Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| complement activation by respiratory syncytial virus-infected cells. | respiratory syncytial virus (rsv), a major respiratory pathogen of children, has been speculated to cause disease by immunologic mechanisms. although circulating levels of complement (c) are normal during rsv infections, the role of c in respiratory tract secretion is unclear. since epithelial cells of the respiratory tract of children infected with rsv express viral surface antigens, the ability of rsv infected human cells to activate c was studied. rsv infected human cells (hela) were found to ... | 1986 | 3954599 |
| vapors, viruses, and views. ribavirin and respiratory syncytial virus. | 1986 | 3953524 | |
| seroepizootiologic study of bovine respiratory syncytial virus in a dairy herd. | a 16-month seroepizootiologic study of bovine respiratory syncytial virus (brsv) infection was conducted in a dairy herd. results indicated that antibodies to brsv present in serum from newborn calves were derived through the ingestion of colostrum. this passive immunity in calves became undetectable in an average of 99 days (sd = 36.5; range = 30 to 208 days). two epizootics of respiratory tract disease occurred during the study period, and an association with brsv was demonstrated in both epiz ... | 1986 | 3954198 |
| age related igg subclass response to respiratory syncytial virus fusion protein in infected infants. | respiratory syncytial virus (rsv) fusion protein was purified by immunoaffinity chromatography using a mouse monoclonal antibody coupled to affi-gel 10. the fusion protein was homogeneous by sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) and free of other detectable viral or cellular protein. the purified fusion protein was used in a quantitative enzyme-linked immunosorbent assay (elisa) to measure the age-related antibody response to this protein in infected infants. the f ... | 1986 | 3791688 |
| serum and nasal-wash immunoglobulin g and a antibody response of infants and children to respiratory syncytial virus f and g glycoproteins following primary infection. | an enzyme-linked immunosorbent assay (elisa) with immunoaffinity-purified fusion (f) or attachment (g) glycoprotein was used to measure the serum and secretory immune responses of 18 infants and children, 4 to 21 months of age, who underwent primary infection with respiratory syncytial virus (rsv). most of the 10 older individuals (9 to 21 months of age) developed moderate levels of serum and nasal-wash immunoglobin a (iga) and igg f and g antibodies. these individuals developed a moderate level ... | 1986 | 3754878 |
| study on the etiologic role of bovine respiratory syncytial virus in pneumonia of dairy calves. | bovine respiratory syncytial virus (brsv) was the viral agent most commonly identified in 14 epizootics of pneumonia in dairy calves. a microtiter serum-virus neutralization test proved to be the best means of identifying involvement of brsv; seroconversion (fourfold or greater rise in titer) was demonstrated in 10 of the 14 epizootics. only limited involvement of bovine viral diarrhea virus, infectious bovine rhinotracheitis virus, parainfluenza 3 virus, and bovine adenovirus type 3 was recogni ... | 1986 | 3733504 |
| a neutralizing monoclonal antibody to respiratory syncytial virus which binds to both f1 and f2 components of the fusion protein. | a virus-neutralizing monoclonal antibody (1e3) specifically immunoprecipitated the 70000 mol. wt. (70k) fusion (f) protein from respiratory syncytial (rs) virus-infected hela cells. western blotting analysis of polypeptides from such cells separated by sds-polyacrylamide gel electrophoresis (sds-page) under reducing conditions revealed that 1e3 was peculiar in that it bound to both f1 (50k) and f2 (20k) components of the f protein. antibody subsequently eluted from either the f1 or the f2 region ... | 1986 | 3755162 |
| serologic evidence of respiratory syncytial virus infection in free-ranging mountain goats (oreamnos americanus). | 1986 | 3735589 | |
| isolation and serologic evidence of a respiratory syncytial virus in bighorn sheep from colorado. | 1986 | 3735590 | |
| respiratory syncytial virus and ribavirin. | 1986 | 3763427 | |
| [significance of various respiratory viruses in the development of chronic nonspecific bronchopulmonary processes]. | combined virological and bacteriological studies were carried out in 83 patients with chronic bronchopulmonary diseases. viral antigens, predominantly respiratory syncytial virus antigen, were detected in 72% of the patients in bronchial material obtained by bronchoscopy. examinations for antibody of igm and igg classes specific for different viruses revealed acute infection in 79% of the patients which coincided with exacerbation of the process due to concomitant arvd. the other patients had ch ... | 1986 | 3765557 |
| bovine respiratory syncytial virus vaccine. | 1986 | 3771353 | |
| effect of age and preexisting antibody on serum antibody response of infants and children to the f and g glycoproteins during respiratory syncytial virus infection. | the serum antibody response of 50 infants and children infected with respiratory syncytial virus (rsv) was determined by a glycoprotein-specific enzyme-linked immunosorbent assay, and the effects of age and preexisting antibody titer at the time of rsv infection on response to the g and f glycoproteins of rsv were examined. the immune response to the g and f glycoproteins was assessed with anti-human immunoglobulin a to permit measurement of the response of young infants in the presence of mater ... | 1986 | 3771779 |
| dissociation between serum neutralizing and glycoprotein antibody responses of infants and children who received inactivated respiratory syncytial virus vaccine. | the serum antibody response of infants and children immunized with formalin-inactivated respiratory syncytial virus (rsv) vaccine 20 years ago was determined by using an enzyme-linked immunosorbent assay specific for the rsv fusion (f) and large (g) glycoproteins and a neutralization assay. twenty-one young infants (2 to 6 months of age) developed a high titer of antibodies to the f glycoprotein but had a poor response to the g glycoprotein. fifteen older individuals (7 to 40 months of age) deve ... | 1986 | 3755730 |
| serum immunoglobulin g antibody subclass responses to respiratory syncytial virus f and g glycoproteins after primary infection. | because the immunoglobulin g (igg) response to carbohydrate antigens is typically from the igg2 subclass and the igg response to protein antigens is typically from the igg1 and sometimes the igg3 subclass, two respiratory syncytial virus glycoproteins, f and g, which differ substantially in the amount of glycosylation, were used as antigens in an enzyme-linked immunosorbent assay to determine igg subclass responses in 20 infants and young children with naturally acquired respiratory syncytial vi ... | 1986 | 3755731 |
