Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| lobar collapse with respiratory syncytial virus pneumonitis. | in a study of 30 children with uncomplicated respiratory syncytial virus (rsv) pneumonias, a high incidence of lobar collapse (8/30-26%) was noted. this involved the right upper lobe in seven patients and the left upper lobe in one patient. it is probably attributable to anatomical predispositions, sloughing of necrotic epithelium, and stimulation of mucus production. lobar collapse should be considered part of the spectrum of rsv pneumonitis. | 1985 | 4000751 |
| respiratory syncytial virus-specific ige responses following infection: evidence for a predominantly mucosal response. | in order to determine whether ige production occurs predominantly at mucosal or systemic sites, we studied the production of respiratory syncytial virus (rsv)-specific antibody in serum and nasopharyngeal secretions (nps) from 41 patients with rsv infection using an enzyme-linked immunosorbent assay. rsv-ige was found in higher titer in samples of nps than in simultaneously obtained serum specimens at all phases of illness. despite the excess dilution incurred in the collection process, rsv-ige ... | 1985 | 4000767 |
| severe respiratory syncytial virus infection: clinical features, nosocomial acquisition and outcome. | respiratory syncytial virus (rsv) frequently causes bronchiolitis in young infants. rsv infection was established by culture, serologic response, immunofluorescence of secretions or lung tissue or typical histology on pathologic examination of the lungs in 10 patients who were hospitalized at children's medical center, dallas. eight were infants 4 days to 3 months old, one was 9 months and one was 16 months old. seven patients were afebrile but had rapidly progressive diffuse infiltrates on ches ... | 1985 | 4000987 |
| respiratory syncytial virus polypeptides. v. the kinetics of glycoprotein synthesis. | the cell-associated glycoproteins of respiratory syncytial virus included gp1 (90k), vp70 (70k), vgp48 (48k) and gp26 (26k). the time course of virus protein synthesis in hela cells revealed that the nucleocapsid protein (vpn41) was the first to appear at 11 h post-infection followed by the appearance of the other viral proteins at 16 h post-infection. pulse-chase experiments with [3h]leucine or [35s]methionine demonstrated that the precursor for vgp48 and gp26 was an uncleaved protein of 70k (v ... | 1985 | 4009147 |
| mrna sequence of three respiratory syncytial virus genes encoding two nonstructural proteins and a 22k structural protein. | an mrna sequence of two human respiratory syncytial viral nonstructural protein genes and of a gene for a 22,000-molecular-weight (22k) protein was obtained by cdna cloning and dna sequencing. sequences corresponding to the 5' ends of the respective transcripts were deduced directly by primer extension and dideoxy nucleotide sequencing of the mrnas. the availability of a bicistronic clone (prsc6) confirmed the gene order for this portion of the genome. contrary to other unsegmented negative-stra ... | 1985 | 4009789 |
| comparison of a newly developed enzyme-linked immunosorbent assay with complement fixation and neutralisation tests for serology of bovine respiratory syncytial virus infections. | an indirect double antibody sandwich enzyme-linked immunosorbent assay (elisa) was developed for the detection and titration of serum antibodies to bovine respiratory syncytial virus (brsv). the elisa was compared with a complement fixation (cf) test and a test for virus neutralising antibody in serum (virus neutralisation [vn] test). testing sera collected in dairy herds revealed the closest correlation between the results of the elisa and the cf test with respect to brsv antibody titres. the v ... | 1985 | 4012035 |
| an outbreak of respiratory syncytial virus infection in a continuing-care geriatric ward. | a small outbreak of respiratory syncytial virus infection in a continuing-care geriatric ward during the period december 1982 to march 1983 is reported. it demonstrates that respiratory syncytial virus infection can cause serious lower respiratory tract infection even in patients without any previous history of chest disease. | 1985 | 4013906 |
| respiratory tract infections. goals for 1995. | goals to be identified for 1995, a decade hence, in the prevention, diagnosis, and management of respiratory tract infections may conveniently be divided into diagnostic goals and goals in therapy and prophylaxis. major diagnostic goals for bacterial, viral, and mycoplasmal infections of the respiratory tract focus on the development of systems to identify microbial components, such as specific antigens or segments of dna, using monoclonal antibody techniques or dna probes for hybridization. spu ... | 1985 | 4014289 |
| serologic studies of bovine respiratory syncytial virus in minnesota cattle. | serum antibody titers to bovine respiratory syncytial virus were determined for 559 cattle. serum samples were obtained through the minnesota state-federal brucellosis laboratory and were collected over a 1-year period. results of this study revealed an antibody prevalence of 65.5% to bovine respiratory syncytial virus. the distribution of antibody titers is presented, as well as analysis of titers based on breed, sex, and age of the cattle. | 1985 | 4014838 |
| etiology of community-acquired pneumonia in patients requiring hospitalization. | the etiology of community-acquired pneumonia was studied in 127 patients with roentgenologically verified pneumonia who needed hospitalization. etiology was determined on the basis of a positive blood culture and/or a significant antibody titer increase. streptococcus pneumoniae was the probable etiological agent in 69 patients, nontypeable haemophilus influenzae in five patients, streptococcus pyogenes in two patients, and legionella pneumophila and staphylococcus aureus in one patient each. ev ... | 1985 | 4018066 |
| quantitative aspects of passive immunity to respiratory syncytial virus infection in infant cotton rats. | the amount of passively acquired serum respiratory syncytial virus (rsv)-neutralizing antibodies required to protect the respiratory tract of cotton rats against infection was studied. infant cotton rats were inoculated intraperitoneally with various dilutions of a single pool of sera derived from cotton rats convalescent from rsv infection. after 24 h, these animals were inoculated with rsv intranasally. virus replication in the respiratory tract was suppressed in cotton rats which had a serum ... | 1985 | 4020957 |
