| [transmission cycles of arboviruses in madagascar]. | some arboviruses are highly pathogenic for men or animals. arboviruses epidemiological patterns in madagascar were determined by entomological, serological, and virological surveys. we listed potentials arboviruses vectors in madagascar. entomological results generated by us during five years, as well as prior to, are shown. we caught more than 150,000 hematophagous arthropods, belonging to 107 species at least. 3 of these species were new. 4183 inoculation pools were done. we studied serollogic ... | 1989 | 2576622 |
| three-dimensional structure of theiler murine encephalomyelitis virus (bean strain). | depending on the strain, theiler murine encephalomyelitis virus (tmev) may cause acute encephalitis or chronic demyelinating disease, which is associated with viral persistence in mice. persistent central nervous system infection and demyelination by the less-virulent tmev has provided a useful animal model for the human demyelinating disease multiple sclerosis. the less-virulent bean strain of tmev was crystallized and its atomic structure was determined by x-ray crystallography. the alpha-carb ... | 1992 | 1312722 |
| possible mechanisms in the pathogenesis of virus-induced diabetes mellitus. | insulin-dependent diabetes mellitus results from destruction of pancreatic beta cells. viruses and autoimmunity have been implicated as possible causes of beta cell destruction in genetically predisposed individuals. the evidence for viruses comes largely from experiments in animals, but several studies in humans point to viruses as triggers in the pathogenesis of diabetes in some cases. in animal models, at least 4 different possible mechanisms for virus-induced diabetes have been proposed. the ... | 1987 | 2824113 |
| characterization and specificity of humoral immune responses to theiler's murine encephalomyelitis virus capsid proteins. | humoral antibody responses to theiler's murine encephalomyelitis virus (tmev) capsid proteins were examined. rabbit antisera produced against the native bean strain of tmev and against the isolated capsid proteins (vp1, vp2 and vp3) were tested for their ability to bind or neutralize virus and to inhibit the virus-induced haemagglutination of human o+ erythrocytes. western immunoblotting analysis showed that isolated vp1, vp2 and vp3 each primed for a specific antibody response, but that native ... | 1987 | 2826657 |
| selection and characterization of interferon response mutants from mouse l929 fibroblast cells. | interferon (ifn) response mutants were selected from mouse l929 fibroblast cells and their specific resistance to is-1, an ifn-sensitive mutant of mengovirus, was studied. the standard l cell subline used in our laboratory (g3), is resistant to is-1 infection after pretreatment with low levels of ifn. two clonal sublines that support the growth of is-1 in the presence of ifn (as-4 and ta-6) were isolated from it, and two revertant lines (as-4r1 and ta-6r1) were subsequently selected from as-4 an ... | 1988 | 2828512 |
| dimethyl-10,12-benz(a)acridine; evidence for differential effects on the synthesis of rna of mammalian or avian fibroblasts and some rna viruses. | we have studied the differential effect of dimethyl-10,12-benz(a)acridine (dbmacr) on the synthesis of rna of chicken or mouse fibroblasts in culture and that of some rna-containing viruses such as rous sarcoma virus and mengovirus. dmbacr at low concentrations blocks the cell multiplication of both normal and rous sarcoma virus-transformed chicken fibroblasts in culture; it affects transformed cells more than normal ones. the cell growth inhibiting effect of dmbacr is reversible after short per ... | 1979 | 219894 |
| prediction of three-dimensional models for foot-and-mouth disease virus and hepatitis a virus. | atomic models of foot-and-mouth disease virus and hepatitis a virus have been predicted using amino acid sequence alignments with the known structures of mengo virus and human rhinovirus 14. the structural models are consistent with results of biochemical and immunological studies. the two viruses appear to have surface features exceedingly different than those of other picornaviruses. they also have large hydrophobic cavities within vp1 suggesting that it may be possible to inhibit their infect ... | 1988 | 2845659 |
| studies of two temperature-sensitive mutants of mengo virus. | studies of the synthesis of viral ribonucleates and polypeptides in cells infected with two rna- ts mutants of mengo virus (ts 135 and ts 520) have shown that when ts 135 infected cells are shifted from the permissive (33 degrees c) to the nonpermissive (39 degrees c) temperature: (i) the synthesis of all three species of viral rna (single stranded, replicative form, and replicative intermediate) is inhibited to about the same extent, and (ii) the posttranslational cleavage of structural polypep ... | 1979 | 224989 |
| inhibition by norakin (triperiden) of sindbis virus infection in mice. | intranasal (i. n.) infection with 10 ld50 of sindbis virus caused acute encephalomyelitis and death in abd2f1 mice 3-7 days post infection (p.i.). histologic lesions were found in the cns, pancreas. liver, parotid glands, exorbital lacrimal glands, lymphoid organs and kidneys. repeated oral administration of the anticholinergic anti-parkinson drug norakin protected infected animals from death in a dose-dependent manner when treatment was started prior to but not after virus inoculation. the maxi ... | 1985 | 2864821 |
