Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| rna helicase activity of semliki forest virus replicase protein nsp2. | semliki forest virus replicase protein nsp2 shares sequence homology with several putative ntpases and rna helicases. nsp2 has rna-dependent ntpase activity. here we expressed polyhistidine-tagged nsp2 in escherichia coli, purified it by metal-affinity chromatography, and used it in rna helicase assays. rna helicase ci of plum pox potyvirus was used as a positive control. unwinding of alpha-32p-labelled partially double-stranded rna required nsp2, mg2+ and ntps. nsp2 with a mutation, k192n, in t ... | 1999 | 10217401 |
| use of parvovirus-like particles for vaccination and induction of multiple immune responses. | expression of the vp2 gene of autonomous parvoviruses in insect cells with the use of the baculovirus system has led to the production of virus-like particles (vlps) formed by the self-assembly of vp2. these vlps are expressed at high levels and can easily be purified by salt fractionation. they are highly immunogenic in the corresponding host, being fully protective at doses as low as 1-2 microg of purified material per animal. no special adjuvants are required. an interesting property of these ... | 1999 | 10075910 |
| detection of porcine parvovirus dna by the polymerase chain reaction assay using primers to the highly conserved nonstructural protein gene, ns-1. | porcine parvovirus (ppv) infection is associated with reproductive losses in swine and its causative agent, the ppv, has been isolated worldwide. serological surveys and virus isolation studies throughout brazil confirm the occurrence of ppv infection in this country. the most common methods to detect ppv infection are fluorescent antibody staining of fetal tissues, hemagglutination assay of tissue extracts and virus isolation from fetal tissues. non-specificity and low sensitivity are the major ... | 1999 | 10204709 |
| intranasal delivery of recombinant parvovirus-like particles elicits cytotoxic t-cell and neutralizing antibody responses. | we previously demonstrated that chimeric porcine parvovirus-like particles (ppv:vlp) carrying heterologous epitopes, when injected intraperitoneally into mice without adjuvant, activate strong cd4(+) and cd8(+) t-cell responses specific for the foreign epitopes. in the present study, we investigated the immunogenicity of ppv:vlp carrying a cd8(+) t-cell epitope from the lymphocytic choriomeningitis virus (lcmv) administered by mucosal routes. mice immunized intranasally with recombinant ppv:vlp, ... | 1999 | 10074120 |
| monoclonal antibodies to the gp5 of porcine reproductive and respiratory syndrome virus are more effective in virus neutralization than monoclonal antibodies to the gp4. | the arterivirus porcine reproductive and respiratory syndrome virus (prrsv) contains six structural proteins the roles of which are not completely understood. in a preceding study, immunization with the dutch isolate i10 of prrsv had led to the development of mabs against four structural proteins [wieczorek-krohmer, m., 1994. herstellung und charakterisierung von monoklonalen antikörpern gegen das virus des porzinen reproduktiven und respiratorischen syndroms (prrsv). inaugural-dissertation, lud ... | 1999 | 10227120 |
| new device and method for capture, reverse transcription and nested pcr in a single closed-tube. | a device and improved method based on the use of a compartmentalized eppendorf tube that allows capture, reverse transcription and nested-pcr in a single closed-tube has been developed and patented. the main advantages of the system are the high sensitivity obtained, the simplicity, the low risk of contamination and the easy establishment of adequate conditions for nested-pcr. the method has been successfully applied to the detection and characterization of citrus tristeza closterovirus and plum ... | 1999 | 10037824 |
| mitotic stability of infection-induced resistance to plum pox potyvirus associated with transgene silencing and dna methylation. | plum pox potyvirus (ppv) infection of transgenic nicotiana benthamiana plants that expressed the ppv nib rna replicase carrying a gly to val mutation at the gdd motif (nibv lines) induced a phenotype of virus resistance and transgene silencing, which was not transmissible to the progeny after self-fertilization (h. s. guo and j. a. garcía, mol. plant-microbe interact. 10:160-170, 1997). here, we demonstrate that the induced resistance of nibv plants is mitotically stable after plant propagation ... | 1999 | 9926412 |
| reproduction of lesions of postweaning multisystemic wasting syndrome in gnotobiotic piglets. | neonatal gnotobiotic piglets were inoculated with tissue homogenates and low- and high-passage cell culture material to determine if the lesions of the newly described porcine postweaning multisystemic wasting syndrome (pmws) could be reproduced. for this, 17 3-day-old gnotobiotic piglets were inoculated intranasally with pelleted chloroform-treated, filtered extracts from cell cultures, filter-sterilized homogenates of lymphoid tissue from pmws-affected piglets, or control materials. piglets we ... | 1999 | 9925205 |
| virus safety of avital bone tissue transplants: evaluation of sterilization steps of spongiosa cuboids using a peracetic acid-methanol mixture. | the aim of this study was to validate the virus-inactivating/eliminating capacity of the manufacturing process of spongiosa cuboids. both the sterilization step with peracetic acid (paa)/ethanol and the defatting step of bones with chloroform/methanol (2:1, v/v) were investigated. relevant enveloped, non-enveloped, and model viruses belonging to different virus families were included in the investigation: human immunodeficiency virus type 2 (hiv-2), hepatitis a virus (hav), poliovirus (pv-1), ps ... | 1999 | 10652175 |
| preparation of diagnostic monoclonal antibodies against two potyviruses. | diagnostic monoclonal and polyclonal antibodies against bean yellow mosaic virus (bymv) and plum pox virus (ppv) were prepared, characterized and used for detection of these viruses in infected plants by enzyme-linked immunosorbent assay (elisa), immunoblot analysis and tissue print immunoblot assay (tpiba). | 1999 | 10749372 |
| plum pox virus capsid protein mobility in sds-polyacrylamide gel electrophoresis. | slovak plum pox virus (ppv) isolates bor-3 and kr-4 behaved atypically in polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (sds-page) of their capsid proteins (cps). whereas other tested ppv isolates could be sorted by sds-page into groups corresponding to the m or d strains, as controlled by restriction fragment length polymorphism (rflp) analysis, the two above mentioned isolates behaved as the m strain according to rflp analysis but not sds-page. slight mobility di ... | 1999 | 10749373 |
| engineering parvovirus-like particles for the induction of b-cell, cd4(+) and ctl responses. | an antigen delivery system based on hybrid recombinant parvovirus-like particles (vlps) formed by the self-assembly of the capsid vp2 protein of porcine (ppv) or canine parvovirus (cpv) expressed in insect cells with the baculovirus system has been developed. ppv:vlps containing a cd8(+) epitope from the lcmv nucleoprotein evoked a potent ctl response and were able to protect mice against a lethal infection with the virus. also, ppv:vlps containing the c3:t epitope from poliovirus elicited a cd4 ... | 1999 | 10506659 |
