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morphometric analysis of postoperative corneal neovascularization after high-risk keratoplasty: herpetic versus non-herpetic disease.postoperative complications after high-risk corneal grafting are decisively associated with corneal neovascularization (cnv). this study aimed to identify the incidence, extent, speed, localization, and influence of surgery-related factors on cnv after high-risk penetrating keratoplasty (pk) and to evaluate the effect of removing the angiogenic stimulus, i.e., residual components of herpes simplex virus (hsv) on postkeratoplasty cnv in patients with herpetic stromal keratitis (hsk).201222419035
a survey of resistance to tomato bushy stunt virus in the genus nicotiana reveals that the hypersensitive response is triggered by one of three different viral proteins.in this study, we screened 22 nicotiana spp. for resistance to the tombusviruses tomato bushy stunt virus (tbsv), cucumber necrosis virus, and cymbidium ringspot virus. eighteen species were resistant, and resistance was manifested in at least two different categories. in all, 13 species responded with a hypersensitive response (hr)-type resistance, whereas another five were resistant but either had no visible response or responded with chlorotic lesions rather than necrotic lesions. three diffe ...201323075040
the p33 auxiliary replicase protein of cucumber necrosis virus targets peroxisomes and infection induces de novo peroxisome formation from the endoplasmic reticulum.tombusviruses replicate on pre-existing organelles such as peroxisomes or mitochondria, the membranes of which become extensively reorganized into multivesicular bodies (mvbs) during the infection process. cucumber necrosis virus (cnv) has previously been shown to replicate in association with peroxisomes in yeast. we show that cnv induces mvbs from peroxisomes in infected plants and that gfp-tagged p33 auxiliary replicase protein colocalizes with yfp(skl), a peroxisomal marker. most remarkably, ...201424606690
ccl3l gene copy number and survival in an hiv-1 infected zimbabwean population.the c-c motif chemokine ligand 3-like (ccl3l) protein is a potent chemoattractant which by binding to c-c chemokine receptor type 5 (ccr5) inhibits human immunodeficiency virus (hiv) entry. copy number variation (cnv) of the ccl3l has been shown to be associated with hiv susceptibility and progression to aids, but these results have been inconsistent. we examined a zimbabwean study population for an association of ccl3l cnv with hiv status, progression (cd4 t-cells and viral load), and survival. ...201222484760
reduction of choroidal neovascularization in mice by adeno-associated virus-delivered anti-vascular endothelial growth factor short hairpin rna.strategies leading to the long-term suppression of inappropriate ocular angiogenesis are required to avoid the need for repetitive monthly injections for treatment of diseases of the eye, such as age-related macular degeneration (amd). the present study aimed to develop a strategy for the sustained repression of vascular endothelial growth factor (vegf), which is identified as the key player in exudative amd.201223080553
development of gene therapy for treatment of age-related macular degeneration.intraocular neovascular diseases are the leading cause of blindness in the western world in individuals over the age of 50. age-related macular degeneration (amd) is one of these diseases. exudative amd, the late-stage form, is characterized by abnormal neovessel development, sprouting from the choroid into the avascular subretinal space, where it can suddenly cause irreversible damage to the vulnerable photoreceptor (pr) cells essential for our high-resolution, central vision. the molecular bas ...201424953666
biofilter as a functional annotation pipeline for common and rare copy number burden.recent studies on copy number variation (cnv) have suggested that an increasing burden of cnvs is associated with susceptibility or resistance to disease. a large number of genes or genomic loci contribute to complex diseases such as autism. thus, total genomic copy number burden, as an accumulation of copy number change, is a meaningful measure of genomic instability to identify the association between global genetic effects and phenotypes of interest. however, no systematic annotation pipeline ...201626776200
adeno-associated virus type 8 vector-mediated expression of sirna targeting vascular endothelial growth factor efficiently inhibits neovascularization in a murine choroidal neovascularization model.to assess the feasibility of a gene therapeutic approach to treating choroidal neovascularization (cnv), we generated an adeno-associated virus type 8 vector (aav2/8) encoding an sirna targeting vascular endothelial growth factor (vegf), and determined the aav2/8 vector's ability to inhibit angiogenesis.201424744609
