Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| prospective evaluation of rapid antigen tests for diagnosis of respiratory viral pathogens. | acute respiratory infection is a frequently transmitted illness of concern to doctors and patients. considering its airborne transmission, early diagnosis of such disease is particularly important. this study explored respiratory viral infections with influenza virus, parainfluenza virus, respiratory syncytial virus, human metapneumovirus, human bocavirus, coronavirus, and other early diagnostic substances as confirmed by literature resources. this study also used the corresponding monoclonal an ... | 2015 | 26293052 |
| detection and characterization of respiratory viruses causing acute respiratory illness and asthma exacerbation in children during three different season (2011-2014) in mexico city. | viral infections play a significant role as cause of acute respiratory infections (aris) and exacerbations of chronic diseases. aris are now the leading cause of mortality in children worldwide, especially in developing countries. recently human rhinovirus (hrv) infection has been emerged as an important cause of pneumonia and asthma exacerbation. | 2015 | 26289993 |
| interleukin-4 polymorphism is associated with severity of respiratory syncytial virus infection. | respiratory syncytial virus (rsv) infection is a major health problem in young children. this study aimed to investigate whether the interleukin (il)-4 -590c/t and -33 c/t polymorphisms were associated with the susceptibility to rsv infection in chinese han children and with the severity of the infection. | 2016 | 26289664 |
| matrix metalloproteinase 9 exerts antiviral activity against respiratory syncytial virus. | increased lung levels of matrix metalloproteinase 9 (mmp9) are frequently observed during respiratory syncytial virus (rsv) infection and elevated mmp9 concentrations are associated with severe disease. however little is known of the functional role of mmp9 during lung infection with rsv. to determine whether mmp9 exerted direct antiviral potential, active mmp9 was incubated with rsv, which showed that mmp9 directly prevented rsv infectivity to airway epithelial cells. using knockout mice the ef ... | 2015 | 26284919 |
| analytical performance of the automated multianalyte point-of-care maripoc® for the detection of respiratory viruses. | the analytical performance of maripoc® respi test (arcdia® laboratories, turku, finland) was evaluated using nucleic acid amplification techniques (naats) as the gold standard. the maripoc assay allows automated detection of antigens from 8 respiratory viruses: influenza a and b viruses, respiratory syncytial virus, adenovirus, human metapneumovirus, and parainfluenza viruses 1-3. positive results from samples with high viral load are available in 20min. nasopharyngeal aspirates (n=192) from pat ... | 2015 | 26283523 |
| a review of the cd4+ t cell contribution to lung infection, inflammation and repair with a focus on wheeze and asthma in the pediatric population. | childhood asthma and wheezing are very common, especially in those born preterm. genetic and environmental factors are associated with developing asthma and wheezing. respiratory syncytial virus and rhinovirus infections have been implicated in playing a causal role in the development of childhood asthma and wheezing, perhaps by altering the development of the immune system. several subtypes of asthma and wheezing have been described which involve different mechanisms of pathophysiology. much of ... | 2017 | 26280024 |
| identification of ccl2, rarres2 and efnb2 as host cell factors that influence the multistep replication of respiratory syncytial virus. | human respiratory syncytial virus (rsv) is a major causative agent of respiratory tract infections in children worldwide. preterm children or those with underlying cardiopulmonary disorders are at particularly high risk of developing severe and lethal rsv respiratory tract infections; however, there are currently no effective vaccines or anti-viral drugs. to identify targets for the development of drugs to treat rsv infections, we investigated host cell factors involved in the replication of rsv ... | 2015 | 26277777 |
| neutrophils in respiratory syncytial virus infection: a target for asthma prevention. | lower respiratory tract infections by respiratory syncytial virus (rsv) are the foremost cause of infant hospitalization and are implicated in lasting pulmonary impairment and the development of asthma. neutrophils infiltrate the airways of pediatric patients with rsv-induced bronchiolitis in vast numbers: approximately 80% of infiltrated cells are neutrophils. however, why neutrophils are recruited to the site of viral respiratory tract infection is not clear. in this review we discuss the bene ... | 2015 | 26277597 |
| generation of monoclonal antibodies specific of the postfusion conformation of the pneumovirinae fusion (f) protein. | paramyxovirus entry into cells requires fusion of the viral and cell membranes mediated by one of the major virus glycoproteins, the fusion (f) glycoprotein which transits from a metastable pre-fusion conformation to a highly stable post-fusion structure during the membrane fusion process. f protein refolding involves large conformational changes of the protein trimer. one of these changes results in assembly of two heptad repeat sequences (hra and hrb) from each protomer into a six-helix bundle ... | 2015 | 26275682 |
| use and interpretation of a rapid respiratory syncytial virus antigen detection test among infants hospitalized in a neonatal intensive care unit - wisconsin, march 2015. | on march 25, 2015, the wisconsin division of public health was notified of a possible respiratory syncytial virus (rsv) infection outbreak among infants hospitalized in a neonatal intensive care unit (nicu). on march 23, the index patient (neonate a), aged 3 days, had feeding intolerance and apnea. a nasopharyngeal swab specimen collected from neonate a was tested using a single-manufacturer rapid rsv antigen detection test (rradt) at the hospital laboratory; the result was positive. the followi ... | 2015 | 26270063 |
| systems biology from virus to humans. | natural infection and then recovery are considered to be the most effective means for hosts to build protective immunity. thus, mimicking natural infection of pathogens, many live attenuated vaccines such as influenza virus, and yellow fever vaccine 17d were developed and have been successfully used to induce protective immunity. however, humans fail to generate long-term protective immunity to some pathogens after natural infection such as influenza virus, respiratory syncytial virus (rsv), and ... | 2017 | 26269748 |