| comparative analysis of dna flow cytometry and cytology of bladder washings: review of discordant cases. | dna analysis is becoming an important diagnostic and prognostic adjunct test in urinary cytology. the aim of this study was to compare the results of dna flow cytometry (fcm) with the cytologic diagnosis of bladder washings (bw). dna ploidy was evaluated in 251 bw. in 65 cases, follow-up surgical biopsies were available. cytology results were classified as positive and negative, and fcm results were categorized as diploid and aneuploid. both tests were evaluated independently. cases were defined ... | 2000 | 10649514 |
| biotechnological aspects of plum pox virus. | plum pox potyvirus (ppv), the causal agent of a devastating disease that affects stone fruit trees, is becoming a target of intense studies intended both to fight against viral infection and to develop practical applications based on the current knowledge of potyvirus molecular biology. this review focuses on biotechnological aspects related to ppv, such as novel diagnostic techniques that facilitate detection and typing of virus isolates, strategies to implement pathogen-derived resistance thro ... | 2000 | 10656327 |
| identification of a pathogenicity determinant of plum pox virus in the sequence encoding the c-terminal region of protein p3+6k(1). | a full-length genomic cdna clone of a plum pox potyvirus (ppv) isolate belonging to the m strain (ppv-ps) has been cloned downstream from a bacteriophage t7 polymerase promoter and sequenced. transcripts from the resulting plasmid, pgppvps, were infectious and, in herbaceous hosts, produced symptoms that differed from those of virus progeny of pgppv, a full-length genomic cdna clone of the d strain ppv-r. viable ppv-r/-ps chimeric viruses were constructed by recombination of the cdna clones in v ... | 2000 | 10675393 |
| mutations in the coat protein gene of plum pox virus suppress particle assembly, heterologous encapsidation and complementation in transgenic plants of nicotiana benthamiana. | two different motifs in the coat protein (cp) of plum pox virus (ppv) (r(3015)q(3016), d(3059)) were mutated by replacing the respective amino acids with others possessing different chemical properties. the mutated cp genes were introduced into an infectious full-length clone of ppv (p35ppv-nat) to investigate their influence on systemic infection of transgenic wild-type ppv cp-expressing and non-transgenic plants of nicotiana benthamiana. all mutants failed to establish systemic infections in n ... | 2000 | 10675394 |
| trial-of-antibiotic algorithm for the diagnosis of tuberculosis in a district hospital in a developing country with high hiv prevalence. | to evaluate a diagnostic algorithm for pulmonary tuberculosis based on smear microscopy and objective response to trial of antibiotics. | 2000 | 10864181 |
| transgenic or plant expression vector-mediated recombination of plum pox virus. | different mutants of an infectious full-length clone (p35ppv-nat) of plum pox virus (ppv) were constructed: three mutants with mutations of the assembly motifs rq and df in the coat protein gene (cp) and two cp chimeras with exchanges in the cp core region of zucchini yellow mosaic virus and potato virus y. the assembly mutants were restricted to single infected cells, whereas the ppv chimeras were able to produce systemic infections in nicotiana benthamiana plants. after passages in different t ... | 2000 | 10906199 |
| occupational risk of infection by varicella zoster virus in belgian healthcare workers: a seroprevalence study. | to assess the prevalence of varicella zoster virus (vzv) antibodies in flemish (belgian) healthcare workers, to investigate the association between seronegativity and selected variables, and to assess the reliability of recall about disease as a predictor of immunity. | 2000 | 10935943 |
| the use of transgenic fruit trees as a resistance strategy for virus epidemics: the plum pox (sharka) model. | sharka or plum pox, caused by plum pox virus (ppv: genus potyvirus; family potyviridae), is the most serious disease of prunus. most cultivated prunus species are highly susceptible and conventional breeding has not produced highly resistant and commercially acceptable varieties. success in developing virus-resistant herbaceous crops through genetic engineering led us to investigate this approach for resistance to ppv. our programme aims to develop a biotechnological approach to ppv control that ... | 2000 | 11137162 |
| construction of a stable and highly infectious intron-containing cdna clone of plum pox potyvirus and its use to infect plants by particle bombardment. | an infectious plum pox potyvirus cdna clone was constructed placing a copy of the full-length sequence of the virus genome between an enhanced cauliflower mosaic virus 35s promoter and a nopaline synthase termination signal. stabilization of the clone and faster growth of bacteria, in addition to higher plasmid yield, followed a modification consisting of the insertion of an intron which interrupted the viral open reading frame at the p3 region. this intron-containing clone was infectious when i ... | 2000 | 10958981 |
| preservation of 5'-end integrity of a potyvirus genomic rna is not dependent on template specificity. | full-length in vitro transcripts of plum pox potyvirus (ppv) genomic rna with mutations altering the number of 5'-terminal adenosine residues were able to infect nicotiana clevelandii plants, whereas a mutant with a substitution of adenosine in position 2 by guanosine failed to infect. the genomic 5' end was template-independently repaired during in vivo rna synthesis producing wild-type viral progeny. putative models of replication initiation are discussed. | 2000 | 10753716 |
| a sequential study of experimental infection of pigs with porcine circovirus and porcine parvovirus: immunostaining of cryostat sections and virus isolation. | the sequential tissue distribution of virus was investigated using virus isolation and immunofluorescence tests in 1-day-old piglets inoculated with porcine circovirus 2 (pcv2) and/or porcine parvovirus (ppv). enlarged mesenteric lymph nodes were seen in the pig inoculated with pcv2 alone and killed at 26 days post-inoculation (pi). one of the pigs inoculated with pcv2 and ppv and killed at 21 days pi had an enlarged liver. the pig killed at 26 days pi in this group had enlarged liver, kidneys a ... | 2000 | 10763376 |
| detection of human cytomegalovirus pp67 late gene transcripts in cerebrospinal fluid of human immunodeficiency virus type 1-infected patients by nucleic acid sequence-based amplification. | this study examined the clinical correlation between the presence of human cytomegalovirus (hcmv) pp67 mrna in cerebrospinal fluid (csf) and active hcmv central nervous system (cns) disease in patients with human immunodeficiency virus type 1 (hiv-1). in total, 76 csf specimens collected from 65 hiv-1-positive patients diagnosed with hcmv cns disease, other non-hcmv-related cns diseases, or no cns disease were tested for the presence of hcmv pp67 mrna using the nuclisens cytomegalovirus (cmv) pp ... | 2000 | 10790122 |
| viral wasting syndrome of swine: experimental reproduction of postweaning multisystemic wasting syndrome in gnotobiotic swine by coinfection with porcine circovirus 2 and porcine parvovirus. | one-day-old gnotobiotic piglets were inoculated intranasally with in vitro passaged porcine circovirus 1 (pcv-1), pcv-2, and porcine parvovirus (ppv) alone or in combination (pcv-1/pcv-2, pcv-1/ppv, and pcv-2/ppv). piglets were evaluated for 1) the development of porcine postweaning multisystemic wasting syndrome (pmws), 2) distribution of viral antigens by immunochemistry, and 3) viremia and the presence of viral dna in nasal and ocular secretions and feces. all single agent-infected piglets an ... | 2000 | 10810990 |
