myxovirus resistance protein a (mxa). | myxoma resistance protein 1 (mxa) is induced during viral infections. mxa testing could be helpful to differentiate between viral and bacterial infections. | 2015 | 25802344 |
| association between respiratory viruses and exacerbation of copd: a case-control study. | respiratory viral infection is the main cause of exacerbations of chronic obstructive pulmonary disease (copd) in all age groups. the present study aimed to find out the association between viral infection in exacerbated and stable patients with copd as well as evaluating the frequency of respiratory viruses in the iranian exacerbated patients. | 2015 | 25800059 |
| decapping protein 1 phosphorylation modulates il-8 expression during respiratory syncytial virus infection. | respiratory syncytial virus (rsv) is a negative-strand rna virus that is an important cause of bronchiolitis and pneumonia. we investigated the effect of rsv infection on the expression patterns of cellular proteins involved in regulating mrna translation and degradation, and found that a processing-body protein involved in mrna degradation, decapping protein 1a (dcp1), was phosphorylated rapidly following infection. uv-inactivated and sucrose-purified rsv were sufficient to mediate dcp1 phospho ... | 2015 | 25796077 |
| titanium dioxide nanoparticles exacerbate pneumonia in respiratory syncytial virus (rsv)-infected mice. | to reveal the effects of tio2 nanoparticles, used in cosmetics and building materials, on the immune response, a respiratory syncytial virus (rsv) infection mouse model was used. balb/c mice were exposed once intranasally to tio2 at 0.5mg/kg and infected intranasally with rsv five days later. the levels of ifn-γ and chemokine ccl5, representative markers of pneumonia, in the bronchoalveolar lavage fluids of rsv-infected mice had increased significantly in tio2-exposed mice compared with the cont ... | 2015 | 25795424 |
| sequencing and analysis of globally obtained human respiratory syncytial virus a and b genomes. | human respiratory syncytial virus (rsv) is the leading cause of respiratory tract infections in children globally, with nearly all children experiencing at least one infection by the age of two. partial sequencing of the attachment glycoprotein gene is conducted routinely for genotyping, but relatively few whole genome sequences are available for rsv. the goal of our study was to sequence the genomes of rsv strains collected from multiple countries to further understand the global diversity of r ... | 2015 | 25793751 |
| a novel respiratory syncytial virus (rsv) f subunit vaccine adjuvanted with gla-se elicits robust protective th1-type humoral and cellular immunity in rodent models. | illness associated with respiratory syncytial virus (rsv) remains an unmet medical need in both full-term infants and older adults. the fusion glycoprotein (f) of rsv, which plays a key role in rsv infection and is a target of neutralizing antibodies, is an attractive vaccine target for inducing rsv-specific immunity. | 2015 | 25793508 |
| in vitro evaluation of the antiviral properties of shilajit and investigation of its mechanisms of action. | shilajit, a herbomineral substance exuded from rocks in steep mountainous regions, has been used for thousands of years by the indian ayurvedic and siddha systems of traditional medicine to relieve ailments and enhance quality of life. although a large number of therapeutic properties have been ascribed to shilajit, its therapeutic potential is still largely unexplored by modern research and many of its claimed bioactivities lack scientific validation. the present study was undertaken to investi ... | 2015 | 25792012 |
| comparison of bronchiolitis of human metapneumovirus and human respiratory syncytial virus. | 2015 | 25790057 | |
| clinical translation of rnai-based treatments for respiratory diseases. | the ability to harness the rna interference (rnai) mechanism as a potential potent therapeutic has attracted great interest from academia and industry. numerous preclinical and recent clinical trials have demonstrated the effectiveness of rnai triggers such as synthetic small interfering rna (sirna). chemical modification and delivery technologies can be utilized to avoid immune stimulation and improve the bioactivity and pharmacokinetics. local application to the respiratory epithelia allows di ... | 2013 | 25787868 |
| vaccination with human papillomavirus pseudovirus-encapsidated plasmids targeted to skin using microneedles. | human papilloma virus-like particles (hpv vlp) serve as the basis of the current licensed vaccines for hpv. we have previously shown that encapsidation of dna expressing the model antigen m/m2 from respiratory syncytial virus (rsv) in hpv pseudovirions (psv) is immunogenic when delivered intravaginally. because the hpv capsids confer tropism for basal epithelium, they represent attractive carriers for vaccination targeted to the skin using microneedles. in this study we asked: 1) whether hpv16 v ... | 2015 | 25785935 |
| value of a risk scoring tool to predict respiratory syncytial virus disease severity and need for hospitalization in term infants. | several environmental and demographic risk factors have been validated and are used to determine the risk of acquiring severe respiratory syncytial virus (rsv) infection and subsequent hospitalization in late preterm infants born at 33-35 weeks gestational age. the applicability of the same composite model of risk factors in the term population has not been fully explored. the primary objective of this pilot study was to establish whether a risk scoring tool (rst), could predict the severity of ... | 2015 | 25784593 |