| [incidence of respiratory syncytial virus infection in case records of a pediatric general medicine department]. | 1985 | 4021934 | |
| kinetics of synthesis of respiratory syncytial virus glycoproteins. | the synthesis of the two respiratory syncytial (rs) virus glycoproteins (vp66 and vp84) was examined under standard conditions and after treatment with tunicamycin and monensin. the protein backbone for vp66, the fusion protein (f1,2) is cotranslationally glycosylated to form f0, which is cleaved to form f1,2 by 20 min of chase. monensin treatment inhibited the cleavage of f0 over an 80 min chase period, indicating that this occurred late in the transit of f0 through the golgi apparatus or after ... | 1985 | 4031826 |
| the childhood health effects of an improved water supply system on a remote panamanian island. | the incidence of diarrhea, respiratory disease, and skin infections was prospectively determined after the introduction of a system which distributed unlimited quantities of high quality fresh water to each of the 150 housing units on tupile, an island devoid of fresh water located off panama's caribbean coast and inhabited by 1,500 cuna indians. tupile residents used 7.1 liters of water/person/day compared to the 2.3 usage rate of inhabitants on achutupo, the control island. despite ready avail ... | 1985 | 4037183 |
| outbreak of respiratory syncytial virus in france. | a report is given of an outbreak of respiratory syncytial virus infection in a neonatal unit in france. twenty-three of 32 infants were infected (72%) despite infection control procedures. prophylactic administration of non-specific gamma globulins was associated with a significant decrease in infection rate (p less than 0.05). the administration of transfer factor to infected infants was also associated with a significantly lower rate of severe respiratory diseases (p less than 0.05). | 1985 | 4043060 |
| studies of passive immunotherapy for infections of respiratory syncytial virus in the respiratory tract of a primate model. | 1985 | 4045247 | |
| cellular reactivity to respiratory syncytial virus in human colostrum and breast milk. | colostrum and breast-milk samples were taken from 23 mothers between 2 days and 7 weeks postpartum and were examined for the presence of cellular reactivity to respiratory syncytial (rs) virus using a lymphocyte transformation assay. positive responses were detected in nine of the 23 (39%) samples taken at 2-5 days postpartum, but this reactivity was undetectable at 3 weeks. positive responses developed in a further three mothers during the 3-7-week period of lactation, suggesting a response to ... | 1985 | 4045438 |
| severe respiratory disease in dairy cattle in new york state associated with bovine respiratory syncytial virus infection. | severe respiratory disease associated with bovine respiratory syncytial virus (brsv) infection has been identified in dairy cattle in new york state. the cases identified occurred in dairy calves and heifers. the disease was characterized in 4 animals by pathologic changes including interstitial pneumonia, necrotizing bronchiolitis with multinucleated syncytial epithelial cells and interstitial emphysema. brsv antigen was demonstrated in lung samples or was isolated in tissue culture in all 4 ca ... | 1985 | 4053607 |
| an epizootic of respiratory syncytial virus infection in a dairy herd. | respiratory syncytial virus was the cause of a severe epizootic of bovine respiratory tract disease. the virus, isolated from a sick cow during the epizootic, produced cytopathic effect in a bovine turbinate cell line 14 days after it was inoculated. additional support for the diagnosis came from the results of pathologic and serologic examinations. lesions consistently present were severe necrotizing bronchiolitis and epithelial syncytia projecting from bronchiolar and alveolar walls. also, in ... | 1985 | 4055489 |
| severe rsv bronchiolitis in an immunocompromised child. | a 9-year-old with immunodeficiency developed a severe, diffuse respiratory illness that necessitated mechanical ventilation. open lung biopsy revealed respiratory syncytial virus (rsv) as the sole pathogen. rsv detection should be included in the differential diagnosis of diffuse lung disease in an immunocompromised child. | 1985 | 4064461 |
| microneutralization test for respiratory syncytial virus based on an enzyme immunoassay. | virus infectivity and antibody neutralization titers for respiratory syncytial virus were determined in cell cultures in microtiter plates. after an appropriate incubation period, the cells were fixed, and an enzyme-linked immunosorbent assay was performed directly in the microtiter plates for detection of virus. results could be read and recorded automatically, which is especially helpful when running large numbers of tests. | 1985 | 4066916 |
| comparison of ortho respiratory syncytial virus enzyme-linked immunosorbent assay and hep-2 cell culture. | two hundred seventy nasopharyngeal aspirates were tested in duplicate with the ortho diagnostics, inc. (raritan, n.j.), respiratory syncytial virus antigen enzyme-linked immunosorbent assay. the test was sensitive (80 to 82%) and specific (96%) when compared with cell culture. the enzyme-linked immunosorbent assay detected seven antigen-positive specimens not among the 71 specimens that were positive for respiratory syncytial virus in cell culture. a blocking test confirmed those specimens as tr ... | 1985 | 4066920 |
| bronchodilator response during acute viral bronchiolitis in infancy. | bronchodilator responsiveness was assessed by measuring specific respiratory conductance before and after inhalation of aerosolized bronchodilator in 50 infants who had acute bronchiolitis due to respiratory syncytial virus infection. thirty per cent of the infants showed an improvement in specific conductance. responders could not be differentiated from nonresponders by family histories of atopy, eosinophil counts, or immunoglobulin levels in blood and nasal secretions. eighty-three per cent of ... | 1985 | 4069797 |
| respiratory syncytial virus envelope glycoprotein (g) has a novel structure. | amino acid sequence of human respiratory syncytial virus envelope glycoprotein (g) was deduced from the dna sequence of a recombinant plasmid and confirmed by limited amino acid microsequencing of purified 90k g protein. the calculated molecular mass of the protein encoded by the only long open reading frame of 298 amino acids was 32,588 daltons and was somewhat smaller than the 36k polypeptide translated in vitro from mrna selected by this plasmid. inspection of the sequence revealed a single h ... | 1985 | 4069997 |