| [reproductive activity of the influenza a virus in the splenocytes of experimental animals with mono- and mixed infections]. | virus-induced processes in organs and tissues of syrian hamsters in relation to the influenza a virus strain used (hon1 or h3n2), age of the animals, and in the presence of mixed infection were compared. the infection of young hamsters with a/pr8/34 and a/bangkok/1/79 viruses was shown to induce the synthesis of viral proteins np and m in spleen cells lasting for up to 15 days (the observation period). in mixed influenza and respiratory syncytial virus infection the possibility of influenza viru ... | 1986 | 3765566 |
| predictive value of respiratory syncytial virus-specific ige responses for recurrent wheezing following bronchiolitis. | to determine whether the magnitude of the respiratory syncytial virus (rsv)-specific ige response at the time of an episode of rsv bronchiolitis in infancy accurately predicts the development of subsequent wheezing episodes, we observed 38 infants prospectively from the time of an episode of infantile bronchiolitis through 48 months of age. peak rsv-ige titers were measured at the time of the bronchiolitis episode using an elisa procedure. notation was made of both the number of subsequent wheez ... | 1986 | 3772657 |
| human respiratory syncytial virus glycoprotein g expressed from a recombinant vaccinia virus vector protects mice against live-virus challenge. | recombinant vaccinia virus vectors were constructed which expressed the major surface glycoprotein g of human respiratory syncytial (rs) virus. the biological activity of the g protein expressed from these vectors was assayed. inoculation of rabbits with live recombinant virus induced high titers of antibody which specifically immunoprecipitated rs virus g protein and was capable of neutralizing rs virus infectivity. immunization of mice by either the intranasal or the intraperitoneal route with ... | 1986 | 3773052 |
| respiratory syncytial virus: a report of a 5-year study at a children's hospital. | a study over a 5-year period (1979-1983) of rsv infections in children in sydney, australia is reported. in common with findings made elsewhere in the world, annual epidemics of rsv infection commencing in autumn and lasting 4-6 months, with peak activity in mid-winter, were observed in 1979, 1980, and 1983. however, in 1981 and 1982 virus activity was first detected in midsummer, peaked in autumn, and was present throughout most months of the year. the alteration in virus activity in 1981 and 1 ... | 1986 | 3746259 |
| structural differences between subtype a and b strains of respiratory syncytial virus. | differences in the properties of homologous intracellular structural components of eight strains of subtype a and eight strains of subtype b of human respiratory syncytial (rs) virus were examined. the size of the fusion (f) protein cleavage products and the phosphoprotein (p) showed systematic differences between virus strains representing the two subtypes. the apparent mol. wt. in sds-polyacrylamide gels under reducing conditions was 48,000 (48k) and 46k to 47k for the cleavage product f1 in s ... | 1986 | 3540207 |
| comparison of washed nasopharyngeal cells and whole nasal secretions for detection of respiratory syncytial virus antigens by enzyme-linked immunosorbent assay. | we compared washed nasal epithelial cells with unfractionated nasal secretions as sources of respiratory syncytial virus (rsv) antigens in an indirect enzyme-linked immunosorbent assay (elisa). of 28 infants positive for rsv by virus isolation or direct immunofluorescence or both, 27 (96%) were positive by elisa with whole nasal secretions, whereas only 19 (68%) were positive by elisa with the matching washed-cell fractions. furthermore, the elisa absorbances obtained with nasal secretions were ... | 1986 | 3700621 |
| enhanced isolation of respiratory syncytial virus in cell culture. | during two winter seasons, we found that the combination of wi-38 or mrc-5 human lung fibroblasts plus primary rhesus monkey kidney (rhmk) and hep-2 cell cultures yielded maximal isolation of respiratory syncytial virus. cytopathic effects (cpe) developed earliest in rhmk cells and slowest in the human fibroblast lines. in rhmk cells, 50% of ultimately positive cultures showed cpe in 5 days, and 90% of positive cultures showed cpe within 7 days during both respiratory syncytial virus seasons. | 1986 | 3700634 |
| measles virus rna detected in paget's disease bone tissue by in situ hybridization. | morphological and immunocytological studies have demonstrated the presence of paramyxovirus antigens in paget's bone disease tissue and in particular antigens related to measles virus and respiratory syncytial virus. to examine the relationship between measles virus and paget's bone disease we used in situ hybridization and a cloned measles virus dna probe specific for the nucleocapsid protein to detect and locate measles virus rna sequences in paget's bone tissue. in five patients with the dise ... | 1986 | 3701300 |
| risk of primary infection and reinfection with respiratory syncytial virus. | respiratory syncytial virus is the most important cause of serious lower respiratory tract infection in children. for children followed up from birth in the houston family study, the infection rate was 68.8/100 children less than 12 months of age and 82.6/100 during the second year of life. virtually all children had been infected at least once by 24 months of age, and about one half had experienced two infections. although lower respiratory tract disease (lrd) was common (22.4/100 during year 1 ... | 1986 | 3706232 |
| murine pneumonia virus: seroepidemiological evidence of widespread human infection. | more than 75% of a random sample of adult human sera exhibited moderate to high murine pneumonia virus (pvm)-neutralizing activity. there was no correlation between pvm-neutralizing activity and respiratory syncytial virus or parainfluenza type 3 virus-neutralizing activities of the same sera. in children the proportion of sera with moderate to high titres increased with age, indicating early exposure to infection. seroconversion (i.e. greater than fourfold increase in titre) was observed in fou ... | 1986 | 3711866 |
| simplified enzyme-linked immunosorbent assay for specific antibodies to respiratory syncytial virus. | a simplified and reliable enzyme-linked immunosorbent assay (elisa) was applied to the detection of serum antibodies against respiratory syncytial virus (rsv). rsv-infected cells were fixed and dried on 96-well microtiter plates and kept at 4 degrees c. the titers of reference sera were determined by endpoint dilution. a linear relation was found between the titers and the logarithm of absorbance values of sera diluted to 1:1,000 (r = 0.93, p less than 0.001). measurement of rsv antibodies was d ... | 1986 | 3771748 |
| the use of purified immunoglobulin in the therapy of respiratory syncytial virus infection. | 1986 | 3714524 | |