| episomal expression of a hammerhead ribozyme directed against plum pox virus. | two related antisense rnas directed against plum pox virus (ppv) were expressed episomally in nicotiana clevelandii by infection with recombinant potato virus x (pvx). one recombinant pvx expressed an ordinary ppv antisense rna of about 400 nucleotides, while the other expressed a related antisense rna that carried the catalytic domain of a hammerhead ribozyme. inoculation with the latter recombinant pvx resulted in the accumulation of ribozyme rna that was catalytically active when tested in vi ... | 2000 | 10930659 |
| generation of recombinant parapoxviruses: non-essential genes suitable for insertion and expression of foreign genes. | orf virus (ov) is an epitheliotropic poxvirus and belongs to the genus parapoxvirus (ppv). ppv, especially ov, is regarded as a promising candidate for an expression vector. among available live vaccines only strain d1701 represents a highly attenuated ov strain with clearly reduced pathogenicity. therefore, we started to identify potentially non-essential genes or regions of d1701, which might be suitable for insertion and expression of foreign genes. the present contribution reviews some of th ... | 2000 | 11000469 |
| effect of different baculovirus inactivation procedures on the integrity and immunogenicity of porcine parvovirus-like particles. | we have demonstrated earlier the usefulness of recombinant porcine parvovirus (ppv) virus-like particles (vlps) as an efficient recombinant vaccine for ppv. here, we have demonstrated that preparations of ppv vlps could be contaminated by recombinant baculoviruses. since these baculoviruses can be a problem for the registration and safety requirements of the recombinant vaccine, we have tested different baculovirus inactivation strategies, studying simultaneously the integrity and immunogenicity ... | 2000 | 11115693 |
| the effect of porcine parvovirus and porcine reproductive and respiratory syndrome virus on porcine reproductive performance. | from a worldwide perspective, porcine parvovirus (ppv) and porcine reproductive and respiratory syndrome virus (prrsv) are the most common viral causes of porcine reproductive failure. a typical epidemic of ppv-induced reproductive failure is presented as an increased number of mummified fetuses and sometimes, entire litters are mummified. if infection with ppv is very early in gestation, the number of liveborn pigs may be further reduced as a result of embryonic death and resorption. during the ... | 2000 | 10844195 |
| experimental infection of colostrum deprived piglets with porcine circovirus 2 (pcv2) and porcine reproductive and respiratory syndrome virus (prrsv) potentiates pcv2 replication. | experimental infection of colostrum-deprived (cd) pigs with a combined inoculum of porcine circovirus 2 (pcv2) and porcine reproductive and respiratory syndrome virus (prrsv) potentiated the replication and distribution of pcv2 virus, when compared with pigs inoculated with pcv2 alone. the replication and distribution of prrsv in dually infected pigs was not enhanced, when compared to pigs inoculated with prrsv alone. the mechanisms involved in the potentiation of pcv2 replication in pcv2/prrsv ... | 2000 | 11205128 |
| native electrophoresis and western blot analysis (neweb): a method for characterization of different forms of potyvirus particles and similar nucleoprotein complexes in extracts of infected plant tissues. | a combination of native electrophoresis and immunodetection (western blot) was used for the characterization of nucleoprotein particles of the potyvirus plum pox virus (ppv). virus particles were electrophoresed directly from plant extracts in agarose or mixed acrylamide-agarose gels under native conditions, blotted on nitrocellulose membranes, and characterized with the aid of a coat protein-specific antibody. using this combined methodology, called neweb (native electrophoresis and western blo ... | 2000 | 10950988 |
| in vivo induction of a high-avidity, high-frequency cytotoxic t-lymphocyte response is associated with antiviral protective immunity. | many approaches are currently being developed to deliver exogenous antigen into the major histocompatibility complex class i-restricted antigen pathway, leading to in vivo priming of cd8(+) cytotoxic t cells. one attractive possibility consists of targeting the antigen to phagocytic or macropinocytic antigen-presenting cells. in this study, we demonstrate that strong cd8(+) class i-restricted cytotoxic responses are induced upon intraperitoneal immunization of mice with different peptides, chara ... | 2000 | 10846055 |
| localization of plum pox virus in stem and petiole tissues of apricot cultivars by immuno-tissue printing. | localization of plum pox virus (ppv) in stem and petiole tissues of four susceptible and four resistant apricot cultivars has been studied. consecutive 1-mm spaced transverse sections were taken from the tissues and were printed onto nitrocellulose membrane in duplicate. for virus-specific detection, one series of prints was probed with an antibody to ppv coat and the density of stains was evaluated by light microscopy. another series of prints was treated with a substrate but not with the antib ... | 2000 | 11332274 |
| efficiency of inoculation of peach gf305 seedlings with plum pox virus by different methods. | peach gf305 is frequently used as rootstock in experiments to evaluate the resistance of different species of prunus to plum pox virus (ppv) because of its extreme susceptibility. however, transmission of ppv in prunus species is sometimes problematic due to its low concentration or uneven distribution in these species. to determine the most effective way of transmitting the virus, different infection methods (by aphids, grafting, mechanical infection and injection) were tested using dideron ppv ... | 2000 | 11332275 |
| sensitivity, specificity, and positive predictive value of oral opportunistic infections in adults with hiv/aids as markers of immune suppression and viral burden. | the purpose of this study was to assess the use of human immunodeficiency virus (hiv)-related oral opportunistic infections as markers of immune suppression and viral burden in adults with hiv/acquired immunodeficiency syndrome (aids). | 2000 | 10936837 |
| budding of fowlpox and pigeonpox viruses at the surface of infected cells. | chick embryo fibroblasts and chorioallantoic membranes infected with fowlpox virus (fwpv) or pigeonpox virus (ppv) were examined by transmission and scanning electron microscopy. immature virus particles were observed in finely granular areas, i.e. virus factories, of the cytoplasm. these particles had various forms depending on their stages of development. many tubular structures were also seen in these regions. mature virus particles with ellipsoidal or brick-shaped forms enclosing electron-de ... | 2001 | 11347712 |
| response of antioxidative enzymes to plum pox virus in two apricot cultivars. | recent evidence has indicated that activated oxygen species (aos) may function as molecular signals in the induction of defence genes. in the present work, the response of antioxidative enzymes to the plum pox virus (ppv) was examined in two apricot (prunus armeniaca l.) cultivars, which behaved differently against ppv infection. in the inoculated resistant cultivar (goldrich), a decrease in catalase (cat) as well as an increase in total superoxide dismutase (sod) and dehydroascorbate reductase ... | 2001 | 11240915 |
| pathogenicity determinants in the complex virus population of a plum pox virus isolate. | several subisolates were separated from a single plum pox virus (ppv) isolate, ppv-ps. in spite of an extremely high sequence conservation (more than 99.9% similarity), different subisolates differed largely in pathogenicity in herbaceous hosts and infectivity in woody plants. the severity of symptomatology did not seem to correlate with virus accumulation. sequence analysis and site-directed mutagenesis demonstrated that single amino acid changes in the helper component (hc) protein caused a dr ... | 2001 | 11277425 |