| incidence of respiratory viruses in peruvian children with acute respiratory infections. | acute respiratory infections are responsible for high morbi-mortality in peruvian children. however, the etiological agents are poorly identified. this study, conducted during the pandemic outbreak of h1n1 influenza in 2009, aims to determine the main etiological agents responsible for acute respiratory infections in children from lima, peru. nasopharyngeal swabs collected from 717 children with acute respiratory infections between january 2009 and december 2010 were analyzed by multiplex rt-pcr ... | 2015 | 25784285 |
| the caspase inhibitor zvad increases lung inflammation in pneumovirus infection in mice. | severe respiratory syncytial virus (rsv) disease is a frequent cause of acute respiratory distress syndrome (ards) in young children, and is associated with marked lung epithelial injury and neutrophilic inflammation. experimental studies on ards have shown that inhibition of apoptosis in the lungs reduces lung epithelial injury. however, the blockade of apoptosis in the lungs may also have deleterious effects by hampering viral clearance, and importantly, by enhancing or prolonging local proinf ... | 2015 | 25780096 |
| continuous positive airway pressure with helmet versus mask in infants with bronchiolitis: an rct. | noninvasive continuous positive airway pressure (cpap) is usually applied with a nasal or facial mask to treat mild acute respiratory failure (arf) in infants. a pediatric helmet has now been introduced in clinical practice to deliver cpap. this study compared treatment failure rates during cpap delivered by helmet or facial mask in infants with respiratory syncytial virus-induced arf. | 2015 | 25780074 |
| leukotriene inhibitors for bronchiolitis in infants and young children. | bronchiolitis is an acute inflammatory illness of the bronchioles common among infants and young children. it is often caused by the respiratory syncytial virus (rsv). management of bronchiolitis varies between clinicians, reflecting the lack of evidence for a specific treatment approach. the leukotriene pathway has been reported to be involved in the pathogenesis of bronchiolitis. leukotriene inhibitors such as montelukast have been used in infants and young children with bronchiolitis. however ... | 2015 | 25773054 |
| development and laboratory evaluation of a real-time pcr assay for detecting viruses and bacteria of relevance for community-acquired pneumonia. | community-acquired pneumonia may present with similar clinical symptoms, regardless of viral or bacterial cause. diagnostic assays are needed to rapidly discriminate between causes, because this will guide decisions on appropriate treatment. therefore, a quantitative real-time pcr (qpcr) assay with duplex reactions targeting eight bacteria and six viruses was developed. technical performance was examined with linear plasmids. upper and lower respiratory tract specimens were used to compare the q ... | 2015 | 25772704 |
| rsv vaccine-enhanced disease is orchestrated by the combined actions of distinct cd4 t cell subsets. | there is no currently licensed vaccine for respiratory syncytial virus (rsv) despite being the leading cause of lower respiratory tract infections in children. children previously immunized with a formalin-inactivated rsv (fi-rsv) vaccine exhibited enhanced respiratory disease following natural rsv infection. subsequent studies in animal models have implicated roles for cd4 t cells, eosinophils and non-neutralizing antibodies in mediating enhanced respiratory disease. however, the underlying imm ... | 2015 | 25769044 |
| safety and pharmacokinetics of extended use of palivizumab in saudi arabian infants and children. | the peak season of respiratory syncytial virus (rsv) infections in warmer climates may extend beyond the typical five-month rsv season of temperate regions. additional monthly doses of palivizumab may be necessary in warmer regions to protect children at high risk for serious infection by the rsv. | 2015 | 25767550 |
| head-to-head comparison of the diagnostic accuracies of bd veritor™ system rsv and quidel® sofia® rsv fia systems for respiratory syncytial virus (rsv) diagnosis. | respiratory syncytial virus (rsv) is one of the most common causes of severe lower respiratory tract disease among infants and young children. bd veritor™ system rsv (bd) and quidel(®) sofia(®) rsv fia (qd) are the new generation lateral flow digital immunoassay (dia) tests with an instrumented read for the qualitative detection of rsv viral antigens. | 2015 | 25766995 |
| molecular characterization of respiratory syncytial viruses infecting children reported to have received palivizumab immunoprophylaxis. | respiratory syncytial virus (rsv) is a major cause of respiratory infections in children. palivizumab (pz) is the only rsv-specific immunoprophylaxis approved by the u.s. food and drug administration. mutations leading to amino acid substitutions in the pz binding site of the rsv f protein have been associated with breakthrough rsv infections in patients receiving pz. | 2015 | 25766983 |
| [acute conjunctivitis caused by respiratory syncytial virus a group]. | 2015 | 25764947 | |
| history of passive antibody administration for prevention and treatment of infectious diseases. | we describe the history of passive immunization to provide context for the series of articles to follow. the history of passive immunization with antibodies to prevent or treat infectious diseases is a story of different eras. there was an extraordinary era of discovery and clinical implementation before the chemical nature of antibodies was even known. this empirical process provided the resources and reagents used to describe and characterize humoral immunity, better define the chemical proper ... | 2015 | 25760933 |
| etiology of pediatric fever in western kenya: a case-control study of falciparum malaria, respiratory viruses, and streptococcal pharyngitis. | in kenya, more than 10 million episodes of acute febrile illness are treated annually among children under 5 years. most are clinically managed as malaria without parasitological confirmation. there is an unmet need to describe pathogen-specific etiologies of fever. we enrolled 370 febrile children and 184 healthy controls. we report demographic and clinical characteristics of patients with plasmodium falciparum, group a streptococcal (gas) pharyngitis, and respiratory viruses (influenza a and b ... | 2015 | 25758648 |