| [respiratory syncytial virus infections in newborn infants]. | twenty-three of 23 neonates were contaminated in the course of an outbreak of respiratory syncytial virus (rsv) in a neonatal care unit. symptoms among 22 infected symptomatic infants included rhinitis (n = 21), dyspnea (n = 19), cough (n = 17), apnea (n = 5), seizures (n = 3), fever (n = 3). five patients presented with severe respiratory distress. the occurrence of non-obstructive apnea was significantly correlated with a history of respiratory disease, rsv infection during the first 15 days o ... | 1986 | 3718165 |
| culture of respiratory syncytial virus infected diploid bovine nasal mucosa cells on cytodex 3 microcarriers. | in preliminary studies, a system for the propagation of respiratory syncytial virus (rsv) using cytodex 3 microcarriers was established. optimal growth conditions were defined in culture to be a microcarrier concentration of 1.5 g/l with a cell inoculum density of 4 x 10(4) cells/ml tissue culture medium. under these conditions growth coefficients were 3-fold greater in microcarriers when compared to conventional monolayer culture in roux culture flasks. maximum yield of virus antigen was achiev ... | 1986 | 3718237 |
| a sero-epidemiological survey of infections with the bovine respiratory syncytial virus in first-season grazing calves. | 1986 | 3751394 | |
| neutralizing activity in human milk fractions against respiratory syncytial virus. | neutralizing activity against respiratory syncytial virus (rsv) was measured in milk samples from 17 healthy women whose infants had an acute infection with respiratory syncytial virus (rsv) and from 27 women with healthy infants. all milk samples were obtained 2-8 months post partum. neutralizing activity was detected in 36 samples. no major difference in neutralizing titers was observed between the two groups, and the titers were low. rsv-specific iga was found in two samples, and rsv-specific ... | 1986 | 3564936 |
| recent advances in antiviral therapy. | virus replication is described, and the clinical trials and indications for amantadine, rimantadine, vidarabine, vidarabine phosphate, acyclovir, ribavirin, and other promising antiviral agents are reviewed. amantadine and rimantadine are useful for the treatment and prophylaxis of viral influenza a infections. vidarabine is a second-line agent and is effective for the treatment of herpes simplex encephalitis, neonatal herpes simplex types 1 and 2, and varicella-zoster infections. vidarabine pho ... | 1986 | 3542344 |
| time-resolved fluoroimmunoassay with monoclonal antibodies for rapid diagnosis of influenza infections. | monoclonal antibodies that are broadly reactive with either influenza a or influenza b viruses were used to develop a 2- to 3-h antigen capture time-resolved fluoroimmunoassay (tr fia) for detecting influenza viral antigens in both original nasopharyngeal aspirate specimens and in tissue cultures inoculated with nose or throat swab specimens. the lower limit of sensitivity of the assay was about 10 pg of protein as determined with purified influenza a nucleoprotein expressed by recombinant dna. ... | 1986 | 3537001 |
| [enhanced luminescence intensity in immunofluorescent preparations following trypsin treatment]. | comparative investigations were carried out on the immunofluorescent preparations of cell cultures infected with bovine viruses--rota-, corona-, respiratory-syncytial, parainfluenza-3, adeno-1, and herpes-1--to test various fixatives and the effect of trypsin in raising the sensitivity of the immunofluorescence method. the effect of trypsin was manifested in fixation with formalin, ethanol, methanol, and acetone treated immunofluorescent preparations of cell cultures infected with rota- and aden ... | 1986 | 3544471 |
| rapid diagnosis of respiratory syncytial virus infection by antigen immunofluorescence detection with monoclonal antibodies and immunoglobulin m immunofluorescence test. | during a respiratory syncytial virus (rsv) infection outbreak in a pediatric hospital, diagnosis was made by immunofluorescence on smears by using an anti-rsv monoclonal antibody (ifm). immunoglobulins m and g were titrated by indirect immunofluorescence on hep-2 cells infected with an rsv strain. the ifm was sensitive (89%) and specific (75%) when compared with the cell culture method. we showed that the specimens which were found positive by ifm and negative by cell culture were truly positive ... | 1986 | 3536995 |
| capture-elisa for serum igm antibody to respiratory syncytial virus. | a four-component solid-phase capture enzyme immunoassay was set up to test for serum igm antibody to respiratory syncytial (rs) virus and was compared with immunofluorescence assay (ifa). a total of 128 young children with acute respiratory infections were studied. thirty-six were shown to be rs virus-positive by the detection of rs virus in nasopharyngeal secretions and 92 were rs virus-negative. a serum specimen was collected after admission to the hospital (days 0-4) and a further specimen wa ... | 1986 | 3540115 |
| presence of respiratory viruses in middle ear fluids and nasal wash specimens from children with acute otitis media. | during a 28-month period, 84 children with acute otitis media were studied by viral and bacterial cultures of middle ear fluid and viral cultures of nasal lavage fluid. viruses were isolated from the middle ear fluid of 17 (20%) patients. evidence of viral infection was demonstrated by positive viral cultures of middle ear fluid and/or nasal lavage fluid in 33 (39%) patients. rhinovirus in one patient and influenza b virus in another were the only pathogens isolated. influenza virus, enterovirus ... | 1986 | 3010225 |
| rs virus diagnosis: comparison of isolation, immunofluorescence and enzyme immunoassay. | two techniques for rapid diagnosis, immunofluorescence (ifat) and enzyme immunoassay (eia), have been compared with virus isolation in tissue culture for the detection of respiratory syncytial virus (rsv) in specimens of nasopharyngeal secretions. the specimens were obtained from children under five years of age suffering from acute respiratory illness, during a period of six months from january to june 1982. of 471 specimens examined 54 (11.5%) were positive by virus isolation and 180 (38.2%) w ... | 1986 | 3295470 |
| isolation of viruses from specimens submitted for direct immunofluorescence test for respiratory syncytial virus. | 1986 | 3511204 | |
| respiratory syncytial virus: a review of the virus, its epidemiology, immune response and laboratory diagnosis. | 1986 | 3524530 | |
| strategy for efficient detection of respiratory viruses in pediatric clinical specimens. | direct immunofluorescence (if) with a polyclonal respiratory syncytial virus (rsv)-specific antibody preparation was used for antigen detection during the 1982-1983 rsv season (155 specimens) and gave an overall sensitivity of 94% with 87% specificity compared with viral culture. indirect if was used in the 1983-1984 season (265 specimens) and exhibited sensitivity of 96% with specificity of 79%. during these two seasons, 42 of 224 (18.8%) specimens that were if-negative for rsv grew viruses oth ... | 1986 | 3536275 |