| pilot study of expedited hiv-1 testing of women in labor at an inner-city hospital in new york city. | to evaluate the feasibility of offering voluntary counseling and expedited human immunodeficiency virus (hiv) testing to women in labor, and to assess the characteristics of two rapid hiv assays compared with results from an expedited standard enzyme immunoassay (eia), with western blot confirmation, as indicated, we undertook a pilot study immediately prior to enactment of new york state regulations (august 1999) requiring expedited testing of laboring women (or newborns) with undocumented hiv ... | 2001 | 11321245 |
| replacement of the coat protein gene of plum pox potyvirus with that of zucchini yellow mosaic potyvirus: characterization of the hybrid potyvirus. | infectious hybrid virus was generated by replacing part of the coat protein gene of plum pox potyvirus with that of the zucchini yellow mosaic potyvirus. this viable hybrid contains 84.5% of zucchini yellow mosaic potyvirus coat protein gene while the rest of the sequence was derived from plum pox potyvirus. changing the coat protein gene between these two viruses had no effect on the experimental host range. pathogenicity, stability and replication capacity of the hybrid virus were nearly ident ... | 2001 | 11376842 |
| post-transcriptional gene silencing in plum pox virus resistant transgenic european plum containing the plum pox potyvirus coat protein gene. | transgenic plums containing the plum pox potyvirus coat protein (ppv-cp) gene were inoculated with ppv. infection was monitored by evaluating symptoms, elisa, and ic-rt-pcr. transgenic clone c5 was highly resistant to ppv during four years of testing and displayed characteristics typical of post-transcriptional gene silencing (ptgs), including a high level of transgene transcription in the nucleus, low levels of transgene mrna in the cytoplasm, a complex multicopy transgene insertion with aberra ... | 2001 | 11437277 |
| clinical diagnosis of influenza virus infection: evaluation of diagnostic tools in general practice. | with the development of new antiviral agents for influenza, the urge for rapid and reliable diagnosis of influenza becomes increasingly important. respiratory virus infections are difficult to distinguish on clinical grounds. general practitioners (gps) however, still depend on their clinical judgement. | 2001 | 11510391 |
| protection of rabbits against rabbit hemorrhagic disease virus by immunization with the vp60 protein expressed in plants with a potyvirus-based vector. | a new plum pox potyvirus (ppv)-based vector has been constructed for the expression of full-length individual foreign proteins. the foreign sequences are cloned between the nib replicase and capsid protein (cp) cistrons. the heterologous protein is split from the rest of the potyviral polyprotein by cleavage at the site that originally separated the nib and cp proteins and at an additional nia protease recognition site engineered at its amino-terminal end. this vector (ppv-nk) has been used to c ... | 2001 | 11162842 |
| integrated rt-pcr/nested pcr diagnosis for differentiating between subgroups of plum pox virus. | an rt-pcr/nested pcr technique was developed for the simultaneous detection and typing of plum pox virus (ppv) and its major types--dideron (d), marcus (m), el-amar (ea) and cherry (c). degenerated oligonucleotides were synthesized for the general detection of ppv, flanking the coding sequence for the n-terminal portion of the coat protein (cp), within which strain-specific differences were identified. on the basis of these characteristic differences, degenerated primer pairs were designed to di ... | 2001 | 11226563 |
| identification of an n-terminal domain of the plum pox potyvirus ci rna helicase involved in self-interaction in a yeast two-hybrid system. | potyvirus ci rna helicase is a protein involved in rna genome replication and virus movement. the protein aggregates in the cytoplasm of infected cells to form typical cylindrical inclusions. a yeast two-hybrid system was used to analyse interactions of the ci rna helicase from plum pox potyvirus (ppv) with itself and with other viral proteins. no interactions could be detected of full-length ci protein with itself or with ppv p3/6k1, nia, nib or cp proteins. however, positive self-interactions ... | 2001 | 11172111 |
| clinical and pathological observations on pigs with postweaning multisystemic wasting syndrome. | the aim of this work was to characterise the lesions and agents present in clinically normal and clinically affected pigs on a farm during an outbreak of postweaning multisystemic wasting syndrome (pmws), and to evaluate the diagnostic techniques for detecting porcine circovirus type 2 (pcv-2) and other microorganisms. four pigs in the early stage and 11 pigs in the late stage of the disease, and eight clinically normal pigs were necropsied. samples of lymphoid tissue and serum were also obtaine ... | 2001 | 11594382 |
| prevalence of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2 and porcine parvovirus from aborted fetuses and pigs with respiratory problems in korea. | porcine reproductive and respiratory syndrome virus(prrsv)0, porcine circovirus type 2(pcv-2) and porcine parvovirus (ppv)0 infections were investigated as possible causes of the postweaning multisystemic wasting syndrome(pmws). specific primers for rt-pcr and pcr were designed for the differential detection of prrsv, pcv-2 and ppv. using pcr, these viruses were detected in homogenized tissue samples from pigs that had respiratory of reproductive problems in the time period between 1998 and 2000 ... | 2001 | 12441689 |
| incidence of pulmonary tuberculosis and hiv coinfection in the province of seville, spain, 1998. | using the national notifiable disease surveillance system (nndss) and minimum basic data set (mbds) for hospital discharges we evaluated pulmonary tuberculosis (ptb) surveillance and estimated real ptb incidence and human immunodeficiency virus (hiv) coinfection in seville in 1998. in addition, we assessed the sensitivity and the positive predictive value (ppv) of the surveillance system. real incidence of pulmonary tuberculosis was estimated by the capture-recapture method. in 1998, the provinc ... | 2001 | 12086091 |
| comparison of the efficacy of virus inactivation methods in allogeneic avital bone tissue transplants. | several procedures for inactivating viruses are used presently in the context of bone tissue transplants. common methods used are gamma irradiation (25 kgy), treatment with moist heat (82.5 degrees c/15 min., lobator-sd2-system) as well as chemical sterilisation using peracetic acid-ethanol treatment (pes, 2% peracetic acid, 96% ethanol, aqua [2:1:1], 200 mbar, agitation, 4 hours). based on national and international guidelines, we tested the antivirucidal effectiveness of these methods in human ... | 2001 | 15256903 |
| identification of plum pox virus determinants implicated in specific interactions with different prunus spp. | abstract the characterization of pathogenic properties of two infectious clones of plum pox virus (ppv) isolates, pgppv (d group) and pgppvps (m group), was investigated in their woody hosts (seedlings of prunus spp.). the two clones differed in their ability to infect plum and peach cultivars, from no infection to local and systemic infection. the phenotype determinants were located with a set of chimeric viruses from the two clones. in plum, determinants of systemic infection were located in a ... | 2001 | 18944389 |