| [bovine respiratory syncytial virus as a pathogen in the respiratory tract diseases of cattle: a clinical and seroepidemiologic study in switzerland]. | 1985 | 4071019 | |
| [bovine respiratory syncytial virus (brsv) in switzerland: a serologic study]. | 1985 | 4071020 | |
| [infections with respiratory syncytial virus in infants and young children]. | 1985 | 4080005 | |
| [respiratory syncytial virus: rapid detection of antigen using monoclonal antibodies]. | 1985 | 4080006 | |
| respiratory syncytial virus pneumonia in friesian calves: physiological findings. | clinical, serological and physiological values were measured during and after naturally occurring respiratory syncytial virus pneumonia in four friesian calves weighing between 120 and 160 kg. clinical and serological findings were in agreement with the description of spontaneous calf pneumonia, associated with respiratory syncytial virus infection. pulmonary function values, recorded one, eight, 15 and 57 days after the clinical onset of the disease, provided good evidence that the clinical syn ... | 1985 | 4081337 |
| inhibitory effects of several antiviral compounds on the replication of respiratory syncytial virus in vitro. | 1985 | 4083870 | |
| spontaneous bovine respiratory syncytial virus infections in calves: arterial blood gas, ph and bicarbonate values. | arterial blood gas values of 12 calves in the acute stage of a bovine respiratory syncytial virus infection were compared with corresponding values of 11 healthy calves. the patients appeared to be severely hypoxic. mean (+/- sd) arterial oxygen tension (pao2) values of patients and healthy calves were 7.7 +/- 1.9 and 14.2 +/- 1.5 kpa, respectively. hypercapnia was present in only three of the diseased calves. these findings are in close agreement with findings in young children with respiratory ... | 1985 | 4090240 |
| purification and characterization of the respiratory syncytial virus fusion protein. | the fusion protein of respiratory syncytial virus was purified by affinity chromatography using a monoclonal antibody. under various conditions the protein was recovered as a 145k dimer or a 70k monomer. the 70k monomer was composed of disulphide-linked fragments of 48k and 23k. polyclonal rabbit serum produced to the dimerized fusion protein neutralized virus but did not inhibit fusion, while rabbit serum to the 2-mercaptoethanol-treated dimerized protein neutralized virus and inhibited fusion ... | 1985 | 3838336 |
| the envelope-associated 22k protein of human respiratory syncytial virus: nucleotide sequence of the mrna and a related polytranscript. | we recently determined that respiratory syncytial virus (strain a2) encodes a fourth unique envelope-associated virion protein that has molecular weight of approximately 24,000, as estimated by gel electrophoresis. the nucleotide sequence of the mrna encoding this novel protein has now been determined from five cdna clones, including three that contain the complete mrna sequence. the complete mrna sequence is 957 nucleotides, exclusive of polyadenylate, and contains two partially overlapping ope ... | 1985 | 3838351 |
| giant cell pneumonia due to respiratory syncytial virus. | 1985 | 3838449 | |
| nucleotide sequence of the respiratory syncytial virus phosphoprotein gene. | a cdna library was prepared from poly(a)+ rna extracted from respiratory syncytial (rs) virus-infected hep-2 cells. a recombinant plasmid, prsp68, encoding the rs virus phosphoprotein gene was identified by translation in vitro of hybrid-selected mrna. the cloned cdna insert of 1 kb was sequenced and a polypeptide of 241 amino acids with a molecular weight of 27 166 was deduced from the sequence. the protein was relatively rich in polar amino acids and devoid of both cysteine and tryptophan. a s ... | 1985 | 3839520 |
| correct sequence for the major nucleocapsid protein mrna of respiratory syncytial virus. | a nucleotide sequence for the mrna of the major nucleocapsid (n) protein gene of respiratory syncytial virus was reported previously (n. elango and s. venkatesen, 1983, nucleic acids res. 11, 5941-5951). however, we have been unable to confirm part of this sequence as n mrna-specific and suggest that the published sequence represents that of an aberrant chimeric transcript. here we present an alternative sequence for the n mrna and provide data supporting its authenticity. the corrected n mrna s ... | 1985 | 3839952 |
| nucleotide sequence of the g protein gene of human respiratory syncytial virus reveals an unusual type of viral membrane protein. | the major surface glycoprotein (g) of human respiratory syncytial (rs) virus has an estimated mature mr of 84,000-90,000. among a library of cdna clones prepared from rs virus mrnas, we identified clones that hybridized to a message that encoded a mr 36,000 polypeptide that was specifically immunoprecipitated with anti-g antiserum. the amino acid sequence of the g protein backbone was determined by nucleotide sequence analysis of several of the cdna clones. it contains a combination of structura ... | 1985 | 3858865 |
| respiratory syncytial virus and heart block--cause and effect? | a child aged 3 years who developed a respiratory syncytial virus infection is described. his admission was complicated by the development of an irregular pulse. he initially had a variable first or second degree heart block with transient electrocardiographic evidence suggestive of pericarditis and/or myocarditis and later developed complete heart block. he has remained asymptomatic for the past four years. a search for other causes of the heart block proved negative. it is tempting to suggest a ... | 1985 | 3859266 |
| fatal respiratory syncytial virus infection in severe combined immunodeficiency syndrome. | we encountered two cases of fatal respiratory syncytial virus infection in infants. both patients had severe anatomic abnormalities in the thymus, probably due to severe combined immunodeficiency syndrome. | 1985 | 3877455 |
| seroprevalence of respiratory syncytial virus in free-ranging bighorn sheep. | sera from 187 (42%) of 447 free-ranging bighorn sheep (ovis canadensis) in 9 western states had antibody titers to respiratory syncytial virus (rsv). titers were from 1:5 to 1:640, as determined by the standard virus neutralization test. results of the survey indicated that rsv is widespread in free-ranging bighorn sheep populations and that rsv may be an important factor in the bighorn sheep pneumonia-complex. | 1985 | 3878355 |