| comparison of two new tests for rapid diagnosis of respiratory syncytial virus infections by enzyme-linked immunosorbent assay and immunofluorescence techniques. | the sensitivity and the specificity of two new commercial reagent tests, an indirect fluorescent antibody test (fat) with a mouse monoclonal antibody (mab) against respiratory syncytial virus (rsv) and an enzyme-linked immunosorbent assay (elisa) rsv antigen detection kit, were determined by a comparison of results from these tests with those of tissue culture isolation and an indirect fat with bovine polyclonal antibody (bpa). of 251 nasal aspirates from infants with suspected rsv infection, po ... | 1986 | 3536993 |
| application of a modified indirect fluorescent antibody test to the detection of antibodies to bovine respiratory syncytial virus in ontario cattle. | a modified indirect fluorescent antibody test for the detection of serum antibodies to bovine respiratory syncytial virus was developed. the test made use of terasaki plastic microtiter plates in which bovine respiratory syncytial virus (saskatchewan strain) infected georgia bovine kidney cells were grown and fixed in situ by a modified acetone fixation procedure. evans blue dye was used as a counterstain to reduce nonspecific fluorescence. in a study of 986 field sera from a geographically broa ... | 1986 | 3527392 |
| expression of the f glycoprotein of respiratory syncytial virus by a recombinant vaccinia virus: comparison of the individual contributions of the f and g glycoproteins to host immunity. | a cdna clone representing the mrna coding sequence of the fusion glycoprotein (f) gene of human respiratory syncytial virus (rsv) was constructed and inserted into the thymidine kinase gene of vaccinia virus (wr strain) under the control of a vaccinia virus promoter. the resulting recombinant vaccinia virus, vaccinia f, expressed the f1 and f2 cleavage products (48 and 20 kda, respectively) of the f glycoprotein in cell culture. f1 and f2 were indistinguishable from their authentic rsv counterpa ... | 1986 | 3532115 |
| resistance to human respiratory syncytial virus (rsv) infection induced by immunization of cotton rats with a recombinant vaccinia virus expressing the rsv g glycoprotein. | a cdna copy of the g glycoprotein gene of human respiratory syncytial virus (rsv) was placed under control of a vaccinia virus promoter and inserted into the thymidine kinase locus of the vaccinia virus genome. the recombinant vaccinia virus retained infectivity and expressed a 93-kda protein that migrated with the authentic rsv g glycoprotein upon polyacrylamide gel electrophoresis. glycosylation of the expressed protein and transport to the cell surface were demonstrated in the absence of othe ... | 1986 | 3513191 |
| a live pasteurella haemolytica vaccine efficacy trial. | a live pasteurella haemolytica serotype 1 vaccine was used in an efficacy trial conducted on 100 lightweight feeder calves purchased from a florida ranch. forty-one calves were inoculated with the vaccine intradermally in the neck. fifty-nine calves served as nonvaccinated controls. fourteen days later, the calves were shipped to an order buyer in eastern tennessee, where the calves were mixed with 60 local calves in a community sale barn for 72 hours. after 3 additional days, the calves were sh ... | 1986 | 3514562 |
| rapid detection of respiratory syncytial virus in nasopharyngeal aspirates by a commercial enzyme immunoassay. | a commercial enzyme immunoassay (eia) for the rapid detection of respiratory syncytial virus (rsv) in respiratory secretions was evaluated by comparison with both virus isolation in hep-2 cells and indirect immunofluorescence (ifa) staining of exfoliated respiratory cells. initial examination of 80 nasopharyngeal aspirates collected from infants with acute respiratory illness showed that the rsv eia was positive for 21 of 24 specimens positive by virus isolation or ifa (87.5% sensitivity) and ne ... | 1986 | 3514658 |
| detection of respiratory syncytial virus in nasopharyngeal secretions by enzyme-linked immunosorbent assay, indirect immunofluorescence, and virus isolation: a comparative study. | an enzyme-linked immunosorbent assay (elisa) was developed for the detection of respiratory syncytial virus (rsv) antigens in nasopharyngeal secretions (nps) from children with acute respiratory disease. antisera against rsv nucleocapsids were used as immunoreagents for this test system. the results obtained by rsv antigen elisa were compared to those of indirect immunofluorescence (if) and tissue culture virus isolation (tc). of the 404 nps obtained, 278 were tested in parallel by elisa and if ... | 1986 | 3522805 |
| ribavirin aerosol for acute bronchiolitis. | a randomised double blind placebo controlled trial of treatment with an aerosolised antiviral agent, ribavirin, was conducted in 26 infants with clinically diagnosed bronchiolitis. nebulised ribavirin (14 infants) or normal saline aerosol (12 infants) was given for 18 hours a day for at least three days. respiratory syncytial virus was identified in nasal secretions from 20 cases (10 from both groups). trends in seven out of eight clinical variables favoured active treatment. ribavirin aerosol w ... | 1986 | 3524473 |
| use of egg-yolk antibody for detection of respiratory syncytial virus in nasal secretions by elisa. | egg-yolk immunoglobulins extracted from the eggs of hens immunized with respiratory syncytial virus (rsv) have been used as a reagent in double sandwich elisa for detecting rsv in nasal secretions. the sensitivity of virus detection was the same in indirect elisa, using rabbit anti chicken globulin conjugate, as when biotinylated yolk globulin and labeled avidin were used for detection. the specificity of elisa for detecting rsv using yolk antibody was similar to that achieved by indirect immuno ... | 1986 | 3524512 |
| flunixin meglumine in calves with natural bovine respiratory syncytial virus infection. | twenty-three calves (three to eight months of age) with serological evidence of bovine respiratory syncytial virus infection were used in this study. the calves originated from four herds with respiratory tract disease. in a double blind trial the calves were injected intravenously with either flunixin meglumine (2 mg/kg body weight) or with a placebo. the effect on the course of disease was measured using the po2 in capillary blood samples from the ears of the calves and by the effect on body t ... | 1986 | 3511602 |
| antigenic variation between human respiratory syncytial virus isolates. | three hybridoma antibodies, prepared against the rsn-2 strain of human respiratory syncytial (rs) virus, have been used to identify antigenic variation between 41 isolates of rs virus collected from widely separated geographical regions over a period of 29 years. one antibody was directed against an antigenic site on the virus fusion protein, vp70. this site was shared by 21 virus isolates tested and its recognition by the antibody was sensitive to the presence of 2-mercaptoethanol. the remainin ... | 1986 | 3517224 |