| failure of high doses of potentially antiviral agents to prevent death in virus-infected mice. | the influence of increasing doses of various potentially antiviral agents on mengo virus-and sindbis virus-infected mice was assessed determining death rates and histologic lesions. all drugs given in abundance showed dose-dependent decrease of antiviral activity following the maximum protective effects in either animal model. from the toxicological point of view the antiviral agents in question could be classified into two groups. high doses of tilorone hydrochloride or 10-carboxymethyl-9-acrid ... | 1985 | 2864822 |
| dynamic model of the pathogenesis of mengo virus infection in mice. | a mathematical model of the pathogenesis of experimental mengo virus infection in mice has been developed and fitted using kinetic data of both virus multiplication in different organs and mortality. the behaviour of the model proved to be bistable. in contrast to the widely accepted hypothesis that an acutely virus-infected host dies when virus replication has attained a critical level in the main target organ, the present results showed the following: the maximum virus titre in brain, the main ... | 1987 | 2892380 |
| [arboviroses in the region of nosy-bé, madagascar. serologic and entomologic data]. | since 1977, the pasteur institute of madagascar has been studying, during six surveys, the arboviruses of nosy-be area, in the north-west of madagascar. 47.2% out of 271 human sera and 11.3% out of 151 sera of lemurs, tested for antibodies to 16 arboviruses by the haemagglutination inhibition test, are positive. the results show an important prevalence of flaviviruses. west nile and dengue 1 viruses were probably circulating some years before the surveys. antibodies against sindbis and rift vall ... | 1988 | 2900082 |
| evidence that ifn-alph/beta induces two antiviral states active against different viruses. | ifn-alpha/beta has been suggested to require only one round of mrna and protein synthesis to induce an antiviral state. we have examined the mechanism of induction of the antiviral states shown against three types of viruses: mengovirus (plus strand, sense rna), vesicular stomatitis virus (vsv, minus strand rna), and vaccinia virus (dna). mouse l cells were treated with ifn-alpha/beta and cycloheximide and then with actinomycin d on a schedule which allowed only one round of mrna and protein syn ... | 1985 | 2987400 |
| evidence for the presence of an inhibitor on ribosomes in mouse l cells infected with mengovirus. | after infection of mouse l cells with mengovirus, there is a rapid inhibition of protein synthesis, a concurrent disaggregation of polysomes, and an accumulation of 80s ribosomes. these 80s ribosomes could not be chased back into polysomes under an elongation block. the infected-cell 80s-ribosome fraction contained twice as much initiator methionyl-trna and mrna as the analogous fraction from uninfected cells. since the proportion of 80s ribosomes that were resistant to pronase digestion also in ... | 1985 | 2993647 |
| [in vitro effectiveness of new antiviral substances. 1. results of screening studies against selected picornaviruses]. | a number of 3108 new synthetic compounds were subjected to a screening for antiviral activity. the screening resulted in the selection of numerous drugs active against mengovirus in vitro. some drugs were also active against coxsackie viruses a9 and b1; most of the drugs efficient against coxsackie virus a9 also showed activity against mengovirus. quantitative investigations--such as the determination of a therapeutic index or of the inhibition of infectious virus yields in one-step growth cycle ... | 1985 | 2994288 |
| perspectives on the role of viruses in insulin-dependent diabetes. | insulin-dependent diabetes mellitus (iddm) results from the destruction of pancreatic beta cells. viruses have been suggested as one of the possible causes. the evidence for viruses comes largely from experiments in animals, but several studies in humans also point to viruses as a trigger of this disease in some cases. encephalomyocarditis (emc) virus, mengovirus (2t), and coxsackie b4 virus infect and destroy pancreatic beta cells when inoculated into mice. this results in hypoinsulinemia and h ... | 1985 | 2996865 |
| a comparative study on the translation of cardiovirus rnas in rabbit reticulocyte lysates. | in rabbit reticulocyte lysates the rnas of encephalomyocarditis (emc) virus, mengovirus, and mous-elberfeld (me) virus directed the synthesis of similar sets of products. moreover, the viral protease synthesized from any one of the three viral rnas could cause cleavage of the viral capsid precursor proteins synthesized from any of the three rnas. however, the three rnas differed in their dependence on trna supplementation (to the lysates) for effective translation. in the absence of trna supplem ... | 1985 | 2998057 |