| survey of desert bighorn sheep in california for exposure to selected infectious diseases. | from february 1983 to june 1985, 188 desert bighorn sheep (ovis canadensis nelsoni, = 161 and oc cremnobates, = 27) from 18 herds in 17 mountain ranges and one captive herd were caught, marked, and had blood, fecal, and nasal mucus samples collected. nasal swab specimens were cultured bacteriologically and virologically specifically for parainfluenza-3 (pi-3) virus. bacterial flora differed from herd to herd. pathogenic pneumophilic bacteria (eg, pasteurella sp) seldom were found. parainfluenza- ... | 1985 | 3878356 |
| the 1a protein gene of human respiratory syncytial virus: nucleotide sequence of the mrna and a related polycistronic transcript. | the 1a mrna is the smallest mrna of human respiratory syncytial (rs) virus and encodes a single protein of approximate molecular weight 9500 as estimated previously by gel electrophoresis. the nucleotide sequence of the 1a mrna, determined from several full-length cdna clones, is reported. the 1a mrna consists of 405 nucleotides, exclusive of poly(a), with relatively long nontranslated regions at the 5' and 3' ends (84 and 126 nucleotides, respectively). the sequences at the 5' and 3' termini of ... | 1985 | 3879976 |
| monoclonal antibodies against respiratory syncytial virus and their use for rapid detection of virus in nasopharyngeal secretions. | we developed five monoclonal antibodies against respiratory syncytial virus. three of these (23a3, 12a4, and 18b2) were used in an indirect fluorescent antibody test, and the results were compared with those of a similar indirect fluorescent test with commercial anti-respiratory syncytial virus serum. the results obtained with antibody 18b2 and commercial anti-respiratory syncytial virus serum were identical, whereas with antibodies 23a3 and 12a4 the incidence of positive identifications was aro ... | 1985 | 3882752 |
| the development of monoclonal antibodies to respiratory syncytial virus and their use in diagnosis by indirect immunofluorescence. | twelve clones of murine hybridoma cells secreting antibody specific for respiratory syncytial (rs) virus were classified into four groups on the basis of their pattern of staining of unfixed rs virus-infected hep-2 cells in an indirect immunofluorescence test. three of the groups reacted with virus antigens present on the membrane of the cells, whilst the fourth group failed to stain most live cells, suggesting specificity for an antigen expressed internally. representative monoclonals from the ... | 1985 | 3884736 |
| respiratory syncytial virus. brief review. | 1985 | 3885919 | |
| effect of in vitro inoculation of bovine respiratory syncytial virus on bovine pulmonary alveolar macrophage function. | viruses may predispose the respiratory tract to the development of secondary bacterial pneumonia by impairing functions of alveolar macrophages. the effects of bovine respiratory syncytial virus (brsv) on selected functions of bovine pulmonary alveolar macrophages (pam) were examined in vitro. alveolar macrophages were obtained from nonsedated cattle, using a polypropylene tube passed intranasally into the lung. the pam lavaged from the lung were allowed to adhere to glass coverslips or plastic ... | 1985 | 3890630 |
| management of bronchiolitis. | the incidence, etiology, epidemiology, clinical presentation, treatment, prognosis, and prevention of bronchiolitis are discussed with a critical evaluation of the available studies on various therapeutic approaches. bronchiolitis is a lower respiratory-tract viral infection that affects 6-10% of all children below two years of age. respiratory syncytial virus (rsv) is the usual pathogen. the symptoms range from mild wheezing to severe respiratory distress. an infected child usually has a fever, ... | 1985 | 3891201 |
| properties of a respiratory syncytial virus isolated from a sheep with rhinitis. | a virus isolated from a yearling cross-bred ewe was identified as respiratory syncytial virus (rsv) by indirect immunofluorescence and by virus neutralization with bovine rsv antisera. the virus caused a mild conjunctivitis in 3-month-old lambs when inoculated alone. although clinical signs of pneumonia were not observed, there was gross and microscopic evidence of pulmonary inflammation in the lungs of lambs inoculated with either the sheep rsv isolate alone or in conjunction with pasteurella h ... | 1985 | 3893243 |
| quantification of respiratory syncytial virus polypeptides in nasal secretions by monoclonal antibodies. | an indirect enzyme-linked immunosorbent assay (elisa) which uses monoclonal antibody as solid-phase immunosorbent was developed to measure specific polypeptides of respiratory syncytial virus (rsv). the assay was used to examine 43 nasopharyngeal (np) aspirates from rsv-positive infants that had been examined previously for rsv by culture, direct immunofluorescence, and polyclonal antibody elisa. frozen np aspirates were serially diluted and examined for the 66k mol. wt. fusion glycoprotein (f), ... | 1985 | 3894575 |
| practical recommendations for the detection of pediatric respiratory syncytial virus infections. | in our private clinic-hospital setting, respiratory syncytial virus (rsv) was isolated from infants more frequently and sooner from nasal washes (84%; 4.2 days) than from throat swabs (45%; 5.5 days) or nasopharyngeal swabs (39%; 5.7 days). immunofluorescence of nasal wash cells identified 72% of the infants with virus isolations from nasal washes in less than one day. we therefore recommend the combination of isolation and immunofluorescence on nasal wash specimens for optimal detection of rsv- ... | 1985 | 3897268 |
| development of cell-mediated cytotoxic activity in the respiratory tract after experimental infection with respiratory syncytial virus. | the development of natural killer cell and other antibody-independent cellular cytotoxic response to rsv were studied in splenic and pulmonary mononuclear effector cells obtained from groups of 6-week-old cotton rats after subcutaneous (sc) or intranasal (in) immunization with live virulent or ultra-violet (uv) inactivated respiratory syncytial virus (rsv). no virus-induced cytotoxic activity was observed after sc immunization with live virus or in inoculation of inactivated non-infectious virus ... | 1985 | 3899431 |
| the production of monoclonal antibodies against respiratory syncytial virus and its clinical applications. | the availability of monoclonal antibodies against various viral components, either internal nucleoproteins or membrane proteins, has greatly advanced our understanding of the biochemistry of respiratory syncytial virus (rsv). the components responsible for serum neutralization have been identified by monoclonal antibodies, thus facilitating the development of synthetic rsv vaccine. a number of clinical tests have been devised using monoclonal antibodies for either qualitative or quantitative det ... | 1985 | 3899483 |