| evaluation of clinical specimens for the presence of respiratory syncytial virus antigens using an enzyme immunoassay. | an enzyme-linked immunoassay (eia) was developed for the detection of respiratory syncytial virus (rsv) antigen in nasopharyngeal secretions. this assay, which employs goat and rabbit anti-rsv as the capture and detector antibodies respectively, was used in a retrospective evaluation of frozen clinical specimens from children. the eia results were compared with those of virus isolation in cell culture and direct fluorescent antibody staining performed at the time of specimen collection. the sens ... | 1986 | 3517226 |
| value of rapid diagnosis of respiratory syncytial virus infection on management of small infants. | respiratory syncytial virus (rsv) is a common cause of infection in infancy and early childhood. a presumptive diagnosis of rsv infection can frequently be made on clinical grounds. confirmation can be made by viral culture, which may take 3 to 7 days. immunofluorescent assay (ifa) is a specific and sensitive test that can provide laboratory confirmation of rsv infection the same day. rapid diagnosis of rsv infection may have implications regarding prevention of nosocomial spread of rsv, early i ... | 1986 | 3524958 |
| occurrence of respiratory syncytial virus subtypes a and b strains in sweden. | the subtype characteristics of 22 strains of respiratory syncytial (rs) virus isolated in sweden were determined by the use of monoclonal antibodies. eleven antibodies specific for distinct epitopes on five different structural proteins were used in immunofluorescence and radioimmune precipitation assays. one group of 12 isolates were derived from a three-month epidemic during 1984, whereas the other ten virus isolates were recovered during a time period of 13 years (1971-1983). all isolates cou ... | 1986 | 3525747 |
| mycoplasma bovis infection in gnotobiotic calves and combined infection with respiratory syncytial virus. | mycoplasma bovis was inoculated alone or in combination with respiratory syncytial virus into the respiratory tracts of 12 gnotobiotic calves. clinical signs ranged from transient pyrexia to protracted fever accompanied by severe lower respiratory signs and in one case, arthritis. pulmonary lesions included foci of coagulative necrosis surrounded by mononuclear cells and suppurative bronchiolitis with varying degrees of lympho-reticular hyperplasia. no enhancement of lesions occurred in the comb ... | 1986 | 3535220 |
| common bacterial pneumonitis in infants. determining the etiology and tailoring the treatment. | in infantile pneumonia, we recommend close attention to the history and physical examination. baseline studies, including cbc, esr, blood cultures, and chest film, should be performed at onset and repeated as warranted. nasopharyngeal secretions or washings should be drawn by means of gentle suction and specimens sent for gram stain, fluorescent antibody stain for respiratory syncytial virus, and culture for bacteria and for viruses if possible. acute and convalescent serum specimens should be o ... | 1986 | 3511465 |
| diagnosis of bovine respiratory syncytial virus infections improved by virus detection in lung lavage samples. | the potential of lung lavage to diagnose and study infections with bovine respiratory syncytial virus (brsv) was investigated. the technique was simple to perform under field conditions, and even in calves with respiratory tract disease, serious drawbacks were not observed. in 9 epizootics of respiratory tract disease, brsv infection was diagnosed in 1 or more calves. the brsv was detected from a total of 21 of 32 calves by immunofluorescence in cells recovered from lavage fluids, and the virus ... | 1986 | 3511803 |
| detection of respiratory syncytial virus in nasopharyngeal secretions by a biotin-avidin elisa more sensitive than the fluorescent antibody technique. | the presence of respiratory syncytial virus (rsv) was investigated by immunofluorescent antibody (if) technique and by a biotin/avidin (ba) elisa in 156 samples of nasopharyngeal secretions (nps) obtained from infants and small children with acute respiratory disease. of 70 rsv-if-positive nps, 68 were positive by ba-elisa. of 86 rsv-if-negative nps, eight were positive by ba-elisa. ba-elisa could detect 0.5-1.0 ng rsv-protein. | 1986 | 3512768 |
| interleukin 1 and interleukin 1 inhibitor production by human macrophages exposed to influenza virus or respiratory syncytial virus. respiratory syncytial virus is a potent inducer of inhibitor activity. | respiratory viral infections are commonly associated with altered immune responses, such as proliferative responses to mitogens and antigens. to examine potential mechanisms, we examined production of il-1 and il-1 inhibitors by purified human peripheral blood-derived macrophages exposed to influenza virus or respiratory syncytial virus (rsv). il-1 and il-1 inhibitor activities in supernatant fluids from macrophages exposed to the viruses 24 h previously were measured using the standard mouse th ... | 1986 | 3485170 |
| passively acquired antibodies to respiratory syncytial virus impair the secondary cytotoxic t-cell response in the neonatal mouse. | passively acquired antibody has been known since the 1940s to impair the secondary antibody response to the homologous antigen. however, the effect of passive immunity on the t-cell response is largely unknown. the results presented here demonstrate that monoclonal antibodies (mabs) to respiratory syncytial virus (rsv), transferred in the mother's milk or injected directly, can reduce the generation of rsv-specific cytotoxic t-cell (tc) precursors by the neonatal mouse; the development of influe ... | 1986 | 3489669 |
| expression of the major glycoprotein g of human respiratory syncytial virus from recombinant vaccinia virus vectors. | the major glycoprotein, g, of human respiratory syncytial (rs) virus is a mr 84,000-90,000 species that has about 60% of its mass contributed by carbohydrate, most of which is in the form of o-linked oligosaccharides. the g protein contains neither a hydrophobic n-terminal signal sequence nor a hydrophobic c-terminal anchor region. instead, its amino acid sequence reveals only one region with significant hydrophobic character, which is between residues 38 and 66. in order to study the synthesis, ... | 1986 | 3455762 |
| nucleotide sequences for the gene junctions of human respiratory syncytial virus reveal distinctive features of intergenic structure and gene order. | complete sequences for the intergenic regions of the genome of human respiratory syncytial virus were obtained by dideoxynucleotide sequencing using synthetic oligonucleotides. these experiments established that the 10 respiratory syncytial viral genes are arranged, without additional intervening genes, in the order 3' 1c-1b-n-p-m-1a-g-f-22k-l 5'. for the first nine genes, the exact gene boundaries were identified by comparison of the genomic sequences with previously determined mrna sequences. ... | 1986 | 3460060 |