| efficacy of a virus-vectored vaccine against human and bovine respiratory syncytial virus infections. | human respiratory syncytial virus (hrsv) is a major cause of lower respiratory tract disease in children and the elderly for which there is still no effective vaccine. we have previously shown that panad3-rsv, which is a chimpanzee adenovirus-vectored vaccine candidate that expresses a secreted form of the hrsv f protein together with the n and m2-1 proteins of hrsv, is immunogenic in rodents and nonhuman primates, and protects mice and cotton rats from hrsv challenge. because the extent to whic ... | 2015 | 26268314 |
| chimpanzee adenovirus- and mva-vectored respiratory syncytial virus vaccine is safe and immunogenic in adults. | respiratory syncytial virus (rsv) causes respiratory infection in annual epidemics, with infants and the elderly at particular risk of developing severe disease and death. however, despite its importance, no vaccine exists. the chimpanzee adenovirus, panad3-rsv, and modified vaccinia virus ankara, mva-rsv, are replication-defective viral vectors encoding the rsv fusion (f), nucleocapsid (n), and matrix (m2-1) proteins for the induction of humoral and cellular responses. we performed an open-labe ... | 2015 | 26268313 |
| impact of human rhinovirus types and viral load on the severity of illness in hospitalized children with lower respiratory tract infections. | human rhinovirus (hrv) is not only responsible for at least one-half of all common colds but also associated with bronchitis, bronchiolitis, pneumonia and acute asthma exacerbation. however, the impact of different hrv types and viral load on disease severity has not been thoroughly elucidated. | 2015 | 26267309 |
| the role of th17 and treg responses in the pathogenesis of rsv infection. | the respiratory syncytial virus (rsv) represents the leading cause of viral bronchiolitis and pneumonia in children worldwide and is associated with high morbidity, hospitalization rate, and significant mortality rates. the immune response elicited by rsv is one of the main factors contributing to the pathogenesis of the disease. two subsets of the cellular immune response, the t helper 17 cell (th17) and the regulatory t-cell (treg), and more particularly the balance between these two subsets, ... | 2015 | 26267154 |
| matrix metalloproteinase-9 mediates rsv infection in vitro and in vivo. | respiratory syncytial virus (rsv) is an important human pathogen associated with substantial morbidity and mortality. the present study tested the hypothesis that rsv infection would increase matrix metalloproteinase (mmp)-9 expression, and that mmp-9 inhibition would decrease rsv replication both in vitro and in vivo. rsv a2 infection of human bronchial epithelial cells increased mmp-9 mrna and protein release. cells transfected with sirna against mmp-9 following rsv infection had lower viral t ... | 2015 | 26264019 |
| associations between viral and bacterial potential pathogens in the nasopharynx of children with and without respiratory symptoms. | nasopharyngeal (np) bacterial colonization is necessary for subsequent respiratory and/or invasive infection. our study aimed at comparing np bacterial colonization rates between children with and without symptoms of an acute viral respiratory tract infection and examining associations between identified microorganisms. | 2015 | 26262821 |
| from bronchiolitis guideline to practice: a critical care perspective. | acute viral bronchiolitis is a leading cause of admission to pediatric intensive care units, but research on the care of these critically ill infants has been limited. pathology of viral bronchiolitis revealed respiratory obstruction due to intraluminal debris and edema of the airways and vasculature. this and clinical evidence suggest that airway clearance interventions such as hypertonic saline nebulizers and pulmonary toilet devices may be of benefit, particularly in situations of atelectasis ... | 2015 | 26261767 |
| in silico structure-based design and synthesis of novel anti-rsv compounds. | respiratory syncytial virus (rsv) is the major cause for respiratory tract disease in infants and young children. currently, no licensed vaccine or a selective antiviral drug against rsv infections are available. here, we describe a structure-based drug design approach that led to the synthesis of a novel series of zinc-ejecting compounds active against rsv replication. 30 compounds, sharing a common dithiocarbamate moiety, were designed and prepared to target the zinc finger motif of the m2-1 p ... | 2015 | 26259810 |
| a randomized, blinded, controlled, dose-ranging study of a respiratory syncytial virus recombinant fusion (f) nanoparticle vaccine in healthy women of childbearing age. | respiratory syncytial virus (rsv) is a leading cause of infant morbidity and mortality. a recombinant rsv fusion protein nanoparticle vaccine (rsv f vaccine) candidate for maternal immunization was tested for safety and immunogenicity in women of childbearing age. | 2016 | 26259809 |
| antibodies enhance cxcl10 production during rsv infection of infant and adult immune cells. | respiratory syncytial virus (rsv) bronchiolitis is a major burden in infants below three months of age, when the primary immune response is mainly dependent on innate immunity and maternal antibodies. we investigated the influence of antibodies on innate immunity during rsv infection. pbmcs from infants and adults were stimulated with live rsv and inactivated rsv in combination with antibody-containing and antibody-depleted serum. the immune response was determined by transcriptome analysis and ... | 2015 | 26253531 |
| human respiratory syncytial virus non-structural protein ns1 modifies mir-24 expression via transforming growth factor-β. | human respiratory syncytial virus (rsv) is a major health challenge in the young and elderly owing to the lack of a safe and effective vaccine and proven antiviral drugs. understanding the mechanisms by which viral genes and proteins modulate the host response to infection is critical for identifying novel disease intervention strategies. in this study, the rsv non-structural protein ns1 was shown to suppress mir-24 expression during infection. lack of ns1 was linked to increased expression of m ... | 2015 | 26253191 |
| expression of chicken interleukin-2 by a highly virulent strain of newcastle disease virus leads to decreased systemic viral load but does not significantly affect mortality in chickens. | in mammals, interleukin 2 (il-2) has been shown to decrease replication or attenuate pathogenicity of numerous viral pathogens (herpes simplex virus, vaccinia virus, human respiratory syncytial virus, human immunodeficiency virus) by activating natural killer cells (nk), cytotoxic t lymphocytes and expanding subsets of memory cells. in chickens, il-2 has been shown to activate t cells, and as such it might have the potential to affect replication and pathogenesis of newcastle disease virus (ndv) ... | 2015 | 26253150 |