| cytotoxic t lymphocytes and natural killer cell activity in the course of mengo virus infection of mice. | inbred c57bl/6 mice were inoculated intraperitoneally (i.p.) with mengo virus. the activity of cytotoxic t lymphocytes (ctl) and natural killer (nk) cells were measured during the first 22 days following infection. the ctl response began 7 days after virus inoculation, persisted for at least 22 days and was related to the dose of the virus inoculated. nk cell activity was elevated within 24 hr, reached its peak level on the fourth day and declined to normal levels on the eleventh day after expos ... | 1985 | 3000928 |
| toward an in vitro system for picornavirus assembly: purification of mengovirus 14s capsid precursor particles. | mengovirus 14s subviral protein particles generated in infected l cells and in a cell-free translation system primed with mengovirus rna were purified by sucrose gradient centrifugation and immunoaffinity chromatography. the preparations from both sources contained essentially pure proteins epsilon, alpha, and gamma, as was demonstrated in terms of virus-specific proteins (by autoradiography) and total protein content (by silver staining of sodium dodecyl sulfate-polyacrylamide electrophoresis g ... | 1986 | 3001350 |
| antiviral activity towards vsv and mengo virus of a chemically stabilized 2-5a analog upon microinjection into hela cells. | | 1985 | 3008170 |
| the isolation of mengo virus stable non-capsid polypeptides from infected l cells and preliminary characterization of an rna polymerase activity associated with polypeptide e. | isolation of the mengo virus stable non-capsid virus polypeptides e, f, g and i from infected l cells has been achieved. unstable precursors were eliminated by incubation in the presence of pactamycin and capsid polypeptides were removed by ultracentrifugation and affinity chromatography. subsequent sodium dodecyl sulphate (sds)-hydroxylapatite chromatography resolved the non-capsid proteins into two major peaks which comprised f plus g and e plus i, respectively. the individual polypeptide spec ... | 1979 | 230290 |
| independent regulation of the antiviral states induced by muifn-alpha/beta and by muifn-gamma. | the stability profiles of the antiviral states induced in l-929 cells against mengovirus by murine interferons muifn-alpha/beta and by muifn-gamma were shown to be different. treatment of cells with combinations of ifn-alpha/beta and ifn-gamma have previously been shown to result in a potentiated expression of the antiviral state. here we report studies of the stabilities of the antiviral states induced by various combinations of ifn-alpha/beta and ifn-gamma and that the regulation of the antivi ... | 1986 | 3020160 |
| selection and characterization of an interferon-responsive clonal cell line of hela cells. | hela cells generally do not respond well to interferon (ifn). we have used is-1, an ifn-sensitive mutant of mengovirus, to select a clone of ifn-responsive hela cells (f-h12). at moderate levels of human alpha/beta ifn, is-1 yields were fivefold lower in these cells than in similarly protected control cells. in contrast, wild-type mengovirus, vesicular stomatitis virus and a wild-type and thymidine kinase-negative strains of herpes simplex virus type 1 grew equally well in both cell lines. by a ... | 1986 | 3023528 |
| a kinase able to phosphorylate exogenous protein synthesis initiation factor eif-2 alpha is present in lysates of mengovirus-infected l cells. | infection of mouse l929 cells by mengovirus resulted in the expression of a kinase activity that selectively phosphorylated the small, 38,000-molecular-weight subunit of eucaryotic initiation factor 2 and histone h2. this kinase activity was independent of host cell rna synthesis and was located in the postribosomal supernatant (s-100 fraction) early after infection (up to 3 h). at later times after infection (5 h), kinase activity was also associated with the polysome fraction. the kinase prese ... | 1986 | 3023653 |
| the atomic structure of mengo virus at 3.0 a resolution. | the structure of mengo virus, a representative member of the cardio picornaviruses, is substantially different from the structures of rhino- and polioviruses. the structure of mengo virus was solved with the use of human rhinovirus 14 as an 8 a resolution structural approximation. phase information was then extended to 3 a resolution by use of the icosahedral symmetry. this procedure gives promise that many other virus structures also can be determined without the use of the isomorphous replacem ... | 1987 | 3026048 |
| structural and physiological properties of mengovirus: avirulent, hemagglutination-defective mutants express altered alpha (1 d) proteins and are adsorption-defective. | structural and physiological properties of two mutants of mengovirus, 205 and 280, were compared to those of wild-type virus to understand the molecular basis of changes exhibited in their biological function. two dimensional gel electrophoresis of wild-type and mutant structural proteins revealed alterations in the isoelectric character of the alpha (1 d) protein of both mutant 205 and 280. these data suggest that alterations in the alpha (1 d) protein may be responsible for the phenotypic chan ... | 1987 | 3028339 |