| neonatal respiratory syncytial virus infection: role of transplacentally and breast milk-acquired antibodies. | the effect of transplacentally and breast milk-acquired antibodies on respiratory syncytial virus infection was studied in neonatal and 2-month-old cotton rats. adult female rats infected intranasally with live virus regularly produced virus-specific antibodies in the serum, colostrum, and breast milk. by using foster feeding techniques, we showed that both transplacentally and breast milk-acquired antibodies were effective in reducing the replication of respiratory syncytial virus in the lungs ... | 1986 | 3512852 |
| administration of ribavirin to neonatal and pediatric patients during mechanical ventilation. | we have modified the circuits of pressure-preset and volume-preset ventilators to permit the administration of ribavirin to mechanically ventilated infants suffering from respiratory syncytial virus. the modifications isolate the ventilator itself and permit continuous aerosolization for as long as seven days without ventilator malfunction from the effects of crystallized medication. excessive spilling of ribavirin into the environment is also avoided. each institution must devise its own experi ... | 1986 | 10315718 |
| [method of spread of respiratory syncytial virus infection in a neonatology unit]. | 1985 | 3157115 | |
| respiratory syncytial virus and heart block--cause and effect? | a child aged 3 years who developed a respiratory syncytial virus infection is described. his admission was complicated by the development of an irregular pulse. he initially had a variable first or second degree heart block with transient electrocardiographic evidence suggestive of pericarditis and/or myocarditis and later developed complete heart block. he has remained asymptomatic for the past four years. a search for other causes of the heart block proved negative. it is tempting to suggest a ... | 1985 | 3859266 |
| seroprevalence of respiratory syncytial virus in free-ranging bighorn sheep. | sera from 187 (42%) of 447 free-ranging bighorn sheep (ovis canadensis) in 9 western states had antibody titers to respiratory syncytial virus (rsv). titers were from 1:5 to 1:640, as determined by the standard virus neutralization test. results of the survey indicated that rsv is widespread in free-ranging bighorn sheep populations and that rsv may be an important factor in the bighorn sheep pneumonia-complex. | 1985 | 3878355 |
| kinetics of synthesis of respiratory syncytial virus glycoproteins. | the synthesis of the two respiratory syncytial (rs) virus glycoproteins (vp66 and vp84) was examined under standard conditions and after treatment with tunicamycin and monensin. the protein backbone for vp66, the fusion protein (f1,2) is cotranslationally glycosylated to form f0, which is cleaved to form f1,2 by 20 min of chase. monensin treatment inhibited the cleavage of f0 over an 80 min chase period, indicating that this occurred late in the transit of f0 through the golgi apparatus or after ... | 1985 | 4031826 |
| respiratory syncytial virus infection in immunosuppressed animals: implications in human infection. | neonatal cotton rats were treated with cyclophosphamide parenterally for three weeks before intranasal inoculation of live respiratory syncytial virus (rsv). immunosuppressive therapy resulted in severe depletion of lymphocytes from the peripheral circulation, the spleen, and the thymus. in contrast to normal rats, immunosuppressed animals developed severe pulmonary pathology with marked infiltration of foamy macrophages. persistent degeneration and regeneration of bronchial epithelial cells wer ... | 1985 | 3908616 |
| [immunofluorescent demonstration of respiratory syncytial virus infection in calves]. | specific high-titre bovine conjugated antisera were obtained against respiratory syncytial virus. use was made of the reference strain nomi and the local isolate antonovo/448. the conjugates produced were shown to have equal qualities to those of the belgian conjugate used for comparison. the anti-antonovo/448 conjugated serum was used in the direct immunofluorescent method to demonstrate a respiratory syncytial virus antigen in organs of animals with acute respiratory diseases as well as to con ... | 1985 | 3909628 |
| [acetone-celloidin method for the cytological and immunofluorescent study of cell cultures infected with various viruses]. | a method was worked out for the extraction of a cell monolayer from test tubes with the use of 10 per cent acetone solution of celloidin. this made it possible to carry out parallel cytologic and immunofluorescent studies of cultures infected with various viruses. in contrast to the alcohol-ether celloidin used in histology the use of the acetone solution of celloidin, suggested by us, preserved to a larger extent the viruses of different structure and composition, and, therefore, was shown to b ... | 1985 | 3909629 |
| [structural aspect of the respiratory syncytial virus genome]. | 1985 | 3914773 | |
| [clinical aspects of acute and chronic respiratory syncytial virus infection in adult polyclinic patients]. | a total of 224 workers and employees engaged at the smolensk flax spinning plant, suffering from acute respiratory diseases, were examined on an outpatient basis. virus etiology of the diseases was established in 84 patients (37.7%) with the aid of the serological tests (hi and cf) and immunofluorescent techniques. respiratory syncytial virus infection was diagnosed in 19 patients (8.5%). as regards the clinical picture the symptoms of intoxication were unmarked. half the patients demonstrated m ... | 1985 | 3983830 |
| [experimental infection of lambs with strains of the bovine respiratory syncytial virus]. | lambs were successfully infected with bovine strains of a respiratory syncytial virus. when the nomi reference strain and the local antonovo strain of the rsv were used i/v and via the trachea the animals reacted on the 3rd to 5th day with dizziness, dyspnea, conjunctivitis, nasal discharges, etc., more strongly manifested with the use of the antonovo strain. the temperature reaction started on the 3rd day and receded on the 7th day, with a second peak on the 8h day. serologically, rsv-antibodie ... | 1985 | 3992914 |
| development of cell-mediated cytotoxic activity in the respiratory tract after experimental infection with respiratory syncytial virus. | the development of natural killer cell and other antibody-independent cellular cytotoxic response to rsv were studied in splenic and pulmonary mononuclear effector cells obtained from groups of 6-week-old cotton rats after subcutaneous (sc) or intranasal (in) immunization with live virulent or ultra-violet (uv) inactivated respiratory syncytial virus (rsv). no virus-induced cytotoxic activity was observed after sc immunization with live virus or in inoculation of inactivated non-infectious virus ... | 1985 | 3899431 |