| alcohol during pregnancy worsens acute respiratory infections in children. | this study explored whether alcohol consumption during pregnancy increased the risk of life-threatening respiratory infections in children. | 2015 | 26249835 |
| the changing landscape of respiratory syncytial virus. | recognition of the acute and chronic burden of respiratory syncytial virus (rsv) lower respiratory tract infections (lrti) sparked a wave of initiatives to develop preventive and therapeutic products against the pathogen in recent years. rsv is a leading cause of hospitalization in infants in industrialized and developing countries, has been causally linked to recurrent wheezing during childhood, associated with pediatric asthma, and is an important cause of mortality in the first months of life ... | 2015 | 26247900 |
| a druggable pocket at the nucleocapsid/phosphoprotein interaction site of human respiratory syncytial virus. | presently, respiratory syncytial virus (rsv), the main cause of severe respiratory infections in infants, cannot be treated efficiently with antivirals. however, its rna-dependent polymerase complex offers potential targets for rsv-specific drugs. this includes the recognition of its template, the ribonucleoprotein complex (rnp), consisting of genomic rna encapsidated by the rsv nucleoprotein, n. this recognition proceeds via interaction between the phosphoprotein p, which is the main polymerase ... | 2015 | 26246564 |
| performance evaluation of puritan® universal transport system (unitranz-rt™) for preservation and transport of clinical viruses. | the ability of a non-propagating microbial transport medium to maintain the viability of clinically relevant viruses was compared to a similar commercial medium to establish performance equivalence. two dilutions of stock of test viruses, namely adenovirus (adv), cytomegalovirus (cmv), echovirus type 30 (ev), herpes simplex virus (hsv) types 1 and 2, influenza a, parainfluenza 3 (piv), respiratory syncytial virus (rsv), and varicella zoster virus (vzv), were spiked into puritan® medical products ... | 2015 | 26243168 |
| impact of placental malaria and hypergammaglobulinemia on transplacental transfer of respiratory syncytial virus antibody in papua new guinea. | passively acquired respiratory syncytial virus (rsv) neutralizing antibody protects against rsv-associated lower respiratory infections, but placental malaria (pm) and maternal hypergammaglobulinemia might interfere with transplacental immunoglobulin transport. | 2016 | 26238686 |
| conformational heterogeneity determined by folding and oligomer assembly routes of the interferon response inhibitor ns1 protein, unique to human respiratory syncytial virus. | the nonstructural ns1 protein is an essential virulence factor of the human respiratory syncytial virus, with a predominant role in the inhibition of the host antiviral innate immune response. this inhibition is mediated by multiple protein-protein interactions and involves the formation of large oligomeric complexes. there is neither a structure nor sequence or functional homologues of this protein, which points to a distinctive mechanism for blocking the interferon response among viruses. the ... | 2015 | 26237467 |
| serious adverse events in the canadian registry of children receiving palivizumab (caress) for respiratory syncytial virus prevention. | to evaluate the safety and tolerability of palivizumab for rsv prophylaxis in high-risk children in everyday practice. | 2015 | 26237402 |
| epidemiological features of bronchiolitis in the pediatric clinic of clinical center of sarajevo university. | to present the epidemiological features of bronchiolitis in a one-year period in patients of pediatric clinic, clinical centre of sarajevo university. | 2015 | 26236159 |
| a metabolomics approach to studying the effects of jinxin oral liquid on rsv-infected mice using uplc/ltq-orbitrap mass spectrometry. | jinxin oral liquid (jol) is a traditional chinese medicine (tcm) formula modified from ma-xing-shi-gan-tang, an ancient formula widely used in the treatment of respiratory diseases such as bronchitis, pneumonia, and asthma. in our previous studies, jol was shown to safely and effectively treat viral pneumonia, especially that involving respiratory syncytial virus (rsv). | 2015 | 26234176 |
| association of respiratory picornaviruses with high acuity and severe illness in a pediatric health care system. | we aimed to determine the illness severity associated with respiratory picornaviruses (rhinovirus/enterovirus). | 2015 | 26231633 |
| morphogenesis of respiratory syncytial virus in human primary nasal ciliated epithelial cells occurs at surface membrane microdomains that are distinct from cilia. | the distribution of cilia and the respiratory syncytial virus (rsv) nucleocapsid (n) protein, fusion (f) protein, attachment (g) protein, and m2-1 protein in human ciliated nasal epithelial cells was examined at between 1 and 5 days post-infection (dpi). all virus structural proteins were localized at cell surface projections that were distinct from cilia. the f protein was also trafficked into the cilia, and while its presence increased as the infection proceeded, the n protein was not detected ... | 2015 | 26231613 |
| building a better neonatal mouse model to understand infant respiratory syncytial virus disease. | respiratory syncytial virus (rsv) is the number one cause of lower respiratory tract infection in infants; and severe rsv infection in infants is associated with asthma development. today, there are still no vaccines or specific antiviral therapies against rsv. the mechanisms of rsv pathogenesis in infants remain elusive. this is partly due to the fact that the largely-used mouse model is semi-permissive for rsv. the present study sought to determine if a better neonatal mouse model of rsv infec ... | 2015 | 26231396 |
| hubs to spread technology and save lives. | the patent on an expensive preventive treatment for respiratory syncytial virus infections expires this year. a who technology transfer hub in the netherlands aims to help developing countries make the drug themselves. gary humphreys reports. | 2015 | 26229198 |
| oral ribavirin for the treatment of noninfluenza respiratory viral infections: a systematic review. | to review clinical outcomes data for patients treated with oral ribavirin for noninfluenza respiratory viral infections (nirvis). | 2015 | 26228937 |
| short- and long-term pulmonary outcome of palivizumab in children born extremely prematurely. | palivizumab reduces the severity of respiratory syncytial virus infection in premature infants, but whether there is a protective effect beyond the preschool age is unknown. this study sought to assess the short- and long-term effects of palivizumab immunization on respiratory morbidity and pulmonary function at school age in children born extremely prematurely. | 2016 | 26226546 |