| zantedeschia mosaic virus causing leaf mosaic symptom in calla lily is a new potyvirus. | anovel virus, zantedeschia mosaic virus (zamv-kr), causing mosaic and malformation symptoms was isolated from calla lily ( zantedeschia spp.) in korea and its biological and molecular properties were characterized. the virus was distinct from dasheen mosaic virus, an araceae-infecting potyvirus, by serological and sequence analyses. multiple alignments of the cp amino acid sequence between the virus and other potyviruses showed 51.8 to 62.1% identity. phylogenetic analyses of the cp revealed tha ... | 2002 | 12491097 |
| immunologic features of porcine circovirus type 2 infection. | clinical expression of porcine circovirus 2 (pcv-2) infection in swine may result in two distinct high mortality disease syndromes. in north america, postweaning multisystemic wasting syndrome (pmws), while still sporadic in incidence, predominates. in europe and elsewhere, both pmws and a second syndrome, porcine dermatitis and nephropathy syndrome (pdns), occur in endemic and epidemic forms. pmws but not pdns has been reproduced in piglets by inoculations with pcv-2 alone or in pcv-2-infected ... | 2002 | 12513928 |
| genetic linkage maps of two apricot cultivars ( prunus armeniaca l.), and mapping of ppv (sharka) resistance. | genetic linkage maps for two apricot cultivars have been constructed using aflp, rapd, rflp and ssr markers in 81 f1 individuals from the cross 'goldrich' x 'valenciano'. this family segregated for resistance to 'plum pox virus' (ppv), the most-important virus affecting prunus species. of the 160 rapd arbitrary primers screened a total of 44 were selected. sixty one polymorphic rapd markers were scored on the mapping population: 30 heterozygous in 'goldrich', 19 heterozygous in 'valenciano', seg ... | 2002 | 12582518 |
| persistent parapoxvirus infection in cattle. | the possibility of persistent parapoxvirus (ppv) infection was investigated by serologically and genetically using cattle infected with the virus experimentally and naturally. three cattle were inoculated with the virus subcutaneously at several spots in the lips and abdominal regions. small papules developed in the inoculated regions, and antibodies to the virus developed and continued persistently. one animal, from which one ppv had been previously isolated, was also subjected to serological a ... | 2002 | 12061631 |
| large scale production and downstream processing of a recombinant porcine parvovirus vaccine. | porcine parvovirus (ppv) virus-like particles (vlps) constitute a potential vaccine for prevention of parvovirus-induced reproductive failure in gilts. here we report the development of a large scale (25 l) production process for ppv-vlps with baculovirus-infected insect cells. a low multiplicity of infection (moi) strategy was efficiently applied avoiding the use of an extra baculovirus expansion step. the optimal harvest time was defined at 120 h post-infection at the moi used, with the cell c ... | 2002 | 12073130 |
| virus safety of a porcine-derived medical device: evaluation of a viral inactivation method. | the goal of this study was to evaluate the efficacy of a virus-inactivating process for use during the preparation of porcine-derived extracellular matrix biomaterials for human clinical implantation. porcine small intestine, the source material for the tissue-engineered, small intestinal submucosa (sis) biomaterial, was evaluated. relevant enveloped, non-enveloped, and model viruses representative of different virus families were included in the investigation: porcine parvovirus (ppv), porcine ... | 2002 | 12115437 |
| endemic hepatitis c virus infection in a sicilian town: further evidence for iatrogenic transmission. | the prevalence of and risk factors for hcv and hbv infections in the general population and the predictive value of alt screening in identifying anti-hcv positive subjects have been evaluated in a small sicilian town. a random 1:4 sampling from the census of the general population was performed. anti-hcv, hcv-rna, hcv genotype, hbsag, and anti-hbc were tested. the linkage between hcv infection and potential risk factors was evaluated by multiple logistic regression analysis. among 721 subjects s ... | 2002 | 12116024 |
| interaction between potyvirus helper component-proteinase and capsid protein in infected plants. | monoclonal antibodies were raised against helper component-proteinase (hcpro) purified from plants infected with the potyvirus lettuce mosaic virus (lmv). these antibodies were used in a two-site triple antibody sandwich elisa assay together with polyclonal antibodies directed against purified virions. an interaction between hcpro and the viral coat protein (cp) was demonstrated in extracts of lmv-infected leaves, as well as for two other potyviruses, plum pox virus and potato virus y. the cp-hc ... | 2002 | 12075097 |
| a longitudinal study of cell-mediated immunity in pigs infected with porcine parvovirus. | porcine parvovirus (ppv) is an ubiquitous pathogen causing reproductive failure in swine. protection against reproductive failure caused by acute ppv infection has commonly been related to the presence of specific antibodies in the dam. however, the role of cell-mediated immunity during chronic ppv infection remains to be elucidated, and may be relevant to the pathogenesis of novel diseases such as postweaning multisystemic wasting syndrome (pmws), which may be triggered by coinfection with ppv ... | 2002 | 12081019 |
| molecular studies of the synergistic interactions between plum pox virus hc-pro protein and potato virus x. | helper component proteinase (hc-pro) is a multifunctional viral protein involved in vection and movement of potyvirus; suppression of host post-transcriptional gene silencing reaction; and synergism of potyvirus with other viruses, notably potexvirus. when the hc-pro of plum pox potyvirus (ppv) was transiently expressed in n. benthamiana and n. clevelandii plants via potato virus x (pvx) vector, a highly synergistic response of leaf necrosis or plant death, was observed in pvxhc-infected plants. ... | 2002 | 12491099 |
| insect cell culture medium supplementation with fetal bovine serum and bovine serum albumin: effects on baculovirus adsorption and infection kinetics. | the effects of insect cell culture medium supplementation with fbs were investigated. bsa was found to be the factor responsible for the increased baculovirus infection rate of fbs-supplemented cultures in a concentration-dependent form up to 25 g l(-)(1). lower rates of baculovirus binding to cells were observed with fbs- and bsa-supplemented cultures compared with infections carried out in serum-free media. virus attachment constants were found to depend on medium matrix composition. an effici ... | 2002 | 12153321 |
| antibodies to selected viral and bacterial pathogens in european wild boars from southcentral spain. | serum samples from 78 european wild boars (sus scrofa) harvested during the 1999-2000 hunting season were tested for antibodies to brucella spp., classical swine fever virus, erysipelothrix rhusiopathiae, haemophilus parasuis, leptospira interrogans serovar pomona, mycoplasma hyopneumoniae, pseudorabies virus (prv), porcine parvovirus (ppv), porcine reproductive and respiratory syndrome virus, salmonella serogroups b, c, and d, streptococcus suis, and swine influenza virus (siv) serotypes h1n1 a ... | 2002 | 12238391 |