| the production of monoclonal antibodies against respiratory syncytial virus and its clinical applications. | the availability of monoclonal antibodies against various viral components, either internal nucleoproteins or membrane proteins, has greatly advanced our understanding of the biochemistry of respiratory syncytial virus (rsv). the components responsible for serum neutralization have been identified by monoclonal antibodies, thus facilitating the development of synthetic rsv vaccine. a number of clinical tests have been devised using monoclonal antibodies for either qualitative or quantitative det ... | 1985 | 3899483 |
| rapid detection of respiratory syncytial virus in nasopharyngeal secretions by enzyme-linked immunosorbent assay. | a new enzyme-linked immunosorbent assay (elisa) respiratory syncytial virus antigen detection kit (ortho diagnostics, inc., raritan, n.j.) was compared with virus culture and with the indirect fluorescent antibody test (fat) by using fresh nasal washings from children with suspected respiratory syncytial virus infection. both uncentrifuged nasal washings and pellets from centrifuged split specimens were examined by elisa. the elisa was considered positive when the optical density was greater tha ... | 1985 | 3902879 |
| etiology of community-acquired pneumonia in patients requiring hospitalization. | the etiology of community-acquired pneumonia was studied in 127 patients with roentgenologically verified pneumonia who needed hospitalization. etiology was determined on the basis of a positive blood culture and/or a significant antibody titer increase. streptococcus pneumoniae was the probable etiological agent in 69 patients, nontypeable haemophilus influenzae in five patients, streptococcus pyogenes in two patients, and legionella pneumophila and staphylococcus aureus in one patient each. ev ... | 1985 | 4018066 |
| ribavirin treatment of respiratory syncytial viral infection in infants with underlying cardiopulmonary disease. | aerosolized ribavirin was evaluated in the treatment of respiratory syncytial virus lower respiratory tract disease in 53 infants, 36 of whom had underlying diseases. of the total infants, 26 were studied in a double-blind, placebo-controlled manner; 14 received ribavirin and 12 received placebo, a water aerosol, for an average of five days. when the infants with bronchopulmonary dysplasia and congenital heart disease treated with ribavirin were compared with those receiving placebo, the treated ... | 1985 | 3903239 |
| immunoprophylaxis and immunotherapy of respiratory syncytial virus infection in the cotton rat. | human convalescent antiserum to respiratory syncytial virus (rsv) administered intraperitoneally to cotton rats prior to rsv challenge provided near-complete protection from pulmonary infection. antiserum given subsequent to viral challenge reduced pulmonary viral titers 100-fold or greater within 24 h. sandoglobulin, a preparation of purified human igg with high titer of anti-rsv neutralizing activity, produced the same effects as convalescent antiserum. sandoglobulin was absorbed rapidly and p ... | 1985 | 3907188 |
| calf pneumonia. | infectious calf pneumonia is a high-mortality pneumonia of housed dairy-type calves. viruses are important etiologic agents and infection with bovine respiratory syncytial virus (rsv) and parainfluenza type 3 virus (pi-3 virus) may result in extensive, and sometimes fatal, lung damage. respiratory viral infections are frequently followed by mycoplasmal and secondary bacterial invasion of the lower respiratory tract, which increases the extent and severity of lung damage. bad housing, particularl ... | 1985 | 3907774 |
| bovine respiratory syncytial virus. | the current knowledge is reviewed in regards to the importance of bovine respiratory syncytial virus in the bovine respiratory disease complex. the epidemiology, clinical disease, pathologic findings, pathogenesis, diagnosis, treatment, and prevention of this viral disease are discussed. | 1985 | 3907775 |
| outbreak of respiratory syncytial virus in france. | a report is given of an outbreak of respiratory syncytial virus infection in a neonatal unit in france. twenty-three of 32 infants were infected (72%) despite infection control procedures. prophylactic administration of non-specific gamma globulins was associated with a significant decrease in infection rate (p less than 0.05). the administration of transfer factor to infected infants was also associated with a significantly lower rate of severe respiratory diseases (p less than 0.05). | 1985 | 4043060 |
| comparison of ortho respiratory syncytial virus enzyme-linked immunosorbent assay and hep-2 cell culture. | two hundred seventy nasopharyngeal aspirates were tested in duplicate with the ortho diagnostics, inc. (raritan, n.j.), respiratory syncytial virus antigen enzyme-linked immunosorbent assay. the test was sensitive (80 to 82%) and specific (96%) when compared with cell culture. the enzyme-linked immunosorbent assay detected seven antigen-positive specimens not among the 71 specimens that were positive for respiratory syncytial virus in cell culture. a blocking test confirmed those specimens as tr ... | 1985 | 4066920 |
| human mononuclear phagocytic cell interaction with some paramyxoviridae. | in consideration of the peculiar relationship between paramyxoviridae and immunocompetent system, we studied interactions of measles virus, newcastle-disease virus and respiratory syncytial virus with human monocytes and macrophages differentiated "in vitro". mononuclear cells were challenged with three different paramyxovirus at different multiplicities of infection. infected cells were then checked for expression of specific viral antigens by immunofluorescent test (fa). moreover, we have eval ... | 1985 | 3916331 |
| respiratory syncytial virus polypeptides. iv. the oligosaccharides of the glycoproteins. | the cell-associated glycoproteins of respiratory syncytial (rs) virus included gp1 (90k), vp70 (70k), vgp48 (48k) and gp26 (26k). although present in infected cells, there was no vp70 in purified virus. trypsin treatment of infected cells removed 80 to 90% of vp70 as well as its products vgp48 and gp26. this suggested that most of the vp70 in the cell is located on the plasma membrane. the glycoproteins of purified rs virus (gp1, vgp48 and gp26) contain mannose, galactose and fucose as well as g ... | 1985 | 3919149 |
| characterization of the 10 proteins of human respiratory syncytial virus: identification of a fourth envelope-associated protein. | a total of 13 respiratory syncytial (rs) virus specific polypeptides were identified by pulse-chase metabolic labeling of infected hep-2 cells. ten of the 13 proteins were shown to be unique. they were the l, g, f (f1, f2), n, p, m, 24k, 14k, 11k and 9.5k proteins. these conclusions were based on peptide mapping and on previous work showing that each of 10 polypeptides are coded for by a unique mrna. the seven largest proteins, l, g, f (f1, f2), n, p, m and 24k were identified clearly as virion ... | 1985 | 3993233 |