| translational control: recognition of the methylated 5' end and an internal sequence in eukaryotic mrna by the initiation factor that binds methionyl-trnafmet. | structural analogs of the methylated 5' end (cap) of eukaryotic mrna, such as 7-methylguanosine 5'-monophosphate, specifically inhibit both gtp-dependent binding of met-trnafmet and binding of globin mrna to eukaryotic initiation factor 2 (eif-2). addition of purified eif-2 effectively relieves the cap analog-induced inhibition of globin mrna translation. the analog competitively inhibits the function of eif-2 and of mrna in protein synthesis. binding to eif-2 of capped mrna as well as noncapped ... | 1978 | 273228 |
| the effect of mengovirus infection on lipid synthesis in cultured ehrlich ascites tumor cells. | the concept of generally increased lipid synthesis during the initial 2/3 of picornaviral infectious cycles, held by several authors, needs differentiation. in mengovirus-infected ehrlich ascites tumor cells, an increase in the rate of synthesis of phosphatidylcholine could be confirmed, but for phosphatidylethanolamine constant to decreasing rates of synthesis were found. moreover, phosphatidylinositol was increasingly synthesized in the midst of the infectious cycle. the changes observed might ... | 1987 | 3031411 |
| neutralizing antibody to mengo virus, induced by synthetic peptides. | a peptide from the carboxyl-terminal region of the mengo virus capsid protein vp1, representing residues 259 to 277, can induce serum neutralizing (sn) antibodies in both the mouse and guinea-pig. this peptide, termed f164, also induces high levels of protective neutralizing antibodies in mice subsequent to immunization; 87 to 100% of mice are refractory to the effects of an intraperitoneal challenge of 100 ld50 of mengo virus. the mouse model discussed herein will prove useful for studying the ... | 1991 | 1709682 |
| analysis of the complete nucleotide sequence of the picornavirus theiler's murine encephalomyelitis virus indicates that it is closely related to cardioviruses. | theiler's murine encephalomyelitis viruses (tmev) are naturally occurring enteric pathogens of mice which constitute a separate serological group within the picornavirus family. persistent tmev infection in mice provides a relevant experimental animal model for the human demyelinating disease multiple sclerosis. to provide information about the tmev classification, genome organization, and protein processing map, we determined the complete nucleotide sequence of the tmev genome and deduced the a ... | 1987 | 3033278 |
| synergistic action of quercetin and murine alpha/beta interferon in the treatment of mengo virus infection in mice. | abd2f1 mice were infected intraperitoneally (i.p.) or intranasally (i.n.) with mengo or sindbis virus and treated with either crude murine alpha/beta interferon (muifn-alpha/beta) or quercetin, or both. muifn-alpha/beta given i.p. or intramuscularly (i.m.) 1-3 h before the infection had a dose-dependent protective effect regardless of the route of administration. when given after the infection, ifn did not show any effect. oral quercetin, capable of protecting cardio, i.e. mengo virus-infected m ... | 1987 | 3038013 |
| catalysis of poliovirus vp0 maturation cleavage is not mediated by serine 10 of vp2. | the maturation of the poliovirus capsid occurs as the result of a single unexplained proteolytic event during which 58 to 59 copies of the 60 vp0 capsid protein precursors are cleaved. an autocatalytic mechanism for cleavage of vp0 to vp4 and vp2 was proposed by arnold et al. (e. arnold, m. luo, g. vriend, m. g. rossman, a. c. palmenberg, g. d. parks, m. j. nicklin, and e. wimmer, proc. natl. acad. sci. usa 84:21-25, 1987) in which serine 10 of vp2 is activated by virion rna to catalyze vp4-vp2 ... | 1991 | 1845893 |
| mutational analysis of regulation of mhc and anti-viral genes. | ctl-mediated selection for loss of expression of mta by h-2-heterozygous sv40-transformed mouse fibroblasts (line 24sv) produced an unusual phenotypic class of maternally transmitted ag negative mutants defective in both mhc expression and in anti-viral activity. severely reduced surface expression of class i mhc ag from multiple loci of both haplotypes correlated with low levels of mhc h chain and beta 2-microglobulin mrna. inasmuch as ifn can up-regulate class i expression and some fibroblasts ... | 1991 | 1848575 |
| [arbovirus infections on the island of nosy-be; serologic and entomologic findings]. | since 1977, the pasteur institute of madagascar has been studying, during six surveys, the arboviruses of nosy-be area, in the north-west of madagascar. 47.2 p. 100 out of 271 human sera and 11.3 p. 100 out of 150 animal sera (mostly from lemurs), tested for antibodies to 16 arboviruses by the haemagglutination inhibition test, are positive. the results show an important prevalence of flaviviruses. west-nile and dengue 1 viruses were probably circulating some years before the surveys. antibodies ... | 1988 | 3242421 |