| attenuated human parainfluenza virus type 1 (hpiv1) expressing the fusion glycoprotein of human respiratory syncytial virus (rsv) as a bivalent hpiv1/rsv vaccine. | live attenuated recombinant human parainfluenza virus type 1 (rhpiv1) was investigated as a vector to express the respiratory syncytial virus (rsv) fusion (f) glycoprotein, to provide a bivalent vaccine against rsv and hpiv1. the rsv f gene was engineered to include hpiv1 transcription signals and inserted individually into three gene locations in each of the two attenuated rhpiv1 backbones. each backbone contained a single previously described attenuating mutation that was stabilized against de ... | 2015 | 26223633 |
| respiratory syncytial virus infection inhibits tlr4 signaling via up-regulation of mir-26b. | respiratory syncytial virus (rsv) is the leading cause of lower respiratory tract illnesses in infants worldwide. tlr4 signal pathway plays a critical role in regulating immune response against rsv infection. however, the activation of tlr4 in rsv infection is still unclear. in present study, the expression levels of mir-26b and tlr4 mrna were detected in peripheral blood mononuclear cells (pbmcs) of children with or without rsv infected bronchiolitis. the expression levels of tlr4 and its downs ... | 2015 | 26222045 |
| does azithromycin modify viral load during severe respiratory syncytial virus bronchiolitis? | 2015 | 26215052 | |
| human respiratory syncytial virus in children with lower respiratory tract infections or influenza-like illness and its co-infection characteristics with viruses and atypical bacteria in hangzhou, china. | human respiratory syncytial virus (rsv) is the most important viral pathogen in children. however, its epidemic patterns and co-infection characteristics are not fully understood. | 2015 | 26209367 |
| enhanced immunogenicity of a respiratory syncytial virus (rsv) f subunit vaccine formulated with the adjuvant gla-se in cynomolgus macaques. | respiratory syncytial virus (rsv) causes significant disease in elderly adults, but an effective vaccine is not yet available. we have previously reported that vaccines consisting of engineered respiratory syncytial virus soluble fusion protein (rsv sf) adjuvanted with glucopyranosyl lipid a (gla) in an oil-in-water emulsion (stable emulsion [se]) induce rsv f-specific t and b cell responses in mice and rats that protect from viral challenge. here, we evaluated the immunogenicity of gla-se adjuv ... | 2015 | 26206269 |
| epidemiology and clinical outcome of lower respiratory tract infections by respiratory syncytial virus or parainfluenza virus type 3 in adults receiving treatment for either acute leukemia or severe aplastic anemia: a retrospective single center study. | 2015 | 26204825 | |
| respiratory syncytial virus-like nanoparticle vaccination induces long-term protection without pulmonary disease by modulating cytokines and t-cells partially through alveolar macrophages. | the mechanisms of protection against respiratory syncytial virus (rsv) are poorly understood. virus-like nanoparticles expressing rsv glycoproteins (eg, a combination of fusion and glycoprotein virus-like nanoparticles [fg vlps]) have been suggested to be a promising rsv vaccine candidate. to understand the roles of alveolar macrophages (ams) in inducing long-term protection, mice that were 12 months earlier vaccinated with formalin-inactivated rsv (fi-rsv) or fg vlps were treated with clodronat ... | 2015 | 26203246 |
| [detection of respiratory syncytial virus in the conjunctiva of a child with bronchiolitis caused by this virus]. | 2015 | 26200035 | |
| antiviral activities of whey proteins. | milk contains an array of proteins with useful bioactivities. many milk proteins encompassing native or chemically modified casein, lactoferrin, alpha-lactalbumin, and beta-lactoglobulin demonstrated antiviral activities. casein and alpha-lactalbumin gained anti-hiv activity after modification with 3-hydroxyphthalic anhydride. many milk proteins inhibited hiv reverse transcriptase. bovine glycolactin, angiogenin-1, lactogenin, casein, alpha-lactalbumin, beta-lactoglobulin, bovine lactoferrampin, ... | 2015 | 26198883 |
| innate and adaptive cellular phenotypes contributing to pulmonary disease in mice after respiratory syncytial virus immunization and infection. | respiratory syncytial virus (rsv) is the major leading cause of infantile viral bronchiolitis. however, cellular phenotypes contributing to the rsv protection and vaccine-enhanced disease remain largely unknown. upon rsv challenge, we analyzed phenotypes and cellularity in the lung of mice that were naïve, immunized with formalin inactivated rsv (fi-rsv), or re-infected with rsv. in comparison with naïve and live rsv re-infected mice, the high levels of eosinophils, neutrophils, plasmacytoid and ... | 2015 | 26196232 |
| gene expression profiles link respiratory viral infection, platelet response to aspirin, and acute myocardial infarction. | influenza infection is associated with myocardial infarction (mi), suggesting that respiratory viral infection may induce biologic pathways that contribute to mi. we tested the hypotheses that 1) a validated blood gene expression signature of respiratory viral infection (viral ges) was associated with mi and 2) respiratory viral exposure changes levels of a validated platelet gene expression signature (platelet ges) of platelet function in response to aspirin that is associated with mi. | 2015 | 26193668 |
| potential treatment for the respiratory syncytial virus infections (hrsv). | 2015 | 26191357 | |
| healthy late-preterm infants born 33-36+6 weeks gestational age have higher risk for respiratory syncytial virus hospitalization. | respiratory syncytial virus (rsv) is a leading cause of hospitalization for children <1year old and is more severe in premature infants. | 2015 | 26186560 |
| low eosinophils during bronchiolitis in infancy are associated with lower risk of adulthood asthma. | infant bronchiolitis may be the first manifestation of asthma. | 2015 | 26186154 |
| leptin is oversecreted by respiratory syncytial virus-infected bronchial epithelial cells and regulates th2 and th17 cell differentiation. | infection of human bronchial epithelial cells (hbecs) with respiratory syncytial virus (rsv) has been shown to induce a th lymphocyte subset drift, e.g. enhanced differentiation of th2 and th17 subsets, which is a classic characteristic of asthma. however, the molecules responsible for the drift in th subsets remain unknown. this study aims to determine the expression of leptin in rsv-infected hbecs, and its role in th2 and th17 cell differentiation and extracellular regulated kinase (erk) 1/2 p ... | 2015 | 26184438 |