| experimental bovine respiratory syncytial virus infection in conventional calves: light microscopic lesions, microbiology, and studies on lavaged lung cells. | conventionally raised male holstein calves, 1 month of age, were infected by intranasal and intratracheal inoculation with bovine respiratory syncytial virus. viral antigen was identified by fluorescence microscopy most commonly in the cytoplasm of tracheal and bronchial epithelial cells 3 to 5 days after inoculation. cytoplasmic viral antigen was identified also in nasal, nasopharyngeal, bronchiolar, and alveolar epithelial cells and in alveolar macrophages. bronchitis and tracheitis, character ... | 1985 | 3994121 |
| respiratory syncytial virus-specific ige responses following infection: evidence for a predominantly mucosal response. | in order to determine whether ige production occurs predominantly at mucosal or systemic sites, we studied the production of respiratory syncytial virus (rsv)-specific antibody in serum and nasopharyngeal secretions (nps) from 41 patients with rsv infection using an enzyme-linked immunosorbent assay. rsv-ige was found in higher titer in samples of nps than in simultaneously obtained serum specimens at all phases of illness. despite the excess dilution incurred in the collection process, rsv-ige ... | 1985 | 4000767 |
| respiratory syncytial virus polypeptides. v. the kinetics of glycoprotein synthesis. | the cell-associated glycoproteins of respiratory syncytial virus included gp1 (90k), vp70 (70k), vgp48 (48k) and gp26 (26k). the time course of virus protein synthesis in hela cells revealed that the nucleocapsid protein (vpn41) was the first to appear at 11 h post-infection followed by the appearance of the other viral proteins at 16 h post-infection. pulse-chase experiments with [3h]leucine or [35s]methionine demonstrated that the precursor for vgp48 and gp26 was an uncleaved protein of 70k (v ... | 1985 | 4009147 |
| mrna sequence of three respiratory syncytial virus genes encoding two nonstructural proteins and a 22k structural protein. | an mrna sequence of two human respiratory syncytial viral nonstructural protein genes and of a gene for a 22,000-molecular-weight (22k) protein was obtained by cdna cloning and dna sequencing. sequences corresponding to the 5' ends of the respective transcripts were deduced directly by primer extension and dideoxy nucleotide sequencing of the mrnas. the availability of a bicistronic clone (prsc6) confirmed the gene order for this portion of the genome. contrary to other unsegmented negative-stra ... | 1985 | 4009789 |
| [bovine respiratory syncytial virus (brsv) in switzerland: a serologic study]. | 1985 | 4071020 | |
| experimental bovine respiratory syncytial virus infection in conventional calves: ultrastructural respiratory lesions. | the morphogenesis and repair of airway and alveolar injury induced by bovine respiratory syncytial virus (brsv) was studied ultrastructurally in conventional calves to characterize pulmonary cell types susceptible to viral infection and cytopathologic changes associated with infection. viral nucleocapsids and budding virions were present in tracheal and bronchial ciliated and nonciliated epithelial cells and mucous cells 3, 5, and 7 days after inoculation and in bronchiolar ciliated and noncilia ... | 1985 | 3994122 |
| lobar collapse with respiratory syncytial virus pneumonitis. | in a study of 30 children with uncomplicated respiratory syncytial virus (rsv) pneumonias, a high incidence of lobar collapse (8/30-26%) was noted. this involved the right upper lobe in seven patients and the left upper lobe in one patient. it is probably attributable to anatomical predispositions, sloughing of necrotic epithelium, and stimulation of mucus production. lobar collapse should be considered part of the spectrum of rsv pneumonitis. | 1985 | 4000751 |
| severe respiratory syncytial virus infection: clinical features, nosocomial acquisition and outcome. | respiratory syncytial virus (rsv) frequently causes bronchiolitis in young infants. rsv infection was established by culture, serologic response, immunofluorescence of secretions or lung tissue or typical histology on pathologic examination of the lungs in 10 patients who were hospitalized at children's medical center, dallas. eight were infants 4 days to 3 months old, one was 9 months and one was 16 months old. seven patients were afebrile but had rapidly progressive diffuse infiltrates on ches ... | 1985 | 4000987 |
| comparison of a newly developed enzyme-linked immunosorbent assay with complement fixation and neutralisation tests for serology of bovine respiratory syncytial virus infections. | an indirect double antibody sandwich enzyme-linked immunosorbent assay (elisa) was developed for the detection and titration of serum antibodies to bovine respiratory syncytial virus (brsv). the elisa was compared with a complement fixation (cf) test and a test for virus neutralising antibody in serum (virus neutralisation [vn] test). testing sera collected in dairy herds revealed the closest correlation between the results of the elisa and the cf test with respect to brsv antibody titres. the v ... | 1985 | 4012035 |
| an outbreak of respiratory syncytial virus infection in a continuing-care geriatric ward. | a small outbreak of respiratory syncytial virus infection in a continuing-care geriatric ward during the period december 1982 to march 1983 is reported. it demonstrates that respiratory syncytial virus infection can cause serious lower respiratory tract infection even in patients without any previous history of chest disease. | 1985 | 4013906 |
| respiratory tract infections. goals for 1995. | goals to be identified for 1995, a decade hence, in the prevention, diagnosis, and management of respiratory tract infections may conveniently be divided into diagnostic goals and goals in therapy and prophylaxis. major diagnostic goals for bacterial, viral, and mycoplasmal infections of the respiratory tract focus on the development of systems to identify microbial components, such as specific antigens or segments of dna, using monoclonal antibody techniques or dna probes for hybridization. spu ... | 1985 | 4014289 |
| serologic studies of bovine respiratory syncytial virus in minnesota cattle. | serum antibody titers to bovine respiratory syncytial virus were determined for 559 cattle. serum samples were obtained through the minnesota state-federal brucellosis laboratory and were collected over a 1-year period. results of this study revealed an antibody prevalence of 65.5% to bovine respiratory syncytial virus. the distribution of antibody titers is presented, as well as analysis of titers based on breed, sex, and age of the cattle. | 1985 | 4014838 |