| effects of environmental factors on the development of insulin-dependent diabetes mellitus. | the development of insulin-dependent diabetes mellitus is thought to be dependent on either the autoimmunity or the interaction of environmental agents with the pancreatic beta cells, or both in a genetically susceptible host. as environmental factors affecting the induction of type i diabetes, diabetogenic chemicals and viruses are likely candidates as primary injurious agents for pancreatic beta cells in man and animal. a number of structurally diverse chemicals including alloxan, streptozotoc ... | 1987 | 3315367 |
| the role of viruses and environmental factors in the induction of diabetes. | the development of iddm results from the destruction of pancreatic beta cells. genetic factors, various immune system alterations, and environmental factors have been studied as the possible causes of iddm. the concordance rate for developing iddm between monozygotic twins approaches 50%, suggesting that genetic factors are necessary, but nongenetic factors such as various immune system alterations and environmental factors also influence the clinical expression of genetic susceptibility. enviro ... | 1990 | 2073786 |
| interferon-gamma is cytotoxic for normal mouse fibroblasts: enhancement by tumor necrosis factor and interleukin 1. | tumor necrosis factor (tnf) and interleukin-1 (il-1) are cytotoxic for certain tumor cells but have a proliferative effect on normal cells. here we show that interferon-gamma (ifn-gamma) can be cytotoxic for normal cells, in particular mouse embryonic fibroblasts. the cytotoxicity effect is observed with immuno-purified recombinant mouse ifn-gamma (muifn-gamma) at concentrations of 1,000 i.u./ml and can be neutralized by anti-muifn-gamma monoclonal antibodies. the effect appears 48 h after initi ... | 1989 | 2497277 |
| cellular changes attending mengovirus-induced cytolysis of mouse l-cells. | | 1971 | 4108194 |
| structure and infectivity of picornaviral rna encapsidated by cowpea chlorotic mottle virus protein. | poliovirus and mengo virus rna were shown to associate efficiently with cowpea chlorotic mottle virus protein to form pseudovirions. the sedimentation coefficient for the pseudovirions was similar to that of poliovirus, and electron microscope observations showed the mengo pseudovirions to be similar in size to mengo virus. such pseudovirions were infectious and were more resistant to ribonuclease than viral rna; however, under our assay conditions, their infectivity was about equal to that of v ... | 1973 | 4128376 |
| the alpha subunit of eucaryotic initiation factor 2 is phosphorylated in mengovirus-infected mouse l cells. | infection of mouse l cells with mengovirus resulted in the activation of a protein kinase (pk) that selectively phosphorylated the small, 38,000-molecular-weight alpha subunit of eucaryotic initiation factor 2 (eif-2) in vitro. the mengovirus-activated kinase was detected in vitro approximately 3 h after virus adsorption. the ratio of phosphorylated to unphosphorylated eif-2 also increased in vivo between 3 and 7 h after adsorption. the virus-activated kinase fractionated with the ribosomal pell ... | 1990 | 2166823 |
| mengo virus maturation is accompanied by c-terminal modification of capsid protein vp1. | we have demonstrated that the c-terminal ends of the vp1 proteins of mengo virus undergo a postassembly trimming of three amino acid residues. a variable proportion of the vp1 molecules isolated from purified virions terminate at glu 277, which corresponds to the initial viral protease 3c cleavage site. the remainder of the vp1 molecules terminates at leu 274. when chymotrypsin treatment is included in the virus purification procedure, leu 274 is the exclusive c-terminal amino acid of the vp1 mo ... | 1989 | 2536988 |
| pathogenicity of mengo virus to mice. i. virological studies. | | 1974 | 4156158 |
| inhibition of rna synthesis in hela and l cells by mengovirus. | | 1967 | 4163399 |
| mengovirus replication in novikoff rat hepatoma and mouse l cells: effects on synthesis of host-cell macromolecules and virus-specific synthesis of ribonucleic acid. | novikoff cells (strain n1s1-67) and l-67 cells, a nutritional mutant of the common strain of mouse l cells which grows in the same medium as n1s1-67 cells, were infected with mengovirus under identical experimental conditions. the synthesis of host-cell ribonucleic acid (rna) by either type of cell was not affected quantitatively or qualitatively until about 2 hr after infection, when viral rna synthesis rapidly displaced the synthesis of cellular rna. the rate of synthesis of protein by both ty ... | 1968 | 4176992 |
| delay of mengovirus-induced cytopathology in mitotic l-cells. | the time course and extent of mengovirus production were the same in metaphase-arrested and interphase l-cells, yet the expression of cytopathology was delayed several hours in metaphase cells. | 1970 | 4194170 |
| mengovirus replication. 3. virus reproduction in chinese hamster ovary cells. | | 1965 | 4283990 |
| mengovirus replication. iv. inhibition of chinese hamster ovary cell division as a result of infection with mengovirus. | | 1965 | 4283992 |
| the effect of mengovirus infection on the activity of the dna-dependent rna polymerase of l-cells. ii. preliminary data on the inhibitory factor. | | 1964 | 4284277 |
| effect of cortisone on activation of lysosomal enzymes resulting from mengovirus infection of l-929 cells. | | 1966 | 4285387 |