| respiratory viral detection in children and adults: comparing asymptomatic controls and patients with community-acquired pneumonia. | the clinical significance of viruses detected in patients with community-acquired pneumonia (cap) is often unclear. | 2016 | 26180044 |
| 2'-5'-oligoadenylate synthetase-like protein inhibits respiratory syncytial virus replication and is targeted by the viral nonstructural protein 1. | 2'-5'-oligoadenylate synthetase-like protein (oasl) is an interferon-inducible antiviral protein. here we describe differential inhibitory activities of human oasl and the two mouse oasl homologs against respiratory syncytial virus (rsv) replication. interestingly, nonstructural protein 1 (ns1) of rsv promoted proteasome-dependent degradation of specific oasl isoforms. we conclude that oasl acts as a cellular antiviral protein and that rsv ns1 suppresses this function to evade cellular innate im ... | 2015 | 26178980 |
| animal models of respiratory syncytial virus infection and disease. | the study of human respiratory syncytial virus pathogenesis and immunity has been hampered by its exquisite host specificity, and the difficulties encountered in adapting this virus to a murine host. the reasons for this obstacle are not well understood, but appear to reflect, at least in part, the inability of the virus to block the interferon response in any but the human host. this review addresses some of the issues encountered in mouse models of respiratory syncytial virus infection, and de ... | 2015 | 26176495 |
| risk of urinary tract infection in infants and children with acute bronchiolitis. | to estimate the prevalence of urinary tract infection in infants and children with bronchiolitis. | 2017 | 26175566 |
| respiratory syncytial virus is present in the neonatal intensive care unit. | nosocomial transmission of respiratory syncytial virus (rsv) occurs in children within the neonatal intensive care unit (nicu). during peak community rsv transmission, three swabs were collected from the nose, hand and personal clothing of visitors and health care workers (hcw) in nicu once every week for eight weeks. nasal swabs were collected from every third neonate and from any neonate clinically suspected of having a respiratory infection. environmental sampling of high touch areas was done ... | 2016 | 26174559 |
| mammalian cell-derived respiratory syncytial virus-like particles protect the lower as well as the upper respiratory tract. | globally, respiratory syncytial virus (rsv) is a leading cause of bronchiolitis and pneumonia in children less than one year of age and in usa alone, between 85,000 and 144,000 infants are hospitalized every year. to date, there is no licensed vaccine. we have evaluated vaccine potential of mammalian cell-derived native rsv virus-like particles (rsv vlps) composed of the two surface glycoproteins g and f, and the matrix protein m. results of in vitro testing showed that the vlps were functionall ... | 2015 | 26172453 |
| influence of respiratory viruses on the evaluation of the 13-valent pneumococcal conjugate vaccine effectiveness in children under 5 years old: a time-series study for the 2001-2013 period. | s treptococcus pneumoniae is the main agent in bacterial consolidated pneumonias. in 2012, the 13-valent pneumococcal conjugate vaccine was introduced in the argentine national immunization schedule for immunocompetent children as of two months old with a two-dose schedule plus a booster. | 2015 | 26172005 |
| [prevalence and seasonal distribution of respiratory viruses in patients with acute respiratory tract infections, 2002-2014]. | the aim of this study was to investigate the prevalence and seasonal distribution of respiratory viruses in pediatric and adult outpatients and inpatients who were admitted to hospital with the symptoms of upper and lower respiratory tract infections, during a 12-year period. a total of 5102 clinical samples (4372 nasopharyngeal swabs, 316 bronchoalveolar lavages, 219 transtracheal aspirates, 163 nasopharyngeal aspirates, 20 sputum, 10 nasal swabs) examined in our laboratory between january 1st ... | 2015 | 26167819 |
| [research progress in the f gene and protein of the respiratory syncytial virus]. | the respiratory syncytial virus (rsv) belongs to the family paramyxoviridae and subfamily pneumovirinae. the rsv can cause acute infections of the lower respiratory tract in infants. the f gene of the rsv is a conservative gene and varies only slightly in its expression. few studies focusing on the variability of the f gene have been carried out. f protein (fusion glycoprotein) is a transmembrane glycoprotein that mediates fusion and penetration between the virus and host cells. neutralizing ant ... | 2015 | 26164949 |
| role of nasopharyngeal bacteria and respiratory viruses in acute symptoms of young children. | the spectrum of acute symptoms in young outpatient children with respiratory tract infection (rti) is variable, and it cannot be explained by the diagnosis of acute otitis media (aom) versus uncomplicated rti. we studied that the variation of symptoms is explained by the nasopharyngeal bacteria and/or respiratory viruses. | 2015 | 26164848 |
| olfactomedin 4 serves as a marker for disease severity in pediatric respiratory syncytial virus (rsv) infection. | respiratory viral infections follow an unpredictable clinical course in young children ranging from a common cold to respiratory failure. the transition from mild to severe disease occurs rapidly and is difficult to predict. the pathophysiology underlying disease severity has remained elusive. there is an urgent need to better understand the immune response in this disease to come up with biomarkers that may aid clinical decision making. | 2015 | 26162090 |
| characterization of a prefusion-specific antibody that recognizes a quaternary, cleavage-dependent epitope on the rsv fusion glycoprotein. | prevention efforts for respiratory syncytial virus (rsv) have been advanced due to the recent isolation and characterization of antibodies that specifically recognize the prefusion conformation of the rsv fusion (f) glycoprotein. these potently neutralizing antibodies are in clinical development for passive prophylaxis and have also aided the design of vaccine antigens that display prefusion-specific epitopes. to date, prefusion-specific antibodies have been shown to target two antigenic sites o ... | 2015 | 26161532 |
| sirtuin 1 regulates dendritic cell activation and autophagy during respiratory syncytial virus-induced immune responses. | respiratory syncytial virus (rsv) is the major cause of lower respiratory tract infection in children worldwide. sirtuin 1 (sirt1), an nad(+)-dependent deacetylase, has been associated with the induction of autophagy and the regulation of inflammatory mediators. we found that sirt1 was upregulated in mouse lung after rsv infection. infected animals that received ex-527, a selective sirt1 inhibitor, displayed exacerbated lung pathology, with increased mucus production, elevated viral load, and en ... | 2015 | 26157176 |