| predictive value of alt levels for histologic findings in chronic hepatitis c: a european collaborative study. | the aim of this retrospective study was to determine the predictive value of alanine aminotransferase (alt) levels for histologic findings in patients with chronic hepatitis c virus (hcv) infection. data on 864 hcv rna-positive patients were collected. alt values were obtained at the time of biopsy (before treatment), and normal alt values were defined as normal values obtained at serial evaluations during a 6-month period. histologic results were scored using the metavir system. among all patie ... | 2002 | 12297846 |
| simultaneous and co-operational amplification (co-pcr): a new concept for detection of plant viruses. | a new and highly sensitive method for the amplification of viral rna targets from plant material has been developed and patented. this technique called co-operational amplification (co-pcr) can be carried out easily in a simple tetraprimer reaction based on the simultaneous action of four primers. the reaction process consists of the simultaneous reverse transcription of two different fragments from the same target, one containing the other; the production of four amplicons by the combination of ... | 2002 | 12367729 |
| cd8alpha- cd11b+ dendritic cells present exogenous virus-like particles to cd8+ t cells and subsequently express cd8alpha and cd205 molecules. | recombinant porcine parvovirus virus-like particles (ppv-vlps) are particulate exogenous antigens that induce a strong, specific cytotoxic t lymphocyte (ctl) response in the absence of adjuvant. in the present report, we demonstrate in vivo that dendritic cells (dcs) present ppv-vlps to cd8+ t cells after intracellular processing. ppv-vlps are captured by dcs with a high efficacy, which results in the delivery of these exogenous antigens to 50% of the whole spleen dc population. in vivo, a few h ... | 2002 | 12021304 |
| the etiology and management of genital ulcers in the dominican republic and peru. | clinical diagnosis of genital ulcers is difficult, and diagnostic tests are least available in settings where rates of disease are highest. the world health organization (who) has developed protocols for the syndromic management of genital ulcers in resource-poor settings. however, because risk factors, patterns and causes of disease, and antimicrobial susceptibilities differ from region to region and over time, they must be adapted to local situations. | 2002 | 12370522 |
| integrated process optimization: lessons from retrovirus and virus-like particle production. | the optimization of production and purification processes is usually approached by engineers from a strictly biotechnological point of view. the present paper envisages the definition and application of an optimization model that takes into account the impact of both biological and technological issues upon the optimization protocols and strategies. for this purpose, the optimization of three analogous but different systems comprising animal cell growth and bioparticle production is presented. t ... | 2002 | 12385709 |
| the production of a genus-specific recombinant antibody (scfv) using a recombinant potyvirus protease. | a single chain variable fragment antibody (scfv; anti-nia scfv102) was selected from a synthetic human antibody library by using a nia protease of plum pox virus (ppv) as an antigen, which was expressed in bacteria. the nia protease forms the nuclear inclusion body a and acts as the major protease in the cleavage of the viral polyprotein into functional proteins. the nia protein was detected with anti-nia scfv102 after expression in escherichia coli cells as well as from ppv-infected nicotiana b ... | 2002 | 12393153 |
| viral pharmacovigilance study of haemophiliacs receiving porcine factor viii. | porcine factor viii (fviii; hyate:c; speywood biopharm ltd, uk) has been used since 1980 for the treatment both of patients with acquired haemophilia and those with congenital haemophilia and inhibitory antibodies. each batch is extensively screened using cell-culture techniques to confirm the absence of viruses. the production process does not incorporate specific virucidal treatment steps, such as heat treatment or the addition of a solvent/detergent mixture. low levels of porcine parvovirus w ... | 2002 | 12410650 |
| identification of immunogenic hot spots within plum pox potyvirus capsid protein for efficient antigen presentation. | pepscan analysis has been used to characterize the immunogenic regions of the capsid protein (cp) in virions of plum pox potyvirus (ppv). in addition to the well-known highly immunogenic n- and c-terminal domains of cp, regions within the core domain of the protein have also shown high immunogenicity. moreover, the n terminus of cp is not homogeneously immunogenic, alternatively showing regions frequently recognized by antibodies and others that are not recognized at all. these results have help ... | 2002 | 12438590 |
| infectious agents identified in pigs with multifocal interstitial nephritis at slaughter. | one kidney was taken from each of 100 pigs at slaughter; 50 had gross lesions of multifocal interstitial nephritis and 50 had no gross lesions. forty-nine of the affected kidneys had lesions that were characterised by the presence of either a few randomly distributed or numerous widely disseminated pale foci, 1 to 3 mm in diameter, on the cortical surface (white-dotted kidneys). microscopically, these focal inflammatory lesions often had a distinct lymphofollicular pattern (follicular nephritis) ... | 2002 | 11871667 |
| the capsid protein of a plant single-stranded rna virus is modified by o-linked n-acetylglucosamine. | plum pox virus (ppv) is a member of the potyvirus genus of plant viruses. labeling with udp-[3h]galactose and galactosyltransferase indicated that the capsid protein (cp) of ppv is a glycoprotein with n-acetylglucosamine terminal residues. mass spectrometry analysis of different ppv isolates and mutants revealed o-linked n-acetylglucosamination, a modification barely studied in plant proteins, of serine and/or threonine residues near the amino end of ppv cp. cp of ppv virions is also modified by ... | 2002 | 11606576 |
| parapoxviruses: from the lesion to the viral genome. | viruses of the genus parapoxvirus from the family poxviridae cause widespread but localized diseases of small and large ruminants. the economically most important disease is contagious pustular dermatitis or contagious ecthyma among sheep and goats, often simply called orf. the parapoxviruses (ppv) can be transmitted to man leading to localized lesions that are named pseudocowpox or milkers' node as being mostly restricted to the hands and fingers. in cattle two forms of ppv manifestation are co ... | 2002 | 11911596 |
| molecular variability of the p3-6k1 genomic region among geographically and biologically distinct isolates of plum pox virus. | to evaluate molecular variability in the 5' region of the plum pox virus (ppv) genome, a fragment spanning the c-terminal part of p3, the complete 6k1 and start of the ci gene in 12 slovak and french ppv isolates was amplified in ic-rt-pcr and sequenced. computer analysis of obtained and previously published sequences showed rather substantial differences among isolates of ppv-m and d subgroups (percentage of nucleotide identity ranged from 85.65 to 87.68%). there was a relatively low percentage ... | 2002 | 11958455 |
| the structure of porcine parvovirus: comparison with related viruses. | the structure of baculovirus-expressed porcine parvovirus (ppv) capsids was solved using x-ray crystallography and was found to be similar to the related canine parvovirus (cpv) and minute virus of mice (mvm). the ppv capsid protein has 57 % and 49 % amino acid sequence identity with cpv and mvm, respectively, but the degree of conservation of surface-exposed residues is lower than average. consequently, most of the structural differences are on the surface and are the probable cause of the know ... | 2002 | 11827486 |