| inhibition of mengo virus by interferon. | gauntt, charles j. (the university of texas, austin), and royce z. lockart, jr. inhibition of mengo virus by interferon. j. bacteriol. 91:176-182. 1966.-the inhibition of mengo virus replication in l cells resulting from interferon was studied quantitatively. interferon was titrated on l cells with western equine encephalomyelitis (wee) virus as the challenge virus. one protective unit (pu) of interferon is the least amount of interferon which prevents cytopathic effects when a large multiplicit ... | 1966 | 4285530 |
| the effect of polyanions and polycations on mengo virus--l cell interaction. | | 1965 | 4285585 |
| cloning and synthesis of infectious cardiovirus rnas containing short, discrete poly(c) tracts. | mengovirus rna transcripts with 5' noncoding poly(c) tracts of c8, c12, and c13uc10 have been synthesized in vitro from cdna clones and shown to be infectious to hela cells. a chimeric clone has also been constructed which links the 5' end from one mengovirus clone (299 nucleotides, containing c13uc10) to a 7,424-base fragment derived from the 3' end of encephalomyocarditis (emc) virus. progeny virus isolated after transfection with the clone-derived rnas had the same poly(c) tracts, mengovirus- ... | 1989 | 2538661 |
| [viral growth and interferon production in mice on a starvation diet infected by mengo virus]. | | 1965 | 4286985 |
| symposium on replication of viral nucleic acids. 3. replication of mengovirus ribonucleic acid. | | 1966 | 4287031 |
| structure determination of mengo virus. | the structure of mengo virus was determined to 3.0 a resolution using human rhinovirus 14 as an initial phasing model at 8.0 a resolution. oscillation diffraction photographs were collected at the cornell high energy synchrotron source using orthorhombic mengo virus crystals. the crystal space group was p2(1)2(1)2(1), a = 441.4, b = 427.3 and c = 421.9 a, with one icosahedral particle per asymmetric unit, giving 60-fold noncrystallographic redundancy. the orientations of the four viral particles ... | 1989 | 2559754 |
| replication of mengovirus. ii. general properties of the viral-induced ribonucleic acid polymerase. | plagemann, peter g. w. (western reserve university, cleveland, ohio), and h. earle swim. replication of mengovirus. ii. general properties of the viral-induced ribonucleic acid polymerase. j. bacteriol. 91:2327-2332. 1966.-mengovirus induces the appearance of a ribonucleic acid (rna) polymerase activity in novikoff hepatoma cells which is readily distinguished from the deoxyribonucleic acid (dna)-dependent rna polymerase since it is not inhibited by actinomycin d or deoxyribonuclease, but is inh ... | 1966 | 4287586 |
| respiration of mengovirus-infected l-929 cells. | iglewski, w. j. (the pennsylvania state university, university park), and e. h. ludwig. respiration of mengovirus-infected l-929 cells. j. bacteriol. 92:733-738. 1966.-polarographic techniques were employed to study the oxidative metabolism of l-929 cells during a one-step mengovirus growth cycle. virus maturation began 3.5 hr after infection and was complete with 7 hr. virus maturation was accompanied by a decreased rate of endogenous respiration and an increased rate of oxidation of succinate ... | 1966 | 4288496 |
| cytopathology of mengovirus infection. i. relationship between cellular disintegration and virulence. | | 1967 | 4290639 |
| cytopathology of mengovirus infection. ii. proliferation of membranous cisternae. | | 1967 | 4290640 |
| a sensitive assay for infectivity of mengovirus rna in l cells. | | 1967 | 4291297 |
| passage of mengovirus through the zona pellucida of the mouse morula. | | 1967 | 4291335 |
| statolon-induced resistance of mice to mengovirus. | mice receiving statolon intraperitoneally were 1,000 times more resistant to intraperitoneal challenge with mengovirus than were untreated controls. protection was afforded when statolon was administered 1 day before or 1 day after intraperitoneal inoculation with the virus. no therapeutic effect was observed when treatment with statolon was delayed for 2 days or more after virus infection. exposure of mice to a simulated space cabin environment did not increase their susceptibility to the letha ... | 1967 | 4291957 |
| isolation of a series of mengo virus variants differing in sensitivity to polyanions. | | 1967 | 4291974 |
| physico-chemical studies of three variants of mengo virus and their constituent ribonucleates. | | 1967 | 4292199 |
| sensitivity to, and production of interferon by three variants of mengo virus. | | 1967 | 4292852 |
| influence of heavy water (d2o) on the multiplication of adeno and mengo virus. | | 1967 | 4294141 |
| treatment of mengovirus infection in mice with statolon. | a single intraperitoneal injection of statolon was shown to exert a therapeutic effect on mice previously injected with the large plaque-forming variant of mengovirus. the ld(50) and survival time data demonstrated that such treatment was effective when given 2 to 48 hr after infection. no protective effect was apparent when statolon was administered 3, 4, or 5 days after the viral challenge. it was concluded that statolon, or other similar interferon inducers, may be of therapeutic value in ins ... | 1967 | 4294823 |