| enhanced neutralizing antibody response induced by respiratory syncytial virus prefusion f protein expressed by a vaccine candidate. | respiratory syncytial virus (rsv) and human parainfluenza virus type 3 (hpiv3) are the first and second leading viral agents of severe respiratory tract disease in infants and young children worldwide. vaccines are not available, and an rsv vaccine is particularly needed. a live attenuated chimeric recombinant bovine/human piv3 (rb/hpiv3) vector expressing the rsv fusion (f) glycoprotein from an added gene has been under development as a bivalent vaccine against rsv and hpiv3. previous clinical ... | 2015 | 26157122 |
| respiratory syncytial virus utilizes a trna fragment to suppress antiviral responses through a novel targeting mechanism. | target identification is highly instructive in defining the biological roles of micrornas. however, little is known about other small noncoding rnas; for example, trna-derived rna fragments (trfs). some trfs exhibit a gene-silencing mechanism distinctly different from that of typical micrornas. we recently demonstrated that a respiratory syncytial virus (rsv)-induced trf, called trf5-gluctc, promotes rsv replication. rsv is the single most important cause of lower respiratory tract infection in ... | 2015 | 26156244 |
| cell-contact dependent inhibition of monocytes by airway epithelial cells and reversion by infection with respiratory syncytial virus. | airway epithelial cells (aec) are the first line of defense against airborne infectious microbes and play an important role in regulating the local immune response. however, the interplay of epithelial cells and professional immune cells during both homeostasis and infection has only been partially studied. the present study was performed to determine how bronchial epithelial cells affect the activation of monocytes. under healthy conditions, aecs were shown to inhibit reactivity of monocytes. w ... | 2015 | 26153873 |
| respiratory syncytial virus infections in pediatric transplant recipients: a canadian paediatric surveillance program study. | the incidence and spectrum of severity of rsv infections in sot or hsct recipients is not known. from september 2010 through august 2013, pediatricians were surveyed monthly by the cpsp for sot or hsct recipients with rsv infection within two yr post-transplant. there were 24 completed case report forms that fit the inclusion criteria (10 hsct and 14 sot recipients). six of 24 cases (25%) remained outpatients, and 11 (46%) were managed on an inpatient ward, while seven (29%) required intensive c ... | 2015 | 26152857 |
| human respiratory syncytial virus and th1 chemokines. | the importance of the type-1 helper (th1) chemokines in rsv (respiratory syncytial virus) infection has been shown by several studies, in animal models, and in humans. the production of th1 chemokines within the lower respiratory tract has been shown in patients with rsv bronchiolitis, particularly chemokine (c-x-c motif) ligand (cxcl)10/interferon-γ-inducible protein 10 (ip-10), whose plasma levels were markedly elevated in acute infection. rsv fusion (f) or large (g) protein treatment of fully ... | 2015 | 26152633 |
| urban particulate matter increases human airway epithelial cell il-1β secretion following scratch wounding and h1n1 influenza a exposure in vitro. | the airway epithelium represents the first line of defense against inhaled environmental insults including air pollution, allergens, and viruses. epidemiological and experimental evidence has suggested a link between air pollution exposure and the symptoms associated with respiratory viral infections. we hypothesized that multiple insults integrated by the airway epithelium nlrp3 inflammasome would result in augmented il-1β release and downstream cytokine production following respiratory virus e ... | 2015 | 26151556 |
| nasopharyngeal bacterial burden and antibiotics: influence on inflammatory markers and disease severity in infants with respiratory syncytial virus bronchiolitis. | animal studies suggest that rsv increases nasopharyngeal (np) bacterial colonization facilitating bacterial infections. we investigated the influence of antibiotic treatment and colonization with potentially pathogenic bacteria on inflammatory markers and disease severity in rsv-infected in infants. | 2015 | 26149186 |
| clinical and epidemiological characteristics of acute respiratory virus infections in vietnamese children. | information about viral acute respiratory infections (aris) is essential for prevention, diagnosis and treatment, but it is limited in tropical developing countries. this study described the clinical and epidemiological characteristics of aris in children hospitalized in vietnam. nasopharyngeal samples were collected from children with aris at ho chi minh city children's hospital 2 between april 2010 and may 2011 in order to detect respiratory viruses by polymerase chain reaction. viruses were f ... | 2016 | 26145204 |
| a comparison of human metapneumovirus and respiratory syncytial virus who-defined severe pneumonia in moroccan children. | acute respiratory infections remain the principal cause of morbidity and mortality in moroccan children. besides bacterial infections, respiratory syncytial virus (rsv) and human metapneumovirus (hmpv) are prominent among other viruses due to their high prevalence and association with severe clinical episodes. we aimed to describe and compare rsv- and hmpv-associated cases of who-defined severe pneumonia in a paediatric population admitted to morocco's reference hospital. children aged 2-59 mont ... | 2016 | 26143933 |
| estimating the contribution of influenza to hospitalisations in new zealand from 1994 to 2008. | influenza has a substantially but poorly measured impact on population health. estimating its true contribution to hospitalisations remains a challenge. | 2015 | 26143611 |
| prevalence of human respiratory syncytial virus circulating in iran. | respiratory syncytial virus (rsv) is a leading cause of acute respiratory infection during early childhood and is associated with a great burden on patients, parents, and society. while no treatment is yet available, results from recent phase 2 clinical trials of cell-entry inhibitors and rsv vaccines are promising. to prepare for introduction of these novel therapeutics, good understanding of its molecular epidemiology and continuous rsv surveillance data are necessary. this paper provides an o ... | 2016 | 26143136 |
