Publications

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the effects of potato virus y-derived virus small interfering rnas of three biologically distinct strains on potato (solanum tuberosum) transcriptome.potato virus y (pvy) is one of the most economically important pathogen of potato that is present as biologically distinct strains. the virus-derived small interfering rnas (vsirnas) from potato cv. russet burbank individually infected with pvy-n, pvy-ntn and pvy-o strains were recently characterized. plant defense rna-silencing mechanisms deployed against viruses produce vsirnas to degrade homologous viral transcripts. based on sequence complementarity, the vsirnas can potentially degrade host ...201728716126
a novel recombined potato virus y isolate in china.this study reports the findings of a distinct potato virus y (pvy) isolate found in northeast china. one hundred and ten samples (leaves and tubers) were collected from potato plants showing mosaic symptoms around the city of harbin in heilongjiang province of china. the collected tubers were planted and let to grow in a greenhouse. new potato plants generated from these tubers showed similar symptoms, except for one plant. subsequent serological analyses revealed pvy as the causing agent of the ...201728811755
breeding of a new potato variety 'nagasaki kogane' with high eating quality, high carotenoid content, and resistance to diseases and pests.'nagasaki kogane' is a new potato variety bred from a cross between 'saikai 35' as a female parent and 'saikai 33' as a male. 'saikai 35' is resistant to bacterial wilt, contains the h1 and rychc genes for resistance to the potato cyst nematode (pcn) and potato virus y (pvy), respectively, and has high carotenoid content, while 'saikai 33' has large and high-yielding tubers and is resistant to both bacterial wilt and pcn. the carotenoid content of 'nagasaki kogane' is higher than that of 'dejima ...201728744186
the phylogenetics of the global population of potato virus y and its necrogenic recombinants.potato virus y (pvy) is a major pathogen of potatoes and other solanaceous crops worldwide. it is most closely related to potyviruses first or only found in the americas, and it almost certainly originated in the andes, where its hosts were domesticated. we have inferred the phylogeny of the published genomic sequences of 240 pvy isolates collected since 1938 worldwide, but not the andes. all fall into five groupings, which mostly, but not exclusively, correspond with groupings already devised u ...201728458913
coilin, the signature protein of cajal bodies, differentially modulates the interactions of plants with viruses in widely different taxa.cajal bodies (cbs) are distinct nuclear bodies physically and functionally associated with the nucleolus. in addition to their traditional function in coordinating maturation of certain nuclear rnas, cbs participate in cell cycle regulation, development, and regulation of stress responses. a key "signature" component of cbs is coilin, the scaffolding protein essential for cb formation and function. using an rna silencing (loss-of-function) approach, we describe here new phenomena whereby coilin ...201724637832
development of molecular markers tightly linked to pvr4 gene in pepper using next-generation sequencing.it is imperative to identify highly polymorphic and tightly linked markers of a known trait for molecular marker-assisted selection. potyvirus resistance 4 (pvr4) locus in pepper confers resistance to three pathotypes of potato virus y and to pepper mottle virus. we describe the use of next-generation sequencing technology to generate molecular markers tightly linked to pvr4. initially, comparative genomics was carried out, and a syntenic region of tomato on chromosome ten was used to generate p ...201725798050
rnai-mediated simultaneous resistance against three rna viruses in potato.rna interference (rnai) technology has been successfully applied in stacking resistance against viruses in numerous crop plants. during rnai, the production of small interfering rnas (sirnas) from template double-standard rna (dsrna) derived from expression constructs provides an on-switch for triggering homology-based targeting of cognate viral transcripts, hence generating a pre-programmed immunity in transgenic plants prior to virus infection. in the current study, transgenic potato lines (so ...201728194691
virus surveys of capsicum spp. in the republic of benin reveal the prevalence of pepper vein yellows virus and the identification of a previously uncharacterised polerovirus species.surveys were conducted in 2014 and 2015 in southern and northern benin, respectively, to identify the viruses infecting peppers (capsicum spp.). the samples were screened by elisa for cucumber mosaic virus (cmv), pepper veinal mottle virus (pvmv), potato virus y (pvy) and tomato yellow leaf curl virus (tylcv). a generic reverse transcription pcr (rt-pcr) was used to test for the presence of poleroviruses. elisa tests confirmed the prevalence of all viruses, while the rt-pcr detected pepper vein ...201728220324
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