| reversible inhibition of induction of mengovirus rna polymerase and of virus maturation in novikoff rat hepatoma cells by phenethyl alcohol. | | 1968 | 4295916 |
| [arbovirus transmission cycles in madagascar]. | some arboviruses are highly pathogenic for men or animals, arboviruses epidemiological patterns in madagascar were determined by entomological, serological, and virological surveys. we listed potentials arboviruses vectors in madagascar. entomological results generated by us during five years, as well as prior to, are shown. we caught more than 150,000 hematophagous arthropods, belonging to 107 species at least. 3 of these species were new. 4183 inoculation pools were done. we studied serologica ... | 1989 | 2751393 |
| effect of statolon-induced interferon on antibody formation. | the effect of statolon-induced interferon on immune response was investigated. young adult swiss albino mice were immunized with chicken red blood cells or infectious mengovirus. the red blood cells were injected three times and the virus twice at 2-week intervals, with or without treatment with statolon 24 hr before the inoculations. the effect of interferon on antibody formation was measured by determinations of hemagglutinating, hemolytic, and virus-neutralizing antibodies in the sera of trea ... | 1968 | 4298812 |
| [characterization of various representatives of the columbia-sk-virus group with fluorescent antibodies. i. the reproduction cycle of mengo virus in l cells]. | | 1967 | 4299732 |
| enhancement of mengovirus nucleic acid infectivity by putrescine. | | 1968 | 4300093 |
| destruction of l cells by mengo virus: use of interferon to study the mechanism. | interferon, when added to l cells, inhibited the synthesis of infectious mengo viral ribonucleic acid, hemagglutinins, and infectious virus by 85 to 95%. serum-blocking antigens were also reduced by the action of interferon, but threefold excess amounts of these antigens accumulated in interferon-treated cultures above the amounts expected for the quantity of infectious virus that was produced in these cultures. radioautographic analysis showed that 28 to 36% of the cells of an interferon-treate ... | 1968 | 4301505 |
| replication of mengovirus in hela cells preinfected with nonreplicating poliovirus. | the replication of mengovirus in hela cells preinfected with poliovirus in the presence of 10(-3) m guanidine was investigated. although host cell protein synthesis is inhibited by the presence of nonreplicating poliovirus, it is found that mengovirus ribonucleic acid (rna) and protein synthesis proceed normally under the same conditions. furthermore, no effects on mengovirus growth by poliovirus can be detected either when mengo protein synthesis is interrupted by acti-dione or when its rna syn ... | 1968 | 4302186 |
| physical and chemical studies of mengo virus variants. ii. chromatographic behavior and chemical composition. | | 1969 | 4304576 |
| the interaction of tritium-labelled mengo virus rna and l cells: the effects of dmso and deae-dextran. | | 1969 | 4305634 |
| utilization of substrate analogs by mengovirus induced rna polymerase. | | 1969 | 4305637 |
| [induction of interferon synthesis by double-stranded rna. i. application to the study of the multiplication cycle of mengo virus]. | | 1969 | 4307590 |
| influence of statolon on resistance of mice to influenza. | various interferon inducers are known to elicit protection against lethal or infecting doses of certain viral agents. because of the relatively high morbidity rate of influenza and its seasonal occurrence, we wished to determine whether statolon-induced interferon might be effective in controlling this disease. mice were treated intraperitoneally with statolon and challenged with influenza a(2) virus by the intranasal route. although interferon was present in the serum at the time of virus admin ... | 1969 | 4308934 |
| mengovirus-induced cytopathic effect in l-cells: protective effect of interferon. | the effect of interferon on mengovirus-induced cytopathic effect (cpe) in l cells, the cut-off of host-cell protein synthesis, and production of mature virus were found to be dependent on the concentration of interferon. cpe and inhibition of host protein synthesis were not affected until the concentration of interferon was increased 100-fold over that required to reduce viral yields by 90%. | 1969 | 4309883 |
| competitive inhibition of uridine incorporation by 6-azauridine in uninfected and mengovirus-infected novikoff hepatoma cells. | | 1969 | 4311690 |
| host-dependent restriction of mengovirus replication. | mengovirus infection of a restrictive cell line, maden's bovine kidney (mdbk), results in a virus yield 1,000-fold less than that obtained from productively infected cell lines such as l cells or ehrlich ascites tumor cells (eat). cells of both types of host systems are infected with comparable efficiencies and are completely killed as a consequence of infection. infective center assays, coupled with the observation of total cell killing, suggest that comparable numbers of cells synthesize viral ... | 1969 | 4311796 |
| insensitivity of mitochondrial rna synthesis to mengovirus infection in cho cells. | | 1969 | 4311805 |
| the step in cellular dna synthesis blocked by newcastle disease or mengovirus infection. | | 1970 | 4312372 |