| rapid detection of respiratory syncytial virus in nasopharyngeal secretions by enzyme-linked immunosorbent assay. | a new enzyme-linked immunosorbent assay (elisa) respiratory syncytial virus antigen detection kit (ortho diagnostics, inc., raritan, n.j.) was compared with virus culture and with the indirect fluorescent antibody test (fat) by using fresh nasal washings from children with suspected respiratory syncytial virus infection. both uncentrifuged nasal washings and pellets from centrifuged split specimens were examined by elisa. the elisa was considered positive when the optical density was greater tha ... | 1985 | 3902879 |
| ribavirin treatment of respiratory syncytial viral infection in infants with underlying cardiopulmonary disease. | aerosolized ribavirin was evaluated in the treatment of respiratory syncytial virus lower respiratory tract disease in 53 infants, 36 of whom had underlying diseases. of the total infants, 26 were studied in a double-blind, placebo-controlled manner; 14 received ribavirin and 12 received placebo, a water aerosol, for an average of five days. when the infants with bronchopulmonary dysplasia and congenital heart disease treated with ribavirin were compared with those receiving placebo, the treated ... | 1985 | 3903239 |
| immunoprophylaxis and immunotherapy of respiratory syncytial virus infection in the cotton rat. | human convalescent antiserum to respiratory syncytial virus (rsv) administered intraperitoneally to cotton rats prior to rsv challenge provided near-complete protection from pulmonary infection. antiserum given subsequent to viral challenge reduced pulmonary viral titers 100-fold or greater within 24 h. sandoglobulin, a preparation of purified human igg with high titer of anti-rsv neutralizing activity, produced the same effects as convalescent antiserum. sandoglobulin was absorbed rapidly and p ... | 1985 | 3907188 |
| calf pneumonia. | infectious calf pneumonia is a high-mortality pneumonia of housed dairy-type calves. viruses are important etiologic agents and infection with bovine respiratory syncytial virus (rsv) and parainfluenza type 3 virus (pi-3 virus) may result in extensive, and sometimes fatal, lung damage. respiratory viral infections are frequently followed by mycoplasmal and secondary bacterial invasion of the lower respiratory tract, which increases the extent and severity of lung damage. bad housing, particularl ... | 1985 | 3907774 |
| bovine respiratory syncytial virus. | the current knowledge is reviewed in regards to the importance of bovine respiratory syncytial virus in the bovine respiratory disease complex. the epidemiology, clinical disease, pathologic findings, pathogenesis, diagnosis, treatment, and prevention of this viral disease are discussed. | 1985 | 3907775 |
| respiratory syncytial virus infection in immunosuppressed animals: implications in human infection. | neonatal cotton rats were treated with cyclophosphamide parenterally for three weeks before intranasal inoculation of live respiratory syncytial virus (rsv). immunosuppressive therapy resulted in severe depletion of lymphocytes from the peripheral circulation, the spleen, and the thymus. in contrast to normal rats, immunosuppressed animals developed severe pulmonary pathology with marked infiltration of foamy macrophages. persistent degeneration and regeneration of bronchial epithelial cells wer ... | 1985 | 3908616 |
| [immunofluorescent demonstration of respiratory syncytial virus infection in calves]. | specific high-titre bovine conjugated antisera were obtained against respiratory syncytial virus. use was made of the reference strain nomi and the local isolate antonovo/448. the conjugates produced were shown to have equal qualities to those of the belgian conjugate used for comparison. the anti-antonovo/448 conjugated serum was used in the direct immunofluorescent method to demonstrate a respiratory syncytial virus antigen in organs of animals with acute respiratory diseases as well as to con ... | 1985 | 3909628 |
| [acetone-celloidin method for the cytological and immunofluorescent study of cell cultures infected with various viruses]. | a method was worked out for the extraction of a cell monolayer from test tubes with the use of 10 per cent acetone solution of celloidin. this made it possible to carry out parallel cytologic and immunofluorescent studies of cultures infected with various viruses. in contrast to the alcohol-ether celloidin used in histology the use of the acetone solution of celloidin, suggested by us, preserved to a larger extent the viruses of different structure and composition, and, therefore, was shown to b ... | 1985 | 3909629 |
| [structural aspect of the respiratory syncytial virus genome]. | 1985 | 3914773 | |
| human mononuclear phagocytic cell interaction with some paramyxoviridae. | in consideration of the peculiar relationship between paramyxoviridae and immunocompetent system, we studied interactions of measles virus, newcastle-disease virus and respiratory syncytial virus with human monocytes and macrophages differentiated "in vitro". mononuclear cells were challenged with three different paramyxovirus at different multiplicities of infection. infected cells were then checked for expression of specific viral antigens by immunofluorescent test (fa). moreover, we have eval ... | 1985 | 3916331 |
| respiratory syncytial virus polypeptides. iv. the oligosaccharides of the glycoproteins. | the cell-associated glycoproteins of respiratory syncytial (rs) virus included gp1 (90k), vp70 (70k), vgp48 (48k) and gp26 (26k). although present in infected cells, there was no vp70 in purified virus. trypsin treatment of infected cells removed 80 to 90% of vp70 as well as its products vgp48 and gp26. this suggested that most of the vp70 in the cell is located on the plasma membrane. the glycoproteins of purified rs virus (gp1, vgp48 and gp26) contain mannose, galactose and fucose as well as g ... | 1985 | 3919149 |
| correlation of a disease scoring system with arterial po2 values in respiratory syncytial virus infection in calves. | the mean arterial po2 value measured in blood obtained by puncture of the brachial artery of 20 calves with acute clinical signs of a bovine respiratory syncytial virus (brsv) infection was 8.4 +/- 1.9 kpa. the values differed significantly from arterial po2 values of eleven healthy calves (mean 14.2 +/- 1.5 kpa). a disease scoring system is presented based on the type of respiration and the findings on auscultation. a high correlation (r = -0.87) was found between disease scores and arterial po ... | 1985 | 3925615 |