| host-specific involvement of the hc protein in the long-distance movement of potyviruses. | plum pox virus (ppv) is a member of the potyvirus genus that, in nature, infects trees of the prunus genus. although ppv infects systemically several species of the nicotiana genus, such as n. clevelandii and n. benthamiana, and replicates in the inoculated leaves of n. tabacum, it is unable to infect systemically the last host. the long-distance movement defect of ppv was corrected in transgenic tobacco plants expressing the 5"-terminal region of the genome of tobacco etch virus (tev), a potyvi ... | 2002 | 11799187 |
| how successfully do general practitioners diagnose herpetic gingivo-stomatitis clinically? | herpetic gingivo-stomatitis is a common, often painful affliction of young children. recently, aciclovir treatment has been found to be effective in hospital-referred cases, an approach limited in general practice where laboratory diagnosis is invariably impractical. this study demonstrated that 49 out of 63 clinical diagnoses of herpetic gingivo-stomatitis [ppv = 78%] made by 27 gps were subsequently validated by laboratory virus culture. this suggests that herpetic gingivo-stomatitis might be ... | 2002 | 12051213 |
| comparative serologic and virologic study of commercial swine herds with and without postweaning multisystemic wasting syndrome. | a comparative serologic and virologic study was performed in pigs from 5 herds with postweaning multisystemic wasting syndrome (pmws) and 2 herds without pmws in quebec. in each herd, 60 blood samples were collected at 4-wk intervals from pigs from 3 to 23 wk of age. the serum was evaluated for the presence of antibodies to porcine circovirus type 2 (pcv2) and porcine reproductive and respiratory syndrome virus (prrsv), as well as for the presence of nucleic acid of pcv2, prrsv, an porcine parvo ... | 2003 | 12760476 |
| association of porcine circovirus 2 with porcine respiratory disease complex. | a retrospective study was performed on natural cases of porcine respiratory disease complex (prdc) to determine the association and prevalence of prdc with porcine circovirus 2 (pcv2) and other co-existing pathogens in korea. histologically, alveolar septa were markedly thickened by infiltrates of mononuclear cells. moderate to marked multifocal peribronchial and peribronchiolar fibrosis were present and often extended into the airway lamina propria. among the 105 pigs with prdc, 85 were positiv ... | 2003 | 14550736 |
| risk factors for porcine post-weaning multisystemic wasting syndrome (pmws) in 149 french farrow-to-finish herds. | a cross-sectional study involving 149 farms was carried out in france in 2000 and 2001 to assess the risk factors for post-weaning multisystemic wasting syndrome (pmws). the farms were divided into three groups according to their current or past pmws status: cases (current and typical pmws), controls#1 (pmws-free farms), and controls#2 (farms which have recovered from pmws). two different comparisons were tested: cases versus controls#1 and cases versus controls#2. in the first comparison, the o ... | 2003 | 14554144 |
| genetic variability of porcine parvovirus isolates revealed by analysis of partial sequences of the structural coding gene vp2. | the 3'-terminal 853 nt (and the putative 283 aa) sequence of the vp2-encoding gene from 29 field strains of porcine parvovirus (ppv) were determined and compared both to each other and with other published sequences. sequences were examined using maximum-parsimony and statistical analyses for nucleotide diversity and sequence variability. among the nucleotide sequences of the ppv field strains, 26 polymorphic sites were encountered; 22 polymorphic sites were detected in the putative amino acid s ... | 2003 | 12771420 |
| the major envelope protein, gp5, of a european porcine reproductive and respiratory syndrome virus contains a neutralization epitope in its n-terminal ectodomain. | a set of neutralizing monoclonal antibodies (mabs) directed against the gp(5) protein of european type porcine reproductive and respiratory syndrome virus (prrsv) has been produced previously (weiland et al., 1999). this set reacted with a plaque-purified virus (ppv) subpopulation of dutch isolate intervet-10 (i-10), but not with the european prototype prrsv lv. in order to map the neutralization epitope in the gp(5) protein of the ppv strain, the orf5 nucleotide sequence of ppv was determined. ... | 2003 | 12771423 |
| serum aminotransferase levels and platelet count as predictive factor of fibrosis and cirrhosis in patients with chronic hepatitis c infection. | to assess the ratio of aminotransferases in combination with platelets count as a predictive factor for stages of fibrosis in patients with chronic hepatitis c virus infection. | 2003 | 12779023 |
| expression of self-complementary hairpin rna under the control of the rolc promoter confers systemic disease resistance to plum pox virus without preventing local infection. | homology-dependent selective degradation of rna, or post-transcriptional gene silencing (ptgs), is involved in several biological phenomena, including adaptative defense mechanisms against plant viruses. small interfering rnas mediate the selective degradation of target rna by guiding a multicomponent rnase. expression of self-complementary hairpin rnas within two complementary regions separated by an intron elicits ptgs with high efficiency. plum pox virus (ppv) is the etiological agent of shar ... | 2003 | 12823862 |
| do std clinics correctly diagnose stds? an assessment of std management in hefei, china. | the main purposes of the study were to assess the accuracy of laboratory testing and the diagnosis by physicians in sexually transmitted disease (std) clinics in hefei, china. among 347 men complaining of urethral discharge or dysuria, 240 tested positive at the national centre for either neisseria gonorrhoeae or chlamydia trachomatis, 310 tested positive according to the clinic laboratories, and 347 were diagnosed by the physicians. for chlamydia, the sensitivity and positive predictive value ( ... | 2003 | 14596769 |
| virus concentration using polyethyleneimine-conjugated magnetic beads for improving the sensitivity of nucleic acid amplification tests. | to enhance the sensitivity of virus detection by polymerase chain reaction (pcr) and reverse-transcriptional (rt)-pcr, we developed a novel virus concentration method using polyethyleneimine (pei)-conjugated magnetic beads. pei-magnetic beads adsorbed efficiently the enveloped viruses sindbis virus and herpes simplex 1 virus, and the nonenveloped virus sv-40, but not the nonenveloped viruses porcine parvovirus (ppv) or poliovirus, based on the pcr detection data. furthermore, the infectivity in ... | 2003 | 14599674 |
| the complete nucleotide sequence of plum pox virus isolates from sweet (ppv-swc) and sour (ppv-soc) cherry and their taxonomic relationships within the species. | plum pox virus (ppv) sweet (swc) and sour (soc) cherry isolates were the first ppv isolates to be recovered from natural infection in sweet and sour cherry plants, respectively. their complete nucleotide sequences have been determined finding a deduced genome organisation typical for ppv species. both genomes are 9795 nucleotides long, excluding the 3' terminal poly(a) tail, and contain an open reading frame of 9432 nt, encoding a polyprotein of 3143 amino acids. the nucleotide and predicted ami ... | 2003 | 14579174 |