| [interferon-inducing capacity of double-stranded rna. ii. studies on mengo virus replicatative intermediate]. | | 1970 | 4312964 |
| [fluorescence serology studies on the effects of antiviral agents on virus replication. i. the action of beta-2-dl-thienyl alanine on the replication of mengo virus]. | | 1969 | 4313030 |
| effect of prehumidification on sampling of selected airborne viruses. | studies were undertaken to determine if a prewetting device (humidifier bulb) used in combination with an all glass impinger (agi-30) would increase the recovery of airborne mengovirus-37a, vesicular stomatitis virus (vsv), and the s-13 coliphage. suspensions of t3 coliphage with mengovirus-37a, vsv, or s-13 were aerosolized and collected by using the agi-30-humidifier bulb combination to sample the aerosols before and after shifts in relative humidities (rh). these studies revealed the followin ... | 1969 | 4313171 |
| the structural proteins of mengo virus variants. | | 1970 | 4314506 |
| polyacetal carboxylic acids: a new group of antiviral polyanions. | chlorite-oxidized oxypolysaccharides are polyacetal carboxylic acids. they inhibited the cytopathic effect of vesicular stomatitis virus in mouse embryo cell cultures challenged at low input multiplicity. after intraperitoneal injection of these compounds in mice, interferon appeared in the circulation. the compounds also protected mice against lethal mengovirus infection and against the development of experimental pox lesions on the tail. chlorite-oxidized oxyamylose was antiviral only when at ... | 1970 | 4314553 |
| antiviral activity of chlorite-oxidized oxyamylose, a polyacetal carboxylic acid. | intraperitoneal injection of chlorite-oxidized oxyamylose (coam) protected mice against mengo, vaccinia, semliki forest, and influenza apr8 viruses. topical administration in the eye of rabbits partially inhibited the development of experimental herpetic keratoconjunctivitis. coam resembled polyacrylic acid in many aspects, but it was markedly less toxic. for systemic administration, the therapeutic index was on the order of magnitude of 1:300 to 1:500. although the in vivo antiviral effect of c ... | 1970 | 4314554 |
| physical and chemical studies of mengo virus variants. 3. absorbance--temperature profiles, sedimentation in dextran sulfate gradients, and total-infectious particle ratios. | | 1970 | 4315866 |
| antiviral activity of a chemically stabilized 2-5a analog upon microinjection into hela cells. | 2-5a[ppp(a2'p)n5'a] has been implicated as a mediator in the antiviral action of interferon. its direct evaluation as an indicator of virus replication is hampered by two limitations: its inability to penetrate intact cells, and its rapid intracellular degradation by (2'-5')phosphodiesterase. these problems could be overcome by using a microinjection technique whereby a phosphodiesterase-resistant analog of 2-a, in which the 2'-terminals adenosine residue is replaced by 2-(9-adenyl)-6-hydroxy-me ... | 1986 | 2420642 |
| effect of statolon on aerogenic immunization of mice with mengovirus-37a. | | 1970 | 4317263 |
| circular dichroism studies on mengo virus variants and their constituent ribonucleates. | | 1970 | 4318119 |
| studies of the protein subunit of ph-inactivated mengo virus variants. i. polypeptide composition. | | 1970 | 4318981 |
| mengovirus rna synthesis in productive and restrictive cell lines. | | 1970 | 4318986 |
| influence of blood clearance rates on interferon production and virulence of mengo virus plaque mutants in mice. | | 1970 | 4319089 |
| comparison of agar and agarose preparations for mengovirus plaque formation. | an agarose overlay yielded mengovirus plaques earlier and in greater size and number than overlays of chemically undefined agars with or without enhancers. marked variability in plaque-forming efficacy of commercial agarose preparations was noted. | 1970 | 4320349 |
| growth of mouse l cells in shaken culture and mengovirus plaque formation on l cells suspended in agar. | procedures are described that require a minimum of equipment and maintenance for growing mouse l cells suspended in liquid medium and for plaquing mengovirus on l cells suspended in agar. viability of l cells during storage for 1 to 2 hr at relatively high concentrations was better in media at 30 c than at 0 c, as measured by viable counts after growth for 24 hr at 35 c. the number and size of plaques increased with increasing concentration of nahco(3) in the agar layers, but the relative differ ... | 1970 | 4320864 |
| abnormal dna patterns in animal mitochondria: ethidium bromide-induced breakdown of closed circular dna and conditions leading to oligomer accumulation. | treatment of cultured mouse fibroblasts (l-cells) and hamster kidney cells with ethidium bromide was found to inhibit the incorporation of [(3)h]thymidine into mitochondrial closed-circular dna, but not into nuclear dna. the specific activity of nuclear dna in treated cells was higher than in control cells. treatment also led to breakdown of closed-circular mitochondrial dna, greatly enlarged mitochondrial profiles with few or no cristae, and a higher yield of mitochondrial protein per cell.a si ... | 1970 | 4321352 |