| respiratory syncytial virus outbreak at an institution for the mentally retarded--washington. | 1985 | 3932832 | |
| seroepidemiologic survey for antibodies to selected viruses in the respiratory tract of lambs. | a serologic study was conducted to determine the prevalence of antibodies to, and infection rate of, mastadenovirus ovi 5, m ovi 6, parainfluenza-3 (pi-3) virus, bovine herpesvirus-1 (bhv-1), respiratory syncytial virus (rsv), bovine viral diarrhea (bvd) virus, and ovine progressive pneumonia (opp) virus in lambs at a ram lamb growth-rate test station. for 2 consecutive years, serum samples were prepared from blood collected from 1- to 2-month-old ram lambs as they entered the test station (1st ... | 1985 | 3002217 |
| the effects of acute respiratory virus infection upon tracheal mucous transport. | tracheal mucous velocity was measured in 13 healthy non-smokers using a radioisotope-labeled aerosol and a multidetector probe during respiratory virus infections. the movement of boluses of tracheal mucous were either absent or reduced in number in five subjects with myxovirus infection (four influenza and one respiratory syncytial virus) within 48 hr of the onset of symptoms and in four subjects 1 wk later. one subject with influenza still had reduced bolus formation 12-16 wk after infection. ... | 1985 | 3002287 |
| bovine respiratory syncytial virus infection in an ontario cattle herd. | bovine respiratory syncytial virus was recovered from the lung of a six month old calf that died during an outbreak of respiratory disease in a cattle herd in ontario. lung tissue removed from the calf at necropsy, performed within two hours of death, was frozen at -70 degrees c prior to virus isolation attempts. syncytia and intracytoplasmic inclusions were demonstrated both in histological sections of the calf's lung and in stained cell culture preparations infected with the bovine respiratory ... | 1985 | 17422509 |
| serological evidence of respiratory syncytial virus infection in lambs. | 1985 | 16031205 | |
| respiratory syncytial virus infection in mice. | the a2 strain of human respiratory syncytial virus replicated in the nose and lung of balb/c mice, with virus growing to higher titers in older animals than in younger animals. virus was recovered from the nose between days 2 and 7 with peak titers on days 3 and 4, and from the lungs between days 2 and 9, with peak titers on days 4 through 6. serum antibody developed 2 weeks after infection. viral antigen was demonstrated in the alveolar cells of the lung by immunofluorescence. histopathological ... | 1984 | 6693171 |
| cell-mediated immunity in respiratory syncytial virus disease. | systemic cell-mediated responses to respiratory syncytial (rs) virus were detected, using a whole blood transformation assay, in 10 of 28 infants and children with rs virus infections during the period 1-14 days postadmission. cell-mediated responses were unrelated to the age of the patient or the severity of illness. no correlation was found between cellular responses and fourfold or greater rises in antibody titre to rs virus, as determined by a membrane immunofluorescence technique. patients ... | 1984 | 6693860 |
| ribavirin. | despite the plethora of antibiotics available for the treatment of bacterial infections, very few agents have been developed to treat viral diseases. ribavirin (virazole) is a triazole nucleoside antiviral agent that produces selective antiviral effects against a broad spectrum of rna and dna viruses. the drug has been effective in the treatment of naturally occurring influenza a and b infections when administered by aerosol; oral administration has been ineffective. ribavirin aerosol therapy al ... | 1984 | 6697874 |
| nucleotide sequence of the gene encoding respiratory syncytial virus matrix protein. | the amino acid sequence of the matrix protein of the human respiratory syncytial virus (rs virus) was deduced from the sequence of a cdna insert in a recombinant plasmid harboring an almost full-length copy of this gene. it specifically hybridized to a single 1,050-base mrna from infected cells. the recombinant containing 944 base pairs of rs viral matrix protein gene sequence lacked five nucleotides corresponding to the 5' end of the mrna. the nucleotide sequence of the 5' end of the mrna was d ... | 1984 | 6699948 |
| pathogens associated with acute lower respiratory tract infection in young children. | to determine the agents associated with acute lower respiratory infection in young children, we studied 102 hospitalized children less than 5 years old using culture and serology for viruses and chlamydia trachomatis, fluorescent antibody testing for pertussis and respiratory syncytial virus, blood cultures and counterimmunoelectrophoresis of nasopharyngeal secretions and urine for pneumococcal and haemophilus influenzae type b antigens. at least one agent was detected in 87 children and multipl ... | 1984 | 6701101 |
| bovine respiratory syncytial virus infections in young dairy cattle: clinical and haematological findings. | clinical signs and haematological findings during the acute and convalescent phases of an infection with bovine respiratory syncytial virus (rsv) were studied under field conditions. the study was carried out in 139 cattle less than 16 months of age in 16 herds with serologically proven bovine rsv infections. blood was collected for serological and haematological examination. repeated clinical examinations were carried out until 35 days after appearance of disease. signs of general disease such ... | 1984 | 6702077 |
| respiratory syncytial virus-related apnea in infants. demographics and outcome. | medical records of 261 hospitalized patients less than 1 year old with documented respiratory syncytial virus (rsv) infection were reviewed to determine the incidence of rsv-associated apnea and the accompanying risk of subsequent apnea or death. apnea in association with rsv infection occurred in 18% of the infants. premature birth and a young postnatal age were risk factors for development of apnea with rsv disease. apnea of prematurity appeared to be a significant risk factor for rsv apnea de ... | 1984 | 6702769 |