| generation and characterisation of functional recombinant antibody fragments against rna replicase nib from plum pox virus. | a monoclonal antibody (mab 2a) able to react against the rna replicase nib from plum pox virus (ppv) was obtained and used for generating a specific scfv fragment. the vh and vl coding sequences were cloned and expressed as a fusion scfv protein to alkaline phosphatase. this fusion protein was able to recognise viral nib in both western and tissue-print elisa blots. the affinity and specificity of scfv2a for nib was similar to that of the parental mab and the region yleafy from ppv-nib was ident ... | 2003 | 12535657 |
| an apricot (prunus armeniaca l.) f2 progeny linkage map based on ssr and aflp markers, mapping plum pox virus resistance and self-incompatibility traits. | a genetic linkage map of apricot ( prunus armeniaca l.) was constructed using aflp and ssr markers. the map is based on an f(2) population (76 individuals) derived from self-pollination of an f(1) individual ('lito') originated from a cross between 'stark early orange' and 'tyrinthos'. this family, designated as 'lito' x 'lito', segregated for two important agronomical traits: plum pox virus resistance (ppv) and self-incompatibility. a total of 211 markers (180 aflps, 29 ssrs and two agronomic t ... | 2003 | 12845439 |
| design and function of a conformationally restricted analog of the influenza virus fusion peptide. | a conformationally restricted analog of the n-terminal 12-residue peptide segment of the ha2 subunit of the ppv/34, pr/8/34, and jap/57 strains of influenza virus hemagglutinin was synthesized containing three residues of calpha-methyl-valine. this peptide has a higher content of helical structure in the presence of 50% trifluoroethanol or when interacting with liposomes of egg phosphatidylcholine compared with the conformationally more flexible control peptide with the native sequence. the cont ... | 2003 | 12787447 |
| clinical relevance of total hcv core antigen testing for hepatitis c monitoring and for predicting patients' response to therapy. | to study the correlation between total hepatitis c virus (hcv) core antigen (ag) and hcv-rna, and to assess the proficiency of hcv core ag testing in monitoring and predicting virologic response during and after pegylated interferon (peg-ifn) and ribavirin combination therapy. a total of 307 samples from treated and untreated patients were used to assess the correlation between the total hcv core ag test and quantitative hcv-rna assays (superquant, and quantiplex branched dna 2.0 assay). twenty- ... | 2003 | 12823600 |
| elimination of ppv and pnrsv through thermotherapy and meristem-tip culture in nectarine. | the plum pox virus (ppv) and prunus necrotic ringspot virus (pnrsv) cause serious disease problems in stone-fruit trees. in this work, the possibility of obtaining plant material free from these viruses through thermotherapy and meristem-tip culture from infected nectarine shoots (prunus persica var. nectarina max, cv. 'arm king') was studied. in addition, the detection of these viruses in in vitro cultures and young acclimatized plantlets with double antibody sandwich-enzyme-linked immunosorben ... | 2003 | 12898177 |
| [serum antibodies detection for serological diagnosis of severe acute respiratory syndrome]. | to evaluate the significance of serum specific antibodies against severe acute respiratory syndrome (sars) virus in the diagnosis of sars and the concordance of ifa and elisa in the antibodies measurements. | 2003 | 12899765 |
| is hsv serology useful for the management of first episode genital herpes? | first episode genital herpes simplex virus (hsv) infections can be classified into three groups, primary genital herpes (no previous exposure to hsv), non-primary first episode (igg antibody to hsv of the non-presenting type), and first episode with pre-existing igg hsv antibodies. the use of igm to classify first episode genital herpes has not been evaluated. | 2003 | 12902573 |
| suppressor activity of potyviral and cucumoviral infections in potyvirus-induced transgene silencing. | the process known as 'recovery' by which virus-infected plants become resistant to the infection is an interesting phenomenon where both rna silencing and virus resistance fully converge. in a previous study, we showed that transgenic nicotiana benthamiana nibv3 plants, transformed with a mutated nib coding sequence from plum pox virus (ppv), showed a delayed, very specific, resistance phenotype, which was induced by the initial infection. this recovery was the consequence of the activation of a ... | 2003 | 13679623 |
| detection of porcine teschoviruses and enteroviruses by lightcycler real-time pcr. | porcine picornaviruses comprising at least 23 serotypes grouped into six species were described as causative agents of neurological disorders, reproductive failure, and aphthae-like dermal lesions of swine. other viruses such as classical swine fever virus (csfv), african swine fever virus, pseudorabies virus (prv), vesicular stomatitis virus, vesicular exanthema virus, porcine respiratory and reproductive syndrome virus, and porcine parvovirus (ppv) may cause diseases with similar clinical symp ... | 2003 | 14500127 |
| virus concentration using sulfonated magnetic beads to improve sensitivity in nucleic acid amplification tests. | to enhance the sensitivity of virus detection by polymerase chain reaction (pcr) and reverse-transcriptional (rt)-pcr, we developed a novel virus-concentration method using sulfonated (so-) magnetic beads in the presence of divalent cations. in the presence of either zn(2+) or cu(2+) ions, we showed that so-magnetic beads were able to concentrate non-enveloped model viruses, such as porcine parvovirus (ppv) and poliovirus, which were not concentrated by polyethyleneimine (pei)-magnetic beads.(1) ... | 2003 | 12913251 |
| in vivo, dendritic cells can cross-present virus-like particles using an endosome-to-cytosol pathway. | recombinant parvovirus-like particles (ppv-vlps) are particulate exogenous ags that induce strong ctl response in the absence of adjuvant. in the present report to decipher the mechanisms responsible for ctl activation by such exogenous ag, we analyzed ex vivo and in vitro the mechanisms of capture and processing of ppv-vlps by dendritic cells (dcs). in vivo, ppv-vlps are very efficiently captured by cd8alpha- and cd8alpha+ dcs and then localize in late endosomes of dcs. macropinocytosis and lip ... | 2003 | 12928368 |
| fluorescent labelling reveals spatial separation of potyvirus populations in mixed infected nicotiana benthamiana plants. | the distribution of potyviruses in mixed infected nicotiana benthamiana plants was investigated by using green and red fluorescent proteins (gfp, dsred). full-length cdna clones of plum pox virus (ppv-nat-agfps; ppv-nat-red), tobacco vein mottling virus (tvmv-gfp; tvmv-red) and clover yellow vein virus (clyvv-gfp) expressing fluorescent proteins, referred to here as labelled viruses, were used to characterize the distribution of different potyviral populations (e.g. tvmv-gfp/ppv-nat-red), as wel ... | 2003 | 13679622 |
| whole blood samples as an alternative to serum for detection of immunity to measles virus by elisa. | the aim of this study was to evaluate enzyme linked immunosorbent assay (elisa) as a testing strategy for detection of antibodies against measles virus from microquantities of blood soaked onto filter paper. we studied 165 healthy children in the age group of 1 to 2 years, attending the outpatient department of pediatrics. two sets of samples were collected from each child. one by venipuncture and the other on whatman filter paper-3 discs of 20 mm size by finger or heel prick so that each strip ... | 2003 | 14711476 |