| vector-mediated antibody gene transfer for infectious diseases. | this chapter discusses the emerging field of vector-mediated antibody gene transfer as an alternative vaccine for infectious disease, with a specific focus on hiv. however, this methodology need not be confined to hiv-1; the general strategy of vector-mediated antibody gene transfer can be applied to other difficult vaccine targets like hepatitis c virus, malaria, respiratory syncytial virus, and tuberculosis. this approach is an improvement over classical passive immunization strategies that ad ... | 2015 | 25757620 |
| [recombinant human interferon alpha 2b broad-spectrum anti-respiratory viruses pharmacodynamics study in vitro]. | this study is to investigate the effect of recombinant human interferon alpha 2b against broad-spectrum respiratory viruses in vitro. at the cellular level, the effect of the recombinant human interferon alpha 2b on influenza a virus was detected using real-time fluorescence quantitative rt-pcr. the effects of the recombinant human interferon alpha 2b on influenza b virus, parainfluenza virus, respiratory syncytial virus (rsv) and coronavirus were detected using cytopathic effect (cpe) method. i ... | 2014 | 25757280 |
| a pharmacological profile of ribavirin and monitoring of its plasma concentration in chronic hepatitis c infection. | chronic hepatitis c (chc) infection, usually an asymptomatic infection, has long-term serious complications such as cirrhosis, hepatocellular carcinoma, and end-stage liver disease requiring liver transplantation (lt). several novel drugs against hepatitis c which form part of 'specifically targeted antiviral therapy for hepatitis c' (stat-c) have been developed. these include ns3/4a protease inhibitors telaprevir, boceprevir, and nucleoside/non-nucleoside polymerase inhibitors (ns5a) which hold ... | 2012 | 25755405 |
| oral ribavirin for respiratory syncytial virus infection after lung transplantation: efficacy and cost-efficiency. | respiratory syncytial virus (rsv) causes serious respiratory tract infections in lung transplant (ltx) recipients, is associated with development of bronchiolitis obliterans syndrome, and has no proven effective therapy. we evaluated the efficacy, safety, and cost-effectiveness of oral ribavirin for the treatment of rsv infection after ltx. | 2015 | 25753833 |
| prevalence and correlation of infectious agents in hospitalized children with acute respiratory tract infections in central china. | acute respiratory tract infections (artis) are associated with significant morbidity and mortality worldwide, especially in children under the age of 5 years. almost 2 million children die from artis each year, and most of them are from developing countries. the prevalence and correlation of pathogens in artis are poorly understood, but are critical for improving case prevention, treatment, and management. in this study, we investigated the prevalence and correlation of infectious agents in chil ... | 2015 | 25751402 |
| palivizumab epitope-displaying virus-like particles protect rodents from rsv challenge. | respiratory syncytial virus (rsv) is the most common cause of serious viral bronchiolitis in infants, young children, and the elderly. currently, there is not an fda-approved vaccine available for rsv, though the mab palivizumab is licensed to reduce the incidence of rsv disease in premature or at-risk infants. the palivizumab epitope is a well-characterized, approximately 24-aa helix-loop-helix structure on the rsv fusion (f) protein (f254-277). here, we genetically inserted this epitope and mu ... | 2015 | 25751145 |
| poor outcome of acute respiratory infection in young children with underlying health condition in brazil. | it is well established that respiratory viruses are an important cause of hospitalizations in young children worldwide, but data are limited on the contribution of specific viruses to severe illness in south america. we describe clinical and laboratory findings from prospective surveillance for acute respiratory infections at a tertiary hospital in são paulo, brazil. | 2015 | 25747778 |
| respiratory syncytial virus genotypes circulating in urban ghana: february to november 2006. | respiratory syncytial virus (rsv) is the major cause of acute lower respiratory tract infection (alri) in young children. rsv strains have been divided into 2 major antigenic groups (a and b), which are further divided into several genotypes, but very little is known about its circulating genotypes in ghana. this study characterized rsv genotypes detected in children with alri in accra between february and november 2006. | 2014 | 25745535 |
| genetic variability in g2 and f2 region between biological clones of human respiratory syncytial virus with or without host immune selection pressure. | human respiratory syncytial virus (hrsv) is an important respiratory pathogens among children between zero-five years old. host immunity and viral genetic variability are important factors that can make vaccine production difficult. in this work, differences between biological clones of hrsv were detected in clinical samples in the absence and presence of serum collected from children in the convalescent phase of the illness and from their biological mothers. viral clones were selected by plaque ... | 2015 | 25742274 |
| intensive care unit admission rates for respiratory syncytial virus infection as a function of age in preterm infants born at 32-35-week gestation and not receiving immunoprophylaxis. | 2015 | 25742086 | |
| cesarean section and hospitalization for respiratory syncytial virus infection. | 2015 | 25741978 |