| [exanthematous viral diseases and measles: serology]. | a study of antibodies against measles virus and other viral agents is made to investigate possible existence of a false measles or "measles-like" in 48 children with this disease during an epidemic. hemagglutination-inhibiting technique was used to determine rubella antibodies, and complement fixation technique to measure those produced against other diseases studied. results were grouped according to the seroconversions found. the following serologic diagnoses were found: twenty eight cases of ... | 1984 | 6703539 |
| purification and characterization of gp90, one of the envelope glycoproteins of respiratory syncytial virus. | the large glycoprotein, gp90, of respiratory syncytial virus (rsv) was purified by affinity chromatography using a monoclonal antibody. hyperimmune rabbit antiserum directed specifically to the purified gp90 neutralized rsv to high titre but did not inhibit fusion of previously infected cells. 125i-labelled gp90 bound rapidly to hep-2 cells in an unsaturable manner; binding was inhibited by the hyperimmune rabbit antiserum. | 1984 | 6707612 |
| [isolation of the respiratory syncytial virus from calves with respiratory diseases]. | described is a respiratory enzootic in calves aged 30 to 90 days. two cytopathic strains of the bovine respiratory syncytial virus were isolated from diseased calves in a cell culture of lamb thyroid. clinical observations and the results of virologic and serologic investigations have revealed that mixed infections of the respiratory syncytial virus and the mucosa disease virus take part in the etiology of the respiratory enzootic described. | 1984 | 6730327 |
| an outbreak of respiratory syncytial virus infection in an old people's home. | at least 20 of the 50 residents of an old people's home suffered from an acute respiratory infection and four of them died. respiratory syncytial virus was isolated from two of the patients. the importance of this virus in the elderly is probably underestimated. | 1984 | 6736667 |
| some respiratory and enteric diseases of cattle: an update. | fibrinous pneumonia caused 40-45% of death losses in cattle shipped into bruce county, ontario. feeding corn silage within the first 2 weeks of arrival increased mortality. commingling cattle from different sources and keeping more than 110 cattle in a pen were detrimental. while use of antimicrobials in the starter ration was beneficial, prophylactic use of antimicrobials in the water was not. mortality was directly proportional to morbidity and treatment costs. vaccinating against any respirat ... | 1984 | 6738491 |
| infection rates of respiratory syncytial virus in pediatric patients attending phra mongkutklao hospital, bangkok. | respiratory syncytial virus (rsv) and other pathogens were isolated from nasopharyngeal secretions from 200 pediatric patients attending the out patient department of phra mongkutklao hospital with symptoms of upper respiratory tract infections. their sera were also taken for determination of class specific immunoglobulin antibody titers. the positive isolation rates were 36% for rsv, 5.5% for adenovirus 1.5% for herpes simplex virus (hsv), and 4% for staphylococcus aureus. one to 5.5% of these ... | 1984 | 6740381 |
| virus-induced complement activation and neutrophil-mediated cytotoxicity against respiratory syncytial virus (rsv). | complement-dependent neutrophil-mediated cytotoxicity (cdnc) was determined by specific release of 51-chromium (51cr) from respiratory syncytial virus infected hep2 cells in a microcytotoxicity assay. there was significant release of 51cr from rsv infected cells as compared to uninfected cells in the presence of complement (c) and neutrophils (pmn). the degree of cytotoxicity was dependent upon the concentration of c used in the assay. such cytotoxicity was effectively abolished after heat-inact ... | 1984 | 6744659 |
| comparison of immediate and delayed inoculation of hep-2 cells for isolation of respiratory syncytial virus. | immediate inoculation of hep-2 cells is generally advocated for the isolation of respiratory syncytial virus. however, delayed laboratory inoculation of properly transported specimens obtained by aspiration of nasopharyngeal mucus provided an isolation rate similar to that obtained with immediate inoculation. | 1984 | 6746883 |
| diagnosis of respiratory syncytial virus infection in children: comparison of viral antigen detection and serology. | direct detection of viral antigen in nasopharyngeal secretion by radioimmunoassay was compared with serology by igg antibody enzyme immunoassay for diagnostic efficacy in 77 children with clinically suspected respiratory syncytial virus (rsv) infections. antigen detection gave a positive diagnosis in 26 of 33 (79%) children in whom rsv infection was diagnosed by any of the two methods. the diagnostic efficacy of antigen detection was dependent upon the interval after onset at which specimens wer ... | 1984 | 6747612 |
| immunoglobulin class-specific antibody response in respiratory syncytial virus infection measured by enzyme immunoassay. | immunoglobulin class-specific enzyme immunoassay (eia) was used for determination of antibody responses in sera collected from 26 children with acute primary respiratory syncytial virus (rsv) infections. all 26 patients had igg antibody responses with a significant titer increase in 24 (92%); an igm antibody response was detected in 19 of the 26 (73%). from patients aged 6 months or less only 5 of 8 produced detectable igm antibodies, whereas all patients aged 1-2 years did so. igm antibodies ap ... | 1984 | 6747613 |
| bronchopulmonary obstruction in children with respiratory virus infections. | among 873 children hospitalized with confirmed respiratory viral infections, 492 suffered from bronchopulmonary obstruction. respiratory syncytial virus infections were dominant among the patients with bronchopulmonary obstruction (80%). the majority of patients with rhinovirus infections suffered from bronchopulmonary obstruction (57%). two main groups of children at risk from developing bronchopulmonary obstruction during respiratory virus infections were found: 1) infants, suffering from bron ... | 1984 | 6321218 |
| treatment of respiratory viral infection in an immunodeficient infant with ribavirin aerosol. | an infant with severe combined immunodeficiency syndrome (scids) secondary to adenosine deaminase deficiency had pneumonitis and combined infection with respiratory syncytial virus (rsv) and parainfluenza virus type 3 (piv3). four separate courses of ribavirin were delivered by small-particle aerosol. the piv3 disappeared during the first course, and rsv disappeared after the fourth course on the 58th hospital day. neither virus returned during profound immunosuppression for bone marrow transpla ... | 1984 | 6322573 |