messenger rna- versus retrovirus-based induced pluripotent stem cell reprogramming strategies: analysis of genomic integrity.the use of synthetic messenger rnas to generate human induced pluripotent stem cells (ipscs) is particularly appealing for potential regenerative medicine applications, because it overcomes the common drawbacks of dna-based or virus-based reprogramming strategies, including transgene integration in particular. we compared the genomic integrity of mrna-derived ipscs with that of retrovirus-derived ipscs generated in strictly comparable conditions, by single-nucleotide polymorphism (snp) and copy ...201424736403
subretinal aav2.comp-ang1 suppresses choroidal neovascularization and vascular endothelial growth factor in a murine model of age-related macular degeneration.to assess whether tie2-mediated vascular stabilization ameliorates neovascular age-related macular degeneration (amd), we investigated the impact of adeno-associated virus-mediated gene therapy with cartilage oligomeric matrix protein angiopoietin-1 (aav2.comp-ang1) on choroidal neovascularization (cnv), vascular endothelial growth factor (vegf), and hypoxia-inducible factor (hif) in a mouse model of the disease. we treated mice with subretinal injections of aav2.comp-ang1 or control (aav2.acgfp ...201626775053
gene copy number variations in the leukocyte genome of hepatocellular carcinoma patients with integrated hepatitis b virus dna.integration of hepatitis b virus (hbv) dna into the human liver cell genome is believed to promote hbv-related carcinogenesis. this study aimed to quantify the integration of hbv dna into the leukocyte genome in hepatocellular carcinoma (hcc) patients in order to identify potential biomarkers for hbv-related diseases. whole-genome comparative genomic hybridization (cgh) chip array analyses were performed to screen gene copy number variations (cnv) in the leukocyte genome, and the results were co ...201626769853
encapsidation of host rnas by cucumber necrosis virus coat protein during both agroinfiltration and infection.next-generation sequence analysis of virus-like particles (vlps) produced during agroinfiltration of cucumber necrosis virus (cnv) coat protein (cp) and of authentic cnv virions was conducted to assess if host rnas can be encapsidated by cnv cp. vlps containing host rnas were found to be produced during agroinfiltration, accumulating to approximately 1/60 the level that cnv virions accumulated during infection. vlps contained a variety of host rna species, including the major rrnas as well as cy ...201526269190
aav2 delivery of flt23k intraceptors inhibits murine choroidal neovascularization.long-term inhibition of extracellular vascular endothelial growth factor (vegf) in the treatment of age-related macular degeneration (amd) may induce retinal neuronal toxicity and risk other side effects. we developed a novel strategy which inhibits retinal pigment epithelium (rpe)-derived vegf, sparing other highly sensitive retinal tissues. flt23k, an intraceptor inhibitor of vegf, was able to inhibit vegf in vitro. adeno-associated virus type 2 (aav2)-mediated expression of flt23k was maintai ...201525306972
etiological agents of viral meningitis in children from a dengue-endemic area, southeast region of brazil.meningitis is a disease with a global distribution that constitutes a worldwide burden, with viruses as the primary etiologic agents. the range of viral meningitis severity depends mainly on age, immune status and etiological agent. the aim of this work was to investigate the suspected cases of viral meningitis using molecular techniques to confirm the viral infection. the diagnosed virus was correlated with clinical findings and cytochemical parameters in cerebrospinal liquid (csf) of patients. ...201728320174
genogroup iv and vi canine noroviruses interact with histo-blood group antigens.human noroviruses (hunv) are a significant cause of viral gastroenteritis in humans worldwide. hunv attaches to cell surface carbohydrate structures known as histo-blood group antigens (hbgas) prior to internalization, and hbga polymorphism among human populations is closely linked to susceptibility to hunv. noroviruses are divided into 6 genogroups, with human strains grouped into genogroups i (gi), ii, and iv. canine norovirus (cnv) is a recently discovered pathogen in dogs, with strains class ...201425008923
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