| evaluation of a new rapid diagnostic test for the detection of influenza and rsv. | influenza viruses and respiratory syncytial virus (rsv) can cause an acute respiratory disease that occurs seasonally in epidemic waves. this retrospective study was conducted to evaluate the sofia(®) influenza a+b and the sofia(®) rsv fluorescence immunoassays (fias), two novel rapid detection tests (rdts) for influenza a and b and rsv. | 2016 | 26139303 |
| google flu trends in canada: a comparison of digital disease surveillance data with physician consultations and respiratory virus surveillance data, 2010-2014. | the value of google flu trends (gft) remains unclear after it overestimated the proportion of physician visits related to influenza-like illness (ili) in the united states in 2012-2013. however, gft estimates (%gft) have not been examined nationally in canada nor compared with positivity for respiratory viruses other than influenza. for 2010-2014, we compared %gft for canada to public health agency of canada ili consultation rates (%phac) and to positivity for influenza a and b, respiratory sync ... | 2016 | 26135239 |
| viral load in infants hospitalized for respiratory syncytial virus bronchiolitis correlates with recurrent wheezing at thirty-six-month follow-up. | the relationship between viral infection, host immune response in infants with respiratory syncytial virus (rsv) bronchiolitis and subsequent wheezing is discussed. we measured rsv-rna load and interferon-λ1-3 expression in the nasopharyngeal washings from 68 infants hospitalized for rsv bronchiolitis, and wheezing was assessed 36 months after the first episode of bronchiolitis. higher rsv-rna load and higher interferon-λ2/3 levels were found in children with recurrent wheezing at 36-month follo ... | 2015 | 26132826 |
| effect of prophylactic palivizumab on admission due to respiratory syncytial virus infection in former very low birth weight infants with bronchopulmonary dysplasia. | the aim of this study was to observe the effects of prophylactic palivizumab on hospitalization secondary to respiratory syncytial virus (rsv) infection (rsvhospitalization) in former very low birth weight infants (vlbwi) with bronchopulmonary dysplasia (bpd). this study also sought to identify the risk factors of rsvhospitalizationin this particular infant population. a prospective observational study was conducted between september 2007 and april 2008 in seven korean hospitals. children with a ... | 2015 | 26130956 |
| a respiratory syncytial virus persistent-infected cell line system reveals the involvement of socs1 in the innate antiviral response. | hep-2 cells persistently infected with respiratory syncytial virus (rsv) are a heterogeneous mixture of viral antigen-positive and -negative variants; however, the mechanism through which viral replication becomes latent remains unclear. in this study, we investigated the potential mechanism by which rsv escapes from innate immune surveillance. persistent-infected rsv hep-2 cells were isolated and cell clones were passaged. the rsv-persistent cells produced viruses at a lower titer, resisted wil ... | 2015 | 26122642 |
| immunological response to single pathogen challenge with agents of the bovine respiratory disease complex: an rna-sequence analysis of the bronchial lymph node transcriptome. | susceptibility to bovine respiratory disease (brd) is multi-factorial and is influenced by stress in conjunction with infection by both bacterial and viral pathogens. while vaccination is broadly used in an effort to prevent brd, it is far from being fully protective and cases diagnosed from a combination of observed clinical signs without any attempt at identifying the causal pathogens are usually treated with antibiotics. dairy and beef cattle losses from brd are profound worldwide and genetic ... | 2015 | 26121276 |
| respiratory viral detections during symptomatic and asymptomatic periods in young andean children. | viruses are commonly detected in children with acute respiratory illnesses (aris) and in asymptomatic children. longitudinal studies of viral detections during asymptomatic periods surrounding ari could facilitate interpretation of viral detections but are currently scant. | 2015 | 26121205 |
| clinical endpoints for respiratory syncytial virus prophylaxis trials in infants and children in high-income and middle-income countries. | respiratory syncytial virus (rsv) continues to cause significant clinical and economic burden around the world. historically, rsv-associated hospitalization was used as a primary endpoint for rsv prophylaxis trials in infants. however, because of the changing epidemiology and healthcare system landscape, this endpoint has become a critical bottleneck on the pathway to licensure for new therapeutics. a panel of 7 rsv experts was convened (chicago, il, may 22, 2014) to evaluate the challenges of d ... | 2015 | 26121204 |
| detection of multiple respiratory viruses associated with mortality and severity of illness in children. | respiratory viral infection is a common source of morbidity and mortality in children. coinfection with multiple viruses occurs frequently; however, the clinical significance of concomitant viral pathogens is unclear. we hypothesized that presence of more than one respiratory virus is associated with increased morbidity and mortality when compared with children with a single respiratory virus. | 2015 | 26121097 |
| shp-2 contributes to anti-rsv activity in human pulmonary alveolar epithelial cells by interfering with the ifn-α-induced jak/stat1 pathway. | src homology phosphotyrosyl phosphatase 2 (shp-2) is a ubiquitously expressed protein that is involved in a variety of cellular processes, including antiviral interferon signalling pathways. in this study, we investigated the role of shp-2 in the host cell interactions of human respiratory syncytial virus (rsv). we report significant changes in the expression of shp-2 in human pulmonary alveolar epithelial cells (a549) upon rsv infection. we also report that blocking shp-2 does not affect viral ... | 2015 | 26119280 |
| new insights on the viral and host factors contributing to the airway pathogenesis caused by the respiratory syncytial virus. | the respiratory syncytial virus (rsv) is the most prevalent etiological agent of lower respiratory tract infections and the first cause of hospitalization in infants due to respiratory disease worldwide. however, efforts to develop safe and effective vaccines and antivirals have been challenged by an incomplete understanding of the rsv pathogenesis and the host immune response to rsv infection in the airways. here, we discuss recent advances in understanding the interaction between rsv and the e ... | 2016 | 26119025 |