| a prospective study of chlamydial, mycoplasmal, and viral infections in a neonatal intensive care unit. | in a prospective study of non-bacterial infection in a neonatal intensive care unit in north west london, chlamydia trachomatis infection was identified in 4 of 280 babies (1.4%) and was the most common cause of neonatal ophthalmia. one of the four developed pneumonitis. ureaplasma urealyticum was found to colonise the nasopharynx in 53 of 235 babies (22.6%), with mycoplasma hominis present in 6 of 235 babies (2.6%). there was a statistically significant association between u urealyticum colonis ... | 1984 | 6322705 |
| an enzyme-linked immunosorbent assay (elisa) for igg and iga antibodies to respiratory syncytial virus in low dilutions of secretions of human serum and secretions. | an enzyme-linked immunosorbent assay (elisa) has been developed for titration of igg and iga antibodies to respiratory syncytial (rs) virus in low dilutions of human serum, colostrum, and nasopharyngeal secretions. previously the sensitivity of rs virus elisa on such specimens has been limited by nonspecific absorption of antibody, particularly iga, to crude antigen preparations. for igg antibody estimation in infant sera, this unwanted binding was reduced to workable levels by increasing the se ... | 1984 | 6389773 |
| antibiotic treatment of pneumonia and bronchiolitis. a prospective randomised study. | routine administration of antibiotics in the treatment of pneumonia and bronchiolitis in infants and small children was evaluated in an open randomised prospective trial. from 1979-82 136 children between the age of 1 month and 6 years were allocated to one of two treatment groups shortly after their admission to a paediatric ward. group a patients were to be given antibiotics but those in group b were not. none of the children had received antibiotics before hospital admission. a viral infectio ... | 1984 | 6391389 |
| suppression of respiratory syncytial virus infection in cotton rats by bis(5-amidino-2-benzimidazolyl)methane. | intraperitoneal administration of bis(5-amidino-2-benzimidazolyl)methane at well-tolerated daily doses of 25 mg/kg subsequent to challenge and for 3 days thereafter effected over a 1-log reduction in the amount of virus recovered from lungs of cotton rats inoculated intratracheally with respiratory syncytial virus. when animals were immunosuppressed to prolong virus shedding, the reduction in recovered virus achieved with a 7-day dosing schedule of bis(5-amidino-2-benzimidazolyl)methane exceeded ... | 1984 | 6393863 |
| [rapid diagnosis of respiratory syncytial virus in children with bronchiolitis by indirect immunofluorescence technic]. | 1984 | 6400166 | |
| respiratory syncytial virus infection in the elderly. | 1984 | 6419871 | |
| respiratory viruses and sudden infant death. | viruses were shown to be present in the respiratory tract in 200 of 763 cases of the sudden infant death syndrome studied in the nine years 1974-82. epidemiological and pathological evidence suggested that the distribution of viruses in the sudden infant death syndrome differs between infants aged 3 months or less and those aged over 3 months: the incidence of detection of virus was 14% in the younger group compared with 39% in the older group. the distribution of the viruses in these two groups ... | 1984 | 6426615 |
| management of respiratory failure in infants with acute viral bronchiolitis. | fifteen infants with acute viral bronchiolitis required mechanical ventilation. infants were all aged less than 12 weeks and all had evidence of atelectasis or pneumonia on chest x-ray films. respiratory syncytial virus was identified by immunofluorescence in 14 of 15 patients. intubation and mechanical ventilation were initiated to reduce the work of breathing rather than to treat hypoxemia alone. additional therapy included continuous positive airway pressure or positive end-expiratory pressur ... | 1984 | 6437212 |
| the characterization and uses of monoclonal antibodies to respiratory syncytial virus. | fifteen hybridomas secreting monoclonal antibodies against respiratory syncytial virus (rsv) have been produced. three react with nucleoprotein (mw 42 000 daltons), 1 with phosphoprotein (mw 36 000 daltons), 1 with a larger protein (mw 60 000 daltons) and 10 with the fusion glycoprotein (mw 46 000 + 22 000 daltons). by immunofluorescent staining of infected cells, 5 monoclonal antibodies bind to cytoplasmic inclusions and the remainder give diffuse cytoplasmic fluorescence. virus infectivity is ... | 1984 | 6084614 |
| detection of respiratory virus antigens in nasopharyngeal secretions from patients with acute respiratory disease by radio-immunoassay and tissue culture isolation. | an investigation was made of the sensitivity and specificity of four-layer radio-immunoassays (ria) in the detection of adenovirus, respiratory syncytial virus, influenza virus types a and b, as well as para-influenza virus types 1, 2 and 3 from nasopharyngeal aspirates of 146 patients with acute respiratory disease. the sensitivity of ria was comparable with that of tissue culture isolation if the total number of positives is considered. the difference may have been caused both by a higher effi ... | 1984 | 6088961 |
| prevalence of antibodies to bovine respiratory syncytial virus, bovine viral diarrhea virus, bovine herpesvirus-1, and bovine parainfluenza-3 virus in sheep and goats in quebec. | serum samples were collected from 1,075 clinically normal sheep and goats from 77 flocks in 7 agricultural regions of quebec from june to august 1982. sheep and goats were tested for antibodies to bovine respiratory syncytial virus, bovine viral diarrhea virus, and bovine herpes-virus-1 by the indirect fluorescent antibody technique and for parainfluenza-3 virus by the hemagglutination inhibition test. the prevalence of antibodies in animals to respiratory syncytial virus was 31%; to bovine vira ... | 1984 | 6089626 |
| survey for antibodies to respiratory viruses in two groups of sheep in northern ireland. | two hundred serum samples from texel and texel crossbred sheep (non-indigenous breeds) and 200 from indigenous northern ireland breeds (mainly blackface, cheviot and border leicester crosses) were tested for antibodies to parainfluenza virus types 1, 2 and 3, respiratory syncytial virus, bovine adenovirus (subgroups 1 and 2), influenza type a, maedi-visna virus and bovine virus diarrhoea virus. the percentage of animals with antibodies to parainfluenza virus 3 (50 to 56 per cent) and adenovirus ... | 1984 | 6095511 |