| significant transplantation-related mortality from respiratory virus infections within the first one hundred days in children after hematopoietic stem cell transplantation. | respiratory viral infections (rvi) are important in hematopoietic stem cell transplantations (hsct) and knowledge regarding incidence, morbidity, mortality, and long-term pulmonary complications is limited. we report a study to evaluate incidence and outcomes, both short and long-term, of rvi in children receiving hsct. between january 2000 and december 2012, 844 patients underwent hematopoietic stem cell transplantation (hsct) at the hospital for sick children: 491 were allogeneic and 353 were ... | 2015 | 26117558 |
| human metapneumovirus infection is associated with severe respiratory disease in preschool children with history of prematurity. | human metapneumovirus (hmpv) is a recently discovered respiratory pathogen of the family paramyxoviridae, the same family as that of respiratory syncytial virus (rsv). premature children are at high risk of severe rsv infections, however, it is unclear whether hmpv infection is more severe in hospitalized children with a history of severe prematurity. | 2016 | 26117550 |
| benzimidazole analogs inhibit respiratory syncytial virus g protein function. | human respiratory syncytial virus (hrsv) is a highly contagious paramyxovirus that infects most children by age two, generating an estimated 75,000-125,000 hospitalizations in the u.s. annually. hrsv is the most common cause of bronchiolitis and pneumonia among infants and children under 1year of age, with significant mortality among high-risk groups. a regulatory agency-approved vaccine is not available, and existing prophylaxis and therapies are limited to use in high-risk pediatric patients; ... | 2015 | 26116756 |
| increasing complexity of vaccine development. | vaccines already developed have been enormously successful. however, the development of future vaccines requires solution of a number of immunologic problems, including pathogen variability, short effector memory, evoking functional responses, and identification of antigens that generate protective responses. in addition, different populations may respond differently to the same vaccine because of genetic, age, or environmental factors. | 2015 | 26116724 |
| a recombinant varicella vaccine harboring a respiratory syncytial virus gene induces humoral immunity. | the varicella-zoster virus (vzv) oka vaccine strain (voka) is highly efficient and causes few adverse events; therefore, it is used worldwide. we previously constructed recombinant voka (rvoka) harboring the mumps virus gene. immunizing guinea pigs with rvoka induced the production of neutralizing antibodies against the mumps virus and vzv. here, we constructed recombinant voka viruses containing either the respiratory syncytial virus (rsv) subgroup a fusion glycoprotein (rsv a-f) gene or rsv su ... | 2015 | 26116253 |
| urokinase receptor-deficient mice mount an innate immune response to and clarify respiratory viruses as efficiently as wild-type mice. | the plasminogen activator receptor (upar) is required for lung infiltration by innate immune cells in respiratory bacterial infections. in order to verify if this held true for respiratory viruses, wild type (wt) and upar knockout (upar(-/-)) mice were inoculated intranasally with the human respiratory syncytial virus (hrsv) and the influenza a virus. at several days post-infection (dpi), viral titers in the lungs were determined while cell infiltrates in the bronchoalveolar lavage (bal) were an ... | 2015 | 26115163 |
| human rhinovirus infections in hospitalized children: clinical, epidemiological and virological features. | molecular epidemiology and clinical impact of human rhinovirus (hrv) are not well documented in tropical regions. this study compared the clinical characteristics of hrv to other common viral infections and investigated the molecular epidemiology of hrv in hospitalized children with acute respiratory infections (aris) in vietnam. from april 2010 to may 2011, 1082 nasopharyngeal swabs were screened for respiratory viruses by pcr. vp4/vp2 sequences of hrv were further characterized. hrv was the mo ... | 2016 | 26112743 |
| early evolution of human respiratory syncytial virus on1 strains: analysis of the diversity in the c-terminal hypervariable region of glycoprotein gene within the first 3.5 years since their detection. | characterization of the phylogeny and diversity of human respiratory syncytial virus (hrsv) genotype on1 that occurred during its early evolution (within the first 3.5 years since the detection of the first on1 strains). on1 strains have a 72-nucleotide-long in-frame duplication within the second hypervariable domain of the glycoprotein gene (hvr2). | 2015 | 26112390 |
| incidence and risk factors for respiratory syncytial virus and human metapneumovirus infections among children in the remote highlands of peru. | the disease burden and risk factors for respiratory syncytial virus (rsv) and human metapneumovirus (mpv) infections among children living in remote, rural areas remain unclear. | 2015 | 26107630 |
| recombinant soluble respiratory syncytial virus f protein that lacks heptad repeat b, contains a gcn4 trimerization motif and is not cleaved displays prefusion-like characteristics. | the respiratory syncytial virus (rsv) fusion protein f is considered an attractive vaccine candidate especially in its prefusion conformation. we studied whether recombinant soluble rsv f proteins could be stabilized in a prefusion-like conformation by mutation of heptad repeat b (hrb). the results show that soluble, trimeric, non-cleaved rsv f protein, produced by expression of the furin cleavage site-mutated f ectodomain extended with a gcn4 trimerization sequence, is efficiently recognized by ... | 2015 | 26107504 |
| relating plaque morphology to respiratory syncytial virus subgroup, viral load, and disease severity in children. | viral culture plaque morphology in human cell lines are markers for growth capability and cytopathic effect, and have been used to assess viral fitness and select preattenuation candidates for live viral vaccines. we classified respiratory syncytial virus (rsv) plaque morphology and analyzed the relationship between plaque morphology as compared to subgroup, viral load and clinical severity of infection in infants and children. | 2015 | 26107392 |
| cx3cr1 is expressed in differentiated human ciliated airway cells and co-localizes with respiratory syncytial virus on cilia in a g protein-dependent manner. | respiratory syncytial virus (rsv) is the principal cause of bronchiolitis in infants and a significant healthcare problem. the rsv glycoprotein (g) mediates attachment of the virus to the cell membrane, which facilitates interaction of the rsv fusion (f) protein with nucleolin, thereby triggering fusion of the viral and cellular membranes. however, a host protein ligand for g has not yet been identified. here we show that cx3cr1 is expressed in the motile cilia of differentiated human airway epi ... | 2015 | 26107373 |